wall-associated receptor kinase-like
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1307 |
| Acc36I | ACCTGC | 2 cut(s) | 420, 956 |
| AccI | GTMKAC | 5 cut(s) | 188, 1224, 1271, 1291, 1565 |
| AciI | CCGC | 5 cut(s) | 315, 369, 381, 799, 1963 |
| AclWI | GGATC | 3 cut(s) | 137, 1413, 1426 |
| AcoI | YGGCCR | 2 cut(s) | 571, 1210 |
| AcuI | CTGAAG | 1 cut(s) | 245 |
| AfaI | GTAC | 7 cut(s) | 28, 119, 231, 344, 487, 874, 1020 |
| AfeI | AGCGCT | 1 cut(s) | 63 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 2165 |
| AflII | CTTAAG | 1 cut(s) | 1480 |
| AgeI | ACCGGT | 1 cut(s) | 455 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1561 |
| AhlI | ACTAGT | 1 cut(s) | 1915 |
| AjnI | CCWGG | 1 cut(s) | 615 |
| AleI | CACNNNNGTG | 1 cut(s) | 1465 |
| AluBI | AGCT | 5 cut(s) | 21, 402, 1532, 1740, 1993 |
| AluI | AGCT | 5 cut(s) | 21, 402, 1532, 1740, 1993 |
| Alw21I | GWGCWC | 1 cut(s) | 404 |
| Alw26I | GTCTC | 5 cut(s) | 346, 394, 632, 1370, 1548 |
| AlwI | GGATC | 3 cut(s) | 137, 1413, 1426 |
| Ama87I | CYCGRG | 2 cut(s) | 976, 1878 |
| Aor51HI | AGCGCT | 1 cut(s) | 63 |
| AoxI | GGCC | 4 cut(s) | 134, 571, 887, 1210 |
| ApeKI | GCWGC | 9 cut(s) | 44, 64, 371, 801, 894, 1007, 1444, 1529, 2138 |
| AsiGI | ACCGGT | 1 cut(s) | 455 |
| AspLEI | GCGC | 3 cut(s) | 64, 659, 1479 |
| AspS9I | GGNCC | 6 cut(s) | 134, 554, 888, 1687, 1750, 2144 |
| AsuC2I | CCSGG | 2 cut(s) | 891, 915 |
| AsuHPI | GGTGA | 7 cut(s) | 277, 358, 409, 1042, 1127, 2042, 2148 |
| AsuNHI | GCTAGC | 1 cut(s) | 536 |
| AvaI | CYCGRG | 2 cut(s) | 976, 1878 |
| AvaII | GGWCC | 4 cut(s) | 554, 1687, 1750, 2144 |
| BalI | TGGCCA | 1 cut(s) | 1212 |
| BamHI | GGATCC | 1 cut(s) | 1418 |
| BanII | GRGCYC | 2 cut(s) | 404, 1510 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1896, 1928 |
| BauI | CACGAG | 1 cut(s) | 1448 |
| BbsI | GAAGAC | 1 cut(s) | 2142 |
| Bbv12I | GWGCWC | 1 cut(s) | 404 |
| BbvI | GCAGC | 9 cut(s) | 51, 56, 383, 788, 906, 994, 1456, 1541, 2125 |
| BccI | CCATC | 6 cut(s) | 464, 545, 812, 863, 1238, 2167 |
| BceAI | ACGGC | 1 cut(s) | 268 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1835, 1869 |
| BciT130I | CCWGG | 1 cut(s) | 617 |
| BciVI | GTATCC | 1 cut(s) | 937 |
| BcnI | CCSGG | 2 cut(s) | 891, 915 |
| BcoDI | GTCTC | 5 cut(s) | 346, 394, 632, 1370, 1548 |
| BcuI | ACTAGT | 1 cut(s) | 1915 |
| BfaI | CTAG | 6 cut(s) | 192, 537, 692, 1580, 1623, 1916 |
| BfmI | CTRYAG | 1 cut(s) | 652 |
| BfoI | RGCGCY | 2 cut(s) | 65, 660 |
| BfrI | CTTAAG | 1 cut(s) | 1480 |
| BfuAI | ACCTGC | 2 cut(s) | 420, 956 |
| BfuI | GTATCC | 1 cut(s) | 937 |
| BmcAI | AGTACT | 1 cut(s) | 28 |
| Bme1390I | CCNGG | 3 cut(s) | 617, 891, 915 |
