RLG00000001421

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000111
Physical Location & Seq
Forward (+)
15416380 .. 15418702
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001421

Sequence Viewer

Length: 1008 bp
ATGCATTTCAACAAAGCCATTGAGAATGAAGATTTGTTTTACCAAAGAAGAAAACTGTTATTCACTGATGAAAAAGGTGATTCACTTTACTCTTTCGACCTCGATGAGTTTCTTAATCATTATCATGATCATCCATTTCTGAATGGTCCTGGTAACTGTAACAATGTTAATGTTGGTGTCATTGGTGATGATTATGATGGTGATCTAGTTGTCACTAGACTCAAACTGCCCAGTTGTGTTGGGGTCGCTACATATCCCAGTGAGACCTTGGTGTCTTTCTGCTGTGGCCTGGTGTTATTTAGGTTGGGTTCCGAGTTATATGTGGCTAACCCAGCAATAAGGAAATATAAGAAACTGCCAGCTCCAACTGCAGAGTTGGGTCTTGGACCCTTCATCTGTTACCAATTTGGGTTCGGTTTTGATCACACAACTGATGATTTCAAGGTGATTAAATCGACGTCCTGGTATAATGGAGTGGATTTTATTGTCTATGCATTCAAAACTGGTTCTTGGCGACAAATTCAAAGGCGATTGCCCTACATAAATTTTAGTCCTCGTACTGGGATAATGCTTAATGGAAGACTTCATTGTATGATGTGGAGCCTGAAAGGTTACTCATGGGTGATTGTATCTCTCTCTTTAGCAGAAGAGGAGGTTCAGGAAATTCCACTGCCGCTTGATTTAGATGTGACAATCTTGGATGACATGGAATTGGGACTGTTCTTCAAAGACAGGCTCTGTATAATTTATCCAGTTAGCACTGATAGGGTAGCTGTCAAGGTCTATCCATTCCTGGATATCCTAAAGTTACGGGGGCTGGTTTCTACTTGGAGAGCCTTGTTTCACTGGATGATCATCGAGATTTTAATGAGCATATCCAGCATGAGAGGAATCTTAGCTGAATACTGGATGAGATACACTCTTGTTGTCTTCCAGATGCTGACTTTTGTGGTGCACGATCGTAGTTTATGGTTGGTTTCTCTTGGAGGAATGTTTCAGTCGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

336

Amino Acids

38.63

Weight (kDa)

