Rw5G010520

Wall-associated receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000052
Physical Location & Seq
Forward (+)
11555008 .. 11560973
5966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010520.1

Sequence Viewer

Length: 2259 bp
ATGGAGTTGCGACTCTCTTTGGTGGCAGTCCTACTGCTGTTATCGGCGGCTAGTACTACTACGTCGACAACAGCAGCTCCTGCTCAAGCCAAGCTTGGCTGCTCAGACAAGTGCGGCAATCTCACAATTCCATATCCATTTGGCATGAAGAAGGATTGTTACCTGCGAGAAGAATTCTTTATCAATTGTAGCCATACCACCCAATCACCAGTGGCATATTTGATGGAAGGGAGTCTCATTGTTACTAACATTTCCCTTGATGACGGTGAGTTGGGAATATTGGGCTCCGTAGCTGCAGATTGCTATAACGCCCAGGGCAATTTGACGTGCAGCTTCCCTTCTTCGATCAGCCTGCCTCTTCCTTACACGATCTCCAGTTCCAAAAGCAAGTTCTATGCCGTTGGTTGTGACGTTCGTGCAATTATACAAGGCTTCCGAGGAGGAGAAGAGTTGATGTCCGGGTGCATATCCGTATGCAACAGCCTTGACAACGTCGATAAGAACTCTTGCTCCGGCGCTGGGTGTTGCCAGACTAACATCCCCTGCGGACTAAAGAATACGTCGGTCAAGGTGGATAGGTACAAAAATAATTTGAGAGTAAGGGATTTTAACCCCTGCAGCTACGCCTTTATTGTAGAACAAGGCCAGTTTAATTTCTCCCCTGCGAGTTTTAAAGAGCTGAAAAACACTGAAAAGCTTCCAATGGTTCTTAATTGGGCAATTGGGAAGGACACAAATCCCTGTAATGAAGCTCAAAAGAGGAAGGATTTCATATGCACGGCTAATAGCAGGTGCGTCAACCCTAACAGGGGGTCAGTAGGTTACTTGTGCCGGTGCTTGCCTGGTTATGAAGGAAACCCTTACCACCCAGGTGGTTGCCAAGATATTGATGAGTGCAGGGCTTCAAACCCCTGCCAGAACGGACGGTGCTTAAATACTCCACCTCCAGGAAAATACTCTTGTTCATGTCACAAAGGATACAAAACCGACGGCATGAATGACAAGCTTTGCATAAAAGACGATTCCAAACGACACTCAAAGATCGTTCTCCTGCAGATTCCATTGTACGTTGCATGGTGGTTGGTCCAAGTCATAAAGAAAAGGAAAAACAAAAAACGAAAGGAGAAGTTCTTGAAACAAAATGGCGGTTTAGTATTGGAGCAACAATTATCTTCTGGTGATCAAGCTAATGTTGAAAAAATTAAGTTGTTCAATTCAAGGGAGTTGGAGAAAGCCACGGACCATTTTAGCATAGATAGAATTCTTGGCCAGGGAGGCCAAGGAAATGTTTACAAAGGAATGTTGATGGATCGACAAATTGTTGCGGTCAAAAAGTCTAAAATAGTTAACGGAGGCGAAGTTCGACAATTCATTAATGAAATTGTGATTCTTTCACAAATTAACCACAGAAATGTGGTTAAACTATTGGGTTGTTGTTTGGAGACTGAAATTCCCCTCTTGGTCTATGAATTTCTACCGAACGGAACACTTTCTCAGTACATTCATGACCAAGATGAGGAGTTTCCTCTCACATGGGGAACGCGATTGCGAGTGTCAACCGAAGTTGCTGGAGCTCTTTCATATTTACATTCAGCAGCTTCTATTCCTATTTACCACCGGGACATCAAGTCCACGAACATACTCTTGGATGCCAAGTATAGAGCAAAAATTGCAGATTTTGGGATTTCAAGATCCATTTCCACTGACCAGACACATCTCACCACACAAGTACATGGAACATTTGGTTATTTGGACCCAGAGTACTTTCAATCGGGCCAATTTACAAAAAAGAGTGATGTTTACAGCTTCGGTGTGGTCCTTGCTGAGCTCTTGACCGGACAAAAACCAGTTATTTCCATGATGAGGTCGCAAGAATGTAGAAGCCTAGCAACTCATTTTCTTCTTTCCATGGAGCAAAATCGTTTGTTTTATATTCTTGACGCTCAAGTTAAGAAAGATGGTAGCAAAGAAGAAATTATAGCAGTTGCTAACCTTGCGCAAAGATGCTTAAATTTGAGTGGAAGGAAACGTCCTACTATGAAAGAAGTAGCGGCGGAATTGGAGGGAGTTCGATTGGCAGTTAAAGATGACGATCATGTTCAACAAAATTTGGTAGAGGAAGGAGATGTCGAAACTCATGAAATAACCAGTGAGGCTTATTGCGATGTTGTTTCTACATCACACGGTCCTTTTACAGATGGTGGTACTGCAAGTTCTTCATCTGATGTACAACCACTAATGTTTTACAATACACAGTAG

Protein Analysis

752

Amino Acids

83.19

Weight (kDa)

8.08

