Rw2G041250

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000052
Physical Location & Seq
Forward (+)
67285570 .. 67289868
4299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041250.1

Sequence Viewer

Length: 2208 bp
ATGGTTGCCCAGTTGCTATTTCATATTACTCTGTTACTGTGGTCTATGAGTAGTACTGAAGTACTAGTTGTAGCATCAGAAGCACCCATTGTAGCAACAGAAGCACCCATAGCAAAGCCTAATTGTCCATCGCATTGTGGAGGCATTGAAATCCCATACCCTTTTGGGATAGGAGCAGGTTGTTACATTCATGACGACTGGTTTCAAGTATACTGTGACAACTCCACTGGCTCTCCTACACCTTTCTTGAACGGCACCAATCTGGAGGTACTAGATATTTCTGTTGAAGGCACTCTAAAGGTCAGAAACCCAATCACCTTCTCTAATTGCAGTGATAAGCCAAATCGTCAAGCAGTCAACTTGGAGGGAAGCCCTTTTATGTTCTCCCAGAAGAACATGTTCACTGCAGTAGGTTGCGGAGTTATGGCCACAATCACCTCAAACTCAAATGGCGTTACCATTTCAGCCGGGTGCAGGTCCGAATGTTATGATTATACTGTTAGTAAAAATAGTAGCAGCCATAATACCTGCAATGGTGTGGATTGCTGCCAGACTGTCATCCCTCGTTCCCTCTCTGCCTTCAATACTAGTTTCCAGCGGGCTAATGATAATTACACAAATAGTTCATGTAATTATGCATTTTTGGTTGATCGTGACTGGTTTGAGTCATTTGCCAAAAATTCAACGAATATTTCTGCCACCAGTGATATGGACCAAGTCCCCGTGGAGCTAAAATGGAACTTGTACCATTCAACAACTGATGTTTTTGGGACCTTTATAGAAACAAATGCATCGATGTGGTATGCTGATGACCTAGATGGTCATTGCGAGACTTACAACGACAGCTCTTCATCATATGAATCCTTAAGGCTTGAATGTTCCTGTGGAGGGGGCTCTAAGGGAAATCCATATCTTCTTCAAGGATGCAGAGATATCAATGAATGTGAGGATCCGGAAATCAGAGCTTGTTCTGCAGGGTCCGTATGTGTCAATCAGCCTGGTGGATGGGGATGCAACCCCCCAGAAACCAGCAAGTCGAGGGTTAAAGTAATCTTTATAGGTATCGGCAGTGGTTTTGGACTGATAGTTCTACTAATTGGTGCATGGTGGTTGTACAAACTTTGGAAGAAAAGGAAAAACATTAAACTCAAGAAGAAGTTCTTTAAACAAAATGGGGGACTATTACTAGAGCAGCAACTATCTTCCGGTGAAGTTAATGTTGAGAAAATTAAGCTGTTCAATTCAAAGGAATTAGAAAAAGCCACAGACCGGTTTAATGTAGATAGAATACTCGGCCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGGCCGATGGAAGAATTGTTGCTGTAAAGAAGTCTAAAATAGTTGATGGGGCGAAGAATGTGGTTAAACTATTGGGTTGTTGTTTAGAGACCGAAGTTCCACTTTTGGTTTACGAATTCATACCCAAAGGGACTATCTACCAGTATCTCCATGATCAGAATGAAGAGTTTCCACTCACATGGGAAATGCGCTTAAGGATTTCTGCAGAAGTTGCCGGAGCTCTTTCATACTTACACTCTGCAGCTGCTTTCCCAATTTACCACCGTGATATCAAGTCTACAAACATACTATTAGATGATAAATACAGAGCAAAAGTTGCAGACTTTGGGACTTCAAGATCAGTTTCCATAGACCAAACACACCTAACCACATTTGTACATGGTACATTTGGTTACTTGGATCCGGAGTACTTCCAATCAAGTCAATTTACAGACAAAAGTGACGTGTATAGCTTTGGAGTGGTCCTTGTCGAGCTCTTGACTGGAGAAAAACCAGTTTCTCTGACTAGGTCACAAGAAGCCAGAGGCCTTGTTTCATATTTCAATCTCTCACTGGAGTCGAATAATTTGTTTGATATTATTGATGCTCGAGTTAAGGTGGAGGGTGTAACAGGAGATATTTTGACAGTAGCTAATCTTGCAAAAAGATGTCTGGATATGAATGGGAAGAGACGACCTACCATGAAAGAAGTTGCAATGGAGTTGGAGGTGATTCATAAATCAGTGAAATCGTCAGATATACAACAAAATCATGAAGAGGTTGAGTATGTCCGAAATGAAGTAACTTGTCTTTGGGATGTTGCTTCTACATCAACCAGGTCAGCTTTGGATGGTGGCACTGCTTCATCAATTGATTCACTACCACTAATGTCCAACTGA

Protein Analysis

735

Amino Acids

81.12

Weight (kDa)

5.48

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 4.9e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 461 - 674 4.2e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 461 - 678 6.1e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 167, 465, 536
