Rw1G026240

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000111
Physical Location & Seq
Forward (+)
53298888 .. 53300147
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026240.1

Sequence Viewer

Length: 1038 bp
ATGGAAGACCATGGCGAACCTAACTTGTACCTTCCACCAGAGATTATGGATGGTATTCTTTTATTGTTACCAGCCAAGTCCTTATGCCGCTTCAAGTGTGTATCAAAACCATGGAAAGCCATGATGTCTGACACTGATTTTGTTAACAGGTACATCAACAGAGCCACTGAGAACAAGGGTGTATTCCTCCAAAGAAGAAGAGTGATATTCACCGACGCGGCCCAGCCAAGTGTTCGAGTTCCGTTTTTCCATTCCTGCTATGGCCTGATTCTCCTCGAGTTATCTGATTTGGAGTTATTTTTGGTTAATCCTGCAACAAGTGAATCCAAGCAACTAGCAAGACCACGACAGCATCCTTACTGTTCTCACAATTATAATTATCACCAATATGGGTTTGGTTTTGATTCCTCCACTTCTCAATACCAGTTGGTTCAGGGAGACATCTTTACAGGTGATGAGGATTTTTTTTTTTTGGGGTGGGAGGGAAACTGGTTCAGTGTCTATAACTTCGAAACTGATTCGTGGCTAGAGGAATTATCATCCCCGCATCCTTACATTCCTATAAGTTCACACTTACATCGAAACGAAAATGGGGTCATGTTGAATGGTCGTTTCCATTGGTTGATGGAATCATTCAGCCGATACGATGAAGCAATTGTGATTGTAGCTTTGGATGCACCGGTGTTAGAGACTCAAAAAATTCCACTACTGCTTGAATTATCCAGTGGTGGTCAATTGTTGAAACTAGGGGCCTTCAGAAAGTGGTTATGCATAACATTAGCGACACTCGAGCCCCATCTTGCACAAACATATAGCGAGTTTTGGGTCATGAAGGAATATGGAGTGAGGGAGTCGTGGACTAAAATGAGGGTCTCCATCCCTTATGCTGAGTTAGTTCTGTACAATTTTGATGAAGAAGATCCACAAACCTTCTGGAATTTATTAGTTCGCGAAATTGGCAAAGTTGGTAGAATTGGAGTATACATTGAGAGCCTTGTTTCAATTAGGAATCTCAACAGCATGAGAATAAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

345

Amino Acids

40.08

Weight (kDa)

