Rw2G040810

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000052
Physical Location & Seq
Reverse (-)
66414223 .. 66418516
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040810.1

Sequence Viewer

Length: 2259 bp
ATGGTTGCCCAGTTGCTATTTCATATTACTCTGTTACTGTGGTCTATGAGTAGTACTGAAGTACTAGTTGTAGCATCAGAAGCACCCATTGTAGCAACAGAAGCACCCATAGCAAAGCCTAATTGTCCATCGCATTGTGGAGGCATTGAAATCCCATACCCTTTTGGGATAGGAGCAGGTTGTTACATTCATGACGACTGGTTTCAAGTATACTGTGACAACTCCACTGGCTCTCCTACACCTTTCTTGAACGGCACCAATCTGGAGGTACTAGATATTTCTGTTGAAGGCACTCTAAAGGTCAGAAACCCAATCACCTTCTCTAATTGCAGTGATAAGCCAAATCGTCAAGCAGTCAACTTGGAGGGAAGCCCTTTTATGTTCTCCCAGAAGAACATGTTCACTGCAGTAGGTTGCGGAGTTATGGCCACAATCACCTCAAACTCAAATGGCGTTACCATTTCAGCCGGGTGCAGGTCCGAATGTTATGATTATACTGTTAGTAAAAATAGTAGCAGCCATAATACCTGCAATGGTGTGGATTGCTGCCAGACTGTCATCCCTCGTTCCCTCTCTGCCTTCAATACTAGTTTCCAGCGGGCTAATGATAATTACACAAATAGTTCATGTAATTATGCATTTTTGGTTGATCGTGACTGGTTTGAGTCATTTGCCAAAAATTCAACGAATATTTCTGCCACCAGTGATATGGACCAAGTCCCCGTGGAGCTAAAATGGAACTTGTACCATTCAACAACTGATGTTTTTGGGACCTTTATAGAAACAAATGCATCGATGTGGTATGCTGATGACCTAGATGGTCATTGCGAGACTTACAACGACAGCTCTTCATCATATGAATCCTTAAGGCTTGAATGTTCCTGTGGAGGGGGCTCTAAGGGAAATCCATATCTTCTTCAAGGATGCAGAGATATCAATGAATGTGAGGATCCGGAAATCAGAGCTTGTTCTGCAGGGTCCGTATGTGTCAATCAGCCTGGTGGATGGGGATGCAACCCCCCAGAAACCAGCAAGTCGAGGGTTAAAGTAATCTTTATAGGTATCGGCAGTGGTTTTGGACTGATAGTTCTACTAATTGGTGCATGGTGGTTGTACAAACTTTGGAAGAAAAGGAAAAACATTAAACTCAAGAAGAAGTTCTTTAAACAAAATGGGGGACTATTACTAGAGCAGCAACTATCTTCCGGTGAAGTTAATGTTGAGAAAATTAAGCTGTTCAATTCAAAGGAATTAGAAAAAGCCACAGACCGGTTTAATGTAGATAGAATACTCGGCCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGGCCGATGGAAGAATTGTTGCTGTAAAGAAGTCTAAAATAGTTGATGGAGGCGAAGTGGGACAATTCATTAATGAAATTGTCATTCTTTCACAAATTAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACCGAAGTTCCACTTTTGGTTTACGAATTCATACCCAAAGGGACTATCTACCAGTATCTCCATGATCAGAATGAAGAGTTTCCACTCACATGGGAAATGCGCTTAAGGATTTCTGCAGAAGTTGCCGGAGCTCTTTCATACTTACACTCTGCAGCTGCTTTCCCAATTTACCACCGTGATATCAAGTCTACAAACATACTATTAGATGATAAATACAGAGCAAAAGTTGCAGACTTTGGGACTTCAAGATCAGTTTCCATAGACCAAACACACCTAACCACATTTGTACATGGTACATTTGGTTACTTGGATCCGGAGTACTTCCAATCAAGTCAATTTACAGACAAAAGTGACGTGTATAGCTTTGGAGTGGTCCTTGTCGAGCTCTTGACTGGAGAAAAACCAGTTTCTCTGACTAGGTCACAAGAAGCCAGAGGCCTTGTTTCATATTTCAATCTCTCACTGGAGTCGAATAATTTGTTTGATATTATTGATGCTCGAGTTAAGGTGGAGGGTGTAACAGGAGATATTTTGACAGTAGCTAATCTTGCAAAAAGATGTCTGGATATGAATGGGAAGAGACGACCTACCATGAAAGAAGTTGCAATGGAGTTGGAGGTGATTCATAAATCAGTGAAATCGTCAGATATACAACAAAATCATGAAGAGGTTGAGTATGTCCGAAATGAAGTAACTTGTCTTTGGGATGTTGCTTCTACATCAACCAGGTCAGCTTTGGATGGTGGCACTGCTTCATCAATTGATTCACTACCACTAATGTCCAACTGA

Protein Analysis

752

Amino Acids

83.07

Weight (kDa)

5.42

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 5e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 425 - 691 1.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 427 - 695 1.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 167, 465, 536
