RchiOBHm_Chr2g0150781

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000052
Physical Location & Seq
Forward (+)
68452494 .. 68455191
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52003

Sequence Viewer

Length: 2262 bp
ATGGAGGTCCAGTTGCTCCTACTTGAGATTACTCTCATGACCTTGTTGTCTTTGAGCTGTAGTGCAATACTTGGCGTAGCATCATCACCCTCGTTAGCAAAGCGTGATTGTCAATCCCGTTGTGGAGGCATAGAAATCCCATATCCTTTTGGGATTGGACCGAATTGTCACCTGCTGAAGAACGACAGTTTTTACGACAAATATACCCGTCTGTTCCAAATATTGTGTGACAACTCCACCAACCCTCCCAAACCTTTCTTGAACCTTACCGACGGTCGACGGGAGATTCTGGAGATATCTCTTGCGGGCACATTAAAAATAAAAAATCCTATCACCTTCTCCGATTGCCCTAGCAAGCCAAACCGCAGCCGGATACCCAACCTAAGTCCAACCCCTTTCTGGTTCTCCGATAGGAACATGTTCACTGCAGTGAGTTGCGGGAGGCTTGCATCTCTGACGTACCTTGGAGGTGGAGCGACAGAAATGGCTGTCTGTTTGTCTATTTGCGATTACAGCAGCATATTAGTAAATAATAGCTGCCGTGGTATCAACTGTTGTCAGACACCCCTTCCTTTTAACATTGGTGCAAACTTCACTACTAGTTTTGGACCGTTGATAATAAATGGTACAAATGGTACCAGTGGAAGAACTATATTAAATGGTACCAGTGGAAGAACTTGTAAATATGCATTCCTCGTAGACCCAGAGTGGTTTACGTCAAATTCAACAAATATTTATGACATTGCTGAGCTGGACGACGTCCCCGTAGTGCTAAATTGGAGGATTCTTGGAGGAATAGATAATTCTACCAAGGATAACGTGAACAGGAATTGCGAATCTGCTGCTGGTCCATGTTCCTGCTTAGAGGGCTATCAAGGAAACCCCTATCTTCGTGGTGGATGTCAAGATATCAATGAATGCGAGGATCCAAATCGCCCTAATATATGTGGTTCCGACAAGATTTGTTCCAATTTTAATGGGACCTTTGTGTGCTACACCCCACTGTCCCCGCAACCTCGGCCATTGCAGCCGATTAAACTGGGCATTATAGTTCTCTGCAGTGTCTTTGGACTGTTGTTTCATATCATTGGCGCATGGTGGTTGCACAAAGTCATAAAGAAAAGGAAAAACATTAAGCGAAAGCAAAAGTTTTTTAGACAGAATGGTGGTTTGTTGTTGGAGCAACAATTATCTTCTGGCGAAGTCAATGTTGAGAAGATTAAGCTGTTTAATGCAAAGGAGTTAGAGAAAGCCACAGACCGATTTAATGTACACAGAATTCTTGGTCAAGGAGGCCAAGGTACTGTTTATAAAGGAATGTTGACAGATGGAAGAAATGTTGCTGTCAAGAAGTCAAAAGCAGTAGATGGAGGAGAAGTGAGTCAATTCATTAATGAGATTGTCATTCTTTCACAAATTAACCACAGGAATGTGGTTAAACTCTTGGGATGTTGTTTAGAGAGCGAAGTTCCCCTATTGGTCTATGAATTTTTACCCAACGGAACCCTTTCTCAATACATTCACAGCCGTGATGAGGATTTTCCTCTCACTTGGGAAATGCGATTACGAGTTGGTACCGAAGTTGCTGGAGCCCTTTCCTACTTACATTCATCTGCTTCAATGCCCATTTACCATCGAGACATTAAGTCTTCAAACATACTCTTGGATGATAAATATAGAGCAAAAGTTGCAGACTTTGGGACATCAAGAACTGTTTCCATTGACAAAACACATCTTACCATGACACATGTAAACGGAACATTTGGTTATTTGGACCCTGAGTACTTCCAGTCTAGCCAATTTACAGACAAGAGCGACGTTTACAGCTTCGGAGTAGTCCTTGCCGAACTCTTGACTGGACAAAAACCAGTTTCTGTAACAAGATCGCAGGAAGGGAAACAAGATGGGAGAAGCCTAGCAACACATTTTATTGTGTCCATGGAGGAGAGTTGTTTGTTTGATATTCTTGATGCTGAAGTTATGAAGGGAGGTAAGAAAGAAGAGATAGTGGCGGTTGCTAATCTTGCAAAACGTTGCTTGAATATGAAAGGAAAGAAGCGTCCAGCTATGAAAGAAATAGCAGTTAAGTTGGAGGAAATTCTAATGTCAAGTAAATCTTCTGATGTCAAACACATTGCAAAGGTTGAACATGTCCAGACGGAAATAGCCGAGGTTTGGGATGTTTCTACATCAACAGGCTCTCATATGGACACCGGTTCTTCATTAGATGGACAACCACTGCTGTCTTTCAAGTCTCGCTGA

Protein Analysis

753

