MD09G1145300.v1.1

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000052
Physical Location & Seq
Forward (+)
11298929 .. 11301415
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1145300.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGGACACCGTCGGCCGGTTTCTACTTCATATTACTTTGTTCTTGTGGTCTATGACTGCGGTAGAAATAGCAGCAACATTAATAGCACCCGTATCAATGCCTGGTTGTCCATCGTATTGTGGTCAAGTCGATCAAATCCCGTATCCTTTTGGAATTGGAACCGGTTGTTACCTTGATGATTGGTTCCAAATAATCTGCGACAACTCCACCAGCCCTCCAAAACCTTTCTTGAACTCTACCGGTCTAGAGGTGCTCGAAATCAATATTGAAGGCACACTAAAGGTGGCGAGCCCTGTTACCTTCTCCAATTGTAGTCACAAGCCATCAGGCCGCCAGACAGCAAACTTAGAGGGAAGCCCTTTTGTGTTCTCCCAGAAGAACAGATTCACTTCAATGAGTTGTGGAGGAATTGGCTTGATGACATCCTTATCCGGATCAACAATTGGAGGCTGCTTGTCCATATGCGACGATACTAGTAATGCTTTAAGAAATAACAGTTGCAGTGGGCTGAATTGCTGCCAGACCACCATTCCTTCATCTCTTAGCACCTTCCGTACTAGTTTCGGTGAAATAACAAATGCGGAGAGAGCAAAATTATGCAAGTATGCTTTCCTAGTAGACCAAGATTGGTTTACATCAGATTCAACTAATATTTCAGACATTGGTCACATGGACAATGTCCCTATAGTTCTGGAATGGAAGTTTTTTAGCAATACGACTTTCAATATAAATGGAACGACAAATTTGACAGATATTGATAAGAACATGGATTGCTCCGGTGATAATTGCTTCTGCTCAAAGGGCTTCCAAGGAAACCCCTATCTTCTTCATGGATGTGAAGATATCGATGAATGTGAGGATCCAGTCCACTTCAACAGATGCATTCCCGGCATTTGTATTAATTATCCCGGGAGGTATCAGTGTGTATTCCCCGATCCACGACAGTCACGAGTTAAGCTGGCCATTATAGTTCCCATCAGTGTTCTTGGACTATTGTTTCTACTAGTTGGTGCATGGTGGCTGCGCAAAGTCATAAAGAAAAGAAAAAATACTAAACGCAAAGAGAAGTTTTTTAAGCAGAATGGTGGTCTATTGTTGAAACAACAATTATCTTCAGGTGAAGTCAATGTTGAGAAGATTAAGTTGTTCAATTCCAAGGAGTTAGAGAAAGCCACAGACAATTTTAGTGCAGACAGAATTCTTGGTCAGGGAGGCCAAGGTACTGTTTATAAAGGAATGCTGGCGGACGGAAGAATAGTTGCTGTCAAGAAATCGAAAATAGTTGCTGGAGGTGAAGTTGGGCAGTTCATTAATGAAATTGTCATTCTTTCACAAATTTCCCACAGGAATGTGGTTAAACTATTGGGGTGTTGTTTAGAGACCGAAGTTCCCCTTTTGGTTTATGAATTCATACTCAATGGAACACTTTCTCAGTATATTCACCACCAAAATGAAGAGTTCCCCCTCACATGGGAAATGAGGTTGCGAGTTTCCATCGAAGTTGCAGGAGCTCTTTCCTACTTACATTCAGCAGCTTCCTTTCCCATTTACCACCGCGACATCAAGTCTTCTAACATACTCTTGGATGAAAAATACAGAGCCAAAGTAGCAGACTTTGGGACTTCAAGATCAGTTGCCATTGATCAAACTCACCTTACAACACGAGTACATGGAACATTTGGTTATCTGGACCCAGAGTACTTCCAATCTAGCAAATTTACAGAGAAGAGTGATGTTTATAGCTTTGGAGTGGTCCTTGCGGAGCTCTTGACAGGAGAAAAACCAGTTTCAGTTTTGAGTTCGCAAGAAAGCAGAAGCCTAGCAACTTATTTTCTTCTTTCTATGGAGGAGAATCGTCTATTTGATATTCTTCATGCTCGAGTAATGAAGGAGGCTGGGAAAGACAAGATTATGGCAGTTGCTAACCTTGCACAGAGGTGCTTGAATTTGAATGGGAAGAAACGACCCACCATGAAAGAAGTAGCAGCGGAGTTGGAGGCAATTCAATTGTCAGTTAAATTTTCTAATGTGCAACAAAAGTTTGCAGAAGTTCATTATGTTCGAAGTGAAATAACCGAGCCCTGGGATGTTGTTTCTATATCAACAGCTTCTTGTATGGATAGTGGCACAGGCTCTTCATTGGATGTACAACCACTATTGTCCTTCAAGTCATTCTGA