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 2 cut(s) 210, 1658
AccIII TCCGGA 2 cut(s) 952, 1783
AciI CCGC 2 cut(s) 417, 598
AclWI GGATC 4 cut(s) 944, 957, 1775, 1788
AcoI YGGCCR 3 cut(s) 426, 1294, 1332
AcsI RAATTY 2 cut(s) 679, 1496
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 9 cut(s) 55, 63, 270, 746, 1115, 1312, 1758, 1765, 1790
AfiI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
AflII CTTAAG 2 cut(s) 865, 1573
AflIII ACRYGT 2 cut(s) 396, 1824
AgeI ACCGGT 1 cut(s) 1269
AhlI ACTAGT 2 cut(s) 64, 587
AjiI CACGTC 1 cut(s) 1825
AjnI CCWGG 2 cut(s) 997, 2195
AloI GAACNNNNNNTCC 2 cut(s) 1071, 1103
Alw21I GWGCWC 2 cut(s) 1603, 1857
Alw26I GTCTC 3 cut(s) 824, 1463, 2044
AlwI GGATC 4 cut(s) 944, 957, 1775, 1788
Ama87I CYCGRG 1 cut(s) 1968
Aor13HI TCCGGA 2 cut(s) 952, 1783
AoxI GGCC 5 cut(s) 426, 1294, 1303, 1332, 1906
ApeKI GCWGC 5 cut(s) 516, 546, 1192, 1622, 1625
ApoI RAATTY 2 cut(s) 679, 1496
AseI ATTAAT 1 cut(s) 1401
AsiGI ACCGGT 1 cut(s) 1269
Asp700I GAANNNNTTC 3 cut(s) 398, 1157, 1548
AspLEI GCGC 1 cut(s) 1572
AspS9I GGNCC 5 cut(s) 477, 712, 771, 978, 1843
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 4 cut(s) 307, 427, 1220, 2101
AvaI CYCGRG 1 cut(s) 1968
AvaII GGWCC 5 cut(s) 477, 712, 771, 978, 1843
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 2 cut(s) 949, 1780
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 3 cut(s) 896, 1603, 1857
Bbv12I GWGCWC 2 cut(s) 1603, 1857
BbvI GCAGC 5 cut(s) 528, 533, 1204, 1612, 1634
BccI CCATC 6 cut(s) 136, 812, 999, 1331, 1370, 2204
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 2 cut(s) 999, 2197
BclI TGATCA 1 cut(s) 1534
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 3 cut(s) 824, 1463, 2044
BcuI ACTAGT 2 cut(s) 64, 587
BfaI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1887
BfmI CTRYAG 4 cut(s) 405, 972, 1584, 1620
BfrI CTTAAG 2 cut(s) 865, 1573
BfuAI ACCTGC 3 cut(s) 167, 465, 536
BglI GCCNNNNNGGC 1 cut(s) 1302
BisI GCNGC 5 cut(s) 517, 547, 1193, 1623, 1626
BlsI GCNGC 5 cut(s) 518, 548, 1194, 1624, 1627
BmcAI AGTACT 3 cut(s) 55, 63, 1790
Bme1390I CCNGG 3 cut(s) 469, 999, 2197
Bme18I GGWCC 5 cut(s) 477, 712, 771, 978, 1843
BmeT110I CYCGRG 1 cut(s) 1968
BmgBI CACGTC 1 cut(s) 1825
BmgT120I GGNCC 5 cut(s) 477, 712, 771, 978, 1843
BmiI GGNNCC 5 cut(s) 256, 772, 951, 979, 1782
BmrFI CCNGG 3 cut(s) 469, 999, 2197
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 5 cut(s) 83, 800, 914, 1001, 1954
BmuI ACTGGG 1 cut(s) 4
BpmI CTGGAG 3 cut(s) 284, 1884, 1955
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 1 cut(s) 469
Bsa29I ATCGAT 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 1463
BsaJI CCNNGG 3 cut(s) 723, 1297, 1306
BsaWI WCCGGW 4 cut(s) 952, 1205, 1269, 1783
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 1371, 1401
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 888, 1269
Bse118I RCCGGY 1 cut(s) 1269
