RchiOBHm_Chr7g0221871

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000052
Physical Location & Seq
Forward (+)
42971173 .. 42986634
15462 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19853

Sequence Viewer

Length: 2328 bp
ATGCAATGGCTAGTTCAGCACATGATTTTCATTCTATTGTTATATAAATGGCGCATATGTACAGCATCATTATCGCCACCGAAGATAGCAAAGGAGAATTGTACATCGCGGTGCGGAGGTGTTAGCATCCCATACCCTTTTGGGATTGGACCTAAAAGCCATTGTTATTTTGACGAGTGGTACGAATTAGAATGCAACCTTTCTGATCCAGTAGCGAAGCCGTTCTTGAAGGGTCTACAACTGGAGGTGCTGACTATTTTTGTTGAAAACTCCACGCTTCAGGTTACAAGCCCTATCACTTACTTTAGCTGCAAGGGGAAGCAATCTCGGCCAGCGGCAAATCTGACGGGAAGCCCTTTTCAGTACTCTGTTTTTAACAGTTTCGTTGCAGTCAGTTGTGGCTTCCTTGTCTCGGTTCTCTCAGGTTCAAATCATACTCTTGGTGGGTGCACATCGACGTGTGGATCAGATCGAAGCAGAGGCTTGTGTTTCGTTGGCGACAACTGTTGCGAGATTGTGATTTCTACAGATCTTATTGCTAATTTCAGTGCTTTCAGACAAGAACAAGATGAAGTAAGAACAAATGCGACGGATTGCGAGGACTGTGCATTCCTGGTTTACGATAAATGGTTTGATAACAATGTATCAGACTCTGACGGATTAGACTTAACAGCTATAAAGGGTATGGAAGTCGTTCCCGTCGAGTTAGAGTGGAGCTTAAGCTTAGCAAAAAATAACTCATTAATCAAATCATTTGAAGCACTCGACCGCTTCCCAGAAGTGAGGAGGCCGAATGACCCAACACCCTCATGCATAGTTAGTTTCGATGATAGTTCGTTTCGATGGTATCAATGTAGTTGTCCGGCAGGCTTTCAAGGAAATCCCTACCTTCTCCGACCTTGTCAAGATATTGATGAATGCAAGGATACTAACCCATGCGTGGGTTCCGATCTTAATACCACTAATATATGGAATATTAGTGATGCGAAATGTCAGAACACTATCGGGGGTCATGCTTGCTACTCAAATAGAACGGGGCAAACCTGCGAATTGTTTGGCGAGAATACCAAAGCTAGGTGCTTTTATACAAGCAGACATCACTCTCAGCTCAAACCTATTCTCTCTGGTCTTGGTGCTAGTATTGGATTATTGGTATTTCTTAGTGCTGCATGGTTGGTATACAAAGTTGCAAAGAAATGGAAGAGCACTAAACGTAAGGAGATGTTTTATAAACAAAATGGTGGTTTATTGTTAGAACAACAATTATCATCAAGTGAAATTAATGTCGAGAGAATCAAAGTATTCAAATCCAAGGAGTTACAGAGTTCTACAGACAATTTCAATGTTGATAGAATTATTGGCCAAGGGGGACAAGGTACTGTATATAAAGGCATGTTGACGGATGGAAGAATTGTGGCTGTTAAAAAATCCAAAATGATAGATAATGCAAATCTTTCAGAATTCATCAATGAGGTTGTCATTCTTTCCCAAATCAACCATAGAAACATCGTTCAACTCTTGGGTTGTTGTTTGGAGACGGAAGTTCCTCTTTTGGTATATGAATTTATCCCTAATGGAAACTTGTTTCAGTATATTCATGGGCAGACCAAAGAGTTTCCACTTACATGGGAGATTCGTTTACGAATTGCTACAGAAATTGCAGGAGCTCTTTCCTACTTACATACATCAGCTTCGTTTCCCATTTATCACAGAGACATCAAGTCCACGAACATACTCTTAGATGACAAATACAGAGCAAAAATTGCAGACTTTGGAACTTCAAGATTAGTTGCCATTGACCAAACTCACCTGACCACAAATGTACATGGCACATTTGGTTATTTGGATCCTGAGTACTACCAATCCAGCCAATTTACTGAGAAAAGTGATGTCTACAGCTTTGGAGTGGTGCTTGTTGAGCTCTTGACCGGAAAAAAACCAATAACAAGGTCAGCCGAAGACGAAATGTACAAAAGTCTTGCCACATATTTTATTATTTCACTGCAGGAAGACTCTCTATTTGACATTCTTGATGCTCGCGTTGTGAATGAAGGCTCTAAAGATGGTATTATGGTAGTCGCTACTCTTGCAAAACGATGTTTAAATCTGAATGGAAGAAAGCGCCCCACGATGAGAGAGATCACAGCAGAGTTGGAGGCCGTCCAATTATCGGAGAAACTTGCCAACGCTCATCAACAAAGTTGTGAAAGGTTTGAATTTGGCCAAGATTATCGAATCGAGCAGGGGGATGACGTTGTTTCTAGTTCAACAATGTCAACTTGGGAAGGTGCTCCACCTCCATCATCAGTTGAACTTCCACTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

775

Amino Acids

86.67

Weight (kDa)

5.62

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 96 1.9e-11 Wall-associated receptor kinase galacturonan-binding
