Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 498 |
| AccB1I | GGYRCC | 1 cut(s) | 498 |
| AccII | CGCG | 1 cut(s) | 474 |
| AclI | AACGTT | 1 cut(s) | 444 |
| AclWI | GGATC | 2 cut(s) | 25, 707 |
| AcoI | YGGCCR | 1 cut(s) | 825 |
| AcsI | RAATTY | 3 cut(s) | 94, 135, 320 |
| AdeI | CACNNNGTG | 1 cut(s) | 270 |
| AfaI | GTAC | 3 cut(s) | 128, 411, 500 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 604 |
| AjnI | CCWGG | 2 cut(s) | 580, 827 |
| AluBI | AGCT | 5 cut(s) | 114, 212, 259, 308, 683 |
| AluI | AGCT | 5 cut(s) | 114, 212, 259, 308, 683 |
| AlwI | GGATC | 2 cut(s) | 25, 707 |
| AoxI | GGCC | 4 cut(s) | 328, 626, 825, 871 |
| ApeKI | GCWGC | 2 cut(s) | 741, 879 |
| ApoI | RAATTY | 3 cut(s) | 94, 135, 320 |
| Asp718I | GGTACC | 1 cut(s) | 498 |
| AspS9I | GGNCC | 2 cut(s) | 328, 778 |
| AsuC2I | CCSGG | 1 cut(s) | 861 |
| AsuHPI | GGTGA | 3 cut(s) | 68, 256, 730 |
| AvaII | GGWCC | 1 cut(s) | 778 |
| BalI | TGGCCA | 1 cut(s) | 827 |
| BanI | GGYRCC | 1 cut(s) | 498 |
| BbvI | GCAGC | 2 cut(s) | 753, 891 |
| BccI | CCATC | 2 cut(s) | 371, 644 |
| BceAI | ACGGC | 1 cut(s) | 886 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 449, 483 |
| BciT130I | CCWGG | 2 cut(s) | 582, 829 |
| BclI | TGATCA | 1 cut(s) | 787 |
| BcnI | CCSGG | 1 cut(s) | 861 |
| BfaI | CTAG | 1 cut(s) | 491 |
| BfmI | CTRYAG | 2 cut(s) | 630, 849 |
| BisI | GCNGC | 2 cut(s) | 742, 880 |
| BlsI | GCNGC | 2 cut(s) | 743, 881 |
| Bme1390I | CCNGG | 3 cut(s) | 582, 829, 861 |
| Bme18I | GGWCC | 1 cut(s) | 778 |
| BmgT120I | GGNCC | 2 cut(s) | 328, 778 |
| BmiI | GGNNCC | 2 cut(s) | 500, 562 |
| BmrFI | CCNGG | 3 cut(s) | 582, 829, 861 |
| BmsI | GCATC | 2 cut(s) | 499, 600 |
| Bpu10I | CCTNAGC | 1 cut(s) | 875 |
| BpuEI | CTTGAG | 1 cut(s) | 745 |
| BpuMI | CCSGG | 1 cut(s) | 861 |
| Bsa29I | ATCGAT | 1 cut(s) | 282 |
| BsaAI | YACGTR | 1 cut(s) | 409 |
| BsaJI | CCNNGG | 1 cut(s) | 580 |
| BsaWI | WCCGGW | 1 cut(s) | 715 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 604 |
| Bse1I | ACTGG | 1 cut(s) | 622 |
| Bse3DI | GCAATG | 1 cut(s) | 357 |
| BseBI | CCWGG | 2 cut(s) | 582, 829 |
| BseCI | ATCGAT | 1 cut(s) | 282 |
| BseDI | CCNNGG | 1 cut(s) | 580 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 604 |
| BseMI | GCAATG | 1 cut(s) | 357 |
| BseNI | ACTGG | 1 cut(s) | 622 |
| BseRI | GAGGAG | 1 cut(s) | 53 |
| BseXI | GCAGC | 2 cut(s) | 753, 891 |
| BseYI | CCCAGC | 1 cut(s) | 437 |
