Prupe.4G064600_v2.0.a1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000058
Physical Location & Seq
Reverse (-)
3106661 .. 3109968
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G064600.1

Sequence Viewer

Length: 2730 bp
ATGCTATCTGCGACTAATAATTATTCAATGATTTCAAGTTTTCAATCGGAAAGGAAAAACATTTTGAATTTGGAAGGAACTTCTCTGTTTTCCGTGCTCATGGAGAGAATTGGTTTCCTCTTGTCAATCACGTTTTTGCTCGTACTGCAGTACAGTTCTGTAGGCACAGTTGGGGTAGCACAAACCAACATCACCACGGACCAGTCTGCTCTTCTTGCTCTCAAATCCCATATCACTAGTTACCCTCACAACATCTTGTTCAACTGGTCAACCACCACATCCGTTTGCAATTGGGTTGGCGTTACTTGTGGTGCTCGTCACCTCAGAGTAGCATCATTGAATCTCTCGTACATGGGTCTCACAGGCACCATTCCACCGCACTTGGGCAACCTCTCGTTTCTCGTTGAGTTGCTCTTCATAAACAACAGTTTCCAAGGCACCTTGCCCCAGGAATTGGCTTATTTGCGTCGATTGAAGTTGATTAGCTTCAGATACAACAATTTTATGGGATCCATTCCATTGTGGTTTGGGTCCTTCCCCAAACTTCAAAGTATCGATTTGTGTGGCAATCAGTTTTCAGGTTCTATACCGGCGATTATCTTCAACTTATCTACACTACAAGTAATTAATCTAAGCAATAACCAACTATCAGGAAGTGTACCAAGAGAAATAAGGAACTTAACAATGCTCAAGGAGATAAACCTGAGCTACAACAAGTTCAACGAAATTCCAAACGAGATCGGCTCTTTAGATGAGTTGGAGAAGTTATATGTTCAGTCAAATGCCCTAAAAGGGCATGTTCCTCTGGGTGTTTTCAACATGTCTTCTTTGACTAATTTGAATCTACATGGAAACAACTTGAGTGGTAGTCTTCCTGACAATATATGCCAGCAGCTTCCTAGTCTTCAGGAGCTGGATCTGGGTCTCAACCAGTTTGATGGTCCACTTCCATCCAAATTATGGCAATGCACACAACTTCTTTTTCTATCATTGGAGGAAAACAATTTCAGTGGAAGCATACCCAGAAAAATTGGAAACTTAACCCAGTTAAGGGAGATTTATCTTGGCGTCAACAATTTGACAGGCATTATACCAAATGAGATGGGTGATCTTCAAAATTTGGAGTTATTGTCAATCGAACAGAATAATCTGAATGGCCTCATCCCATCCTCAATCTTCAACATCTCCAAGCTAAGAGCACTCTCGCTTACGCTGAATAAGCTATCGGGTAGCCTCCCAGCAAACATAGGCCTTGGGGTTCCAAACCTTCAACTCCTTTATATAGGAGCAACAGACGTCAGCGGAGTAATCCCCAACCTCTCCAATGCTTCTAAGCTCACCAGGATATCCATGAGCTACAACTCATTTACGGGGTTTATCCCGAGGACGCTCTGTGCCTTAATAAATCTTCAGTGGCTTGGCTTATACATGAATGATTTGACAATTGACACTTCCACTCTCTCTTGTTTGGCTAATCTTCAAAATTTGAAAGCACTAGACTTGGGAAATAATCCGTTAAATGTCAGACTTGACGATTCCTTTAGGAATTTCTCTTTGACATCATCACTTCAATATATTTCATTACACAATTGCATCATGAGGGGTAACATTCCCATTGGTATTGGCAACTTGAGCAGCTTGGTAGCCTTAGAACTGGGAGACAATCAATTCAGTGGATCAATTCCAACTTCAGTGGGAAGACTTGGGGATCTCCAAGGATTGAATTTGGGTGGTAACAAGTTGCAAGGCTACATCCCATATCAACTTTGTCAACTAGATAACCTAGCTTACTTATATTTGGGTAGTAATCAGCTCTCTGGTTCTATACCTTCCTGCTTGGGTAATCTAGCCCCATCTCTAAGAAGTCTATTACTGGAGTCCAATTCGTTGAGTTCCACAATACCATCTACTCTCTGGAGACTGACCTATATCTTGCATGTAAACTTATCATCCAATTCTCTAATTGGACCTCTCTCACAAGATATTGGAAACTTGAAAGTTGTCCTAGAGGTAGATTTATCAAATAACCATTTATCTGGTGTTATACCAAGCACCATTGGCACTCTTCGGGATCTCGTTAATCTATCCTTGGCAAATAATAATTTGGAAGGCCCTATTCCAAGTTCTTTTCACTACTTGCTAAGCCTGCAACTCTTGAATTTATCCAGAAACAATCTATCTGGAGTGATTCCCAAGTCTTTAGAAGCTCTGTCACTTCTCAAGCATCTGGATTTGTCTTTCAACAGGCTCCAAGGAGAAATTCCAACAGGTGGACCATTCCAAAACTTCAATGCTCAATCATTTGTCTCAAACAACGCACTTTGTGGTGCACCCCGACTTCATTTTCCACCATGCAAAAATACTACACTTGAACCAAATTGGAAGAAAGCTAAATATATCATCCCAGGGATTATATCAGTAATTCTCCTAGTGGCCTCCATATCTATGTTTGTACTACGCAGGAAAAGAAATGTGGAAGTTGCAGGAGAGGCTACCTCGTTGCCTCAACTTCTTTGGAGAAGAATTTCACACCAAGAACTTCTAAGGGCCACAAATGGATTTAACGAAAACAACTTACTCGGAAGTGGGGGTTTTGGCTCAGTATATAAAGGGACGCTTTCAGATGGAATAGACGTTGCAGTAAAGGTTTTCAGTTTACAGCTAGAAGGGGCTTTCAGGAGTTTTGATAGGGAATGTGAAATGCTAAGCAATATTCGTCATAGAAACCTT

