Rroxscaffold_2G00126500

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000052
Physical Location & Seq
Forward (+)
61830664 .. 61833571
2908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126500.1

Sequence Viewer

Length: 1989 bp
ATGAGTACTGGTACTCCAACACTAGCAGTATCAGAGGCAGTAGTACCCATGACAAAGCCTGGTTGTTCAACGCATTGCGGAGATGTTGAAATTCCATATCCTTTTGGGATTGAACCTGGTTGTTACATTAATGAATGGTTCCAAATACTCTGCGGAAGCTCCACCGGCCTTCTCAAGCCTCTCTTGAACAGCATGCATCTGGAGGTGTGGGATATTTCTGTTGCAGGCACGATAAGGGTCACAAACCCAATCACCTTCTCAAATTGCAGTAGCACCCAGGTCAACGCTGTCCAGACATTCACTCTGGAGGGAAGCCCTTTTCTGTTCTCCCAGAAAAATAGGTTTACTTCAATCAGTTGCAACGAAATTGCTCTGCTGACTTCATCAGATGGATATCCAATTCGTGGTTGCTTATCTATTTGTGATTTCAAATCTTTTGTTAGCAGTACTGGGAGAACACTAAACTACAACAGTTGCAGTGGTACAAACTGCTGCCAGTCCACAATTCCGCTTGATCTTCATGCCTTCAATACTAGTTTTCAGAAGTTGAATGATGTGAAGATTGGAAGTGCCTGCAATCCAGCATTCCTGGTAGACCAGGATTGGTTTATGTCAAACTCAATAAACATTTCTCCGGCTGGTGAAATATTTGACTCCATCCCTGTAGTGCTCGAATGGAGCTTATACAACTATTCGCCTCCCGCCCTTGGTGAGTATGACAGTTATGTCGAATCCTTCTACTGCGAAAGAATTCAAAATAAAATGCAGCCCCAGAACATTAGTACTCGATGTTTCTGCTCGCAGGGCTTTCAAGGAAATCCATATCTTTTTCAGGGATGTCAACAAGATATCGATGAGTGTGGTTCTGGAACTTTCGTAGTCCATCGTGGGAGTCCTATCTGCCAAAGATCCTCTCCGAATTCTCTGGGACAGGCAGCAGGGCTAGGCCTTGGACTACTAATCTTCCTCATAGTGGTCGCACGGTGGTTGAACAGGGTCATAAGGAAAAGGAAACACAATAAACGCAAGCGGATGTTTTTTAAACAAAATGGTGGTTTACTGTTGCAGCAACAATTTTCTAACGGCGAAGTCCATGTTGAGAAAATTAAGTTGTTCACTTCCAAGGAGTTGCTCAAAGCCACAGACGAGTTTAATGTAAACAGAGTTCTTGGCCATGGAAGTCAAGGTACCGTTTACAAAGGAATGTTGGAAGATGGAAAAATTGTTGCTGTAAAGAAGTCCAAAATAGTTGATGGAGGCGAAGTTGGACGGTTCATTAATGAAATTGGCATTCTTTCACAAATCAACCACAGGAATGTCGTCAAGCTATTAGGTTGTTGCTTAGAGACAGAAGTTCCTCTTTTGGTTTACGAATTCATACTCAATGGAAGCCTTTCTCAATACATTCATCATCAAAATGATTTTCGTCTCACATGGGAAATGCGCGTACGAGTTTCTATGCAAGTTGCGGGAGCTCTTTCCTACTTGCATTCATCTACTTGCTTGCCCATTTACCATCGAGACATCAAGTCCGGTAACATACTCCTGGATGATAAATTTAATGCCAAAATAGCTGATTTTGGGACTTCAAGATCCATTAGCATTGACAAAACGCATCTTACAACACTCGTAAAAGGAACATTTGGTTATCTAGATCCAGGATATTTTCAATCCAGCCAATTTACGGACAAGAGTGACGTTTATAGCTTTGGAGTGGTCCTTGTTGAGCTCTTAAGTGGACAAAAACCAGTTTTCTTGACACCATCTGGAGAATGGAGTAGCCTAGCAACTCATTTCATTGAGTCCATGGAAGAAAATAGTCTGTTTGATATTATTGATGCGCGAGTTATGAAGGATGGTGGGAAGGAAGAGATCATGGCAGTTGCTAACCTTGCCAAAAGATGCTTGGATTTGAATGGAAAGAAACGTCCAAATATGAAAGAAGTAGCGGCGACGCTAGAGGAAATTCTACTGTTAGTTACTAGTTAG

Protein Analysis

662

Amino Acids

73.37

Weight (kDa)

6.7

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 22 - 81 1.5e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 384 - 650 4.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 652 1.3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 725
