FvH4_3g07490

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000058
Physical Location & Seq
Forward (+)
4432825 .. 4437100
4276 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07490.t1

Sequence Viewer

Length: 3336 bp
ATGTCAAGCCGCACATTCTGCACAAATCCATTTTCGTTCCAGCATCCAACAATGGAGAGACTTACTTGGCTCTTCCTGGCCGCGGCTTGCTGCTTAACTATGGTGGCAGCACTAACCAACAGTAGTCTCACTACAGATGAGTCTGCTCTTCTTGCTCTCAAAGCTCGTATCACCAGCGACCCTGGTAACATCATCTCCACTAATTGGTCAACCACTACCCCTATTTGCAACTGGGTTGGTATTACTTGTGGTGCACGCCACTTTAGAGTCTCAGTCTTGAACCTCTCTTATATGGGACTCACGAGCACCATTCCTCCGGAACTTGGCAACCTATCGTTCCTTGTTGAGCTTAGCTTAAGAAACAACAGTTTTCATGGTGCCATGCCTTTGGAATTGGGACGTTTGCACCGATTGAAGCTCATCAGTTTCAGATTCAACAATTTTATGGGAGCCATTCCATCATGGTTTGGTTCCTTATCGAAACTCGAAACCTTCGACCTGTATGGTAATCAATTTTCAGGTTCCATACCTGCTGCCATCTTCAACTTAACAGCTCTCCAAGTAATCAGCCTGAGCAATAACCAGCTATCAGGTAGTATACCAAGAGAAATTGGAAACCTAACAAAGTTGAAGGAGATATACCTTGATAATAATAATTTCAGAGAAATTCCAAACGAGATTGGCATCTTGAATCAACTGGAGAAGTTGTTTGTGCAGTTCAATGACTTGCAAGGGCATGTTCCTCTTGCTGTCTTCAACATGTCTTCTTTGATCACTTTGACTTTCTATAGAAACAGTTTGAATGGTAGTCTTCCAAAGAATATATATCAACATCTTCCTAGCATTCAACAGTTGGATTTGGCTTACAACCAGATTGATGGTCCAATTCCATCCAATATTGGCAACTTAACCCAGATAAGGATGATTGACCTTGGCTGGAACTACTTGACAGGGACTATACCACATGAGATTGGTCATCTTCCAAGTTTAGATAGATTGGCCCTCGGAATTAATAATCTCGATGGCCTCATCCCATCCTCAATCTTCAACATTTCGACAATAACACTGATGGATCTTCACGACAATCAGCTCTCAGGCACCCTGCCAGCAAACGTAGGCCTTGGACTTCCAAACCTCATATTTTTGTTCATAGGAGGAAATACGCTTCGTGGAGTATTCTCTAAATCCATCTCCAATGCTTCTCAGCTCGTACGGCTAGAGCTTGGCTCAAACTTGTTTTCAGGCTTTATTCCTAGTGAGCTATGTGCCTTAACAAACCTTCGTCGGCTTACCTTACACCAGAATAATTTCACCATTGATACTTCTAGTCCAGGAGTAAATTTTTTCTCTTGTTTGGCAAGTCTTAAAGATCTGAGCACATTAGCCTTGTCAGACAATCCATTAAACTCCAAGCTTTCGGTTTCCTTTAGTAATCTCTCAGCATCAGTTGAATTCATTTACTTAGCTGACTGCAACATGAGAGGTAACATCCCCAATCATGTAGGCAACTTGACCAACTTAACACTCTTAGACCTTGCAGAAAATGAACTGAGTGGACCAATTCCAACTTCACTGGGAAGATTACCGAATGTCCAACTTCTGGATGTGCATGGTAATAAGTTGCAAGGACACATTCCAGATGAACTTTGTCAACTAAACCATCTAAATGAGTTGAGTCTGTATGAGAATCAACTTACTGGTGCCATACCTGCCTGCTTGGGTAATCTAAATGCAGCTCTAAGAGGTCTATTCTTACAGTCCAATTTGTTAACATCCACAATACCATCTACATTGTGGGGGCTTACAGATATCTTTCGCTTAAATCTTTCATCCAATTCTCTAGTTGGACCTCTTACAGAAGGTTTTGGCAATTTGAAAGTTGTCACAGATGTGGATTTATCAAACAACCAATTATCCGGTAGCATACCAAGTAGTATTGAGAGTCTACAAAATTTGGTCAATCTGTCCTTGGCAAGTAATAATTTAGAAGGCCTTATTCCTAGTTCGTTTGGGAACATGTTAAGCTTAGAACGTCTGGATTTATCCCAGAACAATCTATCTGGAATGATTCCAAAGTCTCTCGAAGCACTCTCATATCTCAAGTATCTGAACTTGTCTTTCAACAAACTCCAAGGAGAAATTCCAACAGGCGGACCTTTCACAAACTTGTCTGCTCAATCGTTTGTTTCAAACCGTGGACTCTGTGGTGTATACCGATTTCATGTTCAACCATGCAGAAAGAAAAGTGGTACATCTATCTTGAGATATGTTATTCCAGGGATCTTGTCAGCAATGTTTCTTTTGATCTCCATAATTTGGTTGATGCTACGAAAAAATAAGAATGTGGAAGCTACAATAGAAACTTCCTACTGGCCTCAACCCCTATACAGAAGGGTTTCATACCATGAGCTTCTAACTGCAACAAATGGATTCAGTGTGAGCAACTTACTTGGCATTGGCAGTTTTGGCTCAGTTTATAGAGGAACATTCTCAGACGGAGTAGATGTGGCCATAAAGGTTTTGAATTTAGAAACAGAAGGGGCTTTTACAAGTTTTGATGTTGAATGTGAAATGCTAAGCAATATTCGTCACCGAAATCTTATCAGAATCATCAGTTGCTGCTCTCAAAATGATTTCAAAGCTCTTATACTGAATTACATGCCTAATGGGAGCCTGGAGAAGTGGTTGTATTCTCATAACTGTTCTTTGAACTTGCTGAAAAGGTTGGATATCATGATAGATGTTGCATCAGCTTTGGAGTATCTTCATCATGATTATGAAACACCTATTGTTCATTGTGACTTGAAGCCCAACAATATTTTACTAGATGATGACATGGTTGCACATGTTGCTGATTTTGGCATAGCAAAACTATTAGGCACAGGAGATTCTATGACTCAAACGATGACGCTAGCGACCATTGGTTATATGGCTCCAGAGTACGGAAGAGAAGGGATTGTCACTAGAAGAGGGGATGTGTATAGTTTTGGGATTGTATTGATGGAAACATTCACAAGAAGGAAGCCAACAGATGAAATGTTTGTTGGGGAGATGAGTTTAAAGCAATGGGTTGCAAACTCACTATTTGCAGATGCAATAGCTAAAGTTGTTGATACCAATCTACTTGGAACACAGGAAGATAATGATACTGTTAGGAAGGAGTGTTTAGCATCCATTATGCGATTGGCTCTTTCATGTTCTGCCGAATCATCAGAAGAGAGGTTAAATATGCAAGAGGCTCTAGCCACTCTTCAGAAAATCAAGGTCAAGTTTTTGAAGGACAGGGAAGGAAATGTGCTGTTGAACCGCCCTCTTATTCACAAGAGCTTTAATTGA

Protein Analysis

1112

Amino Acids

122.5

Weight (kDa)

6.05

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 83 3.8e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 193 1.4e-06 Leucine-rich repeat region
LRR_8 PF13855 137 - 196 3.6e-07 Leucine rich repeat
LRR_8 PF13855 177 - 220 1.4e-06 Leucine rich repeat
LRR_14 PF23598 183 - 313 3.2e-11 Leucine-rich repeat region
LRR_4 PF12799 185 - 223 3.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 335 - 471 1.9e-06 Leucine-rich repeat region
LRR_14 PF23598 488 - 577 3.3e-06 Leucine-rich repeat region
LRR_14 PF23598 607 - 710 9e-08 Leucine-rich repeat region
LRR_8 PF13855 651 - 710 2e-11 Leucine rich repeat
Pkinase PF00069 812 - 1082 1.1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 813 - 1081 2.7e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 538, 1717
AccB1I GGYRCC 3 cut(s) 377, 1097, 1700
AccB7I CCANNNNNTGG 3 cut(s) 204, 1600, 2316
AccI GTMKAC 3 cut(s) 598, 1945, 2211
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 5 cut(s) 10, 81, 83, 2151, 3307
AclWI GGATC 2 cut(s) 1080, 2288
AcoI YGGCCR 2 cut(s) 78, 2508
AcsI RAATTY 6 cut(s) 666, 1339, 1451, 1951, 2139, 2524
AcuI CTGAAG 1 cut(s) 3236
AfaI GTAC 3 cut(s) 1212, 2251, 2942
AfiI CCNNNNNNNGG 8 cut(s) 82, 204, 323, 918, 1600, 2150, 2316, 2942
AflII CTTAAG 1 cut(s) 355
