wall-associated receptor kinase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 3 cut(s) | 167, 465, 536 |
| AccB1I | GGYRCC | 1 cut(s) | 254 |
| AccI | GTMKAC | 2 cut(s) | 210, 1658 |
| AccII | CGCG | 1 cut(s) | 1039 |
| AccIII | TCCGGA | 1 cut(s) | 949 |
| AciI | CCGC | 1 cut(s) | 1039 |
| AclWI | GGATC | 4 cut(s) | 941, 954, 1775, 1788 |
| AcoI | YGGCCR | 3 cut(s) | 426, 1294, 1332 |
| AcsI | RAATTY | 2 cut(s) | 679, 1496 |
| AcuI | CTGAAG | 1 cut(s) | 78 |
| AfaI | GTAC | 7 cut(s) | 55, 270, 1115, 1312, 1758, 1765, 1790 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 474, 885, 1269 |
| AflII | CTTAAG | 2 cut(s) | 862, 1573 |
| AflIII | ACRYGT | 2 cut(s) | 396, 1824 |
| AgeI | ACCGGT | 1 cut(s) | 1269 |
| AhlI | ACTAGT | 2 cut(s) | 64, 587 |
| AjiI | CACGTC | 1 cut(s) | 1825 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1071, 1103 |
| Alw21I | GWGCWC | 2 cut(s) | 1603, 1857 |
| Alw26I | GTCTC | 3 cut(s) | 821, 1463, 2044 |
| AlwI | GGATC | 4 cut(s) | 941, 954, 1775, 1788 |
| Ama87I | CYCGRG | 1 cut(s) | 1968 |
| Aor13HI | TCCGGA | 1 cut(s) | 949 |
| AoxI | GGCC | 5 cut(s) | 426, 1294, 1303, 1332, 1906 |
| ApeKI | GCWGC | 4 cut(s) | 546, 1192, 1622, 1625 |
| ApoI | RAATTY | 2 cut(s) | 679, 1496 |
| AseI | ATTAAT | 1 cut(s) | 1401 |
| AsiGI | ACCGGT | 1 cut(s) | 1269 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 398, 1157, 1548 |
| AspLEI | GCGC | 2 cut(s) | 1103, 1572 |
| AspS9I | GGNCC | 4 cut(s) | 477, 712, 771, 1843 |
| AsuC2I | CCSGG | 2 cut(s) | 469, 2197 |
| AsuHPI | GGTGA | 5 cut(s) | 307, 427, 818, 1220, 2101 |
| AvaI | CYCGRG | 1 cut(s) | 1968 |
| AvaII | GGWCC | 4 cut(s) | 477, 712, 771, 1843 |
| BalI | TGGCCA | 1 cut(s) | 428 |
| BamHI | GGATCC | 2 cut(s) | 946, 1780 |
| BanI | GGYRCC | 1 cut(s) | 254 |
| BanII | GRGCYC | 4 cut(s) | 893, 981, 1603, 1857 |
| Bbv12I | GWGCWC | 2 cut(s) | 1603, 1857 |
| BbvI | GCAGC | 4 cut(s) | 533, 1204, 1612, 1634 |
| BccI | CCATC | 6 cut(s) | 136, 809, 999, 1331, 1370, 2204 |
| BceAI | ACGGC | 1 cut(s) | 268 |
| BcnI | CCSGG | 2 cut(s) | 469, 2197 |
| BcoDI | GTCTC | 3 cut(s) | 821, 1463, 2044 |
| BcuI | ACTAGT | 2 cut(s) | 64, 587 |
| BfaI | CTAG | 5 cut(s) | 65, 588, 812, 963, 1887 |
| BfmI | CTRYAG | 4 cut(s) | 405, 972, 1584, 1620 |
| BfrI | CTTAAG | 2 cut(s) | 862, 1573 |