Amino Acids

83.16

Weight (kDa)

8.25

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 107 1.7e-08 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 422 - 693 7.1e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 697 3.3e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AarI CACCTGC 1 cut(s) 180
AasI GACNNNNNNGTC 2 cut(s) 46, 165
AatII GACGTC 1 cut(s) 762
Acc36I ACCTGC 1 cut(s) 180
Acc65I GGTACC 3 cut(s) 635, 662, 1574
AccB1I GGYRCC 3 cut(s) 635, 662, 1574
AccI GTMKAC 2 cut(s) 277, 699
AciI CCGC 5 cut(s) 305, 364, 438, 1010, 2012
AclI AACGTT 1 cut(s) 2032
AclWI GGATC 2 cut(s) 920, 933
AcoI YGGCCR 1 cut(s) 1019
AcsI RAATTY 4 cut(s) 721, 1278, 1487, 2097
AcuI CTGAAG 2 cut(s) 197, 1995
AcyI GRCGYC 1 cut(s) 759
AfaI GTAC 8 cut(s) 461, 628, 637, 664, 1272, 1303, 1576, 1784
AfiI CCNNNNNNNGG 3 cut(s) 399, 1534, 2175
AflIII ACRYGT 3 cut(s) 417, 1747, 2149
AgeI ACCGGT 1 cut(s) 2213
AgsI TTSAA 7 cut(s) 262, 726, 1620, 1653, 2041, 2147, 2251
AhdI GACNNNNNGTC 1 cut(s) 1645
AhlI ACTAGT 1 cut(s) 599
AleI CACNNNNGTG 2 cut(s) 428, 1527
AluBI AGCT 6 cut(s) 57, 537, 751, 1225, 1827, 2066
AluI AGCT 6 cut(s) 57, 537, 751, 1225, 1827, 2066
Alw26I GTCTC 1 cut(s) 1632
AlwI GGATC 2 cut(s) 920, 933
AlwNI CAGNNNCTG 1 cut(s) 1874
AoxI GGCC 2 cut(s) 1019, 1294
ApeKI GCWGC 5 cut(s) 366, 516, 537, 842, 1027
ApoI RAATTY 4 cut(s) 721, 1278, 1487, 2097
AseI ATTAAT 1 cut(s) 1392
AsiGI ACCGGT 1 cut(s) 2213
Asp700I GAANNNNTTC 3 cut(s) 419, 1507, 1714
Asp718I GGTACC 3 cut(s) 635, 662, 1574
AspLEI GCGC 1 cut(s) 1094
AspS9I GGNCC 6 cut(s) 7, 158, 608, 848, 981, 1774
AsuHPI GGTGA 3 cut(s) 78, 161, 325
AvaII GGWCC 6 cut(s) 7, 158, 608, 848, 981, 1774
BaeGI GKGCMC 1 cut(s) 311
BaeI ACNNNNGTAYC 2 cut(s) 1566, 1599
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 3 cut(s) 635, 662, 1574
BanII GRGCYC 1 cut(s) 1594
BbsI GAAGAC 1 cut(s) 1641
BbvI GCAGC 5 cut(s) 378, 524, 528, 829, 1039
BccI CCATC 5 cut(s) 1322, 1361, 1641, 1898, 2222
BceAI ACGGC 2 cut(s) 525, 1512
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BciVI GTATCC 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 1632
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 4 cut(s) 351, 600, 1794, 1916
BfmI CTRYAG 3 cut(s) 58, 426, 1057
BfuAI ACCTGC 1 cut(s) 180
BfuI GTATCC 1 cut(s) 366
BisI GCNGC 5 cut(s) 367, 517, 538, 843, 1028
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 5 cut(s) 368, 518, 539, 844, 1029
BmcAI AGTACT 1 cut(s) 1784
Bme18I GGWCC 6 cut(s) 7, 158, 608, 848, 981, 1774
BmeRI GACNNNNNGTC 1 cut(s) 1645