| Bme18I | GGWCC | 4 cut(s) | 554, 1687, 1750, 2144 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1561 |
| BmeT110I | CYCGRG | 2 cut(s) | 976, 1878 |
| BmgT120I | GGNCC | 6 cut(s) | 134, 554, 888, 1687, 1750, 2144 |
| BmiI | GGNNCC | 5 cut(s) | 1386, 1420, 1507, 1689, 1961 |
| BmrFI | CCNGG | 3 cut(s) | 617, 891, 915 |
| BmsI | GCATC | 6 cut(s) | 120, 653, 872, 1833, 1864, 1954 |
| BmtI | GCTAGC | 1 cut(s) | 540 |
| BpiI | GAAGAC | 1 cut(s) | 2142 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 496, 528 |
| BpmI | CTGGAG | 1 cut(s) | 2015 |
| BpuEI | CTTGAG | 4 cut(s) | 43, 229, 1148, 1786 |
| BpuMI | CCSGG | 2 cut(s) | 891, 915 |
| BsaAI | YACGTR | 1 cut(s) | 1467 |
| BsaBI | GATNNNNATC | 1 cut(s) | 822 |
| BsaJI | CCNNGG | 5 cut(s) | 393, 1198, 1213, 1491, 2081 |
| BsaWI | WCCGGW | 2 cut(s) | 326, 455 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 10, 40 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 2165 |
| Bse118I | RCCGGY | 2 cut(s) | 455, 569 |
| Bse1I | ACTGG | 3 cut(s) | 184, 1032, 1702 |
| Bse8I | GATNNNNATC | 1 cut(s) | 822 |
| BseBI | CCWGG | 1 cut(s) | 617 |
| BseDI | CCNNGG | 5 cut(s) | 393, 1198, 1213, 1491, 2081 |
| BseGI | GGATG | 4 cut(s) | 111, 556, 1855, 2092 |
| BseJI | GATNNNNATC | 1 cut(s) | 822 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 2165 |
| BseMII | CTCAG | 4 cut(s) | 224, 362, 417, 535 |
| BseNI | ACTGG | 3 cut(s) | 184, 1032, 1702 |
| BseX3I | CGGCCG | 1 cut(s) | 571 |
| BseXI | GCAGC | 9 cut(s) | 51, 56, 383, 788, 906, 994, 1456, 1541, 2125 |
| BsgI | GTGCAG | 2 cut(s) | 448, 1548 |
| Bsh1285I | CGRYCG | 1 cut(s) | 574 |
| BshFI | GGCC | 4 cut(s) | 136, 573, 889, 1212 |
| BshTI | ACCGGT | 1 cut(s) | 455 |
| BsiEI | CGRYCG | 1 cut(s) | 574 |
| BsiHKAI | GWGCWC | 1 cut(s) | 404 |
| BsiHKCI | CYCGRG | 2 cut(s) | 976, 1878 |
| BsiSI | CCGG | 6 cut(s) | 327, 456, 570, 593, 891, 914 |
| BslFI | GGGAC | 3 cut(s) | 1231, 1684, 2122 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 2165 |
| BsmAI | GTCTC | 5 cut(s) | 346, 394, 632, 1370, 1548 |
| BsmFI | GGGAC | 3 cut(s) | 1231, 1684, 2122 |
| BsmI | GAATGC | 1 cut(s) | 611 |
| BsnI | GGCC | 4 cut(s) | 136, 573, 889, 1212 |
| BsoBI | CYCGRG | 2 cut(s) | 976, 1878 |
| Bsp1286I | GDGCHC | 2 cut(s) | 404, 1510 |
| Bsp143I | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| Bsp19I | CCATGG | 1 cut(s) | 2081 |
| BspACI | CCGC | 5 cut(s) | 315, 369, 381, 799, 1963 |
| BspANI | GGCC | 4 cut(s) | 136, 573, 889, 1212 |
| BspCNI | CTCAG | 4 cut(s) | 225, 361, 416, 534 |
| BspLI | GGNNCC | 5 cut(s) | 1386, 1420, 1507, 1689, 1961 |
| BspMI | ACCTGC | 2 cut(s) | 420, 956 |
| BspOI | GCTAGC | 1 cut(s) | 540 |
| BspPI | GGATC | 3 cut(s) | 137, 1413, 1426 |
