Rorug06G0008900

ZnF_TTF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000122
Physical Location & Seq
Forward (+)
1368385 .. 1373245
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0008900.1

Sequence Viewer

Length: 3381 bp
ATGACTACCCAAACAGATACTCCTTCTTCTTCTGCTCCTCTCCCTCCGATCCCTAAATACGGTGTCTTTCTTAGTTCCAGCGGAGAAGACACCGATCAAAATTTTACAGATCATCTCTGTACTGCTCTGAACCAGGAGGGGATATTGACACTTAGAGATGGTGAAGAACTCGAGAGGGGAAAAGCAATTGAAGAATCAAGATGTGCAATTGTGGTTCTTTCAGAAAACTACAACCATCCATGGTGTTTGGATGCGATTGCAAAGATCGTTGAATGCAAGGAAGTGATGATGAAACTGGGAGTACTCCCAGTCTTTCTTGATGTGAATCCATCTGATGTACGAAAACAAACAGGGGATCATTTCGGGGAAGCCTTCTCGAAGCATGAAGAGGCCTTTAAACACGAACCGGAGAAGGTGCGCAGGTGGAGACGTGCTCTTACTTCAGTAGCCAATCTCTCTGGATGGGTTGTGCGAAATGGTCATGACGCCAAAGTTATTCAAGAAATGACAGAAGGTATTCTCAGAGATTTCTTTCCTAGAATCCCAAGTGTACCTGAGGGATTAGTTGGGATGGATTCTCACCTGAACGAAATGCTCTCGTACTTGGAATTGGGGTGGCCTGGAGTTCGTACCGTAGGGATTTGGGGGATGGGGGGTATCGGAAAGACAACTATTGCAAAAGTTGTTTATGAAAGGATTTATTCTCAGTTTGAAGGTCACGTCTTTCTTGAGAATGTCAGAGAATCAAGTCGTAAGCCAGGTGGCAGGGTTGGTTTACAAGAGGAACTTCTTTCAAAGTTGCTGAGACGGGATCATGTAAATGTATCTGACACATCTGAGGGAATAAGCATGATCAATCACAGACTACGTGCTAGAATGGTTCTTATCGTTCTTGATGATGTGGATGGACTAGACCAATTGGAAGCATTGTGTGGTGATGCTAGTTGGTTCGGGTCAGGGAGTAGAATCATCATAACATCAAGAGATGAGCATGTGCTGACAAATTTTGGAGTGGAGAAGATATACAAGGTCAATGCATTATCTGATGTTGAGGCTCTTCAGCTGTTTTGTTCAAGAGCCTTCAAGAAGGACCAGGTTGGAGAAGATTTTCTCAGGCTTTCTAAGGAGTTTGTAAAATATGCTGCTGGCCTTCCATTAGCTCTTCAAGTATTGGCTGCATCTCTCTGTGGTAAGGAAAAAACTCTGTGGTCAAGTGCATTGGATAGACTAAAACAGAAACCTGATAGACAAATTATGAATGTGCTTAAAGTAAGTTACGATGAACTAGATAAAATGGAAAAGGAAATTTTTTTGCACATTGCATGTTTCTTTAAAGGTGAGAACGTAGATCGTGTAGAAAGAATACTAAGGGTTGATTATTATCCAAACTTTGGTATCAGAGTTCTTCTGGATAGATCTCTAGTAACTGTGGTTGGAAGAAAATTATGGATGCATGACCTGCTGCAACAATTGGGTAAAGAAATTGTTTTTCAAGAATGTCTTGAAGAGCCTGGCAATCGTAGCAGGTTGTGGGTTCCGCAAGAAATCATCCATGTACTCGAGAAGAATAAGGGTTCAACTGCACTTCAAGCCATAGCCTTAGACTTTCCAAAAACAGAAGAGATACACTGCCACTCAGAGGCATTCTCTGTGATGCCCAGACTTGAGTTTCTCCAAATCCGTAATGTGCATATGAAGGAAGGCCCCAGTTATCTTCCTGGGGGATTAGTAGTTCTGGAATGGAGTGGGTATCCTATAGAAAGTCTCCCACAAAATTTCAATCCTAAGCGATTATGTGAACTTAACCTCTGCTACAGCAGCATTGAACAACTTTGGAGTGGAATAAAGAAATTGCCCTATTTGACAGTTATCAATTTAAGCTACTCTTGGAAGTTAATCAGGACCCCAGATTTTACACATGCTCCAGGGCTTGAAAGATTAATTCTTGAAGGTTGTACAAGTTTAGTGGACATTCACCCATCCATTGCTCACCTGGAGAGGCTTATTAGCTTGAATCTTAAAGATTGCAGAAGTCTTGCAAGTCTTCCAAGTAAGCTTGAAATGAAATGCCTTCAAATTCTTGTTCTTTCAGGCTGTTCAAAAGTCAAGAAAATTCCAGATTTTGTGGGATCTATGGAAGGCTTGTTAGAACTTTATTTGGATGGGACTGCTATAGAGCAAATACCTCTATCAGTTCGACTTCTGACTGGCCTCATTTTATTAAATTTGAGAGATTGCAAAAATCTCAAGAGACTTCCAGGAGACATTGGCAATTTAAAGTCCCTCAAGCGTCTCAATATTTGTGGATGCTCAAAACTTGAAACACTACCGGACAGCTTGGGAAAGATAGACTGCTTGAAAGAGCTTGATGCGACTGGGGGTAGTTCCATAAGCAATATACCATCGTCTATTTCCCGTTTGGAAAGTCTTGAAGTGTTATCTCTTTGTGGATGTAAAGGGATGTCAGGCAACATGACAAGATATCCACTCAGTTTTCTGTTGCACAAAAGTGATTCAGGTGGCTTGCGTTCTTTAACTGAGCTAAATCTCAGTGACTGTAATCTTCAGGAGGGAAGCATACCGGACGATTTTGGCTGCTTATTCCCTTTAGTTTCACTAAATCTAAGCAAAAACAACTTTGTTAGTCTTCCAAAAAGCATCAGACAGCTTTCAAAGCTTCGAAACTTGAATTTGGAAAGTTGTAAGACTCTTCAAAAATTGCCAGACCTTTCATCAAGCATAAACTTCAGTTTTGGGGCAGAGGGCAGTATTTCACAGGAAAGGCTGTCATCATGTTTCAATTTGATTAATTGCTCCAAATTGGTTGTCAATCAACGATGCAACAACATAGCATTGAAAATGCTATCGAGATTTCTTCAGGGAATTCCTTCTGCAGGAAATAGATTTGAAACTATAATTCCTGGCAGTGAAATCTCTGAGCTGTTCAGTGATCAAAGTGTGGAACCAGTTATAAGTATGGAGCTACCTCGACAATGGCACAAGAATAAGTGGATGGGATATGCATTTTGTGCTTGTTTTGTTATCCGTCGAGATCTTCCAGTTCCAGCTAACCGGCTTGGTAAATGGAAGTTCGGGACCCATAGCACTGCAAATGGTCTTCGATGCGAAGTGAAGCCTGAGAATCTGGGTGTGGCTGGCTGGTGTCCTTCCTTTGCCTGCAGCCAAGAATTAGGCCAAATTGAGTCGGAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTGTCATAACATTGAGTTTACATTCAAAACATTAGGCACTGGAATGGAGGTGAAGAAGTGTGGAGTCCGTCTGATATACGAGCAAGATTTAGATTTGACTGGAATGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4371 Dimer_Tnp_hAT

Protein Analysis

1126

Amino Acids

126.27

Weight (kDa)

6.61

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 173 4.2e-35 TIR domain
TIR_2 PF13676 22 - 111 6.8e-08 TIR domain
NB-ARC PF00931 208 - 364 1.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 432 - 497 3.8e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 680 - 817 5.8e-12 Leucine-rich repeat region
LRR_13 PF23286 690 - 774 5.3e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 774 - 910 5.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 973 - 1119 2e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19260 AT1G41920 AT1G42710 AT2G06541 AT2G06541 AT3G29450 AT3G29763 AT3G29765 AT3G31402 AT3G31402 AT4G09660
fragaria_vesca FvH4_1g22711 FvH4_3g10612 FvH4_3g23261 FvH4_3g31623 FvH4_3g33391 FvH4_3g37321 FvH4_3g43192 FvH4_3g43193 FvH4_4g00181 FvH4_4g00251 FvH4_4g08751 FvH4_4g15271 FvH4_5g27190 FvH4_6g02122 FvH4_6g26880 FvH4_6g35121 FvH4_7g16871 FvH4_7g19080 FvH4_7g30071
malus_domestica MD02G1162100.v1.1 MD03G1081700.v1.1 MD09G1280500.v1.1 MD12G1083800.v1.1 MD12G1128200.v1.1
prunus_persica Prupe.7G129800_v2.0.a1
pyrus_communis pycom01g08000 pycom02g13840 pycom02g18030 pycom04g01290 pycom04g12580 pycom05g02550 pycom05g06740 pycom05g26200 pycom07g09850 pycom08g10610 pycom10g11280 pycom11g12580 pycom11g22260 pycom12g18440 pycom13g20080 pycom15g25600 pycom16g23600 pycom17g10070