BmgT120I GGNCC 6 cut(s) 7, 158, 608, 848, 981, 1774
BmiI GGNNCC 9 cut(s) 637, 664, 927, 952, 982, 1504, 1576, 1591, 1776
BmrI ACTGGG 1 cut(s) 1049
BmsI GCATC 3 cut(s) 89, 458, 1960
BmuI ACTGGG 1 cut(s) 1049
BpiI GAAGAC 1 cut(s) 1641
BpmI CTGGAG 2 cut(s) 311, 1608
Bpu1102I GCTNAGC 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 930
BsaHI GRCGYC 1 cut(s) 759
BsaJI CCNNGG 7 cut(s) 463, 541, 810, 1016, 1297, 1938, 2169
BsaWI WCCGGW 1 cut(s) 2213
BsaXI ACNNNNNCTCC 5 cut(s) 26, 229, 259, 1362, 1392
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 1534, 2175
Bse118I RCCGGY 1 cut(s) 2213
Bse1I ACTGG 7 cut(s) 10, 639, 666, 1044, 1789, 1861, 1868
Bse3DI GCAATG 3 cut(s) 741, 1022, 2133
Bse8I GATNNNNATC 1 cut(s) 930
BseDI CCNNGG 7 cut(s) 463, 541, 810, 1016, 1297, 1938, 2169
BseGI GGATG 4 cut(s) 905, 1454, 1672, 2185
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 3 cut(s) 399, 1534, 2175
BseMI GCAATG 3 cut(s) 741, 1022, 2133
BseMII CTCAG 2 cut(s) 738, 1770
BseNI ACTGG 7 cut(s) 10, 639, 666, 1044, 1789, 1861, 1868
BseRI GAGGAG 2 cut(s) 1386, 1958
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 5 cut(s) 378, 524, 528, 829, 1039
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 2 cut(s) 1021, 1296
BshNI GGYRCC 3 cut(s) 635, 662, 1574
BshTI ACCGGT 1 cut(s) 2213
BsiEI CGRYCG 1 cut(s) 277
BsiSI CCGG 2 cut(s) 370, 2214
BslFI GGGAC 4 cut(s) 746, 991, 994, 1714
BslI CCNNNNNNNGG 3 cut(s) 399, 1534, 2175
BsmAI GTCTC 1 cut(s) 1632
BsmFI GGGAC 4 cut(s) 746, 991, 994, 1714
BsmI GAATGC 2 cut(s) 689, 923
BsnI GGCC 2 cut(s) 1021, 1296
Bsp1286I GDGCHC 2 cut(s) 311, 1594
Bsp1407I TGTACA 1 cut(s) 1270
Bsp143I GATC 2 cut(s) 925, 1883
Bsp1720I GCTNAGC 1 cut(s) 747
Bsp19I CCATGG 1 cut(s) 1938
BspACI CCGC 5 cut(s) 305, 364, 438, 1010, 2012
BspANI GGCC 2 cut(s) 1021, 1296
BspCNI CTCAG 2 cut(s) 739, 1771
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 9 cut(s) 637, 664, 927, 952, 982, 1504, 1576, 1591, 1776
BspMAI CTGCAG 2 cut(s) 430, 1061
BspMI ACCTGC 1 cut(s) 180
BspPI GGATC 2 cut(s) 920, 933
BspT107I GGYRCC 3 cut(s) 635, 662, 1574
BsrDI GCAATG 3 cut(s) 741, 1022, 2133
BsrFI RCCGGY 1 cut(s) 2213
BsrGI TGTACA 1 cut(s) 1270
BsrI ACTGG 7 cut(s) 10, 639, 666, 1044, 1789, 1861, 1868
BssAI RCCGGY 1 cut(s) 2213
BssECI CCNNGG 7 cut(s) 463, 541, 810, 1016, 1297, 1938, 2169
BssMI GATC 2 cut(s) 925, 1883
BssNI GRCGYC 1 cut(s) 759
BssT1I CCWWGG 4 cut(s) 463, 810, 1297, 1938
Bst4CI ACNGT 8 cut(s) 188, 275, 554, 612, 1005, 1074, 1306, 1714
Bst6I CTCTTC 1 cut(s) 1995
BstACI GRCGYC 1 cut(s) 759
