Rw2G026910

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000052
Physical Location & Seq
Forward (+)
41888388 .. 41892313
3926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026910.1

Sequence Viewer

Length: 2088 bp
ATGGTTTGGGACTACCCAGGAAGAAGCCACCTGGGAAGATGCTGTTACCTTACTAGCACCAGAAATGGCTACTACAACAATTCGTTCCAGATAATCTGTGACAACTCAACCAGCCCTCCCACACCTTTCTTGACCAACACAAGTCTCGAGGTGCTGGAAATCTCTATTAAAGGCACACTCCTGGTCAGAAGCCCCATCACCTTCTCAAATTGCACTGACAAGATGAGCAACCGCCAGAACCCCAATCTGAGGAATAACCCTTTCTGGTACTCCCAGGAGAACAGGTTCACTTCAATGAGTTGTGGAGGAATTGCCCTAATGACCGAGTTTTTTAATGGGTCGACAATAACACTTGGTGGCTGCTTGTCCATTTGTGACTACTCCAGTGATAGCATATTGAATAATAGCTGCGGTGGTATCAACTGCTGCCAGACCACCCTTCCTGAATATCTAGACAATTTCAATGCTAGTTTTGGAGAAGTAATAAACGCTGATCGCCGTGCTAATGAATGCAAGTATGTATTCCTTGTAGACCAAAGTTGGTTTACTACAACAAATATATCGACCATTGGTGATATGGACTTCGTCCCTGTGACGCTGGATTGGAGCTTACGCTCCTATTTGAATATCGAGATATATGGGACGAACAATTGGACTGACAGTAACAATAGCACATATAGCAGCGATGATGGACGTCTACGCTTCTGCTCAAAAGGCTATCAAGGAAACCCTTATCTTCTTCATGGATGTCAAGATATCGATGAATGTGAGGATCGATCATGTGGTAGTCACGGGATCTGTATCAATAATCCTGGAGGCTATGATTGTGACATCCCTGATCCCCCACCGGAAAAGCCGGTAAAGCTGGGCGTTATAGTTACAAGCAGTGTTTTTGGAGTTCTACTCATACTCATTTGTGGATGGTGGTCGCACAAAGTCATAAAAAAACGGAATAACATCAAACGCAAGAAGAAGTTCTTCAAACAAAATGGTGGCTTCTTGTTGGAGCAACAATTTTCTTCAGGTGAAGTCAATGTTGAGAAAATCAAGCTGTTTAATTCCAAGGAGCTAGAGAAAGCCACAGACCATTTTAATGTGGACAGGATTCTTGGTCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGCTGGCAGATGGAAGAATTGTTGCTGTGAAGAAGTCTAAACTAGTTCATGGTGGAGAAGTTAGACAATTCATCAATGAAATTGTCATTCTTTCCCAAATTATCCACAGAAATGTGGTCAAACTTTTGGGTTGTTGCTTAGAGACAGAAGCTCCTCTTTTGGTTTATGAATTCATTGCCAACGGAACATTGTCTGAATATATTCATCAGCAGAATGAAGAGTTTCCTCTTACATGGGAAATAAGACTACGAGTTGCCATCGAAGTAGCAGGAGCTCTTTCCTATTTACATTCTGCAGCTGCTTTTCCCATTTATCATCGAGATATCAAGTCATCTAACATACTCCTGGATGATAAATACAGAGCAAAAGTAGCAGACTTTGGGACTTCTCGATCTATTTCCATTGACCAAACACACCTTACCACACTAGTATATGGAACATTTGGTTATTTGGACCCAGAGTACTTCCAATCTAGCCAATTTACAGACAAGAGTGATGTTTATAGCTTTGGTGTGGTACTTGTTGAGCTCTTAACTGGACAAAAACCAGTTTCTTTGACAAGATCCCAAGAAGGAAGAAGCCTAGCAACTTATTTCCTTCTTTCTATGGATCAAAATTGTCTATTTGATGTTCTAGATCCTCAAGTTACGAGATATGGAGGGAAGGAAGAGATTACTGCAGTTGCTAACATTGCAAAAAGATGCTTAAATTTGAATGGAAAGAAACGACCTACTATGAAAGAAGTAGCAGTTGAATTGGAGGCAATTCAGTCGTCAATTAAGGCCTCTAATCATGTTGATCAAAATTTGGGAGAGGTTGCATATATCCAAACTCATGAAATAACCGAGGCTTGGGATGTTGTTTCTACATCAACAGGGCCTTGTGTCGATGGTGGTAGTTCTTCGTTCGATGCACAACCACTGTTGACTATGAAGACTATGTGA

