Rw2G040830

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000052
Physical Location & Seq
Reverse (-)
66476224 .. 66478842
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040830.1

Sequence Viewer

Length: 2190 bp
ATGGTACCTGTTCTGTTTTTACTTCATGTGATCACTCTGTTCTTGTGGTCTATGAGTACTATTGCGGCGCAGCCGGTAGCATCAGAAGCCGTACCTATAGCAAAGCCTAATTGTACACAAGATTGCGGAAACGTTAAAATCCCATATCCTTTTGGAATTGAAGCAGGTTGTTACATAGACGACTGGTTCAAAATTTTCTGTTACAAACCCACCGGCAAGTTCGCCAGCCAACCCACAGCTTACTTGAACCCCGCCAATCTGAACCTTGTGGTCTTCAATATTTCTCTTGAAGCAGGAACAATACAGGTAACAACTAACTTCACCTTTTCTAGTCTTGGCTGCGTTGATCAGCGAACTGCCGTCCTACCAGCCAACTTGAGCGGAAGCCCGTTCCTCTACTCCGATAAAAACAAATTCACTGTAATTGGATGCGGTGGAATCACCTGGATGATGCTCTCCAACGGTTCAAAAGTTGGTGGCTGCCTGGCCGCTTGTGACGGCAGTGATCCTGCACAACGTGAATTCCTTGATGGCTGCACAGGTATCAACTCATGCTGCCAGACCAACATTCCTCCGAATCTTACTGCTTTCAACTACAGCTATCAAGCAATAGATACAACTTACCCTGGAAATGACTCTTGCAAGAAGGCATTTTTGGTAAACCCGGATTGGGTTGGGTACTCAAGCGGAGGAATGGTATCCATAAAGCAACTTGCCATCCCTATGTTGCTCGACTGGAGATTAAGTAATTACTCAACTCTTCAAATACGTGGAACAGCTGATTGGGCCAATAACTCGGGCACGACATGCACAAATGCTAGTTTGTGTTCCTGCTCATCCGGGTTTCAGGGAAACCCCTACCTTCCCAACGGATGTCAAGATATCAATGAATGTGAAGATTCAGACCCATGTCCTGGTGCCAGCATTTGTATCAACGTTCGTGGTTCGTTTAGGTGCCAATCTCCGGATCAAACGGTTAAACTGGCAATTATTGTTGTCGGCAGTGTTCTTGGACTATTGCTTATACTCATTTGTGCATGGGGGTCACACAATGTCATAAAGAAAAGAAAAAACACTAAACGCAAGGAGAAGTTTTTTAAGCAGAATGGTGGTTTGCTGCTGGAGAAACAATCTTCTGGTGACGTTAATGTTGAGAAAATTAAATTGTTCAATTCTGAGGAGTTGGAGAAAGCCACAGATAATTATAATGCAAACAGAATTCTAGGTCAAGGAGGCCATGGAACTGTTTACAAAGGCATGCTCGCAGATGGAAGTATTGTGGCGATAAAGAAGTCTAAAATAGTTGATGAAGGAGAAGTTGAACAATTCATTAATGAAATTGTTATTCTTTCACAAATAATTCATAGGAATGTGGTTAAATTAATGGGTTGTTGTTTAGAGACCGAAGTTCCCCTTCTAGTTTATGAATTCATACCAAATGGAACTCTTTCTCAGTATATTCATCACCAGAATGAGGAGTTTCCTCTTACATGGAGAGTGCGGTTGCGAGTTTCTATTGAAGTGGCAGGAGCTCTTTCCTACTTACACTCAGCAACTTCCTTTCCCATTTACCATCGAGATATCAAGACCTCCAACATACTCTTGGATGATAAATTTAGAGCTAAAGTTGCAGACTTTGGAACTTCCAGAACAGTTGCCATCGACAAAACACATCTTACCATGTCACAAGTTCAAGGAACATTTGGCTATTTAGACCCAGAGTACTTCCAGTCTAGCCAATTTACAGATAAGAGTGATGTTTATAGCTTTGGAGTGGTCCTTGTTGAACTTCTAACTGGGCAAAAGCCAGTTTCTCAGACGAGGCCAGAAGAGTGGAGAAGCCTGACAAATTATTTCCTTCTTTCAATGGAGGAGAATCGTCTGCTTGACATTCTTGATGCTCGAGTTATGAATGAAGGTGGCAAGGAGGAAATTATGACAGTTGCACAGCTTGCAAGGCGTTGCTTGAATATGAAGGGAAAGAAGCGTCCTACTATGAAAGAAGTTGCAGTTCAATTGGAGGGAATATTTCAATTATCAGCAAAAGATTCTGACGTTCATACAAATTCAGCAGAGGTTGAATATGTCCTAAATGGATGGCATGTTGGTTCTACTTCAGCACGATATAACACAATCCATATTGGTAGTGGTTCTCCATCGGATGTAGAACCACTTATATATGTTTCTTGA

Protein Analysis

729

Amino Acids

80.22

Weight (kDa)

6.11