5.91

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 89 - 230 1.7e-13 F-box associated beta propeller domain
FBA_1 PF07734 92 - 251 9.2e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06240
fragaria_vesca FvH4_1g04511 FvH4_1g04521 FvH4_1g05182 FvH4_1g05210 FvH4_1g05230 FvH4_1g05231 FvH4_1g05232 FvH4_1g05391 FvH4_1g05420 FvH4_2g41451 FvH4_3g15810 FvH4_3g15810 FvH4_3g18161 FvH4_3g19801 FvH4_3g39980 FvH4_3g39980 FvH4_3g39980 FvH4_3g39990 FvH4_3g40020 FvH4_3g40020 FvH4_3g40031 FvH4_3g40040 FvH4_4g32891 FvH4_5g33121 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g38210 FvH4_5g38211 FvH4_5g39231 FvH4_6g16940 FvH4_6g33181 FvH4_7g09411 FvH4_7g23271 FvH4_7g26741 FvH4_7g28012 FvH4_7g28013 FvH4_7g28031 FvH4_7g28041
malus_domestica MD03G1104200.v1.1 MD03G1139900.v1.1 MD03G1192800.v1.1 MD10G1202200.v1.1 MD11G1131100.v1.1 MD11G1202700.v1.1 MD11G1203000.v1.1
prunus_persica Prupe.2G163500_v2.0.a1 Prupe.4G140000_v2.0.a1 Prupe.4G140100_v2.0.a1 Prupe.4G222200_v2.0.a1 Prupe.4G236200_v2.0.a1 Prupe.4G236300_v2.0.a1 Prupe.4G236400_v2.0.a1 Prupe.4G237500_v2.0.a1 Prupe.6G125500_v2.0.a1
pyrus_communis pycom03g14510 pycom05g20120 pycom11g10840 pycom11g17520 pycom11g17590 pycom15g03310
rosa_chinensis RchiOBHm_Chr0c44g0503541 RchiOBHm_Chr2g0090931 RchiOBHm_Chr2g0090941 RchiOBHm_Chr2g0090951 RchiOBHm_Chr2g0090961 RchiOBHm_Chr2g0092791 RchiOBHm_Chr2g0099041 RchiOBHm_Chr2g0169971 RchiOBHm_Chr2g0170021 RchiOBHm_Chr4g0386131 RchiOBHm_Chr4g0386141 RchiOBHm_Chr4g0386161 RchiOBHm_Chr4g0386281 RchiOBHm_Chr4g0386291 RchiOBHm_Chr4g0440811 RchiOBHm_Chr5g0026601 RchiOBHm_Chr5g0026621 RchiOBHm_Chr5g0026871 RchiOBHm_Chr5g0072021 RchiOBHm_Chr5g0072031 RchiOBHm_Chr5g0072171 RchiOBHm_Chr5g0072181 RchiOBHm_Chr7g0231281 RchiOBHm_Chr7g0231301 RchiOBHm_Chr7g0240141
rosa_laevigata RLG00000000695 RLG00000001421 RLG00000004300 RLG00000006148 RLG00000006151 RLG00000016157 RLG00000016158 RLG00000016159 RLG00000016160 RLG00000016161 RLG00000020474 RLG00000021915 RLG00000029159 RLG00000032939 RLG00000032940 RLG00000032966 RLG00000036270 RLG00000036272 RLG00000036280 RLG00000036284
rosa_multiflora Rmu_co8434269.1_g000001 Rmu_sc0000792.1_g000017 Rmu_sc0000792.1_g000018 Rmu_sc0000792.1_g000019 Rmu_sc0001132.1_g000008 Rmu_sc0001152.1_g000004 Rmu_sc0001152.1_g000033 Rmu_sc0001583.1_g000004 Rmu_sc0003305.1_g000001 Rmu_sc0003333.1_g000005 Rmu_sc0003396.1_g000010 Rmu_sc0003396.1_g000011 Rmu_sc0003396.1_g000017 Rmu_sc0004571.1_g000004 Rmu_sc0004851.1_g000003 Rmu_sc0004851.1_g000004 Rmu_sc0005008.1_g000015 Rmu_sc0006854.1_g000016 Rmu_sc0006854.1_g000023 Rmu_sc0009422.1_g000009 Rmu_sc0010610.1_g000003 Rmu_sc0018952.1_g000008 Rmu_sc0037136.1_g000001 Rmu_ssc0000172.1_g000016 Rmu_ssc0000389.1_g000005
rosa_roxburghii Rroxscaffold_1G00009050 Rroxscaffold_1G00009090 Rroxscaffold_1G00009130 Rroxscaffold_1G00009230 Rroxscaffold_1G00044650 Rroxscaffold_1G00052600 Rroxscaffold_1G00052610 Rroxscaffold_1G00052860 Rroxscaffold_1G00052880 Rroxscaffold_2G00081700 Rroxscaffold_2G00148820 Rroxscaffold_2G00150560 Rroxscaffold_2G00150570 Rroxscaffold_2G00150590 Rroxscaffold_2G00150600 Rroxscaffold_2G00150630 Rroxscaffold_2G00150730 Rroxscaffold_2G00150740 Rroxscaffold_3G00229360 Rroxscaffold_3G00262430 Rroxscaffold_4G00313090 Rroxscaffold_5G00381610
