Rw0G004850

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00186
OG0000052
Physical Location & Seq
Reverse (-)
9909 .. 12848
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004850.1

Sequence Viewer

Length: 2256 bp
ATGGTTGCCCAGTTGCTATTTCATATTACTCTGTTACTATGGTCTATGAGTAGTACTGAAGTACTAGTTGTAGCATCAGAAGCACCCATTGTAGCAACAGAAGCACCCATAGCAAAGCCTAATTGTCCATCGCATTGTGGAGGCATTGAAATCCCATACCCTTTTGGGATAGGAGCAGGTTGTTACATTCATGACGACTGGTTTCAAGTATTCTGTGACAACTCCACTGGCTCTCCTACACCTTTCTTGAACGGCACCAATCTGGAGGTACTAGATATTTCTGTTGAAGGCACTCTAAAGGTCAGAAACCCAATCACCTTCTCTAATTGCAGTGATAAGCCAAATCGTCAAGCAGTCAACTTGGAGGGAAGCCCTTTTATGTTCTCCCAGAAGAACATGTTCACTGCAGTAGGTTGCGGAGTTATGGCCACAATCACCTCAAACTCAAATGGCGTTACCATTTCAGCCGGGTGCAGGTCCGAATGTTATGATTATACTGTTAGTAAAAATAGTAGCCGCCATAATACCTGCAATGGTGTGGATTGCTGCCAGACTGTCATCCCTCCTTCCCTCTCTGCCTTCAATACTAGTTTCCAGCAGGCTAATGATAATTACACAAATAGTTCATGTAATTATGCATTTTTGGTTGATCGTGACTGGTTTGAGTCATTTGCCAAAAACTCAACAAAAATTTCTGCCACCGGTGATATGGACCAAGTCCCCGTGGAGCTAAAATGGAACTTATACCATTCAACAACTGATGTTTTTGGGACCTTTATAGAAACAAATGCATCGATGTGGTATGCTGATGACCTAGATGGTCAGTGCGAGACTTACAATGACAGCTCTTCATTATATGGATCCTTAAGGCTTGAATGTTCCTGTGGAGGGGGCTCTAAGGGAAATCCATATCTTCTTCAAGGATGCAGAGATATCAATGAATGTGAGGATCCGGAAAACAGAGCTAGTTGTTCTGCAGGGCTCGTATGTGTCAACTATAATAATGGATGGGGATGCACCTACCCAGAAACCAGCAAGTCGCGGGTTAAAGTAATCTTCATAGGTATCGGCAGTGGTTTTGGACTGATAGTTCTACTAATTGGTGCATGGTGGTTGTACAAACTTTGGAAGAAAAGGAAAAACATTAAACTCAAGAAGAAGTTCTTTAAACAAAATGGGGGACTATTATTAGAGCAGCAACTATCTTCCGGTGAAGTTAATGTTGAGAAAATTAAGCTGTTCAATTCAAAGGAATTAGAAAAAGCCACAGACCGGTTTAATGTAGATAGAATACTCGGCCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGGCAGATGGAAGAATTGTTGCTGTAAAGAAGTCTAAAATAGTTGATGGGGGTGAAGTGGGACAATTCATTAATGAAATTGTCATTCTTTCACAAATTAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACCGAAGTTCCACTTTTGGTTTACGAATTCATACCCAAAGGGACTATCTACCAGTATCTCCATGATCAGAATGAAAAGTTTCCACTCACATGGGAAATGCGCTTAAGGATTTCTGCAGAAGTTGCCGGAGCTCTTTCATACTTACACTCTGCAGCTGCTTTCCCAATTTACCACCGTGATATCAAGTCTACAAACATACTATTAGATGATAAATACAGAGCAAAAGTTGCAGACTTTGGGACTTCAAGATCAGTTTCCATAGACCAAACACACCTAACCACATTTGTACATGGTACATTTGGTTACTTGGATCCGGAGTACTTCCAATCAAGTCAATTTAAAAGTGACGTGTATAGCTTTGGAGTGGTCCTTGTCGAGCTCTTGACTGGAGAAAAACCAGTTTCTCTGACTAGGTCACAAGAAGCCAGAGGCCTTGTTTCATATTTCAATCTCTCACTGGAGTCGAATAATTTGTTTGATATTATTGATGCTCGAGTTAAGGTGGAGGGTGTAACAGGAGATATTTTGACAGTAGCTAATCTTGCAAAAAGATGTCTGGATATGAATGGGAAGAGACGACCTACCATGAAAGAAGTTGCAATGGAGTTGGAGGTGATTCATAAATCAGTGAAATCGTCAGATATACAACAAAATCATGAAGAGGTTGAGTATGTCCGAAATGAAGTAACTTGTCTTTGGGATGTTGCTTCTACATCAACCAGGTCAGCTTTGGATGGTGGCACTGCTTCATCAATTGATTCACTACCACTAATGTCCAACTGA

Protein Analysis

751

Amino Acids

83.02

Weight (kDa)

5.6

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 8.6e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 426 - 690 3.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 428 - 694 1.5e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 167, 465, 536