cEGF PF12662 283 - 306 4.2e-06 Complement Clr-like EGF-like
Pkinase PF00069 447 - 715 3.3e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 449 - 718 8.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16090 AT1G16110 AT1G16120 AT1G16130 AT1G16150 AT1G16160 AT1G16260 AT1G16260 AT1G16260 AT1G79670 AT1G79670
fragaria_vesca FvH4_1g22960 FvH4_3g09860 FvH4_3g10284 FvH4_3g10284 FvH4_3g10540 FvH4_3g24040 FvH4_3g35290 FvH4_3g35290 FvH4_5g06860 FvH4_5g06940 FvH4_6g15920 FvH4_6g15920 FvH4_6g37660 FvH4_6g37670 FvH4_6g37691 FvH4_6g37700 FvH4_6g37710 FvH4_6g38990 FvH4_6g39000 FvH4_6g39020 FvH4_6g39020 FvH4_6g39020 FvH4_6g39021 FvH4_6g39030 FvH4_6g39030 FvH4_6g39031 FvH4_6g39032 FvH4_6g45200 FvH4_6g45200
malus_domestica MD08G1107900.v1.1 MD09G1145100.v1.1 MD09G1145200.v1.1 MD09G1145300.v1.1 MD09G1145400.v1.1 MD09G1145500.v1.1 MD17G1131600.v1.1 MD17G1131700.v1.1 MD17G1131900.v1.1 MD17G1132000.v1.1
prunus_persica Prupe.1G188400_v2.0.a1 Prupe.1G188700_v2.0.a1 Prupe.3G180500_v2.0.a1 Prupe.3G180600_v2.0.a1 Prupe.3G180700_v2.0.a1 Prupe.3G181000_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181100_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181200_v2.0.a1 Prupe.3G181400_v2.0.a1 Prupe.3G181500_v2.0.a1 Prupe.3G181600_v2.0.a1 Prupe.3G181800_v2.0.a1 Prupe.3G181900_v2.0.a1 Prupe.3G182000_v2.0.a1 Prupe.3G182100_v2.0.a1 Prupe.3G182200_v2.0.a1 Prupe.3G182300_v2.0.a1 Prupe.3G182400_v2.0.a1 Prupe.3G182500_v2.0.a1 Prupe.3G182600_v2.0.a1 Prupe.3G182700_v2.0.a1 Prupe.5G051200_v2.0.a1 Prupe.5G051300_v2.0.a1 Prupe.5G051400_v2.0.a1 Prupe.5G051500_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051600_v2.0.a1 Prupe.5G051800_v2.0.a1
pyrus_communis pycom09g06470 pycom09g06490 pycom09g06510 pycom09g06520 pycom09g06570 pycom09g06580 pycom17g12440 pycom17g12450 pycom17g12530
rosa_chinensis RchiOBHm_Chr2g0117481 RchiOBHm_Chr2g0128321 RchiOBHm_Chr2g0128331 RchiOBHm_Chr2g0128581 RchiOBHm_Chr2g0128591 RchiOBHm_Chr2g0150651 RchiOBHm_Chr2g0150661 RchiOBHm_Chr2g0150741 RchiOBHm_Chr2g0150781 RchiOBHm_Chr2g0152581 RchiOBHm_Chr2g0152591 RchiOBHm_Chr2g0152641 RchiOBHm_Chr2g0152661 RchiOBHm_Chr2g0152711 RchiOBHm_Chr2g0152731 RchiOBHm_Chr2g0152871 RchiOBHm_Chr2g0152881 RchiOBHm_Chr2g0152901 RchiOBHm_Chr2g0152921 RchiOBHm_Chr2g0152931 RchiOBHm_Chr2g0152941 RchiOBHm_Chr3g0469621 RchiOBHm_Chr3g0469631 RchiOBHm_Chr5g0015591 RchiOBHm_Chr5g0046061 RchiOBHm_Chr5g0047341 RchiOBHm_Chr5g0063341 RchiOBHm_Chr5g0063351 RchiOBHm_Chr6g0264381 RchiOBHm_Chr7g0191741 RchiOBHm_Chr7g0210751 RchiOBHm_Chr7g0221871 RchiOBHm_Chr7g0225671 RchiOBHm_Chr7g0225891 RchiOBHm_Chr7g0225901 RchiOBHm_Chr7g0225961 RchiOBHm_Chr7g0225971 RchiOBHm_Chr7g0234031 RchiOBHm_Chr7g0234041