Bse1I ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1522, 1867, 1874, 1938
Bse3DI GCAATG 3 cut(s) 538, 823, 2082
BseAI TCCGGA 2 cut(s) 952, 1783
BseBI CCWGG 2 cut(s) 999, 2197
BseCI ATCGAT 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 723, 1297, 1306
BseGI GGATG 6 cut(s) 558, 929, 1010, 1016, 2182, 2215
BseLI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
BseMI GCAATG 3 cut(s) 538, 823, 2082
BseNI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1522, 1867, 1874, 1938
BseXI GCAGC 5 cut(s) 528, 533, 1204, 1612, 1634
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1908
BshNI GGYRCC 1 cut(s) 254
BshTI ACCGGT 1 cut(s) 1269
BshVI ATCGAT 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 1603, 1857
BsiHKCI CYCGRG 1 cut(s) 1968
BsiSI CCGG 6 cut(s) 468, 953, 1206, 1270, 1596, 1784
BslFI GGGAC 6 cut(s) 704, 784, 1191, 1404, 1525, 1723
BslI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
BsmAI GTCTC 3 cut(s) 824, 1463, 2044
BsmBI CGTCTC 1 cut(s) 2044
BsmFI GGGAC 6 cut(s) 704, 784, 1191, 1404, 1525, 1723
BsnI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1908
Bso31I GGTCTC 1 cut(s) 1463
BsoBI CYCGRG 1 cut(s) 1968
Bsp1286I GDGCHC 3 cut(s) 896, 1603, 1857
Bsp13I TCCGGA 2 cut(s) 952, 1783
Bsp1407I TGTACA 2 cut(s) 1113, 1756
Bsp143I GATC 5 cut(s) 649, 949, 1534, 1718, 1780
BspACI CCGC 2 cut(s) 417, 598
BspANI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1908
BspDI ATCGAT 1 cut(s) 794
BspEI TCCGGA 2 cut(s) 952, 1783
BspHI TCATGA 2 cut(s) 190, 2131
BspLI GGNNCC 5 cut(s) 256, 772, 951, 979, 1782
BspMAI CTGCAG 4 cut(s) 409, 976, 1588, 1624
BspMI ACCTGC 3 cut(s) 167, 465, 536
BspPI GGATC 4 cut(s) 944, 957, 1775, 1788
BspQI GCTCTTC 1 cut(s) 853
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 2 cut(s) 865, 1573
BspTNI GGTCTC 1 cut(s) 1463
BsrDI GCAATG 3 cut(s) 538, 823, 2082
BsrFI RCCGGY 1 cut(s) 1269
BsrGI TGTACA 2 cut(s) 1113, 1756
BsrI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1522, 1867, 1874, 1938
BssAI RCCGGY 1 cut(s) 1269
BssECI CCNNGG 3 cut(s) 723, 1297, 1306
BssMI GATC 5 cut(s) 649, 949, 1534, 1718, 1780
BssNAI GTATAC 1 cut(s) 211
BssT1I CCWWGG 2 cut(s) 1297, 1306
Bst1107I GTATAC 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 999, 2197
Bst4CI ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1646, 2008
Bst6I CTCTTC 4 cut(s) 853, 1539, 2042, 2130
BstAFI CTTAAG 2 cut(s) 865, 1573
BstAPI GCANNNNNTGC 2 cut(s) 1592, 1697
BstAUI TGTACA 2 cut(s) 1113, 1756
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 1 cut(s) 897
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 6 cut(s) 558, 929, 1010, 1016, 2182, 2215
BstHHI GCGC 1 cut(s) 1572
BstKTI GATC 5 cut(s) 652, 952, 1537, 1721, 1783
BstMAI GTCTC 3 cut(s) 824, 1463, 2044
BstMBI GATC 5 cut(s) 649, 949, 1534, 1718, 1780
BstMWI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1592, 1697, 2018