| BfuAI | ACCTGC | 3 cut(s) | 167, 465, 536 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1302 |
| BisI | GCNGC | 4 cut(s) | 547, 1193, 1623, 1626 |
| BlsI | GCNGC | 4 cut(s) | 548, 1194, 1624, 1627 |
| BmcAI | AGTACT | 2 cut(s) | 55, 1790 |
| Bme1390I | CCNGG | 2 cut(s) | 469, 2197 |
| Bme18I | GGWCC | 4 cut(s) | 477, 712, 771, 1843 |
| BmeT110I | CYCGRG | 1 cut(s) | 1968 |
| BmgBI | CACGTC | 1 cut(s) | 1825 |
| BmgT120I | GGNCC | 4 cut(s) | 477, 712, 771, 1843 |
| BmiI | GGNNCC | 4 cut(s) | 256, 772, 948, 1782 |
| BmrFI | CCNGG | 2 cut(s) | 469, 2197 |
| BmrI | ACTGGG | 1 cut(s) | 4 |
| BmsI | GCATC | 5 cut(s) | 83, 800, 911, 1001, 1954 |
| BmuI | ACTGGG | 1 cut(s) | 4 |
| BpmI | CTGGAG | 3 cut(s) | 284, 1884, 1955 |
| BpuEI | CTTGAG | 2 cut(s) | 900, 1133 |
| BpuMI | CCSGG | 2 cut(s) | 469, 2197 |
| BsaI | GGTCTC | 1 cut(s) | 1463 |
| BsaJI | CCNNGG | 4 cut(s) | 723, 1297, 1306, 2171 |
| BsaWI | WCCGGW | 3 cut(s) | 949, 1205, 1269 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 217, 247, 1371, 1401 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 474, 885, 1269 |
| Bse118I | RCCGGY | 1 cut(s) | 1269 |
| Bse3DI | GCAATG | 2 cut(s) | 538, 2082 |
| BseAI | TCCGGA | 1 cut(s) | 949 |
| BseDI | CCNNGG | 4 cut(s) | 723, 1297, 1306, 2171 |
| BseGI | GGATG | 6 cut(s) | 558, 926, 1010, 1016, 2182, 2215 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 474, 885, 1269 |
| BseMI | GCAATG | 2 cut(s) | 538, 2082 |
| BseXI | GCAGC | 4 cut(s) | 533, 1204, 1612, 1634 |
| BsgI | GTGCAG | 1 cut(s) | 493 |
| Bsh1236I | CGCG | 1 cut(s) | 1039 |
| BshFI | GGCC | 5 cut(s) | 428, 1296, 1305, 1334, 1908 |
| BshNI | GGYRCC | 1 cut(s) | 254 |
| BshTI | ACCGGT | 1 cut(s) | 1269 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1603, 1857 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1968 |
| BsiSI | CCGG | 6 cut(s) | 468, 950, 1206, 1270, 1596, 2196 |
| BslFI | GGGAC | 6 cut(s) | 704, 784, 1191, 1404, 1525, 1723 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 474, 885, 1269 |
| BsmAI | GTCTC | 3 cut(s) | 821, 1463, 2044 |
| BsmBI | CGTCTC | 1 cut(s) | 2044 |
| BsmFI | GGGAC | 6 cut(s) | 704, 784, 1191, 1404, 1525, 1723 |
| BsnI | GGCC | 5 cut(s) | 428, 1296, 1305, 1334, 1908 |
| Bso31I | GGTCTC | 1 cut(s) | 1463 |
| BsoBI | CYCGRG | 1 cut(s) | 1968 |
| Bsp1286I | GDGCHC | 4 cut(s) | 893, 981, 1603, 1857 |
| Bsp13I | TCCGGA | 1 cut(s) | 949 |