rosa_laevigata RLG00000001780 RLG00000001784 RLG00000001785 RLG00000001788 RLG00000001789 RLG00000001793 RLG00000001794 RLG00000002941 RLG00000004430 RLG00000014259 RLG00000018349 RLG00000019001 RLG00000019005 RLG00000019011 RLG00000020503 RLG00000020507 RLG00000020648 RLG00000020649 RLG00000020651 RLG00000020652 RLG00000020653 RLG00000020655 RLG00000020656 RLG00000020657 RLG00000023668 RLG00000024331 RLG00000034386 RLG00000035624 RLG00000035625
rosa_multiflora Rmu_co8401977.1_g000001 Rmu_co8409989.1_g000001 Rmu_co8432067.1_g000001 Rmu_co8491011.1_g000001 Rmu_sc0000580.1_g000085 Rmu_sc0000740.1_g000012 Rmu_sc0000740.1_g000014 Rmu_sc0000837.1_g000041 Rmu_sc0000837.1_g000077 Rmu_sc0001552.1_g000008 Rmu_sc0001617.1_g000001 Rmu_sc0002082.1_g000006 Rmu_sc0002082.1_g000007 Rmu_sc0002082.1_g000022 Rmu_sc0002989.1_g000013 Rmu_sc0003064.1_g000011 Rmu_sc0003127.1_g000003 Rmu_sc0003127.1_g000014 Rmu_sc0003127.1_g000028 Rmu_sc0003127.1_g000032 Rmu_sc0003127.1_g000034 Rmu_sc0003127.1_g000035 Rmu_sc0003127.1_g000054 Rmu_sc0003127.1_g000055 Rmu_sc0003465.1_g000034 Rmu_sc0005223.1_g000021 Rmu_sc0005302.1_g000004 Rmu_sc0005378.1_g000005 Rmu_sc0005378.1_g000007 Rmu_sc0005378.1_g000017 Rmu_sc0005378.1_g000034 Rmu_sc0005378.1_g000039 Rmu_sc0005378.1_g000042 Rmu_sc0005378.1_g000056 Rmu_sc0005378.1_g000057 Rmu_sc0005378.1_g000058 Rmu_sc0006483.1_g000013 Rmu_sc0008775.1_g000006 Rmu_sc0008775.1_g000011 Rmu_sc0009104.1_g000010 Rmu_sc0009104.1_g000011 Rmu_sc0009104.1_g000020 Rmu_sc0010960.1_g000006 Rmu_sc0011737.1_g000002 Rmu_sc0011737.1_g000004 Rmu_sc0012875.1_g000003 Rmu_sc0013122.1_g000002 Rmu_sc0018944.1_g000002 Rmu_sc0020155.1_g000001 Rmu_sc0020156.1_g000001 Rmu_ssc0000233.1_g000012 Rmu_ssc0000233.1_g000013 Rmu_ssc0000233.1_g000030 Rmu_ssc0000233.1_g000044 Rmu_ssc0000233.1_g000047 Rmu_ssc0000233.1_g000052 Rmu_ssc0000233.1_g000054 Rmu_ssc0000233.1_g000055 Rmu_ssc0000233.1_g000080 Rmu_ssc0000233.1_g000082 Rmu_ssc0000233.1_g000087 Rmu_ssc0000233.1_g000094 Rmu_ssc0000480.1_g000060 Rmu_ssc0000480.1_g000061
rosa_roxburghii Rroxscaffold_1G00017360 Rroxscaffold_1G00017370 Rroxscaffold_1G00060880 Rroxscaffold_2G00095370 Rroxscaffold_2G00095420 Rroxscaffold_2G00095450 Rroxscaffold_2G00095460 Rroxscaffold_2G00095490 Rroxscaffold_2G00095560 Rroxscaffold_2G00095570 Rroxscaffold_2G00096960 Rroxscaffold_2G00097010 Rroxscaffold_2G00126500 Rroxscaffold_3G00233390 Rroxscaffold_3G00233580 Rroxscaffold_3G00263590 Rroxscaffold_6G00411660
rosa_rugosa Rorug01G0142700.1 Rorug01G0148600.1 Rorug02G0212800 Rorug02G0422400 Rorug02G0434700 Rorug02G0434800 Rorug02G0435000 Rorug02G0435200 Rorug02G0435300 Rorug02G0435400 Rorug02G0435700 Rorug02G0435800 Rorug02G0436200 Rorug02G0436300 Rorug03G0102800 Rorug04G0123600 Rorug04G0123700 Rorug04G0123800 Rorug04G0124000 Rorug04G0124000 Rorug04G0124500 Rorug04G0124700 Rorug04G0124900 Rorug05G0028600.1 Rorug05G0356100 Rorug05G0356800 Rorug06G0011900 Rorug07G0092900 Rorug07G0122800 Rorug07G0232500 Rorug07G0232600 Rorug07G0232700
