Rroxscaffold_2G00095450

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000052
Physical Location & Seq
Forward (+)
16803564 .. 16806265
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095450.1

Sequence Viewer

Length: 1884 bp
ATGCAATCCCTAGTTCATCATATGATATCTATTATAGTTTTATGTTTATGGAGCTTTACTACAGCAGCAGTAGCAGCAGAATCAGGAGCAGCACTGCCGATAGCAAAGCCGAATTGCCAAGAGAAATGCGGAAATGTCAGCCTCCCGTACCCTTTCGGGATCGGGCCTGATAAAGATTGTTACCTTGAAGAGTGGTTTGAAATAGACTGCAACCAGTCTACTAGGCACAAGCCTTTCTTGAGGCGTACACAACTGGAGGTGCTGAGCATTTCTATCAAAGACGGCACGCTTCAGGTGAACAGCCCCGTTACTTTCTTCTGCAACGTAACAGGAAGTAGCCAGCCCGCAGATCTGACTGGAAGCCCTTTCGTGTACTCTCAGAGACAAAACAGATTCACAGCAGCGACTTGCGGCTTTGTCTCCTTGGTGAGCTCAGATCAATCGGTGGTTGGAGGGTGCAGGTCAATTTGTGATAAGCAGAATACCGATAGATACTCTGATGGCTGTGATATTGGTACTAATTGTTGCCAGACGAGTATTCCTCCATATCTCAGGGTTGTCACTGCTAGCGTAATGGTGGATGGACCAGCCAACATGACCGGCCGAAATGACAGTGGAAAGCCGGATTGCAGTGACTATGCATTCCTGGTTGATAAAGATTGGTTTGAGACCGCATCAAATTATAGCGCCGTTAGGAATATGCTCCATGTTCCTGTGGCACTAGAATGGAGTGTAATCAATGACACTAGTTCGTCGTTTGCATTTCAAGGAAATAAGAAATGCGATTTTGATACTTCTTTGGCATTTAATCAGTCAGTGCTTTCTTGCCGTTGCCCACAAGGGTTCGATGGAAATCCCTATCTTCTCCAACCTTGTCAAGACATTGATGAGTGCATAGATGGTACTAAAAGATGCTGGCCCGGCAGCAAGTGCATGAACCTTCCGGGTTCGTTCAAATGCGAGGACGAAAAGGATACAATTAAATTGGCACTTGCAGGTCTTGGCTCGAGTCTTGGACTGTTATTATTACTTATCGCTGCATGGTGGGTACACAAACTGGTGAAGAAAAGGAAAAGCATTAAACGCAAGGAAATGTCATTCAAAAGAAACGGAGGTTTGTTGTTAGAAAGACAGTTATCATCTGGTGAAGTTAATCTTGAGAAAATTAAGTTGTTCAAATCAAAAGAGTTGGAGAAGTCCACTGATAATTTTCATGTTGATAGAATCCTTGGCAAAGGTGGCCAAGGGACTGTCTACAAAGGTATGCTAACAGATGGAAGAATCGTTGCAGTAAAAAGGTCTACAAAAGTGGACGAAGGTAGACTTTCACAGTTTATAAATGAGGTTGTTATTCTTTCACAAATCAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACAGAGGTTCCTCTTTTGGTCTATGAGTTTATTCCGAATGGATCCCTTTCCCAATATATTCATGAGCAGCACGAGGATTTTCCACTTACGTGGGAATTGCGCTTAAGGATTGCCACGGAAATTGCAGGAGCTCTTTCCTACTTACACGGTGCAGCTTCAATTCCCATATTTCATAGAGACATCAAGTCGACAAATATATTGCTAGATGAAAAATACAGAGCAAAAATTGCCGACTTTGGAACTTCTAGATCAGTTGCCATAGACCAAACTCACTTGACCACACTTGTATATGGGACATTTGGTTACTTGGACCCAGAATACTTCCAATCGAGCCAATTTACGGATAAAAGTGATGTGTATAGCTTCGGAGTAGTTCTTGTTGAACTCTTGAGTGGACAAAAAACCAATTTCTGTGACAAGATCACAAGAAGAAGGTCGGAGTCTTGCAACACATTTCATTATTTTGATGCAGGATGA

Protein Analysis

627

Amino Acids

69.75

Weight (kDa)

6.67

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 100 5.2e-13 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 294 - 321 2.2e-06 Calcium-binding EGF domain
Pkinase PF00069 404 - 604 7.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 405 - 606 2.9e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1337
