MD09G1145500.v1.1

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000052
Physical Location & Seq
Forward (+)
11315848 .. 11319739
3892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1145500.v1.1.491

Sequence Viewer

Length: 2214 bp
ATGGTTGTCAAGTTGCTTAGTCATGTTATTCTGTTTACGCTTTTGCTGCGGTTACTAGCAGTAGCATCATCACAGAAACCGCCCATAGCAAAGCCTAACTGTCAGTCTCATTGTGGAGACATTGAAATCCCGTATCCCTTTGGAATTGGGGCAGGTTGTTACATTGACGACGACTGGTTCCAAATAATCTGTGACAACTCTACTGGCAATCCCACTCCTTTCTTGAACCGTACTAATCTGGAGGTCCTCGAAATTTCTGCTGAAGGAGGTACGCTAAAGGTCACAAACCCGATCACCTTCTCAAATTGCAGCAACAAGCCAATCAACCGCCAGACGGCCAACTTGGAGGGAAGCCCTTTTGCGTATTCCACCAAGAACGTGTTCACTGCAGTTGGTTGTGGTTTTATGGCCACGATCACCTCAAACTCAAATGGCTACTCCATTTCAAGTGGATGCAAGTCCGAGTGTGACATTTCCATAAATAAGAGTAACGGTACCAGGCATAATGTATGCAACGGTGTGGACTGCTGCCAGACAGCCATCCCTTCATTTCTCTCTGCCTTCAATACTAGTTTCCAGCGTGATGACGATAAAACACGAAGTTCATGCAATTACGCATTCTTGGTAGACCGCGAATGGTTTCAGGGGTATGCCAACAATTTAACAAACATTTCTGCCATCAGCGACAGGGACAATATTCCTGTGGTGCTTGAATGGAACATGTTTCACTCCACAACTGAAGTGTTTGGGACCTTTTTGGTAGTAAATGCAACGGTATGGAGGGATGACCAATCCGGCCTTCATGTGATAGCTACAATGACAACTCTTCCGTGTATGGATCCTCAAGGCTTGAATGTACCTGCGGAGGGGGTTCGGAAGGAAATCCCTATCTTCTCGACGGATGTCAAGGTTGGCAAGCATTCGTGTTCCGAACCCCTAGTATGTGTGAATCATCGTTCGGGTTACGGATGCTATTATCCTCCGAAGAGCAAATCTCCAGTCAAAGCCATCATTATAGGTATCGGCAGTGGTCTTGGATTCTTGTTACTGCTCCTTGGTGCCTGGTGGTTGCATAAACTCATAAAGAAAACAAAAGCCATTAAACGCAAGAAGAAGTTCTTTAAACAAAATGGTGGTATACTATTGGAGCAACAACTGGCGGCTGGTGAAGTAAATGTTGAGAAAATTAAGTTGTTCAATTCACAGGAGTTAGAGATGGCCACAGACCATTTTAACATCGATAGAATTCTTGGCCAGGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGACGGATGGAAGAATCGTTGCTGTAAAGAAGTCTAAATTAGTTGATGGAGGCGAAATTGCGCAGTTCATTAACGAAATTGTCATTCTTTCGCAAATTAACCATAGGAATGTGGTTAAACTGTTGGGTTGCTGTTTAGAGACAGAAGTACCTCTCTTGGTTTATGAATTCATACCGAAAGGGACTATCTACCAATATCTCCACAAAGAAAATGAGGAGTTTTTATTTACGTGGGAAATGCGCTTAAGGATTGCTGCTGAAGTTGCAGGAGCTCTTTCATACTTACATTCAGCAGCAGGTTTTCCAATTTACCACAGGGACATCAAGTCTACAAACATACTCTTAGATGATAAATACAGAGCAAAAGTTGCAGATTTCGGGACTTCAAGATCGGTTTCCATTGACCAAACCCACCTCACCACAATAGTACATGGCACCTTCGGGTACTTGGACCCAGAGTACTTCCAATCAAGTCAGTTTACGGATAAGAGTGATGTGTATAGCTTTGGAGTTGTCCTTCTTGAGCTGTTGACTGGAGAAAAATCCGTTTCTCTAACAAGGTCACCAGAAATCAGAGGCCTAGTTACATATTTTAATTTTTCAATGGAAGAGAATCGTCTGTTTGATATTATTGATGCTCGAGTTAAGGAGGAGGGTGTAAAAGAAGACATATTGTCAGTTGCTAACCTCGCAAACAGATGCTTGGATATGAGTGGAAAGAGACGGCCTACGATGAAAGAAGTGGCAATGGAGTTGGAGAGGATTCAAAAATCAGTCAACGCTTCTAATAATCTGCAACAAAATTCCGATGAGGTCGAATATGTTAGAAATGAAGTAACTGGTCCTTGGGATGTTACATCGACAGGGTCGTGTTTGGCTGGAGGTACTACTCCGTCAAAAGATTCACTACCACTACTATCTTACTGA