Protein Analysis

695

Amino Acids

77.61

Weight (kDa)

5.72

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 364 - 630 2.4e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 366 - 633 8.9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 697
AccI GTMKAC 3 cut(s) 341, 531, 697
AciI CCGC 2 cut(s) 232, 411
AclWI GGATC 6 cut(s) 780, 803, 833, 1701, 1761, 1775
AcsI RAATTY 3 cut(s) 1313, 1852, 1948
AcuI CTGAAG 1 cut(s) 1005
AcyI GRCGYC 1 cut(s) 694
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 4 cut(s) 269, 1129, 1607, 1662
AfiI CCNNNNNNNGG 2 cut(s) 249, 1995
AgsI TTSAA 7 cut(s) 294, 400, 463, 625, 982, 1858, 1898
AhlI ACTAGT 2 cut(s) 1186, 1570
AjnI CCWGG 6 cut(s) 16, 30, 180, 273, 811, 1488
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
Alw21I GWGCWC 2 cut(s) 1420, 1674
Alw26I GTCTC 2 cut(s) 149, 1280
AlwI GGATC 6 cut(s) 780, 803, 833, 1701, 1761, 1775
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 3 cut(s) 1120, 1926, 2021
ApeKI GCWGC 6 cut(s) 360, 408, 426, 681, 1439, 1442
ApoI RAATTY 3 cut(s) 1313, 1852, 1948
Asp700I GAANNNNTTC 5 cut(s) 284, 974, 977, 1344, 1365
AspS9I GGNCC 2 cut(s) 1597, 2021
AsuHPI GGTGA 3 cut(s) 190, 584, 1037
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 1 cut(s) 1597
BanII GRGCYC 2 cut(s) 1420, 1674
BbsI GAAGAC 1 cut(s) 2084
Bbv12I GWGCWC 2 cut(s) 1420, 1674
BbvI GCAGC 6 cut(s) 347, 395, 413, 693, 1429, 1451
BccI CCATC 6 cut(s) 203, 683, 915, 1148, 1409, 2027
BceAI ACGGC 1 cut(s) 483
BcgI CGANNNNNNTGC 2 cut(s) 1896, 1930
BciT130I CCWGG 6 cut(s) 18, 32, 182, 275, 813, 1490
BclI TGATCA 1 cut(s) 1942
BcoDI GTCTC 2 cut(s) 149, 1280
BcuI ACTAGT 2 cut(s) 1186, 1570
BfaI CTAG 9 cut(s) 54, 452, 468, 1070, 1187, 1571, 1617, 1727, 1778
BfmI CTRYAG 2 cut(s) 1437, 1821
BisI GCNGC 6 cut(s) 361, 409, 427, 682, 1440, 1443
BlsI GCNGC 6 cut(s) 362, 410, 428, 683, 1441, 1444
BmcAI AGTACT 1 cut(s) 1607
Bme1390I CCNGG 6 cut(s) 18, 32, 182, 275, 813, 1490
Bme18I GGWCC 1 cut(s) 1597
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 1597, 2021
BmiI GGNNCC 1 cut(s) 1599
BmrFI CCNGG 6 cut(s) 18, 32, 182, 275, 813, 1490
BmsI GCATC 3 cut(s) 29, 1835, 2044
BpiI GAAGAC 1 cut(s) 2084
BplI GAGNNNNNCTC 2 cut(s) 888, 920
BpmI CTGGAG 2 cut(s) 367, 834
BpuEI CTTGAG 1 cut(s) 1770
Bsa29I ATCGAT 2 cut(s) 759, 775
BsaBI GATNNNNATC 1 cut(s) 800
BsaHI GRCGYC 1 cut(s) 694
BsaJI CCNNGG 6 cut(s) 16, 31, 273, 1062, 1123, 1989
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 269, 299, 1279, 1309
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 1995
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 3 cut(s) 384, 1684, 1691
Bse3DI GCAATG 2 cut(s) 1317, 1833
Bse8I GATNNNNATC 1 cut(s) 800
BseBI CCWGG 6 cut(s) 18, 32, 182, 275, 813, 1490
BseCI ATCGAT 2 cut(s) 759, 775
BseDI CCNNGG 6 cut(s) 16, 31, 273, 1062, 1123, 1989
