RchiOBHm_Chr2g0152581

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000052
Physical Location & Seq
Reverse (-)
69959258 .. 69962179
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52173

Sequence Viewer

Length: 2178 bp
ATGGTTGCCCAGTTGCTATTTCATATTACTCTGTTACTATGGTCTATGAGTAGTACTGAAGTACTAGTTGTAGCATCAGAAGCACCCATTGTAGCAACAGAAGCACCCATAGCAAAGCCTAATTGTCCATCGCATTGTGGAGGCATTGAAATCCCATACCCTTTTGGGATAGGAGCAGGTTGTTACATTCATGACGACTGGTTTCAAGTATTCTGTGACAACTCCACTGGCTCTCCTACACCTTTCTTGAACGGCACCAATCTGGAGGTACTAGATATTTCTGTTGAAGGCACTCTAAAGGTCAGAAACCCAATCACCTTCTCTAATTGCACTGATAAGCCAAATCGTCAAGCAGTCAACTTGGAGGGAAGCCCTTTTATGTTCTCCCAGAAGAACATGTTCACTGCAGTAGGTTGCGGAGTTATGGCCACAATCACCTCAAACTCAAATGGCGTTACCATTTCAGCCGGGTGCAGGTCCGAATGTTATGATTATACTGTTAGTAAAAATAGTAGCAGCCATAATACCTGCAATGGTGTGGATTGCTGCCAGACTGTCATCCCTCCTTCCCTCTCTGCCTTCAATACTAGTTTCCAGCAGGCTAATGATAATTACACAAATAGTTCATGTAATTATGCATTTTTGGTTGATCGTGACTGGTTTGAGTCATTTGCCAAAAATTCAACGAATATTTCTGCCACCAGTGATATGGACCAAGTCCCCGTGGAGCTAAAATGGAACTTGTACCATTCAACAACTGATGTTTTTGGGACCTTTAAAGAAACAAATGTATCGGTGCGGTATGCTGATGACCTAGATGGTTATTGCGAGACTTACAACGACAGTTCTTCATCATATGAATCCTTAAGGCTTGAATGTTCCTGTGGAGGGGGCTCTACGGGAAATCCATATCTTCCTCAAGGATGCATAGATATCAATGAATGTGAGGATCCGGAAAACAGAGCTAGTTGTTCTGCAGGGCTCGTATGTGTCAACTATAATAATGGATGGGGATGCACCTACCCAGAAACCAGCAAGTCGCGGGTTAAAGTAATCTTCATAGGTATCGGCAGTGGTTTTGGACTGATAGTTCTACTAATTGGTGCGTGGTGGTTGTACAAACTTTGGAAGAAAAGGAAAAACATTAAACTCAAGAAGAAGTTCTTTAAACAAAATGGGGGACTATTATTAGAGCAGCAACTATCTTCCGGTGAAGTTAATGTTGAGAAAATTAAGCTGTTCAATTCAAAGGAATTAGAAAAAGCCACAGACCGGTTTAATGTAGATAGAATACTCGGCCAAGGAGGCCAAGGTACTGTTTACAAAGGAATGTTGGCAGATGGAAGAATTGTTGCTGTAAAGAAGTCTAAAATAGTTGATGGGGGTGAAACCGAAGTTCCTCTTTTGGTTTACGAATTCATACCCAAAGGGACTATCTACCGGTATCTCCATGAACAGAATGAAGAGTTTCCACTCACATGGGAAATGCGCTTAAGGATTTCTGCAGAAATTGCCGGAGCTCTTTCATACTTACACTCTGCAGCTGCTTTTCCAATTTACCACCGTGATATCAAGTCTACAAACATACTATTAGATGATAAATACAGAGCAAAAGTTGCAGATTTTGGGACTTCAAGATCAGTTTCCATAGACCAAACACACCTGACCACATTTGTACATGGTACATTTGGTTACTTGGATCCAGAGTACTTCCAGTCAAGTCAATTTACAGACAAAAGTGACGTGTATAGCTTTGGAGTGGTCCTTGTCGAGCTCTTGACTGGAGAAAAACCAGTTTCTCTGACTAGGTCACAAGAAGCCAGAGGCCTTGTTTCATATTTCAATCTCTCACTGGAGTCGAATAATTTGTTTGATATTATTGATGCTCGAGTTAAGGTGGAGGGTGTAACAGGAGATATTTTGACAGTAGCTAATCTTGCAAAAAGATGTCTGGATATGAATGGGAAGAGACGACCTACCATGAAAGAAGTTGCAATGGAGTTGGAGGTGATTCATAAATCAGTGAAAACGTCAGATATACAACAAAATCATGAAGAGGTTGAGTATGTCCGAAATGAAGTAACTTGTCCTTGGGATGTTGCTTCTACATCAACCGGGTCAGCTTTGGATGGTGGCACTGCTTCATCAATTGATTCAATACCACTAATGTCCAACTGA

Protein Analysis

725

Amino Acids

80.0

Weight (kDa)

5.22

