Rh3DG199400

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000052
Physical Location & Seq
Reverse (-)
18041729 .. 18049188
7460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG199400.1

Sequence Viewer

Length: 2337 bp
ATGCAATGGCTAGTTCAGCGCATGATTTTCATTCTATTGTTATATAAATGGCGCATAAGTACTGCATCATCATCACCACTGAAGATAGCGAAGGAGCATTGTGCATCGCAGTGCGGAAATGTTAGCATCCCATACCCTTTCGGGATTGGACGTCCTAATAACCATTGTTATTTTGACGAGTGGTACGAAATAGAATGCAACCCTTCTTTTCCAGTACCGAAGCCATTCTTGAGGCGTCTACAACTGGAGGTGCTGAATATTTCTGTTGAAAACGCCACGCTTCAGGTTACAAGCCCTATCACTTACTTTAGCTGCAAGGGGAAGCAATCTCGGCCAGCGGCAAATCTGACGGGAAGCCCTTTTATGTACTCTCAACGAATTAACAGTTTCGTTGCAGTTAGTTGTGACTTATTTGCCTCGCTGCGCACGGGTTCAAAGCACACTGTTGCTGGGTGCACGTCAACTTGTACTGCATATGCATTTCAGGGTCTTGATATGTGTGAGTATGGCTTAAACTGTTGCCGGACTGCCCTTTCCCAATATATCAATAATTTCAGCGTCAAACAAGAACCAGATGAAACAAGAAGAAATGCGACCGATTGCGAGGACTATGCATTCCTGGTTGACAAACAGTGGTTTGAAGACAATAAATCAAATTTCATAGCTATCAAGGGTATGGACGTCGTTCCTGTGATGCTAGACTGGAGCTTAATTTTAGAAAATAATAAGTCATTAATCAAACAATTTGAAGCACTCCCCAGCTTGCCAGATAGGAGCCCGCATGACTCAACACCCACATGCGACAAAATTGGTGCTGTTCCGTCTTTCAATCACACATGGTATCAATGTCTTTGTCCGGCAGGCTTTGAAGGAAATCCGTACCTTCTCCAACGTTGTCAAGATATAGATGAATGCAAGCATAGTAATCCGTGTGCAAGTCTTGACCCTAATATTACTACTGCATGGAATATTAAAGATGCCAAATGTAACAACACTGTCGGAGGTCATACATGCTACTCAGATAGAACAGGGGAAACCTGCGAATTTTTGGCCATTGACCCCGTTGCCAAGTGCTACAAAAGAAGTATGCCTCATTCACAGCTCAAGCCTATTCTCTATGGTCTTGGTGCTGGTATTGGACTATTGTTGTTGCTTAGTGCTGCATGGTGGGTGTACAAGTTTGTAAAAAAATGGAAAAACATTAAACGTAAGGAGATGTTTTACAAACGAAATGGTGGTTTATTGCTAGAGCAACAATTATCGTCAGGTGACATTAACATTGAGAGAATCAAATTGTTCAAATCGAAGGAGTTACAGAGGTCTACAAACAATTTCAATGCTGACAGAATTATTGGCCAAGGGGGGCAAGGTACTGTTTACAAAGGCATGTTGATGGATGGAAGAATTGTGGCTGTAAAAAAGTCAAAAATTGTAGACAAGGCAAAACTTTCAGAATTCATCAATGAGGTTGTCATTCTTTCTCAAATCAACCACCGGAATATCGTTCAACTATTGGGTTGTTGCCTAGAGACGGAAGTTCCTCTTTTGGTATATGAATTTATACCTAATGGAAACTTATCTCAGTACATCCATGAGCAGACTGAAGAATTCCCACTTACATGGGAGATTCGTTTACGAATTGTTACAGAAATTGCAGGAGCTCTTTTCTACTTACATGCTTCAGCTTTGTTTCCCATTTATCACAGAGACATCAAGTCCACAAATATACTCTTGGATGATAAATACAGAGCAAAAATTGCTGACTTTGGAACTTCAAGATTAGTTGCCATTGACCAAACTCACCTGACCACAAACGTACATGGAACATTTGGTTATTTGGATCCTGAATACTACCAATCGAGTCAATTTACTGAGAAAAGTGACGTCTACAGCTTTGGAGTGGTGCTTGTTGAGCTCTTGACCGGAAAAAAACCAGTAACAAGGTCAGCCGAAGACGAAACATACAAGAGTCTTGCCACATATTTTATTGTTTCGTTGCAGGAAGATTCTCTATTTGACATTCTTGATGCTCGAGTTGTGAATGAAGGCTCTAAACATGGTATTATGGTAGTCGCTAATCTTGCAAAACGATGTTTAAATTTGAATGGAAGAAAGCGCCCTACGATGAGAGAGATCACAGCAGAGTTGGAGGCCATCCAATTATCGGAGAAACTTGCCGACGCTCGTCAACCAAGTTGTGAAAGGATTGAATTTGGCCAAGATTATCGAATCGAGCAGTGGGATGAGGTTGTTTCTAGCTCCACAGTGTCAACTTGGGAAGATGCTCCGACTCCATCAACAGTTGAACTTCCACTAGTTTCTATGAAGACATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

