Rw2G040880

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000052
Physical Location & Seq
Reverse (-)
66553291 .. 66555853
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040880.1

Sequence Viewer

Length: 2169 bp
ATGCAATCCCTAGTTCATCATATGATATCTATTATGGTTTTATGTTTATGGAGCTTGAGTACTACACCAGCAGTAGCAGCAGAATCAGGAACAGCGCTGCCGATAGCAAAGCCGAATTGCCAAGAGAAATGTGGAAATGTCAGCATCCCGTACCCTTTCGGGATCGGGCCTGATAAAGATTGCTACCTTGAAGAGTGGTTTGAAATAGACTGCAACCAGTCTACTAGGCACAAGCCTTTCTTGAGGCTTACACAACAGGAGGTACTGAGCATTTCTATCAAAGACGGCACGCTTCAGGTGAACAGCCCCGTTACTTTCTTCTGCAACGTAACAGGAAGTAGCCAGCCCGCAAATCTGACCGGAAGCCCTTTCGTGTACTCTCAGAGACAAAACAGATTCACCGCAGCGACTTGCGGCTTTGTCTCCTTGGTGAGCTCAGATCAATCGGTGGTTGGTGGGTGCAGGTCAATTTGTGATAAGCAGAGTACCGGTAGATACTCTGATGGCTGTGATATTGGTACTAATTGTTGCCAGACGAGTATTCCTCCGTATCTCAGGGTTGTCACTGCTAGCGTAATGGTGGATGGACCAGCCAACATGACCGGCCGAAATGACAGTGGAAAGCCGGATTGTAGTGACTACGCATTCCTGGTTGATAAAGATTGGTTTGAGACTGCATCAAACTATAGCGCCATTAGGAATATGCTCCATGTTCCTGTGGCACTAGAATGGAGTGTAATCAATGACACTAATTCGTCGTCTGCATTTCAAGGAAATAAGAAATGCGATTTTGATACTTCTTTGGCACTTAATCGGTCAGTGCTTTCTTGCCGCTGCCCACAAGGGTTCGATGGAAATCCTTATCTTCTCCAACCTTGTCAAGACATTGATGAGTGCATAGATGGTACTAAACGATGCTGGCCCGGCAGCAAGTGCATGAACCTTCCGGGTTCGTTCAAATGCGAGGACGAAAAGGATACAATTAAATTGGCACTTGCAGGTCTTGGCTCGAGTCTTGGACTGTTATTATTACTTATTGCTGCATGGTGGGTACACAAACTGGTGAAGAAAAGGAAAAGCATTAAACGCAAGGAAATGTCATTCAAAAGAAACGGAGGTTTGTTGTTAGAAAGACAGTTATCATCTGGTGAAGTTAATCTTGAGAAAATCAAGTTGTTCAAATCAAAAGAGTTGGAGAAGTCCACTGATAATTTTCATGTTGATAGAATCCTTGGCAAAGGTGGCCAAGGGACTGTCTACAAAGGTATGCTAACAGATGGAAGAATCGTTGCAGTAAAAAGGTCTACAAAAGTGGACGAAGGTAGACTTTCACAGTTTATAAATGAGGTTGTTATTCTTTCACAAATCAACCACAGGAATGTGTTTATTCCGAATGGATCCCTTTCCCAATATATCCATGAGCAGCACGAGGATTTTCCACTTACGTGGGAATTGCGCTTAAGGATTGCCACGGAAATTGCAGGAGCCCTTTCCTACTTACACGGTGCAGCTTCAATTCCTATATTTCATAGAGACATCAAGTCGACAAATATATTGCTAGATGAAAAATACAGGGCAAAAATTGCCGACTTTGGAACTTCTAGATCAGTTGCCATAGACCAAACTCACTTGACCACACTTGTATATGGGACATTTGGTTACTTGGACCCAGAATACTTCCAGTCGAGCCAATTTACGGATAAAAGTGATGTGTATAGCTTTGGAGTGGTCCTTGTTGAACTCTTGAGTGGACAAAAACCAATCTCTGTGACAAGATCACAAGAAGAAGGTCGGAGTCTTGCAACACATTTCATTATTTTGATGCAGGATGATCGGCTATTTGAAGTTGTTGATACTCGAGTTTTGAATGAAGCCTCTAAAGAAGATATCTTACTAGTTGCCAACCTTGCAAGAAGATGTTTGCATCTGAATGGTAGGAACCGCCCCACAATGAGAGAGGTAACAGCAGAGCTGGAGGGGATTCAAATGTCACAAAAAACAACTAATGGTGAGCAAAATTATGAAGAGGTTGATATTGTCAGGTATGATTCAATTGAGCCATGGGATGTTGCTTCAACTTCAACAGGGACAGTAACAGGAACAAGGTCAGGTGTTGCTGCTGGTCCGTCTTCATCACCATCGCAAGGAATTGCACTGTTATCTTTGTAG

Protein Analysis

722

Amino Acids

79.78

Weight (kDa)

6.31

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 101 1.2e-13 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 322 2.6e-06 Calcium-binding EGF domain
