Rh2DG521000

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000052
Physical Location & Seq
Reverse (-)
74543127 .. 74546071
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG521000.1

Sequence Viewer

Length: 2223 bp
ATGCAATCCCTAGTTCATCATATGATATCTATTATGGTTTTATGTTTATGGAGCTTGAGTACTACACCAGCAGTAGCAGCAGAATCAGGAACAGCGCTGCCGATAGCAAAGCCGAATTGCCAAGAGAAATGTGGAAATGTCAGCATCCCGTACCCTTTCGGGATCGGGCCTGATAAAGATTGCTACCTTGAAGAGTGGTTTGAAATAGACTGCAACCAGTCTACTAGGCACAAGCCTTTCTTGAGGCTTACACAACAGGAGGTACTGAGCATTTCTATCAAAGACGGCACGCTTCAGGTGAACAGCCCCGTTACTTTCTTCTGCAACGTAACAGGAAGTAGCCAGCCCGCAAATCTGACCGGAAGCCCTTTCGTGTACTCTCAGAGACAAAACAGATTCACCGCAGCGACTTGCGGCTTTGTCTCCTTGGTGAGCTCAGATCAATCGGTGGTTGGTGGGTGCAGGTCAATTTGTGATAAGCAGAATAGCGGTAGATACTCTGATGGCTGTGATATTGGTACTAATTGTTGCCAGACGAGTATTCCTCCGTATCTCAGGGTTGTCACTGCTAGCGTAATGGTGGATGGACCTGCCAACATGACCGGCCGAAATGACAGTGGAAAGCCGGATTGTAGTGACTACGCATTCCTGGTTGATAAAGATTGGTTTGAGACTGCATCAAACTATAGCGCCATTAGGAATATGCTCCATGTTCCTGTGGCACTAGAATGGAGTGTAATCAATGACACTAATTCGTCGTCTGCATTTCAAGGAAATAAGAAATGCGATTTTGATACTTCTTTGGCACTTAATCGGTCAGTGCTTTCTTGCCGCTGCCCACAAGGGTTCGATGGAAATCCTTATCTTCTCCAACCTTGTCAAGACATTGATGAGTGCATAGATGGTACTAAACGATGCTGGCCCGGCAGCAAGTGCATGAACCTTCCGGGTTCGTTCAAATGCGAGGACGAAAAGGATACAATTAAATTGGCACTTGCAGGTCTTGGCTCGAGTCTTGGACTGTTATTATTACTTATTGCTGCATGGTGGGTACACAAACTGGTGAAGAAAAGGAAAAGCATTAAACGCAAGGAAATGTCATTCAAAAGAAACGGAGGTTTGTTGTTAGAAAGACAGTTATCATCTGGTGAAGTTAATCTTGAGAAAATTAAGTTGTTCAAATCAAAAGAGCTGGAGAAGTCCACTGATAATTTTCACGTTGATAGAATCCTTGGCAAAGGTGGCCAAGGGACTGTCTACAAAGGTATGCTAACAGATGGAAGAATCGTTGCAGTAAAAAGGTCTACAAAAGTGGACGAAGGTAGACTTTCACAGTTTATAAATGAGGTTGTTATTCTTTCACAAATCAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACAGAGGTTCCTCTCTTGGTCTATGAGTTTATTCCGAATGGATCCCTTTCCCAATATATCCATGAGCAGCACGAGGATTTTCCACTTACGTGGGAATTGCGCTTAAGGATTGCCACGGAAATTGCAGGAGCCCTTTCCTACTTACACGGTGCAGCTTCAATTCCTATATTTCATAGAGACATCAAGTCGACAAATATATTGCTAGATGAAAAATACAGGGCAAAAATTGCCGACTTTGGAACTTCTAGATCAGTTGCCATAGACCAAACTCACTTGACCACACTTGTATATGGGACATTTGGTTACTTGGACCCAGAATACTTCCAGTCGAGCCAATTTACGGATAAAAGTGATGTGTATAGCTTTGGAGTGGTCCTTGTTGAACTCTTGAGTGGACAAAAACCAATCTCTGTGACAAGATCACAAGAAGAAGGTCGGAGTCTTGCAACACATTTCATTATTTTGATGCAGGATGATCGGCTATTTGAAGTTGTTGATGCTCGAGTTTTGAATGAAGCCTCTAAAGAAGATATCTTACTAGTTGCCAACCTTGCAAGAAGATGTTTGCATTTGAATGGTAGGAACCGCCCCACAATGAGAGAGGTAACAGCAGAGCTGGAGGGGATTCAAATGTCACAAAAAACAACTAATGGTGAGCAAAATTATGAAGAGGTTGATATTGTCAGGTATGATTCAATTGAGCCATGGGATGTTGCTTCAACTTCAACAGGAACAGTAACAGGAACAAGGTCAGGTGTTGCTGCTGGTCCGCCTTCATCACCATCGCAAGGAATTGCACTGTTATCTCTCTAG

