Rroxscaffold_2G00095490

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000052
Physical Location & Seq
Forward (+)
16851432 .. 16855015
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095490.1

Sequence Viewer

Length: 2217 bp
ATGGATGTTGTGCAGCTTTTACTTCATATTACTCTGTTCTTGTGGTATGTTAGTATTAATACTGCTACAGTAGCAGCAGCGCCGCAACAAATCTTAATAGCAAAGCCTAATTGTCCATCACATTGTGGAAACATTTCAATCCCATATCCTTTTGGAATTGGACCAGGTTGTTACGCAGATAACTGGTTCGAAATACTGTGTAATGAGTCAGTCAGCCCACCCAAACCTTTCTTGAACCGCACCACTCTGAATCTTGAGGTACTGGAAATTTCTATTGCAGGAACACTAAAAGTCAGAAACCCGATTACGGTCTCTAATAATTGCAGTGATAAGCCAATTCGGCAAGCAGCCTACTTGTGGGGAAGTCCTTTTGTGTTCTCGCAAAAGAACAGATTCACAGTAGTTGGTTGTGGTGTTATGGCTGGGCTGAATTCTAGCTCAGATGGCTTGACTATCTCGGCTACGTGCTTGTCCCAGTGTGAAATTGCCTCCAATGCAAGCAGTACCTGCAATGGTTTGGATTGCAGCCAGGTCTCTATCCCTTCCTTTCTCTCTACCTTCGATACCAGTTTCATATCTACAACAAATCCATGCAATTATGCATTCTTGGCAGACCAGGCCTGGTTTCAGTCGTTTACCAACAATTTAACTAATATCTCTGCCAGAATTAGTGATATGGATGATGTGCCTGCCGTGTTGGAATGGAGCTTATATCATTCGATCCTCGATGTATTTGGAACCTCTTCAATCGCTTCTGATAGATCTGTTAACTGCCTGGAATACAATGACACCTCTTCAACATATAGTTCCTCAAGGCTGGAATGTTTCTGTGCAACGGGCTTTGAAGGAAACCCCTATCTCATTGAAGGATGTCAAGATATAAATGAATGCTTGGCTCCAAACCGGCAGTGTCCTGTGGACTATGCTTGTAAGAATCATCCTGGATATGCCGAATGCTACTATCCAAAGAGAAAGTCCGCAGTTAAACTGGCCTTTATAGTTATTGGTAGCGTTCTTGGACTACTGTTTCTACTCGGTGTTGCTTGGTGGTTGCACAAAGCCATAAAGAAAAGGAAGAACATAAAGCAGAAGGAGAAGTTTTTTAAACAAAATGGTGGTTTATTGTTGGAGCAACAACTATTATCTGGTGAAGTAAATGTCGACAAAATTAAGTTGTTCAATTCGAAGGAGTTGGACAAAGCTACTGACCACTTTAATGTGGACAGAATTCTTGGCCAAGGAGGCCAAGGTACCGTTTACAAAGGAATGCTGGAAGATGGAAGAATTGTTGCTGTAAAGCAGTCTAAAAAACTTGTTGGAGGTGAAGTTGGAAATTTCATTAATGAAATTGTCATTCTTTCACAAATTAACCATAGGAATGTGGTCAAGTTATTGGGGTGCTGTTTAGAGACGGAAGTTCCTCTTTTGGTTTATGAATTCATACTTAATGGAACACTTTCTCAGTATATTCATCAACATAATGAGGAGTTTCCTCTCACTTGGGAAATGCGCTTACGGGTGTCGATGGAAATTGCAGAAGCTATGTCGTACTTACATTCATCTGCTTCCATGCCTATATACCACAGAGACATCAAGTCTTCTAACATACTATTGGATGATAAGTATAGAGCCAAAGTAGCAGACTTTGGAACATCAAGATCTGTAGCCATTGATAAGACACACCTTACTACACGAGTACAAGGAACATTTGGTTATCTGGACCCAGAATATTTCCAATCCAGTCAATTTACTGACAAGAGTGATGTTTATAGCTTTGGAGTGGTCCTTGCTGAGCTCTTGACAGGACAAAAACCAATTTCTCCGACAACCTCAGATGAATGGAGAAGCCTTGCAAACCATTTCATTCTTTCAATGGAAGGAAATTGCCTGTTCGATATTCTCGATGCTCGAGTTAGAAGTGATGGTCGGGAAGAAGAAATTGTGGCAGTTGCTAACCTTGCAAAAAGATGCTTAAATTTGAACGGGAGAAAACGACCTACTATGAAAGAAGTTGCTGTGGGGTTGGAGGGAATCCAATTGTCGGTTAAAGCTCAGTCTGATGCTCAACAAAACTTCTCAGAGATTGGGTATGATCGAACTAATGGAATGACCGAGGCTTGGGATGTTTGTTCTACATCAACAGGGTTATCATGTATGGATAGTACTACAGGTTCTTCATTAGATGCACAGCCTCTGCTGTCCTTTAAGTATGAGTGA

Protein Analysis