Pkinase PF00069 405 - 461 2.1e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 406 - 658 2.9e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 464 - 655 8.4e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
Acc36I ACCTGC 2 cut(s) 453, 989
AccI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1544
AciI CCGC 5 cut(s) 348, 402, 414, 832, 1942
AclWI GGATC 3 cut(s) 170, 1392, 1405
AcoI YGGCCR 2 cut(s) 604, 1243
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 8 cut(s) 61, 152, 264, 377, 487, 520, 907, 1053
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 2 cut(s) 1696, 2144
AflII CTTAAG 1 cut(s) 1459
AgeI ACCGGT 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 1540
AhlI ACTAGT 1 cut(s) 1894
AjnI CCWGG 1 cut(s) 648
AleI CACNNNNGTG 1 cut(s) 1444
AluBI AGCT 5 cut(s) 54, 435, 1511, 1719, 1972
AluI AGCT 5 cut(s) 54, 435, 1511, 1719, 1972
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 4 cut(s) 379, 427, 665, 1527
AlwI GGATC 3 cut(s) 170, 1392, 1405
Ama87I CYCGRG 2 cut(s) 1009, 1857
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 4 cut(s) 167, 604, 920, 1243
ApeKI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1423, 1508, 2117
AsiGI ACCGGT 1 cut(s) 488
AspLEI GCGC 3 cut(s) 97, 692, 1458
AspS9I GGNCC 6 cut(s) 167, 587, 921, 1666, 1729, 2123
AsuC2I CCSGG 2 cut(s) 924, 948
AsuHPI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2021, 2127
AsuNHI GCTAGC 1 cut(s) 569
AvaI CYCGRG 2 cut(s) 1009, 1857
AvaII GGWCC 4 cut(s) 587, 1666, 1729, 2123
BaeI ACNNNNGTAYC 2 cut(s) 1845, 1878
BalI TGGCCA 1 cut(s) 1245
BamHI GGATCC 1 cut(s) 1397
BanII GRGCYC 2 cut(s) 437, 1489
BarI GAAGNNNNNNTAC 2 cut(s) 1875, 1907
BauI CACGAG 1 cut(s) 1427
BbsI GAAGAC 1 cut(s) 2121
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1435, 1520, 2104
BccI CCATC 6 cut(s) 497, 578, 845, 896, 1271, 2146
BceAI ACGGC 1 cut(s) 301
BcgI CGANNNNNNTGC 2 cut(s) 1814, 1848
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 1 cut(s) 970
BcnI CCSGG 2 cut(s) 924, 948
BcoDI GTCTC 4 cut(s) 379, 427, 665, 1527
BcuI ACTAGT 1 cut(s) 1894
BfaI CTAG 7 cut(s) 11, 225, 570, 725, 1559, 1602, 1895
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 2 cut(s) 98, 693
BfrI CTTAAG 1 cut(s) 1459
BfuAI ACCTGC 2 cut(s) 453, 989
BfuI GTATCC 1 cut(s) 970
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 3 cut(s) 650, 924, 948
Bme18I GGWCC 4 cut(s) 587, 1666, 1729, 2123
BmeRI GACNNNNNGTC 1 cut(s) 1540
BmeT110I CYCGRG 2 cut(s) 1009, 1857
BmgT120I GGNCC 6 cut(s) 167, 587, 921, 1666, 1729, 2123
BmiI GGNNCC 4 cut(s) 1399, 1486, 1668, 1940