rosa_samantha Rh1AG408900 Rh2AG332700 Rh2AG333400 Rh2AG482100 Rh2AG482200 Rh2AG482500 Rh2AG496000 Rh2AG496100 Rh2AG496200 Rh2AG496300 Rh2AG497100 Rh2AG497200 Rh2AG497400 Rh2BG280300 Rh2BG340500 Rh2BG494400 Rh2BG494900 Rh2BG507800 Rh2BG507900 Rh2BG508000 Rh2BG508100 Rh2BG508300 Rh2BG508500 Rh2BG508700 Rh2BG509200 Rh2BG512700 Rh2CG271900 Rh2CG319100 Rh2CG319400 Rh2CG357200 Rh2CG468400 Rh2CG468500 Rh2CG468800 Rh2CG482200 Rh2CG482300 Rh2CG482400 Rh2CG482500 Rh2CG483200 Rh2CG483300 Rh2CG483500 Rh2CG483600 Rh2DG294600 Rh2DG357100 Rh2DG357700 Rh2DG503400 Rh2DG504300 Rh2DG504900 Rh2DG505500 Rh2DG505900 Rh2DG519000 Rh2DG519100 Rh2DG519300 Rh2DG519400 Rh2DG520000 Rh2DG520200 Rh2DG520500 Rh2DG521000 Rh2DG524300 Rh3AG152400 Rh3AG334400 Rh3BG176100 Rh3CG167200 Rh3DG199400 Rh3DG199500 Rh5AG415900 Rh5BG430800 Rh5CG454300 Rh5DG444600 Rh6AG247500 Rh6AG247700 Rh7AG120600 Rh7AG369800 Rh7AG370800 Rh7AG468300 Rh7BG366400 Rh7CG125200 Rh7CG388700 Rh7CG389400 Rh7CG459600 Rh7DG123300 Rh7DG123400 Rh7DG123800 Rh7DG123900 Rh7DG377200
rosa_wichuraiana Rw0G004850 Rw0G004870 Rw0G013120 Rw1G013520 Rw2G026850 Rw2G026910 Rw2G027120 Rw2G039340 Rw2G039360 Rw2G039410 Rw2G039420 Rw2G039440 Rw2G039520 Rw2G040810 Rw2G040830 Rw2G040850 Rw2G040860 Rw2G040880 Rw2G041210 Rw2G041220 Rw2G041240 Rw2G041250 Rw3G014370 Rw5G010520 Rw5G039070 Rw6G021410 Rw6G021420 Rw7G010280 Rw7G021780 Rw7G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc36I ACCTGC 1 cut(s) 1052
AccI GTMKAC 3 cut(s) 235, 1179, 1893
AccII CGCG 2 cut(s) 109, 2040
AciI CCGC 4 cut(s) 109, 114, 335, 769
AclWI GGATC 4 cut(s) 200, 472, 1841, 1854
AcoI YGGCCR 3 cut(s) 329, 1360, 2221
AcsI RAATTY 3 cut(s) 1460, 1562, 2216
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 8 cut(s) 61, 103, 182, 365, 1378, 1824, 1856, 1970
AfiI CCNNNNNNNGG 5 cut(s) 412, 940, 941, 1074, 2170
AflII CTTAAG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 458
AhdI GACNNNNNGTC 1 cut(s) 1720
AjiI CACGTC 1 cut(s) 459
AjnI CCWGG 1 cut(s) 612
Alw21I GWGCWC 5 cut(s) 452, 1208, 1669, 1923, 2293
Alw26I GTCTC 3 cut(s) 415, 1529, 1707
Alw44I GTGCAC 1 cut(s) 448
AlwI GGATC 4 cut(s) 200, 472, 1841, 1854
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 5 cut(s) 329, 788, 1360, 2157, 2221
ApaLI GTGCAC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 309, 1166
ApoI RAATTY 3 cut(s) 1460, 1562, 2216
ArsI GACNNNNNNTTYG 2 cut(s) 2003, 2035
AseI ATTAAT 2 cut(s) 743, 1281
Asp700I GAANNNNTTC 2 cut(s) 221, 693
AspLEI GCGC 2 cut(s) 54, 2124
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 1799
AvaII GGWCC 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 452
BalI TGGCCA 2 cut(s) 1362, 2223
BamHI GGATCC 1 cut(s) 1846
BanII GRGCYC 2 cut(s) 1669, 1923
BbsI GAAGAC 2 cut(s) 1965, 2016
Bbv12I GWGCWC 5 cut(s) 452, 1208, 1669, 1923, 2293