| BspTI | CTTAAG | 1 cut(s) | 1480 |
| BsrFI | RCCGGY | 2 cut(s) | 455, 569 |
| BsrI | ACTGG | 3 cut(s) | 184, 1032, 1702 |
| BssAI | RCCGGY | 2 cut(s) | 455, 569 |
| BssECI | CCNNGG | 5 cut(s) | 393, 1198, 1213, 1491, 2081 |
| BssMI | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| BssSI | CACGAG | 1 cut(s) | 1448 |
| BssT1I | CCWWGG | 4 cut(s) | 393, 1198, 1213, 2081 |
| Bst2BI | CACGAG | 1 cut(s) | 1448 |
| Bst2UI | CCWGG | 1 cut(s) | 617 |
| Bst4CI | ACNGT | 8 cut(s) | 584, 990, 1104, 1222, 1302, 1526, 2113, 2178 |
| Bst6I | CTCTTC | 2 cut(s) | 153, 2040 |
| BstAFI | CTTAAG | 1 cut(s) | 1480 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 900, 1604 |
| BstBAI | YACGTR | 1 cut(s) | 1467 |
| BstC8I | GCNNGC | 5 cut(s) | 257, 311, 315, 538, 887 |
| BstDEI | CTNAG | 4 cut(s) | 233, 348, 403, 521 |
| BstDSI | CCRYGG | 2 cut(s) | 1491, 2081 |
| BstF5I | GGATG | 4 cut(s) | 111, 556, 1855, 2092 |
| BstH2I | RGCGCY | 2 cut(s) | 65, 660 |
| BstHHI | GCGC | 3 cut(s) | 64, 659, 1479 |
| BstKTI | GATC | 6 cut(s) | 132, 409, 1421, 1628, 1799, 1855 |
| BstMAI | GTCTC | 5 cut(s) | 346, 394, 632, 1370, 1548 |
| BstMBI | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| BstMCI | CGRYCG | 1 cut(s) | 574 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 44, 891, 900, 1053, 1209, 1604, 1928 |
| BstNI | CCWGG | 1 cut(s) | 617 |
| BstSCI | CCNGG | 3 cut(s) | 615, 889, 913 |
| BstSFI | CTRYAG | 1 cut(s) | 652 |
| BstV1I | GCAGC | 9 cut(s) | 51, 56, 383, 788, 906, 994, 1456, 1541, 2125 |
| BstV2I | GAAGAC | 1 cut(s) | 2142 |
| BstX2I | RGATCY | 1 cut(s) | 1418 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1467 |
| BstYI | RGATCY | 1 cut(s) | 1418 |
| BstZI | CGGCCG | 1 cut(s) | 571 |
| BsuI | GTATCC | 1 cut(s) | 937 |
| BsuRI | GGCC | 4 cut(s) | 136, 573, 889, 1212 |
| BtgI | CCRYGG | 2 cut(s) | 1491, 2081 |
| BtgZI | GCGATG | 1 cut(s) | 2145 |
| BtsCI | GGATG | 4 cut(s) | 111, 556, 1855, 2092 |
| BtsI | GCAGTG | 1 cut(s) | 531 |
| BtsIMutI | CAGTG | 5 cut(s) | 531, 589, 792, 1170, 2174 |
| BveI | ACCTGC | 2 cut(s) | 420, 956 |
| Cac8I | GCNNGC | 5 cut(s) | 257, 311, 315, 538, 887 |
| CfoI | GCGC | 3 cut(s) | 64, 659, 1479 |
| Cfr10I | RCCGGY | 2 cut(s) | 455, 569 |
| Cfr13I | GGNCC | 6 cut(s) | 134, 554, 888, 1687, 1750, 2144 |
| Csp6I | GTAC | 7 cut(s) | 27, 118, 230, 343, 486, 873, 1019 |
| CspAI | ACCGGT | 1 cut(s) | 455 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1480, 1515 |
| CviAII | CATG | 7 cut(s) | 565, 677, 904, 1011, 1184, 1439, 2082 |
| CviQI | GTAC | 7 cut(s) | 27, 118, 230, 343, 486, 873, 1019 |
| DdeI | CTNAG | 4 cut(s) | 233, 348, 403, 521 |
| DpnI | GATC | 6 cut(s) | 131, 408, 1420, 1627, 1798, 1854 |