Protein Analysis

726

Amino Acids

80.31

Weight (kDa)

6.55

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 96 5.9e-10 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 397 - 662 3.1e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 398 - 665 4.7e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc16I TGCGCA 1 cut(s) 1025
AccI GTMKAC 1 cut(s) 618
AccII CGCG 1 cut(s) 1557
AccIII TCCGGA 1 cut(s) 431
AciI CCGC 7 cut(s) 59, 331, 581, 1244, 1555, 1760, 1988
AclWI GGATC 4 cut(s) 442, 854, 867, 929
AcoI YGGCCR 2 cut(s) 13, 960
AcsI RAATTY 7 cut(s) 742, 1197, 1335, 1406, 1715, 1945, 2018
AcuI CTGAAG 1 cut(s) 1098
AfaI GTAC 5 cut(s) 556, 1222, 1668, 1700, 2148
AfiI CCNNNNNNNGG 4 cut(s) 15, 1470, 1471, 2082
AgeI ACCGGT 2 cut(s) 161, 239
AhdI GACNNNNNGTC 1 cut(s) 1564
AhlI ACTAGT 3 cut(s) 473, 557, 1003
AjnI CCWGG 2 cut(s) 100, 2081
AleI CACNNNNGTG 1 cut(s) 1936
AluBI AGCT 6 cut(s) 958, 1511, 1535, 1743, 1765, 2108
AluI AGCT 6 cut(s) 958, 1511, 1535, 1743, 1765, 2108
Alw21I GWGCWC 3 cut(s) 255, 1513, 1767
Alw26I GTCTC 1 cut(s) 1373
AlwI GGATC 4 cut(s) 442, 854, 867, 929
Ama87I CYCGRG 2 cut(s) 908, 1878
Aor13HI TCCGGA 1 cut(s) 431
AoxI GGCC 4 cut(s) 13, 328, 960, 1213
ApeKI GCWGC 6 cut(s) 71, 450, 516, 1021, 1532, 1985
ApoI RAATTY 7 cut(s) 742, 1197, 1335, 1406, 1715, 1945, 2018
AseI ATTAAT 3 cut(s) 80, 900, 1311
AsiGI ACCGGT 2 cut(s) 161, 239
Asp700I GAANNNNTTC 2 cut(s) 383, 1426
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 2 cut(s) 1690, 1753
AsuC2I CCSGG 3 cut(s) 888, 909, 910
AsuHPI GGTGA 6 cut(s) 578, 791, 1130, 1304, 1433, 1643
AsuII TTCGAA 1 cut(s) 2062
AvaI CYCGRG 2 cut(s) 908, 1878
AvaII GGWCC 2 cut(s) 1690, 1753
BalI TGGCCA 1 cut(s) 962
BamHI GGATCC 1 cut(s) 859
BanII GRGCYC 4 cut(s) 293, 1513, 1767, 2082
BauI CACGAG 2 cut(s) 948, 1662
BbsI GAAGAC 1 cut(s) 1560
Bbv12I GWGCWC 3 cut(s) 255, 1513, 1767
BbvI GCAGC 6 cut(s) 83, 437, 503, 1008, 1544, 1997
BccI CCATC 4 cut(s) 118, 331, 983, 1502
BciT130I CCWGG 2 cut(s) 102, 2083
BciVI GTATCC 1 cut(s) 153
BclI TGATCA 1 cut(s) 1642
BcnI CCSGG 3 cut(s) 888, 909, 910
BcoDI GTCTC 1 cut(s) 1373
BcuI ACTAGT 3 cut(s) 473, 557, 1003
BfaI CTAG 7 cut(s) 245, 474, 558, 614, 1004, 1710, 1820
BfmI CTRYAG 1 cut(s) 684