5.51

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 47 3.6e-06 F-box domain
FBA_1 PF07734 71 - 297 1.9e-16 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06240
fragaria_vesca FvH4_1g04511 FvH4_1g04521 FvH4_1g05182 FvH4_1g05210 FvH4_1g05230 FvH4_1g05231 FvH4_1g05232 FvH4_1g05391 FvH4_1g05420 FvH4_2g41451 FvH4_3g15810 FvH4_3g15810 FvH4_3g18161 FvH4_3g19801 FvH4_3g39980 FvH4_3g39980 FvH4_3g39980 FvH4_3g39990 FvH4_3g40020 FvH4_3g40020 FvH4_3g40031 FvH4_3g40040 FvH4_4g32891 FvH4_5g33121 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g33140 FvH4_5g38210 FvH4_5g38211 FvH4_5g39231 FvH4_6g16940 FvH4_6g33181 FvH4_7g09411 FvH4_7g23271 FvH4_7g26741 FvH4_7g28012 FvH4_7g28013 FvH4_7g28031 FvH4_7g28041
malus_domestica MD03G1104200.v1.1 MD03G1139900.v1.1 MD03G1192800.v1.1 MD10G1202200.v1.1 MD11G1131100.v1.1 MD11G1202700.v1.1 MD11G1203000.v1.1
prunus_persica Prupe.2G163500_v2.0.a1 Prupe.4G140000_v2.0.a1 Prupe.4G140100_v2.0.a1 Prupe.4G222200_v2.0.a1 Prupe.4G236200_v2.0.a1 Prupe.4G236300_v2.0.a1 Prupe.4G236400_v2.0.a1 Prupe.4G237500_v2.0.a1 Prupe.6G125500_v2.0.a1
pyrus_communis pycom03g14510 pycom05g20120 pycom11g10840 pycom11g17520 pycom11g17590 pycom15g03310
rosa_chinensis RchiOBHm_Chr0c44g0503541 RchiOBHm_Chr2g0090931 RchiOBHm_Chr2g0090941 RchiOBHm_Chr2g0090951 RchiOBHm_Chr2g0090961 RchiOBHm_Chr2g0092791 RchiOBHm_Chr2g0099041 RchiOBHm_Chr2g0169971 RchiOBHm_Chr2g0170021 RchiOBHm_Chr4g0386131 RchiOBHm_Chr4g0386141 RchiOBHm_Chr4g0386161 RchiOBHm_Chr4g0386281 RchiOBHm_Chr4g0386291 RchiOBHm_Chr4g0440811 RchiOBHm_Chr5g0026601 RchiOBHm_Chr5g0026621 RchiOBHm_Chr5g0026871 RchiOBHm_Chr5g0072021 RchiOBHm_Chr5g0072031 RchiOBHm_Chr5g0072171 RchiOBHm_Chr5g0072181 RchiOBHm_Chr7g0231281 RchiOBHm_Chr7g0231301 RchiOBHm_Chr7g0240141
rosa_laevigata RLG00000000695 RLG00000001421 RLG00000004300 RLG00000006148 RLG00000006151 RLG00000016157 RLG00000016158 RLG00000016159 RLG00000016160 RLG00000016161 RLG00000020474 RLG00000021915 RLG00000029159 RLG00000032939 RLG00000032940 RLG00000032966 RLG00000036270 RLG00000036272 RLG00000036280 RLG00000036284
rosa_multiflora Rmu_co8434269.1_g000001 Rmu_sc0000792.1_g000017 Rmu_sc0000792.1_g000018 Rmu_sc0000792.1_g000019 Rmu_sc0001132.1_g000008 Rmu_sc0001152.1_g000004 Rmu_sc0001152.1_g000033 Rmu_sc0001583.1_g000004 Rmu_sc0003305.1_g000001 Rmu_sc0003333.1_g000005 Rmu_sc0003396.1_g000010 Rmu_sc0003396.1_g000011 Rmu_sc0003396.1_g000017 Rmu_sc0004571.1_g000004 Rmu_sc0004851.1_g000003 Rmu_sc0004851.1_g000004 Rmu_sc0005008.1_g000015 Rmu_sc0006854.1_g000016 Rmu_sc0006854.1_g000023 Rmu_sc0009422.1_g000009 Rmu_sc0010610.1_g000003 Rmu_sc0018952.1_g000008 Rmu_sc0037136.1_g000001 Rmu_ssc0000172.1_g000016 Rmu_ssc0000389.1_g000005
rosa_roxburghii Rroxscaffold_1G00009050 Rroxscaffold_1G00009090 Rroxscaffold_1G00009130 Rroxscaffold_1G00009230 Rroxscaffold_1G00044650 Rroxscaffold_1G00052600 Rroxscaffold_1G00052610 Rroxscaffold_1G00052860 Rroxscaffold_1G00052880 Rroxscaffold_2G00081700 Rroxscaffold_2G00148820 Rroxscaffold_2G00150560 Rroxscaffold_2G00150570 Rroxscaffold_2G00150590 Rroxscaffold_2G00150600 Rroxscaffold_2G00150630 Rroxscaffold_2G00150730 Rroxscaffold_2G00150740 Rroxscaffold_3G00229360 Rroxscaffold_3G00262430 Rroxscaffold_4G00313090 Rroxscaffold_5G00381610