Protein Analysis

910

Amino Acids

99.67

Weight (kDa)

8.31

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1299
AccB1I GGYRCC 2 cut(s) 365, 437
AccB7I CCANNNNNTGG 3 cut(s) 454, 960, 2270
AciI CCGC 2 cut(s) 377, 1302
AclWI GGATC 6 cut(s) 504, 517, 924, 1684, 1716, 2079
AcsI RAATTY 9 cut(s) 67, 726, 1117, 1483, 1546, 1723, 2158, 2259, 2523
AcuI CTGAAG 4 cut(s) 472, 890, 1394, 1674
AcyI GRCGYC 2 cut(s) 1068, 1296
AdeI CACNNNGTG 1 cut(s) 2324
AfaI GTAC 5 cut(s) 144, 152, 350, 660, 2454
AfiI CCNNNNNNNGG 7 cut(s) 383, 454, 792, 960, 1050, 1051, 2270
AflIII ACRYGT 1 cut(s) 819
AhlI ACTAGT 1 cut(s) 236
AjnI CCWGG 3 cut(s) 447, 1340, 2404
AjuI GAANNNNNNNTTGG 2 cut(s) 2244, 2276
Alw21I GWGCWC 4 cut(s) 99, 316, 1201, 2332
Alw26I GTCTC 5 cut(s) 362, 929, 1653, 1912, 2311
Alw44I GTGCAC 1 cut(s) 2328
AlwI GGATC 6 cut(s) 504, 517, 924, 1684, 1716, 2079
AlwNI CAGNNNCTG 1 cut(s) 913
Ama87I CYCGRG 1 cut(s) 1381
AoxI GGCC 5 cut(s) 1156, 1249, 2110, 2433, 2547
ApaLI GTGCAC 1 cut(s) 2328
ApeKI GCWGC 2 cut(s) 892, 1635
ApoI RAATTY 9 cut(s) 67, 726, 1117, 1483, 1546, 1723, 2158, 2259, 2523
AseI ATTAAT 1 cut(s) 627
Asp700I GAANNNNTTC 1 cut(s) 2524
AspS9I GGNCC 7 cut(s) 199, 531, 941, 1967, 2111, 2273, 2547
AsuHPI GGTGA 4 cut(s) 184, 311, 1118, 1330
AvaI CYCGRG 1 cut(s) 1381
AvaII GGWCC 5 cut(s) 199, 531, 941, 1967, 2273
BaeGI GKGCMC 1 cut(s) 2332
BamHI GGATCC 1 cut(s) 509
BanI GGYRCC 2 cut(s) 365, 437
BbsI GAAGAC 4 cut(s) 816, 863, 896, 1705
Bbv12I GWGCWC 4 cut(s) 99, 316, 1201, 2332
BbvI GCAGC 2 cut(s) 904, 1647
BccI CCATC 7 cut(s) 932, 958, 1096, 1174, 1861, 1912, 2618
BciT130I CCWGG 3 cut(s) 449, 1342, 2406
BcoDI GTCTC 5 cut(s) 362, 929, 1653, 1912, 2311
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 9 cut(s) 237, 900, 1496, 1775, 1784, 1847, 2006, 2429, 2663
BfmI CTRYAG 2 cut(s) 146, 159
BisI GCNGC 2 cut(s) 893, 1636
BlpI GCTNAGC 2 cut(s) 2141, 2705
BlsI GCNGC 2 cut(s) 894, 1637
Bme1390I CCNGG 3 cut(s) 449, 1342, 2406
Bme18I GGWCC 5 cut(s) 199, 531, 941, 1967, 2273
BmeT110I CYCGRG 1 cut(s) 1381
BmgT120I GGNCC 7 cut(s) 199, 531, 941, 1967, 2111, 2273, 2547
BmiI GGNNCC 6 cut(s) 367, 439, 511, 532, 1260, 2249
BmrFI CCNGG 3 cut(s) 449, 1342, 2406
BmrI ACTGGG 2 cut(s) 1039, 1664
BmsI GCATC 3 cut(s) 341, 1602, 2233
BmuI ACTGGG 2 cut(s) 1039, 1664
BpiI GAAGAC 4 cut(s) 816, 863, 896, 1705
BplI GAGNNNNNCTC 4 cut(s) 728, 760, 2480, 2512
BpmI CTGGAG 3 cut(s) 1895, 1936, 2202
Bpu10I CCTNAGC 1 cut(s) 704