AflIII ACRYGT 3 cut(s) 759, 2016, 2845
AjnI CCWGG 5 cut(s) 75, 181, 1330, 2275, 2673
AjuI GAANNNNNNNTTGG 6 cut(s) 1552, 1584, 2124, 2156, 3027, 3059
AleI CACNNNNGTG 1 cut(s) 1889
Alw21I GWGCWC 3 cut(s) 256, 308, 1379
Alw26I GTCTC 4 cut(s) 52, 131, 274, 2082
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 2 cut(s) 1080, 2288
AlwNI CAGNNNCTG 1 cut(s) 2619
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 7 cut(s) 78, 999, 1024, 1117, 1990, 2372, 2508
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 5 cut(s) 90, 107, 533, 1733, 2619
ApoI RAATTY 6 cut(s) 666, 1339, 1451, 1951, 2139, 2524
AseI ATTAAT 1 cut(s) 1011
Asp700I GAANNNNTTC 2 cut(s) 1307, 2395
AspS9I GGNCC 5 cut(s) 881, 1000, 1556, 1847, 2153
AsuHPI GGTGA 3 cut(s) 163, 1303, 2582
AsuNHI GCTAGC 1 cut(s) 2911
AvaII GGWCC 4 cut(s) 881, 1556, 1847, 2153
BaeGI GKGCMC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 2510
BanI GGYRCC 3 cut(s) 377, 1097, 1700
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BauI CACGAG 1 cut(s) 301
BbsI GAAGAC 3 cut(s) 745, 756, 803
Bbv12I GWGCWC 3 cut(s) 256, 308, 1379
BbvI GCAGC 5 cut(s) 77, 119, 520, 1745, 2606
BceAI ACGGC 1 cut(s) 1229
BciT130I CCWGG 5 cut(s) 77, 183, 1332, 2277, 2675
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 4 cut(s) 52, 131, 274, 2082
BfmI CTRYAG 2 cut(s) 132, 787
BfrI CTTAAG 1 cut(s) 355
BfuAI ACCTGC 2 cut(s) 538, 1717
BglII AGATCT 1 cut(s) 1369
BisI GCNGC 8 cut(s) 10, 81, 84, 91, 108, 534, 1734, 2620
BlpI GCTNAGC 2 cut(s) 350, 2576
BlsI GCNGC 8 cut(s) 11, 82, 85, 92, 109, 535, 1735, 2621
Bme1390I CCNGG 5 cut(s) 77, 183, 1332, 2277, 2675
Bme18I GGWCC 4 cut(s) 881, 1556, 1847, 2153
BmgT120I GGNCC 5 cut(s) 881, 1000, 1556, 1847, 2153
BmiI GGNNCC 8 cut(s) 379, 451, 472, 523, 1099, 1702, 2672, 2934
BmrFI CCNGG 5 cut(s) 77, 183, 1332, 2277, 2675
BmrI ACTGGG 2 cut(s) 241, 1583
BmsI GCATC 7 cut(s) 52, 693, 1451, 2313, 2756, 3082, 3179
BmtI GCTAGC 1 cut(s) 2915
BmuI ACTGGG 2 cut(s) 241, 1583
BpiI GAAGAC 3 cut(s) 745, 756, 803
BplI GAGNNNNNCTC 2 cut(s) 1211, 1243
BpmI CTGGAG 3 cut(s) 719, 2696, 2919
Bpu10I CCTNAGC 1 cut(s) 572
Bpu1102I GCTNAGC 2 cut(s) 350, 2576
BpuEI CTTGAG 2 cut(s) 2084, 2281
BsaBI GATNNNNATC 2 cut(s) 683, 3117
BsaJI CCNNGG 9 cut(s) 81, 181, 931, 1003, 1120, 1968, 2131, 2194, 2276
BsaWI WCCGGW 2 cut(s) 316, 1916
Bsc4I CCNNNNNNNGG 8 cut(s) 82, 204, 323, 918, 1600, 2150, 2316, 2942
Bse1I ACTGG 5 cut(s) 236, 702, 1578, 1702, 2375
Bse3DI GCAATG 2 cut(s) 2298, 3071