rosa_rugosa Rorug01G0285600 Rorug02G0011200 Rorug02G0011300 Rorug02G0011400 Rorug02G0012500 Rorug02G0012600 Rorug02G0012700 Rorug02G0029300 Rorug02G0075800 Rorug02G0075900 Rorug02G0419600 Rorug02G0544800 Rorug04G0112200 Rorug05G0094100 Rorug05G0094100 Rorug05G0094200 Rorug05G0415900 Rorug05G0415900 Rorug05G0416000 Rorug05G0416100 Rorug05G0416200 Rorug05G0416300 Rorug05G0416700 Rorug05G0416800 Rorug07G0265700 Rorug07G0265800 Rorug07G0265900 Rorug07G0266000 Rorug07G0266100
rosa_samantha Rh2AG057300 Rh2AG057400 Rh2AG058700 Rh2AG478800 Rh2AG616800 Rh2DG056700 Rh2DG056800 Rh2DG056900 Rh2DG073500 Rh2DG129700 Rh2DG639800 Rh4AG380100 Rh4DG386700 Rh5AG187500 Rh5AG187700 Rh5AG189200 Rh5AG472100 Rh5AG472800 Rh5BG184000 Rh5BG184200 Rh5BG185800 Rh5BG186500 Rh5CG204000 Rh5CG205800 Rh5DG186300 Rh5DG186500 Rh5DG188100 Rh5DG503500 Rh5DG503700 Rh5DG504000 Rh5DG504100 Rh5DG504700 Rh5DG504900 Rh5DG505000 Rh7AG419500 Rh7AG419800 Rh7CG439300 Rh7DG413100
rosa_wichuraiana Rw0G002880 Rw1G026240 Rw2G005200 Rw2G005210 Rw2G005820 Rw2G009640 Rw5G016980 Rw5G017000 Rw5G017190 Rw5G043880 Rw7G011300 Rw7G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccI GTMKAC 1 cut(s) 981
AccII CGCG 2 cut(s) 218, 951
AciI CCGC 3 cut(s) 88, 218, 545
AclWI GGATC 1 cut(s) 914
AcsI RAATTY 2 cut(s) 699, 937
AcuI CTGAAG 1 cut(s) 739
AfaI GTAC 3 cut(s) 29, 152, 902
AgeI ACCGGT 1 cut(s) 679
AgsI TTSAA 5 cut(s) 94, 604, 716, 742, 1002
AluBI AGCT 1 cut(s) 668
AluI AGCT 1 cut(s) 668
Alw26I GTCTC 3 cut(s) 432, 683, 877
AlwI GGATC 1 cut(s) 914
Ama87I CYCGRG 2 cut(s) 275, 788
AoxI GGCC 3 cut(s) 219, 262, 750
ApoI RAATTY 2 cut(s) 699, 937
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 579, 611
AsiGI ACCGGT 1 cut(s) 679
AspS9I GGNCC 2 cut(s) 220, 750
AsuHPI GGTGA 3 cut(s) 202, 374, 464
AsuII TTCGAA 1 cut(s) 510
AvaI CYCGRG 2 cut(s) 275, 788
BanII GRGCYC 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 12
BccI CCATC 4 cut(s) 44, 619, 804, 884
BcoDI GTCTC 3 cut(s) 432, 683, 877
BfaI CTAG 3 cut(s) 335, 527, 746
BisI GCNGC 2 cut(s) 88, 219
BlsI GCNGC 2 cut(s) 89, 220
BmeT110I CYCGRG 2 cut(s) 275, 788
BmgT120I GGNCC 2 cut(s) 220, 750
BmiI GGNNCC 1 cut(s) 751
BmsI GCATC 3 cut(s) 361, 556, 664
BpiI GAAGAC 1 cut(s) 12
Bpu14I TTCGAA 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 877
BsaJI CCNNGG 2 cut(s) 10, 110
BsaWI WCCGGW 1 cut(s) 679
Bse118I RCCGGY 1 cut(s) 679
Bse1I ACTGG 3 cut(s) 424, 494, 723
BseDI CCNNGG 2 cut(s) 10, 110
BseGI GGATG 6 cut(s) 55, 352, 539, 547, 679, 876
BseMII CTCAG 2 cut(s) 159, 879
BseNI ACTGG 3 cut(s) 424, 494, 723
BseRI GAGGAG 1 cut(s) 263
BseYI CCCAGC 1 cut(s) 222
Bsh1236I CGCG 2 cut(s) 218, 951
BshFI GGCC 3 cut(s) 221, 264, 752
BshTI ACCGGT 1 cut(s) 679
BsiHKCI CYCGRG 2 cut(s) 275, 788
