Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 125 |
| Acc16I | TGCGCA | 1 cut(s) | 298 |
| AccB1I | GGYRCC | 1 cut(s) | 484 |
| AccII | CGCG | 1 cut(s) | 246 |
| AciI | CCGC | 5 cut(s) | 51, 109, 130, 244, 353 |
| AclWI | GGATC | 2 cut(s) | 275, 380 |
| AcoI | YGGCCR | 3 cut(s) | 100, 376, 565 |
| AcsI | RAATTY | 1 cut(s) | 490 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 11, 574 |
| AjiI | CACGTC | 2 cut(s) | 85, 172 |
| AjnI | CCWGG | 2 cut(s) | 10, 165 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 144, 176 |
| AluBI | AGCT | 6 cut(s) | 32, 142, 178, 333, 559, 583 |
| AluI | AGCT | 6 cut(s) | 32, 142, 178, 333, 559, 583 |
| AlwI | GGATC | 2 cut(s) | 275, 380 |
| AoxI | GGCC | 3 cut(s) | 100, 376, 565 |
| ApoI | RAATTY | 1 cut(s) | 490 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 391 |
| AspLEI | GCGC | 1 cut(s) | 299 |
| AspS9I | GGNCC | 1 cut(s) | 14 |
| AsuHPI | GGTGA | 1 cut(s) | 59 |
| AvaII | GGWCC | 1 cut(s) | 14 |
| BaeGI | GKGCMC | 1 cut(s) | 401 |
| BanI | GGYRCC | 1 cut(s) | 484 |
| BanII | GRGCYC | 1 cut(s) | 109 |
| BbvCI | CCTCAGC | 1 cut(s) | 546 |
| BccI | CCATC | 2 cut(s) | 352, 512 |
| BceAI | ACGGC | 2 cut(s) | 110, 115 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 490, 524 |
| BciT130I | CCWGG | 2 cut(s) | 12, 167 |
| BciVI | GTATCC | 1 cut(s) | 170 |
| BfaI | CTAG | 1 cut(s) | 584 |
| BfuI | GTATCC | 1 cut(s) | 170 |
| Bme1390I | CCNGG | 2 cut(s) | 12, 167 |
| Bme18I | GGWCC | 1 cut(s) | 14 |
| BmgBI | CACGTC | 2 cut(s) | 85, 172 |
| BmgT120I | GGNCC | 1 cut(s) | 14 |
| BmiI | GGNNCC | 2 cut(s) | 325, 486 |
| BmrFI | CCNGG | 2 cut(s) | 12, 167 |
| Bpu10I | CCTNAGC | 2 cut(s) | 459, 546 |
| BsaJI | CCNNGG | 2 cut(s) | 165, 213 |
| BsaWI | WCCGGW | 1 cut(s) | 320 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 11, 574 |
| BseBI | CCWGG | 2 cut(s) | 12, 167 |
| BseDI | CCNNGG | 2 cut(s) | 165, 213 |
| BseGI | GGATG | 2 cut(s) | 87, 523 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 11, 574 |
| BseMII | CTCAG | 1 cut(s) | 537 |
| BseRI | GAGGAG | 5 cut(s) | 83, 170, 379, 382, 439 |
| BseSI | GKGCMC | 1 cut(s) | 401 |
| BseX3I | CGGCCG | 2 cut(s) | 100, 376 |
| Bsh1236I | CGCG | 1 cut(s) | 246 |
| Bsh1285I | CGRYCG | 2 cut(s) | 103, 379 |
| BshFI | GGCC | 3 cut(s) | 102, 378, 567 |
| BshNI | GGYRCC | 1 cut(s) | 484 |
| BsiEI | CGRYCG | 2 cut(s) | 103, 379 |
| BsiSI | CCGG | 1 cut(s) | 321 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 11, 574 |
| BsnI | GGCC | 3 cut(s) | 102, 378, 567 |
| Bsp1286I | GDGCHC | 2 cut(s) | 109, 401 |
| Bsp143I | GATC | 2 cut(s) | 267, 372 |
| BspACI | CCGC | 5 cut(s) | 51, 109, 130, 244, 353 |
| BspANI | GGCC | 3 cut(s) | 102, 378, 567 |
| BspCNI | CTCAG | 1 cut(s) | 538 |