BbvI GCAGC 2 cut(s) 296, 1153
BccI CCATC 4 cut(s) 837, 1397, 2057, 2308
BceAI ACGGC 2 cut(s) 205, 2144
BcgI CGANNNNNNTGC 4 cut(s) 54, 88, 587, 621
BciT130I CCWGG 1 cut(s) 614
BciVI GTATCC 1 cut(s) 919
BcoDI GTCTC 3 cut(s) 415, 1529, 1707
BfaI CTAG 4 cut(s) 11, 1074, 1137, 2262
BfmI CTRYAG 5 cut(s) 525, 1329, 1650, 1894, 2003
BfoI RGCGCY 1 cut(s) 2125
BfrI CTTAAG 1 cut(s) 718
BfuAI ACCTGC 1 cut(s) 1052
BfuI GTATCC 1 cut(s) 919
BglII AGATCT 1 cut(s) 529
BisI GCNGC 3 cut(s) 310, 336, 1167
BlpI GCTNAGC 1 cut(s) 724
BlsI GCNGC 3 cut(s) 311, 337, 1168
BmcAI AGTACT 2 cut(s) 365, 1856
Bme1390I CCNGG 1 cut(s) 614
Bme18I GGWCC 1 cut(s) 149
BmeRI GACNNNNNGTC 1 cut(s) 1720
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 2 cut(s) 946, 1848
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 4 cut(s) 74, 135, 973, 2023
BpiI GAAGAC 2 cut(s) 1965, 2016
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 724
BsaJI CCNNGG 2 cut(s) 1311, 1363
BsaWI WCCGGW 1 cut(s) 1928
Bsc4I CCNNNNNNNGG 5 cut(s) 412, 940, 941, 1074, 2170
Bse1I ACTGG 2 cut(s) 209, 246
Bse3DI GCAATG 1 cut(s) 11
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 2 cut(s) 1311, 1363
BseGI GGATG 3 cut(s) 126, 1408, 2254
BseLI CCNNNNNNNGG 5 cut(s) 412, 940, 941, 1074, 2170
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 4 cut(s) 435, 1118, 1842, 1869
BseNI ACTGG 2 cut(s) 209, 246
BseRI GAGGAG 1 cut(s) 799
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 2 cut(s) 296, 1153
Bsh1236I CGCG 2 cut(s) 109, 2040
Bsh1285I CGRYCG 1 cut(s) 769
BshFI GGCC 5 cut(s) 331, 790, 1362, 2159, 2223
BsiEI CGRYCG 1 cut(s) 769
BsiHKAI GWGCWC 5 cut(s) 452, 1208, 1669, 1923, 2293
BsiSI CCGG 2 cut(s) 863, 1929
BslFI GGGAC 1 cut(s) 1383
BslI CCNNNNNNNGG 5 cut(s) 412, 940, 941, 1074, 2170
BsmAI GTCTC 3 cut(s) 415, 1529, 1707
BsmBI CGTCTC 1 cut(s) 1529
BsmFI GGGAC 1 cut(s) 1383
BsmI GAATGC 3 cut(s) 197, 608, 923
BsnI GGCC 5 cut(s) 331, 790, 1362, 2159, 2223
Bsp1286I GDGCHC 5 cut(s) 452, 1208, 1669, 1923, 2293
Bsp1407I TGTACA 4 cut(s) 59, 101, 1822, 1968
Bsp143I GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
Bsp1720I GCTNAGC 1 cut(s) 724
BspACI CCGC 4 cut(s) 109, 114, 335, 769
BspANI GGCC 5 cut(s) 331, 790, 1362, 2159, 2223
BspCNI CTCAG 4 cut(s) 434, 1117, 1843, 1870
BspFNI CGCG 2 cut(s) 109, 2040
BspLI GGNNCC 2 cut(s) 946, 1848
BspMAI CTGCAG 1 cut(s) 2007
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 4 cut(s) 200, 472, 1841, 1854
BspQI GCTCTTC 1 cut(s) 1196
BspTI CTTAAG 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 11
BsrGI TGTACA 4 cut(s) 59, 101, 1822, 1968
BsrI ACTGG 2 cut(s) 209, 246
BssECI CCNNGG 2 cut(s) 1311, 1363
BssMI GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
BssNAI GTATAC 1 cut(s) 1180
BssT1I CCWWGG 2 cut(s) 1311, 1363
Bst1107I GTATAC 1 cut(s) 1180