Protein Analysis

740

Amino Acids

81.77

Weight (kDa)

6.11

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 101 1.2e-13 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 322 2.7e-06 Calcium-binding EGF domain
Pkinase PF00069 405 - 673 1.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 406 - 676 1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
Acc36I ACCTGC 3 cut(s) 453, 598, 989
AccI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
AciI CCGC 7 cut(s) 348, 402, 414, 489, 832, 1996, 2180
AclWI GGATC 3 cut(s) 170, 1446, 1459
AcoI YGGCCR 2 cut(s) 604, 1243
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 7 cut(s) 61, 152, 264, 377, 520, 907, 1053
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 2 cut(s) 1750, 2198
AflII CTTAAG 1 cut(s) 1513
AhdI GACNNNNNGTC 1 cut(s) 1594
AhlI ACTAGT 1 cut(s) 1948
AjnI CCWGG 1 cut(s) 648
AleI CACNNNNGTG 1 cut(s) 1498
AluBI AGCT 6 cut(s) 54, 435, 1192, 1565, 1773, 2026
AluI AGCT 6 cut(s) 54, 435, 1192, 1565, 1773, 2026
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
AlwI GGATC 3 cut(s) 170, 1446, 1459
Ama87I CYCGRG 2 cut(s) 1009, 1911
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 4 cut(s) 167, 604, 920, 1243
ApeKI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1477, 1562, 2171
AspLEI GCGC 3 cut(s) 97, 692, 1512
AspS9I GGNCC 6 cut(s) 167, 587, 921, 1720, 1783, 2177
AsuC2I CCSGG 2 cut(s) 924, 948
AsuHPI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2075, 2181
AsuNHI GCTAGC 1 cut(s) 569
AvaI CYCGRG 2 cut(s) 1009, 1911
AvaII GGWCC 4 cut(s) 587, 1720, 1783, 2177
BalI TGGCCA 1 cut(s) 1245
BamHI GGATCC 1 cut(s) 1451
BanII GRGCYC 2 cut(s) 437, 1543
BarI GAAGNNNNNNTAC 2 cut(s) 1929, 1961
BauI CACGAG 1 cut(s) 1481
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BccI CCATC 6 cut(s) 497, 578, 845, 896, 1271, 2200
BceAI ACGGC 1 cut(s) 301
BcgI CGANNNNNNTGC 2 cut(s) 1868, 1902
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 1 cut(s) 970
BcnI CCSGG 2 cut(s) 924, 948
BcoDI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BcuI ACTAGT 1 cut(s) 1948
BfaI CTAG 8 cut(s) 11, 225, 570, 725, 1613, 1656, 1949, 2221
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 2 cut(s) 98, 693
BfrI CTTAAG 1 cut(s) 1513
BfuAI ACCTGC 3 cut(s) 453, 598, 989
BfuI GTATCC 1 cut(s) 970
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 3 cut(s) 650, 924, 948
Bme18I GGWCC 4 cut(s) 587, 1720, 1783, 2177
BmeRI GACNNNNNGTC 1 cut(s) 1594
BmeT110I CYCGRG 2 cut(s) 1009, 1911
BmgT120I GGNCC 6 cut(s) 167, 587, 921, 1720, 1783, 2177
BmiI GGNNCC 5 cut(s) 1419, 1453, 1540, 1722, 1994
BmrFI CCNGG 3 cut(s) 650, 924, 948
BmsI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
BmtI GCTAGC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpmI CTGGAG 2 cut(s) 1214, 2048
BpuEI CTTGAG 4 cut(s) 76, 262, 1181, 1819
BpuMI CCSGG 2 cut(s) 924, 948
BsaAI YACGTR 1 cut(s) 1500
BsaBI GATNNNNATC 1 cut(s) 855
BsaJI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 2 cut(s) 1750, 2198
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 3 cut(s) 217, 1065, 1735
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BseGI GGATG 4 cut(s) 144, 589, 1888, 2125
