RLG00000020648

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000052
Physical Location & Seq
Reverse (-)
67112845 .. 67115838
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020648

Sequence Viewer

Length: 2289 bp
ATGGTTCCCCAGTTGCTATTTCATATTACTCTGTTACTGTGGTCTATGAGTAGTACTGAAGTACTAGTTGTAGCATCAGAAGCACCCATTGTAGCATCAAAAGCACCCATAGCAAAGCCTAATTGTCCATCGCATTGTGGAGGCATTGAAATCCCATACCCTTTTGGGATAGGAGCAGGTTGTTACATTAATGACGACTGGTTCCAAGTATTCTGTGACAACTCTACTGGCTCTCCTACACCTTTCTTGAACGCCACCAAACTGGAGGTACTAGATATTTCTGTTGAAGGCACTCTAAAGGTCAGAAACCCAATCACCTTCTCTAATTGCAGTGATAAGCCAAATCGTCAAGCAGTCAACTTGGAGGGAAGCCCTTTTATGTTCTCCCAGAAGAACATGTTCACTGCAGTAGGTTGCGGAGTTATGGCCACAGTCACCTCAAACTCAAATGGCGTTACCATTTCAGCCGGGTGCAGGTCCGAATGTTATGATTCTACTGATAGTAAAAATAGTAGCAGCCATAATACCTGCAATGGTGTGGATTGCTGCCAGACTGTCATCCCTTCTTCCCTCTCTGCCTTCAATACTAGTTTCCAGCCGGCTAATGATAATTCCACAGAAAGTTCATGTAATTATGCATTTTTGGTTGATGGTGATTGGTTTGAGTCATTTGCCAAAAATTCAACAAATATTTCTGCCACCAGTGATATGGACCAAGTCCCCGTGGAGCTAAAATGGAACTTGTACCATTCAACAACTGATGTTTTTGGGACCTTTAAAGAAACAAATGCATCGGTGCGGTATGCTAATGGCCCAGATGGTCCGTGCGAGACTTACAACGACAGCTCTTCATCATATGAATCCTTAAGGCTTGAATGTTCCTGTGGATGGGGCTTTACGGGAAATCCATATCTTCCTCAAGGATGCATAGATATCAATGAATGTGAGGATCCCGAAAAAAAAGCTGAATGTTCTCCAGGGTACGAATGTGACAATTTTTTTGGTGGATATGGATGCTCCCTCCCAGCTGAAACCAGCAAGTCACGGGTTAAAGTAATCTTTATAGGTATCGGCAGTGGTCTTGGACTGATAGTTCTACTAATTGGTGCATGGTGGTTGTACAAACTTTGGAAGAAAAGGAAAAACATTAAACTCAAGAAGAAGTTCTTTAAACAAAATGGGGGACTATTATTAGAGCAGCAACTGTCTTCCGGTGAAGTTAATGTTGAGAAAATTAAGCTGTTCAATTCAAAGGAATTAGAAAAAGCCACAGACCGGTTTAATGTAGATAGAATACTTGGCCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGGCAGATGGAAGAATTGTTGCTGTAAAGAAGTCTAAAATAGTTGATGGGGGTGAAGTGGGACAATTCATTAATGAAATTGTCATTCTTTCACAAATTAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACCGAAGTTCCACTTTTGGTTTACGAATTCATACCCAAAGGGACTATCTACCAGTATCTCCATGATCAGAATGAAGAGTTTCCACTCACATGGGAAATGCGCTTAAGGATTTCTGCAGAAGTTGCCGGAGCTCTTTCATACTTACACTCTGCAGCTGCTTTCCCAATTTACCACCGTGATATCAAGTCTACAAACATACTATTAGATGATAAATACAGAGCAAAAGTTGCAGACTTTGGGACTTCAAGATCAGTTTCCATAGACCAAACACACCTAACCACATTTGTACATGGTACATTTGGTTACTTGGATCCGGAGTACTTCCAATCAAGTCAATTTACAGACAAAAGTGACGTGTATAGCTTTGGAGTGGTCCTTGTCGAGCTCTTGACTGGAGAAAAACCAGTTTCTCTGACTAGGTCACAAGAAGCCAGAGGCCTTGTTTCATATTTCAATCTCTCACTGGAGTCGAATAATTTGTTTGATATTATTGATGCTCGAGTTAAGGTGGATGGTGTAACAGGAGATATTTTGACAGTAGCTGATCTTGCAAAAAGATGTCTGGATATGAATGGCAAGAGACGGCCTACTATGAAAGAAGTTGCAATGGAGTTGGAGGTGATTCATAAATCAGTAAAATCTTCAAATATACAAGAAAATCATGAAGAGGTTGTGTATGTCCGAAATGAAGTAACTTGTCCTTGGGATGTTGCTTCTACATTAACCGGGTCAGCTTTGGATGGTGGCACTGCTTCATCGATTGATTCACTACCACTAATGTCTAACTCATTGATCACATTCCACCTATCCTAG

