Rh2CG357200

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000052
Physical Location & Seq
Reverse (-)
48367813 .. 48381510
13698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG357200.1

Sequence Viewer

Length: 2289 bp
ATGCAATGGCTAGTTCAGGACGTGATTTTCATTCTATTGTTATATAAATGGCGCATAAGTACAGCATCATCATCACCACTGAAGATAGCCAAGGAGAACTGTACACCGCAGTGCGGAGCTGTTAGCATCCCATACCCGTTTGGGATTGGACGTCCTAATAACCATTGTTATTTTGACGAGTGGTTCGAAATAGAATGCAAACCTTCTTTTCCAGTAGCGAAGCCGTTCTTGAGGCGTCTACAACTGGAGGTGCTGAATATTTCTGTTTATGGTGGAAACACAACTGTTCAGGTTCCAAGCCCTGTTACTTGCTTGAGTTGCAAGGGGAAGCAATCTCCGCCAGCGCCAAATCTGACCCGAAGCCCTTTTATGTACTCTTCTGAAAACAGTTTCGTTGCAGTCAGTTGTGATTCATTTGCCTCGCTACGCACAGATCAAACGCGCACTCTTACTGGGTGCTCGTCAACTTGTCTTGATCAAGATATGTTTTCCGCTTCTGAAATGTGCGAGTATGGTTATAATTGTTGCCGGACTGACCTTTCCCGATCTATCACTACTTTCAGCATCACACAAGAACGAGATGAAACAAGAAGAAATAAGACGGATTACGAGGACTATGCTTTCCTGGTTGACCAACAGTGGTTTGATGAACATAAATCAGACTTCAGAGCTATCAAGGATAGGGACGTCGTTCCTGTGAAATTAGACTGGATCTTAAGCTTAGAAAAAAATAAGTCCTTGATCAAATCATTTGAAGCAGTCAACAAAGTAGACGACCGGAGGAACCCAAATGACTCAACACCCATATGCTATCCATATAGTTTTCTGAGGAATTACACAAAGTCTGATTTGTATTTTTGTGAGTGTCCGTCAGGCTATGAAGGAAATCCCTACCTGCTCCTACCTTGTCGAGATATTGATGAATGCAAGGATAGTAATCCGTGCCAGGGTGATGATCTTAATACAACTAATACATGGAACATTAGTAATGCCAAATGTCAGAACACTGTCGGGGGTCATATATGCTACTCAAATAGAACAGGGGAAACCTGCGAATTGTTTGCCTCTGACAGAGTTGCGAAGTGCTATCATAAAAGTAGGCCTCGCTCTCAGCTCAAGCCTATTCTCTCAGGTCTCGGTGCTGGTATTGGACTGTTGTCATTGCTTAGTGGTGCATGGTGGGTATACAAGTTTGTAAAGAAATGGAAGAAGATTAAACGTAAGGAGATGTTTTACAAACGAAATGGTGGTCTATTGCTAGAACAACAACTATCGTCAGGTGATATTAACGTTGAGAGAATCAAATTGTTCAAATGCAAGGAGTTACAGAGGTCTACAAACAATTTCAATGCTGATAGAATTATTGGTCAAGGGGGGCAAGGTACTGTTTATAAAGGCATGTTGATGGATGGAAGAATTGTGGCTGTTAAAAAGTCAAAAATTGTAGACAAGGCAAAACTTTCAGAATTCATCAATGAGGTTGTCATTCTTTCTCAAATCAACCACAGGAATATCGTTCAACTATTGGGTTGTTGTCTAGAGACAGAAGTTCCTCTTTTGGTATATGAGTTTATACCTAATGGAAACTTATCTCAGTACATCCATGAGCAGACCGAAGAATTTCCACTTACATGGGAGATTCGTTTACGAATTGCTACAGAAATTGCAGGAGCTCTTTCCTACTTACATGCTTCAGCTTCGTTCCCCATTTATCACAGAGACATCAAGTCCACAAACATACTCTTGGATGATAAATACAGAGCAAAAATTGCTGACTTTGGAACTTCAAGATTTGTTGCCATTGACCAAACTCACTTGACCACAAACGTACATGGCACATTTGGTTATTTGGATCCTGAATACTACCAATCAAGTCAATTTACTGAGAAAAGTGACGTCTACAGCTTTGGAGTGGTGCTTGTTGAGCTCTTGACAGGAAAAAAACCAGTAACAAGGTCAGCCGAAGGCGAAACATACAAGAGTCTTGCCACATATTTTATTGTTTCGTTGCAGGAAGATTCTCTATTTGACATTCTTGATGCTCGAGTTGTGAATGAAGGCTCTAAAGATGGTATTATGGTAGTCGCTAATCTTGCAAAACGATGTTTAAATTTGAATGGAAGAAAGCGCCCTACGATGAGAGAGATCACAGCAGAGTTGGAGGCCATCCAATTATCGGAGAAACTTGCCGACGCTCGTCAACAAAGTTGTGAAAGGATTGAATTTGGCCAAGATTATCGAATCGAGCAGTGGGATGACGTTGTTTCTAGCTCCACAATGTCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