BstNI CCWGG 2 cut(s) 999, 2197
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 467, 997, 2195
BstSFI CTRYAG 4 cut(s) 405, 972, 1584, 1620
BstV1I GCAGC 5 cut(s) 528, 533, 1204, 1612, 1634
BstX2I RGATCY 2 cut(s) 949, 1780
BstXI CCANNNNNNTGG 2 cut(s) 709, 1560
BstYI RGATCY 2 cut(s) 949, 1780
BstZ17I GTATAC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 794
BsuRI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1908
BsuTUI ATCGAT 1 cut(s) 794
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1825
BtsCI GGATG 6 cut(s) 558, 929, 1010, 1016, 2182, 2215
BtsI GCAGTG 4 cut(s) 337, 402, 1075, 2217
BtsIMutI CAGTG 8 cut(s) 225, 337, 402, 709, 1075, 1931, 2109, 2217
BveI ACCTGC 3 cut(s) 167, 465, 536
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 2 cut(s) 190, 2131
CfoI GCGC 1 cut(s) 1572
Cfr10I RCCGGY 1 cut(s) 1269
Cfr13I GGNCC 5 cut(s) 477, 712, 771, 978, 1843
ClaI ATCGAT 1 cut(s) 794
CsiI ACCWGGT 1 cut(s) 2195
Csp6I GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1757, 1764, 1789
CspAI ACCGGT 1 cut(s) 1269
CviAII CATG 9 cut(s) 191, 397, 627, 1104, 1532, 1560, 1760, 2062, 2132
CviQI GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1757, 1764, 1789
DdeI CTNAG 1 cut(s) 897
DpnI GATC 5 cut(s) 651, 951, 1536, 1720, 1782
DpnII GATC 5 cut(s) 649, 949, 1534, 1718, 1780
DraI TTTAAA 1 cut(s) 1165
EaeI YGGCCR 3 cut(s) 426, 1294, 1332
Eam1104I CTCTTC 4 cut(s) 853, 1539, 2042, 2130
EarI CTCTTC 4 cut(s) 853, 1539, 2042, 2130
Ecl136II GAGCTC 2 cut(s) 1601, 1855
Eco130I CCWWGG 2 cut(s) 1297, 1306
Eco147I AGGCCT 1 cut(s) 1908
Eco24I GRGCYC 3 cut(s) 896, 1603, 1857
Eco31I GGTCTC 1 cut(s) 1463
Eco32I GATATC 2 cut(s) 934, 1651
Eco47I GGWCC 5 cut(s) 477, 712, 771, 978, 1843
Eco53kI GAGCTC 2 cut(s) 1601, 1855
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1968
EcoICRI GAGCTC 2 cut(s) 1601, 1855
EcoO109I RGGNCCY 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 1496
EcoRII CCWGG 2 cut(s) 997, 2195
EcoRV GATATC 2 cut(s) 934, 1651
EcoT14I CCWWGG 2 cut(s) 1297, 1306
EcoT22I ATGCAT 2 cut(s) 640, 793
EcoT38I GRGCYC 3 cut(s) 896, 1603, 1857
ErhI CCWWGG 2 cut(s) 1297, 1306
Esp3I CGTCTC 1 cut(s) 2044
FaeI CATG 9 cut(s) 194, 400, 630, 1107, 1535, 1563, 1763, 2065, 2135
FalI AAGNNNNNCTT 4 cut(s) 1145, 1177, 1467, 1499
FaqI GGGAC 6 cut(s) 704, 784, 1191, 1404, 1525, 1723
FatI CATG 9 cut(s) 190, 396, 626, 1103, 1531, 1559, 1759, 2061, 2131
FauI CCCGC 1 cut(s) 591
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 1534
FblI GTMKAC 2 cut(s) 210, 1658
Fnu4HI GCNGC 5 cut(s) 517, 547, 1193, 1623, 1626
FokI GGATG 6 cut(s) 545, 936, 1017, 1023, 2189, 2222
FriOI GRGCYC 3 cut(s) 896, 1603, 1857
Fsp4HI GCNGC 5 cut(s) 517, 547, 1193, 1623, 1626
FspBI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1887
GlaI GCGC 1 cut(s) 1571
GluI GCNGC 5 cut(s) 517, 547, 1193, 1623, 1626