Protein Analysis

763

Amino Acids

83.75

Weight (kDa)

5.24

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 6.2e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 427 - 694 7.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 429 - 697 2.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 167, 465, 536
AccI GTMKAC 1 cut(s) 1664
AccIII TCCGGA 1 cut(s) 1789
AciI CCGC 2 cut(s) 417, 799
AclWI GGATC 4 cut(s) 944, 957, 1781, 1794
AcoI YGGCCR 2 cut(s) 426, 1300
AcsI RAATTY 2 cut(s) 679, 1502
AcuI CTGAAG 1 cut(s) 78
AfiI CCNNNNNNNGG 3 cut(s) 474, 888, 1275
AflII CTTAAG 2 cut(s) 865, 1579
AflIII ACRYGT 2 cut(s) 396, 1830
AgeI ACCGGT 1 cut(s) 1275
AhlI ACTAGT 2 cut(s) 64, 587
AjiI CACGTC 1 cut(s) 1831
AjnI CCWGG 1 cut(s) 976
AloI GAACNNNNNNTCC 2 cut(s) 1077, 1109
Alw21I GWGCWC 2 cut(s) 1609, 1863
Alw26I GTCTC 3 cut(s) 824, 1469, 2050
AlwI GGATC 4 cut(s) 944, 957, 1781, 1794
AlwNI CAGNNNCTG 2 cut(s) 1204, 2018
Ama87I CYCGRG 1 cut(s) 1974
Aor13HI TCCGGA 1 cut(s) 1789
AoxI GGCC 6 cut(s) 426, 811, 1300, 1309, 1912, 2060
ApeKI GCWGC 5 cut(s) 516, 546, 1198, 1628, 1631
ApoI RAATTY 2 cut(s) 679, 1502
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
AseI ATTAAT 2 cut(s) 189, 1407
AsiGI ACCGGT 1 cut(s) 1275
Asp700I GAANNNNTTC 3 cut(s) 398, 1163, 1554
AspLEI GCGC 1 cut(s) 1578
AspS9I GGNCC 6 cut(s) 477, 712, 771, 812, 821, 1849
AsuC2I CCSGG 2 cut(s) 469, 2203
AsuHPI GGTGA 6 cut(s) 307, 427, 665, 1226, 1400, 2107
AvaI CYCGRG 1 cut(s) 1974
AvaII GGWCC 5 cut(s) 477, 712, 771, 821, 1849
BaeI ACNNNNGTAYC 2 cut(s) 973, 1006
BalI TGGCCA 2 cut(s) 428, 1302
BamHI GGATCC 2 cut(s) 949, 1786
BanII GRGCYC 2 cut(s) 1609, 1863
BbsI GAAGAC 1 cut(s) 1200
Bbv12I GWGCWC 2 cut(s) 1609, 1863
BbvI GCAGC 5 cut(s) 528, 533, 1210, 1618, 1640
BccI CCATC 8 cut(s) 136, 644, 812, 882, 1337, 1376, 1982, 2210
BceAI ACGGC 1 cut(s) 2075
BciT130I CCWGG 1 cut(s) 978
BclI TGATCA 2 cut(s) 1540, 2268
BcnI CCSGG 2 cut(s) 469, 2203
BcoDI GTCTC 3 cut(s) 824, 1469, 2050
BcuI ACTAGT 2 cut(s) 64, 587
BfaI CTAG 5 cut(s) 65, 272, 588, 1893, 2287
BfmI CTRYAG 3 cut(s) 405, 1590, 1626
BfrI CTTAAG 2 cut(s) 865, 1579
BfuAI ACCTGC 3 cut(s) 167, 465, 536
BglI GCCNNNNNGGC 1 cut(s) 1308