| DpnII | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1561 |
| EaeI | YGGCCR | 2 cut(s) | 571, 1210 |
| EagI | CGGCCG | 1 cut(s) | 571 |
| Eam1104I | CTCTTC | 2 cut(s) | 153, 2040 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1561 |
| EarI | CTCTTC | 2 cut(s) | 153, 2040 |
| Ecl136II | GAGCTC | 1 cut(s) | 402 |
| EclXI | CGGCCG | 1 cut(s) | 571 |
| Eco130I | CCWWGG | 4 cut(s) | 393, 1198, 1213, 2081 |
| Eco24I | GRGCYC | 2 cut(s) | 404, 1510 |
| Eco32I | GATATC | 1 cut(s) | 1909 |
| Eco47I | GGWCC | 4 cut(s) | 554, 1687, 1750, 2144 |
| Eco47III | AGCGCT | 1 cut(s) | 63 |
| Eco52I | CGGCCG | 1 cut(s) | 571 |
| Eco53kI | GAGCTC | 1 cut(s) | 402 |
| Eco57I | CTGAAG | 1 cut(s) | 245 |
| Eco88I | CYCGRG | 2 cut(s) | 976, 1878 |
| EcoICRI | GAGCTC | 1 cut(s) | 402 |
| EcoRII | CCWGG | 1 cut(s) | 615 |
| EcoRV | GATATC | 1 cut(s) | 1909 |
| EcoT14I | CCWWGG | 4 cut(s) | 393, 1198, 1213, 2081 |
| EcoT38I | GRGCYC | 2 cut(s) | 404, 1510 |
| ErhI | CCWWGG | 4 cut(s) | 393, 1198, 1213, 2081 |
| FaeI | CATG | 7 cut(s) | 568, 680, 907, 1014, 1187, 1442, 2085 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 191, 223, 1110, 1142, 1278, 1310 |
| FaqI | GGGAC | 3 cut(s) | 1231, 1684, 2122 |
| FatI | CATG | 7 cut(s) | 564, 676, 903, 1010, 1183, 1438, 2081 |
| FauI | CCCGC | 1 cut(s) | 322 |
| FblI | GTMKAC | 5 cut(s) | 188, 1224, 1271, 1291, 1565 |
| FokI | GGATG | 4 cut(s) | 98, 563, 1862, 2099 |
| FriOI | GRGCYC | 2 cut(s) | 404, 1510 |
| FspBI | CTAG | 6 cut(s) | 192, 537, 692, 1580, 1623, 1916 |
| GlaI | GCGC | 3 cut(s) | 63, 658, 1478 |
| GsuI | CTGGAG | 1 cut(s) | 2015 |
| HaeII | RGCGCY | 2 cut(s) | 65, 660 |
| HaeIII | GGCC | 4 cut(s) | 136, 573, 889, 1212 |
| HapII | CCGG | 6 cut(s) | 327, 456, 570, 593, 891, 914 |
| HhaI | GCGC | 3 cut(s) | 64, 659, 1479 |
| Hin1II | CATG | 7 cut(s) | 568, 680, 907, 1014, 1187, 1442, 2085 |
| Hin6I | GCGC | 3 cut(s) | 62, 657, 1477 |
| HinP1I | GCGC | 3 cut(s) | 62, 657, 1477 |
| HincII | GTYRAC | 1 cut(s) | 1566 |
| HindII | GTYRAC | 1 cut(s) | 1566 |
| HinfI | GANTC | 9 cut(s) | 50, 363, 979, 1194, 1251, 1708, 1816, 2002, 2069 |
| HpaII | CCGG | 6 cut(s) | 327, 456, 570, 593, 891, 914 |
| HphI | GGTGA | 7 cut(s) | 277, 358, 409, 1042, 1127, 2042, 2148 |
| Hpy188I | TCNGA | 7 cut(s) | 324, 351, 406, 469, 1413, 1815, 1950 |
| Hpy188III | TCNNGA | 7 cut(s) | 54, 127, 208, 848, 1127, 1623, 1765 |
| Hpy99I | CGWCG | 1 cut(s) | 727 |
| HpyAV | CCTTC | 3 cut(s) | 920, 1280, 1802 |
| HpyCH4III | ACNGT | 8 cut(s) | 584, 990, 1104, 1222, 1302, 1526, 2113, 2178 |
| HpyCH4IV | ACGT | 2 cut(s) | 294, 1466 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 44, 891, 900, 1053, 1209, 1604, 1928 |