Bpu1102I GCTNAGC 2 cut(s) 2141, 2705
BpuEI CTTGAG 4 cut(s) 674, 880, 1651, 2204
Bsa29I ATCGAT 1 cut(s) 555
BsaHI GRCGYC 2 cut(s) 1068, 1296
BsaI GGTCTC 2 cut(s) 362, 929
BsaXI ACNNNNNCTCC 2 cut(s) 1115, 1145
Bsc4I CCNNNNNNNGG 7 cut(s) 383, 454, 792, 960, 1050, 1051, 2270
Bse118I RCCGGY 1 cut(s) 589
Bse1I ACTGG 6 cut(s) 202, 269, 931, 1045, 1659, 1878
Bse3DI GCAATG 1 cut(s) 971
BseBI CCWGG 3 cut(s) 449, 1342, 2406
BseCI ATCGAT 1 cut(s) 555
BseGI GGATG 7 cut(s) 278, 950, 1161, 1166, 1752, 1949, 2400
BseLI CCNNNNNNNGG 7 cut(s) 383, 454, 792, 960, 1050, 1051, 2270
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 3 cut(s) 337, 695, 2613
BseNI ACTGG 6 cut(s) 202, 269, 931, 1045, 1659, 1878
BseSI GKGCMC 1 cut(s) 2332
BseXI GCAGC 2 cut(s) 904, 1647
BseYI CCCAGC 1 cut(s) 1237
BshFI GGCC 5 cut(s) 1158, 1251, 2112, 2435, 2549
BshNI GGYRCC 2 cut(s) 365, 437
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 4 cut(s) 99, 316, 1201, 2332
BsiHKCI CYCGRG 1 cut(s) 1381
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 2626
BslI CCNNNNNNNGG 7 cut(s) 383, 454, 792, 960, 1050, 1051, 2270
BsmAI GTCTC 5 cut(s) 362, 929, 1653, 1912, 2311
BsmFI GGGAC 1 cut(s) 2626
BsnI GGCC 5 cut(s) 1158, 1251, 2112, 2435, 2549
Bso31I GGTCTC 2 cut(s) 362, 929
BsoBI CYCGRG 1 cut(s) 1381
Bsp1286I GDGCHC 4 cut(s) 99, 316, 1201, 2332
Bsp143I GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
Bsp1720I GCTNAGC 2 cut(s) 2141, 2705
BspACI CCGC 2 cut(s) 377, 1302
BspANI GGCC 5 cut(s) 1158, 1251, 2112, 2435, 2549
BspCNI CTCAG 3 cut(s) 336, 696, 2612
BspDI ATCGAT 1 cut(s) 555
BspHI TCATGA 1 cut(s) 1596
BspLI GGNNCC 6 cut(s) 367, 439, 511, 532, 1260, 2249
BspMAI CTGCAG 1 cut(s) 150
BspPI GGATC 6 cut(s) 504, 517, 924, 1684, 1716, 2079
BspQI GCTCTTC 2 cut(s) 216, 419
BspT107I GGYRCC 2 cut(s) 365, 437
BspTNI GGTCTC 2 cut(s) 362, 929
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 589
BsrI ACTGG 6 cut(s) 202, 269, 931, 1045, 1659, 1878
BssAI RCCGGY 1 cut(s) 589
BssMI GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
BssNI GRCGYC 2 cut(s) 1068, 1296
BssT1I CCWWGG 5 cut(s) 433, 1252, 1714, 2088, 2251
Bst2UI CCWGG 3 cut(s) 449, 1342, 2406
Bst4CI ACNGT 3 cut(s) 155, 169, 428
Bst6I CTCTTC 3 cut(s) 216, 419, 2070
BstACI GRCGYC 2 cut(s) 1068, 1296
BstC8I GCNNGC 2 cut(s) 890, 2147
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 7 cut(s) 278, 950, 1161, 1166, 1752, 1949, 2400
