protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 143, 413 |
| AccBSI | CCGCTC | 1 cut(s) | 314 |
| AccII | CGCG | 1 cut(s) | 404 |
| AciI | CCGC | 4 cut(s) | 45, 312, 402, 612 |
| AclWI | GGATC | 2 cut(s) | 394, 575 |
| AcsI | RAATTY | 1 cut(s) | 77 |
| AfaI | GTAC | 2 cut(s) | 101, 452 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 143, 413 |
| AgsI | TTSAA | 1 cut(s) | 82 |
| AjiI | CACGTC | 1 cut(s) | 164 |
| AluBI | AGCT | 2 cut(s) | 117, 456 |
| AluI | AGCT | 2 cut(s) | 117, 456 |
| Alw21I | GWGCWC | 1 cut(s) | 169 |
| Alw44I | GTGCAC | 1 cut(s) | 165 |
| AlwI | GGATC | 2 cut(s) | 394, 575 |
| ApaLI | GTGCAC | 1 cut(s) | 165 |
| ApeKI | GCWGC | 1 cut(s) | 548 |
| ApoI | RAATTY | 1 cut(s) | 77 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 77 |
| AspA2I | CCTAGG | 1 cut(s) | 170 |
| AspLEI | GCGC | 2 cut(s) | 226, 406 |
| AsuHPI | GGTGA | 2 cut(s) | 437, 455 |
| AvrII | CCTAGG | 1 cut(s) | 170 |
| BaeGI | GKGCMC | 1 cut(s) | 169 |
| Bbv12I | GWGCWC | 1 cut(s) | 169 |
| BbvI | GCAGC | 1 cut(s) | 535 |
| BceAI | ACGGC | 1 cut(s) | 535 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 603 |
| BfaI | CTAG | 5 cut(s) | 171, 227, 296, 335, 473 |
| BisI | GCNGC | 3 cut(s) | 402, 549, 612 |
| BlnI | CCTAGG | 1 cut(s) | 170 |
| BlpI | GCTNAGC | 1 cut(s) | 197 |
| BlsI | GCNGC | 3 cut(s) | 403, 550, 613 |
| BmgBI | CACGTC | 1 cut(s) | 164 |
| BmsI | GCATC | 2 cut(s) | 28, 142 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 197 |
| BpuEI | CTTGAG | 1 cut(s) | 334 |
| Bsa29I | ATCGAT | 1 cut(s) | 60 |
| BsaJI | CCNNGG | 1 cut(s) | 170 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 414, 444 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 143, 413 |
| Bse3DI | GCAATG | 3 cut(s) | 201, 265, 292 |
| BseCI | ATCGAT | 1 cut(s) | 60 |
| BseDI | CCNNGG | 1 cut(s) | 170 |
| BseGI | GGATG | 1 cut(s) | 133 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 143, 413 |
| BseMI | GCAATG | 3 cut(s) | 201, 265, 292 |
| BseRI | GAGGAG | 1 cut(s) | 137 |
| BseSI | GKGCMC | 1 cut(s) | 169 |
| BseXI | GCAGC | 1 cut(s) | 535 |
| Bsh1236I | CGCG | 1 cut(s) | 404 |
| BshVI | ATCGAT | 1 cut(s) | 60 |
| BsiHKAI | GWGCWC | 1 cut(s) | 169 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 143, 413 |
| BsmI | GAATGC | 1 cut(s) | 462 |
| Bsp1286I | GDGCHC | 1 cut(s) | 169 |
| Bsp1407I | TGTACA | 1 cut(s) | 450 |
| Bsp143I | GATC | 2 cut(s) | 386, 580 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 197 |
| BspACI | CCGC | 4 cut(s) | 45, 312, 402, 612 |
| BspDI | ATCGAT | 1 cut(s) | 60 |
| BspFNI | CGCG | 1 cut(s) | 404 |
| BspHI | TCATGA | 1 cut(s) | 33 |
| BspPI | GGATC | 2 cut(s) | 394, 575 |
| BsrBI | CCGCTC | 1 cut(s) | 314 |
| BsrDI | GCAATG | 3 cut(s) | 201, 265, 292 |
| BsrGI | TGTACA | 1 cut(s) | 450 |
| BssECI | CCNNGG | 1 cut(s) | 170 |
| BssMI | GATC | 2 cut(s) | 386, 580 |
| BssT1I | CCWWGG | 1 cut(s) | 170 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 575 |