Acc65I GGTACC 1 cut(s) 1187
AccB1I GGYRCC 1 cut(s) 1187
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 594
AccII CGCG 2 cut(s) 1446, 1843
AciI CCGC 7 cut(s) 78, 153, 509, 702, 1030, 1469, 1949
AclWI GGATC 3 cut(s) 903, 1587, 1649
AcoI YGGCCR 1 cut(s) 1171
AcsI RAATTY 6 cut(s) 90, 750, 919, 1373, 1556, 1965
AfaI GTAC 8 cut(s) 7, 13, 45, 448, 484, 784, 1189, 1449
AfiI CCNNNNNNNGG 4 cut(s) 404, 707, 973, 1684
AflII CTTAAG 1 cut(s) 1732
AhdI GACNNNNNGTC 1 cut(s) 1528
AhlI ACTAGT 2 cut(s) 533, 1982
AjnI CCWGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
AluBI AGCT 7 cut(s) 159, 681, 1327, 1475, 1574, 1707, 1729
AluI AGCT 7 cut(s) 159, 681, 1327, 1475, 1574, 1707, 1729
Alw21I GWGCWC 3 cut(s) 672, 1477, 1731
Alw26I GTCTC 3 cut(s) 1340, 1433, 1515
AlwI GGATC 3 cut(s) 903, 1587, 1649
AoxI GGCC 3 cut(s) 166, 946, 1171
ApeKI GCWGC 4 cut(s) 492, 766, 935, 1066
ApoI RAATTY 6 cut(s) 90, 750, 919, 1373, 1556, 1965
AseI ATTAAT 2 cut(s) 129, 1278
Asp700I GAANNNNTTC 2 cut(s) 750, 1393
Asp718I GGTACC 1 cut(s) 1187
AspLEI GCGC 2 cut(s) 1446, 1843
AspS9I GGNCC 1 cut(s) 1717
AsuHPI GGTGA 3 cut(s) 244, 653, 722
AvaII GGWCC 1 cut(s) 1717
BalI TGGCCA 1 cut(s) 1173
BanI GGYRCC 1 cut(s) 1187
BanII GRGCYC 2 cut(s) 1477, 1731
BarI GAAGNNNNNNTAC 2 cut(s) 1171, 1203
Bbv12I GWGCWC 3 cut(s) 672, 1477, 1731
BbvI GCAGC 4 cut(s) 479, 778, 947, 1078
BccI CCATC 8 cut(s) 383, 665, 891, 1208, 1247, 1524, 1771, 1850
BceAI ACGGC 1 cut(s) 1099
BcgI CGANNNNNNTGC 2 cut(s) 777, 811
BciT130I CCWGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
BcoDI GTCTC 3 cut(s) 1340, 1433, 1515
BcuI ACTAGT 2 cut(s) 533, 1982
BfaI CTAG 7 cut(s) 23, 534, 944, 1652, 1784, 1958, 1983
BfmI CTRYAG 1 cut(s) 663
BfrI CTTAAG 1 cut(s) 1732
BisI GCNGC 5 cut(s) 493, 767, 936, 1067, 1950
BlsI GCNGC 5 cut(s) 494, 768, 937, 1068, 1951
BmcAI AGTACT 3 cut(s) 7, 448, 784
Bme1390I CCNGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
Bme18I GGWCC 1 cut(s) 1717
BmeRI GACNNNNNGTC 1 cut(s) 1528
BmgT120I GGNCC 1 cut(s) 1717
BmiI GGNNCC 2 cut(s) 140, 1189
BmrFI CCNGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 4 cut(s) 205, 1624, 1828, 1892
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 3 cut(s) 221, 326, 1788
BpuEI CTTGAG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 852
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 6 cut(s) 276, 706, 949, 1122, 1174, 1806
BsaWI WCCGGW 1 cut(s) 1532
BsaXI ACNNNNNCTCC 3 cut(s) 28, 1248, 1278