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 8.2e-11 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 465 - 668 1.4e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 468 - 664 3.1e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 167, 465, 536
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 1577
AccII CGCG 1 cut(s) 1042
AccIII TCCGGA 1 cut(s) 952
AciI CCGC 3 cut(s) 417, 799, 1042
AclWI GGATC 4 cut(s) 944, 957, 1694, 1707
AcoI YGGCCR 2 cut(s) 426, 1297
AcsI RAATTY 2 cut(s) 679, 1415
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 9 cut(s) 55, 63, 270, 746, 1118, 1315, 1677, 1684, 1709
AfiI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
AflII CTTAAG 2 cut(s) 865, 1492
AflIII ACRYGT 2 cut(s) 396, 1743
AgeI ACCGGT 2 cut(s) 1272, 1440
AhlI ACTAGT 2 cut(s) 64, 587
AjiI CACGTC 1 cut(s) 1744
AloI GAACNNNNNNTCC 2 cut(s) 1074, 1106
AluBI AGCT 9 cut(s) 730, 965, 1237, 1520, 1544, 1752, 1774, 1931, 2123
AluI AGCT 9 cut(s) 730, 965, 1237, 1520, 1544, 1752, 1774, 1931, 2123
Alw21I GWGCWC 2 cut(s) 1522, 1776
Alw26I GTCTC 2 cut(s) 824, 1963
AlwI GGATC 4 cut(s) 944, 957, 1694, 1707
Ama87I CYCGRG 1 cut(s) 1887
Aor13HI TCCGGA 1 cut(s) 952
AoxI GGCC 4 cut(s) 426, 1297, 1306, 1825
ApeKI GCWGC 5 cut(s) 516, 546, 1195, 1541, 1544
ApoI RAATTY 2 cut(s) 679, 1415
AsiGI ACCGGT 2 cut(s) 1272, 1440
Asp700I GAANNNNTTC 3 cut(s) 398, 1160, 1467
AspLEI GCGC 1 cut(s) 1491
AspS9I GGNCC 4 cut(s) 477, 712, 771, 1762
AsuC2I CCSGG 2 cut(s) 469, 2116
AsuHPI GGTGA 5 cut(s) 307, 427, 1223, 1397, 2020
AvaI CYCGRG 1 cut(s) 1887
AvaII GGWCC 4 cut(s) 477, 712, 771, 1762
BaeI ACNNNNGTAYC 2 cut(s) 774, 807
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 2 cut(s) 949, 1699
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 4 cut(s) 896, 984, 1522, 1776
Bbv12I GWGCWC 2 cut(s) 1522, 1776
BbvI GCAGC 5 cut(s) 528, 533, 1207, 1531, 1553
BccI CCATC 6 cut(s) 136, 812, 1002, 1334, 1373, 2123
BceAI ACGGC 1 cut(s) 268
BcnI CCSGG 2 cut(s) 469, 2116
BcoDI GTCTC 2 cut(s) 824, 1963
BcuI ACTAGT 2 cut(s) 64, 587
BfaI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1806
BfmI CTRYAG 4 cut(s) 405, 975, 1503, 1539
BfrI CTTAAG 2 cut(s) 865, 1492
BfuAI ACCTGC 3 cut(s) 167, 465, 536
BglI GCCNNNNNGGC 1 cut(s) 1305
BisI GCNGC 5 cut(s) 517, 547, 1196, 1542, 1545
BlsI GCNGC 5 cut(s) 518, 548, 1197, 1543, 1546
BmcAI AGTACT 3 cut(s) 55, 63, 1709
Bme1390I CCNGG 2 cut(s) 469, 2116
Bme18I GGWCC 4 cut(s) 477, 712, 771, 1762
BmeT110I CYCGRG 1 cut(s) 1887
BmgBI CACGTC 1 cut(s) 1744
BmgT120I GGNCC 4 cut(s) 477, 712, 771, 1762
BmiI GGNNCC 4 cut(s) 256, 772, 951, 1701
BmrFI CCNGG 2 cut(s) 469, 2116
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 4 cut(s) 83, 914, 1004, 1873