BmrFI CCNGG 3 cut(s) 650, 924, 948
BmsI GCATC 5 cut(s) 153, 686, 905, 1812, 1933
BmtI GCTAGC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 2121
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpmI CTGGAG 1 cut(s) 1994
BpuEI CTTGAG 4 cut(s) 76, 262, 1181, 1765
BpuMI CCSGG 2 cut(s) 924, 948
BsaAI YACGTR 1 cut(s) 1446
BsaBI GATNNNNATC 1 cut(s) 855
BsaJI CCNNGG 5 cut(s) 426, 1231, 1246, 1470, 2060
BsaWI WCCGGW 2 cut(s) 359, 488
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 2 cut(s) 1696, 2144
Bse118I RCCGGY 2 cut(s) 488, 602
Bse1I ACTGG 3 cut(s) 217, 1065, 1681
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 5 cut(s) 426, 1231, 1246, 1470, 2060
BseGI GGATG 4 cut(s) 144, 589, 1834, 2071
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 2 cut(s) 1696, 2144
BseMII CTCAG 4 cut(s) 257, 395, 450, 568
BseNI ACTGG 3 cut(s) 217, 1065, 1681
BseX3I CGGCCG 1 cut(s) 604
BseXI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1435, 1520, 2104
BsgI GTGCAG 2 cut(s) 481, 1527
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 4 cut(s) 169, 606, 922, 1245
BshTI ACCGGT 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 2 cut(s) 1009, 1857
BsiSI CCGG 6 cut(s) 360, 489, 603, 626, 924, 947
BslFI GGGAC 3 cut(s) 1264, 1663, 2101
BslI CCNNNNNNNGG 2 cut(s) 1696, 2144
BsmAI GTCTC 4 cut(s) 379, 427, 665, 1527
BsmFI GGGAC 3 cut(s) 1264, 1663, 2101
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 169, 606, 922, 1245
BsoBI CYCGRG 2 cut(s) 1009, 1857
Bsp1286I GDGCHC 2 cut(s) 437, 1489
Bsp143I GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
Bsp19I CCATGG 1 cut(s) 2060
BspACI CCGC 5 cut(s) 348, 402, 414, 832, 1942
BspANI GGCC 4 cut(s) 169, 606, 922, 1245
BspCNI CTCAG 4 cut(s) 258, 394, 449, 567
BspLI GGNNCC 4 cut(s) 1399, 1486, 1668, 1940
BspMI ACCTGC 2 cut(s) 453, 989
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 3 cut(s) 170, 1392, 1405
BspTI CTTAAG 1 cut(s) 1459
BsrFI RCCGGY 2 cut(s) 488, 602
BsrI ACTGG 3 cut(s) 217, 1065, 1681
BssAI RCCGGY 2 cut(s) 488, 602
BssECI CCNNGG 5 cut(s) 426, 1231, 1246, 1470, 2060
BssMI GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
BssSI CACGAG 1 cut(s) 1427
BssT1I CCWWGG 4 cut(s) 426, 1231, 1246, 2060
Bst2BI CACGAG 1 cut(s) 1427
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1505, 2092, 2157
Bst6I CTCTTC 2 cut(s) 186, 2019
BstAFI CTTAAG 1 cut(s) 1459
BstAPI GCANNNNNTGC 2 cut(s) 933, 1583
BstBAI YACGTR 1 cut(s) 1446
BstC8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
BstDEI CTNAG 4 cut(s) 266, 381, 436, 554
BstDSI CCRYGG 2 cut(s) 1470, 2060
BstF5I GGATG 4 cut(s) 144, 589, 1834, 2071