| HpyF3I | CTNAG | 4 cut(s) | 233, 348, 403, 521 |
| HpySE526I | ACGT | 2 cut(s) | 294, 1466 |
| Hsp92II | CATG | 7 cut(s) | 568, 680, 907, 1014, 1187, 1442, 2085 |
| HspAI | GCGC | 3 cut(s) | 62, 657, 1477 |
| Kzo9I | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| LmnI | GCTCC | 3 cut(s) | 18, 678, 1505 |
| Lsp1109I | GCAGC | 9 cut(s) | 51, 56, 383, 788, 906, 994, 1456, 1541, 2125 |
| LweI | GCATC | 6 cut(s) | 120, 653, 872, 1833, 1864, 1954 |
| MaeI | CTAG | 6 cut(s) | 192, 537, 692, 1580, 1623, 1916 |
| MaeII | ACGT | 2 cut(s) | 294, 1466 |
| MaeIII | GTNAC | 9 cut(s) | 277, 295, 529, 602, 1679, 1789, 1981, 2010, 2113 |
| MalI | GATC | 6 cut(s) | 131, 408, 1420, 1627, 1798, 1854 |
| MboI | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| MfeI | CAATTG | 1 cut(s) | 2073 |
| MflI | RGATCY | 1 cut(s) | 1418 |
| MhlI | GDGCHC | 2 cut(s) | 404, 1510 |
| MlsI | TGGCCA | 1 cut(s) | 1212 |
| MluNI | TGGCCA | 1 cut(s) | 1212 |
| MlyI | GAGTC | 3 cut(s) | 988, 1717, 1825 |
| MmeI | TCCRAC | 3 cut(s) | 862, 1140, 1793 |
| Mox20I | TGGCCA | 1 cut(s) | 1212 |
| MscI | TGGCCA | 1 cut(s) | 1212 |
| MseI | TTAA | 7 cut(s) | 777, 951, 1050, 1122, 1137, 1353, 1481 |
| MslI | CAYNNNNRTG | 4 cut(s) | 694, 1344, 1465, 1950 |
| Msp20I | TGGCCA | 1 cut(s) | 1212 |
| MspA1I | CMGCKG | 1 cut(s) | 801 |
| MspCI | CTTAAG | 1 cut(s) | 1480 |
| MspI | CCGG | 6 cut(s) | 327, 456, 570, 593, 891, 914 |
| MspR9I | CCNGG | 3 cut(s) | 617, 891, 915 |
| MunI | CAATTG | 1 cut(s) | 2073 |
| Mva1269I | GAATGC | 1 cut(s) | 611 |
| MvaI | CCWGG | 1 cut(s) | 617 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 44, 891, 900, 1053, 1209, 1604, 1928 |
| NciI | CCSGG | 2 cut(s) | 891, 915 |
| NcoI | CCATGG | 1 cut(s) | 2081 |
| NdeII | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| NheI | GCTAGC | 1 cut(s) | 536 |
| NlaIII | CATG | 7 cut(s) | 568, 680, 907, 1014, 1187, 1442, 2085 |
| NlaIV | GGNNCC | 5 cut(s) | 1386, 1420, 1507, 1689, 1961 |
| NmuCI | GTSAC | 4 cut(s) | 529, 602, 1789, 2010 |
| OliI | CACNNNNGTG | 1 cut(s) | 1465 |
| PaeR7I | CTCGAG | 2 cut(s) | 976, 1878 |
| PctI | GAATGC | 1 cut(s) | 611 |
| PfeI | GAWTC | 6 cut(s) | 50, 363, 1194, 1251, 2002, 2069 |
| PinAI | ACCGGT | 1 cut(s) | 455 |
| PleI | GAGTC | 3 cut(s) | 987, 1716, 1824 |
| PpsI | GAGTC | 3 cut(s) | 987, 1716, 1824 |
| Ppu21I | YACGTR | 1 cut(s) | 1467 |
| PsiI | TTATAA | 1 cut(s) | 1307 |
| Psp124BI | GAGCTC | 1 cut(s) | 404 |
| Psp6I | CCWGG | 1 cut(s) | 615 |
| PspGI | CCWGG | 1 cut(s) | 615 |
| PspN4I | GGNNCC | 5 cut(s) | 1386, 1420, 1507, 1689, 1961 |
| PspPI | GGNCC | 6 cut(s) | 134, 554, 888, 1687, 1750, 2144 |
| PspXI | VCTCGAGB | 2 cut(s) | 976, 1878 |