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 7 cut(s) 72, 331, 451, 517, 1022, 1533, 1986
BlsI GCNGC 7 cut(s) 73, 332, 452, 518, 1023, 1534, 1987
BmcAI AGTACT 1 cut(s) 1700
Bme1390I CCNGG 5 cut(s) 102, 888, 909, 910, 2083
Bme18I GGWCC 2 cut(s) 1690, 1753
BmeRI GACNNNNNGTC 1 cut(s) 1564
BmeT110I CYCGRG 2 cut(s) 908, 1878
BmgT120I GGNCC 2 cut(s) 1690, 1753
BmiI GGNNCC 4 cut(s) 160, 185, 861, 1692
BmrFI CCNGG 5 cut(s) 102, 888, 909, 910, 2083
BmsI GCATC 1 cut(s) 869
BoxI GACNNNNGTC 1 cut(s) 663
BpiI GAAGAC 1 cut(s) 1560
BpmI CTGGAG 1 cut(s) 1308
Bpu14I TTCGAA 1 cut(s) 2062
BpuMI CCSGG 3 cut(s) 888, 909, 910
Bsa29I ATCGAT 1 cut(s) 846
BsaI GGTCTC 1 cut(s) 1373
BsaJI CCNNGG 6 cut(s) 808, 908, 1155, 1216, 2081, 2082
BsaWI WCCGGW 4 cut(s) 161, 239, 431, 776
BsaXI ACNNNNNCTCC 4 cut(s) 199, 229, 1281, 1311
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 1470, 1471, 2082
Bse118I RCCGGY 3 cut(s) 15, 161, 239
Bse1I ACTGG 2 cut(s) 863, 1784
BseAI TCCGGA 1 cut(s) 431
BseBI CCWGG 2 cut(s) 102, 2083
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 6 cut(s) 808, 908, 1155, 1216, 2081, 2082
BseGI GGATG 5 cut(s) 422, 839, 1591, 2092, 2149
BseLI CCNNNNNNNGG 4 cut(s) 15, 1470, 1471, 2082
BseMII CTCAG 1 cut(s) 1445
BseNI ACTGG 2 cut(s) 863, 1784
BseRI GAGGAG 1 cut(s) 1862
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 6 cut(s) 83, 437, 503, 1008, 1544, 1997
BseYI CCCAGC 1 cut(s) 1895
BsgI GTGCAG 1 cut(s) 1209
Bsh1236I CGCG 1 cut(s) 1557
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 4 cut(s) 15, 330, 962, 1215
BshTI ACCGGT 2 cut(s) 161, 239
BshVI ATCGAT 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 3 cut(s) 255, 1513, 1767
BsiHKCI CYCGRG 2 cut(s) 908, 1878
BsiSI CCGG 7 cut(s) 16, 162, 240, 432, 777, 888, 909
BslFI GGGAC 2 cut(s) 665, 1633
BslI CCNNNNNNNGG 4 cut(s) 15, 1470, 1471, 2082
BsmAI GTCTC 1 cut(s) 1373
BsmFI GGGAC 2 cut(s) 665, 1633
BsmI GAATGC 2 cut(s) 882, 1242
BsnI GGCC 4 cut(s) 15, 330, 962, 1215
Bso31I GGTCTC 1 cut(s) 1373
BsoBI CYCGRG 2 cut(s) 908, 1878
Bsp119I TTCGAA 1 cut(s) 2062
Bsp1286I GDGCHC 5 cut(s) 255, 293, 1513, 1767, 2082
Bsp13I TCCGGA 1 cut(s) 431
Bsp1407I TGTACA 1 cut(s) 2146
Bsp143I GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
BspACI CCGC 7 cut(s) 59, 331, 581, 1244, 1555, 1760, 1988
BspANI GGCC 4 cut(s) 15, 330, 962, 1215
BspCNI CTCAG 1 cut(s) 1444
BspDI ATCGAT 1 cut(s) 846