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 2 cut(s) 1750, 2198
BseMII CTCAG 4 cut(s) 257, 395, 450, 568
BseNI ACTGG 3 cut(s) 217, 1065, 1735
BseX3I CGGCCG 1 cut(s) 604
BseXI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BsgI GTGCAG 2 cut(s) 481, 1581
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 4 cut(s) 169, 606, 922, 1245
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 2 cut(s) 1009, 1911
BsiSI CCGG 5 cut(s) 360, 603, 626, 924, 947
BslFI GGGAC 2 cut(s) 1264, 1717
BslI CCNNNNNNNGG 2 cut(s) 1750, 2198
BsmAI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BsmFI GGGAC 2 cut(s) 1264, 1717
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 169, 606, 922, 1245
BsoBI CYCGRG 2 cut(s) 1009, 1911
Bsp1286I GDGCHC 2 cut(s) 437, 1543
Bsp143I GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
Bsp19I CCATGG 1 cut(s) 2114
BspACI CCGC 7 cut(s) 348, 402, 414, 489, 832, 1996, 2180
BspANI GGCC 4 cut(s) 169, 606, 922, 1245
BspCNI CTCAG 4 cut(s) 258, 394, 449, 567
BspLI GGNNCC 5 cut(s) 1419, 1453, 1540, 1722, 1994
BspMI ACCTGC 3 cut(s) 453, 598, 989
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 3 cut(s) 170, 1446, 1459
BspTI CTTAAG 1 cut(s) 1513
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 3 cut(s) 217, 1065, 1735
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BssMI GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
BssSI CACGAG 1 cut(s) 1481
BssT1I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
Bst2BI CACGAG 1 cut(s) 1481
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
Bst6I CTCTTC 2 cut(s) 186, 2073
BstAFI CTTAAG 1 cut(s) 1513
BstAPI GCANNNNNTGC 2 cut(s) 933, 1637
BstBAI YACGTR 1 cut(s) 1500
BstC8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
BstDEI CTNAG 4 cut(s) 266, 381, 436, 554
BstDSI CCRYGG 2 cut(s) 1524, 2114
BstF5I GGATG 4 cut(s) 144, 589, 1888, 2125
BstH2I RGCGCY 2 cut(s) 98, 693
BstHHI GCGC 3 cut(s) 97, 692, 1512
BstKTI GATC 6 cut(s) 165, 442, 1454, 1661, 1832, 1888
BstMAI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BstMBI GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 648, 922, 946
BstSFI CTRYAG 1 cut(s) 685
BstV1I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BstX2I RGATCY 1 cut(s) 1451
BstXI CCANNNNNNTGG 1 cut(s) 1500
BstYI RGATCY 1 cut(s) 1451
BstZI CGGCCG 1 cut(s) 604
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 4 cut(s) 169, 606, 922, 1245
BtgI CCRYGG 2 cut(s) 1524, 2114
BtgZI GCGATG 1 cut(s) 2178
BtsCI GGATG 4 cut(s) 144, 589, 1888, 2125
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 5 cut(s) 564, 622, 825, 1203, 2207
BveI ACCTGC 3 cut(s) 453, 598, 989
Cac8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
CfoI GCGC 3 cut(s) 97, 692, 1512
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 6 cut(s) 167, 587, 921, 1720, 1783, 2177
Csp6I GTAC 7 cut(s) 60, 151, 263, 376, 519, 906, 1052
CspCI CAANNNNNGTGG 2 cut(s) 1513, 1548
CviAII CATG 6 cut(s) 598, 710, 937, 1044, 1472, 2115
CviQI GTAC 7 cut(s) 60, 151, 263, 376, 519, 906, 1052
DdeI CTNAG 4 cut(s) 266, 381, 436, 554
DpnI GATC 6 cut(s) 164, 441, 1453, 1660, 1831, 1887
DpnII GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
DriI GACNNNNNGTC 1 cut(s) 1594