Bsc4I CCNNNNNNNGG 4 cut(s) 404, 707, 973, 1684
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 4 cut(s) 13, 454, 496, 1748
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 393
BseBI CCWGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
BseCI ATCGAT 1 cut(s) 852
BseDI CCNNGG 6 cut(s) 276, 706, 949, 1122, 1174, 1806
BseGI GGATG 5 cut(s) 657, 842, 1038, 1555, 1861
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 4 cut(s) 404, 707, 973, 1684
BseMI GCAATG 1 cut(s) 73
BseNI ACTGG 4 cut(s) 13, 454, 496, 1748
BseXI GCAGC 4 cut(s) 479, 778, 947, 1078
Bsh1236I CGCG 2 cut(s) 1446, 1843
BshFI GGCC 3 cut(s) 168, 948, 1173
BshNI GGYRCC 1 cut(s) 1187
BshVI ATCGAT 1 cut(s) 852
BsiHKAI GWGCWC 3 cut(s) 672, 1477, 1731
BsiSI CCGG 3 cut(s) 165, 635, 1533
BsiWI CGTACG 1 cut(s) 1447
BslFI GGGAC 2 cut(s) 942, 1597
BslI CCNNNNNNNGG 4 cut(s) 404, 707, 973, 1684
BsmAI GTCTC 3 cut(s) 1340, 1433, 1515
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 2 cut(s) 942, 1597
BsmI GAATGC 3 cut(s) 584, 1290, 1489
BsnI GGCC 3 cut(s) 168, 948, 1173
Bsp1286I GDGCHC 3 cut(s) 672, 1477, 1731
Bsp143I GATC 5 cut(s) 514, 908, 1592, 1654, 1872
Bsp19I CCATGG 2 cut(s) 1174, 1806
BspACI CCGC 7 cut(s) 78, 153, 509, 702, 1030, 1469, 1949
BspANI GGCC 3 cut(s) 168, 948, 1173
BspDI ATCGAT 1 cut(s) 852
BspFNI CGCG 2 cut(s) 1446, 1843
BspLI GGNNCC 2 cut(s) 140, 1189
BspPI GGATC 3 cut(s) 903, 1587, 1649
BspT107I GGYRCC 1 cut(s) 1187
BspTI CTTAAG 1 cut(s) 1732
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 4 cut(s) 13, 454, 496, 1748
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 6 cut(s) 276, 706, 949, 1122, 1174, 1806
BssMI GATC 5 cut(s) 514, 908, 1592, 1654, 1872
BssT1I CCWWGG 5 cut(s) 706, 949, 1122, 1174, 1806
Bst2UI CCWGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
Bst4CI ACNGT 7 cut(s) 473, 722, 984, 1062, 1192, 1272, 1974
Bst6I CTCTTC 1 cut(s) 1863
BstAFI CTTAAG 1 cut(s) 1732
BstC8I GCNNGC 6 cut(s) 194, 226, 574, 800, 1028, 1505
BstDEI CTNAG 1 cut(s) 1342
BstDSI CCRYGG 2 cut(s) 1174, 1806
BstF5I GGATG 5 cut(s) 657, 842, 1038, 1555, 1861
BstFNI CGCG 2 cut(s) 1446, 1843
BstHHI GCGC 2 cut(s) 1446, 1843
BstKTI GATC 5 cut(s) 517, 911, 1595, 1657, 1875
BstMAI GTCTC 3 cut(s) 1340, 1433, 1515
BstMBI GATC 5 cut(s) 514, 908, 1592, 1654, 1872
BstMWI GCNNNNNNNGC 4 cut(s) 165, 804, 1571, 1892
BstNI CCWGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 2 cut(s) 1446, 1843
BstV1I GCAGC 4 cut(s) 479, 778, 947, 1078
BstX2I RGATCY 3 cut(s) 908, 1592, 1654