Protein Analysis

738

Amino Acids

81.65

Weight (kDa)

6.34

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 95 3.3e-12 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 412 - 681 2.9e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 412 - 677 1.7e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1350
Acc36I ACCTGC 3 cut(s) 143, 868, 1574
Acc65I GGTACC 1 cut(s) 494
AccB1I GGYRCC 3 cut(s) 494, 1058, 1721
AccI GTMKAC 3 cut(s) 627, 1138, 1616
AccII CGCG 1 cut(s) 633
AciI CCGC 6 cut(s) 49, 80, 328, 631, 863, 1160
AclWI GGATC 2 cut(s) 833, 846
AcoI YGGCCR 4 cut(s) 336, 408, 1218, 1252
AcsI RAATTY 4 cut(s) 252, 1245, 1454, 2089
AcuI CTGAAG 3 cut(s) 282, 759, 1566
AfiI CCNNNNNNNGG 2 cut(s) 334, 866
AflII CTTAAG 1 cut(s) 1531
AflIII ACRYGT 2 cut(s) 378, 720
AhdI GACNNNNNGTC 2 cut(s) 1612, 1960
AhlI ACTAGT 1 cut(s) 569
AjnI CCWGG 3 cut(s) 497, 1061, 1254
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 783, 815
AluBI AGCT 4 cut(s) 812, 1559, 1791, 1813
AluI AGCT 4 cut(s) 812, 1559, 1791, 1813
Alw21I GWGCWC 1 cut(s) 1561
Alw26I GTCTC 4 cut(s) 111, 111, 1421, 2002
AlwI GGATC 2 cut(s) 833, 846
Ama87I CYCGRG 1 cut(s) 1926
AoxI GGCC 8 cut(s) 336, 408, 796, 1218, 1252, 1261, 1864, 2012
ApeKI GCWGC 5 cut(s) 46, 309, 528, 1541, 1580
ApoI RAATTY 4 cut(s) 252, 1245, 1454, 2089
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
Asp700I GAANNNNTTC 3 cut(s) 380, 639, 1115
Asp718I GGTACC 1 cut(s) 494
AspLEI GCGC 2 cut(s) 1351, 1530
AspS9I GGNCC 4 cut(s) 244, 750, 1738, 2129
AsuHPI GGTGA 5 cut(s) 286, 409, 1178, 1696, 1842
AvaI CYCGRG 1 cut(s) 1926
AvaII GGWCC 4 cut(s) 244, 750, 1738, 2129
BaeI ACNNNNGTAYC 2 cut(s) 1419, 1452
BalI TGGCCA 3 cut(s) 410, 1220, 1254
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 3 cut(s) 494, 1058, 1721
BanII GRGCYC 1 cut(s) 1561
BbsI GAAGAC 1 cut(s) 1959
Bbv12I GWGCWC 1 cut(s) 1561
BbvI GCAGC 5 cut(s) 33, 321, 515, 1528, 1592