BseGI GGATG 5 cut(s) 752, 831, 926, 1498, 2005
BseJI GATNNNNATC 1 cut(s) 800
BseLI CCNNNNNNNGG 2 cut(s) 249, 1995
BseMI GCAATG 2 cut(s) 1317, 1833
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 3 cut(s) 384, 1684, 1691
BseRI GAGGAG 1 cut(s) 1287
BseXI GCAGC 6 cut(s) 347, 395, 413, 693, 1429, 1451
BseYI CCCAGC 1 cut(s) 865
BshFI GGCC 3 cut(s) 1122, 1928, 2023
BshVI ATCGAT 2 cut(s) 759, 775
BsiHKAI GWGCWC 2 cut(s) 1420, 1674
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 2 cut(s) 848, 857
BslFI GGGAC 4 cut(s) 23, 572, 655, 1540
BslI CCNNNNNNNGG 2 cut(s) 249, 1995
BsmAI GTCTC 2 cut(s) 149, 1280
BsmFI GGGAC 4 cut(s) 23, 572, 655, 1540
BsmI GAATGC 2 cut(s) 515, 1149
BsnI GGCC 3 cut(s) 1122, 1928, 2023
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 1420, 1674
BspACI CCGC 2 cut(s) 232, 411
BspANI GGCC 3 cut(s) 1122, 1928, 2023
BspCNI CTCAG 1 cut(s) 240
BspDI ATCGAT 2 cut(s) 759, 775
BspHI TCATGA 1 cut(s) 1978
BspLI GGNNCC 1 cut(s) 1599
BspMAI CTGCAG 2 cut(s) 1441, 1825
BspPI GGATC 6 cut(s) 780, 803, 833, 1701, 1761, 1775
BsrDI GCAATG 2 cut(s) 1317, 1833
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 3 cut(s) 384, 1684, 1691
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 6 cut(s) 16, 31, 273, 1062, 1123, 1989
BssNI GRCGYC 1 cut(s) 694
BssT1I CCWWGG 2 cut(s) 1062, 1123
Bst2UI CCWGG 6 cut(s) 18, 32, 182, 275, 813, 1490
Bst4CI ACNGT 3 cut(s) 662, 1132, 2067
Bst6I CTCTTC 2 cut(s) 1356, 1806
BstACI GRCGYC 1 cut(s) 694
BstC8I GCNNGC 1 cut(s) 1149
BstDEI CTNAG 2 cut(s) 248, 1282
BstF5I GGATG 5 cut(s) 752, 831, 926, 1498, 2005
BstMAI GTCTC 2 cut(s) 149, 1280
BstMWI GCNNNNNNNGC 5 cut(s) 678, 714, 862, 1514, 1835
BstNI CCWGG 6 cut(s) 18, 32, 182, 275, 813, 1490
BstSCI CCNGG 6 cut(s) 16, 30, 180, 273, 811, 1488
BstSFI CTRYAG 2 cut(s) 1437, 1821
BstV1I GCAGC 6 cut(s) 347, 395, 413, 693, 1429, 1451
BstV2I GAAGAC 1 cut(s) 2084
BstX2I RGATCY 3 cut(s) 795, 1706, 1780
BstYI RGATCY 3 cut(s) 795, 1706, 1780
Bsu15I ATCGAT 2 cut(s) 759, 775
BsuRI GGCC 3 cut(s) 1122, 1928, 2023
BsuTUI ATCGAT 2 cut(s) 759, 775
BtgZI GCGATG 1 cut(s) 699
BtsCI GGATG 5 cut(s) 752, 831, 926, 1498, 2005
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 4 cut(s) 213, 391, 892, 2063
Cac8I GCNNGC 1 cut(s) 1149
CciI TCATGA 1 cut(s) 1978
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 2 cut(s) 1597, 2021
ClaI ATCGAT 2 cut(s) 759, 775
CseI GACGC 1 cut(s) 604
Csp6I GTAC 4 cut(s) 268, 1128, 1606, 1661
CviAII CATG 6 cut(s) 743, 780, 1193, 1377, 1937, 1979
CviQI GTAC 4 cut(s) 268, 1128, 1606, 1661
DdeI CTNAG 2 cut(s) 248, 1282
DraIII CACNNNGTG 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 1356, 1806
EarI CTCTTC 2 cut(s) 1356, 1806
Ecl136II GAGCTC 2 cut(s) 1418, 1672
Eco130I CCWWGG 2 cut(s) 1062, 1123
Eco147I AGGCCT 1 cut(s) 1928
Eco24I GRGCYC 2 cut(s) 1420, 1674
