MD08G1107900.v1.1

Wall-associated receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000052
Physical Location & Seq
Reverse (-)
9481554 .. 9485041
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1107900.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGAGCAAACAAAAAGTGCTTCTGCAACCTATCATCCTCCTAATAGTAATAGAGGTACTATTAGCAGCAGCCACTTCAGCCCAAACACAATTAGCCTCTCCACCTCTTCCCTCTCCTCCTCCTCCCAGTGAAGTAGAAGTCAAGCCGGGTTGTCAGGACAAATGTGGGAATGTTAGCATCCCATATCCATTTGGTACAAAAAAGGGCTGTTACTTAAATGAGGATTTCCTCATTACTTGTAATAGCACACATTATGAACCCCCAAAACCATTTTTTAAGAAGAGTTATTCGACTGAAGTCACAAACATATCCGTGGATGGGAAGCTTTACATTACCCTATACCCAGCCGTAATATGTTACGACAAATCAGGCCAGCTGACTTACGAATTCACCAGTTCTGTCAATCTTTCCAGTTTCTTCATATCAAACACCGACAATGTGTATGTCGCAGTTGGTTGTGACACCTATTCGTACATTTACGGCTACAAAAATGAAAAATACGTGTATAGTGGTGGGTGCATTTCATATTGTGCCGGCATTGATGACTACGTTGTCAATGGTTCCTGCAATGGTTTCGGCTGTTGTCAAACAAGCTTTGCTCAAGGAATAAATTATTTCGAAGTAAGTGCAATGAGTTTTAAAAATCATACGGATGTGTACTACTTTAATCCTTGCAGTTTTTCCTTCGTTGTCCAAGACGGCAAGTTCAACTTCTTCTCCAATATGTTGGGGTCGGATTATTTAAAAAATGCATCCCTTCCGGTGGTACTCGATTGGTCAGTTGGAAGTGAGAACTGCACTAACGTTGTTAACAAGAAAAATCACCCATGTAAGGGCAACGCATCGTGTTATGATGTCGAGAATGGATCCGGGTATCGCTGCAAGTGCAATGACGGTTTCCAAGGGAATCCCTACCTAAATGGCTGCCATGACATTGATGAATGTAAGGTTCCAGAACTCAATACATGCATGCAAAAATGTATAAATACAGAGGGAAGCCATACTTGTTCTTGCTACAAGGGGTACCATGGTGATGGCAGAAAAGACGGAGAAGGTTGCACCCCTGATCGGACCTCTGTTATCCCAATTGTCGTTGGTCTTGGTGCGGGCGTGGGACTATTGTTACTGCTTATCAGCGCATGGTGGATACACAAAGTCCTGAAGAAAAGGAAGAACATAAAACGCAAGCAAGTTTTCTTCAAACGAAATGGTGGTTTATTATTAGAGCAACAATTATCTTCAGGTGAAGTTAATGTGGAGAAAATCAAGTTGTTCAAGTCGAAGGAGTTGGAGAAGTCTACCGACAATTTCAACGTCGATAGAATTCTTGGGCATGGAGGCCAAGGTACCGTGTACAAGGGCATGTTGGCTGATGGTAAAATTGTTGCCGTAAAAAAGTCCACAATTATTGATGGAAGCCAACTTTCAGATTTCATCAATGAGGTTGTAATTCTTTCACAAATCAACCACAGACATGTTGTTCAACTATTGGGTTGCTGCTTGGAGACTGAAGTTCCGCTTTTGGTTTATGAATTTATTCCGAATGGAACACTTTCCCAGTATATCCATGAGCAGAAAGAGGAGTTTCCACTTACATGGGAAATTCGCTTACAAATTGCCACAGAAATTGCAGGAGCCCTTTTCTATTTACACGCCTCAGCTGCCCTTCCCATTTATCACAGGGACATCAAGTCTACCAACATACTCTTGGATGCAAAATACAGAGCAAAAGTTGCCGATTTTGGAACTTCAAGATCAGTTGCCATTGACCAAACTCACCTTACCACACTTGTACATGGCACATTTGGTTACTTGGACCCGGAATACTTCCAGTCAAACCATTTTACGGATAAAAGTGATGTGTATAGCTTTGGAGTGGTCCTTGTTGAGCTATTAACGGGACAAAAACCAATTTCTTTTAGCCGGTCACAAGAACAAGGCAGAAGTCTTGCTACATACTTCATTATTTCGATGCAGGAAGATCGTCTGTTTGACATTCTTGATGCTCAAGTTGTGAAGGGAGGGTCTAAAGCAGATATCGCAATAGTTGCTAACCTTGCAAGGAGGTGCTTGAATTTAAGTGGACGAAAACGCCCAACAATGAGAGAGGTCACGGCAGAGCTGGAGGGGGTTCAAATGGCCGGAAAGGCTACTTCTCATGGTGGAAAGAATTACGAAGAGGTTGAATATGTGAGGACTGAATCAGTTGAGCCATGGGATGTTATTTCAAGCTCAACTGATACAGGGCCTGCAGTGGATGGTGGTGCTGCTTCATCACAAATGCATGAAATCCCACTCTTACCTTTCAAGTCACGGTGA