BisI GCNGC 5 cut(s) 517, 547, 1199, 1629, 1632
BlsI GCNGC 5 cut(s) 518, 548, 1200, 1630, 1633
BmcAI AGTACT 3 cut(s) 55, 63, 1796
Bme1390I CCNGG 3 cut(s) 469, 978, 2203
Bme18I GGWCC 5 cut(s) 477, 712, 771, 821, 1849
BmeT110I CYCGRG 1 cut(s) 1974
BmgBI CACGTC 1 cut(s) 1831
BmgT120I GGNCC 6 cut(s) 477, 712, 771, 812, 821, 1849
BmiI GGNNCC 5 cut(s) 6, 203, 772, 951, 1788
BmrFI CCNGG 3 cut(s) 469, 978, 2203
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 6 cut(s) 83, 104, 800, 914, 1004, 1960
BmuI ACTGGG 1 cut(s) 4
BpiI GAAGAC 1 cut(s) 1200
BpmI CTGGAG 4 cut(s) 284, 960, 1890, 1961
BpuEI CTTGAG 2 cut(s) 903, 1139
BpuMI CCSGG 2 cut(s) 469, 2203
Bsa29I ATCGAT 1 cut(s) 2234
BsaI GGTCTC 1 cut(s) 1469
BsaJI CCNNGG 5 cut(s) 723, 977, 1303, 1312, 2177
BsaWI WCCGGW 3 cut(s) 1211, 1275, 1789
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 888, 1275
Bse118I RCCGGY 2 cut(s) 598, 1275
Bse1I ACTGG 9 cut(s) 10, 203, 232, 267, 702, 1528, 1873, 1880, 1944
Bse3DI GCAATG 2 cut(s) 538, 2088
BseAI TCCGGA 1 cut(s) 1789
BseBI CCWGG 1 cut(s) 978
BseCI ATCGAT 1 cut(s) 2234
BseDI CCNNGG 5 cut(s) 723, 977, 1303, 1312, 2177
BseGI GGATG 7 cut(s) 558, 893, 929, 1019, 1993, 2188, 2221
BseLI CCNNNNNNNGG 3 cut(s) 474, 888, 1275
BseMI GCAATG 2 cut(s) 538, 2088
BseNI ACTGG 9 cut(s) 10, 203, 232, 267, 702, 1528, 1873, 1880, 1944
BseXI GCAGC 5 cut(s) 528, 533, 1210, 1618, 1640
BseYI CCCAGC 1 cut(s) 1024
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 6 cut(s) 428, 813, 1302, 1311, 1914, 2062
BshTI ACCGGT 1 cut(s) 1275
BshVI ATCGAT 1 cut(s) 2234
BsiHKAI GWGCWC 2 cut(s) 1609, 1863
BsiHKCI CYCGRG 1 cut(s) 1974
BsiSI CCGG 7 cut(s) 468, 599, 1212, 1276, 1602, 1790, 2202
BslFI GGGAC 6 cut(s) 704, 784, 1197, 1410, 1531, 1729
BslI CCNNNNNNNGG 3 cut(s) 474, 888, 1275
BsmAI GTCTC 3 cut(s) 824, 1469, 2050
BsmBI CGTCTC 1 cut(s) 2050
BsmFI GGGAC 6 cut(s) 704, 784, 1197, 1410, 1531, 1729
BsnI GGCC 6 cut(s) 428, 813, 1302, 1311, 1914, 2062
Bso31I GGTCTC 1 cut(s) 1469
BsoBI CYCGRG 1 cut(s) 1974
Bsp1286I GDGCHC 2 cut(s) 1609, 1863
Bsp13I TCCGGA 1 cut(s) 1789
Bsp1407I TGTACA 2 cut(s) 1119, 1762
Bsp143I GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
BspACI CCGC 2 cut(s) 417, 799
BspANI GGCC 6 cut(s) 428, 813, 1302, 1311, 1914, 2062
BspDI ATCGAT 1 cut(s) 2234
BspEI TCCGGA 1 cut(s) 1789
BspHI TCATGA 1 cut(s) 2137
BspLI GGNNCC 5 cut(s) 6, 203, 772, 951, 1788