rosa_chinensis RchiOBHm_Chr1g0313551 RchiOBHm_Chr1g0364331 RchiOBHm_Chr1g0369741 RchiOBHm_Chr1g0380371 RchiOBHm_Chr2g0097391 RchiOBHm_Chr2g0113561 RchiOBHm_Chr2g0114151 RchiOBHm_Chr2g0119761 RchiOBHm_Chr2g0149851 RchiOBHm_Chr2g0151931 RchiOBHm_Chr2g0166411 RchiOBHm_Chr3g0453661 RchiOBHm_Chr3g0466101 RchiOBHm_Chr3g0477911 RchiOBHm_Chr3g0486441 RchiOBHm_Chr4g0390161 RchiOBHm_Chr4g0392191 RchiOBHm_Chr4g0414461 RchiOBHm_Chr4g0421421 RchiOBHm_Chr5g0023801 RchiOBHm_Chr5g0040121 RchiOBHm_Chr5g0045381 RchiOBHm_Chr5g0061511 RchiOBHm_Chr5g0062621 RchiOBHm_Chr5g0064161 RchiOBHm_Chr5g0068711 RchiOBHm_Chr6g0246041 RchiOBHm_Chr6g0252511 RchiOBHm_Chr6g0252521 RchiOBHm_Chr6g0263361 RchiOBHm_Chr6g0269451 RchiOBHm_Chr6g0275351 RchiOBHm_Chr6g0288481 RchiOBHm_Chr6g0310011 RchiOBHm_Chr6g0310021 RchiOBHm_Chr7g0223071 RchiOBHm_Chr7g0227621 RchiOBHm_Chr7g0242581
rosa_laevigata RLG00000033957
rosa_multiflora Rmu_co8455865.1_g000001 Rmu_sc0000021.1_g000011 Rmu_sc0000079.1_g000049 Rmu_sc0000087.1_g000015 Rmu_sc0000151.1_g000008 Rmu_sc0000365.1_g000014 Rmu_sc0000446.1_g000054 Rmu_sc0000446.1_g000055 Rmu_sc0000534.1_g000006 Rmu_sc0000724.1_g000020 Rmu_sc0000859.1_g000001 Rmu_sc0000876.1_g000002 Rmu_sc0000881.1_g000005 Rmu_sc0000929.1_g000014 Rmu_sc0001112.1_g000004 Rmu_sc0001136.1_g000009 Rmu_sc0001353.1_g000064 Rmu_sc0001662.1_g000005 Rmu_sc0001764.1_g000006 Rmu_sc0001982.1_g000015 Rmu_sc0002052.1_g000008 Rmu_sc0002052.1_g000009 Rmu_sc0002158.1_g000011 Rmu_sc0002285.1_g000005 Rmu_sc0002983.1_g000025 Rmu_sc0003063.1_g000006 Rmu_sc0003076.1_g000005 Rmu_sc0003221.1_g000053 Rmu_sc0003382.1_g000010 Rmu_sc0003733.1_g000054 Rmu_sc0003997.1_g000005 Rmu_sc0004283.1_g000008 Rmu_sc0004283.1_g000053 Rmu_sc0004509.1_g000007 Rmu_sc0004540.1_g000028 Rmu_sc0004657.1_g000005 Rmu_sc0004935.1_g000006 Rmu_sc0005072.1_g000003 Rmu_sc0005248.1_g000001 Rmu_sc0006161.1_g000001 Rmu_sc0006209.1_g000005 Rmu_sc0006576.1_g000018 Rmu_sc0006576.1_g000019 Rmu_sc0007121.1_g000016 Rmu_sc0007121.1_g000032 Rmu_sc0007753.1_g000016 Rmu_sc0007795.1_g000009 Rmu_sc0007841.1_g000019 Rmu_sc0008339.1_g000021 Rmu_sc0008339.1_g000022 Rmu_sc0008432.1_g000001 Rmu_sc0008633.1_g000009 Rmu_sc0009104.1_g000013 Rmu_sc0011469.1_g000001 Rmu_sc0012039.1_g000001 Rmu_sc0013947.1_g000007 Rmu_sc0015560.1_g000001 Rmu_sc0017369.1_g000001 Rmu_sc0021648.1_g000001 Rmu_sc0022867.1_g000003 Rmu_sc0027801.1_g000001 Rmu_sc0027801.1_g000002 Rmu_sc0029861.1_g000001 Rmu_sc0030314.1_g000001 Rmu_sc0040149.1_g000001 Rmu_ssc0000089.1_g000028 Rmu_ssc0000480.1_g000033
rosa_roxburghii Rroxscaffold_1G00002540 Rroxscaffold_1G00013960 Rroxscaffold_1G00041160 Rroxscaffold_1G00045580 Rroxscaffold_3G00247310 Rroxscaffold_3G00267070 Rroxscaffold_4G00313830 Rroxscaffold_5G00372270 Rroxscaffold_6G00404800 Rroxscaffold_6G00406090