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 104 2.3e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 294 - 320 1e-05 Calcium-binding EGF domain
Pkinase PF00069 402 - 669 4.8e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 405 - 673 6.3e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1206
Acc36I ACCTGC 1 cut(s) 155
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 3 cut(s) 4, 917, 954
AccB7I CCANNNNNTGG 1 cut(s) 914
AccBSI CCGCTC 1 cut(s) 381
AccIII TCCGGA 1 cut(s) 964
AciI CCGC 8 cut(s) 65, 126, 252, 381, 432, 489, 687, 1501
AclI AACGTT 2 cut(s) 132, 936
AclWI GGATC 2 cut(s) 500, 975
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 7 cut(s) 192, 413, 521, 1218, 1427, 1613, 2065
AcuI CTGAAG 1 cut(s) 2100
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 6 cut(s) 6, 58, 93, 115, 680, 1724
AfiI CCNNNNNNNGG 4 cut(s) 670, 914, 964, 1474
AjnI CCWGG 4 cut(s) 443, 483, 625, 913
AjuI GAANNNNNNNTTGG 4 cut(s) 638, 670, 766, 798
AluBI AGCT 7 cut(s) 239, 600, 779, 1532, 1622, 1767, 1951
AluI AGCT 7 cut(s) 239, 600, 779, 1532, 1622, 1767, 1951
Alw21I GWGCWC 1 cut(s) 1534
Alw26I GTCTC 1 cut(s) 1394
AlwI GGATC 2 cut(s) 500, 975
Ama87I CYCGRG 2 cut(s) 796, 1902
Aor13HI TCCGGA 1 cut(s) 964
AoxI GGCC 4 cut(s) 486, 786, 1234, 1823
ApeKI GCWGC 6 cut(s) 70, 339, 480, 534, 555, 1117
ApoI RAATTY 7 cut(s) 192, 413, 521, 1218, 1427, 1613, 2065
AseI ATTAAT 2 cut(s) 1332, 1382
Asp700I GAANNNNTTC 1 cut(s) 1447
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 786, 1777
AsuC2I CCSGG 2 cut(s) 665, 841
AsuHPI GGTGA 4 cut(s) 313, 433, 1151, 1457
AvaI CYCGRG 2 cut(s) 796, 1902
AvaII GGWCC 1 cut(s) 1777
BaeGI GKGCMC 1 cut(s) 803
BanI GGYRCC 3 cut(s) 4, 917, 954
BanII GRGCYC 1 cut(s) 1534
BbsI GAAGAC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 1534
BbvI GCAGC 6 cut(s) 82, 326, 467, 521, 542, 1104
BccI CCATC 7 cut(s) 524, 725, 1262, 1581, 1667, 2091, 2164
BceAI ACGGC 3 cut(s) 74, 344, 514
BciT130I CCWGG 4 cut(s) 445, 485, 627, 915
BciVI GTATCC 1 cut(s) 709
BclI TGATCA 2 cut(s) 30, 346
BcnI CCSGG 2 cut(s) 665, 841
BcoDI GTCTC 1 cut(s) 1394
BfaI CTAG 5 cut(s) 330, 819, 1223, 1418, 1734
BfmI CTRYAG 2 cut(s) 96, 595
BfuAI ACCTGC 1 cut(s) 155
BfuI GTATCC 1 cut(s) 709
BisI GCNGC 8 cut(s) 66, 71, 340, 481, 489, 535, 556, 1118
BlsI GCNGC 8 cut(s) 67, 72, 341, 482, 490, 536, 557, 1119
BmcAI AGTACT 2 cut(s) 58, 1724
Bme1390I CCNGG 6 cut(s) 445, 485, 627, 665, 841, 915
Bme18I GGWCC 1 cut(s) 1777
BmeT110I CYCGRG 2 cut(s) 796, 1902
BmgT120I GGNCC 2 cut(s) 786, 1777