Eco32I GATATC 2 cut(s) 757, 1468
Eco47I GGWCC 1 cut(s) 1597
Eco53kI GAGCTC 2 cut(s) 1418, 1672
Eco57I CTGAAG 1 cut(s) 1005
Eco88I CYCGRG 1 cut(s) 146
EcoICRI GAGCTC 2 cut(s) 1418, 1672
EcoO109I RGGNCCY 1 cut(s) 2021
EcoRI GAATTC 1 cut(s) 1313
EcoRII CCWGG 6 cut(s) 16, 30, 180, 273, 811, 1488
EcoRV GATATC 2 cut(s) 757, 1468
EcoT14I CCWWGG 2 cut(s) 1062, 1123
EcoT38I GRGCYC 2 cut(s) 1420, 1674
ErhI CCWWGG 2 cut(s) 1062, 1123
FaeI CATG 6 cut(s) 746, 783, 1196, 1380, 1940, 1982
FalI AAGNNNNNCTT 4 cut(s) 962, 994, 1284, 1316
FaqI GGGAC 4 cut(s) 23, 572, 655, 1540
FatI CATG 6 cut(s) 742, 779, 1192, 1376, 1936, 1978
FbaI TGATCA 1 cut(s) 1942
FblI GTMKAC 3 cut(s) 341, 531, 697
Fnu4HI GCNGC 6 cut(s) 361, 409, 427, 682, 1440, 1443
FokI GGATG 5 cut(s) 759, 818, 933, 1505, 2012
FriOI GRGCYC 2 cut(s) 1420, 1674
Fsp4HI GCNGC 6 cut(s) 361, 409, 427, 682, 1440, 1443
FspBI CTAG 9 cut(s) 54, 452, 468, 1070, 1187, 1571, 1617, 1727, 1778
GluI GCNGC 6 cut(s) 361, 409, 427, 682, 1440, 1443
GsaI CCCAGC 1 cut(s) 869
GsuI CTGGAG 2 cut(s) 367, 834
HaeIII GGCC 3 cut(s) 1122, 1928, 2023
HapII CCGG 2 cut(s) 848, 857
HgaI GACGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 694
Hin1II CATG 6 cut(s) 746, 783, 1196, 1380, 1940, 1982
HincII GTYRAC 2 cut(s) 342, 2070
HindII GTYRAC 2 cut(s) 342, 2070
HinfI GANTC 1 cut(s) 1105
HpaII CCGG 2 cut(s) 848, 857
HphI GGTGA 3 cut(s) 190, 584, 1037
Hpy166II GTNNAC 8 cut(s) 288, 342, 532, 546, 698, 1099, 1135, 2070
Hpy188I TCNGA 3 cut(s) 188, 249, 1339
Hpy8I GTNNAC 8 cut(s) 288, 342, 532, 546, 698, 1099, 1135, 2070
HpyAV CCTTC 5 cut(s) 211, 449, 1709, 1751, 1801
HpyCH4III ACNGT 3 cut(s) 662, 1132, 2067
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 7 cut(s) 213, 513, 1439, 1823, 1838, 1964, 2057
HpyF10VI GCNNNNNNNGC 5 cut(s) 678, 714, 862, 1514, 1835
HpyF3I CTNAG 2 cut(s) 248, 1282
HpySE526I ACGT 1 cut(s) 694
Hsp92I GRCGYC 1 cut(s) 694
Hsp92II CATG 6 cut(s) 746, 783, 1196, 1380, 1940, 1982
Ksp22I TGATCA 1 cut(s) 1942
LmnI GCTCC 6 cut(s) 606, 620, 1006, 1066, 1300, 1415
Lsp1109I GCAGC 6 cut(s) 347, 395, 413, 693, 1429, 1451
LweI GCATC 3 cut(s) 29, 1835, 2044
MaeI CTAG 9 cut(s) 54, 452, 468, 1070, 1187, 1571, 1617, 1727, 1778
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 9 cut(s) 44, 98, 374, 592, 662, 788, 827, 877, 1789
MfeI CAATTG 1 cut(s) 649
MflI RGATCY 3 cut(s) 795, 1706, 1780
MhlI GDGCHC 2 cut(s) 1420, 1674
MmeI TCCRAC 1 cut(s) 984
MroXI GAANNNNTTC 5 cut(s) 284, 974, 977, 1344, 1365
MseI TTAA 7 cut(s) 168, 333, 1056, 1092, 1676, 1850, 1923
MslI CAYNNNNRTG 3 cut(s) 293, 1092, 1254
MspA1I CMGCKG 1 cut(s) 1442
MspI CCGG 2 cut(s) 848, 857
MspR9I CCNGG 6 cut(s) 18, 32, 182, 275, 813, 1490
MunI CAATTG 1 cut(s) 649
Mva1269I GAATGC 2 cut(s) 515, 1149
MvaI CCWGG 6 cut(s) 18, 32, 182, 275, 813, 1490
MwoI GCNNNNNNNGC 5 cut(s) 678, 714, 862, 1514, 1835