BccI CCATC 6 cut(s) 548, 686, 1016, 1210, 1289, 1328
BceAI ACGGC 2 cut(s) 351, 2027
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 588, 622
BciT130I CCWGG 3 cut(s) 499, 1063, 1256
BciVI GTATCC 1 cut(s) 144
BcoDI GTCTC 4 cut(s) 111, 111, 1421, 2002
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 4 cut(s) 56, 570, 938, 1868
BfmI CTRYAG 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 1531
BfuAI ACCTGC 3 cut(s) 143, 868, 1574
BfuI GTATCC 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 1260
BisI GCNGC 6 cut(s) 47, 310, 529, 1161, 1542, 1581
BlsI GCNGC 6 cut(s) 48, 311, 530, 1162, 1543, 1582
BmcAI AGTACT 1 cut(s) 1748
Bme1390I CCNGG 3 cut(s) 499, 1063, 1256
Bme18I GGWCC 4 cut(s) 244, 750, 1738, 2129
BmeRI GACNNNNNGTC 2 cut(s) 1612, 1960
BmeT110I CYCGRG 1 cut(s) 1926
BmgT120I GGNCC 4 cut(s) 244, 750, 1738, 2129
BmiI GGNNCC 7 cut(s) 179, 496, 751, 840, 1060, 1723, 1740
BmrFI CCNGG 3 cut(s) 499, 1063, 1256
BmsI GCATC 5 cut(s) 74, 443, 959, 1912, 1976
BoxI GACNNNNGTC 1 cut(s) 902
BpiI GAAGAC 1 cut(s) 1959
BplI GAGNNNNNCTC 2 cut(s) 979, 1011
BpmI CTGGAG 4 cut(s) 260, 981, 1842, 2187
BpuEI CTTGAG 2 cut(s) 828, 1829
Bsa29I ATCGAT 1 cut(s) 1239
BsaAI YACGTR 1 cut(s) 1518
BsaJI CCNNGG 4 cut(s) 1054, 1255, 1264, 2132
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 866
Bse1I ACTGG 6 cut(s) 179, 208, 998, 1161, 1825, 2131
Bse3DI GCAATG 1 cut(s) 2040
BseBI CCWGG 3 cut(s) 499, 1063, 1256
BseCI ATCGAT 1 cut(s) 1239
BseDI CCNNGG 4 cut(s) 1054, 1255, 1264, 2132
BseGI GGATG 7 cut(s) 458, 540, 790, 907, 974, 1300, 2143
BseLI CCNNNNNNNGG 2 cut(s) 334, 866
BseMI GCAATG 1 cut(s) 2040
BseNI ACTGG 6 cut(s) 179, 208, 998, 1161, 1825, 2131
BseRI GAGGAG 2 cut(s) 1517, 1952
BseXI GCAGC 5 cut(s) 33, 321, 515, 1528, 1592
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 8 cut(s) 338, 410, 798, 1220, 1254, 1263, 1866, 2014
BshNI GGYRCC 3 cut(s) 494, 1058, 1721
BshVI ATCGAT 1 cut(s) 1239
BsiHKAI GWGCWC 1 cut(s) 1561
BsiHKCI CYCGRG 1 cut(s) 1926
BsiSI CCGG 1 cut(s) 795
BslFI GGGAC 5 cut(s) 704, 763, 1483, 1619, 1681
BslI CCNNNNNNNGG 2 cut(s) 334, 866
BsmAI GTCTC 4 cut(s) 111, 111, 1421, 2002
BsmBI CGTCTC 1 cut(s) 2002
BsmFI GGGAC 5 cut(s) 704, 763, 1483, 1619, 1681
BsmI GAATGC 2 cut(s) 617, 919