rosa_rugosa Rorug01G0285600 Rorug02G0011200 Rorug02G0011300 Rorug02G0011400 Rorug02G0012500 Rorug02G0012600 Rorug02G0012700 Rorug02G0029300 Rorug02G0075800 Rorug02G0075900 Rorug02G0419600 Rorug02G0544800 Rorug04G0112200 Rorug05G0094100 Rorug05G0094100 Rorug05G0094200 Rorug05G0415900 Rorug05G0415900 Rorug05G0416000 Rorug05G0416100 Rorug05G0416200 Rorug05G0416300 Rorug05G0416700 Rorug05G0416800 Rorug07G0265700 Rorug07G0265800 Rorug07G0265900 Rorug07G0266000 Rorug07G0266100
rosa_samantha Rh2AG057300 Rh2AG057400 Rh2AG058700 Rh2AG478800 Rh2AG616800 Rh2DG056700 Rh2DG056800 Rh2DG056900 Rh2DG073500 Rh2DG129700 Rh2DG639800 Rh4AG380100 Rh4DG386700 Rh5AG187500 Rh5AG187700 Rh5AG189200 Rh5AG472100 Rh5AG472800 Rh5BG184000 Rh5BG184200 Rh5BG185800 Rh5BG186500 Rh5CG204000 Rh5CG205800 Rh5DG186300 Rh5DG186500 Rh5DG188100 Rh5DG503500 Rh5DG503700 Rh5DG504000 Rh5DG504100 Rh5DG504700 Rh5DG504900 Rh5DG505000 Rh7AG419500 Rh7AG419800 Rh7CG439300 Rh7DG413100
rosa_wichuraiana Rw0G002880 Rw1G026240 Rw2G005200 Rw2G005210 Rw2G005820 Rw2G009640 Rw5G016980 Rw5G017000 Rw5G017190 Rw5G043880 Rw7G011300 Rw7G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AatII GACGTC 1 cut(s) 461
AciI CCGC 1 cut(s) 674
AcsI RAATTY 3 cut(s) 519, 544, 663
AcyI GRCGYC 1 cut(s) 458
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 1 cut(s) 560
AgsI TTSAA 5 cut(s) 10, 442, 499, 524, 727
AjnI CCWGG 4 cut(s) 148, 288, 461, 792
AluBI AGCT 3 cut(s) 362, 773, 899
AluI AGCT 3 cut(s) 362, 773, 899
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 1 cut(s) 257
Alw44I GTGCAC 1 cut(s) 953
AlwNI CAGNNNCTG 3 cut(s) 738, 940, 1003
AoxI GGCC 1 cut(s) 286
ApaLI GTGCAC 1 cut(s) 953
ApoI RAATTY 3 cut(s) 519, 544, 663
AspS9I GGNCC 2 cut(s) 146, 386
AsuHPI GGTGA 5 cut(s) 89, 197, 212, 457, 634
AvaII GGWCC 2 cut(s) 146, 386
BaeGI GKGCMC 1 cut(s) 957
BbsI GAAGAC 2 cut(s) 586, 922
Bbv12I GWGCWC 1 cut(s) 957
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 4 cut(s) 150, 290, 463, 794
BclI TGATCA 3 cut(s) 127, 421, 852
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 2 cut(s) 206, 216
BfmI CTRYAG 2 cut(s) 369, 1004
BisI GCNGC 1 cut(s) 674
BlsI GCNGC 1 cut(s) 675
Bme1390I CCNGG 4 cut(s) 150, 290, 463, 794
Bme18I GGWCC 2 cut(s) 146, 386
BmgT120I GGNCC 2 cut(s) 146, 386
BmiI GGNNCC 3 cut(s) 310, 388, 602
BmrFI CCNGG 4 cut(s) 150, 290, 463, 794
BmrI ACTGGG 3 cut(s) 225, 252, 570
BmsI GCATC 1 cut(s) 927
BmuI ACTGGG 3 cut(s) 225, 252, 570
BpiI GAAGAC 2 cut(s) 586, 922
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BsaBI GATNNNNATC 2 cut(s) 201, 854
BsaHI GRCGYC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 7 cut(s) 231, 258, 508, 565, 752, 851, 911
Bse8I GATNNNNATC 2 cut(s) 201, 854
BseBI CCWGG 4 cut(s) 150, 290, 463, 794
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 4 cut(s) 130, 706, 855, 915
BseJI GATNNNNATC 2 cut(s) 201, 854
