RchiOBHm_Chr6g0277761

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000058
Physical Location & Seq
Reverse (-)
40275116 .. 40280030
4915 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24919

Sequence Viewer

Length: 3297 bp
ATGGAGAGAGTTACTTGGTTCTTCCTGGTATACTTGATCTGTTGTACGGCGGCCAGGTGCTTAACTATAACGACACAAACCAAACTTAACATCACCACCGACGGCTCTGCTCTTCTTGCTCTCAAAGCCCGTATCACCAGTGACCCTCAAAACATTATCTTCACCAATTGGTCAACCATCACCCCTGTTTGCAACTGGGTTGGCGTCATTTGTGGTGCACACAATCATCGAGTCACAGAGTTGAACATCTCTTACTTTGGTCTCGTAGGCACCATTCCTCCTGAGCTAGGCAACTTATCATTGCTTGTTGATCTGGACATCCAAAATAATAGTTTCCAGGGTACCTTGCCTCAAGAATTAGCTCACCTGTGCCGGTTGAAGTTTATTAACTTGGGAAACAACAAGTTTATGGGAGTGATACCGTCATGGTTTGGGTCCTTGTACAAACTTCAAGGATTCAGGTTGTTTGGTAATCAATTTGTAGGTTCTATACCAGCTACCATCTTCAACTTATCTGCTCTGCAAATATTTAATCTGCGTTATAACCAACTCTCAGATATTCCAAATGAGATCGGCACCTTGAATCAGCTGGAAAAGTTGCTTGTGCAAAATAATGCCTTAAAAGGGCCTATTCCTATGGGTGTCTTCAATATCTCATCTTTGACTACGGCAAGTTTCTATGGAAACAACTTGAATGGTAGAATTCCCGACAATATATGTCAACATCTTCCAAATATTCAATACTTAAGTTTGGTTTACAATCAGTTTGATGGTCCACTTCCATCCAAGTTATGGCAATGCAAACAACTTCTTTTATTAGATTTGGGGTCTAACAAGTTCAGTGGAAGAATACCCAAATATATTGGGAACTTAACCCAAATAAAGATGATTTGGCTTGACACCAACAACTTAACAGGTACTGTACCATATGAGTTTGGTTATCTTCCAAATTTAGAGAAGTTGTTGCTCAGTGAGAATAATCTAAATGGCCATATCCCATCCTCAATCTTCAATATGTCCACAATAACATTATTAGATCTCTGCTTCAATCAGCTTTCAGGTAGCCTCCCAGCAATCATAGGCCTTACGCTTCCAAACCTAGAAGCGTTATCAGTAGGAGCGAATCACCTCAGTGGAGTAATCCCCAAATCCATCTCCAATGCTTCTGAGCTTCGGCATCTAAGCCTGGGAGCTAACTCTTTTTCTGGATTTATTCCAAGCACTCTGTGCCTCTTGACAAACCTTGAATTTCTCGAATTGGTTGGAATTAATTTGACCATTGATACCTCTACTCCAGAAGTTAATATTTTGTCTTGTCTAGCAAATCTTAGAAATTTGAGGTTTTTAGCCTTGGAAAGCAATCCATTACATTCCATGCTTCCAATTTCCTTCAGTAATTTCTCTACATCACTTGAATATCTTTATTTAGCAAAATGCAGCTTGAGAGGTAACATCCCCAGTGATATAGGGAATTTGAGTAGCTTGATAAAGTTATCCCTCGATGAGAATCAATTGAGTGGGCCAATTCCAATCTCAATGGGAAGACTACATAATCTTCAAGGTTTGTATATTTATGATAACACATTGCAAGGATACATTCCGGATGAACTTTGTCAACTAGAACACTTGAGTGATTTGATTTTGGGTGGGAATCAACTCTCTGGTTCTGTGCCTTCCTGCTTGGGTAATCTAACAGCATCTCTAAGAGATCTATCCTTATGGTCCAATTTATTGAATTCCACAATACCATCTACCTTGTGGGGACTTACAAATATCTTGGAAATAGACTTGTCATTCAATTCTGTAATCGGACCTCTATCAAGTGGTGTTGGTAATTTGAAAGTTGCTATAAACATAGATGTATCATACAACCAATTATCTGGTAGTATACCAAGCAGCATTGGGAGTTTATTGAATTTGGTCAATCTCTCCTTAGCACATAATAATCTAGAAGGCCCTATTCCTAGTTCCTTTGCTACATGTTCAAGCTTAGAACTATTGGATTTATCTCAGAACAGTCTCTCTGGAGTGATTCCAAAGTCTCTTGAAGCACTCTCATATCTCAAGTATCTGAATCTATCTTTCAACATACTCCAAGGAGAAATTCCAACCGGCGGTCCATTCAAAAACTTCCCTGCTGAATCATTTGTTTCAAACCGTGGACTCTGTGGTGCATCCCATTTTCAACTTCCACCATGCAAAACAAAAACTAGTATATTTATCTGGAAATATGCTATTCCCGGGATCTTGTCAGCAATGCTTCTAGCTACTTCCATATTTTGGATTTTGATGTTCCGCAGAAAAAAGAATGCGAATGCTGCAGCACATACTATCTTGACCCCTCAAGTTTTATGGAGACGAGTTTCGTACCAAGAGCTCCAAAGTGCAACAAATGGATTCAATGAAAGCAACTTACTTGGTACTGGGGGTTTTGGCTCAGTATATAAAGGAACATTGTCAGATGGGATAGACGTTGCGATAAAGGTTTTCAATTTGGAGTTAGAAGGGGCATTTACAAGTTTTGAGGTTGAATGTAAAGTGCTAAGCAATGTTCGTCACCGAAATCTTGTCAAAGTCATCAGTTGCTGCAGCCAAATTGATTTCAAAGGTCTTGTGTTGAATTACATGCCTAATGGGAGCCTTGAGAAGTGGTTGTATTCTCAGAATTCTTCTTTGAACATAATGCAGAGGTTGGATATAATGATAGATGTTGCATCAGCATTGGAGTACCTTCATCATGGTTATGAAGTAGCTATTGTCCATTGTGATTTGAAGCCGAGCAACATCCTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGTATTGCGAGACTCTTGGGTGAAGGAGATTCTATGACTCAAACCATGACCCTAGCTACAATTGGGTATATTGCTCCAGAGTATGGAATGGAAGGAATAGTGACCAAAAGAGGGGATGTGTATAGTTTTGGTATTGTAGTGATGGAAACATTCACAAGAAGGAAACCAACGGATGAGATCTTCGTTAAGGACATGAGATTAAGTCAATGGGTTGCAAATTTTCTAGTTGCAGATGCGATAGTTGAAGTTGTGGATGCTACTTTACTTGGAACAAATGAGGATCATGACTTCATGAGCAAGAGGGAGTGCTTATCATCCATTATGAGATTAGCTGTTGCTTGTTCAGCAGAATCACCGGAAGAAAGGATAAACATGCAAGATGCTGTAGCCACGCTTAAAAGAATCAAGATCAAGTTTACAAAGGACGCTGTTGCAGGAGGTTTGGTGTCGAACCGTCGTCCTGTTTCGTAA

Protein Analysis

1098

Amino Acids

120.52

Weight (kDa)

5.99

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 2.5e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 146 - 224 4.5e-06 Leucine-rich repeat region
LRR_8 PF13855 365 - 422 1.6e-06 Leucine rich repeat
LRR_14 PF23598 462 - 599 3.9e-08 Leucine-rich repeat region
LRR_8 PF13855 639 - 696 2.5e-09 Leucine rich repeat
Pkinase PF00069 801 - 1072 1.8e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 804 - 1073 7e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 3 cut(s) 269, 341, 575
AccB7I CCANNNNNTGG 3 cut(s) 792, 2280, 2909
AccI GTMKAC 2 cut(s) 30, 1888
AccIII TCCGGA 1 cut(s) 1600
AciI CCGC 3 cut(s) 50, 2115, 2296
AclWI GGATC 2 cut(s) 2252, 3114
AcoI YGGCCR 2 cut(s) 51, 988
AcuI CTGAAG 1 cut(s) 1375
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 1227
AfaI GTAC 8 cut(s) 46, 343, 443, 919, 924, 2369, 2422, 2729
AfiI CCNNNNNNNGG 7 cut(s) 287, 624, 792, 2114, 2280, 2909, 2937
AflII CTTAAG 1 cut(s) 745
AflIII ACRYGT 2 cut(s) 1979, 2812
AhlI ACTAGT 1 cut(s) 2210
AjnI CCWGG 4 cut(s) 24, 53, 336, 1185
AjuI GAANNNNNNNTTGG 2 cut(s) 2088, 2120
AleI CACNNNNGTG 2 cut(s) 1131, 1629
Alw21I GWGCWC 2 cut(s) 220, 2379
Alw26I GTCTC 5 cut(s) 266, 2024, 2046, 2350, 2830
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 2 cut(s) 2252, 3114
AlwNI CAGNNNCTG 2 cut(s) 920, 2586
Ama87I CYCGRG 1 cut(s) 2240
Aor13HI TCCGGA 1 cut(s) 1600