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 91 2.5e-10 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 417 - 684 1.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 418 - 687 5.8e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 780
Acc16I TGCGCA 1 cut(s) 1998
Acc36I ACCTGC 2 cut(s) 171, 780
AccI GTMKAC 1 cut(s) 65
AccII CGCG 1 cut(s) 1543
AciI CCGC 7 cut(s) 47, 114, 546, 1146, 1325, 2051, 2054
AclWI GGATC 2 cut(s) 1317, 1686
AcoI YGGCCR 1 cut(s) 1267
AcsI RAATTY 6 cut(s) 173, 1260, 1449, 1469, 2011, 2107
AfaI GTAC 8 cut(s) 55, 581, 1067, 1499, 1731, 1763, 2206, 2229
AfiI CCNNNNNNNGG 6 cut(s) 519, 808, 809, 947, 1516, 1863
AgsI TTSAA 8 cut(s) 906, 1135, 1196, 1213, 1218, 1689, 1769, 2102
AhdI GACNNNNNGTC 1 cut(s) 1627
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 5 cut(s) 312, 841, 868, 946, 1269
AjuI GAANNNNNNNTTGG 4 cut(s) 374, 406, 1628, 1660
AleI CACNNNNGTG 1 cut(s) 870
AloI GAACNNNNNNTCC 4 cut(s) 494, 526, 1344, 1376
Alw21I GWGCWC 2 cut(s) 1576, 1830
Alw26I GTCTC 2 cut(s) 239, 1436
AlwI GGATC 2 cut(s) 1317, 1686
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 4 cut(s) 643, 1267, 1276, 1774
ApeKI GCWGC 6 cut(s) 74, 99, 293, 330, 618, 1595
ApoI RAATTY 6 cut(s) 173, 1260, 1449, 1469, 2011, 2107
AseI ATTAAT 1 cut(s) 1374
Asp700I GAANNNNTTC 3 cut(s) 696, 767, 1489
AspLEI GCGC 2 cut(s) 518, 1999
AspS9I GGNCC 6 cut(s) 1084, 1240, 1753, 1774, 1816, 2186
AsuC2I CCSGG 2 cut(s) 460, 1619
AsuHPI GGTGA 4 cut(s) 198, 278, 1190, 1711
AvaII GGWCC 5 cut(s) 1084, 1240, 1753, 1816, 2186
BalI TGGCCA 1 cut(s) 1269
BanII GRGCYC 3 cut(s) 287, 1576, 1830
BarI GAAGNNNNNNTAC 2 cut(s) 143, 175
Bbv12I GWGCWC 2 cut(s) 1576, 1830
BbvI GCAGC 6 cut(s) 86, 86, 280, 342, 630, 1607
BccI CCATC 4 cut(s) 217, 1300, 1952, 2192
BceAI ACGGC 3 cut(s) 383, 795, 1006
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 5 cut(s) 314, 843, 870, 948, 1271
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 1180
BcnI CCSGG 2 cut(s) 460, 1619
BcoDI GTCTC 2 cut(s) 239, 1436
BfaI CTAG 2 cut(s) 51, 1886
BfmI CTRYAG 3 cut(s) 294, 616, 1052
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 2 cut(s) 171, 780
BfuI GTATCC 1 cut(s) 971
BglI GCCNNNNNGGC 1 cut(s) 1275
BisI GCNGC 9 cut(s) 48, 75, 100, 115, 294, 331, 619, 1596, 2052
BlpI GCTNAGC 1 cut(s) 1824
BlsI GCNGC 9 cut(s) 49, 76, 101, 116, 295, 332, 620, 1597, 2053
BmcAI AGTACT 2 cut(s) 55, 1763
Bme1390I CCNGG 7 cut(s) 314, 460, 843, 870, 948, 1271, 1619
Bme18I GGWCC 5 cut(s) 1084, 1240, 1753, 1816, 2186
BmeRI GACNNNNNGTC 1 cut(s) 1627
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 6 cut(s) 1084, 1240, 1753, 1774, 1816, 2186
BmiI GGNNCC 2 cut(s) 286, 1755
