wall-associated receptor kinase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 98 |
| Acc65I | GGTACC | 1 cut(s) | 833 |
| AccB1I | GGYRCC | 1 cut(s) | 833 |
| AccI | GTMKAC | 1 cut(s) | 744 |
| AciI | CCGC | 1 cut(s) | 490 |
| AclWI | GGATC | 1 cut(s) | 298 |
| AcoI | YGGCCR | 2 cut(s) | 273, 817 |
| AcsI | RAATTY | 4 cut(s) | 810, 916, 1019, 1558 |
| AfaI | GTAC | 5 cut(s) | 88, 835, 1133, 1281, 1747 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1066, 1624, 1701 |
| AgsI | TTSAA | 8 cut(s) | 13, 330, 381, 428, 449, 763, 1455, 1564 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1177 |
| AjnI | CCWGG | 4 cut(s) | 111, 198, 357, 523 |
| AluBI | AGCT | 6 cut(s) | 21, 785, 1124, 1356, 1378, 1634 |
| AluI | AGCT | 6 cut(s) | 21, 785, 1124, 1356, 1378, 1634 |
| Alw21I | GWGCWC | 1 cut(s) | 1380 |
| Alw26I | GTCTC | 3 cut(s) | 121, 986, 1164 |
| AlwI | GGATC | 1 cut(s) | 298 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 90 |
| Ama87I | CYCGRG | 1 cut(s) | 1491 |
| AoxI | GGCC | 4 cut(s) | 273, 573, 817, 826 |
| ApoI | RAATTY | 4 cut(s) | 810, 916, 1019, 1558 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 542, 574 |
| AseI | ATTAAT | 1 cut(s) | 924 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 325, 1039 |
| Asp718I | GGTACC | 1 cut(s) | 833 |
| AspLEI | GCGC | 1 cut(s) | 1095 |
| AspS9I | GGNCC | 1 cut(s) | 1366 |
| AsuHPI | GGTGA | 3 cut(s) | 743, 917, 1049 |
| AsuII | TTCGAA | 1 cut(s) | 767 |
| AvaI | CYCGRG | 1 cut(s) | 1491 |
| AvaII | GGWCC | 1 cut(s) | 1366 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1479, 1512 |
| BalI | TGGCCA | 1 cut(s) | 819 |
| BanI | GGYRCC | 1 cut(s) | 833 |
| BanII | GRGCYC | 1 cut(s) | 1380 |
| BauI | CACGAG | 1 cut(s) | 1275 |
| BbsI | GAAGAC | 1 cut(s) | 1173 |
| Bbv12I | GWGCWC | 1 cut(s) | 1380 |
| BccI | CCATC | 4 cut(s) | 20, 854, 1102, 1499 |
| BceAI | ACGGC | 1 cut(s) | 260 |
| BciT130I | CCWGG | 4 cut(s) | 113, 200, 359, 525 |
| BcoDI | GTCTC | 3 cut(s) | 121, 986, 1164 |
| BfaI | CTAG | 3 cut(s) | 6, 1301, 1433 |
| BfmI | CTRYAG | 2 cut(s) | 561, 1749 |
| BfuAI | ACCTGC | 1 cut(s) | 98 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 825 |
| BglII | AGATCT | 2 cut(s) | 344, 1241 |
| BlpI | GCTNAGC | 1 cut(s) | 1374 |
| BmcAI | AGTACT | 1 cut(s) | 1747 |
| Bme1390I | CCNGG | 4 cut(s) | 113, 200, 359, 525 |
| Bme18I | GGWCC | 1 cut(s) | 1366 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1177 |
| BmeT110I | CYCGRG | 1 cut(s) | 1491 |
| BmgT120I | GGNCC | 1 cut(s) | 1366 |
| BmiI | GGNNCC | 3 cut(s) | 322, 480, 835 |
| BmrFI | CCNGG | 4 cut(s) | 113, 200, 359, 525 |
| BmrI | ACTGGG | 1 cut(s) | 52 |
| BmsI | GCATC | 3 cut(s) | 1541, 1633, 1756 |
| BmuI | ACTGGG | 1 cut(s) | 52 |
| BpiI | GAAGAC | 1 cut(s) | 1173 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1374 |