Protein Analysis

773

Amino Acids

85.33

Weight (kDa)

6.33

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 51 - 108 9.7e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 311 - 348 1.4e-06 Calcium-binding EGF domain
Pkinase PF00069 437 - 705 4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 708 8.1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
Acc65I GGTACC 2 cut(s) 1023, 1346
AccB1I GGYRCC 2 cut(s) 1023, 1346
AccI GTMKAC 2 cut(s) 1298, 1694
AciI CCGC 2 cut(s) 1106, 1517
AclI AACGTT 1 cut(s) 804
AclWI GGATC 2 cut(s) 861, 874
AcoI YGGCCR 1 cut(s) 2139
AcsI RAATTY 5 cut(s) 386, 1323, 1532, 1602, 2074
AcuI CTGAAG 5 cut(s) 60, 315, 1181, 1224, 1530
AfaI GTAC 9 cut(s) 57, 196, 473, 659, 768, 1025, 1348, 1355, 1794
AfiI CCNNNNNNNGG 6 cut(s) 318, 763, 832, 833, 1068, 1846
AflIII ACRYGT 2 cut(s) 501, 1474
AhdI GACNNNNNGTC 1 cut(s) 1690
AluBI AGCT 8 cut(s) 325, 376, 594, 1661, 1869, 1891, 2122, 2232
AluI AGCT 8 cut(s) 325, 376, 594, 1661, 1869, 1891, 2122, 2232
Alw26I GTCTC 1 cut(s) 1499
AlwI GGATC 2 cut(s) 861, 874
AlwNI CAGNNNCTG 1 cut(s) 2249
AoxI GGCC 4 cut(s) 370, 1339, 2139, 2246
ApeKI GCWGC 7 cut(s) 65, 68, 879, 924, 1497, 1661, 2267
ApoI RAATTY 5 cut(s) 386, 1323, 1532, 1602, 2074
Asp700I GAANNNNTTC 2 cut(s) 1536, 1552
Asp718I GGTACC 2 cut(s) 1023, 1346
AspLEI GCGC 1 cut(s) 1139
AspS9I GGNCC 4 cut(s) 1071, 1816, 1879, 2246
AsuC2I CCSGG 3 cut(s) 147, 871, 1820
AsuHPI GGTGA 5 cut(s) 382, 815, 1043, 1256, 1769
AsuII TTCGAA 1 cut(s) 618
AvaII GGWCC 3 cut(s) 1071, 1816, 1879
BaeI ACNNNNGTAYC 2 cut(s) 1015, 1048
BamHI GGATCC 1 cut(s) 866
BanI GGYRCC 2 cut(s) 1023, 1346
BanII GRGCYC 1 cut(s) 1639
BarI GAAGNNNNNNTAC 2 cut(s) 1936, 1968
BbvCI CCTCAGC 1 cut(s) 1657
BbvI GCAGC 7 cut(s) 77, 80, 866, 911, 1484, 1648, 2254
BccI CCATC 5 cut(s) 311, 1028, 1367, 1406, 2252
BceAI ACGGC 5 cut(s) 332, 496, 715, 1373, 2130
BcgI CGANNNNNNTGC 6 cut(s) 556, 590, 608, 642, 1964, 1998
BciVI GTATCC 1 cut(s) 1140
BcnI CCSGG 3 cut(s) 147, 871, 1820
BcoDI GTCTC 1 cut(s) 1499
BfmI CTRYAG 1 cut(s) 2250
BfuI GTATCC 1 cut(s) 1140
BglI GCCNNNNNGGC 1 cut(s) 2147
BisI GCNGC 7 cut(s) 66, 69, 880, 925, 1498, 1662, 2268
BlsI GCNGC 7 cut(s) 67, 70, 881, 926, 1499, 1663, 2269
Bme1390I CCNGG 3 cut(s) 147, 871, 1820
Bme18I GGWCC 3 cut(s) 1071, 1816, 1879
BmeRI GACNNNNNGTC 1 cut(s) 1690
BmgT120I GGNCC 4 cut(s) 1071, 1816, 1879, 2246