Acc36I ACCTGC 2 cut(s) 450, 986
AccI GTMKAC 5 cut(s) 218, 1254, 1301, 1321, 1595
AciI CCGC 4 cut(s) 129, 345, 411, 672
AclWI GGATC 3 cut(s) 167, 1443, 1456
AcoI YGGCCR 2 cut(s) 601, 1240
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 6 cut(s) 149, 247, 374, 517, 904, 1050
AfiI CCNNNNNNNGG 1 cut(s) 1747
AflII CTTAAG 1 cut(s) 1510
AgsI TTSAA 8 cut(s) 188, 200, 767, 955, 1102, 1177, 1566, 1790
AhdI GACNNNNNGTC 1 cut(s) 1591
AhlI ACTAGT 1 cut(s) 746
AjnI CCWGG 1 cut(s) 645
AleI CACNNNNGTG 1 cut(s) 1495
AluBI AGCT 5 cut(s) 54, 432, 1538, 1562, 1770
AluI AGCT 5 cut(s) 54, 432, 1538, 1562, 1770
Alw21I GWGCWC 2 cut(s) 434, 1540
Alw26I GTCTC 5 cut(s) 376, 424, 662, 1400, 1578
AlwI GGATC 3 cut(s) 167, 1443, 1456
Ama87I CYCGRG 1 cut(s) 1006
AoxI GGCC 4 cut(s) 164, 601, 917, 1240
ApeKI GCWGC 8 cut(s) 65, 74, 89, 401, 924, 1037, 1474, 1559
AspLEI GCGC 2 cut(s) 689, 1509
AspS9I GGNCC 4 cut(s) 164, 584, 918, 1717
AsuC2I CCSGG 2 cut(s) 921, 945
AsuHPI GGTGA 4 cut(s) 307, 439, 1072, 1157
AsuNHI GCTAGC 1 cut(s) 566
AvaI CYCGRG 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 584, 1717
BalI TGGCCA 1 cut(s) 1242
BamHI GGATCC 1 cut(s) 1448
BanII GRGCYC 2 cut(s) 434, 1540
BauI CACGAG 1 cut(s) 1478
Bbv12I GWGCWC 2 cut(s) 434, 1540
BbvI GCAGC 8 cut(s) 77, 86, 101, 413, 936, 1024, 1486, 1571
BccI CCATC 5 cut(s) 494, 575, 842, 893, 1268
BceAI ACGGC 3 cut(s) 298, 674, 813
BciT130I CCWGG 1 cut(s) 647
BciVI GTATCC 1 cut(s) 967
BcnI CCSGG 2 cut(s) 921, 945
BcoDI GTCTC 5 cut(s) 376, 424, 662, 1400, 1578
BcuI ACTAGT 1 cut(s) 746
BfaI CTAG 7 cut(s) 11, 222, 567, 722, 747, 1610, 1653
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 690
BfrI CTTAAG 1 cut(s) 1510
BfuAI ACCTGC 2 cut(s) 450, 986
BfuI GTATCC 1 cut(s) 967
BglII AGATCT 1 cut(s) 349
BisI GCNGC 9 cut(s) 66, 75, 90, 402, 412, 925, 1038, 1475, 1560
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 9 cut(s) 67, 76, 91, 403, 413, 926, 1039, 1476, 1561
Bme1390I CCNGG 3 cut(s) 647, 921, 945
Bme18I GGWCC 2 cut(s) 584, 1717
BmeRI GACNNNNNGTC 1 cut(s) 1591
BmeT110I CYCGRG 1 cut(s) 1006
BmgT120I GGNCC 4 cut(s) 164, 584, 918, 1717
BmiI GGNNCC 3 cut(s) 1416, 1450, 1719
BmrFI CCNGG 3 cut(s) 647, 921, 945
BmsI GCATC 3 cut(s) 683, 902, 1864
BmtI GCTAGC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 526, 558
BpmI CTGGAG 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 3 cut(s) 259, 1178, 1816
BpuMI CCSGG 2 cut(s) 921, 945
BsaAI YACGTR 1 cut(s) 1497