BsiSI CCGG 1 cut(s) 680
BsmAI GTCTC 3 cut(s) 432, 683, 877
BsnI GGCC 3 cut(s) 221, 264, 752
Bso31I GGTCTC 1 cut(s) 877
BsoBI CYCGRG 2 cut(s) 275, 788
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 795
Bsp1407I TGTACA 1 cut(s) 900
Bsp143I GATC 1 cut(s) 919
Bsp19I CCATGG 2 cut(s) 10, 110
Bsp68I TCGCGA 1 cut(s) 951
BspACI CCGC 3 cut(s) 88, 218, 545
BspANI GGCC 3 cut(s) 221, 264, 752
BspCNI CTCAG 2 cut(s) 160, 880
BspFNI CGCG 2 cut(s) 218, 951
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 1 cut(s) 751
BspPI GGATC 1 cut(s) 914
BspT104I TTCGAA 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 679
BsrGI TGTACA 1 cut(s) 900
BsrI ACTGG 3 cut(s) 424, 494, 723
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 2 cut(s) 10, 110
BssMI GATC 1 cut(s) 919
BssNAI GTATAC 1 cut(s) 982
BssT1I CCWWGG 2 cut(s) 10, 110
Bst1107I GTATAC 1 cut(s) 982
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 193
BstAUI TGTACA 1 cut(s) 900
BstBI TTCGAA 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 168, 888
BstDSI CCRYGG 2 cut(s) 10, 110
BstF5I GGATG 6 cut(s) 55, 352, 539, 547, 679, 876
BstFNI CGCG 2 cut(s) 218, 951
BstKTI GATC 1 cut(s) 922
BstMAI GTCTC 3 cut(s) 432, 683, 877
BstMBI GATC 1 cut(s) 919
BstMWI GCNNNNNNNGC 2 cut(s) 674, 957
BstUI CGCG 2 cut(s) 218, 951
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
BstZ17I GTATAC 1 cut(s) 982
BsuRI GGCC 3 cut(s) 221, 264, 752
BtgI CCRYGG 2 cut(s) 10, 110
BtsCI GGATG 6 cut(s) 55, 352, 539, 547, 679, 876
BtsIMutI CAGTG 4 cut(s) 132, 165, 502, 730
BtuMI TCGCGA 1 cut(s) 951
CciI TCATGA 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 2 cut(s) 220, 750
CseI GACGC 1 cut(s) 224
Csp6I GTAC 3 cut(s) 28, 151, 901
CspAI ACCGGT 1 cut(s) 679
CviAII CATG 6 cut(s) 11, 111, 121, 598, 829, 1021
CviQI GTAC 3 cut(s) 28, 151, 901
DdeI CTNAG 2 cut(s) 168, 888
DpnI GATC 1 cut(s) 921
DpnII GATC 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 10, 110
Eco24I GRGCYC 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 877
Eco57I CTGAAG 1 cut(s) 739
Eco88I CYCGRG 2 cut(s) 275, 788
EcoO109I RGGNCCY 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 10, 110
EcoT22I ATGCAT 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 2 cut(s) 10, 110
FaeI CATG 6 cut(s) 14, 114, 124, 601, 832, 1024
FatI CATG 6 cut(s) 10, 110, 120, 597, 828, 1020
FauI CCCGC 1 cut(s) 552
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 2 cut(s) 88, 219
FokI GGATG 6 cut(s) 62, 339, 526, 534, 686, 863
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 2 cut(s) 88, 219
FspBI CTAG 3 cut(s) 335, 527, 746
GluI GCNGC 2 cut(s) 88, 219
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 3 cut(s) 221, 264, 752
HapII CCGG 1 cut(s) 680
HgaI GACGC 1 cut(s) 224
Hin1II CATG 6 cut(s) 14, 114, 124, 601, 832, 1024
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 8 cut(s) 268, 323, 404, 518, 629, 691, 851, 1009
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 680
HphI GGTGA 3 cut(s) 202, 374, 464