BsnI GGCC 8 cut(s) 338, 410, 798, 1220, 1254, 1263, 1866, 2014
BsoBI CYCGRG 1 cut(s) 1926
Bsp1286I GDGCHC 1 cut(s) 1561
Bsp143I GATC 4 cut(s) 291, 414, 838, 1676
BspACI CCGC 6 cut(s) 49, 80, 328, 631, 863, 1160
BspANI GGCC 8 cut(s) 338, 410, 798, 1220, 1254, 1263, 1866, 2014
BspDI ATCGAT 1 cut(s) 1239
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 7 cut(s) 179, 496, 751, 840, 1060, 1723, 1740
BspMAI CTGCAG 1 cut(s) 391
BspMI ACCTGC 3 cut(s) 143, 868, 1574
BspPI GGATC 2 cut(s) 833, 846
BspQI GCTCTTC 1 cut(s) 980
BspT107I GGYRCC 3 cut(s) 494, 1058, 1721
BspTI CTTAAG 1 cut(s) 1531
BsrDI GCAATG 1 cut(s) 2040
BsrI ACTGG 6 cut(s) 179, 208, 998, 1161, 1825, 2131
BssECI CCNNGG 4 cut(s) 1054, 1255, 1264, 2132
BssMI GATC 4 cut(s) 291, 414, 838, 1676
BssNAI GTATAC 1 cut(s) 1139
BssT1I CCWWGG 3 cut(s) 1054, 1264, 2132
Bst1107I GTATAC 1 cut(s) 1139
Bst2UI CCWGG 3 cut(s) 499, 1063, 1256
Bst4CI ACNGT 7 cut(s) 101, 230, 494, 518, 775, 1273, 1410
Bst6I CTCTTC 3 cut(s) 831, 980, 1890
BstAFI CTTAAG 1 cut(s) 1531
BstAPI GCANNNNNTGC 1 cut(s) 1655
BstBAI YACGTR 1 cut(s) 1518
BstC8I GCNNGC 1 cut(s) 917
BstDEI CTNAG 2 cut(s) 17, 1630
BstEII GGTNACC 1 cut(s) 1848
BstF5I GGATG 7 cut(s) 458, 540, 790, 907, 974, 1300, 2143
BstFNI CGCG 1 cut(s) 633
BstHHI GCGC 2 cut(s) 1351, 1530
BstKTI GATC 4 cut(s) 294, 417, 841, 1679
BstMAI GTCTC 4 cut(s) 111, 111, 1421, 2002
BstMBI GATC 4 cut(s) 291, 414, 838, 1676
BstMWI GCNNNNNNNGC 5 cut(s) 46, 1260, 1550, 1655, 1976
BstNI CCWGG 3 cut(s) 499, 1063, 1256
BstNSI RCATGY 1 cut(s) 724
BstPAI GACNNNNGTC 1 cut(s) 902
BstPI GGTNACC 1 cut(s) 1848
BstSCI CCNGG 3 cut(s) 497, 1061, 1254
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 5 cut(s) 33, 321, 515, 1528, 1592
BstV2I GAAGAC 1 cut(s) 1959
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BstZ17I GTATAC 1 cut(s) 1139
Bsu15I ATCGAT 1 cut(s) 1239
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 8 cut(s) 338, 410, 798, 1220, 1254, 1263, 1866, 2014
BsuTUI ATCGAT 1 cut(s) 1239
BtsCI GGATG 7 cut(s) 458, 540, 790, 907, 974, 1300, 2143
BtsI GCAGTG 2 cut(s) 384, 1033
BtsIMutI CAGTG 2 cut(s) 384, 1033
BveI ACCTGC 3 cut(s) 143, 868, 1574
Cac8I GCNNGC 1 cut(s) 917
CfoI GCGC 2 cut(s) 1351, 1530