762

Amino Acids

86.62

Weight (kDa)

7.06

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 90 9.8e-10 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 450 - 717 1.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 451 - 720 1.1e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 519, 1392
AatII GACGTC 3 cut(s) 154, 690, 1899
Acc36I ACCTGC 2 cut(s) 903, 1058
AccI GTMKAC 6 cut(s) 238, 771, 1185, 1334, 1446, 1899
AccII CGCG 1 cut(s) 442
AciI CCGC 4 cut(s) 107, 114, 338, 492
AclI AACGTT 1 cut(s) 1290
AclWI GGATC 3 cut(s) 719, 1847, 1860
AcoI YGGCCR 1 cut(s) 2227
AcsI RAATTY 4 cut(s) 1466, 1619, 2110, 2222
AcuI CTGAAG 3 cut(s) 101, 649, 1677
AcyI GRCGYC 4 cut(s) 151, 235, 687, 1896
AfaI GTAC 6 cut(s) 61, 103, 374, 1384, 1598, 1830
AfiI CCNNNNNNNGG 3 cut(s) 113, 947, 2176
AflII CTTAAG 1 cut(s) 715
AgsI TTSAA 7 cut(s) 755, 1312, 1348, 1520, 1788, 2116, 2222
AhdI GACNNNNNGTC 1 cut(s) 1726
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 624, 945
AleI CACNNNNGTG 1 cut(s) 109
AluBI AGCT 9 cut(s) 119, 671, 720, 1114, 1673, 1697, 1905, 1927, 2271
AluI AGCT 9 cut(s) 119, 671, 720, 1114, 1673, 1697, 1905, 1927, 2271
Alw21I GWGCWC 3 cut(s) 461, 1675, 1929
Alw26I GTCTC 3 cut(s) 1139, 1535, 1713
AlwI GGATC 3 cut(s) 719, 1847, 1860
Ama87I CYCGRG 1 cut(s) 2043
AoxI GGCC 3 cut(s) 1100, 2163, 2227
ApoI RAATTY 4 cut(s) 1466, 1619, 2110, 2222
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 1234, 1266
Asp700I GAANNNNTTC 2 cut(s) 224, 1620
AspLEI GCGC 4 cut(s) 54, 346, 444, 2130
AsuHPI GGTGA 3 cut(s) 66, 962, 1292
AsuII TTCGAA 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 2043
BalI TGGCCA 1 cut(s) 2229
BamHI GGATCC 1 cut(s) 1852
BanII GRGCYC 2 cut(s) 1675, 1929
Bbv12I GWGCWC 3 cut(s) 461, 1675, 1929
BccI CCATC 4 cut(s) 1399, 1403, 2063, 2174
BceAI ACGGC 1 cut(s) 208
BcgI CGANNNNNNTGC 4 cut(s) 599, 633, 1043, 1077
BciT130I CCWGG 2 cut(s) 626, 947
BclI TGATCA 2 cut(s) 475, 741
BcoDI GTCTC 3 cut(s) 1139, 1535, 1713
BfaI CTAG 4 cut(s) 11, 1259, 1538, 2268
BfmI CTRYAG 2 cut(s) 1656, 1900
BfoI RGCGCY 2 cut(s) 347, 2131
BfrI CTTAAG 1 cut(s) 715
BfuAI ACCTGC 2 cut(s) 903, 1058
Bme1390I CCNGG 2 cut(s) 626, 947
BmeRI GACNNNNNGTC 1 cut(s) 1726
BmeT110I CYCGRG 1 cut(s) 2043
BmgBI CACGTC 1 cut(s) 22
BmiI GGNNCC 3 cut(s) 294, 785, 1854
BmrFI CCNGG 2 cut(s) 626, 947
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 4 cut(s) 74, 135, 573, 2029
BmuI ACTGGG 1 cut(s) 462
BoxI GACNNNNGTC 2 cut(s) 1156, 2196
BpmI CTGGAG 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 3 cut(s) 250, 334, 1100