BsaBI GATNNNNATC 1 cut(s) 852
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 423, 1228, 1243, 1521
Bsc4I CCNNNNNNNGG 1 cut(s) 1747
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 4 cut(s) 214, 258, 361, 1062
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 423, 1228, 1243, 1521
BseGI GGATG 1 cut(s) 586
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 1 cut(s) 1747
BseMII CTCAG 4 cut(s) 254, 392, 447, 565
BseNI ACTGG 4 cut(s) 214, 258, 361, 1062
BseX3I CGGCCG 1 cut(s) 601
BseXI GCAGC 8 cut(s) 77, 86, 101, 413, 936, 1024, 1486, 1571
BsgI GTGCAG 2 cut(s) 478, 1578
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 4 cut(s) 166, 603, 919, 1242
BsiEI CGRYCG 1 cut(s) 604
BsiHKAI GWGCWC 2 cut(s) 434, 1540
BsiHKCI CYCGRG 1 cut(s) 1006
BsiSI CCGG 4 cut(s) 600, 623, 921, 944
BslFI GGGAC 2 cut(s) 1261, 1714
BslI CCNNNNNNNGG 1 cut(s) 1747
BsmAI GTCTC 5 cut(s) 376, 424, 662, 1400, 1578
BsmFI GGGAC 2 cut(s) 1261, 1714
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 4 cut(s) 166, 603, 919, 1242
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 1 cut(s) 1006
Bsp1286I GDGCHC 2 cut(s) 434, 1540
Bsp143I GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 4 cut(s) 129, 345, 411, 672
BspANI GGCC 4 cut(s) 166, 603, 919, 1242
BspCNI CTCAG 4 cut(s) 255, 391, 446, 564
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 3 cut(s) 1416, 1450, 1719
BspMI ACCTGC 2 cut(s) 450, 986
BspOI GCTAGC 1 cut(s) 570
BspPI GGATC 3 cut(s) 167, 1443, 1456
BspTI CTTAAG 1 cut(s) 1510
BspTNI GGTCTC 1 cut(s) 662
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 4 cut(s) 214, 258, 361, 1062
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 4 cut(s) 423, 1228, 1243, 1521
BssMI GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
BssSI CACGAG 1 cut(s) 1478
BssT1I CCWWGG 3 cut(s) 423, 1228, 1243
Bst2BI CACGAG 1 cut(s) 1478
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 6 cut(s) 614, 1020, 1134, 1252, 1332, 1556
Bst6I CTCTTC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 1510
BstAPI GCANNNNNTGC 2 cut(s) 930, 1634
BstBAI YACGTR 1 cut(s) 1497
BstC8I GCNNGC 5 cut(s) 287, 341, 345, 568, 917
BstDEI CTNAG 4 cut(s) 263, 378, 433, 551
BstDSI CCRYGG 1 cut(s) 1521
BstF5I GGATG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 2 cut(s) 689, 1509
BstKTI GATC 6 cut(s) 162, 352, 439, 1451, 1658, 1830
BstMAI GTCTC 5 cut(s) 376, 424, 662, 1400, 1578
BstMBI GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 7 cut(s) 71, 74, 921, 930, 1083, 1239, 1634