AoxI GGCC 6 cut(s) 51, 626, 988, 1081, 1520, 1954
ApaLI GTGCAC 1 cut(s) 216
ApeKI GCWGC 6 cut(s) 1437, 1896, 2318, 2321, 2586, 2589
AseI ATTAAT 1 cut(s) 1269
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 8 cut(s) 435, 626, 773, 1520, 1722, 1811, 1955, 2117
AsuC2I CCSGG 2 cut(s) 2241, 2242
AsuHPI GGTGA 9 cut(s) 85, 127, 154, 172, 356, 1118, 2549, 2858, 3171
AvaI CYCGRG 1 cut(s) 2240
AvaII GGWCC 5 cut(s) 435, 773, 1722, 1811, 2117
BaeGI GKGCMC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 990
BanI GGYRCC 3 cut(s) 269, 341, 575
BanII GRGCYC 1 cut(s) 2379
BbsI GAAGAC 2 cut(s) 637, 1549
Bbv12I GWGCWC 2 cut(s) 220, 2379
BbvI GCAGC 6 cut(s) 1449, 1908, 2305, 2333, 2573, 2601
BccI CCATC 9 cut(s) 185, 509, 764, 790, 1006, 1160, 1756, 2456, 2962
BceAI ACGGC 3 cut(s) 63, 118, 684
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 4 cut(s) 26, 55, 338, 1187
BciVI GTATCC 1 cut(s) 1586
BcnI CCSGG 2 cut(s) 2241, 2242
BcoDI GTCTC 5 cut(s) 266, 2024, 2046, 2350, 2830
BcuI ACTAGT 1 cut(s) 2210
BfmI CTRYAG 3 cut(s) 2319, 2587, 3210
BfrI CTTAAG 1 cut(s) 745
BfuI GTATCC 1 cut(s) 1586
BglII AGATCT 3 cut(s) 1036, 1708, 3003
BisI GCNGC 7 cut(s) 51, 1438, 1897, 2319, 2322, 2587, 2590
BlpI GCTNAGC 1 cut(s) 2543
BlsI GCNGC 7 cut(s) 52, 1439, 1898, 2320, 2323, 2588, 2591
Bme1390I CCNGG 6 cut(s) 26, 55, 338, 1187, 2241, 2242
Bme18I GGWCC 5 cut(s) 435, 773, 1722, 1811, 2117
BmeT110I CYCGRG 1 cut(s) 2240
BmgT120I GGNCC 8 cut(s) 435, 626, 773, 1520, 1722, 1811, 1955, 2117
BmiI GGNNCC 5 cut(s) 271, 343, 436, 577, 2639
BmrFI CCNGG 6 cut(s) 26, 55, 338, 1187, 2241, 2242
BmrI ACTGGG 3 cut(s) 205, 1452, 2433
BmsI GCATC 7 cut(s) 1186, 1706, 2183, 2723, 3049, 3070, 3196
BmuI ACTGGG 3 cut(s) 205, 1452, 2433
BpiI GAAGAC 2 cut(s) 637, 1549
BpmI CTGGAG 3 cut(s) 1278, 2046, 2886
Bpu10I CCTNAGC 2 cut(s) 282, 1933
Bpu1102I GCTNAGC 1 cut(s) 2543
BpuEI CTTGAG 6 cut(s) 336, 1462, 1648, 2048, 2328, 2663
BpuMI CCSGG 2 cut(s) 2241, 2242
BsaBI GATNNNNATC 1 cut(s) 1506
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 6 cut(s) 337, 1186, 1350, 2095, 2158, 2240
BsaWI WCCGGW 2 cut(s) 1600, 3181
BsaXI ACNNNNNCTCC 6 cut(s) 262, 292, 1276, 1306, 3255, 3285
Bsc4I CCNNNNNNNGG 7 cut(s) 287, 624, 792, 2114, 2280, 2909, 2937
Bse118I RCCGGY 2 cut(s) 372, 2111
Bse1I ACTGG 4 cut(s) 138, 200, 1458, 2428
Bse3DI GCAATG 5 cut(s) 299, 803, 1583, 2262, 2554
Bse8I GATNNNNATC 1 cut(s) 1506
BseAI TCCGGA 1 cut(s) 1600
BseBI CCWGG 4 cut(s) 26, 55, 338, 1187
BseDI CCNNGG 6 cut(s) 337, 1186, 1350, 2095, 2158, 2240