BmuI ACTGGG 1 cut(s) 4
BpmI CTGGAG 3 cut(s) 284, 1803, 1874
BpuEI CTTGAG 2 cut(s) 903, 1136
BpuMI CCSGG 2 cut(s) 469, 2116
BsaJI CCNNGG 4 cut(s) 723, 1300, 1309, 2090
BsaWI WCCGGW 4 cut(s) 952, 1208, 1272, 1440
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 888, 1272
Bse118I RCCGGY 2 cut(s) 1272, 1440
Bse1I ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1714, 1786, 1793, 1857
Bse3DI GCAATG 2 cut(s) 538, 2001
BseAI TCCGGA 1 cut(s) 952
BseDI CCNNGG 4 cut(s) 723, 1300, 1309, 2090
BseGI GGATG 6 cut(s) 558, 929, 1013, 1019, 2101, 2134
BseLI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
BseMI GCAATG 2 cut(s) 538, 2001
BseNI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1714, 1786, 1793, 1857
BseXI GCAGC 5 cut(s) 528, 533, 1207, 1531, 1553
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 4 cut(s) 428, 1299, 1308, 1827
BshNI GGYRCC 1 cut(s) 254
BshTI ACCGGT 2 cut(s) 1272, 1440
BsiHKAI GWGCWC 2 cut(s) 1522, 1776
BsiHKCI CYCGRG 1 cut(s) 1887
BsiSI CCGG 7 cut(s) 468, 953, 1209, 1273, 1441, 1515, 2115
BslFI GGGAC 5 cut(s) 704, 784, 1194, 1444, 1642
BslI CCNNNNNNNGG 3 cut(s) 474, 888, 1272
BsmAI GTCTC 2 cut(s) 824, 1963
BsmBI CGTCTC 1 cut(s) 1963
BsmFI GGGAC 5 cut(s) 704, 784, 1194, 1444, 1642
BsnI GGCC 4 cut(s) 428, 1299, 1308, 1827
BsoBI CYCGRG 1 cut(s) 1887
Bsp1286I GDGCHC 4 cut(s) 896, 984, 1522, 1776
Bsp13I TCCGGA 1 cut(s) 952
Bsp1407I TGTACA 2 cut(s) 1116, 1675
Bsp143I GATC 4 cut(s) 649, 949, 1637, 1699
BspACI CCGC 3 cut(s) 417, 799, 1042
BspANI GGCC 4 cut(s) 428, 1299, 1308, 1827
BspEI TCCGGA 1 cut(s) 952
BspFNI CGCG 1 cut(s) 1042
BspHI TCATGA 2 cut(s) 190, 2050
BspLI GGNNCC 4 cut(s) 256, 772, 951, 1701
BspMAI CTGCAG 4 cut(s) 409, 979, 1507, 1543
BspMI ACCTGC 3 cut(s) 167, 465, 536
BspPI GGATC 4 cut(s) 944, 957, 1694, 1707
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 2 cut(s) 865, 1492
BsrDI GCAATG 2 cut(s) 538, 2001
BsrFI RCCGGY 2 cut(s) 1272, 1440
BsrGI TGTACA 2 cut(s) 1116, 1675
BsrI ACTGG 9 cut(s) 10, 203, 232, 662, 702, 1714, 1786, 1793, 1857
BssAI RCCGGY 2 cut(s) 1272, 1440
BssECI CCNNGG 4 cut(s) 723, 1300, 1309, 2090
BssMI GATC 4 cut(s) 649, 949, 1637, 1699
BssT1I CCWWGG 3 cut(s) 1300, 1309, 2090
Bst4CI ACNGT 6 cut(s) 499, 556, 845, 1318, 1565, 1927
Bst6I CTCTTC 3 cut(s) 1458, 1961, 2049
BstAFI CTTAAG 2 cut(s) 865, 1492
BstAPI GCANNNNNTGC 2 cut(s) 1511, 1616
BstAUI TGTACA 2 cut(s) 1116, 1675
BstC8I GCNNGC 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 6 cut(s) 558, 929, 1013, 1019, 2101, 2134
BstFNI CGCG 1 cut(s) 1042
BstHHI GCGC 1 cut(s) 1491
BstKTI GATC 4 cut(s) 652, 952, 1640, 1702
BstMAI GTCTC 2 cut(s) 824, 1963