| Bsp1407I | TGTACA | 2 cut(s) | 1113, 1756 |
| Bsp143I | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| BspACI | CCGC | 1 cut(s) | 1039 |
| BspANI | GGCC | 5 cut(s) | 428, 1296, 1305, 1334, 1908 |
| BspEI | TCCGGA | 1 cut(s) | 949 |
| BspFNI | CGCG | 1 cut(s) | 1039 |
| BspHI | TCATGA | 2 cut(s) | 190, 2131 |
| BspLI | GGNNCC | 4 cut(s) | 256, 772, 948, 1782 |
| BspMAI | CTGCAG | 4 cut(s) | 409, 976, 1588, 1624 |
| BspMI | ACCTGC | 3 cut(s) | 167, 465, 536 |
| BspPI | GGATC | 4 cut(s) | 941, 954, 1775, 1788 |
| BspT107I | GGYRCC | 1 cut(s) | 254 |
| BspTI | CTTAAG | 2 cut(s) | 862, 1573 |
| BspTNI | GGTCTC | 1 cut(s) | 1463 |
| BsrDI | GCAATG | 2 cut(s) | 538, 2082 |
| BsrFI | RCCGGY | 1 cut(s) | 1269 |
| BsrGI | TGTACA | 2 cut(s) | 1113, 1756 |
| BssAI | RCCGGY | 1 cut(s) | 1269 |
| BssECI | CCNNGG | 4 cut(s) | 723, 1297, 1306, 2171 |
| BssMI | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| BssNAI | GTATAC | 1 cut(s) | 211 |
| BssT1I | CCWWGG | 3 cut(s) | 1297, 1306, 2171 |
| Bst1107I | GTATAC | 1 cut(s) | 211 |
| Bst4CI | ACNGT | 6 cut(s) | 39, 215, 556, 1315, 1646, 2008 |
| Bst6I | CTCTTC | 3 cut(s) | 1539, 2042, 2130 |
| BstAFI | CTTAAG | 2 cut(s) | 862, 1573 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1592, 1697 |
| BstAUI | TGTACA | 2 cut(s) | 1113, 1756 |
| BstC8I | GCNNGC | 2 cut(s) | 517, 600 |
| BstDSI | CCRYGG | 1 cut(s) | 723 |
| BstEII | GGTNACC | 1 cut(s) | 806 |
| BstF5I | GGATG | 6 cut(s) | 558, 926, 1010, 1016, 2182, 2215 |
| BstFNI | CGCG | 1 cut(s) | 1039 |
| BstHHI | GCGC | 2 cut(s) | 1103, 1572 |
| BstKTI | GATC | 4 cut(s) | 652, 949, 1721, 1783 |
| BstMAI | GTCTC | 3 cut(s) | 821, 1463, 2044 |
| BstMBI | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 80, 101, 110, 1302, 1592, 1697, 2018 |
| BstNSI | RCATGY | 1 cut(s) | 400 |
| BstPI | GGTNACC | 1 cut(s) | 806 |
| BstSCI | CCNGG | 2 cut(s) | 467, 2195 |
| BstSFI | CTRYAG | 4 cut(s) | 405, 972, 1584, 1620 |
| BstUI | CGCG | 1 cut(s) | 1039 |
| BstV1I | GCAGC | 4 cut(s) | 533, 1204, 1612, 1634 |
| BstX2I | RGATCY | 2 cut(s) | 946, 1780 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 709, 1560 |
| BstYI | RGATCY | 2 cut(s) | 946, 1780 |
| BstZ17I | GTATAC | 1 cut(s) | 211 |
| BsuRI | GGCC | 5 cut(s) | 428, 1296, 1305, 1334, 1908 |
| BtgI | CCRYGG | 1 cut(s) | 723 |
| BtgZI | GCGATG | 1 cut(s) | 114 |