Cfr13I GGNCC 4 cut(s) 244, 750, 1738, 2129
ClaI ATCGAT 1 cut(s) 1239
CviAII CATG 5 cut(s) 23, 606, 721, 803, 1718
DdeI CTNAG 2 cut(s) 17, 1630
DpnI GATC 4 cut(s) 293, 416, 840, 1678
DpnII GATC 4 cut(s) 291, 414, 838, 1676
DraI TTTAAA 1 cut(s) 1123
DriI GACNNNNNGTC 2 cut(s) 1612, 1960
EaeI YGGCCR 4 cut(s) 336, 408, 1218, 1252
Eam1104I CTCTTC 3 cut(s) 831, 980, 1890
Eam1105I GACNNNNNGTC 2 cut(s) 1612, 1960
EarI CTCTTC 3 cut(s) 831, 980, 1890
Ecl136II GAGCTC 1 cut(s) 1559
Eco130I CCWWGG 3 cut(s) 1054, 1264, 2132
Eco147I AGGCCT 1 cut(s) 1866
Eco24I GRGCYC 1 cut(s) 1561
Eco47I GGWCC 4 cut(s) 244, 750, 1738, 2129
Eco53kI GAGCTC 1 cut(s) 1559
Eco57I CTGAAG 3 cut(s) 282, 759, 1566
Eco88I CYCGRG 1 cut(s) 1926
Eco91I GGTNACC 1 cut(s) 1848
EcoICRI GAGCTC 1 cut(s) 1559
EcoO109I RGGNCCY 2 cut(s) 244, 750
EcoO65I GGTNACC 1 cut(s) 1848
EcoRI GAATTC 2 cut(s) 1245, 1454
EcoRII CCWGG 3 cut(s) 497, 1061, 1254
EcoT14I CCWWGG 3 cut(s) 1054, 1264, 2132
EcoT38I GRGCYC 1 cut(s) 1561
ErhI CCWWGG 3 cut(s) 1054, 1264, 2132
Esp3I CGTCTC 1 cut(s) 2002
FaeI CATG 5 cut(s) 26, 609, 724, 806, 1721
FalI AAGNNNNNCTT 2 cut(s) 1103, 1135
FaqI GGGAC 5 cut(s) 704, 763, 1483, 1619, 1681
FatI CATG 5 cut(s) 22, 605, 720, 802, 1717
FblI GTMKAC 3 cut(s) 627, 1138, 1616
Fnu4HI GCNGC 6 cut(s) 47, 310, 529, 1161, 1542, 1581
FokI GGATG 7 cut(s) 465, 527, 797, 914, 981, 1307, 2150
FriOI GRGCYC 1 cut(s) 1561
Fsp4HI GCNGC 6 cut(s) 47, 310, 529, 1161, 1542, 1581
FspBI CTAG 4 cut(s) 56, 570, 938, 1868
FspI TGCGCA 1 cut(s) 1350
GlaI GCGC 2 cut(s) 1350, 1529
GluI GCNGC 6 cut(s) 47, 310, 529, 1161, 1542, 1581
GsuI CTGGAG 4 cut(s) 260, 981, 1842, 2187
HaeIII GGCC 8 cut(s) 338, 410, 798, 1220, 1254, 1263, 1866, 2014
HapII CCGG 1 cut(s) 795
HhaI GCGC 2 cut(s) 1351, 1530
Hin1II CATG 5 cut(s) 26, 609, 724, 806, 1721
Hin6I GCGC 2 cut(s) 1349, 1528
HinP1I GCGC 2 cut(s) 1349, 1528
HincII GTYRAC 3 cut(s) 1290, 1818, 2065
HindII GTYRAC 3 cut(s) 1290, 1818, 2065
HinfI GANTC 6 cut(s) 949, 1038, 1302, 1900, 2050, 2189
HpaII CCGG 1 cut(s) 795
HphI GGTGA 5 cut(s) 286, 409, 1178, 1696, 1842
Hpy188I TCNGA 6 cut(s) 463, 876, 931, 984, 1862, 2095
Hpy188III TCNNGA 6 cut(s) 223, 239, 895, 1666, 1674, 1808
Hpy99I CGWCG 2 cut(s) 173, 901