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 2 cut(s) 210, 1607
AccIII TCCGGA 2 cut(s) 952, 1732
AciI CCGC 2 cut(s) 417, 598
AclWI GGATC 4 cut(s) 944, 957, 1724, 1737
AcoI YGGCCR 3 cut(s) 426, 1294, 1332
AcsI RAATTY 2 cut(s) 679, 1445
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 9 cut(s) 55, 63, 270, 746, 1115, 1312, 1707, 1714, 1739
AfiI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
AflII CTTAAG 2 cut(s) 865, 1522
AflIII ACRYGT 2 cut(s) 396, 1773
AgeI ACCGGT 1 cut(s) 1269
AhlI ACTAGT 2 cut(s) 64, 587
AjiI CACGTC 1 cut(s) 1774
AjnI CCWGG 2 cut(s) 997, 2144
AloI GAACNNNNNNTCC 2 cut(s) 1071, 1103
Alw21I GWGCWC 2 cut(s) 1552, 1806
Alw26I GTCTC 3 cut(s) 824, 1412, 1993
AlwI GGATC 4 cut(s) 944, 957, 1724, 1737
Ama87I CYCGRG 1 cut(s) 1917
Aor13HI TCCGGA 2 cut(s) 952, 1732
AoxI GGCC 5 cut(s) 426, 1294, 1303, 1332, 1855
ApeKI GCWGC 5 cut(s) 516, 546, 1192, 1571, 1574
ApoI RAATTY 2 cut(s) 679, 1445
AsiGI ACCGGT 1 cut(s) 1269
Asp700I GAANNNNTTC 3 cut(s) 398, 1157, 1497
AspLEI GCGC 1 cut(s) 1521
AspS9I GGNCC 5 cut(s) 477, 712, 771, 978, 1792
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 4 cut(s) 307, 427, 1220, 2050
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 5 cut(s) 477, 712, 771, 978, 1792
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 2 cut(s) 949, 1729
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 3 cut(s) 896, 1552, 1806
Bbv12I GWGCWC 2 cut(s) 1552, 1806
BbvI GCAGC 5 cut(s) 528, 533, 1204, 1561, 1583
BccI CCATC 6 cut(s) 136, 812, 999, 1331, 1370, 2153
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 2 cut(s) 999, 2146
BclI TGATCA 1 cut(s) 1483
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 3 cut(s) 824, 1412, 1993
BcuI ACTAGT 2 cut(s) 64, 587
BfaI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1836
BfmI CTRYAG 4 cut(s) 405, 972, 1533, 1569
BfrI CTTAAG 2 cut(s) 865, 1522
BfuAI ACCTGC 3 cut(s) 167, 465, 536
BglI GCCNNNNNGGC 1 cut(s) 1302
BisI GCNGC 5 cut(s) 517, 547, 1193, 1572, 1575
BlsI GCNGC 5 cut(s) 518, 548, 1194, 1573, 1576
BmcAI AGTACT 3 cut(s) 55, 63, 1739
Bme1390I CCNGG 3 cut(s) 469, 999, 2146
Bme18I GGWCC 5 cut(s) 477, 712, 771, 978, 1792
BmeT110I CYCGRG 1 cut(s) 1917
BmgBI CACGTC 1 cut(s) 1774
BmgT120I GGNCC 5 cut(s) 477, 712, 771, 978, 1792
BmiI GGNNCC 5 cut(s) 256, 772, 951, 979, 1731
BmrFI CCNGG 3 cut(s) 469, 999, 2146
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 5 cut(s) 83, 800, 914, 1001, 1903
BmuI ACTGGG 1 cut(s) 4
BpmI CTGGAG 3 cut(s) 284, 1833, 1904
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 1 cut(s) 469
Bsa29I ATCGAT 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 1412
BsaJI CCNNGG 3 cut(s) 723, 1297, 1306
BsaWI WCCGGW 4 cut(s) 952, 1205, 1269, 1732
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 888, 1269
Bse118I RCCGGY 1 cut(s) 1269