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 1661
AccII CGCG 1 cut(s) 1042
AccIII TCCGGA 2 cut(s) 952, 1786
AciI CCGC 3 cut(s) 417, 517, 1042
AclWI GGATC 6 cut(s) 855, 868, 944, 957, 1778, 1791
AcoI YGGCCR 2 cut(s) 426, 1297
AcsI RAATTY 2 cut(s) 690, 1499
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 8 cut(s) 55, 63, 270, 1118, 1315, 1761, 1768, 1793
AfiI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
AflII CTTAAG 2 cut(s) 865, 1576
AflIII ACRYGT 2 cut(s) 396, 1821
AgeI ACCGGT 2 cut(s) 701, 1272
AhlI ACTAGT 2 cut(s) 64, 587
AjiI CACGTC 1 cut(s) 1822
AjnI CCWGG 1 cut(s) 2192
AloI GAACNNNNNNTCC 2 cut(s) 1074, 1106
Alw21I GWGCWC 2 cut(s) 1606, 1854
Alw26I GTCTC 3 cut(s) 824, 1466, 2041
AlwI GGATC 6 cut(s) 855, 868, 944, 957, 1778, 1791
Ama87I CYCGRG 1 cut(s) 1965
Aor13HI TCCGGA 2 cut(s) 952, 1786
AoxI GGCC 4 cut(s) 426, 1297, 1306, 1903
ApeKI GCWGC 4 cut(s) 546, 1195, 1625, 1628
ApoI RAATTY 2 cut(s) 690, 1499
AseI ATTAAT 1 cut(s) 1404
AsiGI ACCGGT 2 cut(s) 701, 1272
Asp700I GAANNNNTTC 3 cut(s) 398, 1160, 1551
AspLEI GCGC 1 cut(s) 1575
AspS9I GGNCC 4 cut(s) 477, 712, 771, 1840
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 6 cut(s) 307, 427, 716, 1223, 1397, 2098
AvaI CYCGRG 1 cut(s) 1965
AvaII GGWCC 4 cut(s) 477, 712, 771, 1840
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 3 cut(s) 860, 949, 1783
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 4 cut(s) 896, 984, 1606, 1854
Bbv12I GWGCWC 2 cut(s) 1606, 1854
BbvI GCAGC 4 cut(s) 533, 1207, 1615, 1637
BccI CCATC 6 cut(s) 136, 812, 1002, 1334, 1373, 2201
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 2194
BclI TGATCA 1 cut(s) 1537
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 3 cut(s) 824, 1466, 2041
BcuI ACTAGT 2 cut(s) 64, 587
BfaI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1884
BfmI CTRYAG 4 cut(s) 405, 975, 1587, 1623
BfrI CTTAAG 2 cut(s) 865, 1576
BfuAI ACCTGC 3 cut(s) 167, 465, 536
BglI GCCNNNNNGGC 1 cut(s) 1305
BisI GCNGC 5 cut(s) 517, 547, 1196, 1626, 1629
BlsI GCNGC 5 cut(s) 518, 548, 1197, 1627, 1630
BmcAI AGTACT 3 cut(s) 55, 63, 1793
Bme1390I CCNGG 2 cut(s) 469, 2194
Bme18I GGWCC 4 cut(s) 477, 712, 771, 1840
BmeT110I CYCGRG 1 cut(s) 1965
BmgBI CACGTC 1 cut(s) 1822
BmgT120I GGNCC 4 cut(s) 477, 712, 771, 1840
BmiI GGNNCC 5 cut(s) 256, 772, 862, 951, 1785
BmrFI CCNGG 2 cut(s) 469, 2194
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 5 cut(s) 83, 800, 914, 1004, 1951
BmuI ACTGGG 1 cut(s) 4
BpmI CTGGAG 3 cut(s) 284, 1881, 1952
BpuEI CTTGAG 1 cut(s) 1136
BpuMI CCSGG 1 cut(s) 469
Bsa29I ATCGAT 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 1466
BsaJI CCNNGG 3 cut(s) 723, 1300, 1309
BsaWI WCCGGW 5 cut(s) 701, 952, 1208, 1272, 1786
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 888, 1272
