Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1523 |
| AccB1I | GGYRCC | 2 cut(s) | 1134, 1523 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 271, 1550 |
| AccII | CGCG | 1 cut(s) | 1082 |
| AciI | CCGC | 2 cut(s) | 219, 520 |
| AclI | AACGTT | 1 cut(s) | 1407 |
| AclWI | GGATC | 4 cut(s) | 143, 735, 846, 1712 |
| AcoI | YGGCCR | 1 cut(s) | 220 |
| AcsI | RAATTY | 4 cut(s) | 495, 586, 690, 982 |
| AcuI | CTGAAG | 1 cut(s) | 885 |
| AfaI | GTAC | 7 cut(s) | 390, 705, 840, 1065, 1525, 1756, 1923 |
| AfeI | AGCGCT | 1 cut(s) | 1342 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 271, 293, 1550, 1833 |
| AflIII | ACRYGT | 5 cut(s) | 480, 1584, 1644, 1699, 1906 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 256 |
| AhlI | ACTAGT | 2 cut(s) | 1153, 1751 |
| AjiI | CACGTC | 1 cut(s) | 1585 |
| AjnI | CCWGG | 2 cut(s) | 170, 1934 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 751, 783 |
| Alw21I | GWGCWC | 2 cut(s) | 1751, 1953 |
| Alw26I | GTCTC | 1 cut(s) | 1575 |
| AlwI | GGATC | 4 cut(s) | 143, 735, 846, 1712 |
| Ama87I | CYCGRG | 1 cut(s) | 398 |
| Aor51HI | AGCGCT | 1 cut(s) | 1342 |
| AoxI | GGCC | 4 cut(s) | 75, 220, 993, 1631 |
| ApeKI | GCWGC | 2 cut(s) | 382, 1246 |
| ApoI | RAATTY | 4 cut(s) | 495, 586, 690, 982 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 407 |
| Asp718I | GGTACC | 1 cut(s) | 1523 |
| AspA2I | CCTAGG | 1 cut(s) | 823 |
| AspLEI | GCGC | 1 cut(s) | 1343 |
| AspS9I | GGNCC | 2 cut(s) | 250, 993 |
| AsuC2I | CCSGG | 1 cut(s) | 379 |
| AsuHPI | GGTGA | 3 cut(s) | 431, 583, 647 |
| AsuII | TTCGAA | 1 cut(s) | 414 |
| AsuNHI | GCTAGC | 1 cut(s) | 1337 |
| AvaI | CYCGRG | 1 cut(s) | 398 |
| AvaII | GGWCC | 1 cut(s) | 250 |
| AvrII | CCTAGG | 1 cut(s) | 823 |
| BanI | GGYRCC | 2 cut(s) | 1134, 1523 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1056, 1088 |
| BbsI | GAAGAC | 1 cut(s) | 232 |
| Bbv12I | GWGCWC | 2 cut(s) | 1751, 1953 |
| BbvI | GCAGC | 2 cut(s) | 369, 1233 |
| BccI | CCATC | 2 cut(s) | 265, 1678 |
| BciT130I | CCWGG | 2 cut(s) | 172, 1936 |
| BclI | TGATCA | 1 cut(s) | 63 |
| BcnI | CCSGG | 1 cut(s) | 379 |
| BcoDI | GTCTC | 1 cut(s) | 1575 |
| BcuI | ACTAGT | 2 cut(s) | 1153, 1751 |
| BfmI | CTRYAG | 1 cut(s) | 847 |
| BfoI | RGCGCY | 1 cut(s) | 1344 |
| BglII | AGATCT | 2 cut(s) | 6, 946 |
| BisI | GCNGC | 4 cut(s) | 220, 383, 521, 1247 |
| BlnI | CCTAGG | 1 cut(s) | 823 |
| BlsI | GCNGC | 4 cut(s) | 221, 384, 522, 1248 |
| BmcAI | AGTACT | 2 cut(s) | 390, 1065 |
| Bme1390I | CCNGG | 3 cut(s) | 172, 379, 1936 |