BmrFI CCNGG 7 cut(s) 314, 460, 843, 870, 948, 1271, 1619
BmsI GCATC 2 cut(s) 1639, 1994
BpmI CTGGAG 3 cut(s) 358, 930, 1590
Bpu1102I GCTNAGC 1 cut(s) 1824
BpuEI CTTGAG 2 cut(s) 69, 1929
BpuMI CCSGG 2 cut(s) 460, 1619
BsaBI GATNNNNATC 1 cut(s) 2091
BsaJI CCNNGG 8 cut(s) 312, 313, 436, 868, 1236, 1270, 1279, 1908
BsaWI WCCGGW 1 cut(s) 1835
Bsc4I CCNNNNNNNGG 6 cut(s) 519, 808, 809, 947, 1516, 1863
Bse118I RCCGGY 1 cut(s) 831
Bse1I ACTGG 5 cut(s) 209, 375, 646, 1847, 2148
Bse8I GATNNNNATC 1 cut(s) 2091
BseBI CCWGG 5 cut(s) 314, 843, 870, 948, 1271
BseDI CCNNGG 8 cut(s) 312, 313, 436, 868, 1236, 1270, 1279, 1908
BseGI GGATG 2 cut(s) 537, 1654
BseJI GATNNNNATC 1 cut(s) 2091
BseLI CCNNNNNNNGG 6 cut(s) 519, 808, 809, 947, 1516, 1863
BseMII CTCAG 3 cut(s) 117, 1508, 1815
BseNI ACTGG 5 cut(s) 209, 375, 646, 1847, 2148
BseRI GAGGAG 3 cut(s) 453, 456, 1532
BseXI GCAGC 6 cut(s) 86, 86, 280, 342, 630, 1607
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 2 cut(s) 349, 916
Bsh1236I CGCG 1 cut(s) 1543
BshFI GGCC 4 cut(s) 645, 1269, 1278, 1776
BsiHKAI GWGCWC 2 cut(s) 1576, 1830
BsiSI CCGG 5 cut(s) 459, 513, 832, 1618, 1836
BslFI GGGAC 1 cut(s) 1634
BslI CCNNNNNNNGG 6 cut(s) 519, 808, 809, 947, 1516, 1863
BsmAI GTCTC 2 cut(s) 239, 1436
BsmFI GGGAC 1 cut(s) 1634
BsnI GGCC 4 cut(s) 645, 1269, 1278, 1776
Bsp1286I GDGCHC 3 cut(s) 287, 1576, 1830
Bsp1407I TGTACA 1 cut(s) 2227
Bsp143I GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
Bsp1720I GCTNAGC 1 cut(s) 1824
Bsp19I CCATGG 1 cut(s) 1908
BspACI CCGC 7 cut(s) 47, 114, 546, 1146, 1325, 2051, 2054
BspANI GGCC 4 cut(s) 645, 1269, 1278, 1776
BspCNI CTCAG 3 cut(s) 116, 1507, 1816
BspFNI CGCG 1 cut(s) 1543
BspHI TCATGA 2 cut(s) 1504, 2137
BspLI GGNNCC 2 cut(s) 286, 1755
BspMAI CTGCAG 3 cut(s) 298, 620, 1056
BspMI ACCTGC 2 cut(s) 171, 780
BspPI GGATC 2 cut(s) 1317, 1686
BsrFI RCCGGY 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 2227
BsrI ACTGG 5 cut(s) 209, 375, 646, 1847, 2148
BssAI RCCGGY 1 cut(s) 831
BssECI CCNNGG 8 cut(s) 312, 313, 436, 868, 1236, 1270, 1279, 1908
BssMI GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
BssT1I CCWWGG 2 cut(s) 1279, 1908
Bst2UI CCWGG 5 cut(s) 314, 843, 870, 948, 1271
Bst4CI ACNGT 4 cut(s) 266, 927, 2186, 2256
Bst6I CTCTTC 2 cut(s) 363, 441
BstAPI GCANNNNNTGC 2 cut(s) 80, 1670
BstAUI TGTACA 1 cut(s) 2227
BstC8I GCNNGC 2 cut(s) 353, 839
BstDEI CTNAG 3 cut(s) 103, 1494, 1824
BstDSI CCRYGG 2 cut(s) 1236, 1908
BstF5I GGATG 2 cut(s) 537, 1654
BstFNI CGCG 1 cut(s) 1543
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 2 cut(s) 518, 1999
BstKTI GATC 7 cut(s) 348, 372, 1044, 1183, 1312, 1694, 2095