BmiI GGNNCC 3 cut(s) 6, 919, 956
BmrFI CCNGG 6 cut(s) 445, 485, 627, 665, 841, 915
BmrI ACTGGG 1 cut(s) 1806
BmsI GCATC 4 cut(s) 89, 419, 441, 1888
BmuI ACTGGG 1 cut(s) 1806
BoxI GACNNNNGTC 1 cut(s) 909
BpiI GAAGAC 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 757, 1142
BpuEI CTTGAG 2 cut(s) 397, 667
BpuMI CCSGG 2 cut(s) 665, 841
BsaAI YACGTR 1 cut(s) 770
BsaI GGTCTC 1 cut(s) 1394
BsaJI CCNNGG 2 cut(s) 625, 1237
BsaWI WCCGGW 1 cut(s) 964
BsaXI ACNNNNNCTCC 4 cut(s) 681, 711, 730, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 670, 914, 964, 1474
Bse118I RCCGGY 2 cut(s) 73, 212
Bse1I ACTGG 6 cut(s) 188, 740, 987, 1729, 1801, 1808
BseAI TCCGGA 1 cut(s) 964
BseBI CCWGG 4 cut(s) 445, 485, 627, 915
BseDI CCNNGG 2 cut(s) 625, 1237
BseGI GGATG 8 cut(s) 434, 453, 717, 836, 878, 1612, 2102, 2167
BseLI CCNNNNNNNGG 4 cut(s) 670, 914, 964, 1474
BseMII CTCAG 4 cut(s) 1167, 1466, 1563, 1829
BseNI ACTGG 6 cut(s) 188, 740, 987, 1729, 1801, 1808
BseRI GAGGAG 3 cut(s) 1193, 1490, 1886
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 6 cut(s) 82, 326, 467, 521, 542, 1104
BsgI GTGCAG 2 cut(s) 495, 520
BshFI GGCC 4 cut(s) 488, 788, 1236, 1825
BshNI GGYRCC 3 cut(s) 4, 917, 954
BsiHKAI GWGCWC 1 cut(s) 1534
BsiHKCI CYCGRG 2 cut(s) 796, 1902
BsiSI CCGG 5 cut(s) 74, 213, 665, 840, 965
BslI CCNNNNNNNGG 4 cut(s) 670, 914, 964, 1474
BsmAI GTCTC 1 cut(s) 1394
BsnI GGCC 4 cut(s) 488, 788, 1236, 1825
Bso31I GGTCTC 1 cut(s) 1394
BsoBI CYCGRG 2 cut(s) 796, 1902
Bsp1286I GDGCHC 2 cut(s) 803, 1534
Bsp13I TCCGGA 1 cut(s) 964
Bsp1407I TGTACA 1 cut(s) 113
Bsp143I GATC 4 cut(s) 30, 346, 505, 967
Bsp19I CCATGG 1 cut(s) 1237
BspACI CCGC 8 cut(s) 65, 126, 252, 381, 432, 489, 687, 1501
BspANI GGCC 4 cut(s) 488, 788, 1236, 1825
BspCNI CTCAG 4 cut(s) 1168, 1465, 1562, 1828
BspEI TCCGGA 1 cut(s) 964
BspLI GGNNCC 3 cut(s) 6, 919, 956
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 2 cut(s) 500, 975
BspT107I GGYRCC 3 cut(s) 4, 917, 954
BspTNI GGTCTC 1 cut(s) 1394
BsrBI CCGCTC 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 73, 212
BsrGI TGTACA 1 cut(s) 113
BsrI ACTGG 6 cut(s) 188, 740, 987, 1729, 1801, 1808
BssAI RCCGGY 2 cut(s) 73, 212
BssECI CCNNGG 2 cut(s) 625, 1237
BssMI GATC 4 cut(s) 30, 346, 505, 967
BssT1I CCWWGG 1 cut(s) 1237
Bst2UI CCWGG 4 cut(s) 445, 485, 627, 915
Bst4CI ACNGT 6 cut(s) 421, 464, 976, 1246, 1654, 1942
Bst6I CTCTTC 2 cut(s) 765, 1824
BstAPI GCANNNNNTGC 2 cut(s) 807, 1952
BstAUI TGTACA 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 770