| Bme18I | GGWCC | 1 cut(s) | 250 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 256 |
| BmeT110I | CYCGRG | 1 cut(s) | 398 |
| BmgBI | CACGTC | 1 cut(s) | 1585 |
| BmgT120I | GGNCC | 2 cut(s) | 250, 993 |
| BmiI | GGNNCC | 4 cut(s) | 994, 1136, 1525, 1933 |
| BmrFI | CCNGG | 3 cut(s) | 172, 379, 1936 |
| BmrI | ACTGGG | 1 cut(s) | 1395 |
| BmsI | GCATC | 5 cut(s) | 168, 434, 1212, 1605, 1929 |
| BmtI | GCTAGC | 1 cut(s) | 1341 |
| BmuI | ACTGGG | 1 cut(s) | 1395 |
| BpiI | GAAGAC | 1 cut(s) | 232 |
| BpmI | CTGGAG | 1 cut(s) | 239 |
| Bpu10I | CCTNAGC | 1 cut(s) | 164 |
| Bpu14I | TTCGAA | 1 cut(s) | 414 |
| BpuEI | CTTGAG | 2 cut(s) | 322, 342 |
| BpuMI | CCSGG | 1 cut(s) | 379 |
| Bsa29I | ATCGAT | 1 cut(s) | 739 |
| BsaAI | YACGTR | 1 cut(s) | 1907 |
| BsaBI | GATNNNNATC | 1 cut(s) | 65 |
| BsaJI | CCNNGG | 2 cut(s) | 397, 823 |
| BsaWI | WCCGGW | 1 cut(s) | 1483 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1738, 1768 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 271, 293, 1550, 1833 |
| Bse118I | RCCGGY | 1 cut(s) | 995 |
| Bse1I | ACTGG | 5 cut(s) | 256, 967, 1401, 1627, 1933 |
| Bse3DI | GCAATG | 1 cut(s) | 1827 |
| Bse8I | GATNNNNATC | 1 cut(s) | 65 |
| BseBI | CCWGG | 2 cut(s) | 172, 1936 |
| BseCI | ATCGAT | 1 cut(s) | 739 |
| BseDI | CCNNGG | 2 cut(s) | 397, 823 |
| BseGI | GGATG | 3 cut(s) | 159, 1924, 1944 |
| BseJI | GATNNNNATC | 1 cut(s) | 65 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 271, 293, 1550, 1833 |
| BseMI | GCAATG | 1 cut(s) | 1827 |
| BseMII | CTCAG | 2 cut(s) | 325, 722 |
| BseNI | ACTGG | 5 cut(s) | 256, 967, 1401, 1627, 1933 |
| BseX3I | CGGCCG | 1 cut(s) | 220 |
| BseXI | GCAGC | 2 cut(s) | 369, 1233 |
| Bsh1236I | CGCG | 1 cut(s) | 1082 |
| Bsh1285I | CGRYCG | 1 cut(s) | 223 |
| BshFI | GGCC | 4 cut(s) | 77, 222, 995, 1633 |
| BshNI | GGYRCC | 2 cut(s) | 1134, 1523 |
| BshVI | ATCGAT | 1 cut(s) | 739 |
| BsiEI | CGRYCG | 1 cut(s) | 223 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1751, 1953 |
| BsiHKCI | CYCGRG | 1 cut(s) | 398 |
| BsiSI | CCGG | 3 cut(s) | 379, 996, 1484 |
| BslFI | GGGAC | 2 cut(s) | 361, 817 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 271, 293, 1550, 1833 |
| BsmAI | GTCTC | 1 cut(s) | 1575 |
| BsmBI | CGTCTC | 1 cut(s) | 1575 |
| BsmFI | GGGAC | 2 cut(s) | 361, 817 |
| BsmI | GAATGC | 1 cut(s) | 276 |
| BsnI | GGCC | 4 cut(s) | 77, 222, 995, 1633 |
| BsoBI | CYCGRG | 1 cut(s) | 398 |
| Bsp119I | TTCGAA | 1 cut(s) | 414 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1751, 1953 |