| BspFNI | CGCG | 1 cut(s) | 246 |
| BspLI | GGNNCC | 2 cut(s) | 325, 486 |
| BspPI | GGATC | 2 cut(s) | 275, 380 |
| BspT107I | GGYRCC | 1 cut(s) | 484 |
| BssECI | CCNNGG | 2 cut(s) | 165, 213 |
| BssMI | GATC | 2 cut(s) | 267, 372 |
| Bst2UI | CCWGG | 2 cut(s) | 12, 167 |
| Bst4CI | ACNGT | 2 cut(s) | 71, 593 |
| BstC8I | GCNNGC | 4 cut(s) | 109, 113, 553, 557 |
| BstDEI | CTNAG | 2 cut(s) | 459, 546 |
| BstF5I | GGATG | 2 cut(s) | 87, 523 |
| BstFNI | CGCG | 1 cut(s) | 246 |
| BstHHI | GCGC | 1 cut(s) | 299 |
| BstKTI | GATC | 2 cut(s) | 270, 375 |
| BstMBI | GATC | 2 cut(s) | 267, 372 |
| BstMCI | CGRYCG | 2 cut(s) | 103, 379 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 108 |
| BstNI | CCWGG | 2 cut(s) | 12, 167 |
| BstNSI | RCATGY | 1 cut(s) | 115 |
| BstSCI | CCNGG | 2 cut(s) | 10, 165 |
| BstSLI | GKGCMC | 1 cut(s) | 401 |
| BstUI | CGCG | 1 cut(s) | 246 |
| BstZI | CGGCCG | 2 cut(s) | 100, 376 |
| BsuI | GTATCC | 1 cut(s) | 170 |
| BsuRI | GGCC | 3 cut(s) | 102, 378, 567 |
| BtrI | CACGTC | 2 cut(s) | 85, 172 |
| BtsCI | GGATG | 2 cut(s) | 87, 523 |
| BtsIMutI | CAGTG | 1 cut(s) | 589 |
| Cac8I | GCNNGC | 4 cut(s) | 109, 113, 553, 557 |
| CfoI | GCGC | 1 cut(s) | 299 |
| Cfr13I | GGNCC | 1 cut(s) | 14 |
| CviAII | CATG | 2 cut(s) | 112, 149 |
| DdeI | CTNAG | 2 cut(s) | 459, 546 |
| DpnI | GATC | 2 cut(s) | 269, 374 |
| DpnII | GATC | 2 cut(s) | 267, 372 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 125 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 125 |
| EaeI | YGGCCR | 3 cut(s) | 100, 376, 565 |
| EagI | CGGCCG | 2 cut(s) | 100, 376 |
| EciI | GGCGGA | 1 cut(s) | 368 |
| EclXI | CGGCCG | 2 cut(s) | 100, 376 |
| Eco24I | GRGCYC | 1 cut(s) | 109 |
| Eco47I | GGWCC | 1 cut(s) | 14 |
| Eco52I | CGGCCG | 2 cut(s) | 100, 376 |
| EcoRII | CCWGG | 2 cut(s) | 10, 165 |
| EcoT38I | GRGCYC | 1 cut(s) | 109 |
| FaeI | CATG | 2 cut(s) | 115, 152 |
| FaiI | YATR | 4 cut(s) | 113, 150, 264, 604 |
| FatI | CATG | 2 cut(s) | 111, 148 |
| FauI | CCCGC | 2 cut(s) | 58, 116 |
| FokI | GGATG | 2 cut(s) | 74, 530 |
| FriOI | GRGCYC | 1 cut(s) | 109 |
| FspBI | CTAG | 1 cut(s) | 584 |
| FspI | TGCGCA | 1 cut(s) | 298 |
| GlaI | GCGC | 1 cut(s) | 298 |
| HaeIII | GGCC | 3 cut(s) | 102, 378, 567 |
| HapII | CCGG | 1 cut(s) | 321 |
| HhaI | GCGC | 1 cut(s) | 299 |
| Hin1II | CATG | 2 cut(s) | 115, 152 |
| Hin6I | GCGC | 1 cut(s) | 297 |
| HinP1I | GCGC | 1 cut(s) | 297 |
| HinfI | GANTC | 3 cut(s) | 19, 317, 596 |
| HpaII | CCGG | 1 cut(s) | 321 |
| HphI | GGTGA | 1 cut(s) | 59 |
| Hpy166II | GTNNAC | 1 cut(s) | 589 |
| Hpy188I | TCNGA | 4 cut(s) | 18, 237, 255, 316 |
| Hpy188III | TCNNGA | 2 cut(s) | 281, 439 |
| Hpy8I | GTNNAC | 1 cut(s) | 589 |
| Hpy99I | CGWCG | 1 cut(s) | 10 |