Bse8I GATNNNNATC 2 cut(s) 683, 3117
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 5 cut(s) 77, 183, 1332, 2277, 2675
BseDI CCNNGG 9 cut(s) 81, 181, 931, 1003, 1120, 1968, 2131, 2194, 2276
BseJI GATNNNNATC 2 cut(s) 683, 3117
BseLI CCNNNNNNNGG 8 cut(s) 82, 204, 323, 918, 1600, 2150, 2316, 2942
BseMI GCAATG 2 cut(s) 2298, 3071
BseMII CTCAG 9 cut(s) 285, 563, 1107, 1217, 1364, 1452, 1541, 2484, 2505
BseNI ACTGG 5 cut(s) 236, 702, 1578, 1702, 2375
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 5 cut(s) 77, 119, 520, 1745, 2606
BsgI GTGCAG 2 cut(s) 4, 734
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 7 cut(s) 80, 1001, 1026, 1119, 1992, 2374, 2510
BshNI GGYRCC 3 cut(s) 377, 1097, 1700
BsiHKAI GWGCWC 3 cut(s) 256, 308, 1379
BsiSI CCGG 2 cut(s) 317, 1917
BsiWI CGTACG 1 cut(s) 1210
BslFI GGGAC 3 cut(s) 309, 411, 967
BslI CCNNNNNNNGG 8 cut(s) 82, 204, 323, 918, 1600, 2150, 2316, 2942
BsmAI GTCTC 4 cut(s) 52, 131, 274, 2082
BsmFI GGGAC 3 cut(s) 309, 411, 967
BsmI GAATGC 1 cut(s) 843
BsnI GGCC 7 cut(s) 80, 1001, 1026, 1119, 1992, 2374, 2510
Bsp1286I GDGCHC 3 cut(s) 256, 308, 1379
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
Bsp1720I GCTNAGC 2 cut(s) 350, 2576
BspACI CCGC 5 cut(s) 10, 81, 83, 2151, 3307
BspANI GGCC 7 cut(s) 80, 1001, 1026, 1119, 1992, 2374, 2510
BspCNI CTCAG 9 cut(s) 284, 564, 1106, 1216, 1365, 1451, 1542, 2483, 2504
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 2 cut(s) 2733, 2770
BspLI GGNNCC 8 cut(s) 379, 451, 472, 523, 1099, 1702, 2672, 2934
BspMI ACCTGC 2 cut(s) 538, 1717
BspOI GCTAGC 1 cut(s) 2915
BspPI GGATC 2 cut(s) 1080, 2288
BspQI GCTCTTC 2 cut(s) 77, 153
BspT107I GGYRCC 3 cut(s) 377, 1097, 1700
BspTI CTTAAG 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 2298, 3071
BsrI ACTGG 5 cut(s) 236, 702, 1578, 1702, 2375
BssECI CCNNGG 9 cut(s) 81, 181, 931, 1003, 1120, 1968, 2131, 2194, 2276
BssMI GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
BssNAI GTATAC 2 cut(s) 599, 2212
BssSI CACGAG 1 cut(s) 301
BssT1I CCWWGG 4 cut(s) 931, 1120, 1968, 2131
Bst1107I GTATAC 2 cut(s) 599, 2212
Bst2BI CACGAG 1 cut(s) 301
Bst2UI CCWGG 5 cut(s) 77, 183, 1332, 2277, 2675
Bst4CI ACNGT 8 cut(s) 122, 368, 797, 852, 1758, 2195, 2702, 3151
Bst6I CTCTTC 6 cut(s) 77, 153, 2941, 2962, 3210, 3255
BstAFI CTTAAG 1 cut(s) 355
BstAPI GCANNNNNTGC 2 cut(s) 18, 2849
BstC8I GCNNGC 5 cut(s) 88, 256, 1107, 1714, 2913
BstDSI CCRYGG 2 cut(s) 81, 2194