778

Amino Acids

88.02

Weight (kDa)

6.74

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 97 4.7e-12 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 446 - 713 3.5e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 447 - 716 6.7e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 154, 684, 1887
Acc16I TGCGCA 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 1046
AccI GTMKAC 4 cut(s) 238, 1322, 1434, 1887
AciI CCGC 3 cut(s) 114, 338, 779
AclI AACGTT 1 cut(s) 892
AclWI GGATC 2 cut(s) 1835, 1848
AcoI YGGCCR 4 cut(s) 332, 1050, 1354, 2215
AcsI RAATTY 7 cut(s) 655, 1043, 1454, 1556, 1607, 2098, 2210
AcuI CTGAAG 4 cut(s) 101, 266, 1623, 1665
AcyI GRCGYC 4 cut(s) 151, 235, 681, 1884
AfiI CCNNNNNNNGG 2 cut(s) 1531, 2164
AflIII ACRYGT 1 cut(s) 2330
AhdI GACNNNNNGTC 1 cut(s) 1714
AhlI ACTAGT 1 cut(s) 2314
AjiI CACGTC 1 cut(s) 459
AjnI CCWGG 1 cut(s) 618
Alw21I GWGCWC 3 cut(s) 458, 1663, 1917
Alw26I GTCTC 2 cut(s) 1523, 1701
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 2 cut(s) 1835, 1848
Ama87I CYCGRG 1 cut(s) 2031
AoxI GGCC 5 cut(s) 332, 1050, 1354, 2151, 2215
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 3 cut(s) 312, 421, 1160
ApoI RAATTY 7 cut(s) 655, 1043, 1454, 1556, 1607, 2098, 2210
AseI ATTAAT 1 cut(s) 734
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 4 cut(s) 21, 54, 426, 2118
AsuHPI GGTGA 3 cut(s) 66, 1280, 1793
AvaI CYCGRG 1 cut(s) 2031
BaeGI GKGCMC 1 cut(s) 458
BalI TGGCCA 3 cut(s) 1052, 1356, 2217
BamHI GGATCC 1 cut(s) 1840
BanII GRGCYC 3 cut(s) 779, 1663, 1917
BbsI GAAGAC 3 cut(s) 648, 1959, 2333
Bbv12I GWGCWC 3 cut(s) 458, 1663, 1917
BbvI GCAGC 3 cut(s) 299, 408, 1147
BccI CCATC 4 cut(s) 1387, 1391, 2162, 2302
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 1523, 1701
BcuI ACTAGT 1 cut(s) 2314
BfaI CTAG 6 cut(s) 11, 698, 1247, 1526, 2256, 2315
BfmI CTRYAG 1 cut(s) 1888
BfoI RGCGCY 1 cut(s) 2119
BfuAI ACCTGC 1 cut(s) 1046
BisI GCNGC 4 cut(s) 313, 339, 422, 1161
BlsI GCNGC 4 cut(s) 314, 340, 423, 1162
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 620
BmeRI GACNNNNNGTC 1 cut(s) 1714
BmeT110I CYCGRG 1 cut(s) 2031
BmgBI CACGTC 1 cut(s) 459
BmiI GGNNCC 2 cut(s) 776, 1842
BmrFI CCNGG 1 cut(s) 620
BmsI GCATC 7 cut(s) 74, 113, 135, 684, 967, 2017, 2272
BoxI GACNNNNGTC 1 cut(s) 2184