NlaIII CATG 6 cut(s) 746, 783, 1196, 1380, 1940, 1982
NlaIV GGNNCC 1 cut(s) 1599
NmuCI GTSAC 5 cut(s) 98, 374, 592, 788, 827
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 1 cut(s) 1978
PceI AGGCCT 1 cut(s) 1928
PctI GAATGC 2 cut(s) 515, 1149
PdmI GAANNNNTTC 5 cut(s) 284, 974, 977, 1344, 1365
PfeI GAWTC 1 cut(s) 1105
PflFI GACNNNGTC 1 cut(s) 584
PfoI TCCNGGA 2 cut(s) 811, 1488
PkrI GCNGC 6 cut(s) 362, 410, 428, 683, 1441, 1444
Psp124BI GAGCTC 2 cut(s) 1420, 1674
Psp6I CCWGG 6 cut(s) 16, 30, 180, 273, 811, 1488
PspFI CCCAGC 1 cut(s) 865
PspGI CCWGG 6 cut(s) 16, 30, 180, 273, 811, 1488
PspN4I GGNNCC 1 cut(s) 1599
PspPI GGNCC 2 cut(s) 1597, 2021
PstI CTGCAG 2 cut(s) 1441, 1825
PsuI RGATCY 3 cut(s) 795, 1706, 1780
PsyI GACNNNGTC 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 1442
RsaI GTAC 4 cut(s) 269, 1129, 1607, 1662
RsaNI GTAC 4 cut(s) 268, 1128, 1606, 1661
RseI CAYNNNNRTG 3 cut(s) 293, 1092, 1254
SacI GAGCTC 2 cut(s) 1420, 1674
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 7 cut(s) 168, 333, 1056, 1092, 1676, 1850, 1923
SatI GCNGC 6 cut(s) 361, 409, 427, 682, 1440, 1443
Sau96I GGNCC 2 cut(s) 1597, 2021
ScaI AGTACT 1 cut(s) 1607
ScrFI CCNGG 6 cut(s) 18, 32, 182, 275, 813, 1490
SduI GDGCHC 2 cut(s) 1420, 1674
SfaNI GCATC 3 cut(s) 29, 1835, 2044
SfcI CTRYAG 2 cut(s) 1437, 1821
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 1 cut(s) 1597
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 3 cut(s) 293, 1092, 1254
SmlI CTYRAG 2 cut(s) 146, 1785
SmoI CTYRAG 2 cut(s) 146, 1785
SpeI ACTAGT 2 cut(s) 1186, 1570
SseBI AGGCCT 1 cut(s) 1928
SsiI CCGC 2 cut(s) 232, 411
SspMI CTAG 9 cut(s) 54, 452, 468, 1070, 1187, 1571, 1617, 1727, 1778
SstI GAGCTC 2 cut(s) 1420, 1674
StuI AGGCCT 1 cut(s) 1928
StyD4I CCNGG 6 cut(s) 16, 30, 180, 273, 811, 1488
StyI CCWWGG 2 cut(s) 1062, 1123
TaaI ACNGT 3 cut(s) 662, 1132, 2067
TaiI ACGT 1 cut(s) 697
TaqII GACCGA 1 cut(s) 338
TatI WGTACW 1 cut(s) 1605
TfiI GAWTC 1 cut(s) 1105
Tru1I TTAA 7 cut(s) 168, 333, 1056, 1092, 1676, 1850, 1923
Tru9I TTAA 7 cut(s) 168, 333, 1056, 1092, 1676, 1850, 1923
TscAI CASTG 4 cut(s) 220, 391, 892, 2070
TseFI GTSAC 5 cut(s) 98, 374, 592, 788, 827
TseI GCWGC 6 cut(s) 360, 408, 426, 681, 1439, 1442
Tsp45I GTSAC 5 cut(s) 98, 374, 592, 788, 827
TspGWI ACGGA 2 cut(s) 964, 1341
TspRI CASTG 4 cut(s) 220, 391, 892, 2070
Tth111I GACNNNGTC 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 1597
XapI RAATTY 3 cut(s) 1313, 1852, 1948
XbaI TCTAGA 2 cut(s) 451, 1777
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 3 cut(s) 341, 531, 697
XmnI GAANNNNTTC 5 cut(s) 284, 974, 977, 1344, 1365
XspI CTAG 9 cut(s) 54, 452, 468, 1070, 1187, 1571, 1617, 1727, 1778
ZraI GACGTC 1 cut(s) 695
ZrmI AGTACT 1 cut(s) 1607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.