| Bpu14I | TTCGAA | 1 cut(s) | 767 |
| BpuEI | CTTGAG | 1 cut(s) | 379 |
| BsaAI | YACGTR | 1 cut(s) | 48 |
| BsaI | GGTCTC | 1 cut(s) | 121 |
| BsaJI | CCNNGG | 3 cut(s) | 820, 829, 1695 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 894, 924 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1066, 1624, 1701 |
| Bse118I | RCCGGY | 2 cut(s) | 271, 486 |
| Bse1I | ACTGG | 5 cut(s) | 58, 150, 209, 576, 1323 |
| Bse3DI | GCAATG | 2 cut(s) | 100, 423 |
| BseBI | CCWGG | 4 cut(s) | 113, 200, 359, 525 |
| BseDI | CCNNGG | 3 cut(s) | 820, 829, 1695 |
| BseGI | GGATG | 4 cut(s) | 458, 520, 1204, 1711 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1066, 1624, 1701 |
| BseMI | GCAATG | 2 cut(s) | 100, 423 |
| BseMII | CTCAG | 5 cut(s) | 36, 1058, 1365, 1428, 1674 |
| BseNI | ACTGG | 5 cut(s) | 58, 150, 209, 576, 1323 |
| BseRI | GAGGAG | 1 cut(s) | 1082 |
| BseX3I | CGGCCG | 1 cut(s) | 273 |
| Bsh1285I | CGRYCG | 1 cut(s) | 276 |
| BshFI | GGCC | 4 cut(s) | 275, 575, 819, 828 |
| BshNI | GGYRCC | 1 cut(s) | 833 |
| BsiEI | CGRYCG | 1 cut(s) | 276 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1380 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1491 |
| BsiSI | CCGG | 2 cut(s) | 272, 487 |
| BslFI | GGGAC | 1 cut(s) | 40 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1066, 1624, 1701 |
| BsmAI | GTCTC | 3 cut(s) | 121, 986, 1164 |
| BsmBI | CGTCTC | 1 cut(s) | 986 |
| BsmFI | GGGAC | 1 cut(s) | 40 |
| BsmI | GAATGC | 4 cut(s) | 185, 476, 542, 855 |
| BsnI | GGCC | 4 cut(s) | 275, 575, 819, 828 |
| Bso31I | GGTCTC | 1 cut(s) | 121 |
| BsoBI | CYCGRG | 1 cut(s) | 1491 |
| Bsp119I | TTCGAA | 1 cut(s) | 767 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1380 |
| Bsp143I | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1374 |
| BspACI | CCGC | 1 cut(s) | 490 |
| BspANI | GGCC | 4 cut(s) | 275, 575, 819, 828 |
| BspCNI | CTCAG | 5 cut(s) | 35, 1057, 1366, 1427, 1673 |
| BspLI | GGNNCC | 3 cut(s) | 322, 480, 835 |
| BspMAI | CTGCAG | 1 cut(s) | 565 |
| BspMI | ACCTGC | 1 cut(s) | 98 |
| BspPI | GGATC | 1 cut(s) | 298 |
| BspT104I | TTCGAA | 1 cut(s) | 767 |
| BspT107I | GGYRCC | 1 cut(s) | 833 |
| BspTNI | GGTCTC | 1 cut(s) | 121 |
| BsrDI | GCAATG | 2 cut(s) | 100, 423 |
| BsrFI | RCCGGY | 2 cut(s) | 271, 486 |
| BsrI | ACTGG | 5 cut(s) | 58, 150, 209, 576, 1323 |
| BssAI | RCCGGY | 2 cut(s) | 271, 486 |
| BssECI | CCNNGG | 3 cut(s) | 820, 829, 1695 |
| BssMI | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| BssSI | CACGAG | 1 cut(s) | 1275 |
| BssT1I | CCWWGG | 2 cut(s) | 820, 829 |
| Bst2BI | CACGAG | 1 cut(s) | 1275 |
| Bst2UI | CCWGG | 4 cut(s) | 113, 200, 359, 525 |
| Bst4CI | ACNGT | 2 cut(s) | 609, 838 |
| Bst6I | CTCTTC | 2 cut(s) | 331, 382 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 90 |
| BstBAI | YACGTR | 1 cut(s) | 48 |
| BstBI | TTCGAA | 1 cut(s) | 767 |
| BstC8I | GCNNGC | 2 cut(s) | 82, 273 |
| BstDEI | CTNAG | 5 cut(s) | 22, 1044, 1374, 1414, 1660 |
| BstF5I | GGATG | 4 cut(s) | 458, 520, 1204, 1711 |
| BstHHI | GCGC | 1 cut(s) | 1095 |
| BstKTI | GATC | 4 cut(s) | 306, 347, 1244, 1678 |
| BstMAI | GTCTC | 3 cut(s) | 121, 986, 1164 |
| BstMBI | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| BstMCI | CGRYCG | 1 cut(s) | 276 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 27, 77, 90, 191, 825, 1220, 1541 |
| BstNI | CCWGG | 4 cut(s) | 113, 200, 359, 525 |
| BstSCI | CCNGG | 4 cut(s) | 111, 198, 357, 523 |
| BstSFI | CTRYAG | 2 cut(s) | 561, 1749 |
| BstV2I | GAAGAC | 1 cut(s) | 1173 |
| BstX2I | RGATCY | 2 cut(s) | 344, 1241 |
| BstYI | RGATCY | 2 cut(s) | 344, 1241 |
| BstZI | CGGCCG | 1 cut(s) | 273 |
| BsuRI | GGCC | 4 cut(s) | 275, 575, 819, 828 |
| BtsCI | GGATG | 4 cut(s) | 458, 520, 1204, 1711 |
| BtsIMutI | CAGTG | 2 cut(s) | 65, 1332 |
| BveI | ACCTGC | 1 cut(s) | 98 |
| Cac8I | GCNNGC | 2 cut(s) | 82, 273 |
| CaiI | CAGNNNCTG | 1 cut(s) | 90 |
| CfoI | GCGC | 1 cut(s) | 1095 |
| Cfr10I | RCCGGY | 2 cut(s) | 271, 486 |
| Cfr13I | GGNCC | 1 cut(s) | 1366 |
| Csp6I | GTAC | 5 cut(s) | 87, 834, 1132, 1280, 1746 |
| CviAII | CATG | 3 cut(s) | 174, 1153, 1734 |
| CviQI | GTAC | 5 cut(s) | 87, 834, 1132, 1280, 1746 |
| DdeI | CTNAG | 5 cut(s) | 22, 1044, 1374, 1414, 1660 |
| DpnI | GATC | 4 cut(s) | 305, 346, 1243, 1677 |
| DpnII | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| DraI | TTTAAA | 1 cut(s) | 688 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1177 |
| EaeI | YGGCCR | 2 cut(s) | 273, 817 |
| EagI | CGGCCG | 1 cut(s) | 273 |
| Eam1104I | CTCTTC | 2 cut(s) | 331, 382 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1177 |
| EarI | CTCTTC | 2 cut(s) | 331, 382 |
| Ecl136II | GAGCTC | 1 cut(s) | 1378 |
| EclXI | CGGCCG | 1 cut(s) | 273 |
| Eco130I | CCWWGG | 2 cut(s) | 820, 829 |
| Eco24I | GRGCYC | 1 cut(s) | 1380 |
| Eco31I | GGTCTC | 1 cut(s) | 121 |
| Eco47I | GGWCC | 1 cut(s) | 1366 |
| Eco52I | CGGCCG | 1 cut(s) | 273 |
| Eco53kI | GAGCTC | 1 cut(s) | 1378 |
| Eco88I | CYCGRG | 1 cut(s) | 1491 |
| EcoICRI | GAGCTC | 1 cut(s) | 1378 |
| EcoRI | GAATTC | 2 cut(s) | 810, 1019 |
| EcoRII | CCWGG | 4 cut(s) | 111, 198, 357, 523 |
| EcoT14I | CCWWGG | 2 cut(s) | 820, 829 |
| EcoT22I | ATGCAT | 1 cut(s) | 187 |
| EcoT38I | GRGCYC | 1 cut(s) | 1380 |
| ErhI | CCWWGG | 2 cut(s) | 820, 829 |
| Esp3I | CGTCTC | 1 cut(s) | 986 |
| FaeI | CATG | 3 cut(s) | 177, 1156, 1737 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 990, 1022 |
| FaqI | GGGAC | 1 cut(s) | 40 |
| FatI | CATG | 3 cut(s) | 173, 1152, 1733 |
| FblI | GTMKAC | 1 cut(s) | 744 |
| FokI | GGATG | 4 cut(s) | 465, 507, 1211, 1718 |