| BtrI | CACGTC | 1 cut(s) | 1825 |
| BtsCI | GGATG | 6 cut(s) | 558, 926, 1010, 1016, 2182, 2215 |
| BtsI | GCAGTG | 4 cut(s) | 337, 402, 1075, 2217 |
| BtsIMutI | CAGTG | 8 cut(s) | 225, 337, 402, 709, 1075, 1931, 2109, 2217 |
| BveI | ACCTGC | 3 cut(s) | 167, 465, 536 |
| Cac8I | GCNNGC | 2 cut(s) | 517, 600 |
| CciI | TCATGA | 2 cut(s) | 190, 2131 |
| CfoI | GCGC | 2 cut(s) | 1103, 1572 |
| Cfr10I | RCCGGY | 1 cut(s) | 1269 |
| Cfr13I | GGNCC | 4 cut(s) | 477, 712, 771, 1843 |
| Csp6I | GTAC | 7 cut(s) | 54, 269, 1114, 1311, 1757, 1764, 1789 |
| CspAI | ACCGGT | 1 cut(s) | 1269 |
| CviAII | CATG | 8 cut(s) | 191, 397, 627, 1532, 1560, 1760, 2062, 2132 |
| CviQI | GTAC | 7 cut(s) | 54, 269, 1114, 1311, 1757, 1764, 1789 |
| DpnI | GATC | 4 cut(s) | 651, 948, 1720, 1782 |
| DpnII | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| DraI | TTTAAA | 1 cut(s) | 1165 |
| EaeI | YGGCCR | 3 cut(s) | 426, 1294, 1332 |
| Eam1104I | CTCTTC | 3 cut(s) | 1539, 2042, 2130 |
| EarI | CTCTTC | 3 cut(s) | 1539, 2042, 2130 |
| Ecl136II | GAGCTC | 2 cut(s) | 1601, 1855 |
| Eco130I | CCWWGG | 3 cut(s) | 1297, 1306, 2171 |
| Eco147I | AGGCCT | 1 cut(s) | 1908 |
| Eco24I | GRGCYC | 4 cut(s) | 893, 981, 1603, 1857 |
| Eco31I | GGTCTC | 1 cut(s) | 1463 |
| Eco32I | GATATC | 2 cut(s) | 931, 1651 |
| Eco47I | GGWCC | 4 cut(s) | 477, 712, 771, 1843 |
| Eco53kI | GAGCTC | 2 cut(s) | 1601, 1855 |
| Eco57I | CTGAAG | 1 cut(s) | 78 |
| Eco88I | CYCGRG | 1 cut(s) | 1968 |
| Eco91I | GGTNACC | 1 cut(s) | 806 |
| EcoICRI | GAGCTC | 2 cut(s) | 1601, 1855 |
| EcoO109I | RGGNCCY | 1 cut(s) | 771 |
| EcoO65I | GGTNACC | 1 cut(s) | 806 |
| EcoRI | GAATTC | 1 cut(s) | 1496 |
| EcoRV | GATATC | 2 cut(s) | 931, 1651 |
| EcoT14I | CCWWGG | 3 cut(s) | 1297, 1306, 2171 |
| EcoT22I | ATGCAT | 3 cut(s) | 640, 793, 926 |
| EcoT38I | GRGCYC | 4 cut(s) | 893, 981, 1603, 1857 |
| ErhI | CCWWGG | 3 cut(s) | 1297, 1306, 2171 |
| Esp3I | CGTCTC | 1 cut(s) | 2044 |
| FaeI | CATG | 8 cut(s) | 194, 400, 630, 1535, 1563, 1763, 2065, 2135 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1145, 1177, 1467, 1499 |
| FaqI | GGGAC | 6 cut(s) | 704, 784, 1191, 1404, 1525, 1723 |
| FatI | CATG | 8 cut(s) | 190, 396, 626, 1531, 1559, 1759, 2061, 2131 |
| FauI | CCCGC | 1 cut(s) | 1032 |
| FblI | GTMKAC | 2 cut(s) | 210, 1658 |