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 5 cut(s) 774, 1075, 1372, 2283, 2307
BstMAI GTCTC 4 cut(s) 52, 131, 274, 2082
BstMBI GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
BstMWI GCNNNNNNNGC 7 cut(s) 18, 152, 161, 1213, 1709, 2466, 2849
BstNI CCWGG 5 cut(s) 77, 183, 1332, 2277, 2675
BstNSI RCATGY 5 cut(s) 740, 763, 2020, 2662, 2849
BstSCI CCNGG 5 cut(s) 75, 181, 1330, 2275, 2673
BstSFI CTRYAG 2 cut(s) 132, 787
BstSLI GKGCMC 1 cut(s) 256
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 77, 119, 520, 1745, 2606
BstV2I GAAGAC 3 cut(s) 745, 756, 803
BstX2I RGATCY 3 cut(s) 1072, 1369, 2280
BstXI CCANNNNNNTGG 2 cut(s) 388, 878
BstYI RGATCY 3 cut(s) 1072, 1369, 2280
BstZ17I GTATAC 2 cut(s) 599, 2212
BsuRI GGCC 7 cut(s) 80, 1001, 1026, 1119, 1992, 2374, 2510
BtgI CCRYGG 2 cut(s) 81, 2194
BtsIMutI CAGTG 3 cut(s) 1064, 1571, 2440
BveI ACCTGC 2 cut(s) 538, 1717
Cac8I GCNNGC 5 cut(s) 88, 256, 1107, 1714, 2913
CaiI CAGNNNCTG 1 cut(s) 2619
CciI TCATGA 2 cut(s) 2733, 2770
Cfr13I GGNCC 5 cut(s) 881, 1000, 1556, 1847, 2153
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 1 cut(s) 2917
Csp6I GTAC 3 cut(s) 1211, 2250, 2941
CviQI GTAC 3 cut(s) 1211, 2250, 2941
DpnI GATC 5 cut(s) 773, 1074, 1371, 2282, 2306
DpnII GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
DraI TTTAAA 1 cut(s) 3060
EaeI YGGCCR 2 cut(s) 78, 2508
Eam1104I CTCTTC 6 cut(s) 77, 153, 2941, 2962, 3210, 3255
EarI CTCTTC 6 cut(s) 77, 153, 2941, 2962, 3210, 3255
EciI GGCGGA 1 cut(s) 2166
Eco130I CCWWGG 4 cut(s) 931, 1120, 1968, 2131
Eco147I AGGCCT 2 cut(s) 1119, 1992
Eco32I GATATC 2 cut(s) 1810, 2731
Eco47I GGWCC 4 cut(s) 881, 1556, 1847, 2153
Eco57I CTGAAG 1 cut(s) 3236
EcoRI GAATTC 1 cut(s) 1451
EcoRII CCWGG 5 cut(s) 75, 181, 1330, 2275, 2673
EcoRV GATATC 2 cut(s) 1810, 2731
EcoT14I CCWWGG 4 cut(s) 931, 1120, 1968, 2131
ErhI CCWWGG 4 cut(s) 931, 1120, 1968, 2131
FaqI GGGAC 3 cut(s) 309, 411, 967
FbaI TGATCA 1 cut(s) 771
FblI GTMKAC 3 cut(s) 598, 1945, 2211
Fnu4HI GCNGC 8 cut(s) 10, 81, 84, 91, 108, 534, 1734, 2620
Fsp4HI GCNGC 8 cut(s) 10, 81, 84, 91, 108, 534, 1734, 2620
GluI GCNGC 8 cut(s) 10, 81, 84, 91, 108, 534, 1734, 2620
GsuI CTGGAG 3 cut(s) 719, 2696, 2919
HaeIII GGCC 7 cut(s) 80, 1001, 1026, 1119, 1992, 2374, 2510
HapII CCGG 2 cut(s) 317, 1917
HgaI GACGC 1 cut(s) 2917
HincII GTYRAC 3 cut(s) 210, 1652, 1770
HindII GTYRAC 3 cut(s) 210, 1652, 1770
HindIII AAGCTT 2 cut(s) 1412, 2023
HpaI GTTAAC 1 cut(s) 1770
HpaII CCGG 2 cut(s) 317, 1917