Bse118I RCCGGY 2 cut(s) 701, 1272
Bse1I ACTGG 8 cut(s) 10, 203, 232, 662, 1525, 1864, 1871, 1935
Bse3DI GCAATG 2 cut(s) 538, 2079
BseAI TCCGGA 2 cut(s) 952, 1786
BseBI CCWGG 1 cut(s) 2194
BseCI ATCGAT 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 723, 1300, 1309
BseGI GGATG 6 cut(s) 558, 929, 1013, 1019, 2179, 2212
BseLI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
BseMI GCAATG 2 cut(s) 538, 2079
BseNI ACTGG 8 cut(s) 10, 203, 232, 662, 1525, 1864, 1871, 1935
BseXI GCAGC 4 cut(s) 533, 1207, 1615, 1637
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 4 cut(s) 428, 1299, 1308, 1905
BshNI GGYRCC 1 cut(s) 254
BshTI ACCGGT 2 cut(s) 701, 1272
BshVI ATCGAT 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 1606, 1854
BsiHKCI CYCGRG 1 cut(s) 1965
BsiSI CCGG 7 cut(s) 468, 702, 953, 1209, 1273, 1599, 1787
BslFI GGGAC 6 cut(s) 704, 784, 1194, 1407, 1528, 1726
BslI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
BsmAI GTCTC 3 cut(s) 824, 1466, 2041
BsmBI CGTCTC 1 cut(s) 2041
BsmFI GGGAC 6 cut(s) 704, 784, 1194, 1407, 1528, 1726
BsnI GGCC 4 cut(s) 428, 1299, 1308, 1905
Bso31I GGTCTC 1 cut(s) 1466
BsoBI CYCGRG 1 cut(s) 1965
Bsp1286I GDGCHC 4 cut(s) 896, 984, 1606, 1854
Bsp13I TCCGGA 2 cut(s) 952, 1786
Bsp1407I TGTACA 2 cut(s) 1116, 1759
Bsp143I GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
BspACI CCGC 3 cut(s) 417, 517, 1042
BspANI GGCC 4 cut(s) 428, 1299, 1308, 1905
BspDI ATCGAT 1 cut(s) 794
BspEI TCCGGA 2 cut(s) 952, 1786
BspFNI CGCG 1 cut(s) 1042
BspHI TCATGA 2 cut(s) 190, 2128
BspLI GGNNCC 5 cut(s) 256, 772, 862, 951, 1785
BspMAI CTGCAG 4 cut(s) 409, 979, 1591, 1627
BspMI ACCTGC 3 cut(s) 167, 465, 536
BspPI GGATC 6 cut(s) 855, 868, 944, 957, 1778, 1791
BspQI GCTCTTC 1 cut(s) 853
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 2 cut(s) 865, 1576
BspTNI GGTCTC 1 cut(s) 1466
BsrDI GCAATG 2 cut(s) 538, 2079
BsrFI RCCGGY 2 cut(s) 701, 1272
BsrGI TGTACA 2 cut(s) 1116, 1759
BsrI ACTGG 8 cut(s) 10, 203, 232, 662, 1525, 1864, 1871, 1935
BssAI RCCGGY 2 cut(s) 701, 1272
BssECI CCNNGG 3 cut(s) 723, 1300, 1309
BssMI GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
BssT1I CCWWGG 2 cut(s) 1300, 1309
Bst2UI CCWGG 1 cut(s) 2194
Bst4CI ACNGT 5 cut(s) 499, 556, 1318, 1649, 2005
Bst6I CTCTTC 3 cut(s) 853, 2039, 2127
BstAFI CTTAAG 2 cut(s) 865, 1576
BstAPI GCANNNNNTGC 2 cut(s) 1595, 1700
BstAUI TGTACA 2 cut(s) 1116, 1759
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 1 cut(s) 897
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 6 cut(s) 558, 929, 1013, 1019, 2179, 2212
BstFNI CGCG 1 cut(s) 1042
BstHHI GCGC 1 cut(s) 1575
BstKTI GATC 6 cut(s) 652, 863, 952, 1540, 1724, 1786
BstMAI GTCTC 3 cut(s) 824, 1466, 2041
BstMBI GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
BstMWI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1595, 1700, 2015