BstMBI GATC 4 cut(s) 649, 949, 1637, 1699
BstMWI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1511, 1616, 1937
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 467, 2114
BstSFI CTRYAG 4 cut(s) 405, 975, 1503, 1539
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 5 cut(s) 528, 533, 1207, 1531, 1553
BstX2I RGATCY 2 cut(s) 949, 1699
BstXI CCANNNNNNTGG 2 cut(s) 709, 1479
BstYI RGATCY 2 cut(s) 949, 1699
BsuRI GGCC 4 cut(s) 428, 1299, 1308, 1827
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1744
BtsCI GGATG 6 cut(s) 558, 929, 1013, 1019, 2101, 2134
BtsI GCAGTG 3 cut(s) 402, 1078, 2136
BtsIMutI CAGTG 8 cut(s) 225, 330, 402, 709, 1078, 1850, 2028, 2136
BveI ACCTGC 3 cut(s) 167, 465, 536
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 2 cut(s) 190, 2050
CfoI GCGC 1 cut(s) 1491
Cfr10I RCCGGY 2 cut(s) 1272, 1440
Cfr13I GGNCC 4 cut(s) 477, 712, 771, 1762
Csp6I GTAC 9 cut(s) 54, 62, 269, 745, 1117, 1314, 1676, 1683, 1708
CspAI ACCGGT 2 cut(s) 1272, 1440
CviAII CATG 8 cut(s) 191, 397, 627, 1451, 1479, 1679, 1981, 2051
CviQI GTAC 9 cut(s) 54, 62, 269, 745, 1117, 1314, 1676, 1683, 1708
DpnI GATC 4 cut(s) 651, 951, 1639, 1701
DpnII GATC 4 cut(s) 649, 949, 1637, 1699
DraI TTTAAA 2 cut(s) 778, 1168
EaeI YGGCCR 2 cut(s) 426, 1297
Eam1104I CTCTTC 3 cut(s) 1458, 1961, 2049
EarI CTCTTC 3 cut(s) 1458, 1961, 2049
Ecl136II GAGCTC 2 cut(s) 1520, 1774
Eco130I CCWWGG 3 cut(s) 1300, 1309, 2090
Eco147I AGGCCT 1 cut(s) 1827
Eco24I GRGCYC 4 cut(s) 896, 984, 1522, 1776
Eco32I GATATC 2 cut(s) 934, 1570
Eco47I GGWCC 4 cut(s) 477, 712, 771, 1762
Eco53kI GAGCTC 2 cut(s) 1520, 1774
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 1887
EcoICRI GAGCTC 2 cut(s) 1520, 1774
EcoO109I RGGNCCY 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 1415
EcoRV GATATC 2 cut(s) 934, 1570
EcoT14I CCWWGG 3 cut(s) 1300, 1309, 2090
EcoT22I ATGCAT 2 cut(s) 640, 929
EcoT38I GRGCYC 4 cut(s) 896, 984, 1522, 1776
ErhI CCWWGG 3 cut(s) 1300, 1309, 2090
Esp3I CGTCTC 1 cut(s) 1963
FaeI CATG 8 cut(s) 194, 400, 630, 1454, 1482, 1682, 1984, 2054
FalI AAGNNNNNCTT 4 cut(s) 1148, 1180, 1386, 1418
FaqI GGGAC 5 cut(s) 704, 784, 1194, 1444, 1642
FatI CATG 8 cut(s) 190, 396, 626, 1450, 1478, 1678, 1980, 2050
FauI CCCGC 1 cut(s) 1035
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 1577
Fnu4HI GCNGC 5 cut(s) 517, 547, 1196, 1542, 1545
FokI GGATG 6 cut(s) 545, 936, 1020, 1026, 2108, 2141
FriOI GRGCYC 4 cut(s) 896, 984, 1522, 1776
Fsp4HI GCNGC 5 cut(s) 517, 547, 1196, 1542, 1545
FspBI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1806
GlaI GCGC 1 cut(s) 1490