BspEI TCCGGA 1 cut(s) 431
BspFNI CGCG 1 cut(s) 1557
BspLI GGNNCC 4 cut(s) 160, 185, 861, 1692
BspPI GGATC 4 cut(s) 442, 854, 867, 929
BspQI GCTCTTC 1 cut(s) 2140
BspT104I TTCGAA 1 cut(s) 2062
BspTNI GGTCTC 1 cut(s) 1373
BsrFI RCCGGY 3 cut(s) 15, 161, 239
BsrGI TGTACA 1 cut(s) 2146
BsrI ACTGG 2 cut(s) 863, 1784
BssAI RCCGGY 3 cut(s) 15, 161, 239
BssECI CCNNGG 6 cut(s) 808, 908, 1155, 1216, 2081, 2082
BssMI GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
BssSI CACGAG 2 cut(s) 948, 1662
BssT1I CCWWGG 3 cut(s) 808, 1155, 1216
Bst2BI CACGAG 2 cut(s) 948, 1662
Bst2UI CCWGG 2 cut(s) 102, 2083
Bst4CI ACNGT 4 cut(s) 10, 497, 945, 1225
Bst6I CTCTTC 3 cut(s) 1449, 1721, 2140
BstAUI TGTACA 1 cut(s) 2146
BstBI TTCGAA 1 cut(s) 2062
BstC8I GCNNGC 3 cut(s) 289, 960, 1242
BstDEI CTNAG 3 cut(s) 346, 542, 1431
BstF5I GGATG 5 cut(s) 422, 839, 1591, 2092, 2149
BstFNI CGCG 1 cut(s) 1557
BstHHI GCGC 1 cut(s) 1026
BstKTI GATC 6 cut(s) 133, 437, 862, 937, 1631, 1645
BstMAI GTCTC 1 cut(s) 1373
BstMBI GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 5 cut(s) 587, 801, 888, 1607, 1928
BstNI CCWGG 2 cut(s) 102, 2083
BstPAI GACNNNNGTC 1 cut(s) 663
BstSCI CCNGG 5 cut(s) 100, 886, 907, 908, 2081
BstSFI CTRYAG 1 cut(s) 684
BstUI CGCG 1 cut(s) 1557
BstV1I GCAGC 6 cut(s) 83, 437, 503, 1008, 1544, 1997
BstV2I GAAGAC 1 cut(s) 1560
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
BstZI CGGCCG 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 846
BsuI GTATCC 1 cut(s) 153
BsuRI GGCC 4 cut(s) 15, 330, 962, 1215
BsuTUI ATCGAT 1 cut(s) 846
BtsCI GGATG 5 cut(s) 422, 839, 1591, 2092, 2149
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 3 cut(s) 508, 926, 985
Cac8I GCNNGC 3 cut(s) 289, 960, 1242
CfoI GCGC 1 cut(s) 1026
Cfr10I RCCGGY 3 cut(s) 15, 161, 239
Cfr13I GGNCC 2 cut(s) 1690, 1753
Cfr9I CCCGGG 1 cut(s) 908
ClaI ATCGAT 1 cut(s) 846
Csp6I GTAC 5 cut(s) 555, 1221, 1667, 1699, 2147
CspAI ACCGGT 2 cut(s) 161, 239
CviAII CATG 8 cut(s) 670, 766, 830, 1014, 1470, 1670, 1874, 1972
CviQI GTAC 5 cut(s) 555, 1221, 1667, 1699, 2147
DdeI CTNAG 3 cut(s) 346, 542, 1431
DpnI GATC 6 cut(s) 132, 436, 861, 936, 1630, 1644
DpnII GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
DriI GACNNNNNGTC 1 cut(s) 1564
EaeI YGGCCR 2 cut(s) 13, 960
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 3 cut(s) 1449, 1721, 2140
Eam1105I GACNNNNNGTC 1 cut(s) 1564