| Fnu4HI | GCNGC | 4 cut(s) | 547, 1193, 1623, 1626 |
| FokI | GGATG | 6 cut(s) | 545, 933, 1017, 1023, 2189, 2222 |
| FriOI | GRGCYC | 4 cut(s) | 893, 981, 1603, 1857 |
| Fsp4HI | GCNGC | 4 cut(s) | 547, 1193, 1623, 1626 |
| FspBI | CTAG | 5 cut(s) | 65, 588, 812, 963, 1887 |
| GlaI | GCGC | 2 cut(s) | 1102, 1571 |
| GluI | GCNGC | 4 cut(s) | 547, 1193, 1623, 1626 |
| GsuI | CTGGAG | 3 cut(s) | 284, 1884, 1955 |
| HaeIII | GGCC | 5 cut(s) | 428, 1296, 1305, 1334, 1908 |
| HapII | CCGG | 6 cut(s) | 468, 950, 1206, 1270, 1596, 2196 |
| HhaI | GCGC | 2 cut(s) | 1103, 1572 |
| Hin1II | CATG | 8 cut(s) | 194, 400, 630, 1535, 1563, 1763, 2065, 2135 |
| Hin6I | GCGC | 2 cut(s) | 1101, 1570 |
| HinP1I | GCGC | 2 cut(s) | 1101, 1570 |
| HincII | GTYRAC | 2 cut(s) | 358, 991 |
| HindII | GTYRAC | 2 cut(s) | 358, 991 |
| HinfI | GANTC | 6 cut(s) | 491, 665, 857, 1937, 2092, 2234 |
| HpaII | CCGG | 6 cut(s) | 468, 950, 1206, 1270, 1596, 2196 |
| HphI | GGTGA | 5 cut(s) | 307, 427, 818, 1220, 2101 |
| Hpy166II | GTNNAC | 7 cut(s) | 211, 358, 402, 991, 1318, 1492, 1659 |
| Hpy188I | TCNGA | 6 cut(s) | 79, 305, 481, 1884, 2116, 2153 |
| Hpy8I | GTNNAC | 7 cut(s) | 211, 358, 402, 991, 1318, 1492, 1659 |
| HpyAV | CCTTC | 4 cut(s) | 281, 328, 576, 589 |
| HpyCH4III | ACNGT | 6 cut(s) | 39, 215, 556, 1315, 1646, 2008 |
| HpyCH4IV | ACGT | 1 cut(s) | 1824 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 80, 101, 110, 1302, 1592, 1697, 2018 |
| HpySE526I | ACGT | 1 cut(s) | 1824 |
| Hsp92II | CATG | 8 cut(s) | 194, 400, 630, 1535, 1563, 1763, 2065, 2135 |
| HspAI | GCGC | 2 cut(s) | 1101, 1570 |
| Kpn2I | TCCGGA | 1 cut(s) | 949 |
| Kzo9I | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| LmnI | GCTCC | 3 cut(s) | 173, 727, 1598 |
| Lsp1109I | GCAGC | 4 cut(s) | 533, 1204, 1612, 1634 |
| LweI | GCATC | 5 cut(s) | 83, 800, 911, 1001, 1954 |
| MaeI | CTAG | 5 cut(s) | 65, 588, 812, 963, 1887 |
| MaeII | ACGT | 1 cut(s) | 1824 |
| MalI | GATC | 4 cut(s) | 651, 948, 1720, 1782 |
| MboI | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| MfeI | CAATTG | 1 cut(s) | 2229 |
| MflI | RGATCY | 2 cut(s) | 946, 1780 |
| MhlI | GDGCHC | 4 cut(s) | 893, 981, 1603, 1857 |
| MlsI | TGGCCA | 1 cut(s) | 428 |
| MluNI | TGGCCA | 1 cut(s) | 428 |
| MlyI | GAGTC | 2 cut(s) | 674, 1946 |
| MmeI | TCCRAC | 1 cut(s) | 2064 |
| Mox20I | TGGCCA | 1 cut(s) | 428 |