BstNI CCWGG 1 cut(s) 2194
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 467, 2192
BstSFI CTRYAG 4 cut(s) 405, 975, 1587, 1623
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 4 cut(s) 533, 1207, 1615, 1637
BstX2I RGATCY 3 cut(s) 860, 949, 1783
BstXI CCANNNNNNTGG 1 cut(s) 1563
BstYI RGATCY 3 cut(s) 860, 949, 1783
Bsu15I ATCGAT 1 cut(s) 794
BsuRI GGCC 4 cut(s) 428, 1299, 1308, 1905
BsuTUI ATCGAT 1 cut(s) 794
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1822
BtsCI GGATG 6 cut(s) 558, 929, 1013, 1019, 2179, 2212
BtsI GCAGTG 4 cut(s) 337, 402, 1078, 2214
BtsIMutI CAGTG 8 cut(s) 225, 337, 402, 830, 1078, 1928, 2106, 2214
BveI ACCTGC 3 cut(s) 167, 465, 536
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 2 cut(s) 190, 2128
CfoI GCGC 1 cut(s) 1575
Cfr10I RCCGGY 2 cut(s) 701, 1272
Cfr13I GGNCC 4 cut(s) 477, 712, 771, 1840
ClaI ATCGAT 1 cut(s) 794
CsiI ACCWGGT 1 cut(s) 2192
Csp6I GTAC 8 cut(s) 54, 62, 269, 1117, 1314, 1760, 1767, 1792
CspAI ACCGGT 2 cut(s) 701, 1272
CviAII CATG 9 cut(s) 191, 397, 627, 1107, 1535, 1563, 1763, 2059, 2129
CviQI GTAC 8 cut(s) 54, 62, 269, 1117, 1314, 1760, 1767, 1792
DdeI CTNAG 1 cut(s) 897
DpnI GATC 6 cut(s) 651, 862, 951, 1539, 1723, 1785
DpnII GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
DraI TTTAAA 2 cut(s) 1168, 1813
EaeI YGGCCR 2 cut(s) 426, 1297
Eam1104I CTCTTC 3 cut(s) 853, 2039, 2127
EarI CTCTTC 3 cut(s) 853, 2039, 2127
Ecl136II GAGCTC 2 cut(s) 1604, 1852
Eco130I CCWWGG 2 cut(s) 1300, 1309
Eco147I AGGCCT 1 cut(s) 1905
Eco24I GRGCYC 4 cut(s) 896, 984, 1606, 1854
Eco31I GGTCTC 1 cut(s) 1466
Eco32I GATATC 2 cut(s) 934, 1654
Eco47I GGWCC 4 cut(s) 477, 712, 771, 1840
Eco53kI GAGCTC 2 cut(s) 1604, 1852
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1965
EcoICRI GAGCTC 2 cut(s) 1604, 1852
EcoO109I RGGNCCY 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 1499
EcoRII CCWGG 1 cut(s) 2192
EcoRV GATATC 2 cut(s) 934, 1654
EcoT14I CCWWGG 2 cut(s) 1300, 1309
EcoT22I ATGCAT 2 cut(s) 640, 793
EcoT38I GRGCYC 4 cut(s) 896, 984, 1606, 1854
ErhI CCWWGG 2 cut(s) 1300, 1309
Esp3I CGTCTC 1 cut(s) 2041
FaeI CATG 9 cut(s) 194, 400, 630, 1110, 1538, 1566, 1766, 2062, 2132
FalI AAGNNNNNCTT 4 cut(s) 1148, 1180, 1470, 1502
FaqI GGGAC 6 cut(s) 704, 784, 1194, 1407, 1528, 1726
FatI CATG 9 cut(s) 190, 396, 626, 1106, 1534, 1562, 1762, 2058, 2128
FauI CCCGC 1 cut(s) 1035
FbaI TGATCA 1 cut(s) 1537
FblI GTMKAC 1 cut(s) 1661
Fnu4HI GCNGC 5 cut(s) 517, 547, 1196, 1626, 1629
FokI GGATG 6 cut(s) 545, 936, 1020, 1026, 2186, 2219
FriOI GRGCYC 4 cut(s) 896, 984, 1606, 1854
Fsp4HI GCNGC 5 cut(s) 517, 547, 1196, 1626, 1629
FspBI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1884
GlaI GCGC 1 cut(s) 1574
GluI GCNGC 5 cut(s) 517, 547, 1196, 1626, 1629
GsuI CTGGAG 3 cut(s) 284, 1881, 1952