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 4 cut(s) 151, 235, 687, 1896
BsaI GGTCTC 1 cut(s) 1139
BsaJI CCNNGG 2 cut(s) 90, 946
BsaWI WCCGGW 1 cut(s) 777
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 947, 2176
Bse1I ACTGG 5 cut(s) 212, 249, 457, 713, 1946
Bse3DI GCAATG 2 cut(s) 11, 1160
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 2 cut(s) 626, 947
BseDI CCNNGG 2 cut(s) 90, 946
BseGI GGATG 6 cut(s) 126, 1414, 1599, 1753, 2166, 2260
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 3 cut(s) 113, 947, 2176
BseMI GCAATG 2 cut(s) 11, 1160
BseMII CTCAG 5 cut(s) 818, 1124, 1143, 1607, 1875
BseNI ACTGG 5 cut(s) 212, 249, 457, 713, 1946
Bsh1236I CGCG 1 cut(s) 442
Bsh1285I CGRYCG 1 cut(s) 778
BshFI GGCC 3 cut(s) 1102, 2165, 2229
BsiEI CGRYCG 1 cut(s) 778
BsiHKAI GWGCWC 3 cut(s) 461, 1675, 1929
BsiHKCI CYCGRG 1 cut(s) 2043
BsiSI CCGG 2 cut(s) 529, 778
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 3 cut(s) 113, 947, 2176
BsmAI GTCTC 3 cut(s) 1139, 1535, 1713
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 2 cut(s) 200, 929
BsnI GGCC 3 cut(s) 1102, 2165, 2229
Bso31I GGTCTC 1 cut(s) 1139
BsoBI CYCGRG 1 cut(s) 2043
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 3 cut(s) 461, 1675, 1929
Bsp1407I TGTACA 1 cut(s) 101
Bsp143I GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
BspACI CCGC 4 cut(s) 107, 114, 338, 492
BspANI GGCC 3 cut(s) 1102, 2165, 2229
BspCNI CTCAG 5 cut(s) 819, 1123, 1142, 1606, 1876
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 3 cut(s) 294, 785, 1854
BspMI ACCTGC 2 cut(s) 903, 1058
BspPI GGATC 3 cut(s) 719, 1847, 1860
BspT104I TTCGAA 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 1139
BsrDI GCAATG 2 cut(s) 11, 1160
BsrGI TGTACA 1 cut(s) 101
BsrI ACTGG 5 cut(s) 212, 249, 457, 713, 1946
BssECI CCNNGG 2 cut(s) 90, 946
BssMI GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
BssNAI GTATAC 1 cut(s) 1186
BssNI GRCGYC 4 cut(s) 151, 235, 687, 1896
BssT1I CCWWGG 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 1186
Bst2UI CCWGG 2 cut(s) 626, 947
Bst4CI ACNGT 7 cut(s) 101, 286, 389, 639, 1009, 1155, 1387
Bst6I CTCTTC 1 cut(s) 382
BstACI GRCGYC 4 cut(s) 151, 235, 687, 1896
BstAFI CTTAAG 1 cut(s) 715
BstAPI GCANNNNNTGC 1 cut(s) 1769
BstAUI TGTACA 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 8 cut(s) 721, 827, 1110, 1129, 1166, 1593, 1884, 2286
BstF5I GGATG 6 cut(s) 126, 1414, 1599, 1753, 2166, 2260
BstFNI CGCG 1 cut(s) 442
BstH2I RGCGCY 2 cut(s) 347, 2131