BpiI GAAGAC 3 cut(s) 648, 1959, 2333
BpmI CTGGAG 2 cut(s) 266, 724
BpuEI CTTGAG 2 cut(s) 250, 1088
BsaHI GRCGYC 4 cut(s) 151, 235, 681, 1884
BsaJI CCNNGG 1 cut(s) 1357
BsaWI WCCGGW 2 cut(s) 1494, 1922
Bsc4I CCNNNNNNNGG 2 cut(s) 1531, 2164
Bse1I ACTGG 4 cut(s) 212, 249, 707, 1934
Bse3DI GCAATG 1 cut(s) 11
BseBI CCWGG 1 cut(s) 620
BseDI CCNNGG 1 cut(s) 1357
BseGI GGATG 6 cut(s) 126, 1402, 1587, 1741, 2154, 2248
BseLI CCNNNNNNNGG 2 cut(s) 1531, 2164
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 3 cut(s) 1032, 1595, 1863
BseNI ACTGG 4 cut(s) 212, 249, 707, 1934
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 3 cut(s) 299, 408, 1147
BseYI CCCAGC 2 cut(s) 449, 758
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 5 cut(s) 334, 1052, 1356, 2153, 2217
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 3 cut(s) 458, 1663, 1917
BsiHKCI CYCGRG 1 cut(s) 2031
BsiSI CCGG 4 cut(s) 523, 857, 1495, 1923
BslI CCNNNNNNNGG 2 cut(s) 1531, 2164
BsmAI GTCTC 2 cut(s) 1523, 1701
BsmBI CGTCTC 1 cut(s) 1523
BsmI GAATGC 3 cut(s) 200, 614, 917
BsnI GGCC 5 cut(s) 334, 1052, 1356, 2153, 2217
BsoBI CYCGRG 1 cut(s) 2031
Bsp1286I GDGCHC 4 cut(s) 458, 779, 1663, 1917
Bsp1407I TGTACA 1 cut(s) 1173
Bsp143I GATC 2 cut(s) 1840, 2133
BspACI CCGC 3 cut(s) 114, 338, 779
BspANI GGCC 5 cut(s) 334, 1052, 1356, 2153, 2217
BspCNI CTCAG 3 cut(s) 1031, 1594, 1864
BspLI GGNNCC 2 cut(s) 776, 1842
BspMI ACCTGC 1 cut(s) 1046
BspPI GGATC 2 cut(s) 1835, 1848
BsrDI GCAATG 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 1173
BsrI ACTGG 4 cut(s) 212, 249, 707, 1934
BssECI CCNNGG 1 cut(s) 1357
BssMI GATC 2 cut(s) 1840, 2133
BssNI GRCGYC 4 cut(s) 151, 235, 681, 1884
BssT1I CCWWGG 1 cut(s) 1357
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 8 cut(s) 386, 445, 518, 633, 997, 1375, 2266, 2302
BstACI GRCGYC 4 cut(s) 151, 235, 681, 1884
BstAPI GCANNNNNTGC 1 cut(s) 1757
BstAUI TGTACA 1 cut(s) 1173
BstC8I GCNNGC 5 cut(s) 336, 764, 779, 862, 917
BstDEI CTNAG 4 cut(s) 1018, 1154, 1581, 1872
BstF5I GGATG 6 cut(s) 126, 1402, 1587, 1741, 2154, 2248
BstH2I RGCGCY 1 cut(s) 2119
BstHHI GCGC 4 cut(s) 21, 54, 426, 2118
BstKTI GATC 2 cut(s) 1843, 2136
BstMAI GTCTC 2 cut(s) 1523, 1701
BstMBI GATC 2 cut(s) 1840, 2133
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 5 cut(s) 16, 331, 1757, 1912, 2081
BstNI CCWGG 1 cut(s) 620
BstNSI RCATGY 5 cut(s) 801, 1014, 1390, 1679, 2334
BstPAI GACNNNNGTC 1 cut(s) 2184
BstSCI CCNGG 1 cut(s) 618