rosa_rugosa Rorug03G0269800 Rorug04G0142900 Rorug04G0152600 Rorug05G0223300.1 Rorug05G0266400 Rorug06G0008900 Rorug07G0086000 Rorug07G0205800 Rorug07G0270900
rosa_wichuraiana Rw0G001600 Rw0G017220 Rw0G017620 Rw0G018380 Rw1G002320 Rw1G020940 Rw1G021200 Rw1G022610 Rw1G038680 Rw2G013320 Rw2G016850 Rw2G024020 Rw2G025210 Rw2G032270 Rw2G039490 Rw2G041630 Rw2G048450 Rw2G051510 Rw3G025320 Rw4G003260 Rw4G007300 Rw4G009290 Rw4G010910 Rw4G013260 Rw5G008190 Rw5G008290 Rw5G018740 Rw5G023640 Rw5G028800 Rw5G031830 Rw5G041280 Rw5G044050 Rw6G007060 Rw6G008230 Rw6G009960 Rw6G013460 Rw6G014610 Rw6G017780 Rw6G033200 Rw7G021340 Rw7G023750 Rw7G032290 Rw7G032450 Rw7G033880 Rw7G038580 Rw7G040180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2972
AarI CACCTGC 1 cut(s) 411
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 3 cut(s) 411, 1467, 1515
AccB7I CCANNNNNTGG 1 cut(s) 1391
AciI CCGC 2 cut(s) 81, 1538
AclWI GGATC 4 cut(s) 43, 363, 819, 2137
AcsI RAATTY 9 cut(s) 100, 1003, 1305, 1774, 2076, 2112, 2224, 2689, 2883
AcuI CTGAAG 5 cut(s) 426, 1043, 2549, 2731, 2861
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 8 cut(s) 121, 303, 339, 552, 602, 631, 1557, 1957
AfiI CCNNNNNNNGG 4 cut(s) 59, 1391, 1639, 2894
AjiI CACGTC 2 cut(s) 431, 721
AleI CACNNNNGTG 1 cut(s) 2508
AloI GAACNNNNNNTCC 2 cut(s) 1716, 1748
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 7 cut(s) 421, 799, 1769, 2245, 2256, 2297, 3235
AlwI GGATC 4 cut(s) 43, 363, 819, 2137
AlwNI CAGNNNCTG 1 cut(s) 2556
Ama87I CYCGRG 2 cut(s) 170, 1559
AoxI GGCC 6 cut(s) 390, 617, 1147, 1702, 2209, 3193
ApeKI GCWGC 6 cut(s) 1142, 1175, 1462, 1818, 2595, 3180
ApoI RAATTY 9 cut(s) 100, 1003, 1305, 1774, 2076, 2112, 2224, 2689, 2883
ArsI GACNNNNNNTTYG 2 cut(s) 2811, 2843
AseI ATTAAT 2 cut(s) 1940, 2808
Asp700I GAANNNNTTC 3 cut(s) 516, 1107, 2887
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 4 cut(s) 1090, 1703, 1902, 3096
AsuHPI GGTGA 8 cut(s) 173, 572, 947, 1349, 1967, 1982, 3248, 3328
AsuII TTCGAA 1 cut(s) 2680
AvaI CYCGRG 2 cut(s) 170, 1559
AvaII GGWCC 3 cut(s) 1090, 1902, 3096
AxyI CCTNAGG 1 cut(s) 555
BaeI ACNNNNGTAYC 2 cut(s) 1378, 1411
BbsI GAAGAC 4 cut(s) 93, 2036, 2639, 3110
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 6 cut(s) 1129, 1162, 1449, 1830, 2582, 3192
BciVI GTATCC 1 cut(s) 1761
BclI TGATCA 2 cut(s) 852, 2950
BcoDI GTCTC 7 cut(s) 421, 799, 1769, 2245, 2256, 2297, 3235
BfaI CTAG 6 cut(s) 537, 873, 911, 942, 1286, 1421
BfmI CTRYAG 5 cut(s) 1755, 1813, 2172, 2892, 3178
BfuAI ACCTGC 3 cut(s) 411, 1467, 1515
BfuI GTATCC 1 cut(s) 1761
BglII AGATCT 2 cut(s) 1415, 3052