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 3 cut(s) 645, 919, 943
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 8 cut(s) 77, 86, 101, 413, 936, 1024, 1486, 1571
BstX2I RGATCY 2 cut(s) 349, 1448
BstXI CCANNNNNNTGG 1 cut(s) 1497
BstYI RGATCY 2 cut(s) 349, 1448
BstZI CGGCCG 1 cut(s) 601
BsuI GTATCC 1 cut(s) 967
BsuRI GGCC 4 cut(s) 166, 603, 919, 1242
BtgI CCRYGG 1 cut(s) 1521
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 3 cut(s) 92, 561, 637
BtsIMutI CAGTG 6 cut(s) 92, 561, 619, 637, 822, 1200
BveI ACCTGC 2 cut(s) 450, 986
Cac8I GCNNGC 5 cut(s) 287, 341, 345, 568, 917
CciI TCATGA 1 cut(s) 1468
CfoI GCGC 2 cut(s) 689, 1509
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 4 cut(s) 164, 584, 918, 1717
Csp6I GTAC 6 cut(s) 148, 246, 373, 516, 903, 1049
CspCI CAANNNNNGTGG 2 cut(s) 1510, 1545
CviAII CATG 6 cut(s) 595, 707, 934, 1041, 1214, 1469
CviQI GTAC 6 cut(s) 148, 246, 373, 516, 903, 1049
DdeI CTNAG 4 cut(s) 263, 378, 433, 551
DpnI GATC 6 cut(s) 161, 351, 438, 1450, 1657, 1829
DpnII GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
DriI GACNNNNNGTC 1 cut(s) 1591
EaeI YGGCCR 2 cut(s) 601, 1240
EagI CGGCCG 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 183
Eam1105I GACNNNNNGTC 1 cut(s) 1591
EarI CTCTTC 1 cut(s) 183
Ecl136II GAGCTC 2 cut(s) 432, 1538
EclXI CGGCCG 1 cut(s) 601
Eco130I CCWWGG 3 cut(s) 423, 1228, 1243
Eco24I GRGCYC 2 cut(s) 434, 1540
Eco31I GGTCTC 1 cut(s) 662
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 584, 1717
Eco52I CGGCCG 1 cut(s) 601
Eco53kI GAGCTC 2 cut(s) 432, 1538
Eco57I CTGAAG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 1006
EcoICRI GAGCTC 2 cut(s) 432, 1538
EcoRII CCWGG 1 cut(s) 645
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 3 cut(s) 423, 1228, 1243
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 434, 1540
ErhI CCWWGG 3 cut(s) 423, 1228, 1243
FaeI CATG 6 cut(s) 598, 710, 937, 1044, 1217, 1472
FalI AAGNNNNNCTT 6 cut(s) 221, 253, 1140, 1172, 1308, 1340
FaqI GGGAC 2 cut(s) 1261, 1714
FatI CATG 6 cut(s) 594, 706, 933, 1040, 1213, 1468
FauI CCCGC 1 cut(s) 352
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 5 cut(s) 218, 1254, 1301, 1321, 1595
Fnu4HI GCNGC 9 cut(s) 66, 75, 90, 402, 412, 925, 1038, 1475, 1560
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 2 cut(s) 434, 1540
Fsp4HI GCNGC 9 cut(s) 66, 75, 90, 402, 412, 925, 1038, 1475, 1560
FspBI CTAG 7 cut(s) 11, 222, 567, 722, 747, 1610, 1653
GlaI GCGC 2 cut(s) 688, 1508