738

Amino Acids

81.68

Weight (kDa)

5.66

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 98 9.5e-12 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 405 - 671 2.1e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 407 - 672 2.9e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 1161
AciI CCGC 3 cut(s) 83, 238, 978
AclWI GGATC 1 cut(s) 715
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 6 cut(s) 267, 430, 1227, 1333, 1436, 1975
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 6 cut(s) 261, 505, 1252, 1550, 1698, 2164
AfiI CCNNNNNNNGG 4 cut(s) 307, 357, 2041, 2118
AgsI TTSAA 9 cut(s) 138, 235, 747, 798, 845, 866, 1180, 1872, 1981
AhdI GACNNNNNGTC 1 cut(s) 1594
AjnI CCWGG 6 cut(s) 163, 528, 615, 620, 774, 940
AluBI AGCT 8 cut(s) 16, 438, 708, 1202, 1541, 1773, 1795, 2051
AluI AGCT 8 cut(s) 16, 438, 708, 1202, 1541, 1773, 1795, 2051
Alw21I GWGCWC 1 cut(s) 1797
Alw26I GTCTC 4 cut(s) 316, 538, 1403, 1581
AlwI GGATC 1 cut(s) 715
AlwNI CAGNNNCTG 2 cut(s) 507, 2194
Ama87I CYCGRG 1 cut(s) 1908
AoxI GGCC 4 cut(s) 618, 990, 1234, 1243
ApeKI GCWGC 5 cut(s) 13, 74, 77, 347, 525
ApoI RAATTY 6 cut(s) 267, 430, 1227, 1333, 1436, 1975
ArsI GACNNNNNNTTYG 2 cut(s) 959, 991
AseI ATTAAT 2 cut(s) 57, 1341
Asp700I GAANNNNTTC 4 cut(s) 133, 392, 742, 1456
Asp718I GGTACC 1 cut(s) 1250
AspLEI GCGC 2 cut(s) 82, 1512
AspS9I GGNCC 3 cut(s) 161, 1720, 1783
AsuHPI GGTGA 2 cut(s) 1160, 1334
AsuII TTCGAA 2 cut(s) 189, 1184
AvaI CYCGRG 1 cut(s) 1908
AvaII GGWCC 3 cut(s) 161, 1720, 1783
BalI TGGCCA 1 cut(s) 1236
BanI GGYRCC 1 cut(s) 1250
BanII GRGCYC 1 cut(s) 1797
BauI CACGAG 1 cut(s) 1692
BbsI GAAGAC 1 cut(s) 1590
Bbv12I GWGCWC 1 cut(s) 1797
BbvI GCAGC 5 cut(s) 25, 86, 89, 359, 537
BccI CCATC 5 cut(s) 124, 437, 1271, 1519, 1916
BceAI ACGGC 1 cut(s) 677
BciT130I CCWGG 6 cut(s) 165, 530, 617, 622, 776, 942
BcoDI GTCTC 4 cut(s) 316, 538, 1403, 1581
BfaI CTAG 1 cut(s) 435
BfmI CTRYAG 3 cut(s) 66, 1662, 2166
BfoI RGCGCY 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 515
BglI GCCNNNNNGGC 2 cut(s) 340, 1242
BglII AGATCT 2 cut(s) 761, 1658
BisI GCNGC 6 cut(s) 14, 75, 78, 83, 348, 526
BlpI GCTNAGC 1 cut(s) 1791
BlsI GCNGC 6 cut(s) 15, 76, 79, 84, 349, 527
BmcAI AGTACT 1 cut(s) 2164
Bme1390I CCNGG 6 cut(s) 165, 530, 617, 622, 776, 942
Bme18I GGWCC 3 cut(s) 161, 1720, 1783
BmeRI GACNNNNNGTC 1 cut(s) 1594
BmeT110I CYCGRG 1 cut(s) 1908
BmgT120I GGNCC 3 cut(s) 161, 1720, 1783
BmiI GGNNCC 4 cut(s) 739, 897, 1252, 1722