BmiI GGNNCC 7 cut(s) 562, 868, 951, 1025, 1348, 1636, 1818
BmrFI CCNGG 3 cut(s) 147, 871, 1820
BmrI ACTGGG 2 cut(s) 120, 1552
BmsI GCATC 6 cut(s) 186, 761, 851, 1702, 1962, 1993
BmuI ACTGGG 2 cut(s) 120, 1552
BoxI GACNNNNGTC 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 2144
Bpu10I CCTNAGC 1 cut(s) 1657
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 585, 1992
BpuMI CCSGG 3 cut(s) 147, 871, 1820
BsaAI YACGTR 1 cut(s) 502
BsaJI CCNNGG 5 cut(s) 312, 901, 1027, 1342, 2213
BsaWI WCCGGW 1 cut(s) 760
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bsc4I CCNNNNNNNGG 6 cut(s) 318, 763, 832, 833, 1068, 1846
Bse118I RCCGGY 2 cut(s) 533, 1923
Bse1I ACTGG 5 cut(s) 126, 393, 411, 1558, 1831
Bse3DI GCAATG 3 cut(s) 574, 636, 895
BseDI CCNNGG 5 cut(s) 312, 901, 1027, 1342, 2213
BseGI GGATG 8 cut(s) 33, 177, 322, 658, 752, 1717, 2224, 2263
BseLI CCNNNNNNNGG 6 cut(s) 318, 763, 832, 833, 1068, 1846
BseMI GCAATG 3 cut(s) 574, 636, 895
BseMII CTCAG 1 cut(s) 1671
BseNI ACTGG 5 cut(s) 126, 393, 411, 1558, 1831
BseRI GAGGAG 4 cut(s) 105, 108, 111, 1595
BseXI GCAGC 7 cut(s) 77, 80, 866, 911, 1484, 1648, 2254
BseYI CCCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 781
BshFI GGCC 4 cut(s) 372, 1341, 2141, 2248
BshNI GGYRCC 2 cut(s) 1023, 1346
BsiSI CCGG 7 cut(s) 146, 534, 761, 870, 1820, 1924, 2142
BslFI GGGAC 3 cut(s) 1128, 1697, 1914
BslI CCNNNNNNNGG 6 cut(s) 318, 763, 832, 833, 1068, 1846
BsmAI GTCTC 1 cut(s) 1499
BsmFI GGGAC 3 cut(s) 1128, 1697, 1914
BsnI GGCC 4 cut(s) 372, 1341, 2141, 2248
Bsp119I TTCGAA 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 1639
Bsp1407I TGTACA 2 cut(s) 1353, 1792
Bsp143I GATC 4 cut(s) 866, 1066, 1754, 1981
Bsp19I CCATGG 2 cut(s) 1027, 2213
BspACI CCGC 2 cut(s) 1106, 1517
BspANI GGCC 4 cut(s) 372, 1341, 2141, 2248
BspCNI CTCAG 1 cut(s) 1670
BspLI GGNNCC 7 cut(s) 562, 868, 951, 1025, 1348, 1636, 1818
BspMAI CTGCAG 1 cut(s) 2254
BspPI GGATC 2 cut(s) 861, 874
BspT104I TTCGAA 1 cut(s) 618
BspT107I GGYRCC 2 cut(s) 1023, 1346
BsrDI GCAATG 3 cut(s) 574, 636, 895
BsrFI RCCGGY 2 cut(s) 533, 1923
BsrGI TGTACA 2 cut(s) 1353, 1792
BsrI ACTGG 5 cut(s) 126, 393, 411, 1558, 1831
BssAI RCCGGY 2 cut(s) 533, 1923
BssECI CCNNGG 5 cut(s) 312, 901, 1027, 1342, 2213
BssMI GATC 4 cut(s) 866, 1066, 1754, 1981
BssT1I CCWWGG 4 cut(s) 901, 1027, 1342, 2213
Bst4CI ACNGT 3 cut(s) 896, 1351, 2316
Bst6I CTCTTC 3 cut(s) 111, 275, 2172
BstAPI GCANNNNNTGC 2 cut(s) 1733, 2048
BstAUI TGTACA 2 cut(s) 1353, 1792