BstAPI GCANNNNNTGC 1 cut(s) 1688
BstAUI TGTACA 1 cut(s) 1270
BstC8I GCNNGC 3 cut(s) 307, 356, 447
BstDEI CTNAG 4 cut(s) 383, 747, 862, 1779
BstDSI CCRYGG 2 cut(s) 541, 1938
BstF5I GGATG 4 cut(s) 905, 1454, 1672, 2185
BstHHI GCGC 1 cut(s) 1094
BstKTI GATC 2 cut(s) 928, 1886
BstMAI GTCTC 1 cut(s) 1632
BstMBI GATC 2 cut(s) 925, 1883
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 6 cut(s) 513, 867, 1018, 1027, 1688, 2024
BstNSI RCATGY 3 cut(s) 421, 1751, 2153
BstSFI CTRYAG 3 cut(s) 58, 426, 1057
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 5 cut(s) 378, 524, 528, 829, 1039
BstV2I GAAGAC 1 cut(s) 1641
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 2 cut(s) 1021, 1296
BtgI CCRYGG 2 cut(s) 541, 1938
BtsCI GGATG 4 cut(s) 905, 1454, 1672, 2185
BtsI GCAGTG 4 cut(s) 423, 435, 1066, 2237
BtsIMutI CAGTG 7 cut(s) 423, 435, 646, 673, 1001, 1066, 2237
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 3 cut(s) 307, 356, 447
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 1094
Cfr10I RCCGGY 1 cut(s) 2213
Cfr13I GGNCC 6 cut(s) 7, 158, 608, 848, 981, 1774
CseI GACGC 1 cut(s) 2048
Csp6I GTAC 8 cut(s) 460, 627, 636, 663, 1271, 1302, 1575, 1783
CspAI ACCGGT 1 cut(s) 2213
CviAII CATG 8 cut(s) 37, 418, 852, 1095, 1741, 1748, 1939, 2150
CviQI GTAC 8 cut(s) 460, 627, 636, 663, 1271, 1302, 1575, 1783
DdeI CTNAG 4 cut(s) 383, 747, 862, 1779
DpnI GATC 2 cut(s) 927, 1885
DpnII GATC 2 cut(s) 925, 1883
DrdI GACNNNNNNGTC 2 cut(s) 46, 165
DriI GACNNNNNGTC 1 cut(s) 1645
DseDI GACNNNNNNGTC 2 cut(s) 46, 165
EaeI YGGCCR 1 cut(s) 1019
Eam1104I CTCTTC 1 cut(s) 1995
Eam1105I GACNNNNNGTC 1 cut(s) 1645
EarI CTCTTC 1 cut(s) 1995
Eco130I CCWWGG 4 cut(s) 463, 810, 1297, 1938
Eco24I GRGCYC 1 cut(s) 1594
Eco32I GATATC 2 cut(s) 297, 910
Eco47I GGWCC 6 cut(s) 7, 158, 608, 848, 981, 1774
Eco57I CTGAAG 2 cut(s) 197, 1995
EcoO109I RGGNCCY 1 cut(s) 981
EcoRI GAATTC 1 cut(s) 1278
EcoRV GATATC 2 cut(s) 297, 910
EcoT14I CCWWGG 4 cut(s) 463, 810, 1297, 1938
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 1594
ErhI CCWWGG 4 cut(s) 463, 810, 1297, 1938
FaeI CATG 8 cut(s) 40, 421, 855, 1098, 1744, 1751, 1942, 2153
FalI AAGNNNNNCTT 2 cut(s) 2101, 2133
FaqI GGGAC 4 cut(s) 746, 991, 994, 1714
FatI CATG 8 cut(s) 36, 417, 851, 1094, 1740, 1747, 1938, 2149
FauI CCCGC 3 cut(s) 298, 431, 1017
FauNDI CATATG 1 cut(s) 2205
FblI GTMKAC 2 cut(s) 277, 699
Fnu4HI GCNGC 5 cut(s) 367, 517, 538, 843, 1028
FokI GGATG 4 cut(s) 912, 1461, 1679, 2192
FriOI GRGCYC 1 cut(s) 1594
Fsp4HI GCNGC 5 cut(s) 367, 517, 538, 843, 1028