BstMAI GTCTC 2 cut(s) 239, 1436
BstMBI GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
BstMWI GCNNNNNNNGC 4 cut(s) 80, 1275, 1670, 1994
BstNI CCWGG 5 cut(s) 314, 843, 870, 948, 1271
BstSCI CCNGG 7 cut(s) 312, 458, 841, 868, 946, 1269, 1617
BstSFI CTRYAG 3 cut(s) 294, 616, 1052
BstUI CGCG 1 cut(s) 1543
BstV1I GCAGC 6 cut(s) 86, 86, 280, 342, 630, 1607
BstX2I RGATCY 1 cut(s) 1691
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 1 cut(s) 1691
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 4 cut(s) 645, 1269, 1278, 1776
BtgI CCRYGG 2 cut(s) 1236, 1908
BtgZI GCGATG 1 cut(s) 2178
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 2 cut(s) 537, 1654
BtsIMutI CAGTG 4 cut(s) 216, 687, 1701, 2155
BveI ACCTGC 2 cut(s) 171, 780
Cac8I GCNNGC 2 cut(s) 353, 839
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 2 cut(s) 1504, 2137
CfoI GCGC 2 cut(s) 518, 1999
Cfr10I RCCGGY 1 cut(s) 831
Cfr13I GGNCC 6 cut(s) 1084, 1240, 1753, 1774, 1816, 2186
CseI GACGC 2 cut(s) 784, 1949
Csp6I GTAC 8 cut(s) 54, 580, 1066, 1498, 1730, 1762, 2205, 2228
CviQI GTAC 8 cut(s) 54, 580, 1066, 1498, 1730, 1762, 2205, 2228
DdeI CTNAG 3 cut(s) 103, 1494, 1824
DpnI GATC 7 cut(s) 347, 371, 1043, 1182, 1311, 1693, 2094
DpnII GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
DraI TTTAAA 1 cut(s) 673
DriI GACNNNNNGTC 1 cut(s) 1627
EaeI YGGCCR 1 cut(s) 1267
Eam1104I CTCTTC 2 cut(s) 363, 441
Eam1105I GACNNNNNGTC 1 cut(s) 1627
EarI CTCTTC 2 cut(s) 363, 441
EciI GGCGGA 1 cut(s) 2069
Ecl136II GAGCTC 2 cut(s) 1574, 1828
Eco130I CCWWGG 2 cut(s) 1279, 1908
Eco24I GRGCYC 3 cut(s) 287, 1576, 1830
Eco47I GGWCC 5 cut(s) 1084, 1240, 1753, 1816, 2186
Eco53kI GAGCTC 2 cut(s) 1574, 1828
EcoICRI GAGCTC 2 cut(s) 1574, 1828
EcoRI GAATTC 2 cut(s) 173, 1260
EcoRII CCWGG 5 cut(s) 312, 841, 868, 946, 1269
EcoT14I CCWWGG 2 cut(s) 1279, 1908
EcoT38I GRGCYC 3 cut(s) 287, 1576, 1830
ErhI CCWWGG 2 cut(s) 1279, 1908
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FaqI GGGAC 1 cut(s) 1634
FauNDI CATATG 1 cut(s) 773
FbaI TGATCA 1 cut(s) 1180
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 9 cut(s) 48, 75, 100, 115, 294, 331, 619, 1596, 2052
FokI GGATG 2 cut(s) 524, 1661
FriOI GRGCYC 3 cut(s) 287, 1576, 1830
Fsp4HI GCNGC 9 cut(s) 48, 75, 100, 115, 294, 331, 619, 1596, 2052
FspBI CTAG 2 cut(s) 51, 1886
FspI TGCGCA 1 cut(s) 1998
GlaI GCGC 2 cut(s) 517, 1998
GluI GCNGC 9 cut(s) 48, 75, 100, 115, 294, 331, 619, 1596, 2052
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 3 cut(s) 358, 930, 1590
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 4 cut(s) 645, 1269, 1278, 1776