BstC8I GCNNGC 5 cut(s) 226, 922, 1259, 1263, 1953
BstDEI CTNAG 4 cut(s) 1176, 1452, 1549, 1815
BstDSI CCRYGG 1 cut(s) 1237
BstF5I GGATG 8 cut(s) 434, 453, 717, 836, 878, 1612, 2102, 2167
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 4 cut(s) 33, 349, 508, 970
BstMAI GTCTC 1 cut(s) 1394
BstMBI GATC 4 cut(s) 30, 346, 505, 967
BstMWI GCNNNNNNNGC 6 cut(s) 86, 785, 807, 1628, 1952, 1957
BstNI CCWGG 4 cut(s) 445, 485, 627, 915
BstNSI RCATGY 3 cut(s) 810, 1261, 2105
BstPAI GACNNNNGTC 1 cut(s) 909
BstSCI CCNGG 6 cut(s) 443, 483, 625, 663, 839, 913
BstSFI CTRYAG 2 cut(s) 96, 595
BstSLI GKGCMC 1 cut(s) 803
BstV1I GCAGC 6 cut(s) 82, 326, 467, 521, 542, 1104
BstV2I GAAGAC 1 cut(s) 265
BstXI CCANNNNNNTGG 1 cut(s) 1833
BsuI GTATCC 1 cut(s) 709
BsuRI GGCC 4 cut(s) 488, 788, 1236, 1825
BtgI CCRYGG 1 cut(s) 1237
BtsCI GGATG 8 cut(s) 434, 453, 717, 836, 878, 1612, 2102, 2167
BtsI GCAGTG 2 cut(s) 508, 1009
BtsIMutI CAGTG 3 cut(s) 417, 508, 1009
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 5 cut(s) 226, 922, 1259, 1263, 1953
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 2 cut(s) 73, 212
Cfr13I GGNCC 2 cut(s) 786, 1777
CseI GACGC 1 cut(s) 1976
Csp6I GTAC 6 cut(s) 5, 57, 92, 114, 679, 1723
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviQI GTAC 6 cut(s) 5, 57, 92, 114, 679, 1723
DdeI CTNAG 4 cut(s) 1176, 1452, 1549, 1815
DpnI GATC 4 cut(s) 32, 348, 507, 969
DpnII GATC 4 cut(s) 30, 346, 505, 967
DraIII CACNNNGTG 1 cut(s) 518
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 2 cut(s) 765, 1824
EarI CTCTTC 2 cut(s) 765, 1824
Ecl136II GAGCTC 1 cut(s) 1532
Eco130I CCWWGG 1 cut(s) 1237
Eco24I GRGCYC 1 cut(s) 1534
Eco31I GGTCTC 1 cut(s) 1394
Eco32I GATATC 2 cut(s) 883, 1582
Eco47I GGWCC 1 cut(s) 1777
Eco53kI GAGCTC 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 2100
Eco88I CYCGRG 2 cut(s) 796, 1902
EcoICRI GAGCTC 1 cut(s) 1532
EcoRI GAATTC 3 cut(s) 521, 1218, 1427
EcoRII CCWGG 4 cut(s) 443, 483, 625, 913
EcoRV GATATC 2 cut(s) 883, 1582
EcoT14I CCWWGG 1 cut(s) 1237
EcoT38I GRGCYC 1 cut(s) 1534
ErhI CCWWGG 1 cut(s) 1237
FalI AAGNNNNNCTT 2 cut(s) 1398, 1430
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 2 cut(s) 30, 346
Fnu4HI GCNGC 8 cut(s) 66, 71, 340, 481, 489, 535, 556, 1118
FokI GGATG 8 cut(s) 441, 460, 704, 823, 885, 1619, 2109, 2174
FriOI GRGCYC 1 cut(s) 1534
Fsp4HI GCNGC 8 cut(s) 66, 71, 340, 481, 489, 535, 556, 1118
FspBI CTAG 5 cut(s) 330, 819, 1223, 1418, 1734
GlaI GCGC 1 cut(s) 69
GluI GCNGC 8 cut(s) 66, 71, 340, 481, 489, 535, 556, 1118