BstSFI CTRYAG 1 cut(s) 1888
BstSLI GKGCMC 1 cut(s) 458
BstV1I GCAGC 3 cut(s) 299, 408, 1147
BstV2I GAAGAC 3 cut(s) 648, 1959, 2333
BstX2I RGATCY 1 cut(s) 1840
BstXI CCANNNNNNTGG 1 cut(s) 1620
BstYI RGATCY 1 cut(s) 1840
BsuRI GGCC 5 cut(s) 334, 1052, 1356, 2153, 2217
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 459
BtsCI GGATG 6 cut(s) 126, 1402, 1587, 1741, 2154, 2248
BtsI GCAGTG 2 cut(s) 116, 2243
BtsIMutI CAGTG 7 cut(s) 77, 116, 441, 638, 993, 2243, 2271
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 5 cut(s) 336, 764, 779, 862, 917
CfoI GCGC 4 cut(s) 21, 54, 426, 2118
CseI GACGC 3 cut(s) 224, 547, 2189
DdeI CTNAG 4 cut(s) 1018, 1154, 1581, 1872
DpnI GATC 2 cut(s) 1842, 2135
DpnII GATC 2 cut(s) 1840, 2133
DraI TTTAAA 1 cut(s) 2097
DriI GACNNNNNGTC 1 cut(s) 1714
EaeI YGGCCR 4 cut(s) 332, 1050, 1354, 2215
Eam1105I GACNNNNNGTC 1 cut(s) 1714
Ecl136II GAGCTC 2 cut(s) 1661, 1915
Eco130I CCWWGG 1 cut(s) 1357
Eco24I GRGCYC 3 cut(s) 779, 1663, 1917
Eco53kI GAGCTC 2 cut(s) 1661, 1915
Eco57I CTGAAG 4 cut(s) 101, 266, 1623, 1665
Eco88I CYCGRG 1 cut(s) 2031
EcoICRI GAGCTC 2 cut(s) 1661, 1915
EcoRI GAATTC 2 cut(s) 1454, 1607
EcoRII CCWGG 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 1357
EcoT22I ATGCAT 2 cut(s) 481, 616
EcoT38I GRGCYC 3 cut(s) 779, 1663, 1917
ErhI CCWWGG 1 cut(s) 1357
Esp3I CGTCTC 1 cut(s) 1523
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 1527, 1559
FauI CCCGC 1 cut(s) 786
FauNDI CATATG 1 cut(s) 475
FblI GTMKAC 4 cut(s) 238, 1322, 1434, 1887
Fnu4HI GCNGC 4 cut(s) 313, 339, 422, 1161
FokI GGATG 6 cut(s) 113, 1409, 1574, 1748, 2141, 2255
FriOI GRGCYC 3 cut(s) 779, 1663, 1917
Fsp4HI GCNGC 4 cut(s) 313, 339, 422, 1161
FspBI CTAG 6 cut(s) 11, 698, 1247, 1526, 2256, 2315
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 4 cut(s) 20, 53, 425, 2117
GluI GCNGC 4 cut(s) 313, 339, 422, 1161
GsaI CCCAGC 2 cut(s) 453, 762
GsuI CTGGAG 2 cut(s) 266, 724
HaeII RGCGCY 1 cut(s) 2119
HaeIII GGCC 5 cut(s) 334, 1052, 1356, 2153, 2217
HapII CCGG 4 cut(s) 523, 857, 1495, 1923
HgaI GACGC 3 cut(s) 224, 547, 2189
HhaI GCGC 4 cut(s) 21, 54, 426, 2118
Hin1I GRCGYC 4 cut(s) 151, 235, 681, 1884
Hin6I GCGC 4 cut(s) 19, 52, 424, 2116
HinP1I GCGC 4 cut(s) 19, 52, 424, 2116
HincII GTYRAC 4 cut(s) 462, 625, 2189, 2271
HindII GTYRAC 4 cut(s) 462, 625, 2189, 2271
HinfI GANTC 8 cut(s) 785, 1287, 1627, 1861, 1969, 2006, 2229, 2290
HpaII CCGG 4 cut(s) 523, 857, 1495, 1923
HphI GGTGA 3 cut(s) 66, 1280, 1793