BstYI RGATCY 3 cut(s) 908, 1592, 1654
Bsu15I ATCGAT 1 cut(s) 852
BsuRI GGCC 3 cut(s) 168, 948, 1173
BsuTUI ATCGAT 1 cut(s) 852
BtgI CCRYGG 2 cut(s) 1174, 1806
BtsCI GGATG 5 cut(s) 657, 842, 1038, 1555, 1861
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 484
Cac8I GCNNGC 6 cut(s) 194, 226, 574, 800, 1028, 1505
CfoI GCGC 2 cut(s) 1446, 1843
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 1717
ClaI ATCGAT 1 cut(s) 852
CseI GACGC 1 cut(s) 1963
CsiI ACCWGGT 1 cut(s) 115
Csp6I GTAC 8 cut(s) 6, 12, 44, 447, 483, 783, 1188, 1448
CviAII CATG 8 cut(s) 49, 193, 521, 1094, 1175, 1434, 1807, 1876
CviQI GTAC 8 cut(s) 6, 12, 44, 447, 483, 783, 1188, 1448
DdeI CTNAG 1 cut(s) 1342
DpnI GATC 5 cut(s) 516, 910, 1594, 1656, 1874
DpnII GATC 5 cut(s) 514, 908, 1592, 1654, 1872
DraI TTTAAA 1 cut(s) 1042
DrdI GACNNNNNNGTC 1 cut(s) 725
DriI GACNNNNNGTC 1 cut(s) 1528
DseDI GACNNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 1171
Eam1104I CTCTTC 1 cut(s) 1863
Eam1105I GACNNNNNGTC 1 cut(s) 1528
EarI CTCTTC 1 cut(s) 1863
Ecl136II GAGCTC 2 cut(s) 1475, 1729
Eco130I CCWWGG 5 cut(s) 706, 949, 1122, 1174, 1806
Eco147I AGGCCT 1 cut(s) 948
Eco24I GRGCYC 2 cut(s) 1477, 1731
Eco32I GATATC 2 cut(s) 395, 850
Eco47I GGWCC 1 cut(s) 1717
Eco53kI GAGCTC 2 cut(s) 1475, 1729
EcoICRI GAGCTC 2 cut(s) 1475, 1729
EcoRI GAATTC 3 cut(s) 750, 919, 1373
EcoRII CCWGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
EcoRV GATATC 2 cut(s) 395, 850
EcoT14I CCWWGG 5 cut(s) 706, 949, 1122, 1174, 1806
EcoT22I ATGCAT 1 cut(s) 198
EcoT38I GRGCYC 2 cut(s) 1477, 1731
ErhI CCWWGG 5 cut(s) 706, 949, 1122, 1174, 1806
Esp3I CGTCTC 1 cut(s) 1433
FaeI CATG 8 cut(s) 52, 196, 524, 1097, 1178, 1437, 1810, 1879
FalI AAGNNNNNCTT 4 cut(s) 167, 199, 1344, 1376
FaqI GGGAC 2 cut(s) 942, 1597
FatI CATG 8 cut(s) 48, 192, 520, 1093, 1174, 1433, 1806, 1875
FauI CCCGC 2 cut(s) 709, 1462
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 5 cut(s) 493, 767, 936, 1067, 1950
FokI GGATG 5 cut(s) 644, 849, 1045, 1562, 1868
FriOI GRGCYC 2 cut(s) 1477, 1731
Fsp4HI GCNGC 5 cut(s) 493, 767, 936, 1067, 1950
FspBI CTAG 7 cut(s) 23, 534, 944, 1652, 1784, 1958, 1983
GlaI GCGC 2 cut(s) 1445, 1842
GluI GCNGC 5 cut(s) 493, 767, 936, 1067, 1950
GsuI CTGGAG 3 cut(s) 221, 326, 1788
HaeIII GGCC 3 cut(s) 168, 948, 1173
HapII CCGG 3 cut(s) 165, 635, 1533
HgaI GACGC 1 cut(s) 1963
HhaI GCGC 2 cut(s) 1446, 1843
Hin1II CATG 8 cut(s) 52, 196, 524, 1097, 1178, 1437, 1810, 1879
Hin6I GCGC 2 cut(s) 1444, 1841
HinP1I GCGC 2 cut(s) 1444, 1841