EaeI YGGCCR 2 cut(s) 604, 1243
EagI CGGCCG 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 186, 2073
Eam1105I GACNNNNNGTC 1 cut(s) 1594
EarI CTCTTC 2 cut(s) 186, 2073
EciI GGCGGA 1 cut(s) 2169
Ecl136II GAGCTC 1 cut(s) 435
EclXI CGGCCG 1 cut(s) 604
Eco130I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
Eco24I GRGCYC 2 cut(s) 437, 1543
Eco32I GATATC 2 cut(s) 27, 1942
Eco47I GGWCC 4 cut(s) 587, 1720, 1783, 2177
Eco47III AGCGCT 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 1009, 1911
EcoICRI GAGCTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 2 cut(s) 27, 1942
EcoT14I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
EcoT38I GRGCYC 2 cut(s) 437, 1543
ErhI CCWWGG 4 cut(s) 426, 1231, 1246, 2114
FaeI CATG 6 cut(s) 601, 713, 940, 1047, 1475, 2118
FalI AAGNNNNNCTT 6 cut(s) 224, 256, 1143, 1175, 1311, 1343
FaqI GGGAC 2 cut(s) 1264, 1717
FatI CATG 6 cut(s) 597, 709, 936, 1043, 1471, 2114
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
FokI GGATG 4 cut(s) 131, 596, 1895, 2132
FriOI GRGCYC 2 cut(s) 437, 1543
FspBI CTAG 8 cut(s) 11, 225, 570, 725, 1613, 1656, 1949, 2221
GlaI GCGC 3 cut(s) 96, 691, 1511
GsuI CTGGAG 2 cut(s) 1214, 2048
HaeII RGCGCY 2 cut(s) 98, 693
HaeIII GGCC 4 cut(s) 169, 606, 922, 1245
HapII CCGG 5 cut(s) 360, 603, 626, 924, 947
HhaI GCGC 3 cut(s) 97, 692, 1512
Hin1II CATG 6 cut(s) 601, 713, 940, 1047, 1475, 2118
Hin6I GCGC 3 cut(s) 95, 690, 1510
HinP1I GCGC 3 cut(s) 95, 690, 1510
HincII GTYRAC 1 cut(s) 1599
HindII GTYRAC 1 cut(s) 1599
HinfI GANTC 8 cut(s) 83, 396, 1012, 1227, 1284, 1849, 2035, 2102
HpaII CCGG 5 cut(s) 360, 603, 626, 924, 947
HphI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2075, 2181
Hpy188I TCNGA 6 cut(s) 357, 384, 439, 502, 1446, 1848
Hpy188III TCNNGA 7 cut(s) 87, 160, 241, 881, 1160, 1656, 1798
Hpy99I CGWCG 1 cut(s) 760
HpyAV CCTTC 4 cut(s) 953, 1313, 1835, 2193
HpyCH4III ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
HpyCH4IV ACGT 3 cut(s) 327, 1218, 1499
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
HpyF3I CTNAG 4 cut(s) 266, 381, 436, 554
HpySE526I ACGT 3 cut(s) 327, 1218, 1499
Hsp92II CATG 6 cut(s) 601, 713, 940, 1047, 1475, 2118
HspAI GCGC 3 cut(s) 95, 690, 1510
Kzo9I GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
LmnI GCTCC 3 cut(s) 51, 711, 1538
Lsp1109I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
LweI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
MaeI CTAG 8 cut(s) 11, 225, 570, 725, 1613, 1656, 1949, 2221
MaeII ACGT 3 cut(s) 327, 1218, 1499
MaeIII GTNAC 9 cut(s) 310, 328, 562, 635, 1712, 1822, 2014, 2043, 2146
MalI GATC 6 cut(s) 164, 441, 1453, 1660, 1831, 1887
MboI GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
MboII GAAGA 9 cut(s) 203, 310, 857, 1078, 1293, 1850, 1949, 1980, 2090
MfeI CAATTG 1 cut(s) 2106
MflI RGATCY 1 cut(s) 1451
MhlI GDGCHC 2 cut(s) 437, 1543
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 2 cut(s) 1021, 1858
MmeI TCCRAC 2 cut(s) 895, 1826
Mox20I TGGCCA 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
MslI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 1 cut(s) 834
MspCI CTTAAG 1 cut(s) 1513
MspI CCGG 5 cut(s) 360, 603, 626, 924, 947