| Bsp143I | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| BspACI | CCGC | 2 cut(s) | 219, 520 |
| BspANI | GGCC | 4 cut(s) | 77, 222, 995, 1633 |
| BspCNI | CTCAG | 2 cut(s) | 324, 721 |
| BspDI | ATCGAT | 1 cut(s) | 739 |
| BspFNI | CGCG | 1 cut(s) | 1082 |
| BspLI | GGNNCC | 4 cut(s) | 994, 1136, 1525, 1933 |
| BspOI | GCTAGC | 1 cut(s) | 1341 |
| BspPI | GGATC | 4 cut(s) | 143, 735, 846, 1712 |
| BspQI | GCTCTTC | 1 cut(s) | 1510 |
| BspT104I | TTCGAA | 1 cut(s) | 414 |
| BspT107I | GGYRCC | 2 cut(s) | 1134, 1523 |
| BsrDI | GCAATG | 1 cut(s) | 1827 |
| BsrFI | RCCGGY | 1 cut(s) | 995 |
| BsrI | ACTGG | 5 cut(s) | 256, 967, 1401, 1627, 1933 |
| BssAI | RCCGGY | 1 cut(s) | 995 |
| BssECI | CCNNGG | 2 cut(s) | 397, 823 |
| BssMI | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| BssT1I | CCWWGG | 1 cut(s) | 823 |
| Bst2UI | CCWGG | 2 cut(s) | 172, 1936 |
| Bst4CI | ACNGT | 2 cut(s) | 51, 244 |
| Bst6I | CTCTTC | 5 cut(s) | 85, 979, 1510, 1798, 1806 |
| BstBAI | YACGTR | 1 cut(s) | 1907 |
| BstBI | TTCGAA | 1 cut(s) | 414 |
| BstC8I | GCNNGC | 4 cut(s) | 123, 1165, 1339, 1768 |
| BstDEI | CTNAG | 7 cut(s) | 34, 89, 164, 311, 708, 771, 1414 |
| BstF5I | GGATG | 3 cut(s) | 159, 1924, 1944 |
| BstFNI | CGCG | 1 cut(s) | 1082 |
| BstH2I | RGCGCY | 1 cut(s) | 1344 |
| BstHHI | GCGC | 1 cut(s) | 1343 |
| BstKTI | GATC | 9 cut(s) | 9, 66, 151, 743, 854, 911, 949, 1033, 1720 |
| BstMAI | GTCTC | 1 cut(s) | 1575 |
| BstMBI | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| BstMCI | CGRYCG | 1 cut(s) | 223 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 165, 840, 1948 |
| BstNI | CCWGG | 2 cut(s) | 172, 1936 |
| BstNSI | RCATGY | 2 cut(s) | 484, 1648 |
| BstSCI | CCNGG | 3 cut(s) | 170, 377, 1934 |
| BstSFI | CTRYAG | 1 cut(s) | 847 |
| BstUI | CGCG | 1 cut(s) | 1082 |
| BstV1I | GCAGC | 2 cut(s) | 369, 1233 |
| BstV2I | GAAGAC | 1 cut(s) | 232 |
| BstX2I | RGATCY | 3 cut(s) | 6, 851, 946 |
| BstYI | RGATCY | 3 cut(s) | 6, 851, 946 |
| BstZI | CGGCCG | 1 cut(s) | 220 |
| Bsu15I | ATCGAT | 1 cut(s) | 739 |
| BsuRI | GGCC | 4 cut(s) | 77, 222, 995, 1633 |
| BsuTUI | ATCGAT | 1 cut(s) | 739 |
| BtrI | CACGTC | 1 cut(s) | 1585 |
| BtsCI | GGATG | 3 cut(s) | 159, 1924, 1944 |
| BtsIMutI | CAGTG | 1 cut(s) | 151 |
| Cac8I | GCNNGC | 4 cut(s) | 123, 1165, 1339, 1768 |
| CfoI | GCGC | 1 cut(s) | 1343 |
| Cfr10I | RCCGGY | 1 cut(s) | 995 |
| Cfr13I | GGNCC | 2 cut(s) | 250, 993 |
| ClaI | ATCGAT | 1 cut(s) | 739 |