HincII GTYRAC 2 cut(s) 283, 842
HindII GTYRAC 2 cut(s) 283, 842
HinfI GANTC 4 cut(s) 653, 731, 892, 1802
HpaII CCGG 3 cut(s) 165, 635, 1533
HphI GGTGA 3 cut(s) 244, 653, 722
Hpy188I TCNGA 4 cut(s) 34, 388, 543, 918
Hpy99I CGWCG 1 cut(s) 1957
HpyAV CCTTC 6 cut(s) 179, 265, 535, 745, 1846, 1858
HpyCH4III ACNGT 7 cut(s) 473, 722, 984, 1062, 1192, 1272, 1974
HpyCH4IV ACGT 2 cut(s) 1698, 1927
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 804, 1571, 1892
HpyF3I CTNAG 1 cut(s) 1342
HpySE526I ACGT 2 cut(s) 1698, 1927
Hsp92II CATG 8 cut(s) 52, 196, 524, 1097, 1178, 1437, 1810, 1879
HspAI GCGC 2 cut(s) 1444, 1841
KpnI GGTACC 1 cut(s) 1191
Kzo9I GATC 5 cut(s) 514, 908, 1592, 1654, 1872
LmnI GCTCC 3 cut(s) 164, 678, 1472
Lsp1109I GCAGC 4 cut(s) 479, 778, 947, 1078
LweI GCATC 4 cut(s) 205, 1624, 1828, 1892
MabI ACCWGGT 1 cut(s) 115
MaeI CTAG 7 cut(s) 23, 534, 944, 1652, 1784, 1958, 1983
MaeII ACGT 2 cut(s) 1698, 1927
MaeIII GTNAC 5 cut(s) 122, 238, 1535, 1694, 1978
MalI GATC 5 cut(s) 516, 910, 1594, 1656, 1874
MboI GATC 5 cut(s) 514, 908, 1592, 1654, 1872
MboII GAAGA 6 cut(s) 509, 571, 955, 1223, 1823, 1880
MflI RGATCY 3 cut(s) 908, 1592, 1654
MhlI GDGCHC 3 cut(s) 672, 1477, 1731
MlsI TGGCCA 1 cut(s) 1173
MluNI TGGCCA 1 cut(s) 1173
MlyI GAGTC 3 cut(s) 647, 901, 1811
MmeI TCCRAC 3 cut(s) 41, 1188, 1246
Mox20I TGGCCA 1 cut(s) 1173
Mph1103I ATGCAT 1 cut(s) 198
MroXI GAANNNNTTC 2 cut(s) 750, 1393
MscI TGGCCA 1 cut(s) 1173
MseI TTAA 7 cut(s) 129, 1041, 1107, 1152, 1278, 1560, 1733
MslI CAYNNNNRTG 2 cut(s) 1314, 1416
Msp20I TGGCCA 1 cut(s) 1173
MspCI CTTAAG 1 cut(s) 1732
MspI CCGG 3 cut(s) 165, 635, 1533
MspR9I CCNGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
Mva1269I GAATGC 3 cut(s) 584, 1290, 1489
MvaI CCWGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
MvnI CGCG 2 cut(s) 1446, 1843
MwoI GCNNNNNNNGC 4 cut(s) 165, 804, 1571, 1892
NcoI CCATGG 2 cut(s) 1174, 1806
NdeII GATC 5 cut(s) 514, 908, 1592, 1654, 1872
NlaIII CATG 8 cut(s) 52, 196, 524, 1097, 1178, 1437, 1810, 1879
NlaIV GGNNCC 2 cut(s) 140, 1189
NmuCI GTSAC 2 cut(s) 238, 1694
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 1 cut(s) 196
PaeI GCATGC 1 cut(s) 196
PceI AGGCCT 1 cut(s) 948
PctI GAATGC 3 cut(s) 584, 1290, 1489
PdmI GAANNNNTTC 2 cut(s) 750, 1393
PfeI GAWTC 1 cut(s) 731
Pfl23II CGTACG 1 cut(s) 1447
PflMI CCANNNNNTGG 1 cut(s) 404
PfoI TCCNGGA 2 cut(s) 1545, 1657
PkrI GCNGC 5 cut(s) 494, 768, 937, 1068, 1951
PleI GAGTC 3 cut(s) 647, 900, 1810
PpsI GAGTC 3 cut(s) 647, 900, 1810