HpyAV CCTTC 8 cut(s) 257, 307, 555, 571, 809, 871, 1735, 1814
HpyCH4III ACNGT 7 cut(s) 101, 230, 494, 518, 775, 1273, 1410
HpyCH4IV ACGT 2 cut(s) 378, 1517
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 1260, 1550, 1655, 1976
HpyF3I CTNAG 2 cut(s) 17, 1630
HpySE526I ACGT 2 cut(s) 378, 1517
Hsp92II CATG 5 cut(s) 26, 609, 724, 806, 1721
HspAI GCGC 2 cut(s) 1349, 1528
KpnI GGTACC 1 cut(s) 498
Kzo9I GATC 4 cut(s) 291, 414, 838, 1676
LguI GCTCTTC 1 cut(s) 980
LmnI GCTCC 3 cut(s) 1056, 1147, 1556
Lsp1109I GCAGC 5 cut(s) 33, 321, 515, 1528, 1592
LweI GCATC 5 cut(s) 74, 443, 959, 1912, 1976
MaeI CTAG 4 cut(s) 56, 570, 938, 1868
MaeII ACGT 2 cut(s) 378, 1517
MalI GATC 4 cut(s) 293, 416, 840, 1678
MboI GATC 4 cut(s) 291, 414, 838, 1676
MboII GAAGA 7 cut(s) 818, 883, 997, 1123, 1311, 1907, 1964
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 1 cut(s) 1561
MlsI TGGCCA 3 cut(s) 410, 1220, 1254
MluNI TGGCCA 3 cut(s) 410, 1220, 1254
MmeI TCCRAC 1 cut(s) 2022
Mox20I TGGCCA 3 cut(s) 410, 1220, 1254
MroXI GAANNNNTTC 3 cut(s) 380, 639, 1115
MscI TGGCCA 3 cut(s) 410, 1220, 1254
MslI CAYNNNNRTG 1 cut(s) 1395
Msp20I TGGCCA 3 cut(s) 410, 1220, 1254
MspCI CTTAAG 1 cut(s) 1531
MspI CCGG 1 cut(s) 795
MspR9I CCNGG 3 cut(s) 499, 1063, 1256
Mva1269I GAATGC 2 cut(s) 617, 919
MvaI CCWGG 3 cut(s) 499, 1063, 1256
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 5 cut(s) 46, 1260, 1550, 1655, 1976
NdeII GATC 4 cut(s) 291, 414, 838, 1676
NlaIII CATG 5 cut(s) 26, 609, 724, 806, 1721
NlaIV GGNNCC 7 cut(s) 179, 496, 751, 840, 1060, 1723, 1740
NmuCI GTSAC 4 cut(s) 191, 280, 467, 1848
NsbI TGCGCA 1 cut(s) 1350
NspI RCATGY 1 cut(s) 724
PaeR7I CTCGAG 1 cut(s) 1926
PceI AGGCCT 1 cut(s) 1866
PciI ACATGT 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 980
PcsI WCGNNNNNNNCGW 1 cut(s) 2153
PctI GAATGC 2 cut(s) 617, 919
PdmI GAANNNNTTC 3 cut(s) 380, 639, 1115
PfeI GAWTC 6 cut(s) 949, 1038, 1302, 1900, 2050, 2189
PflFI GACNNNGTC 1 cut(s) 2152
PkrI GCNGC 6 cut(s) 48, 311, 530, 1162, 1543, 1582
Ppu21I YACGTR 1 cut(s) 1518
PpuMI RGGWCCY 2 cut(s) 244, 750
PscI ACATGT 1 cut(s) 720
PshAI GACNNNNGTC 1 cut(s) 902
Psp124BI GAGCTC 1 cut(s) 1561
Psp5II RGGWCCY 2 cut(s) 244, 750