BmrFI CCNGG 6 cut(s) 165, 530, 617, 622, 776, 942
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 4 cut(s) 1894, 1958, 2050, 2173
BmuI ACTGGG 1 cut(s) 469
BpiI GAAGAC 1 cut(s) 1590
Bpu1102I GCTNAGC 1 cut(s) 1791
Bpu14I TTCGAA 2 cut(s) 189, 1184
BpuEI CTTGAG 2 cut(s) 275, 796
BsaAI YACGTR 1 cut(s) 465
BsaI GGTCTC 2 cut(s) 316, 538
BsaJI CCNNGG 3 cut(s) 1237, 1246, 2112
BsaXI ACNNNNNCTCC 2 cut(s) 1311, 1341
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 357, 2041, 2118
Bse118I RCCGGY 1 cut(s) 903
Bse1I ACTGG 6 cut(s) 188, 267, 475, 567, 993, 1740
Bse3DI GCAATG 1 cut(s) 517
BseBI CCWGG 6 cut(s) 165, 530, 617, 622, 776, 942
BseDI CCNNGG 3 cut(s) 1237, 1246, 2112
BseGI GGATG 6 cut(s) 10, 685, 875, 937, 1621, 2128
BseLI CCNNNNNNNGG 4 cut(s) 307, 357, 2041, 2118
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 6 cut(s) 453, 1475, 1782, 1845, 2066, 2091
BseNI ACTGG 6 cut(s) 188, 267, 475, 567, 993, 1740
BseRI GAGGAG 1 cut(s) 1499
BseXI GCAGC 5 cut(s) 25, 86, 89, 359, 537
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 4 cut(s) 620, 992, 1236, 1245
BshNI GGYRCC 1 cut(s) 1250
BsiHKAI GWGCWC 1 cut(s) 1797
BsiHKCI CYCGRG 1 cut(s) 1908
BsiSI CCGG 1 cut(s) 904
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 4 cut(s) 307, 357, 2041, 2118
BsmAI GTCTC 4 cut(s) 316, 538, 1403, 1581
BsmBI CGTCTC 1 cut(s) 1403
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 4 cut(s) 602, 893, 959, 1272
BsnI GGCC 4 cut(s) 620, 992, 1236, 1245
Bso31I GGTCTC 2 cut(s) 316, 538
BsoBI CYCGRG 1 cut(s) 1908
Bsp119I TTCGAA 2 cut(s) 189, 1184
Bsp1286I GDGCHC 1 cut(s) 1797
Bsp143I GATC 4 cut(s) 720, 761, 1658, 2092
Bsp1720I GCTNAGC 1 cut(s) 1791
BspACI CCGC 3 cut(s) 83, 238, 978
BspANI GGCC 4 cut(s) 620, 992, 1236, 1245
BspCNI CTCAG 6 cut(s) 452, 1474, 1783, 1844, 2065, 2090
BspLI GGNNCC 4 cut(s) 739, 897, 1252, 1722
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 1 cut(s) 715
BspT104I TTCGAA 2 cut(s) 189, 1184
BspT107I GGYRCC 1 cut(s) 1250
BspTNI GGTCTC 2 cut(s) 316, 538
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 903
BsrI ACTGG 6 cut(s) 188, 267, 475, 567, 993, 1740
BssAI RCCGGY 1 cut(s) 903
BssECI CCNNGG 3 cut(s) 1237, 1246, 2112
BssMI GATC 4 cut(s) 720, 761, 1658, 2092
BssSI CACGAG 1 cut(s) 1692
BssT1I CCWWGG 2 cut(s) 1237, 1246
Bst2BI CACGAG 1 cut(s) 1692
Bst2UI CCWGG 6 cut(s) 165, 530, 617, 622, 776, 942
Bst4CI ACNGT 6 cut(s) 70, 198, 310, 400, 1026, 1255
Bst6I CTCTTC 2 cut(s) 748, 799
BstAPI GCANNNNNTGC 1 cut(s) 507
BstBAI YACGTR 1 cut(s) 465