BseJI GATNNNNATC 1 cut(s) 1506
BseLI CCNNNNNNNGG 7 cut(s) 287, 624, 792, 2114, 2280, 2909, 2937
BseMI GCAATG 5 cut(s) 299, 803, 1583, 2262, 2554
BseMII CTCAG 8 cut(s) 273, 567, 982, 1144, 1158, 2024, 2451, 2675
BseNI ACTGG 4 cut(s) 138, 200, 1458, 2428
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 6 cut(s) 1449, 1908, 2305, 2333, 2573, 2601
BseYI CCCAGC 1 cut(s) 1069
BshFI GGCC 6 cut(s) 53, 628, 990, 1083, 1522, 1956
BshNI GGYRCC 3 cut(s) 269, 341, 575
BsiHKAI GWGCWC 2 cut(s) 220, 2379
BsiHKCI CYCGRG 1 cut(s) 2240
BsiSI CCGG 5 cut(s) 373, 1601, 2112, 2241, 3182
BslFI GGGAC 1 cut(s) 1776
BslI CCNNNNNNNGG 7 cut(s) 287, 624, 792, 2114, 2280, 2909, 2937
BsmAI GTCTC 5 cut(s) 266, 2024, 2046, 2350, 2830
BsmBI CGTCTC 1 cut(s) 2350
BsmFI GGGAC 1 cut(s) 1776
BsmI GAATGC 2 cut(s) 2314, 2320
BsnI GGCC 6 cut(s) 53, 628, 990, 1083, 1522, 1956
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 2240
Bsp1286I GDGCHC 2 cut(s) 220, 2379
Bsp13I TCCGGA 1 cut(s) 1600
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
Bsp1720I GCTNAGC 1 cut(s) 2543
BspACI CCGC 3 cut(s) 50, 2115, 2296
BspANI GGCC 6 cut(s) 53, 628, 990, 1083, 1522, 1956
BspCNI CTCAG 8 cut(s) 274, 566, 981, 1143, 1159, 2023, 2450, 2674
BspEI TCCGGA 1 cut(s) 1600
BspHI TCATGA 2 cut(s) 3109, 3117
BspLI GGNNCC 5 cut(s) 271, 343, 436, 577, 2639
BspMAI CTGCAG 2 cut(s) 2323, 2591
BspPI GGATC 2 cut(s) 2252, 3114
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 3 cut(s) 269, 341, 575
BspTI CTTAAG 1 cut(s) 745
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 5 cut(s) 299, 803, 1583, 2262, 2554
BsrFI RCCGGY 2 cut(s) 372, 2111
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 4 cut(s) 138, 200, 1458, 2428
BssAI RCCGGY 2 cut(s) 372, 2111
BssECI CCNNGG 6 cut(s) 337, 1186, 1350, 2095, 2158, 2240
BssMI GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
BssNAI GTATAC 2 cut(s) 31, 1889
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 1350, 2095
Bst1107I GTATAC 2 cut(s) 31, 1889
Bst2UI CCWGG 4 cut(s) 26, 55, 338, 1187
Bst4CI ACNGT 5 cut(s) 423, 922, 2018, 2159, 3281
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 745
BstAPI GCANNNNNTGC 2 cut(s) 1227, 2816
BstAUI TGTACA 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 2158
BstENI CCTNNNNNAGG 1 cut(s) 285
BstKTI GATC 9 cut(s) 39, 313, 573, 1039, 1711, 2247, 3006, 3109, 3237
BstMAI GTCTC 5 cut(s) 266, 2024, 2046, 2350, 2830
BstMBI GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
BstMWI GCNNNNNNNGC 6 cut(s) 116, 125, 1227, 2318, 2816, 3170