| Csp6I | GTAC | 7 cut(s) | 389, 704, 839, 1064, 1524, 1755, 1922 |
| CviQI | GTAC | 7 cut(s) | 389, 704, 839, 1064, 1524, 1755, 1922 |
| DdeI | CTNAG | 7 cut(s) | 34, 89, 164, 311, 708, 771, 1414 |
| DpnI | GATC | 9 cut(s) | 8, 65, 150, 742, 853, 910, 948, 1032, 1719 |
| DpnII | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| DraI | TTTAAA | 2 cut(s) | 130, 1395 |
| DriI | GACNNNNNGTC | 1 cut(s) | 256 |
| EaeI | YGGCCR | 1 cut(s) | 220 |
| EagI | CGGCCG | 1 cut(s) | 220 |
| Eam1104I | CTCTTC | 5 cut(s) | 85, 979, 1510, 1798, 1806 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 256 |
| EarI | CTCTTC | 5 cut(s) | 85, 979, 1510, 1798, 1806 |
| EclXI | CGGCCG | 1 cut(s) | 220 |
| Eco130I | CCWWGG | 1 cut(s) | 823 |
| Eco32I | GATATC | 1 cut(s) | 424 |
| Eco47I | GGWCC | 1 cut(s) | 250 |
| Eco47III | AGCGCT | 1 cut(s) | 1342 |
| Eco52I | CGGCCG | 1 cut(s) | 220 |
| Eco57I | CTGAAG | 1 cut(s) | 885 |
| Eco88I | CYCGRG | 1 cut(s) | 398 |
| EcoRI | GAATTC | 1 cut(s) | 495 |
| EcoRII | CCWGG | 2 cut(s) | 170, 1934 |
| EcoRV | GATATC | 1 cut(s) | 424 |
| EcoT14I | CCWWGG | 1 cut(s) | 823 |
| EcoT22I | ATGCAT | 2 cut(s) | 17, 1227 |
| ErhI | CCWWGG | 1 cut(s) | 823 |
| Esp3I | CGTCTC | 1 cut(s) | 1575 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1803, 1835 |
| FaqI | GGGAC | 2 cut(s) | 361, 817 |
| FauNDI | CATATG | 1 cut(s) | 940 |
| FbaI | TGATCA | 1 cut(s) | 63 |
| Fnu4HI | GCNGC | 4 cut(s) | 220, 383, 521, 1247 |
| FokI | GGATG | 3 cut(s) | 146, 1931, 1951 |
| Fsp4HI | GCNGC | 4 cut(s) | 220, 383, 521, 1247 |
| GlaI | GCGC | 1 cut(s) | 1342 |
| GluI | GCNGC | 4 cut(s) | 220, 383, 521, 1247 |
| GsuI | CTGGAG | 1 cut(s) | 239 |
| HaeII | RGCGCY | 1 cut(s) | 1344 |
| HaeIII | GGCC | 4 cut(s) | 77, 222, 995, 1633 |
| HapII | CCGG | 3 cut(s) | 379, 996, 1484 |
| HhaI | GCGC | 1 cut(s) | 1343 |
| Hin6I | GCGC | 1 cut(s) | 1341 |
| HinP1I | GCGC | 1 cut(s) | 1341 |
| HincII | GTYRAC | 2 cut(s) | 897, 1570 |
| HindII | GTYRAC | 2 cut(s) | 897, 1570 |
| HindIII | AAGCTT | 1 cut(s) | 1200 |
| HinfI | GANTC | 7 cut(s) | 179, 237, 503, 526, 1253, 1300, 1599 |
| HpaII | CCGG | 3 cut(s) | 379, 996, 1484 |
| HphI | GGTGA | 3 cut(s) | 431, 583, 647 |
| Hpy166II | GTNNAC | 4 cut(s) | 704, 897, 1570, 1650 |
| Hpy188I | TCNGA | 4 cut(s) | 136, 296, 904, 1683 |
| Hpy8I | GTNNAC | 4 cut(s) | 704, 897, 1570, 1650 |
| HpyAV | CCTTC | 3 cut(s) | 410, 604, 626 |
| HpyCH4III | ACNGT | 2 cut(s) | 51, 244 |
| HpyCH4IV | ACGT | 5 cut(s) | 1332, 1407, 1584, 1699, 1906 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 165, 840, 1948 |