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 5 cut(s) 380, 506, 605, 1381, 2325
Bst6I CTCTTC 1 cut(s) 1196
BstAFI CTTAAG 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 1763
BstAUI TGTACA 4 cut(s) 59, 101, 1822, 1968
BstC8I GCNNGC 4 cut(s) 333, 868, 1018, 2038
BstDEI CTNAG 7 cut(s) 421, 724, 1104, 1160, 1738, 1851, 1878
BstF5I GGATG 3 cut(s) 126, 1408, 2254
BstFNI CGCG 2 cut(s) 109, 2040
BstH2I RGCGCY 1 cut(s) 2125
BstHHI GCGC 2 cut(s) 54, 2124
BstKTI GATC 7 cut(s) 208, 467, 472, 532, 952, 1849, 2142
BstMAI GTCTC 3 cut(s) 415, 1529, 1707
BstMBI GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
BstMCI CGRYCG 1 cut(s) 769
BstMWI GCNNNNNNNGC 5 cut(s) 16, 328, 1763, 1918, 2087
BstNI CCWGG 1 cut(s) 614
BstNSI RCATGY 1 cut(s) 1396
BstSCI CCNGG 1 cut(s) 612
BstSFI CTRYAG 5 cut(s) 525, 1329, 1650, 1894, 2003
BstSLI GKGCMC 1 cut(s) 452
BstUI CGCG 2 cut(s) 109, 2040
BstV1I GCAGC 2 cut(s) 296, 1153
BstV2I GAAGAC 2 cut(s) 1965, 2016
BstX2I RGATCY 2 cut(s) 529, 1846
BstXI CCANNNNNNTGG 1 cut(s) 1626
BstYI RGATCY 2 cut(s) 529, 1846
BstZ17I GTATAC 1 cut(s) 1180
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 5 cut(s) 331, 790, 1362, 2159, 2223
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 459
BtsCI GGATG 3 cut(s) 126, 1408, 2254
BtsI GCAGTG 1 cut(s) 2000
BtsIMutI CAGTG 2 cut(s) 553, 2000
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 4 cut(s) 333, 868, 1018, 2038
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 2 cut(s) 54, 2124
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 8 cut(s) 60, 102, 181, 364, 1377, 1823, 1855, 1969
CviAII CATG 9 cut(s) 22, 810, 936, 1013, 1170, 1393, 1598, 1626, 1826
CviQI GTAC 8 cut(s) 60, 102, 181, 364, 1377, 1823, 1855, 1969
DdeI CTNAG 7 cut(s) 421, 724, 1104, 1160, 1738, 1851, 1878
DpnI GATC 7 cut(s) 207, 466, 471, 531, 951, 1848, 2141
DpnII GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
DraI TTTAAA 1 cut(s) 2103
DriI GACNNNNNGTC 1 cut(s) 1720
EaeI YGGCCR 3 cut(s) 329, 1360, 2221
Eam1104I CTCTTC 1 cut(s) 1196
Eam1105I GACNNNNNGTC 1 cut(s) 1720
EarI CTCTTC 1 cut(s) 1196
Ecl136II GAGCTC 2 cut(s) 1667, 1921
Eco130I CCWWGG 2 cut(s) 1311, 1363
Eco24I GRGCYC 2 cut(s) 1669, 1923
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 2 cut(s) 1667, 1921
Eco57I CTGAAG 1 cut(s) 263
EcoICRI GAGCTC 2 cut(s) 1667, 1921
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 612
EcoT14I CCWWGG 2 cut(s) 1311, 1363
EcoT22I ATGCAT 1 cut(s) 815
EcoT38I GRGCYC 2 cut(s) 1669, 1923
ErhI CCWWGG 2 cut(s) 1311, 1363
Esp3I CGTCTC 1 cut(s) 1529
FaeI CATG 9 cut(s) 25, 813, 939, 1016, 1173, 1396, 1601, 1629, 1829
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 1533, 1565
FaqI GGGAC 1 cut(s) 1383
FatI CATG 9 cut(s) 21, 809, 935, 1012, 1169, 1392, 1597, 1625, 1825
FauNDI CATATG 1 cut(s) 56