BstBAI YACGTR 1 cut(s) 502
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 6 cut(s) 374, 535, 971, 1108, 1187, 2250
BstDEI CTNAG 1 cut(s) 1657
BstDSI CCRYGG 3 cut(s) 312, 1027, 2213
BstF5I GGATG 8 cut(s) 33, 177, 322, 658, 752, 1717, 2224, 2263
BstHHI GCGC 1 cut(s) 1139
BstKTI GATC 4 cut(s) 869, 1069, 1757, 1984
BstMAI GTCTC 1 cut(s) 1499
BstMBI GATC 4 cut(s) 866, 1066, 1754, 1981
BstMWI GCNNNNNNNGC 8 cut(s) 77, 885, 1661, 1733, 2039, 2048, 2057, 2147
BstNSI RCATGY 4 cut(s) 969, 973, 1366, 1478
BstPAI GACNNNNGTC 1 cut(s) 296
BstSCI CCNGG 3 cut(s) 145, 869, 1818
BstSFI CTRYAG 1 cut(s) 2250
BstV1I GCAGC 7 cut(s) 77, 80, 866, 911, 1484, 1648, 2254
BstX2I RGATCY 1 cut(s) 866
BstXI CCANNNNNNTGG 3 cut(s) 727, 1034, 1596
BstYI RGATCY 1 cut(s) 866
BsuI GTATCC 1 cut(s) 1140
BsuRI GGCC 4 cut(s) 372, 1341, 2141, 2248
BtgI CCRYGG 3 cut(s) 312, 1027, 2213
BtsCI GGATG 8 cut(s) 33, 177, 322, 658, 752, 1717, 2224, 2263
BtsI GCAGTG 1 cut(s) 2259
BtsIMutI CAGTG 2 cut(s) 133, 2259
Cac8I GCNNGC 6 cut(s) 374, 535, 971, 1108, 1187, 2250
CaiI CAGNNNCTG 1 cut(s) 2249
CfoI GCGC 1 cut(s) 1139
Cfr10I RCCGGY 2 cut(s) 533, 1923
Cfr13I GGNCC 4 cut(s) 1071, 1816, 1879, 2246
Csp6I GTAC 9 cut(s) 56, 195, 472, 658, 767, 1024, 1347, 1354, 1793
CspCI CAANNNNNGTGG 4 cut(s) 1390, 1425, 1609, 1644
CviQI GTAC 9 cut(s) 56, 195, 472, 658, 767, 1024, 1347, 1354, 1793
DdeI CTNAG 1 cut(s) 1657
DpnI GATC 4 cut(s) 868, 1068, 1756, 1983
DpnII GATC 4 cut(s) 866, 1066, 1754, 1981
DraI TTTAAA 2 cut(s) 640, 744
DrdI GACNNNNNNGTC 1 cut(s) 551
DriI GACNNNNNGTC 1 cut(s) 1690
DseDI GACNNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 1 cut(s) 2139
Eam1104I CTCTTC 3 cut(s) 111, 275, 2172
Eam1105I GACNNNNNGTC 1 cut(s) 1690
EarI CTCTTC 3 cut(s) 111, 275, 2172
Eco130I CCWWGG 4 cut(s) 901, 1027, 1342, 2213
Eco24I GRGCYC 1 cut(s) 1639
Eco32I GATATC 1 cut(s) 2038
Eco47I GGWCC 3 cut(s) 1071, 1816, 1879
Eco57I CTGAAG 5 cut(s) 60, 315, 1181, 1224, 1530
EcoO109I RGGNCCY 1 cut(s) 2246
EcoRI GAATTC 2 cut(s) 386, 1323
EcoRV GATATC 1 cut(s) 2038
EcoT14I CCWWGG 4 cut(s) 901, 1027, 1342, 2213
EcoT22I ATGCAT 3 cut(s) 754, 971, 2286
EcoT38I GRGCYC 1 cut(s) 1639
ErhI CCWWGG 4 cut(s) 901, 1027, 1342, 2213
FalI AAGNNNNNCTT 6 cut(s) 695, 727, 988, 1020, 1503, 1535
FaqI GGGAC 3 cut(s) 1128, 1697, 1914
FauI CCCGC 1 cut(s) 1099
FblI GTMKAC 2 cut(s) 1298, 1694
Fnu4HI GCNGC 7 cut(s) 66, 69, 880, 925, 1498, 1662, 2268
FokI GGATG 8 cut(s) 20, 164, 329, 665, 739, 1724, 2231, 2270
FriOI GRGCYC 1 cut(s) 1639