| Mph1103I | ATGCAT | 3 cut(s) | 640, 793, 926 |
| MroI | TCCGGA | 1 cut(s) | 949 |
| MroXI | GAANNNNTTC | 3 cut(s) | 398, 1157, 1548 |
| MscI | TGGCCA | 1 cut(s) | 428 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1437, 1558 |
| Msp20I | TGGCCA | 1 cut(s) | 428 |
| MspA1I | CMGCKG | 1 cut(s) | 1625 |
| MspCI | CTTAAG | 2 cut(s) | 862, 1573 |
| MspI | CCGG | 6 cut(s) | 468, 950, 1206, 1270, 1596, 2196 |
| MspR9I | CCNGG | 2 cut(s) | 469, 2197 |
| MunI | CAATTG | 1 cut(s) | 2229 |
| MvnI | CGCG | 1 cut(s) | 1039 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 80, 101, 110, 1302, 1592, 1697, 2018 |
| NciI | CCSGG | 2 cut(s) | 469, 2197 |
| NdeII | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| NlaIII | CATG | 8 cut(s) | 194, 400, 630, 1535, 1563, 1763, 2065, 2135 |
| NlaIV | GGNNCC | 4 cut(s) | 256, 772, 948, 1782 |
| NmeAIII | GCCGAG | 1 cut(s) | 1272 |
| NmuCI | GTSAC | 5 cut(s) | 215, 653, 806, 1820, 1890 |
| NsiI | ATGCAT | 3 cut(s) | 640, 793, 926 |
| NspI | RCATGY | 1 cut(s) | 400 |
| PaeR7I | CTCGAG | 1 cut(s) | 1968 |
| PagI | TCATGA | 2 cut(s) | 190, 2131 |
| PceI | AGGCCT | 1 cut(s) | 1908 |
| PciI | ACATGT | 1 cut(s) | 396 |
| PdmI | GAANNNNTTC | 3 cut(s) | 398, 1157, 1548 |
| PfeI | GAWTC | 4 cut(s) | 491, 857, 2092, 2234 |
| PflFI | GACNNNGTC | 2 cut(s) | 716, 1888 |
| PinAI | ACCGGT | 1 cut(s) | 1269 |
| PkrI | GCNGC | 4 cut(s) | 548, 1194, 1624, 1627 |
| PleI | GAGTC | 2 cut(s) | 673, 1945 |
| PpsI | GAGTC | 2 cut(s) | 673, 1945 |
| PpuMI | RGGWCCY | 1 cut(s) | 771 |
| PscI | ACATGT | 1 cut(s) | 396 |
| PshBI | ATTAAT | 1 cut(s) | 1401 |
| Psp124BI | GAGCTC | 2 cut(s) | 1603, 1857 |
| Psp5II | RGGWCCY | 1 cut(s) | 771 |
| PspEI | GGTNACC | 1 cut(s) | 806 |
| PspN4I | GGNNCC | 4 cut(s) | 256, 772, 948, 1782 |
| PspPI | GGNCC | 4 cut(s) | 477, 712, 771, 1843 |
| PspPPI | RGGWCCY | 1 cut(s) | 771 |
| PspXI | VCTCGAGB | 1 cut(s) | 1968 |
| PstI | CTGCAG | 4 cut(s) | 409, 976, 1588, 1624 |
| PsuI | RGATCY | 2 cut(s) | 946, 1780 |
| PsyI | GACNNNGTC | 2 cut(s) | 716, 1888 |
| PvuII | CAGCTG | 1 cut(s) | 1625 |
| RsaI | GTAC | 7 cut(s) | 55, 270, 1115, 1312, 1758, 1765, 1790 |
| RsaNI | GTAC | 7 cut(s) | 54, 269, 1114, 1311, 1757, 1764, 1789 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1437, 1558 |
| SacI | GAGCTC | 2 cut(s) | 1603, 1857 |