Psp6I CCWGG 3 cut(s) 497, 1061, 1254
PspEI GGTNACC 1 cut(s) 1848
PspGI CCWGG 3 cut(s) 497, 1061, 1254
PspN4I GGNNCC 7 cut(s) 179, 496, 751, 840, 1060, 1723, 1740
PspPI GGNCC 4 cut(s) 244, 750, 1738, 2129
PspPPI RGGWCCY 2 cut(s) 244, 750
PspXI VCTCGAGB 1 cut(s) 1926
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 1 cut(s) 2152
RseI CAYNNNNRTG 1 cut(s) 1395
SacI GAGCTC 1 cut(s) 1561
SapI GCTCTTC 1 cut(s) 980
SatI GCNGC 6 cut(s) 47, 310, 529, 1161, 1542, 1581
Sau3AI GATC 4 cut(s) 291, 414, 838, 1676
Sau96I GGNCC 4 cut(s) 244, 750, 1738, 2129
ScaI AGTACT 1 cut(s) 1748
ScrFI CCNGG 3 cut(s) 499, 1063, 1256
SduI GDGCHC 1 cut(s) 1561
SfaNI GCATC 5 cut(s) 74, 443, 959, 1912, 1976
SfcI CTRYAG 1 cut(s) 387
SfiI GGCCNNNNNGGCC 1 cut(s) 1260
Sfr274I CTCGAG 1 cut(s) 1926
SinI GGWCC 4 cut(s) 244, 750, 1738, 2129
SlaI CTCGAG 1 cut(s) 1926
SmiMI CAYNNNNRTG 1 cut(s) 1395
SmlI CTYRAG 4 cut(s) 843, 1531, 1808, 1926
SmoI CTYRAG 4 cut(s) 843, 1531, 1808, 1926
SpeI ACTAGT 1 cut(s) 569
SseBI AGGCCT 1 cut(s) 1866
SsiI CCGC 6 cut(s) 49, 80, 328, 631, 863, 1160
SspI AATATT 1 cut(s) 697
SspMI CTAG 4 cut(s) 56, 570, 938, 1868
SstI GAGCTC 1 cut(s) 1561
StuI AGGCCT 1 cut(s) 1866
StyD4I CCNGG 3 cut(s) 497, 1061, 1254
StyI CCWWGG 3 cut(s) 1054, 1264, 2132
TaaI ACNGT 7 cut(s) 101, 230, 494, 518, 775, 1273, 1410
TaiI ACGT 2 cut(s) 381, 1520
TaqI TCGA 6 cut(s) 249, 896, 1239, 1927, 2103, 2147
TatI WGTACW 2 cut(s) 1714, 1746
TauI GCSGC 1 cut(s) 1163
TfiI GAWTC 6 cut(s) 949, 1038, 1302, 1900, 2050, 2189
TscAI CASTG 2 cut(s) 391, 1033
TseFI GTSAC 4 cut(s) 191, 280, 467, 1848
TseI GCWGC 5 cut(s) 46, 309, 528, 1541, 1580
Tsp45I GTSAC 4 cut(s) 191, 280, 467, 1848
TspDTI ATGAA 9 cut(s) 537, 594, 791, 1345, 1447, 1467, 1554, 2036, 2133
TspGWI ACGGA 7 cut(s) 819, 914, 981, 1307, 1784, 1822, 2169
TspRI CASTG 2 cut(s) 391, 1033
Tth111I GACNNNGTC 1 cut(s) 2152
Vha464I CTTAAG 1 cut(s) 1531
VpaK11BI GGWCC 4 cut(s) 244, 750, 1738, 2129
XapI RAATTY 4 cut(s) 252, 1245, 1454, 2089
XceI RCATGY 1 cut(s) 724
XhoI CTCGAG 1 cut(s) 1926
XmiI GTMKAC 3 cut(s) 627, 1138, 1616
XmnI GAANNNNTTC 3 cut(s) 380, 639, 1115
XspI CTAG 4 cut(s) 56, 570, 938, 1868
ZrmI AGTACT 1 cut(s) 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.