| HpyAV | CCTTC | 3 cut(s) | 145, 532, 568 |
| HpyCH4III | ACNGT | 2 cut(s) | 71, 593 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 171 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 108 |
| HpyF3I | CTNAG | 2 cut(s) | 459, 546 |
| HpySE526I | ACGT | 2 cut(s) | 84, 171 |
| Hsp92II | CATG | 2 cut(s) | 115, 152 |
| HspAI | GCGC | 1 cut(s) | 297 |
| Kzo9I | GATC | 2 cut(s) | 267, 372 |
| LmnI | GCTCC | 1 cut(s) | 183 |
| LpnPI | CCDG | 9 cut(s) | 24, 152, 179, 203, 266, 334, 343, 569, 581 |
| MaeI | CTAG | 1 cut(s) | 584 |
| MaeII | ACGT | 2 cut(s) | 84, 171 |
| MaeIII | GTNAC | 1 cut(s) | 229 |
| MalI | GATC | 2 cut(s) | 269, 374 |
| MboI | GATC | 2 cut(s) | 267, 372 |
| MboII | GAAGA | 2 cut(s) | 268, 487 |
| MfeI | CAATTG | 1 cut(s) | 292 |
| MhlI | GDGCHC | 2 cut(s) | 109, 401 |
| MluCI | AATT | 3 cut(s) | 292, 478, 490 |
| MroXI | GAANNNNTTC | 1 cut(s) | 391 |
| MspI | CCGG | 1 cut(s) | 321 |
| MspR9I | CCNGG | 2 cut(s) | 12, 167 |
| MunI | CAATTG | 1 cut(s) | 292 |
| MvaI | CCWGG | 2 cut(s) | 12, 167 |
| MvnI | CGCG | 1 cut(s) | 246 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 108 |
| NdeII | GATC | 2 cut(s) | 267, 372 |
| NlaIII | CATG | 2 cut(s) | 115, 152 |
| NlaIV | GGNNCC | 2 cut(s) | 325, 486 |
| NmeAIII | GCCGAG | 2 cut(s) | 128, 238 |
| NmuCI | GTSAC | 1 cut(s) | 229 |
| NsbI | TGCGCA | 1 cut(s) | 298 |
| NspI | RCATGY | 1 cut(s) | 115 |
| PaeI | GCATGC | 1 cut(s) | 115 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 14, 81 |
| PdmI | GAANNNNTTC | 1 cut(s) | 391 |
| PfeI | GAWTC | 3 cut(s) | 19, 317, 596 |
| Psp6I | CCWGG | 2 cut(s) | 10, 165 |
| PspGI | CCWGG | 2 cut(s) | 10, 165 |
| PspN4I | GGNNCC | 2 cut(s) | 325, 486 |
| PspPI | GGNCC | 1 cut(s) | 14 |
| Sau3AI | GATC | 2 cut(s) | 267, 372 |
| Sau96I | GGNCC | 1 cut(s) | 14 |
| ScrFI | CCNGG | 2 cut(s) | 12, 167 |
| SduI | GDGCHC | 2 cut(s) | 109, 401 |
| SinI | GGWCC | 1 cut(s) | 14 |
| SphI | GCATGC | 1 cut(s) | 115 |
| Sse9I | AATT | 3 cut(s) | 292, 478, 490 |
| SsiI | CCGC | 5 cut(s) | 51, 109, 130, 244, 353 |
| SspMI | CTAG | 1 cut(s) | 584 |
| StyD4I | CCNGG | 2 cut(s) | 10, 165 |
| TaaI | ACNGT | 2 cut(s) | 71, 593 |
| TaiI | ACGT | 2 cut(s) | 87, 174 |
| TaqI | TCGA | 3 cut(s) | 138, 270, 438 |
| TasI | AATT | 3 cut(s) | 292, 478, 490 |
| TfiI | GAWTC | 3 cut(s) | 19, 317, 596 |
| TscAI | CASTG | 1 cut(s) | 596 |
| TseFI | GTSAC | 1 cut(s) | 229 |
| Tsp45I | GTSAC | 1 cut(s) | 229 |
| TspDTI | ATGAA | 2 cut(s) | 165, 587 |
| TspGWI | ACGGA | 1 cut(s) | 14 |
| TspRI | CASTG | 1 cut(s) | 596 |
| VpaK11BI | GGWCC | 1 cut(s) | 14 |
| XapI | RAATTY | 1 cut(s) | 490 |
| XceI | RCATGY | 1 cut(s) | 115 |
| XmnI | GAANNNNTTC | 1 cut(s) | 391 |
| XspI | CTAG | 1 cut(s) | 584 |