GsuI CTGGAG 2 cut(s) 757, 1142
HaeIII GGCC 4 cut(s) 488, 788, 1236, 1825
HapII CCGG 5 cut(s) 74, 213, 665, 840, 965
HgaI GACGC 1 cut(s) 1976
HhaI GCGC 1 cut(s) 70
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 6 cut(s) 438, 577, 635, 899, 1876, 2048
HpaII CCGG 5 cut(s) 74, 213, 665, 840, 965
HphI GGTGA 4 cut(s) 313, 433, 1151, 1457
Hpy166II GTNNAC 3 cut(s) 116, 661, 1249
Hpy188I TCNGA 9 cut(s) 85, 261, 403, 576, 904, 1177, 1818, 2053, 2161
Hpy188III TCNNGA 8 cut(s) 287, 878, 965, 1577, 1585, 1647, 1895, 2187
Hpy8I GTNNAC 3 cut(s) 116, 661, 1249
HpyAV CCTTC 7 cut(s) 640, 872, 1304, 1424, 1868, 1910, 1969
HpyCH4III ACNGT 6 cut(s) 421, 464, 976, 1246, 1654, 1942
HpyCH4IV ACGT 6 cut(s) 132, 517, 769, 936, 1143, 2055
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 785, 807, 1628, 1952, 1957
HpyF3I CTNAG 4 cut(s) 1176, 1452, 1549, 1815
HpySE526I ACGT 6 cut(s) 132, 517, 769, 936, 1143, 2055
HspAI GCGC 1 cut(s) 68
Kpn2I TCCGGA 1 cut(s) 964
KpnI GGTACC 1 cut(s) 8
Ksp22I TGATCA 2 cut(s) 30, 346
Kzo9I GATC 4 cut(s) 30, 346, 505, 967
LmnI GCTCC 1 cut(s) 1529
Lsp1109I GCAGC 6 cut(s) 82, 326, 467, 521, 542, 1104
LweI GCATC 4 cut(s) 89, 419, 441, 1888
MaeI CTAG 5 cut(s) 330, 819, 1223, 1418, 1734
MaeII ACGT 6 cut(s) 132, 517, 769, 936, 1143, 2055
MaeIII GTNAC 7 cut(s) 170, 200, 307, 494, 1044, 1139, 1683
MalI GATC 4 cut(s) 32, 348, 507, 969
MbiI CCGCTC 1 cut(s) 381
MboI GATC 4 cut(s) 30, 346, 505, 967
MboII GAAGA 5 cut(s) 265, 752, 908, 1125, 1841
MfeI CAATTG 1 cut(s) 2015
MhlI GDGCHC 2 cut(s) 803, 1534
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 3 cut(s) 483, 1164, 1617
MroI TCCGGA 1 cut(s) 964
MroXI GAANNNNTTC 1 cut(s) 1447
MseI TTAA 9 cut(s) 135, 743, 978, 1098, 1146, 1161, 1332, 1377, 1382
MslI CAYNNNNRTG 4 cut(s) 446, 722, 1368, 1470
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 5 cut(s) 74, 213, 665, 840, 965
MspR9I CCNGG 6 cut(s) 445, 485, 627, 665, 841, 915
MunI CAATTG 1 cut(s) 2015
MvaI CCWGG 4 cut(s) 445, 485, 627, 915
MwoI GCNNNNNNNGC 6 cut(s) 86, 785, 807, 1628, 1952, 1957
NciI CCSGG 2 cut(s) 665, 841
NcoI CCATGG 1 cut(s) 1237
NdeII GATC 4 cut(s) 30, 346, 505, 967
NlaIV GGNNCC 3 cut(s) 6, 919, 956
NmuCI GTSAC 4 cut(s) 494, 1044, 1139, 1683
NspI RCATGY 3 cut(s) 810, 1261, 2105
PaeI GCATGC 1 cut(s) 1261
PaeR7I CTCGAG 1 cut(s) 1902
PdmI GAANNNNTTC 1 cut(s) 1447
PfeI GAWTC 5 cut(s) 438, 577, 899, 1876, 2048
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 8 cut(s) 67, 72, 341, 482, 490, 536, 557, 1119