Hpy166II GTNNAC 4 cut(s) 145, 569, 858, 982
Hpy188I TCNGA 3 cut(s) 130, 286, 758
Hpy188III TCNNGA 3 cut(s) 829, 934, 950
Hpy8I GTNNAC 4 cut(s) 145, 569, 858, 982
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 41, 763, 826, 940
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 4 cut(s) 314, 677, 771, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 674, 957
HpyF3I CTNAG 2 cut(s) 168, 888
Hsp92II CATG 6 cut(s) 14, 114, 124, 601, 832, 1024
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 1 cut(s) 919
LweI GCATC 3 cut(s) 361, 556, 664
MaeI CTAG 3 cut(s) 335, 527, 746
MaeIII GTNAC 1 cut(s) 66
MalI GATC 1 cut(s) 921
MboI GATC 1 cut(s) 919
MboII GAAGA 5 cut(s) 17, 207, 210, 926, 929
MfeI CAATTG 2 cut(s) 654, 734
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 1 cut(s) 795
MlyI GAGTC 2 cut(s) 685, 860
MnlI CCTC 8 cut(s) 197, 284, 418, 451, 475, 523, 840, 861
Mph1103I ATGCAT 1 cut(s) 773
MseI TTAA 2 cut(s) 144, 306
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 1 cut(s) 680
MunI CAATTG 2 cut(s) 654, 734
MvnI CGCG 2 cut(s) 218, 951
MwoI GCNNNNNNNGC 2 cut(s) 674, 957
NcoI CCATGG 2 cut(s) 10, 110
NdeII GATC 1 cut(s) 919
NlaIII CATG 6 cut(s) 14, 114, 124, 601, 832, 1024
NlaIV GGNNCC 1 cut(s) 751
NruI TCGCGA 1 cut(s) 951
NsiI ATGCAT 1 cut(s) 773
NspV TTCGAA 1 cut(s) 510
PaeR7I CTCGAG 2 cut(s) 275, 788
PagI TCATGA 1 cut(s) 828
PfeI GAWTC 6 cut(s) 268, 323, 404, 518, 629, 1009
PinAI ACCGGT 1 cut(s) 679
PkrI GCNGC 2 cut(s) 89, 220
PleI GAGTC 2 cut(s) 685, 859
PpsI GAGTC 2 cut(s) 685, 859
PsiI TTATAA 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 2 cut(s) 220, 750
PspXI VCTCGAGB 2 cut(s) 275, 788
PsuI RGATCY 1 cut(s) 919
RruI TCGCGA 1 cut(s) 951
RsaI GTAC 3 cut(s) 29, 152, 902
RsaNI GTAC 3 cut(s) 28, 151, 901
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 2 cut(s) 144, 306
SatI GCNGC 2 cut(s) 88, 219
Sau3AI GATC 1 cut(s) 919
Sau96I GGNCC 2 cut(s) 220, 750
SchI GAGTC 2 cut(s) 685, 860
SduI GDGCHC 1 cut(s) 795
SetI ASST 6 cut(s) 22, 33, 152, 454, 670, 932
SfaNI GCATC 3 cut(s) 361, 556, 664
Sfr274I CTCGAG 2 cut(s) 275, 788
SfuI TTCGAA 1 cut(s) 510
SgrAI CRCCGGYG 1 cut(s) 679
SlaI CTCGAG 2 cut(s) 275, 788
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 275, 788
SmoI CTYRAG 2 cut(s) 275, 788
SsiI CCGC 3 cut(s) 88, 218, 545
SspMI CTAG 3 cut(s) 335, 527, 746
StyI CCWWGG 2 cut(s) 10, 110
TaaI ACNGT 1 cut(s) 362
TaqI TCGA 5 cut(s) 235, 276, 510, 580, 789
TatI WGTACW 1 cut(s) 900
TauI GCSGC 2 cut(s) 90, 221
TfiI GAWTC 6 cut(s) 268, 323, 404, 518, 629, 1009
Tru1I TTAA 2 cut(s) 144, 306
Tru9I TTAA 2 cut(s) 144, 306
TscAI CASTG 4 cut(s) 139, 172, 502, 730
TspDTI ATGAA 3 cut(s) 663, 845, 927
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 4 cut(s) 139, 172, 502, 730
XapI RAATTY 2 cut(s) 699, 937
XcmI CCANNNNNNNNNTGG 3 cut(s) 257, 392, 930
XhoI CTCGAG 2 cut(s) 275, 788
XmiI GTMKAC 1 cut(s) 981
XspI CTAG 3 cut(s) 335, 527, 746
Zsp2I ATGCAT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.