BisI GCNGC 6 cut(s) 1143, 1176, 1463, 1819, 2596, 3181
BlsI GCNGC 6 cut(s) 1144, 1177, 1464, 1820, 2597, 3182
BmcAI AGTACT 1 cut(s) 303
Bme18I GGWCC 3 cut(s) 1090, 1902, 3096
BmeT110I CYCGRG 2 cut(s) 170, 1559
BmgBI CACGTC 2 cut(s) 431, 721
BmgT120I GGNCC 4 cut(s) 1090, 1703, 1902, 3096
BmiI GGNNCC 6 cut(s) 1536, 1705, 1904, 2964, 3097, 3098
BmrI ACTGGG 4 cut(s) 302, 305, 1701, 2385
BmuI ACTGGG 4 cut(s) 302, 305, 1701, 2385
BpiI GAAGAC 4 cut(s) 93, 2036, 2639, 3110
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 1631, 1663
BpmI CTGGAG 3 cut(s) 642, 1908, 2015
Bpu10I CCTNAGC 1 cut(s) 1785
Bpu14I TTCGAA 1 cut(s) 2680
BpuEI CTTGAG 4 cut(s) 749, 1685, 2231, 2270
BsaAI YACGTR 1 cut(s) 869
BsaBI GATNNNNATC 2 cut(s) 324, 1380
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 239, 1718, 1925
BsaWI WCCGGW 3 cut(s) 406, 2329, 2581
BsaXI ACNNNNNCTCC 3 cut(s) 34, 1906, 1936
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 1391, 1639, 2894
Bse118I RCCGGY 1 cut(s) 3072
Bse1I ACTGG 9 cut(s) 300, 308, 1707, 2212, 2380, 2966, 3059, 3310, 3370
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 2 cut(s) 1317, 1983
Bse8I GATNNNNATC 2 cut(s) 324, 1380
BseDI CCNNGG 3 cut(s) 239, 1718, 1925
BseJI GATNNNNATC 2 cut(s) 324, 1380
BseLI CCNNNNNNNGG 4 cut(s) 59, 1391, 1639, 2894
BseMI GCAATG 2 cut(s) 1317, 1983
BseNI ACTGG 9 cut(s) 300, 308, 1707, 2212, 2380, 2966, 3059, 3310, 3370
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 1129, 1162, 1449, 1830, 2582, 3192
BsgI GTGCAG 1 cut(s) 1566
BshFI GGCC 6 cut(s) 392, 619, 1149, 1704, 2211, 3195
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 2 cut(s) 170, 1559
BsiSI CCGG 4 cut(s) 407, 2330, 2582, 3073
BslFI GGGAC 3 cut(s) 2179, 2266, 3109
BslI CCNNNNNNNGG 4 cut(s) 59, 1391, 1639, 2894
BsmAI GTCTC 7 cut(s) 421, 799, 1769, 2245, 2256, 2297, 3235
BsmBI CGTCTC 3 cut(s) 421, 799, 2297
BsmFI GGGAC 3 cut(s) 2179, 2266, 3109
BsmI GAATGC 2 cut(s) 278, 1643
BsnI GGCC 6 cut(s) 392, 619, 1149, 1704, 2211, 3195
BsoBI CYCGRG 2 cut(s) 170, 1559
Bsp119I TTCGAA 1 cut(s) 2680
Bsp1286I GDGCHC 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 1955
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 2 cut(s) 81, 1538
BspANI GGCC 6 cut(s) 392, 619, 1149, 1704, 2211, 3195
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 6 cut(s) 1536, 1705, 1904, 2964, 3097, 3098
BspMAI CTGCAG 2 cut(s) 2896, 3182
BspMI ACCTGC 3 cut(s) 411, 1467, 1515
BspPI GGATC 4 cut(s) 43, 363, 819, 2137
BspQI GCTCTTC 3 cut(s) 1062, 1167, 1500
BspT104I TTCGAA 1 cut(s) 2680
BsrDI GCAATG 2 cut(s) 1317, 1983
BsrFI RCCGGY 1 cut(s) 3072