| BstAUI | TGTACA | 1 cut(s) | 450 |
| BstC8I | GCNNGC | 1 cut(s) | 49 |
| BstDEI | CTNAG | 2 cut(s) | 118, 197 |
| BstF5I | GGATG | 1 cut(s) | 133 |
| BstFNI | CGCG | 1 cut(s) | 404 |
| BstHHI | GCGC | 2 cut(s) | 226, 406 |
| BstKTI | GATC | 2 cut(s) | 389, 583 |
| BstMBI | GATC | 2 cut(s) | 386, 580 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 575 |
| BstNSI | RCATGY | 1 cut(s) | 51 |
| BstSLI | GKGCMC | 1 cut(s) | 169 |
| BstUI | CGCG | 1 cut(s) | 404 |
| BstV1I | GCAGC | 1 cut(s) | 535 |
| BstX2I | RGATCY | 2 cut(s) | 386, 580 |
| BstYI | RGATCY | 2 cut(s) | 386, 580 |
| Bsu15I | ATCGAT | 1 cut(s) | 60 |
| BsuTUI | ATCGAT | 1 cut(s) | 60 |
| BtrI | CACGTC | 1 cut(s) | 164 |
| BtsCI | GGATG | 1 cut(s) | 133 |
| BtsI | GCAGTG | 1 cut(s) | 584 |
| BtsIMutI | CAGTG | 2 cut(s) | 498, 584 |
| Cac8I | GCNNGC | 1 cut(s) | 49 |
| CciI | TCATGA | 1 cut(s) | 33 |
| CfoI | GCGC | 2 cut(s) | 226, 406 |
| ClaI | ATCGAT | 1 cut(s) | 60 |
| Csp6I | GTAC | 2 cut(s) | 100, 451 |
| CviAII | CATG | 3 cut(s) | 34, 48, 103 |
| CviJI | RGCY | 9 cut(s) | 86, 117, 196, 280, 456, 496, 522, 548, 611 |
| CviKI_1 | RGCY | 9 cut(s) | 86, 117, 196, 280, 456, 496, 522, 548, 611 |
| CviQI | GTAC | 2 cut(s) | 100, 451 |
| DdeI | CTNAG | 2 cut(s) | 118, 197 |
| DpnI | GATC | 2 cut(s) | 388, 582 |
| DpnII | GATC | 2 cut(s) | 386, 580 |
| Eco130I | CCWWGG | 1 cut(s) | 170 |
| Eco32I | GATATC | 1 cut(s) | 274 |
| EcoRI | GAATTC | 1 cut(s) | 77 |
| EcoRV | GATATC | 1 cut(s) | 274 |
| EcoT14I | CCWWGG | 1 cut(s) | 170 |
| ErhI | CCWWGG | 1 cut(s) | 170 |
| FaeI | CATG | 3 cut(s) | 37, 51, 106 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 101, 133, 373, 405 |
| FatI | CATG | 3 cut(s) | 33, 47, 102 |
| FauNDI | CATATG | 1 cut(s) | 439 |
| Fnu4HI | GCNGC | 3 cut(s) | 402, 549, 612 |
| FokI | GGATG | 1 cut(s) | 120 |
| Fsp4HI | GCNGC | 3 cut(s) | 402, 549, 612 |
| FspBI | CTAG | 5 cut(s) | 171, 227, 296, 335, 473 |
| GlaI | GCGC | 2 cut(s) | 225, 405 |
| GluI | GCNGC | 3 cut(s) | 402, 549, 612 |
| HhaI | GCGC | 2 cut(s) | 226, 406 |
| Hin1II | CATG | 3 cut(s) | 37, 51, 106 |
| Hin6I | GCGC | 2 cut(s) | 224, 404 |
| HinP1I | GCGC | 2 cut(s) | 224, 404 |
| HincII | GTYRAC | 1 cut(s) | 435 |
| HindII | GTYRAC | 1 cut(s) | 435 |
| HphI | GGTGA | 2 cut(s) | 437, 455 |
| Hpy166II | GTNNAC | 3 cut(s) | 167, 309, 435 |
| Hpy188I | TCNGA | 3 cut(s) | 8, 192, 325 |
| Hpy188III | TCNNGA | 5 cut(s) | 34, 144, 296, 351, 596 |
| Hpy8I | GTNNAC | 3 cut(s) | 167, 309, 435 |
| HpyAV | CCTTC | 2 cut(s) | 103, 593 |
| HpyCH4IV | ACGT | 2 cut(s) | 27, 163 |
| HpyCH4V | TGCA | 6 cut(s) | 133, 167, 206, 371, 569, 578 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 575 |
| HpyF3I | CTNAG | 2 cut(s) | 118, 197 |
| HpySE526I | ACGT | 2 cut(s) | 27, 163 |
| Hsp92II | CATG | 3 cut(s) | 37, 51, 106 |
| HspAI | GCGC | 2 cut(s) | 224, 404 |
| Kzo9I | GATC | 2 cut(s) | 386, 580 |