Hpy188I TCNGA 6 cut(s) 348, 1001, 1021, 1453, 2167, 2289
Hpy188III TCNNGA 9 cut(s) 142, 229, 491, 899, 941, 1776, 1844, 1918, 2024
Hpy99I CGWCG 2 cut(s) 686, 2183
HpyAV CCTTC 6 cut(s) 85, 213, 863, 893, 1300, 2039
HpyCH4III ACNGT 8 cut(s) 386, 445, 518, 633, 997, 1375, 2266, 2302
HpyCH4IV ACGT 7 cut(s) 151, 458, 681, 892, 1207, 1815, 1884
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 331, 1757, 1912, 2081
HpyF3I CTNAG 4 cut(s) 1018, 1154, 1581, 1872
HpySE526I ACGT 7 cut(s) 151, 458, 681, 892, 1207, 1815, 1884
Hsp92I GRCGYC 4 cut(s) 151, 235, 681, 1884
HspAI GCGC 4 cut(s) 19, 52, 424, 2116
Kzo9I GATC 2 cut(s) 1840, 2133
LmnI GCTCC 6 cut(s) 94, 705, 774, 1658, 2264, 2290
Lsp1109I GCAGC 3 cut(s) 299, 408, 1147
LweI GCATC 7 cut(s) 74, 113, 135, 684, 967, 2017, 2272
MaeI CTAG 6 cut(s) 11, 698, 1247, 1526, 2256, 2315
MaeII ACGT 7 cut(s) 151, 458, 681, 892, 1207, 1815, 1884
MaeIII GTNAC 8 cut(s) 286, 404, 986, 1268, 1311, 1642, 1880, 1936
MalI GATC 2 cut(s) 1842, 2135
MboI GATC 2 cut(s) 1840, 2133
MboII GAAGA 9 cut(s) 94, 597, 653, 1413, 1616, 1964, 2015, 2121, 2291
MflI RGATCY 1 cut(s) 1840
MhlI GDGCHC 4 cut(s) 458, 779, 1663, 1917
MlsI TGGCCA 3 cut(s) 1052, 1356, 2217
MluNI TGGCCA 3 cut(s) 1052, 1356, 2217
MlyI GAGTC 4 cut(s) 779, 1870, 1978, 2284
MmeI TCCRAC 4 cut(s) 913, 979, 2127, 2312
Mox20I TGGCCA 3 cut(s) 1052, 1356, 2217
Mph1103I ATGCAT 2 cut(s) 481, 616
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 3 cut(s) 1052, 1356, 2217
MseI TTAA 8 cut(s) 381, 512, 710, 734, 972, 1203, 1275, 2096
MslI CAYNNNNRTG 4 cut(s) 109, 796, 1391, 1618
Msp20I TGGCCA 3 cut(s) 1052, 1356, 2217
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 4 cut(s) 523, 857, 1495, 1923
MspR9I CCNGG 1 cut(s) 620
Mva1269I GAATGC 3 cut(s) 200, 614, 917
MvaI CCWGG 1 cut(s) 620
MwoI GCNNNNNNNGC 5 cut(s) 16, 331, 1757, 1912, 2081
NdeI CATATG 1 cut(s) 475
NdeII GATC 2 cut(s) 1840, 2133
NlaIV GGNNCC 2 cut(s) 776, 1842
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 3 cut(s) 404, 1268, 1880
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 2 cut(s) 481, 616
NspI RCATGY 5 cut(s) 801, 1014, 1390, 1679, 2334
PaeR7I CTCGAG 1 cut(s) 2031
PciI ACATGT 1 cut(s) 2330
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 3 cut(s) 200, 614, 917
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 4 cut(s) 1287, 1627, 2006, 2229
PkrI GCNGC 4 cut(s) 314, 340, 423, 1162
PleI GAGTC 4 cut(s) 779, 1869, 1977, 2284