BstBI TTCGAA 2 cut(s) 189, 1184
BstC8I GCNNGC 3 cut(s) 345, 499, 690
BstDEI CTNAG 6 cut(s) 439, 1461, 1791, 1831, 2052, 2077
BstF5I GGATG 6 cut(s) 10, 685, 875, 937, 1621, 2128
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 2 cut(s) 82, 1512
BstKTI GATC 4 cut(s) 723, 764, 1661, 2095
BstMAI GTCTC 4 cut(s) 316, 538, 1403, 1581
BstMBI GATC 4 cut(s) 720, 761, 1658, 2092
BstNI CCWGG 6 cut(s) 165, 530, 617, 622, 776, 942
BstSCI CCNGG 6 cut(s) 163, 528, 615, 620, 774, 940
BstSFI CTRYAG 3 cut(s) 66, 1662, 2166
BstV1I GCAGC 5 cut(s) 25, 86, 89, 359, 537
BstV2I GAAGAC 1 cut(s) 1590
BstX2I RGATCY 2 cut(s) 761, 1658
BstYI RGATCY 2 cut(s) 761, 1658
BsuRI GGCC 4 cut(s) 620, 992, 1236, 1245
BtsCI GGATG 6 cut(s) 10, 685, 875, 937, 1621, 2128
BtsI GCAGTG 2 cut(s) 331, 914
BtsIMutI CAGTG 3 cut(s) 331, 482, 914
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 3 cut(s) 345, 499, 690
CaiI CAGNNNCTG 2 cut(s) 507, 2194
CfoI GCGC 2 cut(s) 82, 1512
Cfr10I RCCGGY 1 cut(s) 903
Cfr13I GGNCC 3 cut(s) 161, 1720, 1783
CsiI ACCWGGT 1 cut(s) 163
Csp6I GTAC 6 cut(s) 260, 504, 1251, 1549, 1697, 2163
CviAII CATG 3 cut(s) 591, 1570, 2151
CviQI GTAC 6 cut(s) 260, 504, 1251, 1549, 1697, 2163
DdeI CTNAG 6 cut(s) 439, 1461, 1791, 1831, 2052, 2077
DpnI GATC 4 cut(s) 722, 763, 1660, 2094
DpnII GATC 4 cut(s) 720, 761, 1658, 2092
DraI TTTAAA 1 cut(s) 1105
DraIII CACNNNGTG 1 cut(s) 125
DriI GACNNNNNGTC 1 cut(s) 1594
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 2 cut(s) 748, 799
Eam1105I GACNNNNNGTC 1 cut(s) 1594
EarI CTCTTC 2 cut(s) 748, 799
Ecl136II GAGCTC 1 cut(s) 1795
Eco130I CCWWGG 2 cut(s) 1237, 1246
Eco147I AGGCCT 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 1797
Eco31I GGTCTC 2 cut(s) 316, 538
Eco47I GGWCC 3 cut(s) 161, 1720, 1783
Eco53kI GAGCTC 1 cut(s) 1795
Eco88I CYCGRG 1 cut(s) 1908
EcoICRI GAGCTC 1 cut(s) 1795
EcoRI GAATTC 3 cut(s) 430, 1227, 1436
EcoRII CCWGG 6 cut(s) 163, 528, 615, 620, 774, 940
EcoT14I CCWWGG 2 cut(s) 1237, 1246
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 1797
ErhI CCWWGG 2 cut(s) 1237, 1246
Esp3I CGTCTC 1 cut(s) 1403
FaeI CATG 3 cut(s) 594, 1573, 2154
FalI AAGNNNNNCTT 2 cut(s) 1407, 1439
FaqI GGGAC 1 cut(s) 457
FatI CATG 3 cut(s) 590, 1569, 2150
FblI GTMKAC 1 cut(s) 1161
Fnu4HI GCNGC 6 cut(s) 14, 75, 78, 83, 348, 526
FokI GGATG 6 cut(s) 17, 692, 882, 924, 1628, 2135
FriOI GRGCYC 1 cut(s) 1797
Fsp4HI GCNGC 6 cut(s) 14, 75, 78, 83, 348, 526
FspBI CTAG 1 cut(s) 435
GlaI GCGC 2 cut(s) 81, 1511