BstH2I RGCGCY 2 cut(s) 98, 693
BstHHI GCGC 3 cut(s) 97, 692, 1458
BstKTI GATC 6 cut(s) 165, 442, 1400, 1607, 1778, 1834
BstMAI GTCTC 4 cut(s) 379, 427, 665, 1527
BstMBI GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1583, 1907
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 648, 922, 946
BstSFI CTRYAG 1 cut(s) 685
BstV1I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1435, 1520, 2104
BstV2I GAAGAC 1 cut(s) 2121
BstX2I RGATCY 1 cut(s) 1397
BstXI CCANNNNNNTGG 1 cut(s) 1446
BstYI RGATCY 1 cut(s) 1397
BstZI CGGCCG 1 cut(s) 604
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 4 cut(s) 169, 606, 922, 1245
BtgI CCRYGG 2 cut(s) 1470, 2060
BtgZI GCGATG 1 cut(s) 2124
BtsCI GGATG 4 cut(s) 144, 589, 1834, 2071
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 5 cut(s) 564, 622, 825, 1203, 2153
BveI ACCTGC 2 cut(s) 453, 989
Cac8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
CfoI GCGC 3 cut(s) 97, 692, 1458
Cfr10I RCCGGY 2 cut(s) 488, 602
Cfr13I GGNCC 6 cut(s) 167, 587, 921, 1666, 1729, 2123
Csp6I GTAC 8 cut(s) 60, 151, 263, 376, 486, 519, 906, 1052
CspAI ACCGGT 1 cut(s) 488
CspCI CAANNNNNGTGG 2 cut(s) 1459, 1494
CviAII CATG 7 cut(s) 598, 710, 937, 1044, 1217, 1418, 2061
CviQI GTAC 8 cut(s) 60, 151, 263, 376, 486, 519, 906, 1052
DdeI CTNAG 4 cut(s) 266, 381, 436, 554
DpnI GATC 6 cut(s) 164, 441, 1399, 1606, 1777, 1833
DpnII GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
DriI GACNNNNNGTC 1 cut(s) 1540
EaeI YGGCCR 2 cut(s) 604, 1243
EagI CGGCCG 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 186, 2019
Eam1105I GACNNNNNGTC 1 cut(s) 1540
EarI CTCTTC 2 cut(s) 186, 2019
Ecl136II GAGCTC 1 cut(s) 435
EclXI CGGCCG 1 cut(s) 604
Eco130I CCWWGG 4 cut(s) 426, 1231, 1246, 2060
Eco24I GRGCYC 2 cut(s) 437, 1489
Eco32I GATATC 2 cut(s) 27, 1888
Eco47I GGWCC 4 cut(s) 587, 1666, 1729, 2123
Eco47III AGCGCT 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 1009, 1857
EcoICRI GAGCTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 2 cut(s) 27, 1888
EcoT14I CCWWGG 4 cut(s) 426, 1231, 1246, 2060
EcoT38I GRGCYC 2 cut(s) 437, 1489
ErhI CCWWGG 4 cut(s) 426, 1231, 1246, 2060
FaeI CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1421, 2064
FalI AAGNNNNNCTT 6 cut(s) 224, 256, 1143, 1175, 1311, 1343
FaqI GGGAC 3 cut(s) 1264, 1663, 2101
FatI CATG 7 cut(s) 597, 709, 936, 1043, 1216, 1417, 2060
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1544
FokI GGATG 4 cut(s) 131, 596, 1841, 2078
FriOI GRGCYC 2 cut(s) 437, 1489
FspBI CTAG 7 cut(s) 11, 225, 570, 725, 1559, 1602, 1895
GlaI GCGC 3 cut(s) 96, 691, 1457