HaeIII GGCC 4 cut(s) 428, 1299, 1308, 1905
HapII CCGG 7 cut(s) 468, 702, 953, 1209, 1273, 1599, 1787
HhaI GCGC 1 cut(s) 1575
Hin1II CATG 9 cut(s) 194, 400, 630, 1110, 1538, 1566, 1766, 2062, 2132
Hin6I GCGC 1 cut(s) 1573
HinP1I GCGC 1 cut(s) 1573
HincII GTYRAC 2 cut(s) 358, 994
HindII GTYRAC 2 cut(s) 358, 994
HinfI GANTC 4 cut(s) 665, 1934, 2089, 2231
HpaII CCGG 7 cut(s) 468, 702, 953, 1209, 1273, 1599, 1787
HphI GGTGA 6 cut(s) 307, 427, 716, 1223, 1397, 2098
Hpy166II GTNNAC 6 cut(s) 358, 402, 994, 1321, 1495, 1662
Hpy188I TCNGA 7 cut(s) 79, 305, 481, 1542, 1881, 2113, 2150
Hpy8I GTNNAC 6 cut(s) 358, 402, 994, 1321, 1495, 1662
HpyAV CCTTC 4 cut(s) 281, 328, 576, 589
HpyCH4III ACNGT 5 cut(s) 499, 556, 1318, 1649, 2005
HpyCH4IV ACGT 1 cut(s) 1821
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1595, 1700, 2015
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 1 cut(s) 1821
Hsp92II CATG 9 cut(s) 194, 400, 630, 1110, 1538, 1566, 1766, 2062, 2132
HspAI GCGC 1 cut(s) 1573
Kpn2I TCCGGA 2 cut(s) 952, 1786
Ksp22I TGATCA 1 cut(s) 1537
Kzo9I GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 3 cut(s) 173, 727, 1601
Lsp1109I GCAGC 4 cut(s) 533, 1207, 1615, 1637
LweI GCATC 5 cut(s) 83, 800, 914, 1004, 1951
MabI ACCWGGT 1 cut(s) 2192
MaeI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1884
MaeII ACGT 1 cut(s) 1821
MalI GATC 6 cut(s) 651, 862, 951, 1539, 1723, 1785
MboI GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
MfeI CAATTG 1 cut(s) 2226
MflI RGATCY 3 cut(s) 860, 949, 1783
MhlI GDGCHC 4 cut(s) 896, 984, 1606, 1854
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 674, 1943
MmeI TCCRAC 1 cut(s) 2061
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 2 cut(s) 640, 793
MroI TCCGGA 2 cut(s) 952, 1786
MroXI GAANNNNTTC 3 cut(s) 398, 1160, 1551
MscI TGGCCA 1 cut(s) 428
MslI CAYNNNNRTG 3 cut(s) 796, 1440, 1561
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 1628
MspCI CTTAAG 2 cut(s) 865, 1576
MspI CCGG 7 cut(s) 468, 702, 953, 1209, 1273, 1599, 1787
MspR9I CCNGG 2 cut(s) 469, 2194
MunI CAATTG 1 cut(s) 2226
MvaI CCWGG 1 cut(s) 2194
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1595, 1700, 2015
NciI CCSGG 1 cut(s) 469
NdeII GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
NlaIII CATG 9 cut(s) 194, 400, 630, 1110, 1538, 1566, 1766, 2062, 2132
NlaIV GGNNCC 5 cut(s) 256, 772, 862, 951, 1785
NmeAIII GCCGAG 1 cut(s) 1275
NmuCI GTSAC 4 cut(s) 215, 653, 1817, 1887
NsiI ATGCAT 2 cut(s) 640, 793
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 1965
PagI TCATGA 2 cut(s) 190, 2128
PceI AGGCCT 1 cut(s) 1905
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 853
PdmI GAANNNNTTC 3 cut(s) 398, 1160, 1551
PfeI GAWTC 2 cut(s) 2089, 2231
PflFI GACNNNGTC 2 cut(s) 716, 1885
PinAI ACCGGT 2 cut(s) 701, 1272
PkrI GCNGC 5 cut(s) 518, 548, 1197, 1627, 1630