GluI GCNGC 9 cut(s) 66, 75, 90, 402, 412, 925, 1038, 1475, 1560
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 4 cut(s) 166, 603, 919, 1242
HapII CCGG 4 cut(s) 600, 623, 921, 944
HhaI GCGC 2 cut(s) 689, 1509
Hin1II CATG 6 cut(s) 598, 710, 937, 1044, 1217, 1472
Hin6I GCGC 2 cut(s) 687, 1507
HinP1I GCGC 2 cut(s) 687, 1507
HincII GTYRAC 1 cut(s) 1596
HindII GTYRAC 1 cut(s) 1596
HinfI GANTC 6 cut(s) 80, 393, 1009, 1224, 1281, 1847
HpaII CCGG 4 cut(s) 600, 623, 921, 944
HphI GGTGA 4 cut(s) 307, 439, 1072, 1157
Hpy188I TCNGA 7 cut(s) 354, 381, 436, 499, 1443, 1775, 1846
Hpy188III TCNNGA 8 cut(s) 84, 157, 238, 878, 1157, 1469, 1653, 1795
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 3 cut(s) 950, 1310, 1833
HpyCH4III ACNGT 6 cut(s) 614, 1020, 1134, 1252, 1332, 1556
HpyCH4IV ACGT 2 cut(s) 324, 1496
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 74, 921, 930, 1083, 1239, 1634
HpyF3I CTNAG 4 cut(s) 263, 378, 433, 551
HpySE526I ACGT 2 cut(s) 324, 1496
Hsp92II CATG 6 cut(s) 598, 710, 937, 1044, 1217, 1472
HspAI GCGC 2 cut(s) 687, 1507
Kzo9I GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
LmnI GCTCC 4 cut(s) 51, 86, 708, 1535
Lsp1109I GCAGC 8 cut(s) 77, 86, 101, 413, 936, 1024, 1486, 1571
LweI GCATC 3 cut(s) 683, 902, 1864
MaeI CTAG 7 cut(s) 11, 222, 567, 722, 747, 1610, 1653
MaeII ACGT 2 cut(s) 324, 1496
MaeIII GTNAC 7 cut(s) 179, 307, 325, 559, 632, 1709, 1820
MalI GATC 6 cut(s) 161, 351, 438, 1450, 1657, 1829
MboI GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
MboII GAAGA 6 cut(s) 200, 307, 854, 1075, 1290, 1848
MflI RGATCY 2 cut(s) 349, 1448
MhlI GDGCHC 2 cut(s) 434, 1540
MlsI TGGCCA 1 cut(s) 1242
MluNI TGGCCA 1 cut(s) 1242
MlyI GAGTC 2 cut(s) 1018, 1856
MmeI TCCRAC 4 cut(s) 430, 892, 1170, 1824
Mox20I TGGCCA 1 cut(s) 1242
Mph1103I ATGCAT 1 cut(s) 643
MscI TGGCCA 1 cut(s) 1242
MseI TTAA 7 cut(s) 807, 981, 1080, 1152, 1167, 1383, 1511
MslI CAYNNNNRTG 3 cut(s) 724, 1374, 1495
Msp20I TGGCCA 1 cut(s) 1242
MspCI CTTAAG 1 cut(s) 1510
MspI CCGG 4 cut(s) 600, 623, 921, 944
MspR9I CCNGG 3 cut(s) 647, 921, 945
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 7 cut(s) 71, 74, 921, 930, 1083, 1239, 1634
NciI CCSGG 2 cut(s) 921, 945
NdeI CATATG 1 cut(s) 21
NdeII GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
NheI GCTAGC 1 cut(s) 566
NlaIII CATG 6 cut(s) 598, 710, 937, 1044, 1217, 1472
NlaIV GGNNCC 3 cut(s) 1416, 1450, 1719
NmuCI GTSAC 3 cut(s) 559, 632, 1820
NsiI ATGCAT 1 cut(s) 643
OliI CACNNNNGTG 1 cut(s) 1495