BsrGI TGTACA 1 cut(s) 1955
BsrI ACTGG 9 cut(s) 300, 308, 1707, 2212, 2380, 2966, 3059, 3310, 3370
BssAI RCCGGY 1 cut(s) 3072
BssECI CCNNGG 3 cut(s) 239, 1718, 1925
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 239
Bst4CI ACNGT 5 cut(s) 62, 634, 1429, 1867, 2558
Bst6I CTCTTC 6 cut(s) 381, 1062, 1167, 1500, 1614, 2715
BstACI GRCGYC 1 cut(s) 486
BstAPI GCANNNNNTGC 2 cut(s) 1459, 3029
BstAUI TGTACA 1 cut(s) 1955
BstBAI YACGTR 1 cut(s) 869
BstBI TTCGAA 1 cut(s) 2680
BstC8I GCNNGC 4 cut(s) 1147, 2525, 3157, 3178
BstDSI CCRYGG 1 cut(s) 239
BstENI CCTNNNNNAGG 1 cut(s) 2892
BstHHI GCGC 1 cut(s) 420
BstMAI GTCTC 7 cut(s) 421, 799, 1769, 2245, 2256, 2297, 3235
BstMWI GCNNNNNNNGC 6 cut(s) 1459, 1521, 1589, 1818, 2674, 3029
BstNSI RCATGY 3 cut(s) 995, 1326, 1922
BstSFI CTRYAG 5 cut(s) 1755, 1813, 2172, 2892, 3178
BstV1I GCAGC 6 cut(s) 1129, 1162, 1449, 1830, 2582, 3192
BstV2I GAAGAC 4 cut(s) 93, 2036, 2639, 3110
BstX2I RGATCY 3 cut(s) 1415, 2129, 3052
BstYI RGATCY 3 cut(s) 1415, 2129, 3052
Bsu36I CCTNAGG 1 cut(s) 555
BsuI GTATCC 1 cut(s) 1761
BsuRI GGCC 6 cut(s) 392, 619, 1149, 1704, 2211, 3195
BtgI CCRYGG 1 cut(s) 239
BtrI CACGTC 2 cut(s) 431, 721
BtsI GCAGTG 3 cut(s) 1627, 2932, 3105
BtsIMutI CAGTG 6 cut(s) 1627, 2557, 2932, 2953, 3105, 3303
BveI ACCTGC 3 cut(s) 411, 1467, 1515
Cac8I GCNNGC 4 cut(s) 1147, 2525, 3157, 3178
CaiI CAGNNNCTG 1 cut(s) 2556
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 3072
Cfr13I GGNCC 4 cut(s) 1090, 1703, 1902, 3096
CseI GACGC 2 cut(s) 494, 2279
CsiI ACCWGGT 1 cut(s) 1092
Csp6I GTAC 8 cut(s) 120, 302, 338, 551, 601, 630, 1556, 1956
CspCI CAANNNNNGTGG 4 cut(s) 1947, 1982, 2284, 2319
CviQI GTAC 8 cut(s) 120, 302, 338, 551, 601, 630, 1556, 1956
DraI TTTAAA 3 cut(s) 397, 1333, 2277
Eam1104I CTCTTC 6 cut(s) 381, 1062, 1167, 1500, 1614, 2715
EarI CTCTTC 6 cut(s) 381, 1062, 1167, 1500, 1614, 2715
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 392
Eco32I GATATC 1 cut(s) 2483
Eco47I GGWCC 3 cut(s) 1090, 1902, 3096
Eco57I CTGAAG 5 cut(s) 426, 1043, 2549, 2731, 2861
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 2 cut(s) 170, 1559
EcoNI CCTNNNNNAGG 1 cut(s) 2892
EcoO109I RGGNCCY 3 cut(s) 1703, 1902, 3096
EcoRI GAATTC 1 cut(s) 2883
EcoRV GATATC 1 cut(s) 2483
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 3 cut(s) 1039, 1455, 3025
ErhI CCWWGG 1 cut(s) 239
Esp3I CGTCTC 3 cut(s) 421, 799, 2297
FalI AAGNNNNNCTT 6 cut(s) 771, 803, 1485, 1517, 1870, 1902
FaqI GGGAC 3 cut(s) 2179, 2266, 3109
FauNDI CATATG 1 cut(s) 1692
FbaI TGATCA 2 cut(s) 852, 2950
Fnu4HI GCNGC 6 cut(s) 1143, 1176, 1463, 1819, 2596, 3181