MspR9I CCNGG 3 cut(s) 650, 924, 948
MunI CAATTG 1 cut(s) 2106
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
NciI CCSGG 2 cut(s) 924, 948
NcoI CCATGG 1 cut(s) 2114
NdeI CATATG 1 cut(s) 21
NdeII GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 6 cut(s) 601, 713, 940, 1047, 1475, 2118
NlaIV GGNNCC 5 cut(s) 1419, 1453, 1540, 1722, 1994
NmuCI GTSAC 4 cut(s) 562, 635, 1822, 2043
OliI CACNNNNGTG 1 cut(s) 1498
PaeR7I CTCGAG 2 cut(s) 1009, 1911
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 6 cut(s) 83, 396, 1227, 1284, 2035, 2102
PleI GAGTC 2 cut(s) 1020, 1857
PpsI GAGTC 2 cut(s) 1020, 1857
Ppu21I YACGTR 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 1340
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 5 cut(s) 1419, 1453, 1540, 1722, 1994
PspPI GGNCC 6 cut(s) 167, 587, 921, 1720, 1783, 2177
PspXI VCTCGAGB 2 cut(s) 1009, 1911
PsuI RGATCY 1 cut(s) 1451
RsaI GTAC 7 cut(s) 61, 152, 264, 377, 520, 907, 1053
RsaNI GTAC 7 cut(s) 60, 151, 263, 376, 519, 906, 1052
RseI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
SacI GAGCTC 1 cut(s) 437
SalI GTCGAC 1 cut(s) 1597
SaqAI TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
Sau3AI GATC 6 cut(s) 162, 439, 1451, 1658, 1829, 1885
Sau96I GGNCC 6 cut(s) 167, 587, 921, 1720, 1783, 2177
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 2 cut(s) 1021, 1858
ScrFI CCNGG 3 cut(s) 650, 924, 948
SduI GDGCHC 2 cut(s) 437, 1543
SfaNI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
SfcI CTRYAG 1 cut(s) 685
Sfr274I CTCGAG 2 cut(s) 1009, 1911
SinI GGWCC 4 cut(s) 587, 1720, 1783, 2177
SlaI CTCGAG 2 cut(s) 1009, 1911
SmiMI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
SmlI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1513, 1798, 1911
SmoI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1513, 1798, 1911
SpeI ACTAGT 1 cut(s) 1948
SsiI CCGC 7 cut(s) 348, 402, 414, 489, 832, 1996, 2180
SspMI CTAG 8 cut(s) 11, 225, 570, 725, 1613, 1656, 1949, 2221
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 648, 922, 946
StyI CCWWGG 4 cut(s) 426, 1231, 1246, 2114
TaaI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
TaiI ACGT 3 cut(s) 330, 1221, 1502
TaqI TCGA 5 cut(s) 849, 1010, 1598, 1739, 1912
TaqII GACCGA 1 cut(s) 804
TatI WGTACW 2 cut(s) 59, 375
TauI GCSGC 2 cut(s) 417, 834
TfiI GAWTC 6 cut(s) 83, 396, 1227, 1284, 2035, 2102
Tru1I TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
Tru9I TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
TscAI CASTG 5 cut(s) 571, 622, 825, 1210, 2214
TseFI GTSAC 4 cut(s) 562, 635, 1822, 2043
TseI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1477, 1562, 2171
Tsp45I GTSAC 4 cut(s) 562, 635, 1822, 2043
TspDTI ATGAA 8 cut(s) 5, 953, 1571, 1632, 1855, 1938, 2091, 2175
TspGWI ACGGA 4 cut(s) 537, 1128, 1541, 1766
TspRI CASTG 5 cut(s) 571, 622, 825, 1210, 2214
Vha464I CTTAAG 1 cut(s) 1513
VpaK11BI GGWCC 4 cut(s) 587, 1720, 1783, 2177
XbaI TCTAGA 1 cut(s) 1655
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XhoI CTCGAG 2 cut(s) 1009, 1911
XmiI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
XspI CTAG 8 cut(s) 11, 225, 570, 725, 1613, 1656, 1949, 2221
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.