FblI GTMKAC 3 cut(s) 235, 1179, 1893
Fnu4HI GCNGC 3 cut(s) 310, 336, 1167
FokI GGATG 3 cut(s) 113, 1415, 2261
FriOI GRGCYC 2 cut(s) 1669, 1923
Fsp4HI GCNGC 3 cut(s) 310, 336, 1167
FspBI CTAG 4 cut(s) 11, 1074, 1137, 2262
GlaI GCGC 2 cut(s) 53, 2123
GluI GCNGC 3 cut(s) 310, 336, 1167
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 2125
HaeIII GGCC 5 cut(s) 331, 790, 1362, 2159, 2223
HapII CCGG 2 cut(s) 863, 1929
HhaI GCGC 2 cut(s) 54, 2124
Hin1II CATG 9 cut(s) 25, 813, 939, 1016, 1173, 1396, 1601, 1629, 1829
Hin6I GCGC 2 cut(s) 52, 2122
HinP1I GCGC 2 cut(s) 52, 2122
HincII GTYRAC 2 cut(s) 1398, 2277
HindII GTYRAC 2 cut(s) 1398, 2277
HindIII AAGCTT 1 cut(s) 721
HinfI GANTC 5 cut(s) 650, 1293, 1633, 2012, 2235
HpaII CCGG 2 cut(s) 863, 1929
HphI GGTGA 1 cut(s) 1799
Hpy166II GTNNAC 9 cut(s) 236, 450, 619, 1180, 1398, 1640, 1725, 1894, 2277
Hpy188III TCNNGA 7 cut(s) 226, 905, 1288, 1782, 1850, 1924, 2030
Hpy8I GTNNAC 9 cut(s) 236, 450, 619, 1180, 1398, 1640, 1725, 1894, 2277
Hpy99I CGWCG 3 cut(s) 460, 592, 704
HpyAV CCTTC 4 cut(s) 223, 899, 2045, 2279
HpyCH4III ACNGT 5 cut(s) 380, 506, 605, 1381, 2325
HpyCH4IV ACGT 3 cut(s) 458, 1213, 2253
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 328, 1763, 1918, 2087
HpyF3I CTNAG 7 cut(s) 421, 724, 1104, 1160, 1738, 1851, 1878
HpySE526I ACGT 3 cut(s) 458, 1213, 2253
Hsp92II CATG 9 cut(s) 25, 813, 939, 1016, 1173, 1396, 1601, 1629, 1829
HspAI GCGC 2 cut(s) 52, 2122
Kzo9I GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
LguI GCTCTTC 1 cut(s) 1196
LmnI GCTCC 3 cut(s) 714, 1664, 2296
Lsp1109I GCAGC 2 cut(s) 296, 1153
LweI GCATC 4 cut(s) 74, 135, 973, 2023
MaeI CTAG 4 cut(s) 11, 1074, 1137, 2262
MaeII ACGT 3 cut(s) 458, 1213, 2253
MaeIII GTNAC 2 cut(s) 283, 1317
MalI GATC 7 cut(s) 207, 466, 471, 531, 951, 1848, 2141
MboI GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
MboII GAAGA 6 cut(s) 94, 1213, 1419, 1970, 2021, 2127
MflI RGATCY 2 cut(s) 529, 1846
MhlI GDGCHC 5 cut(s) 452, 1208, 1669, 1923, 2293
MlsI TGGCCA 2 cut(s) 1362, 2223
MluNI TGGCCA 2 cut(s) 1362, 2223
MlyI GAGTC 2 cut(s) 644, 2006
MmeI TCCRAC 2 cut(s) 919, 2133
Mox20I TGGCCA 2 cut(s) 1362, 2223
Mph1103I ATGCAT 1 cut(s) 815
MroXI GAANNNNTTC 2 cut(s) 221, 693
MscI TGGCCA 2 cut(s) 1362, 2223
MseI TTAA 8 cut(s) 375, 668, 719, 743, 954, 1281, 1422, 2102
MslI CAYNNNNRTG 4 cut(s) 109, 457, 808, 1624
Msp20I TGGCCA 2 cut(s) 1362, 2223
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 718
MspI CCGG 2 cut(s) 863, 1929
MspR9I CCNGG 1 cut(s) 614
Mva1269I GAATGC 3 cut(s) 197, 608, 923
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 2 cut(s) 109, 2040
MwoI GCNNNNNNNGC 5 cut(s) 16, 328, 1763, 1918, 2087
NdeI CATATG 1 cut(s) 56
NdeII GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
NlaIII CATG 9 cut(s) 25, 813, 939, 1016, 1173, 1396, 1601, 1629, 1829