Fsp4HI GCNGC 6 cut(s) 1143, 1176, 1463, 1819, 2596, 3181
FspBI CTAG 6 cut(s) 537, 873, 911, 942, 1286, 1421
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 1 cut(s) 419
GluI GCNGC 6 cut(s) 1143, 1176, 1463, 1819, 2596, 3181
GsuI CTGGAG 3 cut(s) 642, 1908, 2015
HaeIII GGCC 6 cut(s) 392, 619, 1149, 1704, 2211, 3195
HapII CCGG 4 cut(s) 407, 2330, 2582, 3073
HgaI GACGC 2 cut(s) 494, 2279
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 1 cut(s) 486
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 2 cut(s) 2054, 2675
HpaII CCGG 4 cut(s) 407, 2330, 2582, 3073
HphI GGTGA 8 cut(s) 173, 572, 947, 1349, 1967, 1982, 3248, 3328
Hpy166II GTNNAC 5 cut(s) 551, 776, 1799, 1969, 3285
Hpy8I GTNNAC 5 cut(s) 551, 776, 1799, 1969, 3285
Hpy99I CGWCG 1 cut(s) 3051
HpyCH4III ACNGT 5 cut(s) 62, 634, 1429, 1867, 2558
HpyCH4IV ACGT 4 cut(s) 430, 720, 868, 1344
HpyF10VI GCNNNNNNNGC 6 cut(s) 1459, 1521, 1589, 1818, 2674, 3029
HpySE526I ACGT 4 cut(s) 430, 720, 868, 1344
Hsp92I GRCGYC 1 cut(s) 486
HspAI GCGC 1 cut(s) 418
KflI GGGWCCC 1 cut(s) 3096
Ksp22I TGATCA 2 cut(s) 852, 2950
LguI GCTCTTC 3 cut(s) 1062, 1167, 1500
LmnI GCTCC 4 cut(s) 40, 1927, 2819, 2980
Lsp1109I GCAGC 6 cut(s) 1129, 1162, 1449, 1830, 2582, 3192
MabI ACCWGGT 1 cut(s) 1092
MaeI CTAG 6 cut(s) 537, 873, 911, 942, 1286, 1421
MaeII ACGT 4 cut(s) 430, 720, 868, 1344
MaeIII GTNAC 4 cut(s) 716, 1274, 1423, 2552
MfeI CAATTG 4 cut(s) 186, 207, 917, 1469
MflI RGATCY 3 cut(s) 1415, 2129, 3052
MhlI GDGCHC 1 cut(s) 436
MlyI GAGTC 3 cut(s) 2701, 3212, 3339
MmeI TCCRAC 3 cut(s) 1078, 1414, 3186
Mph1103I ATGCAT 3 cut(s) 1039, 1455, 3025
MroXI GAANNNNTTC 3 cut(s) 516, 1107, 2887
MslI CAYNNNNRTG 3 cut(s) 819, 2508, 3308
MspA1I CMGCKG 2 cut(s) 81, 1063
MspI CCGG 4 cut(s) 407, 2330, 2582, 3073
MunI CAATTG 4 cut(s) 186, 207, 917, 1469
Mva1269I GAATGC 2 cut(s) 278, 1643
MwoI GCNNNNNNNGC 6 cut(s) 1459, 1521, 1589, 1818, 2674, 3029
NcoI CCATGG 1 cut(s) 239
NdeI CATATG 1 cut(s) 1692
NlaIV GGNNCC 6 cut(s) 1536, 1705, 1904, 2964, 3097, 3098
NmuCI GTSAC 2 cut(s) 716, 2552
NsbI TGCGCA 1 cut(s) 419
NsiI ATGCAT 3 cut(s) 1039, 1455, 3025
NspI RCATGY 3 cut(s) 995, 1326, 1922
NspV TTCGAA 1 cut(s) 2680
OliI CACNNNNGTG 1 cut(s) 2508
PaeR7I CTCGAG 2 cut(s) 170, 1559
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 411
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 3 cut(s) 1062, 1167, 1500
PctI GAATGC 2 cut(s) 278, 1643
PdmI GAANNNNTTC 3 cut(s) 516, 1107, 2887
PfeI GAWTC 9 cut(s) 194, 325, 540, 575, 743, 966, 2014, 2513, 3142
PflMI CCANNNNNTGG 1 cut(s) 1391
PfoI TCCNGGA 1 cut(s) 2257
PkrI GCNGC 6 cut(s) 1144, 1177, 1464, 1820, 2597, 3182