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 909
PshBI ATTAAT 2 cut(s) 1332, 1382
PsiI TTATAA 1 cut(s) 1206
Psp124BI GAGCTC 1 cut(s) 1534
Psp1406I AACGTT 2 cut(s) 132, 936
Psp6I CCWGG 4 cut(s) 443, 483, 625, 913
PspGI CCWGG 4 cut(s) 443, 483, 625, 913
PspN4I GGNNCC 3 cut(s) 6, 919, 956
PspPI GGNCC 2 cut(s) 786, 1777
PspXI VCTCGAGB 1 cut(s) 1902
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 6 cut(s) 6, 58, 93, 115, 680, 1724
RsaNI GTAC 6 cut(s) 5, 57, 92, 114, 679, 1723
RseI CAYNNNNRTG 4 cut(s) 446, 722, 1368, 1470
SacI GAGCTC 1 cut(s) 1534
SaqAI TTAA 9 cut(s) 135, 743, 978, 1098, 1146, 1161, 1332, 1377, 1382
SatI GCNGC 8 cut(s) 66, 71, 340, 481, 489, 535, 556, 1118
Sau3AI GATC 4 cut(s) 30, 346, 505, 967
Sau96I GGNCC 2 cut(s) 786, 1777
ScaI AGTACT 2 cut(s) 58, 1724
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 6 cut(s) 445, 485, 627, 665, 841, 915
SduI GDGCHC 2 cut(s) 803, 1534
SfaNI GCATC 4 cut(s) 89, 419, 441, 1888
SfcI CTRYAG 2 cut(s) 96, 595
Sfr274I CTCGAG 1 cut(s) 1902
SinI GGWCC 1 cut(s) 1777
SlaI CTCGAG 1 cut(s) 1902
SmiMI CAYNNNNRTG 4 cut(s) 446, 722, 1368, 1470
SmlI CTYRAG 3 cut(s) 376, 682, 1902
SmoI CTYRAG 3 cut(s) 376, 682, 1902
SphI GCATGC 1 cut(s) 1261
SsiI CCGC 8 cut(s) 65, 126, 252, 381, 432, 489, 687, 1501
SspI AATATT 2 cut(s) 280, 2028
SspMI CTAG 5 cut(s) 330, 819, 1223, 1418, 1734
SstI GAGCTC 1 cut(s) 1534
StyD4I CCNGG 6 cut(s) 443, 483, 625, 663, 839, 913
StyI CCWWGG 1 cut(s) 1237
TaaI ACNGT 6 cut(s) 421, 464, 976, 1246, 1654, 1942
TaiI ACGT 6 cut(s) 135, 520, 772, 939, 1146, 2058
TaqI TCGA 4 cut(s) 732, 1576, 1662, 1903
TaqII GACCGA 1 cut(s) 1418
TatI WGTACW 3 cut(s) 56, 113, 1722
TauI GCSGC 2 cut(s) 68, 491
TfiI GAWTC 5 cut(s) 438, 577, 899, 1876, 2048
Tru1I TTAA 9 cut(s) 135, 743, 978, 1098, 1146, 1161, 1332, 1377, 1382
Tru9I TTAA 9 cut(s) 135, 743, 978, 1098, 1146, 1161, 1332, 1377, 1382
TscAI CASTG 3 cut(s) 424, 508, 1009
TseFI GTSAC 4 cut(s) 494, 1044, 1139, 1683
TseI GCWGC 6 cut(s) 70, 339, 480, 534, 555, 1117
Tsp45I GTSAC 4 cut(s) 494, 1044, 1139, 1683
TspGWI ACGGA 1 cut(s) 885
TspRI CASTG 3 cut(s) 424, 508, 1009
Van91I CCANNNNNTGG 1 cut(s) 914
VpaK11BI GGWCC 1 cut(s) 1777
VspI ATTAAT 2 cut(s) 1332, 1382
XapI RAATTY 7 cut(s) 192, 413, 521, 1218, 1427, 1613, 2065
XceI RCATGY 3 cut(s) 810, 1261, 2105
XcmI CCANNNNNNNNNTGG 2 cut(s) 1600, 2144
XhoI CTCGAG 1 cut(s) 1902
XmnI GAANNNNTTC 1 cut(s) 1447
XspI CTAG 5 cut(s) 330, 819, 1223, 1418, 1734
ZrmI AGTACT 2 cut(s) 58, 1724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.