| PsuI | RGATCY | 1 cut(s) | 1418 |
| RsaI | GTAC | 7 cut(s) | 28, 119, 231, 344, 487, 874, 1020 |
| RsaNI | GTAC | 7 cut(s) | 27, 118, 230, 343, 486, 873, 1019 |
| RseI | CAYNNNNRTG | 4 cut(s) | 694, 1344, 1465, 1950 |
| SacI | GAGCTC | 1 cut(s) | 404 |
| SalI | GTCGAC | 1 cut(s) | 1564 |
| SaqAI | TTAA | 7 cut(s) | 777, 951, 1050, 1122, 1137, 1353, 1481 |
| Sau3AI | GATC | 6 cut(s) | 129, 406, 1418, 1625, 1796, 1852 |
| Sau96I | GGNCC | 6 cut(s) | 134, 554, 888, 1687, 1750, 2144 |
| ScaI | AGTACT | 1 cut(s) | 28 |
| SchI | GAGTC | 3 cut(s) | 988, 1717, 1825 |
| ScrFI | CCNGG | 3 cut(s) | 617, 891, 915 |
| SduI | GDGCHC | 2 cut(s) | 404, 1510 |
| SfaNI | GCATC | 6 cut(s) | 120, 653, 872, 1833, 1864, 1954 |
| SfcI | CTRYAG | 1 cut(s) | 652 |
| Sfr274I | CTCGAG | 2 cut(s) | 976, 1878 |
| SinI | GGWCC | 4 cut(s) | 554, 1687, 1750, 2144 |
| SlaI | CTCGAG | 2 cut(s) | 976, 1878 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 694, 1344, 1465, 1950 |
| SmlI | CTYRAG | 7 cut(s) | 22, 208, 976, 1127, 1480, 1765, 1878 |
| SmoI | CTYRAG | 7 cut(s) | 22, 208, 976, 1127, 1480, 1765, 1878 |
| SpeI | ACTAGT | 1 cut(s) | 1915 |
| SsiI | CCGC | 5 cut(s) | 315, 369, 381, 799, 1963 |
| SspMI | CTAG | 6 cut(s) | 192, 537, 692, 1580, 1623, 1916 |
| SstI | GAGCTC | 1 cut(s) | 404 |
| StyD4I | CCNGG | 3 cut(s) | 615, 889, 913 |
| StyI | CCWWGG | 4 cut(s) | 393, 1198, 1213, 2081 |
| TaaI | ACNGT | 8 cut(s) | 584, 990, 1104, 1222, 1302, 1526, 2113, 2178 |
| TaiI | ACGT | 2 cut(s) | 297, 1469 |
| TaqI | TCGA | 5 cut(s) | 816, 977, 1565, 1706, 1879 |
| TaqII | GACCGA | 1 cut(s) | 771 |
| TatI | WGTACW | 2 cut(s) | 26, 342 |
| TauI | GCSGC | 2 cut(s) | 384, 801 |
| TfiI | GAWTC | 6 cut(s) | 50, 363, 1194, 1251, 2002, 2069 |
| Tru1I | TTAA | 7 cut(s) | 777, 951, 1050, 1122, 1137, 1353, 1481 |
| Tru9I | TTAA | 7 cut(s) | 777, 951, 1050, 1122, 1137, 1353, 1481 |
| TscAI | CASTG | 5 cut(s) | 538, 589, 792, 1177, 2181 |
| TseFI | GTSAC | 4 cut(s) | 529, 602, 1789, 2010 |
| TseI | GCWGC | 9 cut(s) | 44, 64, 371, 801, 894, 1007, 1444, 1529, 2138 |
| Tsp45I | GTSAC | 4 cut(s) | 529, 602, 1789, 2010 |
| TspDTI | ATGAA | 8 cut(s) | 920, 1172, 1538, 1599, 1822, 1905, 2058, 2142 |
| TspGWI | ACGGA | 5 cut(s) | 504, 1095, 1508, 1733, 2136 |
| TspRI | CASTG | 5 cut(s) | 538, 589, 792, 1177, 2181 |
| Vha464I | CTTAAG | 1 cut(s) | 1480 |
| VpaK11BI | GGWCC | 4 cut(s) | 554, 1687, 1750, 2144 |
| XbaI | TCTAGA | 1 cut(s) | 1622 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 95 |
| XhoI | CTCGAG | 2 cut(s) | 976, 1878 |
| XmiI | GTMKAC | 5 cut(s) | 188, 1224, 1271, 1291, 1565 |
| XspI | CTAG | 6 cut(s) | 192, 537, 692, 1580, 1623, 1916 |
| ZrmI | AGTACT | 1 cut(s) | 28 |