GsuI CTGGAG 1 cut(s) 1994
HaeII RGCGCY 2 cut(s) 98, 693
HaeIII GGCC 4 cut(s) 169, 606, 922, 1245
HapII CCGG 6 cut(s) 360, 489, 603, 626, 924, 947
HhaI GCGC 3 cut(s) 97, 692, 1458
Hin1II CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1421, 2064
Hin6I GCGC 3 cut(s) 95, 690, 1456
HinP1I GCGC 3 cut(s) 95, 690, 1456
HincII GTYRAC 1 cut(s) 1545
HindII GTYRAC 1 cut(s) 1545
HinfI GANTC 8 cut(s) 83, 396, 1012, 1227, 1284, 1795, 1981, 2048
HpaII CCGG 6 cut(s) 360, 489, 603, 626, 924, 947
HphI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2021, 2127
Hpy188I TCNGA 7 cut(s) 357, 384, 439, 502, 1392, 1794, 1929
Hpy188III TCNNGA 7 cut(s) 87, 160, 241, 881, 1160, 1602, 1744
Hpy99I CGWCG 1 cut(s) 760
HpyAV CCTTC 3 cut(s) 953, 1313, 1781
HpyCH4III ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1505, 2092, 2157
HpyCH4IV ACGT 2 cut(s) 327, 1445
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1583, 1907
HpyF3I CTNAG 4 cut(s) 266, 381, 436, 554
HpySE526I ACGT 2 cut(s) 327, 1445
Hsp92II CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1421, 2064
HspAI GCGC 3 cut(s) 95, 690, 1456
Kzo9I GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
LmnI GCTCC 3 cut(s) 51, 711, 1484
Lsp1109I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1435, 1520, 2104
LweI GCATC 5 cut(s) 153, 686, 905, 1812, 1933
MaeI CTAG 7 cut(s) 11, 225, 570, 725, 1559, 1602, 1895
MaeII ACGT 2 cut(s) 327, 1445
MaeIII GTNAC 9 cut(s) 310, 328, 562, 635, 1658, 1768, 1960, 1989, 2092
MalI GATC 6 cut(s) 164, 441, 1399, 1606, 1777, 1833
MboI GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
MfeI CAATTG 1 cut(s) 2052
MflI RGATCY 1 cut(s) 1397
MhlI GDGCHC 2 cut(s) 437, 1489
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 2 cut(s) 1021, 1804
MmeI TCCRAC 3 cut(s) 895, 1173, 1772
Mox20I TGGCCA 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 5 cut(s) 810, 984, 1083, 1155, 1460
MslI CAYNNNNRTG 4 cut(s) 727, 1377, 1444, 1929
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 1 cut(s) 834
MspCI CTTAAG 1 cut(s) 1459
MspI CCGG 6 cut(s) 360, 489, 603, 626, 924, 947
MspR9I CCNGG 3 cut(s) 650, 924, 948
MunI CAATTG 1 cut(s) 2052
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1583, 1907
NciI CCSGG 2 cut(s) 924, 948
NcoI CCATGG 1 cut(s) 2060
NdeI CATATG 1 cut(s) 21
NdeII GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1421, 2064
NlaIV GGNNCC 4 cut(s) 1399, 1486, 1668, 1940
NmuCI GTSAC 4 cut(s) 562, 635, 1768, 1989
OliI CACNNNNGTG 1 cut(s) 1444
PaeR7I CTCGAG 2 cut(s) 1009, 1857
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 6 cut(s) 83, 396, 1227, 1284, 1981, 2048