HapII CCGG 5 cut(s) 459, 513, 832, 1618, 1836
HgaI GACGC 2 cut(s) 784, 1949
HhaI GCGC 2 cut(s) 518, 1999
Hin6I GCGC 2 cut(s) 516, 1997
HinP1I GCGC 2 cut(s) 516, 1997
HincII GTYRAC 4 cut(s) 66, 799, 1348, 1557
HindII GTYRAC 4 cut(s) 66, 799, 1348, 1557
HindIII AAGCTT 3 cut(s) 92, 695, 1004
HinfI GANTC 5 cut(s) 12, 232, 1022, 1057, 1387
HpaI GTTAAC 1 cut(s) 1348
HpaII CCGG 5 cut(s) 459, 513, 832, 1618, 1836
HphI GGTGA 4 cut(s) 198, 278, 1190, 1711
Hpy166II GTNNAC 7 cut(s) 66, 799, 1291, 1348, 1557, 1632, 1801
Hpy188I TCNGA 3 cut(s) 106, 437, 2224
Hpy188III TCNNGA 6 cut(s) 1132, 1505, 1689, 1831, 1937, 2138
Hpy8I GTNNAC 7 cut(s) 66, 799, 1291, 1348, 1557, 1632, 1801
Hpy99I CGWCG 4 cut(s) 67, 497, 565, 992
HpyAV CCTTC 8 cut(s) 145, 221, 348, 721, 757, 845, 2016, 2114
HpyCH4III ACNGT 4 cut(s) 266, 927, 2186, 2256
HpyCH4IV ACGT 7 cut(s) 62, 326, 411, 492, 560, 1068, 2029
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 1275, 1670, 1994
HpyF3I CTNAG 3 cut(s) 103, 1494, 1824
HpySE526I ACGT 7 cut(s) 62, 326, 411, 492, 560, 1068, 2029
HspAI GCGC 2 cut(s) 516, 1997
Ksp22I TGATCA 1 cut(s) 1180
KspAI GTTAAC 1 cut(s) 1348
Kzo9I GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
LmnI GCTCC 6 cut(s) 82, 290, 515, 1159, 1571, 1912
Lsp1109I GCAGC 6 cut(s) 86, 86, 280, 342, 630, 1607
LweI GCATC 2 cut(s) 1639, 1994
MaeI CTAG 2 cut(s) 51, 1886
MaeII ACGT 7 cut(s) 62, 326, 411, 492, 560, 1068, 2029
MaeIII GTNAC 5 cut(s) 158, 241, 407, 821, 968
MalI GATC 7 cut(s) 347, 371, 1043, 1182, 1311, 1693, 2094
MboI GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
MboII GAAGA 9 cut(s) 160, 182, 333, 350, 458, 1164, 1892, 1982, 2208
MfeI CAATTG 2 cut(s) 184, 720
MflI RGATCY 1 cut(s) 1691
MhlI GDGCHC 3 cut(s) 287, 1576, 1830
MlsI TGGCCA 1 cut(s) 1269
MluNI TGGCCA 1 cut(s) 1269
MlyI GAGTC 2 cut(s) 6, 241
MmeI TCCRAC 1 cut(s) 1206
Mox20I TGGCCA 1 cut(s) 1269
MroXI GAANNNNTTC 3 cut(s) 696, 767, 1489
MscI TGGCCA 1 cut(s) 1269
MslI CAYNNNNRTG 2 cut(s) 870, 1410
Msp20I TGGCCA 1 cut(s) 1269
MspI CCGG 5 cut(s) 459, 513, 832, 1618, 1836
MspR9I CCNGG 7 cut(s) 314, 460, 843, 870, 948, 1271, 1619
MunI CAATTG 2 cut(s) 184, 720
MvaI CCWGG 5 cut(s) 314, 843, 870, 948, 1271
MvnI CGCG 1 cut(s) 1543
MwoI GCNNNNNNNGC 4 cut(s) 80, 1275, 1670, 1994
NciI CCSGG 2 cut(s) 460, 1619
NcoI CCATGG 1 cut(s) 1908
NdeI CATATG 1 cut(s) 773
NdeII GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
NlaIV GGNNCC 2 cut(s) 286, 1755
NmuCI GTSAC 2 cut(s) 407, 968
NsbI TGCGCA 1 cut(s) 1998
OliI CACNNNNGTG 1 cut(s) 870
PagI TCATGA 2 cut(s) 1504, 2137
PaqCI CACCTGC 1 cut(s) 780
PasI CCCWGGG 1 cut(s) 313