BspMAI CTGCAG 3 cut(s) 409, 1594, 1630
BspMI ACCTGC 3 cut(s) 167, 465, 536
BspPI GGATC 4 cut(s) 944, 957, 1781, 1794
BspQI GCTCTTC 1 cut(s) 853
BspTI CTTAAG 2 cut(s) 865, 1579
BspTNI GGTCTC 1 cut(s) 1469
BsrDI GCAATG 2 cut(s) 538, 2088
BsrFI RCCGGY 2 cut(s) 598, 1275
BsrGI TGTACA 2 cut(s) 1119, 1762
BsrI ACTGG 9 cut(s) 10, 203, 232, 267, 702, 1528, 1873, 1880, 1944
BssAI RCCGGY 2 cut(s) 598, 1275
BssECI CCNNGG 5 cut(s) 723, 977, 1303, 1312, 2177
BssMI GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
BssT1I CCWWGG 3 cut(s) 1303, 1312, 2177
Bst2UI CCWGG 1 cut(s) 978
Bst4CI ACNGT 7 cut(s) 39, 433, 556, 1206, 1321, 1652, 2014
Bst6I CTCTTC 3 cut(s) 853, 1545, 2136
BstAFI CTTAAG 2 cut(s) 865, 1579
BstAPI GCANNNNNTGC 2 cut(s) 1598, 1703
BstAUI TGTACA 2 cut(s) 1119, 1762
BstC8I GCNNGC 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 7 cut(s) 558, 893, 929, 1019, 1993, 2188, 2221
BstHHI GCGC 1 cut(s) 1578
BstKTI GATC 6 cut(s) 952, 1543, 1727, 1789, 2023, 2271
BstMAI GTCTC 3 cut(s) 824, 1469, 2050
BstMBI GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
BstMWI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1308, 1598, 1703, 2024
BstNI CCWGG 1 cut(s) 978
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 467, 976, 2201
BstSFI CTRYAG 3 cut(s) 405, 1590, 1626
BstV1I GCAGC 5 cut(s) 528, 533, 1210, 1618, 1640
BstV2I GAAGAC 1 cut(s) 1200
BstX2I RGATCY 2 cut(s) 949, 1786
BstXI CCANNNNNNTGG 3 cut(s) 262, 709, 1566
BstYI RGATCY 2 cut(s) 949, 1786
Bsu15I ATCGAT 1 cut(s) 2234
BsuRI GGCC 6 cut(s) 428, 813, 1302, 1311, 1914, 2062
BsuTUI ATCGAT 1 cut(s) 2234
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1831
BtsCI GGATG 7 cut(s) 558, 893, 929, 1019, 1993, 2188, 2221
BtsI GCAGTG 4 cut(s) 337, 402, 1081, 2223
BtsIMutI CAGTG 6 cut(s) 337, 402, 709, 1081, 1937, 2223
BveI ACCTGC 3 cut(s) 167, 465, 536
Cac8I GCNNGC 1 cut(s) 600
CaiI CAGNNNCTG 2 cut(s) 1204, 2018
CciI TCATGA 1 cut(s) 2137
CfoI GCGC 1 cut(s) 1578
Cfr10I RCCGGY 2 cut(s) 598, 1275
Cfr13I GGNCC 6 cut(s) 477, 712, 771, 812, 821, 1849
ClaI ATCGAT 1 cut(s) 2234
CspAI ACCGGT 1 cut(s) 1275
CviAII CATG 7 cut(s) 397, 627, 1110, 1538, 1566, 1766, 2138
DpnI GATC 6 cut(s) 951, 1542, 1726, 1788, 2022, 2270
DpnII GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
DraI TTTAAA 2 cut(s) 778, 1171
EaeI YGGCCR 2 cut(s) 426, 1300