BseLI CCNNNNNNNGG 1 cut(s) 560
BseNI ACTGG 7 cut(s) 231, 258, 508, 565, 752, 851, 911
BseRI GAGGAG 1 cut(s) 665
BseSI GKGCMC 1 cut(s) 957
BseYI CCCAGC 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 961
BshFI GGCC 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 961
BsiHKAI GWGCWC 1 cut(s) 957
BslFI GGGAC 1 cut(s) 729
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 729
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 1 cut(s) 288
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 957
Bsp143I GATC 5 cut(s) 127, 202, 421, 852, 958
BspACI CCGC 1 cut(s) 674
BspANI GGCC 1 cut(s) 288
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 3 cut(s) 310, 388, 602
BspMAI CTGCAG 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 257
BsrI ACTGG 7 cut(s) 231, 258, 508, 565, 752, 851, 911
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 5 cut(s) 127, 202, 421, 852, 958
BssNI GRCGYC 1 cut(s) 458
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 4 cut(s) 150, 290, 463, 794
Bst4CI ACNGT 3 cut(s) 57, 158, 720
Bst6I CTCTTC 1 cut(s) 642
BstACI GRCGYC 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 895
BstF5I GGATG 4 cut(s) 130, 706, 855, 915
BstKTI GATC 5 cut(s) 130, 205, 424, 855, 961
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 5 cut(s) 127, 202, 421, 852, 958
BstMCI CGRYCG 1 cut(s) 961
BstMWI GCNNNNNNNGC 3 cut(s) 332, 368, 879
BstNI CCWGG 4 cut(s) 150, 290, 463, 794
BstSCI CCNGG 4 cut(s) 148, 288, 461, 792
BstSFI CTRYAG 2 cut(s) 369, 1004
BstSLI GKGCMC 1 cut(s) 957
BstV2I GAAGAC 2 cut(s) 586, 922
BsuRI GGCC 1 cut(s) 288
BtsCI GGATG 4 cut(s) 130, 706, 855, 915
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 5 cut(s) 63, 265, 668, 759, 844
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 3 cut(s) 738, 940, 1003
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 2 cut(s) 146, 386
Csp6I GTAC 1 cut(s) 558
CviAII CATG 4 cut(s) 125, 618, 706, 883
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 1 cut(s) 895
DpnI GATC 5 cut(s) 129, 204, 423, 854, 960
DpnII GATC 5 cut(s) 127, 202, 421, 852, 958
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 2 cut(s) 146, 386
EcoRII CCWGG 4 cut(s) 148, 288, 461, 792
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 2 cut(s) 6, 496
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 128, 621, 709, 886
FaqI GGGAC 1 cut(s) 729
FatI CATG 4 cut(s) 124, 617, 705, 882
FbaI TGATCA 3 cut(s) 127, 421, 852
Fnu4HI GCNGC 1 cut(s) 674
FokI GGATG 4 cut(s) 117, 713, 862, 922
Fsp4HI GCNGC 1 cut(s) 674
FspBI CTAG 2 cut(s) 206, 216
GluI GCNGC 1 cut(s) 674
GsaI CCCAGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 4 cut(s) 128, 621, 709, 886
HinfI GANTC 3 cut(s) 80, 219, 891
HphI GGTGA 5 cut(s) 89, 197, 212, 457, 634
Hpy166II GTNNAC 1 cut(s) 955
Hpy188I TCNGA 2 cut(s) 141, 313
Hpy188III TCNNGA 4 cut(s) 125, 659, 859, 934
Hpy8I GTNNAC 1 cut(s) 955
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 3 cut(s) 57, 158, 720
HpyCH4IV ACGT 1 cut(s) 458
HpyCH4V TGCA 4 cut(s) 4, 371, 494, 955