BstNI CCWGG 4 cut(s) 26, 55, 338, 1187
BstNSI RCATGY 4 cut(s) 1983, 2629, 2816, 3202
BstSCI CCNGG 6 cut(s) 24, 53, 336, 1185, 2239, 2240
BstSFI CTRYAG 3 cut(s) 2319, 2587, 3210
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 6 cut(s) 1449, 1908, 2305, 2333, 2573, 2601
BstV2I GAAGAC 2 cut(s) 637, 1549
BstX2I RGATCY 4 cut(s) 1036, 1708, 2244, 3003
BstXI CCANNNNNNTGG 1 cut(s) 1880
BstYI RGATCY 4 cut(s) 1036, 1708, 2244, 3003
BstZ17I GTATAC 2 cut(s) 31, 1889
BsuI GTATCC 1 cut(s) 1586
BsuRI GGCC 6 cut(s) 53, 628, 990, 1083, 1522, 1956
BtgI CCRYGG 1 cut(s) 2158
BtsIMutI CAGTG 5 cut(s) 145, 847, 976, 1138, 1465
CaiI CAGNNNCTG 2 cut(s) 920, 2586
CciI TCATGA 2 cut(s) 3109, 3117
Cfr10I RCCGGY 2 cut(s) 372, 2111
Cfr13I GGNCC 8 cut(s) 435, 626, 773, 1520, 1722, 1811, 1955, 2117
Cfr9I CCCGGG 1 cut(s) 2240
CseI GACGC 2 cut(s) 193, 3260
Csp6I GTAC 8 cut(s) 45, 342, 442, 918, 923, 2368, 2421, 2728
CspCI CAANNNNNGTGG 2 cut(s) 823, 858
CviQI GTAC 8 cut(s) 45, 342, 442, 918, 923, 2368, 2421, 2728
DpnI GATC 9 cut(s) 38, 312, 572, 1038, 1710, 2246, 3005, 3108, 3236
DpnII GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
DraIII CACNNNGTG 1 cut(s) 1227
EaeI YGGCCR 2 cut(s) 51, 988
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 2377
Eco130I CCWWGG 2 cut(s) 1350, 2095
Eco147I AGGCCT 1 cut(s) 1083
Eco24I GRGCYC 1 cut(s) 2379
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 5 cut(s) 435, 773, 1722, 1811, 2117
Eco53kI GAGCTC 1 cut(s) 2377
Eco57I CTGAAG 1 cut(s) 1375
Eco88I CYCGRG 1 cut(s) 2240
EcoICRI GAGCTC 1 cut(s) 2377
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO109I RGGNCCY 3 cut(s) 435, 626, 1955
EcoRI GAATTC 3 cut(s) 702, 1735, 2665
EcoRII CCWGG 4 cut(s) 24, 53, 336, 1185
EcoT14I CCWWGG 2 cut(s) 1350, 2095
EcoT38I GRGCYC 1 cut(s) 2379
ErhI CCWWGG 2 cut(s) 1350, 2095
Esp3I CGTCTC 1 cut(s) 2350
FaqI GGGAC 1 cut(s) 1776
FauNDI CATATG 1 cut(s) 928
FblI GTMKAC 2 cut(s) 30, 1888
Fnu4HI GCNGC 7 cut(s) 51, 1438, 1897, 2319, 2322, 2587, 2590
FriOI GRGCYC 1 cut(s) 2379
Fsp4HI GCNGC 7 cut(s) 51, 1438, 1897, 2319, 2322, 2587, 2590
GluI GCNGC 7 cut(s) 51, 1438, 1897, 2319, 2322, 2587, 2590
GsaI CCCAGC 1 cut(s) 1073
GsuI CTGGAG 3 cut(s) 1278, 2046, 2886
HaeIII GGCC 6 cut(s) 53, 628, 990, 1083, 1522, 1956
HapII CCGG 5 cut(s) 373, 1601, 2112, 2241, 3182
HgaI GACGC 2 cut(s) 193, 3260
Hin1I GRCGYC 1 cut(s) 204
HincII GTYRAC 3 cut(s) 174, 722, 1616
HindII GTYRAC 3 cut(s) 174, 722, 1616
HindIII AAGCTT 1 cut(s) 1987
HpaII CCGG 5 cut(s) 373, 1601, 2112, 2241, 3182