BstHHI GCGC 4 cut(s) 54, 346, 444, 2130
BstKTI GATC 8 cut(s) 436, 478, 548, 714, 744, 958, 1855, 2148
BstMAI GTCTC 3 cut(s) 1139, 1535, 1713
BstMBI GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
BstMCI CGRYCG 1 cut(s) 778
BstMWI GCNNNNNNNGC 5 cut(s) 318, 337, 1769, 1924, 2093
BstNI CCWGG 2 cut(s) 626, 947
BstNSI RCATGY 2 cut(s) 1402, 1691
BstPAI GACNNNNGTC 2 cut(s) 1156, 2196
BstSCI CCNGG 2 cut(s) 624, 945
BstSFI CTRYAG 2 cut(s) 1656, 1900
BstUI CGCG 1 cut(s) 442
BstX2I RGATCY 2 cut(s) 711, 1852
BstXI CCANNNNNNTGG 1 cut(s) 1632
BstYI RGATCY 2 cut(s) 711, 1852
BstZ17I GTATAC 1 cut(s) 1186
BsuRI GGCC 3 cut(s) 1102, 2165, 2229
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 6 cut(s) 126, 1414, 1599, 1753, 2166, 2260
BtsI GCAGTG 2 cut(s) 116, 2255
BtsIMutI CAGTG 5 cut(s) 77, 116, 644, 1005, 2255
BveI ACCTGC 2 cut(s) 903, 1058
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 4 cut(s) 54, 346, 444, 2130
CseI GACGC 2 cut(s) 224, 2201
Csp6I GTAC 6 cut(s) 60, 102, 373, 1383, 1597, 1829
CviAII CATG 7 cut(s) 975, 1176, 1399, 1604, 1632, 1688, 1832
CviQI GTAC 6 cut(s) 60, 102, 373, 1383, 1597, 1829
DdeI CTNAG 8 cut(s) 721, 827, 1110, 1129, 1166, 1593, 1884, 2286
DpnI GATC 8 cut(s) 435, 477, 547, 713, 743, 957, 1854, 2147
DpnII GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
DraI TTTAAA 1 cut(s) 2109
DriI GACNNNNNGTC 1 cut(s) 1726
EaeI YGGCCR 1 cut(s) 2227
Eam1104I CTCTTC 1 cut(s) 382
Eam1105I GACNNNNNGTC 1 cut(s) 1726
EarI CTCTTC 1 cut(s) 382
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 2 cut(s) 1673, 1927
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 1102
Eco24I GRGCYC 2 cut(s) 1675, 1929
Eco31I GGTCTC 1 cut(s) 1139
Eco53kI GAGCTC 2 cut(s) 1673, 1927
Eco57I CTGAAG 3 cut(s) 101, 649, 1677
Eco88I CYCGRG 1 cut(s) 2043
EcoICRI GAGCTC 2 cut(s) 1673, 1927
EcoRI GAATTC 1 cut(s) 1466
EcoRII CCWGG 2 cut(s) 624, 945
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 1675, 1929
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 7 cut(s) 978, 1179, 1402, 1607, 1635, 1691, 1835
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 1539, 1571
FaqI GGGAC 1 cut(s) 698
FatI CATG 7 cut(s) 974, 1175, 1398, 1603, 1631, 1687, 1831
FauNDI CATATG 1 cut(s) 806
FbaI TGATCA 2 cut(s) 475, 741
FblI GTMKAC 6 cut(s) 238, 771, 1185, 1334, 1446, 1899
FokI GGATG 6 cut(s) 113, 1421, 1586, 1760, 2153, 2267
FriOI GRGCYC 2 cut(s) 1675, 1929
FspBI CTAG 4 cut(s) 11, 1259, 1538, 2268
GlaI GCGC 4 cut(s) 53, 345, 443, 2129
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 2 cut(s) 347, 2131