Fsp4HI GCNGC 7 cut(s) 66, 69, 880, 925, 1498, 1662, 2268
GlaI GCGC 1 cut(s) 1138
GluI GCNGC 7 cut(s) 66, 69, 880, 925, 1498, 1662, 2268
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 2144
HaeIII GGCC 4 cut(s) 372, 1341, 2141, 2248
HapII CCGG 7 cut(s) 146, 534, 761, 870, 1820, 1924, 2142
HhaI GCGC 1 cut(s) 1139
Hin6I GCGC 1 cut(s) 1137
HinP1I GCGC 1 cut(s) 1137
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 2 cut(s) 323, 592
HinfI GANTC 2 cut(s) 907, 2201
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 7 cut(s) 146, 534, 761, 870, 1820, 1924, 2142
HphI GGTGA 5 cut(s) 382, 815, 1043, 1256, 1769
Hpy166II GTNNAC 7 cut(s) 658, 811, 1299, 1354, 1401, 1695, 2084
Hpy188I TCNGA 4 cut(s) 736, 1071, 1429, 1542
Hpy188III TCNNGA 6 cut(s) 155, 859, 953, 1159, 1752, 2000
Hpy8I GTNNAC 7 cut(s) 658, 811, 1299, 1354, 1401, 1695, 2084
Hpy99I CGWCG 1 cut(s) 1319
HpyAV CCTTC 6 cut(s) 694, 767, 1046, 1276, 1676, 2011
HpyCH4III ACNGT 3 cut(s) 896, 1351, 2316
HpyCH4IV ACGT 4 cut(s) 501, 549, 804, 1314
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 885, 1661, 1733, 2039, 2048, 2057, 2147
HpyF3I CTNAG 1 cut(s) 1657
HpySE526I ACGT 4 cut(s) 501, 549, 804, 1314
HspAI GCGC 1 cut(s) 1137
KpnI GGTACC 2 cut(s) 1027, 1350
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 4 cut(s) 866, 1066, 1754, 1981
LmnI GCTCC 1 cut(s) 1634
Lsp1109I GCAGC 7 cut(s) 77, 80, 866, 911, 1484, 1648, 2254
LweI GCATC 6 cut(s) 186, 761, 851, 1702, 1962, 1993
MaeII ACGT 4 cut(s) 501, 549, 804, 1314
MaeIII GTNAC 9 cut(s) 209, 298, 356, 458, 1122, 1808, 1926, 2110, 2310
MalI GATC 4 cut(s) 868, 1068, 1756, 1983
MboI GATC 4 cut(s) 866, 1066, 1754, 1981
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 1 cut(s) 866
MhlI GDGCHC 1 cut(s) 1639
MmeI TCCRAC 3 cut(s) 714, 763, 1269
Mph1103I ATGCAT 3 cut(s) 754, 971, 2286
MroNI GCCGGC 1 cut(s) 533
MroXI GAANNNNTTC 2 cut(s) 1536, 1552
MseI TTAA 9 cut(s) 215, 276, 639, 666, 743, 810, 1251, 1895, 2078
MslI CAYNNNNRTG 5 cut(s) 311, 651, 1032, 1473, 1594
MspA1I CMGCKG 2 cut(s) 376, 1661
MspI CCGG 7 cut(s) 146, 534, 761, 870, 1820, 1924, 2142
MspR9I CCNGG 3 cut(s) 147, 871, 1820
MunI CAATTG 1 cut(s) 1086
MwoI GCNNNNNNNGC 8 cut(s) 77, 885, 1661, 1733, 2039, 2048, 2057, 2147
NaeI GCCGGC 1 cut(s) 535
NciI CCSGG 3 cut(s) 147, 871, 1820
NcoI CCATGG 2 cut(s) 1027, 2213
NdeII GATC 4 cut(s) 866, 1066, 1754, 1981
NgoMIV GCCGGC 1 cut(s) 533
NlaIV GGNNCC 7 cut(s) 562, 868, 951, 1025, 1348, 1636, 1818