PaeR7I CTCGAG 1 cut(s) 1006
PagI TCATGA 1 cut(s) 1468
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 4 cut(s) 80, 393, 1224, 1281
PkrI GCNGC 9 cut(s) 67, 76, 91, 403, 413, 926, 1039, 1476, 1561
PleI GAGTC 2 cut(s) 1017, 1855
PpsI GAGTC 2 cut(s) 1017, 1855
Ppu21I YACGTR 1 cut(s) 1497
PsiI TTATAA 1 cut(s) 1337
Psp124BI GAGCTC 2 cut(s) 434, 1540
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 3 cut(s) 1416, 1450, 1719
PspPI GGNCC 4 cut(s) 164, 584, 918, 1717
PspXI VCTCGAGB 1 cut(s) 1006
PsuI RGATCY 2 cut(s) 349, 1448
RsaI GTAC 6 cut(s) 149, 247, 374, 517, 904, 1050
RsaNI GTAC 6 cut(s) 148, 246, 373, 516, 903, 1049
RseI CAYNNNNRTG 3 cut(s) 724, 1374, 1495
SacI GAGCTC 2 cut(s) 434, 1540
SalI GTCGAC 1 cut(s) 1594
SaqAI TTAA 7 cut(s) 807, 981, 1080, 1152, 1167, 1383, 1511
SatI GCNGC 9 cut(s) 66, 75, 90, 402, 412, 925, 1038, 1475, 1560
Sau3AI GATC 6 cut(s) 159, 349, 436, 1448, 1655, 1827
Sau96I GGNCC 4 cut(s) 164, 584, 918, 1717
SchI GAGTC 2 cut(s) 1018, 1856
ScrFI CCNGG 3 cut(s) 647, 921, 945
SduI GDGCHC 2 cut(s) 434, 1540
SfaNI GCATC 3 cut(s) 683, 902, 1864
SfcI CTRYAG 1 cut(s) 60
Sfr274I CTCGAG 1 cut(s) 1006
SinI GGWCC 2 cut(s) 584, 1717
SlaI CTCGAG 1 cut(s) 1006
SmiMI CAYNNNNRTG 3 cut(s) 724, 1374, 1495
SmlI CTYRAG 5 cut(s) 238, 1006, 1157, 1510, 1795
SmoI CTYRAG 5 cut(s) 238, 1006, 1157, 1510, 1795
SpeI ACTAGT 1 cut(s) 746
SsiI CCGC 4 cut(s) 129, 345, 411, 672
SspMI CTAG 7 cut(s) 11, 222, 567, 722, 747, 1610, 1653
SstI GAGCTC 2 cut(s) 434, 1540
StyD4I CCNGG 3 cut(s) 645, 919, 943
StyI CCWWGG 3 cut(s) 423, 1228, 1243
TaaI ACNGT 6 cut(s) 614, 1020, 1134, 1252, 1332, 1556
TaiI ACGT 2 cut(s) 327, 1499
TaqI TCGA 4 cut(s) 846, 1007, 1595, 1736
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 4 cut(s) 80, 393, 1224, 1281
Tru1I TTAA 7 cut(s) 807, 981, 1080, 1152, 1167, 1383, 1511
Tru9I TTAA 7 cut(s) 807, 981, 1080, 1152, 1167, 1383, 1511
TscAI CASTG 6 cut(s) 99, 568, 619, 637, 822, 1207
TseFI GTSAC 3 cut(s) 559, 632, 1820
TseI GCWGC 8 cut(s) 65, 74, 89, 401, 924, 1037, 1474, 1559
Tsp45I GTSAC 3 cut(s) 559, 632, 1820
TspDTI ATGAA 7 cut(s) 5, 950, 1202, 1457, 1568, 1629, 1853
TspGWI ACGGA 3 cut(s) 1125, 1538, 1763
TspRI CASTG 6 cut(s) 99, 568, 619, 637, 822, 1207
Vha464I CTTAAG 1 cut(s) 1510
VpaK11BI GGWCC 2 cut(s) 584, 1717
XbaI TCTAGA 1 cut(s) 1652
XhoI CTCGAG 1 cut(s) 1006
XmiI GTMKAC 5 cut(s) 218, 1254, 1301, 1321, 1595
XspI CTAG 7 cut(s) 11, 222, 567, 722, 747, 1610, 1653
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.