| FriOI | GRGCYC | 1 cut(s) | 1380 |
| FspBI | CTAG | 3 cut(s) | 6, 1301, 1433 |
| GlaI | GCGC | 1 cut(s) | 1094 |
| HaeIII | GGCC | 4 cut(s) | 275, 575, 819, 828 |
| HapII | CCGG | 2 cut(s) | 272, 487 |
| HhaI | GCGC | 1 cut(s) | 1095 |
| Hin1II | CATG | 3 cut(s) | 177, 1156, 1737 |
| Hin6I | GCGC | 1 cut(s) | 1093 |
| HinP1I | GCGC | 1 cut(s) | 1093 |
| HincII | GTYRAC | 2 cut(s) | 352, 745 |
| HindII | GTYRAC | 2 cut(s) | 352, 745 |
| HinfI | GANTC | 2 cut(s) | 517, 1614 |
| HpaI | GTTAAC | 1 cut(s) | 352 |
| HpaII | CCGG | 2 cut(s) | 272, 487 |
| HphI | GGTGA | 3 cut(s) | 743, 917, 1049 |
| Hpy166II | GTNNAC | 6 cut(s) | 219, 352, 502, 745, 805, 841 |
| Hpy188I | TCNGA | 6 cut(s) | 25, 340, 1407, 1417, 1642, 1663 |
| Hpy188III | TCNNGA | 6 cut(s) | 458, 1239, 1301, 1381, 1484, 1511 |
| Hpy8I | GTNNAC | 6 cut(s) | 219, 352, 502, 745, 805, 841 |
| HpyAV | CCTTC | 7 cut(s) | 135, 151, 422, 443, 667, 763, 1454 |
| HpyCH4III | ACNGT | 2 cut(s) | 609, 838 |
| HpyCH4IV | ACGT | 1 cut(s) | 47 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 27, 77, 90, 191, 825, 1220, 1541 |
| HpyF3I | CTNAG | 5 cut(s) | 22, 1044, 1374, 1414, 1660 |
| HpySE526I | ACGT | 1 cut(s) | 47 |
| Hsp92II | CATG | 3 cut(s) | 177, 1156, 1737 |
| HspAI | GCGC | 1 cut(s) | 1093 |
| KpnI | GGTACC | 1 cut(s) | 837 |
| KroI | GCCGGC | 1 cut(s) | 271 |
| KroNI | GCCGGC | 1 cut(s) | 273 |
| KspAI | GTTAAC | 1 cut(s) | 352 |
| Kzo9I | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| LmnI | GCTCC | 3 cut(s) | 484, 712, 1121 |
| LweI | GCATC | 3 cut(s) | 1541, 1633, 1756 |
| MaeI | CTAG | 3 cut(s) | 6, 1301, 1433 |
| MaeII | ACGT | 1 cut(s) | 47 |
| MalI | GATC | 4 cut(s) | 305, 346, 1243, 1677 |
| MboI | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| MboII | GAAGA | 9 cut(s) | 318, 369, 670, 869, 876, 1173, 1526, 1529, 1749 |
| MfeI | CAATTG | 1 cut(s) | 1619 |
| MflI | RGATCY | 2 cut(s) | 344, 1241 |
| MhlI | GDGCHC | 1 cut(s) | 1380 |
| MlsI | TGGCCA | 1 cut(s) | 819 |
| MluNI | TGGCCA | 1 cut(s) | 819 |
| MmeI | TCCRAC | 5 cut(s) | 261, 690, 880, 892, 1587 |
| Mox20I | TGGCCA | 1 cut(s) | 819 |
| Mph1103I | ATGCAT | 1 cut(s) | 187 |
| MroNI | GCCGGC | 1 cut(s) | 271 |
| MroXI | GAANNNNTTC | 2 cut(s) | 325, 1039 |
| MscI | TGGCCA | 1 cut(s) | 819 |
| MslI | CAYNNNNRTG | 4 cut(s) | 798, 960, 1062, 1641 |
| Msp20I | TGGCCA | 1 cut(s) | 819 |
| MspI | CCGG | 2 cut(s) | 272, 487 |
| MspR9I | CCNGG | 4 cut(s) | 113, 200, 359, 525 |
| MunI | CAATTG | 1 cut(s) | 1619 |
| Mva1269I | GAATGC | 4 cut(s) | 185, 476, 542, 855 |
| MvaI | CCWGG | 4 cut(s) | 113, 200, 359, 525 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 27, 77, 90, 191, 825, 1220, 1541 |
| NaeI | GCCGGC | 1 cut(s) | 273 |
| NdeII | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| NgoMIV | GCCGGC | 1 cut(s) | 271 |