| HpyF3I | CTNAG | 7 cut(s) | 34, 89, 164, 311, 708, 771, 1414 |
| HpySE526I | ACGT | 5 cut(s) | 1332, 1407, 1584, 1699, 1906 |
| HspAI | GCGC | 1 cut(s) | 1341 |
| KpnI | GGTACC | 1 cut(s) | 1527 |
| Ksp22I | TGATCA | 1 cut(s) | 63 |
| Kzo9I | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| LguI | GCTCTTC | 1 cut(s) | 1510 |
| LmnI | GCTCC | 2 cut(s) | 173, 1746 |
| Lsp1109I | GCAGC | 2 cut(s) | 369, 1233 |
| LweI | GCATC | 5 cut(s) | 168, 434, 1212, 1605, 1929 |
| MaeII | ACGT | 5 cut(s) | 1332, 1407, 1584, 1699, 1906 |
| MaeIII | GTNAC | 7 cut(s) | 238, 548, 1156, 1403, 1442, 1460, 1605 |
| MalI | GATC | 9 cut(s) | 8, 65, 150, 742, 853, 910, 948, 1032, 1719 |
| MboI | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| MfeI | CAATTG | 1 cut(s) | 807 |
| MflI | RGATCY | 3 cut(s) | 6, 851, 946 |
| MhlI | GDGCHC | 2 cut(s) | 1751, 1953 |
| MlyI | GAGTC | 1 cut(s) | 246 |
| MmeI | TCCRAC | 2 cut(s) | 210, 768 |
| Mph1103I | ATGCAT | 2 cut(s) | 17, 1227 |
| MroXI | GAANNNNTTC | 1 cut(s) | 407 |
| MseI | TTAA | 7 cut(s) | 129, 320, 621, 713, 929, 1394, 1966 |
| MslI | CAYNNNNRTG | 1 cut(s) | 20 |
| MspA1I | CMGCKG | 1 cut(s) | 1259 |
| MspI | CCGG | 3 cut(s) | 379, 996, 1484 |
| MspR9I | CCNGG | 3 cut(s) | 172, 379, 1936 |
| MunI | CAATTG | 1 cut(s) | 807 |
| Mva1269I | GAATGC | 1 cut(s) | 276 |
| MvaI | CCWGG | 2 cut(s) | 172, 1936 |
| MvnI | CGCG | 1 cut(s) | 1082 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 165, 840, 1948 |
| NciI | CCSGG | 1 cut(s) | 379 |
| NdeI | CATATG | 1 cut(s) | 940 |
| NdeII | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| NheI | GCTAGC | 1 cut(s) | 1337 |
| NlaIV | GGNNCC | 4 cut(s) | 994, 1136, 1525, 1933 |
| NmuCI | GTSAC | 3 cut(s) | 238, 1156, 1442 |
| NsiI | ATGCAT | 2 cut(s) | 17, 1227 |
| NspI | RCATGY | 2 cut(s) | 484, 1648 |
| NspV | TTCGAA | 1 cut(s) | 414 |
| PciI | ACATGT | 2 cut(s) | 480, 1644 |
| PciSI | GCTCTTC | 1 cut(s) | 1510 |
| PctI | GAATGC | 1 cut(s) | 276 |
| PdmI | GAANNNNTTC | 1 cut(s) | 407 |
| PfeI | GAWTC | 6 cut(s) | 179, 503, 526, 1253, 1300, 1599 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 271, 1550 |
| PfoI | TCCNGGA | 1 cut(s) | 170 |
| PkrI | GCNGC | 4 cut(s) | 221, 384, 522, 1248 |
| PleI | GAGTC | 1 cut(s) | 245 |
| PpsI | GAGTC | 1 cut(s) | 245 |
| Ppu21I | YACGTR | 1 cut(s) | 1907 |
| PscI | ACATGT | 2 cut(s) | 480, 1644 |
| Psp1406I | AACGTT | 1 cut(s) | 1407 |
| Psp6I | CCWGG | 2 cut(s) | 170, 1934 |
| PspGI | CCWGG | 2 cut(s) | 170, 1934 |