EarI CTCTTC 3 cut(s) 1449, 1721, 2140
EciI GGCGGA 1 cut(s) 1259
Ecl136II GAGCTC 2 cut(s) 1511, 1765
EclXI CGGCCG 1 cut(s) 13
Eco130I CCWWGG 3 cut(s) 808, 1155, 1216
Eco24I GRGCYC 4 cut(s) 293, 1513, 1767, 2082
Eco31I GGTCTC 1 cut(s) 1373
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 2 cut(s) 1690, 1753
Eco52I CGGCCG 1 cut(s) 13
Eco53kI GAGCTC 2 cut(s) 1511, 1765
Eco57I CTGAAG 1 cut(s) 1098
Eco88I CYCGRG 2 cut(s) 908, 1878
EcoICRI GAGCTC 2 cut(s) 1511, 1765
EcoRI GAATTC 2 cut(s) 1197, 1406
EcoRII CCWGG 2 cut(s) 100, 2081
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 3 cut(s) 808, 1155, 1216
EcoT22I ATGCAT 1 cut(s) 884
EcoT38I GRGCYC 4 cut(s) 293, 1513, 1767, 2082
ErhI CCWWGG 3 cut(s) 808, 1155, 1216
FaeI CATG 8 cut(s) 673, 769, 833, 1017, 1473, 1673, 1877, 1975
FalI AAGNNNNNCTT 2 cut(s) 1377, 1409
FaqI GGGAC 2 cut(s) 665, 1633
FatI CATG 8 cut(s) 669, 765, 829, 1013, 1469, 1669, 1873, 1971
FauNDI CATATG 1 cut(s) 461
FbaI TGATCA 1 cut(s) 1642
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 7 cut(s) 72, 331, 451, 517, 1022, 1533, 1986
FokI GGATG 5 cut(s) 409, 846, 1598, 2099, 2156
FriOI GRGCYC 4 cut(s) 293, 1513, 1767, 2082
Fsp4HI GCNGC 7 cut(s) 72, 331, 451, 517, 1022, 1533, 1986
FspBI CTAG 7 cut(s) 245, 474, 558, 614, 1004, 1710, 1820
FspI TGCGCA 1 cut(s) 1025
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 7 cut(s) 72, 331, 451, 517, 1022, 1533, 1986
GsaI CCCAGC 1 cut(s) 1899
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 4 cut(s) 15, 330, 962, 1215
HapII CCGG 7 cut(s) 16, 162, 240, 432, 777, 888, 909
HhaI GCGC 1 cut(s) 1026
Hin1II CATG 8 cut(s) 673, 769, 833, 1017, 1473, 1673, 1877, 1975
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HinfI GANTC 3 cut(s) 384, 641, 1852
HpaII CCGG 7 cut(s) 16, 162, 240, 432, 777, 888, 909
HphI GGTGA 6 cut(s) 578, 791, 1130, 1304, 1433, 1643
Hpy166II GTNNAC 3 cut(s) 619, 633, 868
Hpy188I TCNGA 3 cut(s) 640, 658, 2177
Hpy188III TCNNGA 9 cut(s) 229, 245, 432, 692, 948, 1267, 1626, 1688, 1768
Hpy8I GTNNAC 3 cut(s) 619, 633, 868
Hpy99I CGWCG 2 cut(s) 14, 470
HpyAV CCTTC 6 cut(s) 263, 310, 543, 559, 1882, 2173
HpyCH4III ACNGT 4 cut(s) 10, 497, 945, 1225
HpyCH4V TGCA 9 cut(s) 501, 600, 882, 1013, 1190, 1505, 1931, 2032, 2045
HpyF10VI GCNNNNNNNGC 5 cut(s) 587, 801, 888, 1607, 1928
HpyF3I CTNAG 3 cut(s) 346, 542, 1431