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 368, 879
HpyF3I CTNAG 1 cut(s) 895
HpySE526I ACGT 1 cut(s) 458
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 4 cut(s) 128, 621, 709, 886
Ksp22I TGATCA 3 cut(s) 127, 421, 852
Kzo9I GATC 5 cut(s) 127, 202, 421, 852, 958
LmnI GCTCC 2 cut(s) 367, 600
LweI GCATC 1 cut(s) 927
MaeI CTAG 2 cut(s) 206, 216
MaeII ACGT 1 cut(s) 458
MaeIII GTNAC 7 cut(s) 152, 158, 211, 398, 611, 688, 807
MalI GATC 5 cut(s) 129, 204, 423, 854, 960
MboI GATC 5 cut(s) 127, 202, 421, 852, 958
MboII GAAGA 6 cut(s) 41, 60, 591, 659, 715, 922
MhlI GDGCHC 1 cut(s) 957
MluCI AATT 6 cut(s) 404, 519, 544, 663, 710, 744
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 389
MnlI CCTC 6 cut(s) 110, 564, 643, 646, 881, 980
Mph1103I ATGCAT 2 cut(s) 6, 496
MseI TTAA 5 cut(s) 114, 168, 450, 573, 866
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 4 cut(s) 150, 290, 463, 794
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 4 cut(s) 150, 290, 463, 794
MwoI GCNNNNNNNGC 3 cut(s) 332, 368, 879
NdeII GATC 5 cut(s) 127, 202, 421, 852, 958
NlaIII CATG 4 cut(s) 128, 621, 709, 886
NlaIV GGNNCC 3 cut(s) 310, 388, 602
NmuCI GTSAC 2 cut(s) 211, 688
NsiI ATGCAT 2 cut(s) 6, 496
PagI TCATGA 1 cut(s) 124
PctI GAATGC 1 cut(s) 494
PfeI GAWTC 2 cut(s) 80, 891
PfoI TCCNGGA 1 cut(s) 792
PkrI GCNGC 1 cut(s) 675
Ple19I CGATCG 1 cut(s) 961
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 148, 288, 461, 792
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 4 cut(s) 148, 288, 461, 792
PspN4I GGNNCC 3 cut(s) 310, 388, 602
PspPI GGNCC 2 cut(s) 146, 386
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 3 cut(s) 738, 940, 1003
PvuI CGATCG 1 cut(s) 961
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 5 cut(s) 114, 168, 450, 573, 866
SatI GCNGC 1 cut(s) 674
Sau3AI GATC 5 cut(s) 127, 202, 421, 852, 958
Sau96I GGNCC 2 cut(s) 146, 386
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 4 cut(s) 150, 290, 463, 794
SduI GDGCHC 1 cut(s) 957
SfaNI GCATC 1 cut(s) 927
SfcI CTRYAG 2 cut(s) 369, 1004
SinI GGWCC 2 cut(s) 146, 386
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 6 cut(s) 404, 519, 544, 663, 710, 744
SsiI CCGC 1 cut(s) 674
SspMI CTAG 2 cut(s) 206, 216
StyD4I CCNGG 4 cut(s) 148, 288, 461, 792
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 3 cut(s) 57, 158, 720
TaiI ACGT 1 cut(s) 461
TaqI TCGA 4 cut(s) 96, 102, 455, 858
TasI AATT 6 cut(s) 404, 519, 544, 663, 710, 744
TauI GCSGC 1 cut(s) 676
TfiI GAWTC 2 cut(s) 80, 891
Tru1I TTAA 5 cut(s) 114, 168, 450, 573, 866
Tru9I TTAA 5 cut(s) 114, 168, 450, 573, 866
TscAI CASTG 5 cut(s) 70, 265, 675, 766, 851
TseFI GTSAC 2 cut(s) 211, 688
Tsp45I GTSAC 2 cut(s) 211, 688
TspDTI ATGAA 4 cut(s) 42, 84, 382, 575
TspRI CASTG 5 cut(s) 70, 265, 675, 766, 851
VneI GTGCAC 1 cut(s) 953
VpaK11BI GGWCC 2 cut(s) 146, 386
XapI RAATTY 3 cut(s) 519, 544, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 2 cut(s) 206, 216
ZraI GACGTC 1 cut(s) 459
Zsp2I ATGCAT 2 cut(s) 6, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.