BstKTI GATC 7 cut(s) 512, 741, 919, 1111, 1679, 1711, 2074
BstMAI GTCTC 5 cut(s) 362, 929, 1653, 1912, 2311
BstMBI GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
BstMWI GCNNNNNNNGC 7 cut(s) 145, 215, 1219, 1632, 2058, 2146, 2489
BstNI CCWGG 3 cut(s) 449, 1342, 2406
BstNSI RCATGY 3 cut(s) 800, 823, 1940
BstSCI CCNGG 3 cut(s) 447, 1340, 2404
BstSFI CTRYAG 2 cut(s) 146, 159
BstSLI GKGCMC 1 cut(s) 2332
BstV1I GCAGC 2 cut(s) 904, 1647
BstV2I GAAGAC 4 cut(s) 816, 863, 896, 1705
BstX2I RGATCY 4 cut(s) 509, 916, 1708, 2071
BstXI CCANNNNNNTGG 2 cut(s) 938, 2036
BstYI RGATCY 4 cut(s) 509, 916, 1708, 2071
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 5 cut(s) 1158, 1251, 2112, 2435, 2549
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 7 cut(s) 278, 950, 1161, 1166, 1752, 1949, 2400
BtsIMutI CAGTG 4 cut(s) 1015, 1418, 1678, 1698
Cac8I GCNNGC 2 cut(s) 890, 2147
CaiI CAGNNNCTG 1 cut(s) 913
CciI TCATGA 1 cut(s) 1596
Cfr10I RCCGGY 1 cut(s) 589
Cfr13I GGNCC 7 cut(s) 199, 531, 941, 1967, 2111, 2273, 2547
ClaI ATCGAT 1 cut(s) 555
CseI GACGC 4 cut(s) 455, 1057, 1396, 2623
Csp6I GTAC 5 cut(s) 143, 151, 349, 659, 2453
CspCI CAANNNNNGTGG 8 cut(s) 363, 398, 844, 879, 991, 1026, 1674, 1709
CviQI GTAC 5 cut(s) 143, 151, 349, 659, 2453
DpnI GATC 7 cut(s) 511, 740, 918, 1110, 1678, 1710, 2073
DpnII GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
DraIII CACNNNGTG 1 cut(s) 2324
Eam1104I CTCTTC 3 cut(s) 216, 419, 2070
EarI CTCTTC 3 cut(s) 216, 419, 2070
Eco130I CCWWGG 5 cut(s) 433, 1252, 1714, 2088, 2251
Eco147I AGGCCT 1 cut(s) 1251
Eco31I GGTCTC 2 cut(s) 362, 929
Eco32I GATATC 1 cut(s) 1347
Eco47I GGWCC 5 cut(s) 199, 531, 941, 1967, 2273
Eco57I CTGAAG 4 cut(s) 472, 890, 1394, 1674
Eco88I CYCGRG 1 cut(s) 1381
EcoO109I RGGNCCY 2 cut(s) 531, 2111
EcoRII CCWGG 3 cut(s) 447, 1340, 2404
EcoRV GATATC 1 cut(s) 1347
EcoT14I CCWWGG 5 cut(s) 433, 1252, 1714, 2088, 2251
ErhI CCWWGG 5 cut(s) 433, 1252, 1714, 2088, 2251
FalI AAGNNNNNCTT 2 cut(s) 2601, 2633
FaqI GGGAC 1 cut(s) 2626
Fnu4HI GCNGC 2 cut(s) 893, 1636
FokI GGATG 7 cut(s) 265, 937, 1148, 1153, 1739, 1936, 2387
Fsp4HI GCNGC 2 cut(s) 893, 1636
FspBI CTAG 9 cut(s) 237, 900, 1496, 1775, 1784, 1847, 2006, 2429, 2663
GluI GCNGC 2 cut(s) 893, 1636
GsaI CCCAGC 1 cut(s) 1241
GsuI CTGGAG 3 cut(s) 1895, 1936, 2202
HaeIII GGCC 5 cut(s) 1158, 1251, 2112, 2435, 2549
HapII CCGG 1 cut(s) 590
HgaI GACGC 4 cut(s) 455, 1057, 1396, 2623
Hin1I GRCGYC 2 cut(s) 1068, 1296