Hsp92II CATG 8 cut(s) 673, 769, 833, 1017, 1473, 1673, 1877, 1975
HspAI GCGC 1 cut(s) 1024
Kpn2I TCCGGA 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 1642
Kzo9I GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
LguI GCTCTTC 1 cut(s) 2140
LmnI GCTCC 3 cut(s) 779, 1508, 1762
Lsp1109I GCAGC 6 cut(s) 83, 437, 503, 1008, 1544, 1997
LweI GCATC 1 cut(s) 869
MaeI CTAG 7 cut(s) 245, 474, 558, 614, 1004, 1710, 1820
MaeIII GTNAC 5 cut(s) 167, 295, 314, 665, 945
MalI GATC 6 cut(s) 132, 436, 861, 936, 1630, 1644
MboI GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
MfeI CAATTG 3 cut(s) 307, 441, 2006
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 5 cut(s) 255, 293, 1513, 1767, 2082
MlsI TGGCCA 1 cut(s) 962
MluNI TGGCCA 1 cut(s) 962
MmeI TCCRAC 1 cut(s) 1974
Mox20I TGGCCA 1 cut(s) 962
Mph1103I ATGCAT 1 cut(s) 884
MroI TCCGGA 1 cut(s) 431
MroXI GAANNNNTTC 2 cut(s) 383, 1426
MscI TGGCCA 1 cut(s) 962
MseI TTAA 9 cut(s) 80, 485, 900, 954, 1074, 1140, 1311, 1356, 2016
MslI CAYNNNNRTG 5 cut(s) 392, 834, 1347, 1449, 1936
Msp20I TGGCCA 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 1988
MspI CCGG 7 cut(s) 16, 162, 240, 432, 777, 888, 909
MspR9I CCNGG 5 cut(s) 102, 888, 909, 910, 2083
MunI CAATTG 3 cut(s) 307, 441, 2006
Mva1269I GAATGC 2 cut(s) 882, 1242
MvaI CCWGG 2 cut(s) 102, 2083
MvnI CGCG 1 cut(s) 1557
MwoI GCNNNNNNNGC 5 cut(s) 587, 801, 888, 1607, 1928
NciI CCSGG 3 cut(s) 888, 909, 910
NdeI CATATG 1 cut(s) 461
NdeII GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
NlaIII CATG 8 cut(s) 673, 769, 833, 1017, 1473, 1673, 1877, 1975
NlaIV GGNNCC 4 cut(s) 160, 185, 861, 1692
NmuCI GTSAC 3 cut(s) 314, 665, 945
NsbI TGCGCA 1 cut(s) 1025
NsiI ATGCAT 1 cut(s) 884
NspV TTCGAA 1 cut(s) 2062
OliI CACNNNNGTG 1 cut(s) 1936
PaeR7I CTCGAG 1 cut(s) 1878
PasI CCCWGGG 1 cut(s) 2082
PciSI GCTCTTC 1 cut(s) 2140
PcsI WCGNNNNNNNCGW 1 cut(s) 946
PctI GAATGC 2 cut(s) 882, 1242
PdmI GAANNNNTTC 2 cut(s) 383, 1426
PfeI GAWTC 3 cut(s) 384, 641, 1852
PflFI GACNNNGTC 2 cut(s) 8, 677
PinAI ACCGGT 2 cut(s) 161, 239
PkrI GCNGC 7 cut(s) 73, 332, 452, 518, 1023, 1534, 1987
PshAI GACNNNNGTC 1 cut(s) 663
PshBI ATTAAT 3 cut(s) 80, 900, 1311
PsiI TTATAA 1 cut(s) 1230
Psp124BI GAGCTC 2 cut(s) 1513, 1767
Psp6I CCWGG 2 cut(s) 100, 2081
PspFI CCCAGC 1 cut(s) 1895
PspGI CCWGG 2 cut(s) 100, 2081
PspN4I GGNNCC 4 cut(s) 160, 185, 861, 1692