Bse1I ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1471, 1816, 1823, 1887
Bse3DI GCAATG 3 cut(s) 538, 823, 2031
BseAI TCCGGA 2 cut(s) 952, 1732
BseBI CCWGG 2 cut(s) 999, 2146
BseCI ATCGAT 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 723, 1297, 1306
BseGI GGATG 6 cut(s) 558, 929, 1010, 1016, 2131, 2164
BseLI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
BseMI GCAATG 3 cut(s) 538, 823, 2031
BseNI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1471, 1816, 1823, 1887
BseXI GCAGC 5 cut(s) 528, 533, 1204, 1561, 1583
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1857
BshNI GGYRCC 1 cut(s) 254
BshTI ACCGGT 1 cut(s) 1269
BshVI ATCGAT 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 1552, 1806
BsiHKCI CYCGRG 1 cut(s) 1917
BsiSI CCGG 6 cut(s) 468, 953, 1206, 1270, 1545, 1733
BslFI GGGAC 5 cut(s) 704, 784, 1191, 1474, 1672
BslI CCNNNNNNNGG 3 cut(s) 474, 888, 1269
BsmAI GTCTC 3 cut(s) 824, 1412, 1993
BsmBI CGTCTC 1 cut(s) 1993
BsmFI GGGAC 5 cut(s) 704, 784, 1191, 1474, 1672
BsnI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1857
Bso31I GGTCTC 1 cut(s) 1412
BsoBI CYCGRG 1 cut(s) 1917
Bsp1286I GDGCHC 3 cut(s) 896, 1552, 1806
Bsp13I TCCGGA 2 cut(s) 952, 1732
Bsp1407I TGTACA 2 cut(s) 1113, 1705
Bsp143I GATC 5 cut(s) 649, 949, 1483, 1667, 1729
BspACI CCGC 2 cut(s) 417, 598
BspANI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1857
BspDI ATCGAT 1 cut(s) 794
BspEI TCCGGA 2 cut(s) 952, 1732
BspHI TCATGA 2 cut(s) 190, 2080
BspLI GGNNCC 5 cut(s) 256, 772, 951, 979, 1731
BspMAI CTGCAG 4 cut(s) 409, 976, 1537, 1573
BspMI ACCTGC 3 cut(s) 167, 465, 536
BspPI GGATC 4 cut(s) 944, 957, 1724, 1737
BspQI GCTCTTC 1 cut(s) 853
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 2 cut(s) 865, 1522
BspTNI GGTCTC 1 cut(s) 1412
BsrDI GCAATG 3 cut(s) 538, 823, 2031
BsrFI RCCGGY 1 cut(s) 1269
BsrGI TGTACA 2 cut(s) 1113, 1705
BsrI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1471, 1816, 1823, 1887
BssAI RCCGGY 1 cut(s) 1269
BssECI CCNNGG 3 cut(s) 723, 1297, 1306
BssMI GATC 5 cut(s) 649, 949, 1483, 1667, 1729
BssNAI GTATAC 1 cut(s) 211
BssT1I CCWWGG 2 cut(s) 1297, 1306
Bst1107I GTATAC 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 999, 2146
Bst4CI ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1595, 1957
Bst6I CTCTTC 4 cut(s) 853, 1488, 1991, 2079
BstAFI CTTAAG 2 cut(s) 865, 1522
BstAPI GCANNNNNTGC 2 cut(s) 1541, 1646
BstAUI TGTACA 2 cut(s) 1113, 1705
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 1 cut(s) 897
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 6 cut(s) 558, 929, 1010, 1016, 2131, 2164
BstHHI GCGC 1 cut(s) 1521
BstKTI GATC 5 cut(s) 652, 952, 1486, 1670, 1732
BstMAI GTCTC 3 cut(s) 824, 1412, 1993
BstMBI GATC 5 cut(s) 649, 949, 1483, 1667, 1729
BstMWI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1541, 1646, 1967