PleI GAGTC 3 cut(s) 2701, 3211, 3338
PpsI GAGTC 3 cut(s) 2701, 3211, 3338
Ppu21I YACGTR 1 cut(s) 869
PpuMI RGGWCCY 2 cut(s) 1902, 3096
PshBI ATTAAT 2 cut(s) 1940, 2808
PsiI TTATAA 1 cut(s) 2972
Psp5II RGGWCCY 2 cut(s) 1902, 3096
PspN4I GGNNCC 6 cut(s) 1536, 1705, 1904, 2964, 3097, 3098
PspPI GGNCC 4 cut(s) 1090, 1703, 1902, 3096
PspPPI RGGWCCY 2 cut(s) 1902, 3096
PstI CTGCAG 2 cut(s) 2896, 3182
PstNI CAGNNNCTG 1 cut(s) 2556
PsuI RGATCY 3 cut(s) 1415, 2129, 3052
PvuII CAGCTG 1 cut(s) 1063
RsaI GTAC 8 cut(s) 121, 303, 339, 552, 602, 631, 1557, 1957
RsaNI GTAC 8 cut(s) 120, 302, 338, 551, 601, 630, 1556, 1956
RseI CAYNNNNRTG 3 cut(s) 819, 2508, 3308
SapI GCTCTTC 3 cut(s) 1062, 1167, 1500
SatI GCNGC 6 cut(s) 1143, 1176, 1463, 1819, 2596, 3181
Sau96I GGNCC 4 cut(s) 1090, 1703, 1902, 3096
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 3 cut(s) 2701, 3212, 3339
SduI GDGCHC 1 cut(s) 436
SexAI ACCWGGT 1 cut(s) 1092
SfcI CTRYAG 5 cut(s) 1755, 1813, 2172, 2892, 3178
Sfr274I CTCGAG 2 cut(s) 170, 1559
SfuI TTCGAA 1 cut(s) 2680
SinI GGWCC 3 cut(s) 1090, 1902, 3096
SlaI CTCGAG 2 cut(s) 170, 1559
SmiMI CAYNNNNRTG 3 cut(s) 819, 2508, 3308
SmlI CTYRAG 6 cut(s) 170, 728, 1559, 1664, 2246, 2285
SmoI CTYRAG 6 cut(s) 170, 728, 1559, 1664, 2246, 2285
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 2 cut(s) 81, 1538
SspI AATATT 1 cut(s) 2299
SspMI CTAG 6 cut(s) 537, 873, 911, 942, 1286, 1421
StuI AGGCCT 1 cut(s) 392
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 5 cut(s) 62, 634, 1429, 1867, 2558
TaiI ACGT 4 cut(s) 433, 723, 871, 1347
TaqI TCGA 9 cut(s) 171, 377, 1560, 2197, 2680, 2867, 2989, 3049, 3121
TatI WGTACW 4 cut(s) 119, 301, 1555, 1955
TfiI GAWTC 9 cut(s) 194, 325, 540, 575, 743, 966, 2014, 2513, 3142
TscAI CASTG 6 cut(s) 1634, 2557, 2932, 2953, 3112, 3310
TseFI GTSAC 2 cut(s) 716, 2552
TseI GCWGC 6 cut(s) 1142, 1175, 1462, 1818, 2595, 3180
Tsp45I GTSAC 2 cut(s) 716, 2552
TspDTI ATGAA 8 cut(s) 305, 399, 705, 1271, 1296, 1709, 2078, 2721
TspGWI ACGGA 3 cut(s) 1670, 3035, 3323
TspRI CASTG 6 cut(s) 1634, 2557, 2932, 2953, 3112, 3310
Van91I CCANNNNNTGG 1 cut(s) 1391
VpaK11BI GGWCC 3 cut(s) 1090, 1902, 3096
VspI ATTAAT 2 cut(s) 1940, 2808
XagI CCTNNNNNAGG 1 cut(s) 2892
XapI RAATTY 9 cut(s) 100, 1003, 1305, 1774, 2076, 2112, 2224, 2689, 2883
XceI RCATGY 3 cut(s) 995, 1326, 1922
XcmI CCANNNNNNNNNTGG 1 cut(s) 1990
XhoI CTCGAG 2 cut(s) 170, 1559
XmnI GAANNNNTTC 3 cut(s) 516, 1107, 2887
XspI CTAG 6 cut(s) 537, 873, 911, 942, 1286, 1421
ZrmI AGTACT 1 cut(s) 303
Zsp2I ATGCAT 3 cut(s) 1039, 1455, 3025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.