PshBI ATTAAT 2 cut(s) 129, 1278
Psp124BI GAGCTC 2 cut(s) 1477, 1731
Psp6I CCWGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
PspGI CCWGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
PspLI CGTACG 1 cut(s) 1447
PspN4I GGNNCC 2 cut(s) 140, 1189
PspPI GGNCC 1 cut(s) 1717
PsrI GAACNNNNNNTAC 2 cut(s) 1149, 1181
PsuI RGATCY 3 cut(s) 908, 1592, 1654
RsaI GTAC 8 cut(s) 7, 13, 45, 448, 484, 784, 1189, 1449
RsaNI GTAC 8 cut(s) 6, 12, 44, 447, 483, 783, 1188, 1448
RseI CAYNNNNRTG 2 cut(s) 1314, 1416
SacI GAGCTC 2 cut(s) 1477, 1731
SaqAI TTAA 7 cut(s) 129, 1041, 1107, 1152, 1278, 1560, 1733
SatI GCNGC 5 cut(s) 493, 767, 936, 1067, 1950
Sau3AI GATC 5 cut(s) 514, 908, 1592, 1654, 1872
Sau96I GGNCC 1 cut(s) 1717
ScaI AGTACT 3 cut(s) 7, 448, 784
SchI GAGTC 3 cut(s) 647, 901, 1811
ScrFI CCNGG 7 cut(s) 60, 117, 278, 590, 599, 1547, 1659
SduI GDGCHC 3 cut(s) 672, 1477, 1731
SexAI ACCWGGT 1 cut(s) 115
SfaNI GCATC 4 cut(s) 205, 1624, 1828, 1892
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 1717
SmiMI CAYNNNNRTG 2 cut(s) 1314, 1416
SmlI CTYRAG 2 cut(s) 173, 1732
SmoI CTYRAG 2 cut(s) 173, 1732
SpeI ACTAGT 2 cut(s) 533, 1982
SphI GCATGC 1 cut(s) 196
SseBI AGGCCT 1 cut(s) 948
SsiI CCGC 7 cut(s) 78, 153, 509, 702, 1030, 1469, 1949
SspI AATATT 1 cut(s) 648
SspMI CTAG 7 cut(s) 23, 534, 944, 1652, 1784, 1958, 1983
SstI GAGCTC 2 cut(s) 1477, 1731
StuI AGGCCT 1 cut(s) 948
StyD4I CCNGG 7 cut(s) 58, 115, 276, 588, 597, 1545, 1657
StyI CCWWGG 5 cut(s) 706, 949, 1122, 1174, 1806
TaaI ACNGT 7 cut(s) 473, 722, 984, 1062, 1192, 1272, 1974
TaiI ACGT 2 cut(s) 1701, 1930
TaqI TCGA 5 cut(s) 672, 729, 787, 852, 1519
TatI WGTACW 3 cut(s) 5, 446, 782
TauI GCSGC 1 cut(s) 1952
TfiI GAWTC 1 cut(s) 731
Tru1I TTAA 7 cut(s) 129, 1041, 1107, 1152, 1278, 1560, 1733
Tru9I TTAA 7 cut(s) 129, 1041, 1107, 1152, 1278, 1560, 1733
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 2 cut(s) 238, 1694
TseI GCWGC 4 cut(s) 492, 766, 935, 1066
Tsp45I GTSAC 2 cut(s) 238, 1694
TspGWI ACGGA 1 cut(s) 1700
TspRI CASTG 1 cut(s) 484
Van91I CCANNNNNTGG 1 cut(s) 404
Vha464I CTTAAG 1 cut(s) 1732
VpaK11BI GGWCC 1 cut(s) 1717
VspI ATTAAT 2 cut(s) 129, 1278
XapI RAATTY 6 cut(s) 90, 750, 919, 1373, 1556, 1965
XbaI TCTAGA 1 cut(s) 1651
XceI RCATGY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 2 cut(s) 1770, 1903
XmiI GTMKAC 1 cut(s) 594
XmnI GAANNNNTTC 2 cut(s) 750, 1393
XspI CTAG 7 cut(s) 23, 534, 944, 1652, 1784, 1958, 1983
ZrmI AGTACT 3 cut(s) 7, 448, 784
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.