BstNI CCWGG 2 cut(s) 999, 2146
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 467, 997, 2144
BstSFI CTRYAG 4 cut(s) 405, 972, 1533, 1569
BstV1I GCAGC 5 cut(s) 528, 533, 1204, 1561, 1583
BstX2I RGATCY 2 cut(s) 949, 1729
BstXI CCANNNNNNTGG 2 cut(s) 709, 1509
BstYI RGATCY 2 cut(s) 949, 1729
BstZ17I GTATAC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 794
BsuRI GGCC 5 cut(s) 428, 1296, 1305, 1334, 1857
BsuTUI ATCGAT 1 cut(s) 794
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1774
BtsCI GGATG 6 cut(s) 558, 929, 1010, 1016, 2131, 2164
BtsI GCAGTG 4 cut(s) 337, 402, 1075, 2166
BtsIMutI CAGTG 8 cut(s) 225, 337, 402, 709, 1075, 1880, 2058, 2166
BveI ACCTGC 3 cut(s) 167, 465, 536
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 2 cut(s) 190, 2080
CfoI GCGC 1 cut(s) 1521
Cfr10I RCCGGY 1 cut(s) 1269
Cfr13I GGNCC 5 cut(s) 477, 712, 771, 978, 1792
ClaI ATCGAT 1 cut(s) 794
CsiI ACCWGGT 1 cut(s) 2144
Csp6I GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1706, 1713, 1738
CspAI ACCGGT 1 cut(s) 1269
CviAII CATG 9 cut(s) 191, 397, 627, 1104, 1481, 1509, 1709, 2011, 2081
CviQI GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1706, 1713, 1738
DdeI CTNAG 1 cut(s) 897
DpnI GATC 5 cut(s) 651, 951, 1485, 1669, 1731
DpnII GATC 5 cut(s) 649, 949, 1483, 1667, 1729
DraI TTTAAA 1 cut(s) 1165
EaeI YGGCCR 3 cut(s) 426, 1294, 1332
Eam1104I CTCTTC 4 cut(s) 853, 1488, 1991, 2079
EarI CTCTTC 4 cut(s) 853, 1488, 1991, 2079
Ecl136II GAGCTC 2 cut(s) 1550, 1804
Eco130I CCWWGG 2 cut(s) 1297, 1306
Eco147I AGGCCT 1 cut(s) 1857
Eco24I GRGCYC 3 cut(s) 896, 1552, 1806
Eco31I GGTCTC 1 cut(s) 1412
Eco32I GATATC 2 cut(s) 934, 1600
Eco47I GGWCC 5 cut(s) 477, 712, 771, 978, 1792
Eco53kI GAGCTC 2 cut(s) 1550, 1804
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1917
EcoICRI GAGCTC 2 cut(s) 1550, 1804
EcoO109I RGGNCCY 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 1445
EcoRII CCWGG 2 cut(s) 997, 2144
EcoRV GATATC 2 cut(s) 934, 1600
EcoT14I CCWWGG 2 cut(s) 1297, 1306
EcoT22I ATGCAT 2 cut(s) 640, 793
EcoT38I GRGCYC 3 cut(s) 896, 1552, 1806
ErhI CCWWGG 2 cut(s) 1297, 1306
Esp3I CGTCTC 1 cut(s) 1993
FaeI CATG 9 cut(s) 194, 400, 630, 1107, 1484, 1512, 1712, 2014, 2084
FalI AAGNNNNNCTT 4 cut(s) 1145, 1177, 1416, 1448
FaqI GGGAC 5 cut(s) 704, 784, 1191, 1474, 1672
FatI CATG 9 cut(s) 190, 396, 626, 1103, 1480, 1508, 1708, 2010, 2080
FauI CCCGC 1 cut(s) 591
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 1483
FblI GTMKAC 2 cut(s) 210, 1607
Fnu4HI GCNGC 5 cut(s) 517, 547, 1193, 1572, 1575
FokI GGATG 6 cut(s) 545, 936, 1017, 1023, 2138, 2171
FriOI GRGCYC 3 cut(s) 896, 1552, 1806
Fsp4HI GCNGC 5 cut(s) 517, 547, 1193, 1572, 1575
FspBI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1836
GlaI GCGC 1 cut(s) 1520
GluI GCNGC 5 cut(s) 517, 547, 1193, 1572, 1575