NmuCI GTSAC 5 cut(s) 298, 458, 1926, 2110, 2310
NsiI ATGCAT 3 cut(s) 754, 971, 2286
NspI RCATGY 4 cut(s) 969, 973, 1366, 1478
NspV TTCGAA 1 cut(s) 618
PaeI GCATGC 1 cut(s) 973
PciI ACATGT 1 cut(s) 1474
PdiI GCCGGC 1 cut(s) 535
PdmI GAANNNNTTC 2 cut(s) 1536, 1552
PfeI GAWTC 2 cut(s) 907, 2201
PkrI GCNGC 7 cut(s) 67, 70, 881, 926, 1499, 1663, 2269
Ppu21I YACGTR 1 cut(s) 502
PscI ACATGT 1 cut(s) 1474
PshAI GACNNNNGTC 1 cut(s) 296
Psp1406I AACGTT 1 cut(s) 804
PspFI CCCAGC 1 cut(s) 343
PspN4I GGNNCC 7 cut(s) 562, 868, 951, 1025, 1348, 1636, 1818
PspPI GGNCC 4 cut(s) 1071, 1816, 1879, 2246
PstI CTGCAG 1 cut(s) 2254
PstNI CAGNNNCTG 1 cut(s) 2249
PsuI RGATCY 1 cut(s) 866
PvuII CAGCTG 2 cut(s) 376, 1661
RsaI GTAC 9 cut(s) 57, 196, 473, 659, 768, 1025, 1348, 1355, 1794
RsaNI GTAC 9 cut(s) 56, 195, 472, 658, 767, 1024, 1347, 1354, 1793
RseI CAYNNNNRTG 5 cut(s) 311, 651, 1032, 1473, 1594
SaqAI TTAA 9 cut(s) 215, 276, 639, 666, 743, 810, 1251, 1895, 2078
SatI GCNGC 7 cut(s) 66, 69, 880, 925, 1498, 1662, 2268
Sau3AI GATC 4 cut(s) 866, 1066, 1754, 1981
Sau96I GGNCC 4 cut(s) 1071, 1816, 1879, 2246
ScrFI CCNGG 3 cut(s) 147, 871, 1820
SduI GDGCHC 1 cut(s) 1639
SfaNI GCATC 6 cut(s) 186, 761, 851, 1702, 1962, 1993
SfcI CTRYAG 1 cut(s) 2250
SfuI TTCGAA 1 cut(s) 618
SinI GGWCC 3 cut(s) 1071, 1816, 1879
SmiMI CAYNNNNRTG 5 cut(s) 311, 651, 1032, 1473, 1594
SmlI CTYRAG 2 cut(s) 600, 2007
SmoI CTYRAG 2 cut(s) 600, 2007
SphI GCATGC 1 cut(s) 973
SsiI CCGC 2 cut(s) 1106, 1517
StyD4I CCNGG 3 cut(s) 145, 869, 1818
StyI CCWWGG 4 cut(s) 901, 1027, 1342, 2213
TaaI ACNGT 3 cut(s) 896, 1351, 2316
TaiI ACGT 4 cut(s) 504, 552, 807, 1317
TaqI TCGA 7 cut(s) 290, 618, 771, 858, 1280, 1317, 1970
TatI WGTACW 3 cut(s) 657, 1353, 1792
TfiI GAWTC 2 cut(s) 907, 2201
Tru1I TTAA 9 cut(s) 215, 276, 639, 666, 743, 810, 1251, 1895, 2078
Tru9I TTAA 9 cut(s) 215, 276, 639, 666, 743, 810, 1251, 1895, 2078
TscAI CASTG 2 cut(s) 133, 2259
TseFI GTSAC 5 cut(s) 298, 458, 1926, 2110, 2310
TseI GCWGC 7 cut(s) 65, 68, 879, 924, 1497, 1661, 2267
Tsp45I GTSAC 5 cut(s) 298, 458, 1926, 2110, 2310
TspGWI ACGGA 4 cut(s) 301, 665, 1062, 1862
TspRI CASTG 2 cut(s) 133, 2259
VpaK11BI GGWCC 3 cut(s) 1071, 1816, 1879
XapI RAATTY 5 cut(s) 386, 1323, 1532, 1602, 2074
XceI RCATGY 4 cut(s) 969, 973, 1366, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 1705
XmiI GTMKAC 2 cut(s) 1298, 1694
XmnI GAANNNNTTC 2 cut(s) 1536, 1552
Zsp2I ATGCAT 3 cut(s) 754, 971, 2286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.