Eam1104I CTCTTC 3 cut(s) 853, 1545, 2136
EarI CTCTTC 3 cut(s) 853, 1545, 2136
Ecl136II GAGCTC 2 cut(s) 1607, 1861
Eco130I CCWWGG 3 cut(s) 1303, 1312, 2177
Eco147I AGGCCT 1 cut(s) 1914
Eco24I GRGCYC 2 cut(s) 1609, 1863
Eco31I GGTCTC 1 cut(s) 1469
Eco32I GATATC 2 cut(s) 934, 1657
Eco47I GGWCC 5 cut(s) 477, 712, 771, 821, 1849
Eco53kI GAGCTC 2 cut(s) 1607, 1861
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1974
EcoICRI GAGCTC 2 cut(s) 1607, 1861
EcoO109I RGGNCCY 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 1502
EcoRII CCWGG 1 cut(s) 976
EcoRV GATATC 2 cut(s) 934, 1657
EcoT14I CCWWGG 3 cut(s) 1303, 1312, 2177
EcoT22I ATGCAT 3 cut(s) 640, 793, 929
EcoT38I GRGCYC 2 cut(s) 1609, 1863
ErhI CCWWGG 3 cut(s) 1303, 1312, 2177
Esp3I CGTCTC 1 cut(s) 2050
FaeI CATG 7 cut(s) 400, 630, 1113, 1541, 1569, 1769, 2141
FalI AAGNNNNNCTT 4 cut(s) 1151, 1183, 1473, 1505
FaqI GGGAC 6 cut(s) 704, 784, 1197, 1410, 1531, 1729
FatI CATG 7 cut(s) 396, 626, 1109, 1537, 1565, 1765, 2137
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 2 cut(s) 1540, 2268
FblI GTMKAC 1 cut(s) 1664
Fnu4HI GCNGC 5 cut(s) 517, 547, 1199, 1629, 1632
FokI GGATG 7 cut(s) 545, 900, 936, 1026, 2000, 2195, 2228
FriOI GRGCYC 2 cut(s) 1609, 1863
Fsp4HI GCNGC 5 cut(s) 517, 547, 1199, 1629, 1632
FspBI CTAG 5 cut(s) 65, 272, 588, 1893, 2287
GlaI GCGC 1 cut(s) 1577
GluI GCNGC 5 cut(s) 517, 547, 1199, 1629, 1632
GsaI CCCAGC 1 cut(s) 1028
GsuI CTGGAG 4 cut(s) 284, 960, 1890, 1961
HaeIII GGCC 6 cut(s) 428, 813, 1302, 1311, 1914, 2062
HapII CCGG 7 cut(s) 468, 599, 1212, 1276, 1602, 1790, 2202
HhaI GCGC 1 cut(s) 1578
Hin1II CATG 7 cut(s) 400, 630, 1113, 1541, 1569, 1769, 2141
Hin6I GCGC 1 cut(s) 1576
HinP1I GCGC 1 cut(s) 1576
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 6 cut(s) 491, 665, 860, 1943, 2098, 2240
HpaII CCGG 7 cut(s) 468, 599, 1212, 1276, 1602, 1790, 2202
HphI GGTGA 6 cut(s) 307, 427, 665, 1226, 1400, 2107
Hpy166II GTNNAC 5 cut(s) 358, 402, 1324, 1498, 1665
Hpy188I TCNGA 6 cut(s) 79, 305, 481, 1545, 1890, 2159
Hpy188III TCNNGA 8 cut(s) 247, 953, 1156, 1722, 1790, 1864, 2039, 2138
Hpy8I GTNNAC 5 cut(s) 358, 402, 1324, 1498, 1665
HpyAV CCTTC 4 cut(s) 281, 328, 573, 589
HpyCH4III ACNGT 7 cut(s) 39, 433, 556, 1206, 1321, 1652, 2014
HpyCH4IV ACGT 1 cut(s) 1830
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1308, 1598, 1703, 2024
HpySE526I ACGT 1 cut(s) 1830