HphI GGTGA 3 cut(s) 163, 1303, 2582
Hpy166II GTNNAC 9 cut(s) 210, 254, 599, 1556, 1652, 1770, 1946, 2198, 2212
Hpy8I GTNNAC 9 cut(s) 210, 254, 599, 1556, 1652, 1770, 1946, 2198, 2212
Hpy99I CGWCG 1 cut(s) 1287
HpyCH4III ACNGT 8 cut(s) 122, 368, 797, 852, 1758, 2195, 2702, 3151
HpyCH4IV ACGT 3 cut(s) 400, 1113, 2032
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 152, 161, 1213, 1709, 2466, 2849
HpySE526I ACGT 3 cut(s) 400, 1113, 2032
Kpn2I TCCGGA 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 771
KspAI GTTAAC 1 cut(s) 1770
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
LguI GCTCTTC 2 cut(s) 77, 153
LmnI GCTCC 3 cut(s) 449, 2670, 2938
Lsp1109I GCAGC 5 cut(s) 77, 119, 520, 1745, 2606
LweI GCATC 7 cut(s) 52, 693, 1451, 2313, 2756, 3082, 3179
MaeII ACGT 3 cut(s) 400, 1113, 2032
MaeIII GTNAC 6 cut(s) 185, 1484, 1882, 2588, 2798, 2959
MalI GATC 5 cut(s) 773, 1074, 1371, 2282, 2306
MboI GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
MflI RGATCY 3 cut(s) 1072, 1369, 2280
MhlI GDGCHC 3 cut(s) 256, 308, 1379
MlsI TGGCCA 1 cut(s) 2510
MluNI TGGCCA 1 cut(s) 2510
MlyI GAGTC 7 cut(s) 149, 276, 291, 1684, 1951, 2193, 2890
MmeI TCCRAC 7 cut(s) 71, 834, 1589, 1618, 1825, 2168, 2706
Mox20I TGGCCA 1 cut(s) 2510
MroI TCCGGA 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 1307, 2395
MscI TGGCCA 1 cut(s) 2510
MslI CAYNNNNRTG 3 cut(s) 1665, 1889, 2775
Msp20I TGGCCA 1 cut(s) 2510
MspA1I CMGCKG 1 cut(s) 83
MspCI CTTAAG 1 cut(s) 355
MspI CCGG 2 cut(s) 317, 1917
MspR9I CCNGG 5 cut(s) 77, 183, 1332, 2277, 2675
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 5 cut(s) 77, 183, 1332, 2277, 2675
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 7 cut(s) 18, 152, 161, 1213, 1709, 2466, 2849
NdeII GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
NheI GCTAGC 1 cut(s) 2911
NlaIV GGNNCC 8 cut(s) 379, 451, 472, 523, 1099, 1702, 2672, 2934
NmuCI GTSAC 4 cut(s) 1882, 2588, 2798, 2959
NspI RCATGY 5 cut(s) 740, 763, 2020, 2662, 2849
OliI CACNNNNGTG 1 cut(s) 1889
PagI TCATGA 2 cut(s) 2733, 2770
PceI AGGCCT 2 cut(s) 1119, 1992
PciI ACATGT 3 cut(s) 759, 2016, 2845
PciSI GCTCTTC 2 cut(s) 77, 153
PcsI WCGNNNNNNNCGW 2 cut(s) 406, 492
PctI GAATGC 1 cut(s) 843
PdmI GAANNNNTTC 2 cut(s) 1307, 2395
PfeI GAWTC 8 cut(s) 432, 691, 1687, 2068, 2430, 2607, 2888, 3206
Pfl23II CGTACG 1 cut(s) 1210
PflMI CCANNNNNTGG 3 cut(s) 204, 1600, 2316
PfoI TCCNGGA 1 cut(s) 1330
PkrI GCNGC 8 cut(s) 11, 82, 85, 92, 109, 535, 1735, 2621