PinAI ACCGGT 1 cut(s) 488
PleI GAGTC 2 cut(s) 1020, 1803
PpsI GAGTC 2 cut(s) 1020, 1803
Ppu21I YACGTR 1 cut(s) 1446
PsiI TTATAA 1 cut(s) 1340
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 1399, 1486, 1668, 1940
PspPI GGNCC 6 cut(s) 167, 587, 921, 1666, 1729, 2123
PspXI VCTCGAGB 2 cut(s) 1009, 1857
PsuI RGATCY 1 cut(s) 1397
RsaI GTAC 8 cut(s) 61, 152, 264, 377, 487, 520, 907, 1053
RsaNI GTAC 8 cut(s) 60, 151, 263, 376, 486, 519, 906, 1052
RseI CAYNNNNRTG 4 cut(s) 727, 1377, 1444, 1929
SacI GAGCTC 1 cut(s) 437
SalI GTCGAC 1 cut(s) 1543
SaqAI TTAA 5 cut(s) 810, 984, 1083, 1155, 1460
Sau3AI GATC 6 cut(s) 162, 439, 1397, 1604, 1775, 1831
Sau96I GGNCC 6 cut(s) 167, 587, 921, 1666, 1729, 2123
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 2 cut(s) 1021, 1804
ScrFI CCNGG 3 cut(s) 650, 924, 948
SduI GDGCHC 2 cut(s) 437, 1489
SfaNI GCATC 5 cut(s) 153, 686, 905, 1812, 1933
SfcI CTRYAG 1 cut(s) 685
Sfr274I CTCGAG 2 cut(s) 1009, 1857
SinI GGWCC 4 cut(s) 587, 1666, 1729, 2123
SlaI CTCGAG 2 cut(s) 1009, 1857
SmiMI CAYNNNNRTG 4 cut(s) 727, 1377, 1444, 1929
SmlI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1459, 1744, 1857
SmoI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1459, 1744, 1857
SpeI ACTAGT 1 cut(s) 1894
SsiI CCGC 5 cut(s) 348, 402, 414, 832, 1942
SspMI CTAG 7 cut(s) 11, 225, 570, 725, 1559, 1602, 1895
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 648, 922, 946
StyI CCWWGG 4 cut(s) 426, 1231, 1246, 2060
TaaI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1505, 2092, 2157
TaiI ACGT 2 cut(s) 330, 1448
TaqI TCGA 5 cut(s) 849, 1010, 1544, 1685, 1858
TaqII GACCGA 1 cut(s) 804
TatI WGTACW 2 cut(s) 59, 375
TauI GCSGC 2 cut(s) 417, 834
TfiI GAWTC 6 cut(s) 83, 396, 1227, 1284, 1981, 2048
Tru1I TTAA 5 cut(s) 810, 984, 1083, 1155, 1460
Tru9I TTAA 5 cut(s) 810, 984, 1083, 1155, 1460
TscAI CASTG 5 cut(s) 571, 622, 825, 1210, 2160
TseFI GTSAC 4 cut(s) 562, 635, 1768, 1989
TseI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1423, 1508, 2117
Tsp45I GTSAC 4 cut(s) 562, 635, 1768, 1989
TspDTI ATGAA 9 cut(s) 5, 953, 1205, 1517, 1578, 1801, 1884, 2037, 2121
TspGWI ACGGA 5 cut(s) 537, 1128, 1487, 1712, 2115
TspRI CASTG 5 cut(s) 571, 622, 825, 1210, 2160
Vha464I CTTAAG 1 cut(s) 1459
VpaK11BI GGWCC 4 cut(s) 587, 1666, 1729, 2123
XbaI TCTAGA 1 cut(s) 1601
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XhoI CTCGAG 2 cut(s) 1009, 1857
XmiI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1544
XspI CTAG 7 cut(s) 11, 225, 570, 725, 1559, 1602, 1895
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.