| PspN4I | GGNNCC | 4 cut(s) | 994, 1136, 1525, 1933 |
| PspPI | GGNCC | 2 cut(s) | 250, 993 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 991, 1023 |
| PsuI | RGATCY | 3 cut(s) | 6, 851, 946 |
| PvuII | CAGCTG | 1 cut(s) | 1259 |
| RsaI | GTAC | 7 cut(s) | 390, 705, 840, 1065, 1525, 1756, 1923 |
| RsaNI | GTAC | 7 cut(s) | 389, 704, 839, 1064, 1524, 1755, 1922 |
| RseI | CAYNNNNRTG | 1 cut(s) | 20 |
| SapI | GCTCTTC | 1 cut(s) | 1510 |
| SaqAI | TTAA | 7 cut(s) | 129, 320, 621, 713, 929, 1394, 1966 |
| SatI | GCNGC | 4 cut(s) | 220, 383, 521, 1247 |
| Sau3AI | GATC | 9 cut(s) | 6, 63, 148, 740, 851, 908, 946, 1030, 1717 |
| Sau96I | GGNCC | 2 cut(s) | 250, 993 |
| ScaI | AGTACT | 2 cut(s) | 390, 1065 |
| SchI | GAGTC | 1 cut(s) | 246 |
| ScrFI | CCNGG | 3 cut(s) | 172, 379, 1936 |
| SduI | GDGCHC | 2 cut(s) | 1751, 1953 |
| SfaNI | GCATC | 5 cut(s) | 168, 434, 1212, 1605, 1929 |
| SfcI | CTRYAG | 1 cut(s) | 847 |
| SfuI | TTCGAA | 1 cut(s) | 414 |
| SinI | GGWCC | 1 cut(s) | 250 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 20 |
| SmlI | CTYRAG | 2 cut(s) | 301, 357 |
| SmoI | CTYRAG | 2 cut(s) | 301, 357 |
| SpeI | ACTAGT | 2 cut(s) | 1153, 1751 |
| SsiI | CCGC | 2 cut(s) | 219, 520 |
| SspI | AATATT | 1 cut(s) | 1321 |
| StyD4I | CCNGG | 3 cut(s) | 170, 377, 1934 |
| StyI | CCWWGG | 1 cut(s) | 823 |
| TaaI | ACNGT | 2 cut(s) | 51, 244 |
| TaiI | ACGT | 5 cut(s) | 1335, 1410, 1587, 1702, 1909 |
| TaqI | TCGA | 6 cut(s) | 370, 414, 739, 1029, 1514, 1662 |
| TatI | WGTACW | 4 cut(s) | 388, 1063, 1754, 1921 |
| TauI | GCSGC | 2 cut(s) | 222, 523 |
| TfiI | GAWTC | 6 cut(s) | 179, 503, 526, 1253, 1300, 1599 |
| Tru1I | TTAA | 7 cut(s) | 129, 320, 621, 713, 929, 1394, 1966 |
| Tru9I | TTAA | 7 cut(s) | 129, 320, 621, 713, 929, 1394, 1966 |
| TscAI | CASTG | 1 cut(s) | 151 |
| TseFI | GTSAC | 3 cut(s) | 238, 1156, 1442 |
| TseI | GCWGC | 2 cut(s) | 382, 1246 |
| Tsp45I | GTSAC | 3 cut(s) | 238, 1156, 1442 |
| TspDTI | ATGAA | 4 cut(s) | 912, 1090, 1373, 1809 |
| TspGWI | ACGGA | 2 cut(s) | 489, 1593 |
| TspRI | CASTG | 1 cut(s) | 151 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 271, 1550 |
| VpaK11BI | GGWCC | 1 cut(s) | 250 |
| XapI | RAATTY | 4 cut(s) | 495, 586, 690, 982 |
| XbaI | TCTAGA | 1 cut(s) | 1234 |
| XceI | RCATGY | 2 cut(s) | 484, 1648 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 268 |
| XmaJI | CCTAGG | 1 cut(s) | 823 |
| XmnI | GAANNNNTTC | 1 cut(s) | 407 |
| ZrmI | AGTACT | 2 cut(s) | 390, 1065 |
| Zsp2I | ATGCAT | 2 cut(s) | 17, 1227 |