GluI GCNGC 6 cut(s) 14, 75, 78, 83, 348, 526
GsaI CCCAGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 4 cut(s) 620, 992, 1236, 1245
HapII CCGG 1 cut(s) 904
HhaI GCGC 2 cut(s) 82, 1512
Hin1II CATG 3 cut(s) 594, 1573, 2154
Hin6I GCGC 2 cut(s) 80, 1510
HinP1I GCGC 2 cut(s) 80, 1510
HincII GTYRAC 2 cut(s) 769, 1162
HindII GTYRAC 2 cut(s) 769, 1162
HinfI GANTC 5 cut(s) 206, 250, 393, 934, 2031
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 1 cut(s) 904
HphI GGTGA 2 cut(s) 1160, 1334
Hpy166II GTNNAC 6 cut(s) 636, 769, 919, 1162, 1222, 1258
Hpy188I TCNGA 8 cut(s) 249, 296, 442, 757, 1824, 1834, 2059, 2080
Hpy188III TCNNGA 8 cut(s) 232, 254, 875, 1656, 1718, 1798, 1901, 1928
Hpy8I GTNNAC 6 cut(s) 636, 769, 919, 1162, 1222, 1258
HpyAV CCTTC 7 cut(s) 552, 568, 839, 860, 1084, 1180, 1871
HpyCH4III ACNGT 6 cut(s) 70, 198, 310, 400, 1026, 1255
HpyCH4IV ACGT 1 cut(s) 464
HpyF3I CTNAG 6 cut(s) 439, 1461, 1791, 1831, 2052, 2077
HpySE526I ACGT 1 cut(s) 464
Hsp92II CATG 3 cut(s) 594, 1573, 2154
HspAI GCGC 2 cut(s) 80, 1510
KpnI GGTACC 1 cut(s) 1254
KspAI GTTAAC 1 cut(s) 769
Kzo9I GATC 4 cut(s) 720, 761, 1658, 2092
LmnI GCTCC 3 cut(s) 705, 901, 1129
Lsp1109I GCAGC 5 cut(s) 25, 86, 89, 359, 537
LweI GCATC 4 cut(s) 1894, 1958, 2050, 2173
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 1 cut(s) 435
MaeII ACGT 1 cut(s) 464
MaeIII GTNAC 1 cut(s) 170
MalI GATC 4 cut(s) 722, 763, 1660, 2094
MboI GATC 4 cut(s) 720, 761, 1658, 2092
MboII GAAGA 9 cut(s) 735, 786, 1087, 1286, 1293, 1590, 1943, 1946, 2166
MfeI CAATTG 1 cut(s) 2036
MflI RGATCY 2 cut(s) 761, 1658
MhlI GDGCHC 1 cut(s) 1797
MlsI TGGCCA 1 cut(s) 1236
MluNI TGGCCA 1 cut(s) 1236
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 7 cut(s) 678, 1107, 1173, 1297, 1309, 1847, 2004
Mox20I TGGCCA 1 cut(s) 1236
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 4 cut(s) 133, 392, 742, 1456
MscI TGGCCA 1 cut(s) 1236
MslI CAYNNNNRTG 2 cut(s) 1215, 1377
Msp20I TGGCCA 1 cut(s) 1236
MspI CCGG 1 cut(s) 904
MspR9I CCNGG 6 cut(s) 165, 530, 617, 622, 776, 942
MunI CAATTG 1 cut(s) 2036
Mva1269I GAATGC 4 cut(s) 602, 893, 959, 1272
MvaI CCWGG 6 cut(s) 165, 530, 617, 622, 776, 942
NdeII GATC 4 cut(s) 720, 761, 1658, 2092
NlaIII CATG 3 cut(s) 594, 1573, 2154
NlaIV GGNNCC 4 cut(s) 739, 897, 1252, 1722
NmeAIII GCCGAG 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 604
NspV TTCGAA 2 cut(s) 189, 1184
PaeR7I CTCGAG 1 cut(s) 1908
PceI AGGCCT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 1899