PpsI GAGTC 4 cut(s) 779, 1869, 1977, 2284
PscI ACATGT 1 cut(s) 2330
PshAI GACNNNNGTC 1 cut(s) 2184
PshBI ATTAAT 1 cut(s) 734
Psp124BI GAGCTC 2 cut(s) 1663, 1917
Psp1406I AACGTT 1 cut(s) 892
Psp6I CCWGG 1 cut(s) 618
PspFI CCCAGC 2 cut(s) 449, 758
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 776, 1842
PspXI VCTCGAGB 1 cut(s) 2031
PsuI RGATCY 1 cut(s) 1840
RseI CAYNNNNRTG 4 cut(s) 109, 796, 1391, 1618
SacI GAGCTC 2 cut(s) 1663, 1917
SaqAI TTAA 8 cut(s) 381, 512, 710, 734, 972, 1203, 1275, 2096
SatI GCNGC 4 cut(s) 313, 339, 422, 1161
Sau3AI GATC 2 cut(s) 1840, 2133
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 4 cut(s) 779, 1870, 1978, 2284
ScrFI CCNGG 1 cut(s) 620
SduI GDGCHC 4 cut(s) 458, 779, 1663, 1917
SfaNI GCATC 7 cut(s) 74, 113, 135, 684, 967, 2017, 2272
SfcI CTRYAG 1 cut(s) 1888
Sfr274I CTCGAG 1 cut(s) 2031
SlaI CTCGAG 1 cut(s) 2031
SmiMI CAYNNNNRTG 4 cut(s) 109, 796, 1391, 1618
SmlI CTYRAG 3 cut(s) 229, 1103, 2031
SmoI CTYRAG 3 cut(s) 229, 1103, 2031
SpeI ACTAGT 1 cut(s) 2314
SsiI CCGC 3 cut(s) 114, 338, 779
SspI AATATT 3 cut(s) 259, 952, 970
SspMI CTAG 6 cut(s) 11, 698, 1247, 1526, 2256, 2315
SstI GAGCTC 2 cut(s) 1663, 1917
StyD4I CCNGG 1 cut(s) 618
StyI CCWWGG 1 cut(s) 1357
TaaI ACNGT 8 cut(s) 386, 445, 518, 633, 997, 1375, 2266, 2302
TaiI ACGT 7 cut(s) 154, 461, 684, 895, 1210, 1818, 1887
TaqI TCGA 5 cut(s) 1304, 1859, 2032, 2227, 2232
TaqII GACCGA 1 cut(s) 611
TatI WGTACW 5 cut(s) 59, 366, 467, 1173, 1584
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 4 cut(s) 1287, 1627, 2006, 2229
Tru1I TTAA 8 cut(s) 381, 512, 710, 734, 972, 1203, 1275, 2096
Tru9I TTAA 8 cut(s) 381, 512, 710, 734, 972, 1203, 1275, 2096
TscAI CASTG 7 cut(s) 84, 116, 448, 638, 1000, 2243, 2271
TseFI GTSAC 3 cut(s) 404, 1268, 1880
TseI GCWGC 3 cut(s) 312, 421, 1160
Tsp45I GTSAC 3 cut(s) 404, 1268, 1880
TspDTI ATGAA 7 cut(s) 19, 591, 649, 924, 1447, 1569, 2058
TspGWI ACGGA 4 cut(s) 810, 867, 918, 1547
TspRI CASTG 7 cut(s) 84, 116, 448, 638, 1000, 2243, 2271
VneI GTGCAC 1 cut(s) 454
VspI ATTAAT 1 cut(s) 734
XapI RAATTY 7 cut(s) 655, 1043, 1454, 1556, 1607, 2098, 2210
XceI RCATGY 5 cut(s) 801, 1014, 1390, 1679, 2334
XhoI CTCGAG 1 cut(s) 2031
XmiI GTMKAC 4 cut(s) 238, 1322, 1434, 1887
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 6 cut(s) 11, 698, 1247, 1526, 2256, 2315
ZraI GACGTC 3 cut(s) 152, 682, 1885
ZrmI AGTACT 1 cut(s) 61
Zsp2I ATGCAT 2 cut(s) 481, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.