HphI GGTGA 9 cut(s) 85, 127, 154, 172, 356, 1118, 2549, 2858, 3171
Hpy188I TCNGA 7 cut(s) 556, 1168, 1811, 2013, 2073, 2461, 2664
Hpy99I CGWCG 2 cut(s) 104, 3285
HpyAV CCTTC 8 cut(s) 1399, 1683, 1946, 2498, 2741, 2843, 2912, 2979
HpyCH4III ACNGT 5 cut(s) 423, 922, 2018, 2159, 3281
HpyCH4IV ACGT 1 cut(s) 2472
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 125, 1227, 2318, 2816, 3170
HpySE526I ACGT 1 cut(s) 2472
Hsp92I GRCGYC 1 cut(s) 204
Kpn2I TCCGGA 1 cut(s) 1600
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 5 cut(s) 1118, 1190, 2382, 2637, 2905
Lsp1109I GCAGC 6 cut(s) 1449, 1908, 2305, 2333, 2573, 2601
LweI GCATC 7 cut(s) 1186, 1706, 2183, 2723, 3049, 3070, 3196
MaeII ACGT 1 cut(s) 2472
MaeIII GTNAC 6 cut(s) 10, 140, 232, 1448, 2555, 2926
MalI GATC 9 cut(s) 38, 312, 572, 1038, 1710, 2246, 3005, 3108, 3236
MboI GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
MfeI CAATTG 3 cut(s) 166, 1511, 2886
MflI RGATCY 4 cut(s) 1036, 1708, 2244, 3003
MhlI GDGCHC 2 cut(s) 220, 2379
MlsI TGGCCA 1 cut(s) 990
MluNI TGGCCA 1 cut(s) 990
MlyI GAGTC 4 cut(s) 240, 2157, 2832, 2857
MmeI TCCRAC 3 cut(s) 1243, 2132, 2673
Mox20I TGGCCA 1 cut(s) 990
MroI TCCGGA 1 cut(s) 1600
MscI TGGCCA 1 cut(s) 990
MslI CAYNNNNRTG 3 cut(s) 1131, 1629, 2742
Msp20I TGGCCA 1 cut(s) 990
MspA1I CMGCKG 1 cut(s) 589
MspCI CTTAAG 1 cut(s) 745
MspI CCGG 5 cut(s) 373, 1601, 2112, 2241, 3182
MspR9I CCNGG 6 cut(s) 26, 55, 338, 1187, 2241, 2242
MunI CAATTG 3 cut(s) 166, 1511, 2886
Mva1269I GAATGC 2 cut(s) 2314, 2320
MvaI CCWGG 4 cut(s) 26, 55, 338, 1187
MwoI GCNNNNNNNGC 6 cut(s) 116, 125, 1227, 2318, 2816, 3170
NciI CCSGG 2 cut(s) 2241, 2242
NdeI CATATG 1 cut(s) 928
NdeII GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
NlaIV GGNNCC 5 cut(s) 271, 343, 436, 577, 2639
NmeAIII GCCGAG 1 cut(s) 2802
NmuCI GTSAC 4 cut(s) 140, 232, 2555, 2926
NspI RCATGY 4 cut(s) 1983, 2629, 2816, 3202
OliI CACNNNNGTG 2 cut(s) 1131, 1629
PagI TCATGA 2 cut(s) 3109, 3117
PceI AGGCCT 1 cut(s) 1083
PciI ACATGT 2 cut(s) 1979, 2812
PciSI GCTCTTC 1 cut(s) 117
PctI GAATGC 2 cut(s) 2314, 2320
PflMI CCANNNNNTGG 3 cut(s) 792, 2280, 2909
PkrI GCNGC 7 cut(s) 52, 1439, 1898, 2320, 2323, 2588, 2591
PleI GAGTC 4 cut(s) 239, 2157, 2832, 2857
PpsI GAGTC 4 cut(s) 239, 2157, 2832, 2857
PpuMI RGGWCCY 1 cut(s) 435
PscI ACATGT 2 cut(s) 1979, 2812
PshBI ATTAAT 1 cut(s) 1269
PsiI TTATAA 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 2379
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 4 cut(s) 24, 53, 336, 1185