GsuI CTGGAG 3 cut(s) 284, 1833, 1904
HaeIII GGCC 5 cut(s) 428, 1296, 1305, 1334, 1857
HapII CCGG 6 cut(s) 468, 953, 1206, 1270, 1545, 1733
HhaI GCGC 1 cut(s) 1521
Hin1II CATG 9 cut(s) 194, 400, 630, 1107, 1484, 1512, 1712, 2014, 2084
Hin6I GCGC 1 cut(s) 1519
HinP1I GCGC 1 cut(s) 1519
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 5 cut(s) 665, 860, 1886, 2041, 2183
HpaII CCGG 6 cut(s) 468, 953, 1206, 1270, 1545, 1733
HphI GGTGA 4 cut(s) 307, 427, 1220, 2050
Hpy166II GTNNAC 6 cut(s) 211, 358, 402, 1318, 1441, 1608
Hpy188I TCNGA 8 cut(s) 79, 305, 481, 962, 1488, 1833, 2065, 2102
Hpy8I GTNNAC 6 cut(s) 211, 358, 402, 1318, 1441, 1608
HpyAV CCTTC 3 cut(s) 281, 328, 589
HpyCH4III ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1595, 1957
HpyCH4IV ACGT 1 cut(s) 1773
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1541, 1646, 1967
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 1 cut(s) 1773
Hsp92II CATG 9 cut(s) 194, 400, 630, 1107, 1484, 1512, 1712, 2014, 2084
HspAI GCGC 1 cut(s) 1519
Kpn2I TCCGGA 2 cut(s) 952, 1732
Ksp22I TGATCA 1 cut(s) 1483
Kzo9I GATC 5 cut(s) 649, 949, 1483, 1667, 1729
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 3 cut(s) 173, 727, 1547
Lsp1109I GCAGC 5 cut(s) 528, 533, 1204, 1561, 1583
LweI GCATC 5 cut(s) 83, 800, 914, 1001, 1903
MabI ACCWGGT 1 cut(s) 2144
MaeI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1836
MaeII ACGT 1 cut(s) 1773
MalI GATC 5 cut(s) 651, 951, 1485, 1669, 1731
MboI GATC 5 cut(s) 649, 949, 1483, 1667, 1729
MfeI CAATTG 1 cut(s) 2178
MflI RGATCY 2 cut(s) 949, 1729
MhlI GDGCHC 3 cut(s) 896, 1552, 1806
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 674, 1895
MmeI TCCRAC 1 cut(s) 2013
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 2 cut(s) 640, 793
MroI TCCGGA 2 cut(s) 952, 1732
MroXI GAANNNNTTC 3 cut(s) 398, 1157, 1497
MscI TGGCCA 1 cut(s) 428
MslI CAYNNNNRTG 2 cut(s) 796, 1507
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 2 cut(s) 598, 1574
MspCI CTTAAG 2 cut(s) 865, 1522
MspI CCGG 6 cut(s) 468, 953, 1206, 1270, 1545, 1733
MspR9I CCNGG 3 cut(s) 469, 999, 2146
MunI CAATTG 1 cut(s) 2178
MvaI CCWGG 2 cut(s) 999, 2146
MwoI GCNNNNNNNGC 8 cut(s) 80, 101, 110, 971, 1302, 1541, 1646, 1967
NciI CCSGG 1 cut(s) 469
NdeI CATATG 1 cut(s) 856
NdeII GATC 5 cut(s) 649, 949, 1483, 1667, 1729
NlaIII CATG 9 cut(s) 194, 400, 630, 1107, 1484, 1512, 1712, 2014, 2084
NlaIV GGNNCC 5 cut(s) 256, 772, 951, 979, 1731
NmeAIII GCCGAG 1 cut(s) 1272
NmuCI GTSAC 4 cut(s) 215, 653, 1769, 1839
NsiI ATGCAT 2 cut(s) 640, 793
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 1917
PagI TCATGA 2 cut(s) 190, 2080
PceI AGGCCT 1 cut(s) 1857
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 853
PdmI GAANNNNTTC 3 cut(s) 398, 1157, 1497
PfeI GAWTC 3 cut(s) 860, 2041, 2183
PflFI GACNNNGTC 2 cut(s) 716, 1837