GsuI CTGGAG 3 cut(s) 284, 1884, 1955
HaeIII GGCC 5 cut(s) 428, 1296, 1305, 1334, 1908
HapII CCGG 6 cut(s) 468, 953, 1206, 1270, 1596, 1784
HhaI GCGC 1 cut(s) 1572
Hin1II CATG 9 cut(s) 194, 400, 630, 1107, 1535, 1563, 1763, 2065, 2135
Hin6I GCGC 1 cut(s) 1570
HinP1I GCGC 1 cut(s) 1570
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 5 cut(s) 665, 860, 1937, 2092, 2234
HpaII CCGG 6 cut(s) 468, 953, 1206, 1270, 1596, 1784
HphI GGTGA 4 cut(s) 307, 427, 1220, 2101
Hpy166II GTNNAC 6 cut(s) 211, 358, 402, 1318, 1492, 1659
Hpy188I TCNGA 8 cut(s) 79, 305, 481, 962, 1539, 1884, 2116, 2153
Hpy8I GTNNAC 6 cut(s) 211, 358, 402, 1318, 1492, 1659
HpyAV CCTTC 3 cut(s) 281, 328, 589
HpyCH4III ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1646, 2008
HpyCH4IV ACGT 1 cut(s) 1824
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1592, 1697, 2018
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 1 cut(s) 1824
Hsp92II CATG 9 cut(s) 194, 400, 630, 1107, 1535, 1563, 1763, 2065, 2135
HspAI GCGC 1 cut(s) 1570
Kpn2I TCCGGA 2 cut(s) 952, 1783
Ksp22I TGATCA 1 cut(s) 1534
Kzo9I GATC 5 cut(s) 649, 949, 1534, 1718, 1780
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 3 cut(s) 173, 727, 1598
Lsp1109I GCAGC 5 cut(s) 528, 533, 1204, 1612, 1634
LweI GCATC 5 cut(s) 83, 800, 914, 1001, 1954
MabI ACCWGGT 1 cut(s) 2195
MaeI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1887
MaeII ACGT 1 cut(s) 1824
MalI GATC 5 cut(s) 651, 951, 1536, 1720, 1782
MboI GATC 5 cut(s) 649, 949, 1534, 1718, 1780
MfeI CAATTG 1 cut(s) 2229
MflI RGATCY 2 cut(s) 949, 1780
MhlI GDGCHC 3 cut(s) 896, 1603, 1857
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 674, 1946
MmeI TCCRAC 1 cut(s) 2064
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 2 cut(s) 640, 793
MroI TCCGGA 2 cut(s) 952, 1783
MroXI GAANNNNTTC 3 cut(s) 398, 1157, 1548
MscI TGGCCA 1 cut(s) 428
MslI CAYNNNNRTG 3 cut(s) 796, 1437, 1558
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 2 cut(s) 598, 1625
MspCI CTTAAG 2 cut(s) 865, 1573
MspI CCGG 6 cut(s) 468, 953, 1206, 1270, 1596, 1784
MspR9I CCNGG 3 cut(s) 469, 999, 2197
MunI CAATTG 1 cut(s) 2229
MvaI CCWGG 2 cut(s) 999, 2197
MwoI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1592, 1697, 2018
NciI CCSGG 1 cut(s) 469
NdeI CATATG 1 cut(s) 856
NdeII GATC 5 cut(s) 649, 949, 1534, 1718, 1780
NlaIII CATG 9 cut(s) 194, 400, 630, 1107, 1535, 1563, 1763, 2065, 2135
NlaIV GGNNCC 5 cut(s) 256, 772, 951, 979, 1782
NmeAIII GCCGAG 1 cut(s) 1272
NmuCI GTSAC 4 cut(s) 215, 653, 1820, 1890
NsiI ATGCAT 2 cut(s) 640, 793
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 1968
PagI TCATGA 2 cut(s) 190, 2131
PceI AGGCCT 1 cut(s) 1908
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 853
PdmI GAANNNNTTC 3 cut(s) 398, 1157, 1548
PfeI GAWTC 3 cut(s) 860, 2092, 2234
PflFI GACNNNGTC 2 cut(s) 716, 1888
PinAI ACCGGT 1 cut(s) 1269