PleI GAGTC 2 cut(s) 673, 1942
PpsI GAGTC 2 cut(s) 673, 1942
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 396
PshBI ATTAAT 1 cut(s) 1404
Psp124BI GAGCTC 2 cut(s) 1606, 1854
Psp5II RGGWCCY 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 2192
PspGI CCWGG 1 cut(s) 2192
PspN4I GGNNCC 5 cut(s) 256, 772, 862, 951, 1785
PspPI GGNCC 4 cut(s) 477, 712, 771, 1840
PspPPI RGGWCCY 1 cut(s) 771
PspXI VCTCGAGB 1 cut(s) 1965
PstI CTGCAG 4 cut(s) 409, 979, 1591, 1627
PsuI RGATCY 3 cut(s) 860, 949, 1783
PsyI GACNNNGTC 2 cut(s) 716, 1885
PvuII CAGCTG 1 cut(s) 1628
RsaI GTAC 8 cut(s) 55, 63, 270, 1118, 1315, 1761, 1768, 1793
RsaNI GTAC 8 cut(s) 54, 62, 269, 1117, 1314, 1760, 1767, 1792
RseI CAYNNNNRTG 3 cut(s) 796, 1440, 1561
SacI GAGCTC 2 cut(s) 1606, 1854
SapI GCTCTTC 1 cut(s) 853
SatI GCNGC 5 cut(s) 517, 547, 1196, 1626, 1629
Sau3AI GATC 6 cut(s) 649, 860, 949, 1537, 1721, 1783
Sau96I GGNCC 4 cut(s) 477, 712, 771, 1840
ScaI AGTACT 3 cut(s) 55, 63, 1793
SchI GAGTC 2 cut(s) 674, 1943
ScrFI CCNGG 2 cut(s) 469, 2194
SduI GDGCHC 4 cut(s) 896, 984, 1606, 1854
SexAI ACCWGGT 1 cut(s) 2192
SfaNI GCATC 5 cut(s) 83, 800, 914, 1004, 1951
SfcI CTRYAG 4 cut(s) 405, 975, 1587, 1623
SfiI GGCCNNNNNGGCC 1 cut(s) 1305
Sfr274I CTCGAG 1 cut(s) 1965
SgrAI CRCCGGYG 1 cut(s) 701
SinI GGWCC 4 cut(s) 477, 712, 771, 1840
SlaI CTCGAG 1 cut(s) 1965
SmiMI CAYNNNNRTG 3 cut(s) 796, 1440, 1561
SmlI CTYRAG 4 cut(s) 865, 1151, 1576, 1965
SmoI CTYRAG 4 cut(s) 865, 1151, 1576, 1965
SpeI ACTAGT 2 cut(s) 64, 587
SseBI AGGCCT 1 cut(s) 1905
SsiI CCGC 3 cut(s) 417, 517, 1042
SspMI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1884
SstI GAGCTC 2 cut(s) 1606, 1854
StuI AGGCCT 1 cut(s) 1905
StyD4I CCNGG 2 cut(s) 467, 2192
StyI CCWWGG 2 cut(s) 1300, 1309
TaaI ACNGT 5 cut(s) 499, 556, 1318, 1649, 2005
TaiI ACGT 1 cut(s) 1824
TaqI TCGA 4 cut(s) 794, 1848, 1937, 1966
TaqII GACCGA 1 cut(s) 1490
TatI WGTACW 5 cut(s) 53, 61, 1116, 1759, 1791
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 2 cut(s) 2089, 2231
TscAI CASTG 8 cut(s) 232, 337, 409, 830, 1078, 1935, 2106, 2221
TseFI GTSAC 4 cut(s) 215, 653, 1817, 1887
TseI GCWGC 4 cut(s) 546, 1195, 1625, 1628
Tsp45I GTSAC 4 cut(s) 215, 653, 1817, 1887
TspRI CASTG 8 cut(s) 232, 337, 409, 830, 1078, 1935, 2106, 2221
Tth111I GACNNNGTC 2 cut(s) 716, 1885
Vha464I CTTAAG 2 cut(s) 865, 1576
VpaK11BI GGWCC 4 cut(s) 477, 712, 771, 1840
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 2 cut(s) 690, 1499
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 706, 2200
XhoI CTCGAG 1 cut(s) 1965
XmiI GTMKAC 1 cut(s) 1661
XmnI GAANNNNTTC 3 cut(s) 398, 1160, 1551
XspI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1884
ZrmI AGTACT 3 cut(s) 55, 63, 1793
Zsp2I ATGCAT 2 cut(s) 640, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.