| SatI | GCNGC | 4 cut(s) | 547, 1193, 1623, 1626 |
| Sau3AI | GATC | 4 cut(s) | 649, 946, 1718, 1780 |
| Sau96I | GGNCC | 4 cut(s) | 477, 712, 771, 1843 |
| ScaI | AGTACT | 2 cut(s) | 55, 1790 |
| SchI | GAGTC | 2 cut(s) | 674, 1946 |
| ScrFI | CCNGG | 2 cut(s) | 469, 2197 |
| SduI | GDGCHC | 4 cut(s) | 893, 981, 1603, 1857 |
| SfaNI | GCATC | 5 cut(s) | 83, 800, 911, 1001, 1954 |
| SfcI | CTRYAG | 4 cut(s) | 405, 972, 1584, 1620 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1302 |
| Sfr274I | CTCGAG | 1 cut(s) | 1968 |
| SinI | GGWCC | 4 cut(s) | 477, 712, 771, 1843 |
| SlaI | CTCGAG | 1 cut(s) | 1968 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1437, 1558 |
| SmlI | CTYRAG | 5 cut(s) | 862, 915, 1148, 1573, 1968 |
| SmoI | CTYRAG | 5 cut(s) | 862, 915, 1148, 1573, 1968 |
| SpeI | ACTAGT | 2 cut(s) | 64, 587 |
| SseBI | AGGCCT | 1 cut(s) | 1908 |
| SsiI | CCGC | 1 cut(s) | 1039 |
| SspI | AATATT | 2 cut(s) | 277, 691 |
| SspMI | CTAG | 5 cut(s) | 65, 588, 812, 963, 1887 |
| SstI | GAGCTC | 2 cut(s) | 1603, 1857 |
| StuI | AGGCCT | 1 cut(s) | 1908 |
| StyD4I | CCNGG | 2 cut(s) | 467, 2195 |
| StyI | CCWWGG | 3 cut(s) | 1297, 1306, 2171 |
| TaaI | ACNGT | 6 cut(s) | 39, 215, 556, 1315, 1646, 2008 |
| TaiI | ACGT | 1 cut(s) | 1827 |
| TaqI | TCGA | 3 cut(s) | 1851, 1940, 1969 |
| TaqII | GACCGA | 1 cut(s) | 1487 |
| TatI | WGTACW | 4 cut(s) | 53, 1113, 1756, 1788 |
| TfiI | GAWTC | 4 cut(s) | 491, 857, 2092, 2234 |
| TscAI | CASTG | 8 cut(s) | 232, 337, 409, 709, 1075, 1938, 2109, 2224 |
| TseFI | GTSAC | 5 cut(s) | 215, 653, 806, 1820, 1890 |
| TseI | GCWGC | 4 cut(s) | 546, 1192, 1622, 1625 |
| Tsp45I | GTSAC | 5 cut(s) | 215, 653, 806, 1820, 1890 |
| TspRI | CASTG | 8 cut(s) | 232, 337, 409, 709, 1075, 1938, 2109, 2224 |
| Tth111I | GACNNNGTC | 2 cut(s) | 716, 1888 |
| Vha464I | CTTAAG | 2 cut(s) | 862, 1573 |
| VpaK11BI | GGWCC | 4 cut(s) | 477, 712, 771, 1843 |
| VspI | ATTAAT | 1 cut(s) | 1401 |
| XapI | RAATTY | 2 cut(s) | 679, 1496 |
| XceI | RCATGY | 1 cut(s) | 400 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 706 |
| XhoI | CTCGAG | 1 cut(s) | 1968 |
| XmiI | GTMKAC | 2 cut(s) | 210, 1658 |
| XmnI | GAANNNNTTC | 3 cut(s) | 398, 1157, 1548 |
| XspI | CTAG | 5 cut(s) | 65, 588, 812, 963, 1887 |
| ZrmI | AGTACT | 2 cut(s) | 55, 1790 |
| Zsp2I | ATGCAT | 3 cut(s) | 640, 793, 926 |