GluI GCNGC 5 cut(s) 517, 547, 1196, 1542, 1545
GsuI CTGGAG 3 cut(s) 284, 1803, 1874
HaeIII GGCC 4 cut(s) 428, 1299, 1308, 1827
HapII CCGG 7 cut(s) 468, 953, 1209, 1273, 1441, 1515, 2115
HhaI GCGC 1 cut(s) 1491
Hin1II CATG 8 cut(s) 194, 400, 630, 1454, 1482, 1682, 1984, 2054
Hin6I GCGC 1 cut(s) 1489
HinP1I GCGC 1 cut(s) 1489
HincII GTYRAC 2 cut(s) 358, 994
HindII GTYRAC 2 cut(s) 358, 994
HinfI GANTC 5 cut(s) 665, 860, 1856, 2011, 2153
HpaII CCGG 7 cut(s) 468, 953, 1209, 1273, 1441, 1515, 2115
HphI GGTGA 5 cut(s) 307, 427, 1223, 1397, 2020
Hpy166II GTNNAC 6 cut(s) 358, 402, 994, 1321, 1411, 1578
Hpy188I TCNGA 6 cut(s) 79, 305, 481, 1803, 2035, 2072
Hpy8I GTNNAC 6 cut(s) 358, 402, 994, 1321, 1411, 1578
HpyAV CCTTC 4 cut(s) 281, 328, 576, 589
HpyCH4III ACNGT 6 cut(s) 499, 556, 845, 1318, 1565, 1927
HpyCH4IV ACGT 2 cut(s) 1743, 2030
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1511, 1616, 1937
HpySE526I ACGT 2 cut(s) 1743, 2030
Hsp92II CATG 8 cut(s) 194, 400, 630, 1454, 1482, 1682, 1984, 2054
HspAI GCGC 1 cut(s) 1489
Kpn2I TCCGGA 1 cut(s) 952
Kzo9I GATC 4 cut(s) 649, 949, 1637, 1699
LmnI GCTCC 3 cut(s) 173, 727, 1517
Lsp1109I GCAGC 5 cut(s) 528, 533, 1207, 1531, 1553
LweI GCATC 4 cut(s) 83, 914, 1004, 1873
MaeI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1806
MaeII ACGT 2 cut(s) 1743, 2030
MalI GATC 4 cut(s) 651, 951, 1639, 1701
MboI GATC 4 cut(s) 649, 949, 1637, 1699
MfeI CAATTG 1 cut(s) 2148
MflI RGATCY 2 cut(s) 949, 1699
MhlI GDGCHC 4 cut(s) 896, 984, 1522, 1776
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 674, 1865
MmeI TCCRAC 1 cut(s) 1983
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 2 cut(s) 640, 929
MroI TCCGGA 1 cut(s) 952
MroXI GAANNNNTTC 3 cut(s) 398, 1160, 1467
MscI TGGCCA 1 cut(s) 428
MslI CAYNNNNRTG 1 cut(s) 1477
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 1544
MspCI CTTAAG 2 cut(s) 865, 1492
MspI CCGG 7 cut(s) 468, 953, 1209, 1273, 1441, 1515, 2115
MspR9I CCNGG 2 cut(s) 469, 2116
MunI CAATTG 1 cut(s) 2148
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 7 cut(s) 80, 101, 110, 1305, 1511, 1616, 1937
NciI CCSGG 2 cut(s) 469, 2116
NdeI CATATG 1 cut(s) 856
NdeII GATC 4 cut(s) 649, 949, 1637, 1699
NlaIII CATG 8 cut(s) 194, 400, 630, 1454, 1482, 1682, 1984, 2054
NlaIV GGNNCC 4 cut(s) 256, 772, 951, 1701
NmeAIII GCCGAG 1 cut(s) 1275
NmuCI GTSAC 4 cut(s) 215, 653, 1739, 1809
NsiI ATGCAT 2 cut(s) 640, 929
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 1887
PagI TCATGA 2 cut(s) 190, 2050
PceI AGGCCT 1 cut(s) 1827
PciI ACATGT 1 cut(s) 396
PdmI GAANNNNTTC 3 cut(s) 398, 1160, 1467