| Bsh1236I | CGCG | 1 cut(s) | 474 |
| BshFI | GGCC | 4 cut(s) | 330, 628, 827, 873 |
| BshNI | GGYRCC | 1 cut(s) | 498 |
| BshVI | ATCGAT | 1 cut(s) | 282 |
| BsiSI | CCGG | 2 cut(s) | 716, 860 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 604 |
| BsnI | GGCC | 4 cut(s) | 330, 628, 827, 873 |
| Bsp1407I | TGTACA | 1 cut(s) | 126 |
| Bsp143I | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| BspANI | GGCC | 4 cut(s) | 330, 628, 827, 873 |
| BspDI | ATCGAT | 1 cut(s) | 282 |
| BspFNI | CGCG | 1 cut(s) | 474 |
| BspLI | GGNNCC | 2 cut(s) | 500, 562 |
| BspMAI | CTGCAG | 1 cut(s) | 853 |
| BspPI | GGATC | 2 cut(s) | 25, 707 |
| BspT107I | GGYRCC | 1 cut(s) | 498 |
| BsrDI | GCAATG | 1 cut(s) | 357 |
| BsrGI | TGTACA | 1 cut(s) | 126 |
| BsrI | ACTGG | 1 cut(s) | 622 |
| BssECI | CCNNGG | 1 cut(s) | 580 |
| BssMI | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| Bst2UI | CCWGG | 2 cut(s) | 582, 829 |
| Bst4CI | ACNGT | 3 cut(s) | 56, 268, 560 |
| BstAUI | TGTACA | 1 cut(s) | 126 |
| BstBAI | YACGTR | 1 cut(s) | 409 |
| BstC8I | GCNNGC | 2 cut(s) | 472, 884 |
| BstDEI | CTNAG | 1 cut(s) | 875 |
| BstFNI | CGCG | 1 cut(s) | 474 |
| BstKTI | GATC | 5 cut(s) | 20, 61, 715, 790, 868 |
| BstMBI | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 476, 848, 879 |
| BstNI | CCWGG | 2 cut(s) | 582, 829 |
| BstNSI | RCATGY | 1 cut(s) | 886 |
| BstSCI | CCNGG | 3 cut(s) | 580, 827, 859 |
| BstSFI | CTRYAG | 2 cut(s) | 630, 849 |
| BstUI | CGCG | 1 cut(s) | 474 |
| BstV1I | GCAGC | 2 cut(s) | 753, 891 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 370 |
| Bsu15I | ATCGAT | 1 cut(s) | 282 |
| BsuRI | GGCC | 4 cut(s) | 330, 628, 827, 873 |
| BsuTUI | ATCGAT | 1 cut(s) | 282 |
| BtsI | GCAGTG | 1 cut(s) | 540 |
| BtsIMutI | CAGTG | 1 cut(s) | 540 |
| Cac8I | GCNNGC | 2 cut(s) | 472, 884 |
| Cfr13I | GGNCC | 2 cut(s) | 328, 778 |
| ClaI | ATCGAT | 1 cut(s) | 282 |
| Csp6I | GTAC | 3 cut(s) | 127, 410, 499 |
| CviAII | CATG | 8 cut(s) | 24, 296, 325, 370, 463, 516, 823, 883 |
| CviQI | GTAC | 3 cut(s) | 127, 410, 499 |
| DdeI | CTNAG | 1 cut(s) | 875 |
| DpnI | GATC | 5 cut(s) | 19, 60, 714, 789, 867 |
| DpnII | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| DraIII | CACNNNGTG | 1 cut(s) | 270 |
| EaeI | YGGCCR | 1 cut(s) | 825 |
| Eco147I | AGGCCT | 1 cut(s) | 628 |
| Eco47I | GGWCC | 1 cut(s) | 778 |
| EcoRI | GAATTC | 2 cut(s) | 135, 320 |