NlaIV GGNNCC 2 cut(s) 946, 1848
NmeAIII GCCGAG 1 cut(s) 307
NsiI ATGCAT 1 cut(s) 815
NspI RCATGY 1 cut(s) 1396
PciSI GCTCTTC 1 cut(s) 1196
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 699
PctI GAATGC 3 cut(s) 197, 608, 923
PdmI GAANNNNTTC 2 cut(s) 221, 693
PfeI GAWTC 3 cut(s) 1293, 1633, 2235
PflFI GACNNNGTC 1 cut(s) 900
PkrI GCNGC 3 cut(s) 311, 337, 1168
PleI GAGTC 2 cut(s) 644, 2006
PpsI GAGTC 2 cut(s) 644, 2006
PshBI ATTAAT 2 cut(s) 743, 1281
PsiI TTATAA 1 cut(s) 1230
Psp124BI GAGCTC 2 cut(s) 1669, 1923
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 946, 1848
PspPI GGNCC 1 cut(s) 149
PsrI GAACNNNNNNTAC 1 cut(s) 2304
PstI CTGCAG 1 cut(s) 2007
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 2 cut(s) 529, 1846
PsyI GACNNNGTC 1 cut(s) 900
RsaI GTAC 8 cut(s) 61, 103, 182, 365, 1378, 1824, 1856, 1970
RsaNI GTAC 8 cut(s) 60, 102, 181, 364, 1377, 1823, 1855, 1969
RseI CAYNNNNRTG 4 cut(s) 109, 457, 808, 1624
SacI GAGCTC 2 cut(s) 1669, 1923
SapI GCTCTTC 1 cut(s) 1196
SaqAI TTAA 8 cut(s) 375, 668, 719, 743, 954, 1281, 1422, 2102
SatI GCNGC 3 cut(s) 310, 336, 1167
Sau3AI GATC 7 cut(s) 205, 464, 469, 529, 949, 1846, 2139
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 2 cut(s) 365, 1856
SchI GAGTC 2 cut(s) 644, 2006
ScrFI CCNGG 1 cut(s) 614
SduI GDGCHC 5 cut(s) 452, 1208, 1669, 1923, 2293
SfaNI GCATC 4 cut(s) 74, 135, 973, 2023
SfcI CTRYAG 5 cut(s) 525, 1329, 1650, 1894, 2003
SinI GGWCC 1 cut(s) 149
SmiMI CAYNNNNRTG 4 cut(s) 109, 457, 808, 1624
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SsiI CCGC 4 cut(s) 109, 114, 335, 769
SspI AATATT 1 cut(s) 976
SspMI CTAG 4 cut(s) 11, 1074, 1137, 2262
SstI GAGCTC 2 cut(s) 1669, 1923
StyD4I CCNGG 1 cut(s) 612
StyI CCWWGG 2 cut(s) 1311, 1363
TaaI ACNGT 5 cut(s) 380, 506, 605, 1381, 2325
TaiI ACGT 3 cut(s) 461, 1216, 2256
TaqI TCGA 9 cut(s) 455, 472, 702, 765, 825, 841, 1287, 2233, 2238
TatI WGTACW 6 cut(s) 59, 101, 363, 1822, 1854, 1968
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 3 cut(s) 1293, 1633, 2235
Tru1I TTAA 8 cut(s) 375, 668, 719, 743, 954, 1281, 1422, 2102
Tru9I TTAA 8 cut(s) 375, 668, 719, 743, 954, 1281, 1422, 2102
TscAI CASTG 2 cut(s) 553, 2007
TseI GCWGC 2 cut(s) 309, 1166
TspDTI ATGAA 7 cut(s) 19, 585, 930, 1453, 1575, 1586, 2064
TspGWI ACGGA 4 cut(s) 605, 672, 1415, 1553
TspRI CASTG 2 cut(s) 553, 2007
Tth111I GACNNNGTC 1 cut(s) 900
Vha464I CTTAAG 1 cut(s) 718
VneI GTGCAC 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 149
VspI ATTAAT 2 cut(s) 743, 1281
XapI RAATTY 3 cut(s) 1460, 1562, 2216
XceI RCATGY 1 cut(s) 1396
XmiI GTMKAC 3 cut(s) 235, 1179, 1893
XmnI GAANNNNTTC 2 cut(s) 221, 693
XspI CTAG 4 cut(s) 11, 1074, 1137, 2262
ZrmI AGTACT 2 cut(s) 365, 1856
Zsp2I ATGCAT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.