Hsp92II CATG 7 cut(s) 400, 630, 1113, 1541, 1569, 1769, 2141
HspAI GCGC 1 cut(s) 1576
Kpn2I TCCGGA 1 cut(s) 1789
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Ksp22I TGATCA 2 cut(s) 1540, 2268
Kzo9I GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 4 cut(s) 173, 727, 1022, 1604
Lsp1109I GCAGC 5 cut(s) 528, 533, 1210, 1618, 1640
LweI GCATC 6 cut(s) 83, 104, 800, 914, 1004, 1960
MaeI CTAG 5 cut(s) 65, 272, 588, 1893, 2287
MaeII ACGT 1 cut(s) 1830
MalI GATC 6 cut(s) 951, 1542, 1726, 1788, 2022, 2270
MboI GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
MflI RGATCY 2 cut(s) 949, 1786
MhlI GDGCHC 2 cut(s) 1609, 1863
MlsI TGGCCA 2 cut(s) 428, 1302
MluNI TGGCCA 2 cut(s) 428, 1302
MlyI GAGTC 2 cut(s) 674, 1952
MmeI TCCRAC 1 cut(s) 2070
Mox20I TGGCCA 2 cut(s) 428, 1302
Mph1103I ATGCAT 3 cut(s) 640, 793, 929
MroI TCCGGA 1 cut(s) 1789
MroNI GCCGGC 1 cut(s) 598
MroXI GAANNNNTTC 3 cut(s) 398, 1163, 1554
MscI TGGCCA 2 cut(s) 428, 1302
MslI CAYNNNNRTG 2 cut(s) 1443, 1564
Msp20I TGGCCA 2 cut(s) 428, 1302
MspA1I CMGCKG 2 cut(s) 1028, 1631
MspCI CTTAAG 2 cut(s) 865, 1579
MspI CCGG 7 cut(s) 468, 599, 1212, 1276, 1602, 1790, 2202
MspR9I CCNGG 3 cut(s) 469, 978, 2203
MvaI CCWGG 1 cut(s) 978
MwoI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1308, 1598, 1703, 2024
NaeI GCCGGC 1 cut(s) 600
NciI CCSGG 2 cut(s) 469, 2203
NdeI CATATG 1 cut(s) 856
NdeII GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
NgoMIV GCCGGC 1 cut(s) 598
NlaIII CATG 7 cut(s) 400, 630, 1113, 1541, 1569, 1769, 2141
NlaIV GGNNCC 5 cut(s) 6, 203, 772, 951, 1788
NmuCI GTSAC 6 cut(s) 215, 433, 989, 1041, 1826, 1896
NsiI ATGCAT 3 cut(s) 640, 793, 929
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 1974
PagI TCATGA 1 cut(s) 2137
PceI AGGCCT 1 cut(s) 1914
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 853
PdiI GCCGGC 1 cut(s) 600
PdmI GAANNNNTTC 3 cut(s) 398, 1163, 1554
PfeI GAWTC 4 cut(s) 491, 860, 2098, 2240
PflFI GACNNNGTC 2 cut(s) 716, 1894
PinAI ACCGGT 1 cut(s) 1275
PkrI GCNGC 5 cut(s) 518, 548, 1200, 1630, 1633
PleI GAGTC 2 cut(s) 673, 1951
PpsI GAGTC 2 cut(s) 673, 1951
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 396
PshBI ATTAAT 2 cut(s) 189, 1407
Psp124BI GAGCTC 2 cut(s) 1609, 1863
Psp5II RGGWCCY 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 976
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 1 cut(s) 976