PkrI GCNGC 5 cut(s) 518, 548, 1194, 1624, 1627
PleI GAGTC 2 cut(s) 673, 1945
PpsI GAGTC 2 cut(s) 673, 1945
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 396
PshBI ATTAAT 1 cut(s) 1401
Psp124BI GAGCTC 2 cut(s) 1603, 1857
Psp5II RGGWCCY 1 cut(s) 771
Psp6I CCWGG 2 cut(s) 997, 2195
PspGI CCWGG 2 cut(s) 997, 2195
PspN4I GGNNCC 5 cut(s) 256, 772, 951, 979, 1782
PspPI GGNCC 5 cut(s) 477, 712, 771, 978, 1843
PspPPI RGGWCCY 1 cut(s) 771
PspXI VCTCGAGB 1 cut(s) 1968
PstI CTGCAG 4 cut(s) 409, 976, 1588, 1624
PsuI RGATCY 2 cut(s) 949, 1780
PsyI GACNNNGTC 2 cut(s) 716, 1888
PvuII CAGCTG 1 cut(s) 1625
RsaI GTAC 9 cut(s) 55, 63, 270, 746, 1115, 1312, 1758, 1765, 1790
RsaNI GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1757, 1764, 1789
RseI CAYNNNNRTG 3 cut(s) 796, 1437, 1558
SacI GAGCTC 2 cut(s) 1603, 1857
SapI GCTCTTC 1 cut(s) 853
SatI GCNGC 5 cut(s) 517, 547, 1193, 1623, 1626
Sau3AI GATC 5 cut(s) 649, 949, 1534, 1718, 1780
Sau96I GGNCC 5 cut(s) 477, 712, 771, 978, 1843
ScaI AGTACT 3 cut(s) 55, 63, 1790
SchI GAGTC 2 cut(s) 674, 1946
ScrFI CCNGG 3 cut(s) 469, 999, 2197
SduI GDGCHC 3 cut(s) 896, 1603, 1857
SexAI ACCWGGT 1 cut(s) 2195
SfaNI GCATC 5 cut(s) 83, 800, 914, 1001, 1954
SfcI CTRYAG 4 cut(s) 405, 972, 1584, 1620
SfiI GGCCNNNNNGGCC 1 cut(s) 1302
Sfr274I CTCGAG 1 cut(s) 1968
SinI GGWCC 5 cut(s) 477, 712, 771, 978, 1843
SlaI CTCGAG 1 cut(s) 1968
SmiMI CAYNNNNRTG 3 cut(s) 796, 1437, 1558
SmlI CTYRAG 4 cut(s) 865, 1148, 1573, 1968
SmoI CTYRAG 4 cut(s) 865, 1148, 1573, 1968
SpeI ACTAGT 2 cut(s) 64, 587
SseBI AGGCCT 1 cut(s) 1908
SsiI CCGC 2 cut(s) 417, 598
SspI AATATT 1 cut(s) 691
SspMI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1887
SstI GAGCTC 2 cut(s) 1603, 1857
StuI AGGCCT 1 cut(s) 1908
StyD4I CCNGG 3 cut(s) 467, 997, 2195
StyI CCWWGG 2 cut(s) 1297, 1306
TaaI ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1646, 2008
TaiI ACGT 1 cut(s) 1827
TaqI TCGA 5 cut(s) 794, 1037, 1851, 1940, 1969
TaqII GACCGA 1 cut(s) 1487
TatI WGTACW 5 cut(s) 53, 61, 1113, 1756, 1788
TfiI GAWTC 3 cut(s) 860, 2092, 2234
TscAI CASTG 8 cut(s) 232, 337, 409, 709, 1075, 1938, 2109, 2224
TseFI GTSAC 4 cut(s) 215, 653, 1820, 1890
TseI GCWGC 5 cut(s) 516, 546, 1192, 1622, 1625
Tsp45I GTSAC 4 cut(s) 215, 653, 1820, 1890
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 8 cut(s) 232, 337, 409, 709, 1075, 1938, 2109, 2224
Tth111I GACNNNGTC 2 cut(s) 716, 1888
Vha464I CTTAAG 2 cut(s) 865, 1573
VpaK11BI GGWCC 5 cut(s) 477, 712, 771, 978, 1843
VspI ATTAAT 1 cut(s) 1401
XapI RAATTY 2 cut(s) 679, 1496
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 706, 2203
XhoI CTCGAG 1 cut(s) 1968
XmiI GTMKAC 2 cut(s) 210, 1658
XmnI GAANNNNTTC 3 cut(s) 398, 1157, 1548
XspI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1887
ZrmI AGTACT 3 cut(s) 55, 63, 1790
Zsp2I ATGCAT 2 cut(s) 640, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.