| NlaIII | CATG | 3 cut(s) | 177, 1156, 1737 |
| NlaIV | GGNNCC | 3 cut(s) | 322, 480, 835 |
| NsiI | ATGCAT | 1 cut(s) | 187 |
| NspV | TTCGAA | 1 cut(s) | 767 |
| PaeR7I | CTCGAG | 1 cut(s) | 1491 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1482 |
| PctI | GAATGC | 4 cut(s) | 185, 476, 542, 855 |
| PdiI | GCCGGC | 1 cut(s) | 273 |
| PdmI | GAANNNNTTC | 2 cut(s) | 325, 1039 |
| PfeI | GAWTC | 2 cut(s) | 517, 1614 |
| PfoI | TCCNGGA | 1 cut(s) | 523 |
| Ppu21I | YACGTR | 1 cut(s) | 48 |
| PshBI | ATTAAT | 1 cut(s) | 924 |
| Psp124BI | GAGCTC | 1 cut(s) | 1380 |
| Psp6I | CCWGG | 4 cut(s) | 111, 198, 357, 523 |
| PspGI | CCWGG | 4 cut(s) | 111, 198, 357, 523 |
| PspN4I | GGNNCC | 3 cut(s) | 322, 480, 835 |
| PspPI | GGNCC | 1 cut(s) | 1366 |
| PspXI | VCTCGAGB | 1 cut(s) | 1491 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1738, 1770 |
| PstI | CTGCAG | 1 cut(s) | 565 |
| PstNI | CAGNNNCTG | 1 cut(s) | 90 |
| PsuI | RGATCY | 2 cut(s) | 344, 1241 |
| RsaI | GTAC | 5 cut(s) | 88, 835, 1133, 1281, 1747 |
| RsaNI | GTAC | 5 cut(s) | 87, 834, 1132, 1280, 1746 |
| RseI | CAYNNNNRTG | 4 cut(s) | 798, 960, 1062, 1641 |
| SacI | GAGCTC | 1 cut(s) | 1380 |
| SalI | GTCGAC | 1 cut(s) | 743 |
| Sau3AI | GATC | 4 cut(s) | 303, 344, 1241, 1675 |
| Sau96I | GGNCC | 1 cut(s) | 1366 |
| ScaI | AGTACT | 1 cut(s) | 1747 |
| ScrFI | CCNGG | 4 cut(s) | 113, 200, 359, 525 |
| SduI | GDGCHC | 1 cut(s) | 1380 |
| SfaNI | GCATC | 3 cut(s) | 1541, 1633, 1756 |
| SfcI | CTRYAG | 2 cut(s) | 561, 1749 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 825 |
| Sfr274I | CTCGAG | 1 cut(s) | 1491 |
| SfuI | TTCGAA | 1 cut(s) | 767 |
| SinI | GGWCC | 1 cut(s) | 1366 |
| SlaI | CTCGAG | 1 cut(s) | 1491 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 798, 960, 1062, 1641 |
| SmlI | CTYRAG | 2 cut(s) | 394, 1491 |
| SmoI | CTYRAG | 2 cut(s) | 394, 1491 |
| SsiI | CCGC | 1 cut(s) | 490 |
| SspI | AATATT | 1 cut(s) | 1313 |
| SspMI | CTAG | 3 cut(s) | 6, 1301, 1433 |
| SstI | GAGCTC | 1 cut(s) | 1380 |
| StyD4I | CCNGG | 4 cut(s) | 111, 198, 357, 523 |
| StyI | CCWWGG | 2 cut(s) | 820, 829 |
| TaaI | ACNGT | 2 cut(s) | 609, 838 |
| TaiI | ACGT | 1 cut(s) | 50 |
| TaqII | GACCGA | 1 cut(s) | 1709 |
| TatI | WGTACW | 2 cut(s) | 1279, 1745 |
| TfiI | GAWTC | 2 cut(s) | 517, 1614 |
| TscAI | CASTG | 2 cut(s) | 65, 1339 |
| TspGWI | ACGGA | 1 cut(s) | 1010 |
| TspRI | CASTG | 2 cut(s) | 65, 1339 |
| VpaK11BI | GGWCC | 1 cut(s) | 1366 |
| VspI | ATTAAT | 1 cut(s) | 924 |
| XapI | RAATTY | 4 cut(s) | 810, 916, 1019, 1558 |
| XbaI | TCTAGA | 1 cut(s) | 1300 |
| XhoI | CTCGAG | 1 cut(s) | 1491 |
| XmiI | GTMKAC | 1 cut(s) | 744 |
| XmnI | GAANNNNTTC | 2 cut(s) | 325, 1039 |
| XspI | CTAG | 3 cut(s) | 6, 1301, 1433 |
| ZrmI | AGTACT | 1 cut(s) | 1747 |
| Zsp2I | ATGCAT | 1 cut(s) | 187 |