FspBI CTAG 4 cut(s) 351, 600, 1794, 1916
GlaI GCGC 1 cut(s) 1093
GluI GCNGC 5 cut(s) 367, 517, 538, 843, 1028
GsuI CTGGAG 2 cut(s) 311, 1608
HaeIII GGCC 2 cut(s) 1021, 1296
HapII CCGG 2 cut(s) 370, 2214
HgaI GACGC 1 cut(s) 2048
HhaI GCGC 1 cut(s) 1094
Hin1I GRCGYC 1 cut(s) 759
Hin1II CATG 8 cut(s) 40, 421, 855, 1098, 1744, 1751, 1942, 2153
Hin6I GCGC 1 cut(s) 1092
HinP1I GCGC 1 cut(s) 1092
HincII GTYRAC 2 cut(s) 278, 1323
HindII GTYRAC 2 cut(s) 278, 1323
HinfI GANTC 4 cut(s) 286, 784, 836, 1381
HpaII CCGG 2 cut(s) 370, 2214
HphI GGTGA 3 cut(s) 78, 161, 325
Hpy166II GTNNAC 9 cut(s) 278, 423, 700, 714, 823, 1273, 1323, 1753, 1822
Hpy188I TCNGA 7 cut(s) 343, 409, 456, 561, 955, 1832, 2122
Hpy8I GTNNAC 9 cut(s) 278, 423, 700, 714, 823, 1273, 1323, 1753, 1822
Hpy99I CGWCG 4 cut(s) 275, 282, 761, 1820
HpyAV CCTTC 4 cut(s) 346, 578, 1886, 1978
HpyCH4III ACNGT 8 cut(s) 188, 275, 554, 612, 1005, 1074, 1306, 1714
HpyCH4IV ACGT 6 cut(s) 458, 716, 759, 819, 1818, 2032
HpyF10VI GCNNNNNNNGC 6 cut(s) 513, 867, 1018, 1027, 1688, 2024
HpyF3I CTNAG 4 cut(s) 383, 747, 862, 1779
HpySE526I ACGT 6 cut(s) 458, 716, 759, 819, 1818, 2032
Hsp92I GRCGYC 1 cut(s) 759
Hsp92II CATG 8 cut(s) 40, 421, 855, 1098, 1744, 1751, 1942, 2153
HspAI GCGC 1 cut(s) 1092
KpnI GGTACC 3 cut(s) 639, 666, 1578
Kzo9I GATC 2 cut(s) 925, 1883
LmnI GCTCC 4 cut(s) 21, 473, 1180, 1589
Lsp1109I GCAGC 5 cut(s) 378, 524, 528, 829, 1039
LweI GCATC 3 cut(s) 89, 458, 1960
MaeI CTAG 4 cut(s) 351, 600, 1794, 1916
MaeII ACGT 6 cut(s) 458, 716, 759, 819, 1818, 2032
MaeIII GTNAC 3 cut(s) 167, 227, 1876
MalI GATC 2 cut(s) 927, 1885
MboI GATC 2 cut(s) 925, 1883
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 2 cut(s) 311, 1594
MlyI GAGTC 1 cut(s) 1390
MmeI TCCRAC 4 cut(s) 413, 978, 1158, 2070
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 3 cut(s) 419, 1507, 1714
MslI CAYNNNNRTG 3 cut(s) 428, 1428, 1527
MspI CCGG 2 cut(s) 370, 2214
Mva1269I GAATGC 2 cut(s) 689, 923
MwoI GCNNNNNNNGC 6 cut(s) 513, 867, 1018, 1027, 1688, 2024
NcoI CCATGG 1 cut(s) 1938
NdeI CATATG 1 cut(s) 2205
NdeII GATC 2 cut(s) 925, 1883
NlaIII CATG 8 cut(s) 40, 421, 855, 1098, 1744, 1751, 1942, 2153
NlaIV GGNNCC 9 cut(s) 637, 664, 927, 952, 982, 1504, 1576, 1591, 1776
NmeAIII GCCGAG 2 cut(s) 997, 2194
NmuCI GTSAC 2 cut(s) 167, 227
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 3 cut(s) 421, 1751, 2153
OliI CACNNNNGTG 2 cut(s) 428, 1527
PagI TCATGA 1 cut(s) 36
PaqCI CACCTGC 1 cut(s) 180
PciI ACATGT 3 cut(s) 417, 1747, 2149