PinAI ACCGGT 1 cut(s) 1269
PkrI GCNGC 5 cut(s) 518, 548, 1194, 1573, 1576
PleI GAGTC 2 cut(s) 673, 1894
PpsI GAGTC 2 cut(s) 673, 1894
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 396
Psp124BI GAGCTC 2 cut(s) 1552, 1806
Psp5II RGGWCCY 1 cut(s) 771
Psp6I CCWGG 2 cut(s) 997, 2144
PspGI CCWGG 2 cut(s) 997, 2144
PspN4I GGNNCC 5 cut(s) 256, 772, 951, 979, 1731
PspPI GGNCC 5 cut(s) 477, 712, 771, 978, 1792
PspPPI RGGWCCY 1 cut(s) 771
PspXI VCTCGAGB 1 cut(s) 1917
PstI CTGCAG 4 cut(s) 409, 976, 1537, 1573
PsuI RGATCY 2 cut(s) 949, 1729
PsyI GACNNNGTC 2 cut(s) 716, 1837
PvuII CAGCTG 1 cut(s) 1574
RsaI GTAC 9 cut(s) 55, 63, 270, 746, 1115, 1312, 1707, 1714, 1739
RsaNI GTAC 9 cut(s) 54, 62, 269, 745, 1114, 1311, 1706, 1713, 1738
RseI CAYNNNNRTG 2 cut(s) 796, 1507
SacI GAGCTC 2 cut(s) 1552, 1806
SapI GCTCTTC 1 cut(s) 853
SatI GCNGC 5 cut(s) 517, 547, 1193, 1572, 1575
Sau3AI GATC 5 cut(s) 649, 949, 1483, 1667, 1729
Sau96I GGNCC 5 cut(s) 477, 712, 771, 978, 1792
ScaI AGTACT 3 cut(s) 55, 63, 1739
SchI GAGTC 2 cut(s) 674, 1895
ScrFI CCNGG 3 cut(s) 469, 999, 2146
SduI GDGCHC 3 cut(s) 896, 1552, 1806
SexAI ACCWGGT 1 cut(s) 2144
SfaNI GCATC 5 cut(s) 83, 800, 914, 1001, 1903
SfcI CTRYAG 4 cut(s) 405, 972, 1533, 1569
SfiI GGCCNNNNNGGCC 1 cut(s) 1302
Sfr274I CTCGAG 1 cut(s) 1917
SinI GGWCC 5 cut(s) 477, 712, 771, 978, 1792
SlaI CTCGAG 1 cut(s) 1917
SmiMI CAYNNNNRTG 2 cut(s) 796, 1507
SmlI CTYRAG 4 cut(s) 865, 1148, 1522, 1917
SmoI CTYRAG 4 cut(s) 865, 1148, 1522, 1917
SpeI ACTAGT 2 cut(s) 64, 587
SseBI AGGCCT 1 cut(s) 1857
SsiI CCGC 2 cut(s) 417, 598
SspI AATATT 1 cut(s) 691
SspMI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1836
SstI GAGCTC 2 cut(s) 1552, 1806
StuI AGGCCT 1 cut(s) 1857
StyD4I CCNGG 3 cut(s) 467, 997, 2144
StyI CCWWGG 2 cut(s) 1297, 1306
TaaI ACNGT 7 cut(s) 39, 215, 499, 556, 1315, 1595, 1957
TaiI ACGT 1 cut(s) 1776
TaqI TCGA 5 cut(s) 794, 1037, 1800, 1889, 1918
TaqII GACCGA 1 cut(s) 1436
TatI WGTACW 5 cut(s) 53, 61, 1113, 1705, 1737
TfiI GAWTC 3 cut(s) 860, 2041, 2183
TscAI CASTG 8 cut(s) 232, 337, 409, 709, 1075, 1887, 2058, 2173
TseFI GTSAC 4 cut(s) 215, 653, 1769, 1839
TseI GCWGC 5 cut(s) 516, 546, 1192, 1571, 1574
Tsp45I GTSAC 4 cut(s) 215, 653, 1769, 1839
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 8 cut(s) 232, 337, 409, 709, 1075, 1887, 2058, 2173
Tth111I GACNNNGTC 2 cut(s) 716, 1837
Vha464I CTTAAG 2 cut(s) 865, 1522
VpaK11BI GGWCC 5 cut(s) 477, 712, 771, 978, 1792
XapI RAATTY 2 cut(s) 679, 1445
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 706, 2152
XhoI CTCGAG 1 cut(s) 1917
XmiI GTMKAC 2 cut(s) 210, 1607
XmnI GAANNNNTTC 3 cut(s) 398, 1157, 1497
XspI CTAG 6 cut(s) 65, 272, 588, 815, 1187, 1836
ZrmI AGTACT 3 cut(s) 55, 63, 1739
Zsp2I ATGCAT 2 cut(s) 640, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.