| LmnI | GCTCC | 1 cut(s) | 114 |
| LpnPI | CCDG | 5 cut(s) | 74, 129, 140, 420, 471 |
| Lsp1109I | GCAGC | 1 cut(s) | 535 |
| LweI | GCATC | 2 cut(s) | 28, 142 |
| MaeI | CTAG | 5 cut(s) | 171, 227, 296, 335, 473 |
| MaeII | ACGT | 2 cut(s) | 27, 163 |
| MaeIII | GTNAC | 2 cut(s) | 40, 515 |
| MalI | GATC | 2 cut(s) | 388, 582 |
| MbiI | CCGCTC | 1 cut(s) | 314 |
| MboI | GATC | 2 cut(s) | 386, 580 |
| MboII | GAAGA | 3 cut(s) | 284, 502, 584 |
| MfeI | CAATTG | 1 cut(s) | 372 |
| MflI | RGATCY | 2 cut(s) | 386, 580 |
| MhlI | GDGCHC | 1 cut(s) | 169 |
| MluCI | AATT | 5 cut(s) | 77, 176, 241, 372, 573 |
| MmeI | TCCRAC | 1 cut(s) | 161 |
| MnlI | CCTC | 5 cut(s) | 115, 186, 377, 416, 421 |
| MroXI | GAANNNNTTC | 1 cut(s) | 77 |
| MseI | TTAA | 2 cut(s) | 179, 380 |
| MunI | CAATTG | 1 cut(s) | 372 |
| Mva1269I | GAATGC | 1 cut(s) | 462 |
| MvnI | CGCG | 1 cut(s) | 404 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 575 |
| NdeI | CATATG | 1 cut(s) | 439 |
| NdeII | GATC | 2 cut(s) | 386, 580 |
| NlaIII | CATG | 3 cut(s) | 37, 51, 106 |
| NmuCI | GTSAC | 1 cut(s) | 515 |
| NspI | RCATGY | 1 cut(s) | 51 |
| PaeI | GCATGC | 1 cut(s) | 51 |
| PagI | TCATGA | 1 cut(s) | 33 |
| PctI | GAATGC | 1 cut(s) | 462 |
| PdmI | GAANNNNTTC | 1 cut(s) | 77 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 143, 413 |
| PkrI | GCNGC | 3 cut(s) | 403, 550, 613 |
| PsuI | RGATCY | 2 cut(s) | 386, 580 |
| RsaI | GTAC | 2 cut(s) | 101, 452 |
| RsaNI | GTAC | 2 cut(s) | 100, 451 |
| SaqAI | TTAA | 2 cut(s) | 179, 380 |
| SatI | GCNGC | 3 cut(s) | 402, 549, 612 |
| Sau3AI | GATC | 2 cut(s) | 386, 580 |
| SduI | GDGCHC | 1 cut(s) | 169 |
| SetI | ASST | 9 cut(s) | 30, 119, 159, 166, 172, 344, 427, 432, 458 |
| SfaNI | GCATC | 2 cut(s) | 28, 142 |
| SmlI | CTYRAG | 1 cut(s) | 349 |
| SmoI | CTYRAG | 1 cut(s) | 349 |
| SphI | GCATGC | 1 cut(s) | 51 |
| Sse9I | AATT | 5 cut(s) | 77, 176, 241, 372, 573 |
| SsiI | CCGC | 4 cut(s) | 45, 312, 402, 612 |
| SspMI | CTAG | 5 cut(s) | 171, 227, 296, 335, 473 |
| StyI | CCWWGG | 1 cut(s) | 170 |
| TaiI | ACGT | 2 cut(s) | 30, 166 |
| TaqI | TCGA | 3 cut(s) | 22, 60, 595 |
| TasI | AATT | 5 cut(s) | 77, 176, 241, 372, 573 |
| TatI | WGTACW | 2 cut(s) | 99, 450 |
| TauI | GCSGC | 2 cut(s) | 404, 614 |
| Tru1I | TTAA | 2 cut(s) | 179, 380 |
| Tru9I | TTAA | 2 cut(s) | 179, 380 |
| TscAI | CASTG | 2 cut(s) | 505, 591 |
| TseFI | GTSAC | 1 cut(s) | 515 |
| TseI | GCWGC | 1 cut(s) | 548 |
| Tsp45I | GTSAC | 1 cut(s) | 515 |
| TspDTI | ATGAA | 3 cut(s) | 44, 90, 254 |
| TspRI | CASTG | 2 cut(s) | 505, 591 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 143, 413 |
| VneI | GTGCAC | 1 cut(s) | 165 |
| XapI | RAATTY | 1 cut(s) | 77 |
| XbaI | TCTAGA | 1 cut(s) | 295 |
| XceI | RCATGY | 1 cut(s) | 51 |
| XmaJI | CCTAGG | 1 cut(s) | 170 |
| XmnI | GAANNNNTTC | 1 cut(s) | 77 |
| XspI | CTAG | 5 cut(s) | 171, 227, 296, 335, 473 |