HaeIII GGCC 3 cut(s) 1102, 2165, 2229
HapII CCGG 2 cut(s) 529, 778
HgaI GACGC 2 cut(s) 224, 2201
HhaI GCGC 4 cut(s) 54, 346, 444, 2130
Hin1I GRCGYC 4 cut(s) 151, 235, 687, 1896
Hin1II CATG 7 cut(s) 978, 1179, 1402, 1607, 1635, 1691, 1835
Hin6I GCGC 4 cut(s) 52, 344, 442, 2128
HinP1I GCGC 4 cut(s) 52, 344, 442, 2128
HincII GTYRAC 5 cut(s) 465, 631, 763, 2201, 2283
HindII GTYRAC 5 cut(s) 465, 631, 763, 2201, 2283
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 7 cut(s) 410, 794, 1299, 1639, 1981, 2018, 2241
HpaII CCGG 2 cut(s) 529, 778
HphI GGTGA 3 cut(s) 66, 962, 1292
Hpy99I CGWCG 2 cut(s) 692, 2195
HpyAV CCTTC 4 cut(s) 213, 875, 1958, 2051
HpyCH4III ACNGT 7 cut(s) 101, 286, 389, 639, 1009, 1155, 1387
HpyCH4IV ACGT 8 cut(s) 21, 151, 687, 1219, 1290, 1827, 1896, 2259
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 337, 1769, 1924, 2093
HpyF3I CTNAG 8 cut(s) 721, 827, 1110, 1129, 1166, 1593, 1884, 2286
HpySE526I ACGT 8 cut(s) 21, 151, 687, 1219, 1290, 1827, 1896, 2259
Hsp92I GRCGYC 4 cut(s) 151, 235, 687, 1896
Hsp92II CATG 7 cut(s) 978, 1179, 1402, 1607, 1635, 1691, 1835
HspAI GCGC 4 cut(s) 52, 344, 442, 2128
Ksp22I TGATCA 2 cut(s) 475, 741
Kzo9I GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
LmnI GCTCC 4 cut(s) 116, 903, 1670, 2276
LweI GCATC 4 cut(s) 74, 135, 573, 2029
MaeI CTAG 4 cut(s) 11, 1259, 1538, 2268
MaeII ACGT 8 cut(s) 21, 151, 687, 1219, 1290, 1827, 1896, 2259
MaeIII GTNAC 4 cut(s) 304, 1323, 1892, 1948
MalI GATC 8 cut(s) 435, 477, 547, 713, 743, 957, 1854, 2147
MboI GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
MboII GAAGA 9 cut(s) 94, 369, 603, 1219, 1222, 1425, 1628, 2027, 2133
MflI RGATCY 2 cut(s) 711, 1852
MhlI GDGCHC 3 cut(s) 461, 1675, 1929
MlsI TGGCCA 1 cut(s) 2229
MluNI TGGCCA 1 cut(s) 2229
MlyI GAGTC 2 cut(s) 788, 1990
MmeI TCCRAC 1 cut(s) 2139
Mox20I TGGCCA 1 cut(s) 2229
MroXI GAANNNNTTC 2 cut(s) 224, 1620
MscI TGGCCA 1 cut(s) 2229
MseI TTAA 6 cut(s) 716, 960, 1215, 1287, 1428, 2108
MslI CAYNNNNRTG 4 cut(s) 109, 805, 1403, 1630
Msp20I TGGCCA 1 cut(s) 2229
MspCI CTTAAG 1 cut(s) 715
MspI CCGG 2 cut(s) 529, 778
MspR9I CCNGG 2 cut(s) 626, 947
Mva1269I GAATGC 2 cut(s) 200, 929
MvaI CCWGG 2 cut(s) 626, 947
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 5 cut(s) 318, 337, 1769, 1924, 2093
NdeI CATATG 1 cut(s) 806
NdeII GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
NlaIII CATG 7 cut(s) 978, 1179, 1402, 1607, 1635, 1691, 1835
NlaIV GGNNCC 3 cut(s) 294, 785, 1854
NmuCI GTSAC 1 cut(s) 1892
NspI RCATGY 2 cut(s) 1402, 1691
NspV TTCGAA 1 cut(s) 186