PspPI GGNCC 2 cut(s) 1690, 1753
PspXI VCTCGAGB 1 cut(s) 1878
PsuI RGATCY 1 cut(s) 859
PsyI GACNNNGTC 2 cut(s) 8, 677
RsaI GTAC 5 cut(s) 556, 1222, 1668, 1700, 2148
RsaNI GTAC 5 cut(s) 555, 1221, 1667, 1699, 2147
RseI CAYNNNNRTG 5 cut(s) 392, 834, 1347, 1449, 1936
SacI GAGCTC 2 cut(s) 1513, 1767
SapI GCTCTTC 1 cut(s) 2140
SaqAI TTAA 9 cut(s) 80, 485, 900, 954, 1074, 1140, 1311, 1356, 2016
SatI GCNGC 7 cut(s) 72, 331, 451, 517, 1022, 1533, 1986
Sau3AI GATC 6 cut(s) 130, 434, 859, 934, 1628, 1642
Sau96I GGNCC 2 cut(s) 1690, 1753
ScaI AGTACT 1 cut(s) 1700
ScrFI CCNGG 5 cut(s) 102, 888, 909, 910, 2083
SduI GDGCHC 5 cut(s) 255, 293, 1513, 1767, 2082
SfaNI GCATC 1 cut(s) 869
SfcI CTRYAG 1 cut(s) 684
Sfr274I CTCGAG 1 cut(s) 1878
SfuI TTCGAA 1 cut(s) 2062
SinI GGWCC 2 cut(s) 1690, 1753
SlaI CTCGAG 1 cut(s) 1878
SmaI CCCGGG 1 cut(s) 910
SmiMI CAYNNNNRTG 5 cut(s) 392, 834, 1347, 1449, 1936
SmlI CTYRAG 1 cut(s) 1878
SmoI CTYRAG 1 cut(s) 1878
SpeI ACTAGT 3 cut(s) 473, 557, 1003
SsiI CCGC 7 cut(s) 59, 331, 581, 1244, 1555, 1760, 1988
SspI AATATT 2 cut(s) 265, 652
SspMI CTAG 7 cut(s) 245, 474, 558, 614, 1004, 1710, 1820
SstI GAGCTC 2 cut(s) 1513, 1767
StyD4I CCNGG 5 cut(s) 100, 886, 907, 908, 2081
StyI CCWWGG 3 cut(s) 808, 1155, 1216
TaaI ACNGT 4 cut(s) 10, 497, 945, 1225
TaqI TCGA 7 cut(s) 129, 255, 846, 1274, 1497, 1879, 2062
TaqII GACCGA 1 cut(s) 1397
TatI WGTACW 3 cut(s) 1666, 1698, 2146
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 3 cut(s) 384, 641, 1852
Tru1I TTAA 9 cut(s) 80, 485, 900, 954, 1074, 1140, 1311, 1356, 2016
Tru9I TTAA 9 cut(s) 80, 485, 900, 954, 1074, 1140, 1311, 1356, 2016
TscAI CASTG 3 cut(s) 508, 926, 985
TseFI GTSAC 3 cut(s) 314, 665, 945
TseI GCWGC 6 cut(s) 71, 450, 516, 1021, 1532, 1985
Tsp45I GTSAC 3 cut(s) 314, 665, 945
TspGWI ACGGA 2 cut(s) 542, 1263
TspMI CCCGGG 1 cut(s) 908
TspRI CASTG 3 cut(s) 508, 926, 985
Tth111I GACNNNGTC 2 cut(s) 8, 677
VpaK11BI GGWCC 2 cut(s) 1690, 1753
VspI ATTAAT 3 cut(s) 80, 900, 1311
XapI RAATTY 7 cut(s) 742, 1197, 1335, 1406, 1715, 1945, 2018
XbaI TCTAGA 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 1878
XmaI CCCGGG 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 2 cut(s) 383, 1426
XspI CTAG 7 cut(s) 245, 474, 558, 614, 1004, 1710, 1820
ZrmI AGTACT 1 cut(s) 1700
Zsp2I ATGCAT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.