PdmI GAANNNNTTC 3 cut(s) 696, 767, 1489
PfeI GAWTC 3 cut(s) 1022, 1057, 1387
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 946
PkrI GCNGC 9 cut(s) 49, 76, 101, 116, 295, 332, 620, 1597, 2053
PleI GAGTC 2 cut(s) 6, 240
PpsI GAGTC 2 cut(s) 6, 240
PshBI ATTAAT 1 cut(s) 1374
Psp124BI GAGCTC 2 cut(s) 1576, 1830
Psp6I CCWGG 5 cut(s) 312, 841, 868, 946, 1269
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 5 cut(s) 312, 841, 868, 946, 1269
PspN4I GGNNCC 2 cut(s) 286, 1755
PspPI GGNCC 6 cut(s) 1084, 1240, 1753, 1774, 1816, 2186
PsrI GAACNNNNNNTAC 2 cut(s) 2197, 2229
PstI CTGCAG 3 cut(s) 298, 620, 1056
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 1691
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 8 cut(s) 55, 581, 1067, 1499, 1731, 1763, 2206, 2229
RsaNI GTAC 8 cut(s) 54, 580, 1066, 1498, 1730, 1762, 2205, 2228
RseI CAYNNNNRTG 2 cut(s) 870, 1410
SacI GAGCTC 2 cut(s) 1576, 1830
SalI GTCGAC 1 cut(s) 64
SatI GCNGC 9 cut(s) 48, 75, 100, 115, 294, 331, 619, 1596, 2052
Sau3AI GATC 7 cut(s) 345, 369, 1041, 1180, 1309, 1691, 2092
Sau96I GGNCC 6 cut(s) 1084, 1240, 1753, 1774, 1816, 2186
ScaI AGTACT 2 cut(s) 55, 1763
SchI GAGTC 2 cut(s) 6, 241
ScrFI CCNGG 7 cut(s) 314, 460, 843, 870, 948, 1271, 1619
SduI GDGCHC 3 cut(s) 287, 1576, 1830
SfaNI GCATC 2 cut(s) 1639, 1994
SfcI CTRYAG 3 cut(s) 294, 616, 1052
SfiI GGCCNNNNNGGCC 1 cut(s) 1275
SinI GGWCC 5 cut(s) 1084, 1240, 1753, 1816, 2186
SmiMI CAYNNNNRTG 2 cut(s) 870, 1410
SmlI CTYRAG 2 cut(s) 84, 1944
SmoI CTYRAG 2 cut(s) 84, 1944
SsiI CCGC 7 cut(s) 47, 114, 546, 1146, 1325, 2051, 2054
SspI AATATT 1 cut(s) 279
SspMI CTAG 2 cut(s) 51, 1886
SstI GAGCTC 2 cut(s) 1576, 1830
StyD4I CCNGG 7 cut(s) 312, 458, 841, 868, 946, 1269, 1617
StyI CCWWGG 2 cut(s) 1279, 1908
TaaI ACNGT 4 cut(s) 266, 927, 2186, 2256
TaiI ACGT 7 cut(s) 65, 329, 414, 495, 563, 1071, 2032
TaqI TCGA 7 cut(s) 65, 344, 495, 1312, 1363, 2071, 2130
TaqII GACCGA 1 cut(s) 553
TatI WGTACW 5 cut(s) 53, 1497, 1729, 1761, 2227
TauI GCSGC 3 cut(s) 50, 117, 2054
TfiI GAWTC 3 cut(s) 1022, 1057, 1387
TscAI CASTG 4 cut(s) 216, 694, 1708, 2155
TseFI GTSAC 2 cut(s) 407, 968
TseI GCWGC 6 cut(s) 74, 99, 293, 330, 618, 1595
Tsp45I GTSAC 2 cut(s) 407, 968
TspGWI ACGGA 6 cut(s) 277, 460, 936, 1253, 1365, 1497
TspRI CASTG 4 cut(s) 216, 694, 1708, 2155
Tth111I GACNNNGTC 1 cut(s) 491
VpaK11BI GGWCC 5 cut(s) 1084, 1240, 1753, 1816, 2186
VspI ATTAAT 1 cut(s) 1374
XapI RAATTY 6 cut(s) 173, 1260, 1449, 1469, 2011, 2107
XmiI GTMKAC 1 cut(s) 65
XmnI GAANNNNTTC 3 cut(s) 696, 767, 1489
XspI CTAG 2 cut(s) 51, 1886
ZrmI AGTACT 2 cut(s) 55, 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.