PspN4I GGNNCC 5 cut(s) 6, 203, 772, 951, 1788
PspPI GGNCC 6 cut(s) 477, 712, 771, 812, 821, 1849
PspPPI RGGWCCY 1 cut(s) 771
PspXI VCTCGAGB 1 cut(s) 1974
PstI CTGCAG 3 cut(s) 409, 1594, 1630
PstNI CAGNNNCTG 2 cut(s) 1204, 2018
PsuI RGATCY 2 cut(s) 949, 1786
PsyI GACNNNGTC 2 cut(s) 716, 1894
PvuII CAGCTG 2 cut(s) 1028, 1631
RseI CAYNNNNRTG 2 cut(s) 1443, 1564
SacI GAGCTC 2 cut(s) 1609, 1863
SapI GCTCTTC 1 cut(s) 853
SatI GCNGC 5 cut(s) 517, 547, 1199, 1629, 1632
Sau3AI GATC 6 cut(s) 949, 1540, 1724, 1786, 2020, 2268
Sau96I GGNCC 6 cut(s) 477, 712, 771, 812, 821, 1849
ScaI AGTACT 3 cut(s) 55, 63, 1796
SchI GAGTC 2 cut(s) 674, 1952
ScrFI CCNGG 3 cut(s) 469, 978, 2203
SduI GDGCHC 2 cut(s) 1609, 1863
SfaNI GCATC 6 cut(s) 83, 104, 800, 914, 1004, 1960
SfcI CTRYAG 3 cut(s) 405, 1590, 1626
SfiI GGCCNNNNNGGCC 1 cut(s) 1308
Sfr274I CTCGAG 1 cut(s) 1974
SinI GGWCC 5 cut(s) 477, 712, 771, 821, 1849
SlaI CTCGAG 1 cut(s) 1974
SmiMI CAYNNNNRTG 2 cut(s) 1443, 1564
SmlI CTYRAG 5 cut(s) 865, 918, 1154, 1579, 1974
SmoI CTYRAG 5 cut(s) 865, 918, 1154, 1579, 1974
SpeI ACTAGT 2 cut(s) 64, 587
SseBI AGGCCT 1 cut(s) 1914
SsiI CCGC 2 cut(s) 417, 799
SspI AATATT 1 cut(s) 691
SspMI CTAG 5 cut(s) 65, 272, 588, 1893, 2287
SstI GAGCTC 2 cut(s) 1609, 1863
StuI AGGCCT 1 cut(s) 1914
StyD4I CCNGG 3 cut(s) 467, 976, 2201
StyI CCWWGG 3 cut(s) 1303, 1312, 2177
TaaI ACNGT 7 cut(s) 39, 433, 556, 1206, 1321, 1652, 2014
TaiI ACGT 1 cut(s) 1833
TaqI TCGA 4 cut(s) 1857, 1946, 1975, 2234
TaqII GACCGA 1 cut(s) 1493
TatI WGTACW 5 cut(s) 53, 61, 1119, 1762, 1794
TfiI GAWTC 4 cut(s) 491, 860, 2098, 2240
TscAI CASTG 6 cut(s) 337, 409, 709, 1081, 1944, 2230
TseFI GTSAC 6 cut(s) 215, 433, 989, 1041, 1826, 1896
TseI GCWGC 5 cut(s) 516, 546, 1198, 1628, 1631
Tsp45I GTSAC 6 cut(s) 215, 433, 989, 1041, 1826, 1896
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 6 cut(s) 337, 409, 709, 1081, 1944, 2230
Tth111I GACNNNGTC 2 cut(s) 716, 1894
Vha464I CTTAAG 2 cut(s) 865, 1579
VpaK11BI GGWCC 5 cut(s) 477, 712, 771, 821, 1849
VspI ATTAAT 2 cut(s) 189, 1407
XapI RAATTY 2 cut(s) 679, 1502
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 1974
XmiI GTMKAC 1 cut(s) 1664
XmnI GAANNNNTTC 3 cut(s) 398, 1163, 1554
XspI CTAG 5 cut(s) 65, 272, 588, 1893, 2287
ZrmI AGTACT 3 cut(s) 55, 63, 1796
Zsp2I ATGCAT 3 cut(s) 640, 793, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.