| EcoRII | CCWGG | 2 cut(s) | 580, 827 |
| FaeI | CATG | 8 cut(s) | 27, 299, 328, 373, 466, 519, 826, 886 |
| FatI | CATG | 8 cut(s) | 23, 295, 324, 369, 462, 515, 822, 882 |
| FbaI | TGATCA | 1 cut(s) | 787 |
| Fnu4HI | GCNGC | 2 cut(s) | 742, 880 |
| Fsp4HI | GCNGC | 2 cut(s) | 742, 880 |
| FspBI | CTAG | 1 cut(s) | 491 |
| GluI | GCNGC | 2 cut(s) | 742, 880 |
| GsaI | CCCAGC | 1 cut(s) | 441 |
| HaeIII | GGCC | 4 cut(s) | 330, 628, 827, 873 |
| HapII | CCGG | 2 cut(s) | 716, 860 |
| Hin1II | CATG | 8 cut(s) | 27, 299, 328, 373, 466, 519, 826, 886 |
| HincII | GTYRAC | 1 cut(s) | 9 |
| HindII | GTYRAC | 1 cut(s) | 9 |
| HindIII | AAGCTT | 1 cut(s) | 306 |
| HinfI | GANTC | 4 cut(s) | 247, 594, 721, 772 |
| HpaII | CCGG | 2 cut(s) | 716, 860 |
| HphI | GGTGA | 3 cut(s) | 68, 256, 730 |
| Hpy166II | GTNNAC | 4 cut(s) | 9, 127, 355, 521 |
| Hpy188I | TCNGA | 4 cut(s) | 93, 348, 657, 678 |
| Hpy188III | TCNNGA | 4 cut(s) | 15, 40, 146, 195 |
| Hpy8I | GTNNAC | 4 cut(s) | 9, 127, 355, 521 |
| HpyAV | CCTTC | 1 cut(s) | 449 |
| HpyCH4III | ACNGT | 3 cut(s) | 56, 268, 560 |
| HpyCH4IV | ACGT | 2 cut(s) | 408, 444 |
| HpyCH4V | TGCA | 6 cut(s) | 167, 394, 532, 545, 851, 886 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 476, 848, 879 |
| HpyF3I | CTNAG | 1 cut(s) | 875 |
| HpySE526I | ACGT | 2 cut(s) | 408, 444 |
| Hsp92II | CATG | 8 cut(s) | 27, 299, 328, 373, 466, 519, 826, 886 |
| KpnI | GGTACC | 1 cut(s) | 502 |
| Ksp22I | TGATCA | 1 cut(s) | 787 |
| Kzo9I | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| Lsp1109I | GCAGC | 2 cut(s) | 753, 891 |
| LweI | GCATC | 2 cut(s) | 499, 600 |
| MaeI | CTAG | 1 cut(s) | 491 |
| MaeII | ACGT | 2 cut(s) | 408, 444 |
| MaeIII | GTNAC | 1 cut(s) | 584 |
| MalI | GATC | 5 cut(s) | 19, 60, 714, 789, 867 |
| MboI | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| MboII | GAAGA | 4 cut(s) | 78, 164, 713, 716 |
| MlsI | TGGCCA | 1 cut(s) | 827 |
| MluCI | AATT | 6 cut(s) | 94, 135, 159, 320, 384, 417 |
| MluNI | TGGCCA | 1 cut(s) | 827 |
| MmeI | TCCRAC | 1 cut(s) | 585 |
| MnlI | CCTC | 7 cut(s) | 22, 74, 142, 342, 512, 618, 671 |
| Mox20I | TGGCCA | 1 cut(s) | 827 |
| MscI | TGGCCA | 1 cut(s) | 827 |
| MseI | TTAA | 4 cut(s) | 171, 180, 237, 555 |
| MslI | CAYNNNNRTG | 1 cut(s) | 368 |
| Msp20I | TGGCCA | 1 cut(s) | 827 |
| MspA1I | CMGCKG | 1 cut(s) | 212 |
| MspI | CCGG | 2 cut(s) | 716, 860 |
| MspR9I | CCNGG | 3 cut(s) | 582, 829, 861 |