PleI GAGTC 7 cut(s) 148, 275, 291, 1683, 1950, 2193, 2890
PpsI GAGTC 7 cut(s) 148, 275, 291, 1683, 1950, 2193, 2890
PscI ACATGT 3 cut(s) 759, 2016, 2845
PshBI ATTAAT 1 cut(s) 1011
Psp6I CCWGG 5 cut(s) 75, 181, 1330, 2275, 2673
PspGI CCWGG 5 cut(s) 75, 181, 1330, 2275, 2673
PspLI CGTACG 1 cut(s) 1210
PspN4I GGNNCC 8 cut(s) 379, 451, 472, 523, 1099, 1702, 2672, 2934
PspPI GGNCC 5 cut(s) 881, 1000, 1556, 1847, 2153
PstNI CAGNNNCTG 1 cut(s) 2619
PsuI RGATCY 3 cut(s) 1072, 1369, 2280
RsaI GTAC 3 cut(s) 1212, 2251, 2942
RsaNI GTAC 3 cut(s) 1211, 2250, 2941
RseI CAYNNNNRTG 3 cut(s) 1665, 1889, 2775
SacII CCGCGG 1 cut(s) 84
SapI GCTCTTC 2 cut(s) 77, 153
SatI GCNGC 8 cut(s) 10, 81, 84, 91, 108, 534, 1734, 2620
Sau3AI GATC 5 cut(s) 771, 1072, 1369, 2280, 2304
Sau96I GGNCC 5 cut(s) 881, 1000, 1556, 1847, 2153
SchI GAGTC 7 cut(s) 149, 276, 291, 1684, 1951, 2193, 2890
ScrFI CCNGG 5 cut(s) 77, 183, 1332, 2277, 2675
SduI GDGCHC 3 cut(s) 256, 308, 1379
SfaNI GCATC 7 cut(s) 52, 693, 1451, 2313, 2756, 3082, 3179
SfcI CTRYAG 2 cut(s) 132, 787
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 4 cut(s) 881, 1556, 1847, 2153
SmiMI CAYNNNNRTG 3 cut(s) 1665, 1889, 2775
SmlI CTYRAG 3 cut(s) 355, 2099, 2260
SmoI CTYRAG 3 cut(s) 355, 2099, 2260
SseBI AGGCCT 2 cut(s) 1119, 1992
SsiI CCGC 5 cut(s) 10, 81, 83, 2151, 3307
SspI AATATT 3 cut(s) 898, 2584, 2818
StuI AGGCCT 2 cut(s) 1119, 1992
StyD4I CCNGG 5 cut(s) 75, 181, 1330, 2275, 2673
StyI CCWWGG 4 cut(s) 931, 1120, 1968, 2131
TaaI ACNGT 8 cut(s) 122, 368, 797, 852, 1758, 2195, 2702, 3151
TaiI ACGT 3 cut(s) 403, 1116, 2035
TaqI TCGA 6 cut(s) 479, 486, 495, 1020, 1055, 2082
TauI GCSGC 3 cut(s) 12, 83, 86
TfiI GAWTC 8 cut(s) 432, 691, 1687, 2068, 2430, 2607, 2888, 3206
TscAI CASTG 3 cut(s) 1071, 1578, 2440
TseFI GTSAC 4 cut(s) 1882, 2588, 2798, 2959
TseI GCWGC 5 cut(s) 90, 107, 533, 1733, 2619
Tsp45I GTSAC 4 cut(s) 1882, 2588, 2798, 2959
TspGWI ACGGA 2 cut(s) 2511, 2958
TspRI CASTG 3 cut(s) 1071, 1578, 2440
Van91I CCANNNNNTGG 3 cut(s) 204, 1600, 2316
Vha464I CTTAAG 1 cut(s) 355
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 4 cut(s) 881, 1556, 1847, 2153
VspI ATTAAT 1 cut(s) 1011
XapI RAATTY 6 cut(s) 666, 1339, 1451, 1951, 2139, 2524
XceI RCATGY 5 cut(s) 740, 763, 2020, 2662, 2849
XcmI CCANNNNNNNNNTGG 3 cut(s) 1791, 2926, 3181
XmiI GTMKAC 3 cut(s) 598, 1945, 2211
XmnI GAANNNNTTC 2 cut(s) 1307, 2395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.