PfeI GAWTC 3 cut(s) 860, 2011, 2153
PflFI GACNNNGTC 2 cut(s) 716, 1807
PinAI ACCGGT 2 cut(s) 1272, 1440
PkrI GCNGC 5 cut(s) 518, 548, 1197, 1543, 1546
PleI GAGTC 2 cut(s) 673, 1864
PpsI GAGTC 2 cut(s) 673, 1864
PpuMI RGGWCCY 1 cut(s) 771
PscI ACATGT 1 cut(s) 396
Psp124BI GAGCTC 2 cut(s) 1522, 1776
Psp5II RGGWCCY 1 cut(s) 771
PspN4I GGNNCC 4 cut(s) 256, 772, 951, 1701
PspPI GGNCC 4 cut(s) 477, 712, 771, 1762
PspPPI RGGWCCY 1 cut(s) 771
PspXI VCTCGAGB 1 cut(s) 1887
PstI CTGCAG 4 cut(s) 409, 979, 1507, 1543
PsuI RGATCY 2 cut(s) 949, 1699
PsyI GACNNNGTC 2 cut(s) 716, 1807
PvuII CAGCTG 1 cut(s) 1544
RsaI GTAC 9 cut(s) 55, 63, 270, 746, 1118, 1315, 1677, 1684, 1709
RsaNI GTAC 9 cut(s) 54, 62, 269, 745, 1117, 1314, 1676, 1683, 1708
RseI CAYNNNNRTG 1 cut(s) 1477
SacI GAGCTC 2 cut(s) 1522, 1776
SatI GCNGC 5 cut(s) 517, 547, 1196, 1542, 1545
Sau3AI GATC 4 cut(s) 649, 949, 1637, 1699
Sau96I GGNCC 4 cut(s) 477, 712, 771, 1762
ScaI AGTACT 3 cut(s) 55, 63, 1709
SchI GAGTC 2 cut(s) 674, 1865
ScrFI CCNGG 2 cut(s) 469, 2116
SduI GDGCHC 4 cut(s) 896, 984, 1522, 1776
SfaNI GCATC 4 cut(s) 83, 914, 1004, 1873
SfcI CTRYAG 4 cut(s) 405, 975, 1503, 1539
SfiI GGCCNNNNNGGCC 1 cut(s) 1305
Sfr274I CTCGAG 1 cut(s) 1887
SinI GGWCC 4 cut(s) 477, 712, 771, 1762
SlaI CTCGAG 1 cut(s) 1887
SmiMI CAYNNNNRTG 1 cut(s) 1477
SmlI CTYRAG 5 cut(s) 865, 918, 1151, 1492, 1887
SmoI CTYRAG 5 cut(s) 865, 918, 1151, 1492, 1887
SpeI ACTAGT 2 cut(s) 64, 587
SseBI AGGCCT 1 cut(s) 1827
SsiI CCGC 3 cut(s) 417, 799, 1042
SspI AATATT 1 cut(s) 691
SspMI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1806
SstI GAGCTC 2 cut(s) 1522, 1776
StuI AGGCCT 1 cut(s) 1827
StyD4I CCNGG 2 cut(s) 467, 2114
StyI CCWWGG 3 cut(s) 1300, 1309, 2090
TaaI ACNGT 6 cut(s) 499, 556, 845, 1318, 1565, 1927
TaiI ACGT 2 cut(s) 1746, 2033
TaqI TCGA 3 cut(s) 1770, 1859, 1888
TatI WGTACW 5 cut(s) 53, 61, 1116, 1675, 1707
TfiI GAWTC 3 cut(s) 860, 2011, 2153
TscAI CASTG 8 cut(s) 232, 337, 409, 709, 1078, 1857, 2028, 2143
TseFI GTSAC 4 cut(s) 215, 653, 1739, 1809
TseI GCWGC 5 cut(s) 516, 546, 1195, 1541, 1544
Tsp45I GTSAC 4 cut(s) 215, 653, 1739, 1809
TspRI CASTG 8 cut(s) 232, 337, 409, 709, 1078, 1857, 2028, 2143
Tth111I GACNNNGTC 2 cut(s) 716, 1807
Vha464I CTTAAG 2 cut(s) 865, 1492
VpaK11BI GGWCC 4 cut(s) 477, 712, 771, 1762
XapI RAATTY 2 cut(s) 679, 1415
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 1887
XmiI GTMKAC 1 cut(s) 1577
XmnI GAANNNNTTC 3 cut(s) 398, 1160, 1467
XspI CTAG 6 cut(s) 65, 272, 588, 815, 966, 1806
ZrmI AGTACT 3 cut(s) 55, 63, 1709
Zsp2I ATGCAT 2 cut(s) 640, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.