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 4 cut(s) 24, 53, 336, 1185
PspN4I GGNNCC 5 cut(s) 271, 343, 436, 577, 2639
PspPI GGNCC 8 cut(s) 435, 626, 773, 1520, 1722, 1811, 1955, 2117
PspPPI RGGWCCY 1 cut(s) 435
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstI CTGCAG 2 cut(s) 2323, 2591
PstNI CAGNNNCTG 2 cut(s) 920, 2586
PsuI RGATCY 4 cut(s) 1036, 1708, 2244, 3003
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 8 cut(s) 46, 343, 443, 919, 924, 2369, 2422, 2729
RsaNI GTAC 8 cut(s) 45, 342, 442, 918, 923, 2368, 2421, 2728
RseI CAYNNNNRTG 3 cut(s) 1131, 1629, 2742
SacI GAGCTC 1 cut(s) 2379
SapI GCTCTTC 1 cut(s) 117
SatI GCNGC 7 cut(s) 51, 1438, 1897, 2319, 2322, 2587, 2590
Sau3AI GATC 9 cut(s) 36, 310, 570, 1036, 1708, 2244, 3003, 3106, 3234
Sau96I GGNCC 8 cut(s) 435, 626, 773, 1520, 1722, 1811, 1955, 2117
SchI GAGTC 4 cut(s) 240, 2157, 2832, 2857
ScrFI CCNGG 6 cut(s) 26, 55, 338, 1187, 2241, 2242
SduI GDGCHC 2 cut(s) 220, 2379
SfaNI GCATC 7 cut(s) 1186, 1706, 2183, 2723, 3049, 3070, 3196
SfcI CTRYAG 3 cut(s) 2319, 2587, 3210
SinI GGWCC 5 cut(s) 435, 773, 1722, 1811, 2117
SmaI CCCGGG 1 cut(s) 2242
SmiMI CAYNNNNRTG 3 cut(s) 1131, 1629, 2742
SmlI CTYRAG 7 cut(s) 351, 745, 1441, 1627, 2063, 2343, 2642
SmoI CTYRAG 7 cut(s) 351, 745, 1441, 1627, 2063, 2343, 2642
SpeI ACTAGT 1 cut(s) 2210
SseBI AGGCCT 1 cut(s) 1083
SsiI CCGC 3 cut(s) 50, 2115, 2296
SspI AATATT 3 cut(s) 528, 736, 1306
SstI GAGCTC 1 cut(s) 2379
StuI AGGCCT 1 cut(s) 1083
StyD4I CCNGG 6 cut(s) 24, 53, 336, 1185, 2239, 2240
StyI CCWWGG 2 cut(s) 1350, 2095
TaaI ACNGT 5 cut(s) 423, 922, 2018, 2159, 3281
TaiI ACGT 1 cut(s) 2475
TaqI TCGA 4 cut(s) 229, 1254, 1500, 3275
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 53
TscAI CASTG 5 cut(s) 145, 847, 976, 1138, 1465
TseFI GTSAC 4 cut(s) 140, 232, 2555, 2926
TseI GCWGC 6 cut(s) 1437, 1896, 2318, 2321, 2586, 2589
Tsp45I GTSAC 4 cut(s) 140, 232, 2555, 2926
TspDTI ATGAA 5 cut(s) 1620, 2418, 2723, 2760, 3106
TspGWI ACGGA 1 cut(s) 3011
TspMI CCCGGG 1 cut(s) 2240
TspRI CASTG 5 cut(s) 145, 847, 976, 1138, 1465
Van91I CCANNNNNTGG 3 cut(s) 792, 2280, 2909
Vha464I CTTAAG 1 cut(s) 745
VneI GTGCAC 1 cut(s) 216
VpaK11BI GGWCC 5 cut(s) 435, 773, 1722, 1811, 2117
VspI ATTAAT 1 cut(s) 1269
XagI CCTNNNNNAGG 1 cut(s) 285
XbaI TCTAGA 1 cut(s) 1948
XceI RCATGY 4 cut(s) 1983, 2629, 2816, 3202
XcmI CCANNNNNNNNNTGG 2 cut(s) 789, 1755
XmaI CCCGGG 1 cut(s) 2240
XmiI GTMKAC 2 cut(s) 30, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.