| MvaI | CCWGG | 2 cut(s) | 582, 829 |
| MvnI | CGCG | 1 cut(s) | 474 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 476, 848, 879 |
| NciI | CCSGG | 1 cut(s) | 861 |
| NdeII | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| NlaIII | CATG | 8 cut(s) | 27, 299, 328, 373, 466, 519, 826, 886 |
| NlaIV | GGNNCC | 2 cut(s) | 500, 562 |
| NspI | RCATGY | 1 cut(s) | 886 |
| PaeI | GCATGC | 1 cut(s) | 886 |
| PceI | AGGCCT | 1 cut(s) | 628 |
| PfeI | GAWTC | 4 cut(s) | 247, 594, 721, 772 |
| PkrI | GCNGC | 2 cut(s) | 743, 881 |
| Ppu21I | YACGTR | 1 cut(s) | 409 |
| Psp1406I | AACGTT | 1 cut(s) | 444 |
| Psp6I | CCWGG | 2 cut(s) | 580, 827 |
| PspFI | CCCAGC | 1 cut(s) | 437 |
| PspGI | CCWGG | 2 cut(s) | 580, 827 |
| PspN4I | GGNNCC | 2 cut(s) | 500, 562 |
| PspPI | GGNCC | 2 cut(s) | 328, 778 |
| PstI | CTGCAG | 1 cut(s) | 853 |
| PvuII | CAGCTG | 1 cut(s) | 212 |
| RsaI | GTAC | 3 cut(s) | 128, 411, 500 |
| RsaNI | GTAC | 3 cut(s) | 127, 410, 499 |
| RseI | CAYNNNNRTG | 1 cut(s) | 368 |
| SaqAI | TTAA | 4 cut(s) | 171, 180, 237, 555 |
| SatI | GCNGC | 2 cut(s) | 742, 880 |
| Sau3AI | GATC | 5 cut(s) | 17, 58, 712, 787, 865 |
| Sau96I | GGNCC | 2 cut(s) | 328, 778 |
| ScrFI | CCNGG | 3 cut(s) | 582, 829, 861 |
| SfaNI | GCATC | 2 cut(s) | 499, 600 |
| SfcI | CTRYAG | 2 cut(s) | 630, 849 |
| SinI | GGWCC | 1 cut(s) | 778 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 368 |
| SmlI | CTYRAG | 1 cut(s) | 760 |
| SmoI | CTYRAG | 1 cut(s) | 760 |
| SphI | GCATGC | 1 cut(s) | 886 |
| Sse9I | AATT | 6 cut(s) | 94, 135, 159, 320, 384, 417 |
| SseBI | AGGCCT | 1 cut(s) | 628 |
| SspMI | CTAG | 1 cut(s) | 491 |
| StuI | AGGCCT | 1 cut(s) | 628 |
| StyD4I | CCNGG | 3 cut(s) | 580, 827, 859 |
| TaaI | ACNGT | 3 cut(s) | 56, 268, 560 |
| TaiI | ACGT | 2 cut(s) | 411, 447 |
| TaqI | TCGA | 2 cut(s) | 282, 366 |
| TasI | AATT | 6 cut(s) | 94, 135, 159, 320, 384, 417 |
| TatI | WGTACW | 1 cut(s) | 126 |
| TfiI | GAWTC | 4 cut(s) | 247, 594, 721, 772 |
| Tru1I | TTAA | 4 cut(s) | 171, 180, 237, 555 |
| Tru9I | TTAA | 4 cut(s) | 171, 180, 237, 555 |
| TscAI | CASTG | 1 cut(s) | 547 |
| TseI | GCWGC | 2 cut(s) | 741, 879 |
| TspDTI | ATGAA | 7 cut(s) | 17, 39, 313, 333, 410, 504, 651 |
| TspRI | CASTG | 1 cut(s) | 547 |
| VpaK11BI | GGWCC | 1 cut(s) | 778 |
| XapI | RAATTY | 3 cut(s) | 94, 135, 320 |
| XceI | RCATGY | 1 cut(s) | 886 |
| XspI | CTAG | 1 cut(s) | 491 |