PctI GAATGC 4 cut(s) 602, 893, 959, 1272
PdmI GAANNNNTTC 4 cut(s) 133, 392, 742, 1456
PfeI GAWTC 4 cut(s) 250, 393, 934, 2031
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 6 cut(s) 15, 76, 79, 84, 349, 527
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 465
PshBI ATTAAT 2 cut(s) 57, 1341
Psp124BI GAGCTC 1 cut(s) 1797
Psp6I CCWGG 6 cut(s) 163, 528, 615, 620, 774, 940
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 6 cut(s) 163, 528, 615, 620, 774, 940
PspN4I GGNNCC 4 cut(s) 739, 897, 1252, 1722
PspPI GGNCC 3 cut(s) 161, 1720, 1783
PspXI VCTCGAGB 1 cut(s) 1908
PsrI GAACNNNNNNTAC 2 cut(s) 2155, 2187
PstNI CAGNNNCTG 2 cut(s) 507, 2194
PsuI RGATCY 2 cut(s) 761, 1658
RsaI GTAC 6 cut(s) 261, 505, 1252, 1550, 1698, 2164
RsaNI GTAC 6 cut(s) 260, 504, 1251, 1549, 1697, 2163
RseI CAYNNNNRTG 2 cut(s) 1215, 1377
SacI GAGCTC 1 cut(s) 1797
SalI GTCGAC 1 cut(s) 1160
SatI GCNGC 6 cut(s) 14, 75, 78, 83, 348, 526
Sau3AI GATC 4 cut(s) 720, 761, 1658, 2092
Sau96I GGNCC 3 cut(s) 161, 1720, 1783
ScaI AGTACT 1 cut(s) 2164
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 6 cut(s) 165, 530, 617, 622, 776, 942
SduI GDGCHC 1 cut(s) 1797
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 4 cut(s) 1894, 1958, 2050, 2173
SfcI CTRYAG 3 cut(s) 66, 1662, 2166
SfiI GGCCNNNNNGGCC 1 cut(s) 1242
Sfr274I CTCGAG 1 cut(s) 1908
SfuI TTCGAA 2 cut(s) 189, 1184
SinI GGWCC 3 cut(s) 161, 1720, 1783
SlaI CTCGAG 1 cut(s) 1908
SmiMI CAYNNNNRTG 2 cut(s) 1215, 1377
SmlI CTYRAG 3 cut(s) 254, 811, 1908
SmoI CTYRAG 3 cut(s) 254, 811, 1908
SseBI AGGCCT 1 cut(s) 620
SsiI CCGC 3 cut(s) 83, 238, 978
SspI AATATT 1 cut(s) 1730
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 1 cut(s) 1797
StuI AGGCCT 1 cut(s) 620
StyD4I CCNGG 6 cut(s) 163, 528, 615, 620, 774, 940
StyI CCWWGG 2 cut(s) 1237, 1246
TaaI ACNGT 6 cut(s) 70, 198, 310, 400, 1026, 1255
TaiI ACGT 1 cut(s) 467
TaqII GACCGA 1 cut(s) 2126
TatI WGTACW 2 cut(s) 1696, 2162
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 4 cut(s) 250, 393, 934, 2031
TscAI CASTG 3 cut(s) 331, 482, 914
TseI GCWGC 5 cut(s) 13, 74, 77, 347, 525
TspGWI ACGGA 1 cut(s) 1427
TspRI CASTG 3 cut(s) 331, 482, 914
VpaK11BI GGWCC 3 cut(s) 161, 1720, 1783
VspI ATTAAT 2 cut(s) 57, 1341
XapI RAATTY 6 cut(s) 267, 430, 1227, 1333, 1436, 1975
XhoI CTCGAG 1 cut(s) 1908
XmiI GTMKAC 1 cut(s) 1161
XmnI GAANNNNTTC 4 cut(s) 133, 392, 742, 1456
XspI CTAG 1 cut(s) 435
ZrmI AGTACT 1 cut(s) 2164
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.