OliI CACNNNNGTG 1 cut(s) 109
PaeR7I CTCGAG 1 cut(s) 2043
PceI AGGCCT 1 cut(s) 1102
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 2 cut(s) 200, 929
PdmI GAANNNNTTC 2 cut(s) 224, 1620
PfeI GAWTC 5 cut(s) 410, 1299, 1639, 2018, 2241
PleI GAGTC 2 cut(s) 788, 1989
PpsI GAGTC 2 cut(s) 788, 1989
PshAI GACNNNNGTC 2 cut(s) 1156, 2196
PsiI TTATAA 2 cut(s) 519, 1392
Psp124BI GAGCTC 2 cut(s) 1675, 1929
Psp1406I AACGTT 1 cut(s) 1290
Psp6I CCWGG 2 cut(s) 624, 945
PspGI CCWGG 2 cut(s) 624, 945
PspN4I GGNNCC 3 cut(s) 294, 785, 1854
PspXI VCTCGAGB 1 cut(s) 2043
PsuI RGATCY 2 cut(s) 711, 1852
RsaI GTAC 6 cut(s) 61, 103, 374, 1384, 1598, 1830
RsaNI GTAC 6 cut(s) 60, 102, 373, 1383, 1597, 1829
RseI CAYNNNNRTG 4 cut(s) 109, 805, 1403, 1630
SacI GAGCTC 2 cut(s) 1675, 1929
SaqAI TTAA 6 cut(s) 716, 960, 1215, 1287, 1428, 2108
Sau3AI GATC 8 cut(s) 433, 475, 545, 711, 741, 955, 1852, 2145
SchI GAGTC 2 cut(s) 788, 1990
ScrFI CCNGG 2 cut(s) 626, 947
SduI GDGCHC 3 cut(s) 461, 1675, 1929
SfaNI GCATC 4 cut(s) 74, 135, 573, 2029
SfcI CTRYAG 2 cut(s) 1656, 1900
Sfr274I CTCGAG 1 cut(s) 2043
SfuI TTCGAA 1 cut(s) 186
SlaI CTCGAG 1 cut(s) 2043
SmiMI CAYNNNNRTG 4 cut(s) 109, 805, 1403, 1630
SmlI CTYRAG 5 cut(s) 229, 313, 715, 1115, 2043
SmoI CTYRAG 5 cut(s) 229, 313, 715, 1115, 2043
SseBI AGGCCT 1 cut(s) 1102
SsiI CCGC 4 cut(s) 107, 114, 338, 492
SspI AATATT 1 cut(s) 259
SspMI CTAG 4 cut(s) 11, 1259, 1538, 2268
SstI GAGCTC 2 cut(s) 1675, 1929
StuI AGGCCT 1 cut(s) 1102
StyD4I CCNGG 2 cut(s) 624, 945
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 7 cut(s) 101, 286, 389, 639, 1009, 1155, 1387
TaiI ACGT 8 cut(s) 24, 154, 690, 1222, 1293, 1830, 1899, 2262
TaqI TCGA 5 cut(s) 186, 910, 2044, 2239, 2244
TaqII GACCGA 1 cut(s) 1628
TatI WGTACW 4 cut(s) 59, 101, 372, 1596
TfiI GAWTC 5 cut(s) 410, 1299, 1639, 2018, 2241
Tru1I TTAA 6 cut(s) 716, 960, 1215, 1287, 1428, 2108
Tru9I TTAA 6 cut(s) 716, 960, 1215, 1287, 1428, 2108
TscAI CASTG 5 cut(s) 84, 116, 644, 1012, 2255
TseFI GTSAC 1 cut(s) 1892
Tsp45I GTSAC 1 cut(s) 1892
TspDTI ATGAA 8 cut(s) 19, 402, 597, 663, 894, 936, 1459, 2070
TspGWI ACGGA 3 cut(s) 617, 858, 930
TspRI CASTG 5 cut(s) 84, 116, 644, 1012, 2255
Vha464I CTTAAG 1 cut(s) 715
XapI RAATTY 4 cut(s) 1466, 1619, 2110, 2222
XbaI TCTAGA 1 cut(s) 1537
XceI RCATGY 2 cut(s) 1402, 1691
XhoI CTCGAG 1 cut(s) 2043
XmiI GTMKAC 6 cut(s) 238, 771, 1185, 1334, 1446, 1899
XmnI GAANNNNTTC 2 cut(s) 224, 1620
XspI CTAG 4 cut(s) 11, 1259, 1538, 2268
ZraI GACGTC 3 cut(s) 152, 688, 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.