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 2 cut(s) 689, 923
PdmI GAANNNNTTC 3 cut(s) 419, 1507, 1714
PfeI GAWTC 3 cut(s) 286, 784, 836
PflFI GACNNNGTC 1 cut(s) 758
PinAI ACCGGT 1 cut(s) 2213
PkrI GCNGC 5 cut(s) 368, 518, 539, 844, 1029
PleI GAGTC 1 cut(s) 1389
PpsI GAGTC 1 cut(s) 1389
PpuMI RGGWCCY 1 cut(s) 981
PscI ACATGT 3 cut(s) 417, 1747, 2149
PshBI ATTAAT 1 cut(s) 1392
PsiI TTATAA 1 cut(s) 1311
Psp1406I AACGTT 1 cut(s) 2032
Psp5II RGGWCCY 1 cut(s) 981
PspN4I GGNNCC 9 cut(s) 637, 664, 927, 952, 982, 1504, 1576, 1591, 1776
PspPI GGNCC 6 cut(s) 7, 158, 608, 848, 981, 1774
PspPPI RGGWCCY 1 cut(s) 981
PstI CTGCAG 2 cut(s) 430, 1061
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 1 cut(s) 925
PsyI GACNNNGTC 1 cut(s) 758
RsaI GTAC 8 cut(s) 461, 628, 637, 664, 1272, 1303, 1576, 1784
RsaNI GTAC 8 cut(s) 460, 627, 636, 663, 1271, 1302, 1575, 1783
RseI CAYNNNNRTG 3 cut(s) 428, 1428, 1527
SalI GTCGAC 1 cut(s) 276
SatI GCNGC 5 cut(s) 367, 517, 538, 843, 1028
Sau3AI GATC 2 cut(s) 925, 1883
Sau96I GGNCC 6 cut(s) 7, 158, 608, 848, 981, 1774
ScaI AGTACT 1 cut(s) 1784
SchI GAGTC 1 cut(s) 1390
SduI GDGCHC 2 cut(s) 311, 1594
SfaNI GCATC 3 cut(s) 89, 458, 1960
SfcI CTRYAG 3 cut(s) 58, 426, 1057
SinI GGWCC 6 cut(s) 7, 158, 608, 848, 981, 1774
SmiMI CAYNNNNRTG 3 cut(s) 428, 1428, 1527
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SpeI ACTAGT 1 cut(s) 599
SsiI CCGC 5 cut(s) 305, 364, 438, 1010, 2012
SspI AATATT 2 cut(s) 222, 733
SspMI CTAG 4 cut(s) 351, 600, 1794, 1916
StyI CCWWGG 4 cut(s) 463, 810, 1297, 1938
TaaI ACNGT 8 cut(s) 188, 275, 554, 612, 1005, 1074, 1306, 1714
TaiI ACGT 6 cut(s) 461, 719, 762, 822, 1821, 2035
TaqI TCGA 2 cut(s) 277, 1636
TaqII GACCGA 2 cut(s) 175, 1275
TatI WGTACW 2 cut(s) 1270, 1782
TfiI GAWTC 3 cut(s) 286, 784, 836
TscAI CASTG 7 cut(s) 430, 435, 646, 673, 1008, 1066, 2244
TseFI GTSAC 2 cut(s) 167, 227
TseI GCWGC 5 cut(s) 366, 516, 537, 842, 1027
Tsp45I GTSAC 2 cut(s) 167, 227
TspDTI ATGAA 9 cut(s) 930, 1070, 1378, 1500, 1599, 1997, 2060, 2084, 2211
TspGWI ACGGA 3 cut(s) 1515, 1770, 2174
TspRI CASTG 7 cut(s) 430, 435, 646, 673, 1008, 1066, 2244
Tth111I GACNNNGTC 1 cut(s) 758
VpaK11BI GGWCC 6 cut(s) 7, 158, 608, 848, 981, 1774
VspI ATTAAT 1 cut(s) 1392
XapI RAATTY 4 cut(s) 721, 1278, 1487, 2097
XceI RCATGY 3 cut(s) 421, 1751, 2153
XcmI CCANNNNNNNNNTGG 1 cut(s) 396
XmiI GTMKAC 2 cut(s) 277, 699
XmnI GAANNNNTTC 3 cut(s) 419, 1507, 1714
XspI CTAG 4 cut(s) 351, 600, 1794, 1916
ZraI GACGTC 1 cut(s) 760
ZrmI AGTACT 1 cut(s) 1784
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.