HincII GTYRAC 3 cut(s) 270, 1072, 1772
HindII GTYRAC 3 cut(s) 270, 1072, 1772
HinfI GANTC 5 cut(s) 340, 841, 1535, 1877, 2188
HpaII CCGG 1 cut(s) 590
HphI GGTGA 4 cut(s) 184, 311, 1118, 1330
Hpy166II GTNNAC 9 cut(s) 270, 659, 944, 1072, 1772, 1942, 2273, 2330, 2657
Hpy188I TCNGA 7 cut(s) 49, 326, 491, 1152, 1526, 2582, 2623
Hpy8I GTNNAC 9 cut(s) 270, 659, 944, 1072, 1772, 1942, 2273, 2330, 2657
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 6 cut(s) 68, 544, 1277, 1839, 2102, 2660
HpyCH4III ACNGT 3 cut(s) 155, 169, 428
HpyCH4IV ACGT 3 cut(s) 131, 1296, 2634
HpyF10VI GCNNNNNNNGC 7 cut(s) 145, 215, 1219, 1632, 2058, 2146, 2489
HpySE526I ACGT 3 cut(s) 131, 1296, 2634
Hsp92I GRCGYC 2 cut(s) 1068, 1296
Kzo9I GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
LguI GCTCTTC 2 cut(s) 216, 419
LmnI GCTCC 3 cut(s) 910, 1286, 2253
Lsp1109I GCAGC 2 cut(s) 904, 1647
LweI GCATC 3 cut(s) 341, 1602, 2233
MaeI CTAG 9 cut(s) 237, 900, 1496, 1775, 1784, 1847, 2006, 2429, 2663
MaeII ACGT 3 cut(s) 131, 1296, 2634
MaeIII GTNAC 6 cut(s) 239, 301, 317, 1604, 1733, 2211
MalI GATC 7 cut(s) 511, 740, 918, 1110, 1678, 1710, 2073
MboI GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
MfeI CAATTG 3 cut(s) 289, 1443, 1588
MflI RGATCY 4 cut(s) 509, 916, 1708, 2071
MhlI GDGCHC 4 cut(s) 99, 316, 1201, 2332
MlyI GAGTC 1 cut(s) 1886
MmeI TCCRAC 3 cut(s) 738, 1709, 2288
MroXI GAANNNNTTC 1 cut(s) 2524
MseI TTAA 8 cut(s) 627, 680, 1040, 1049, 1400, 1517, 2079, 2562
MslI CAYNNNNRTG 1 cut(s) 2444
MspA1I CMGCKG 1 cut(s) 1302
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 3 cut(s) 449, 1342, 2406
MunI CAATTG 3 cut(s) 289, 1443, 1588
MvaI CCWGG 3 cut(s) 449, 1342, 2406
MwoI GCNNNNNNNGC 7 cut(s) 145, 215, 1219, 1632, 2058, 2146, 2489
NdeII GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
NlaIV GGNNCC 6 cut(s) 367, 439, 511, 532, 1260, 2249
NmuCI GTSAC 2 cut(s) 317, 2211
NspI RCATGY 3 cut(s) 800, 823, 1940
PagI TCATGA 1 cut(s) 1596
PasI CCCWGGG 1 cut(s) 2405
PceI AGGCCT 1 cut(s) 1251
PciI ACATGT 1 cut(s) 819
PciSI GCTCTTC 2 cut(s) 216, 419
PdmI GAANNNNTTC 1 cut(s) 2524
PfeI GAWTC 4 cut(s) 340, 841, 1535, 2188
PflMI CCANNNNNTGG 3 cut(s) 454, 960, 2270
PkrI GCNGC 2 cut(s) 894, 1637
PleI GAGTC 1 cut(s) 1885
PpsI GAGTC 1 cut(s) 1885
PpuMI RGGWCCY 1 cut(s) 531
PscI ACATGT 1 cut(s) 819
PshBI ATTAAT 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 531
Psp6I CCWGG 3 cut(s) 447, 1340, 2404
PspFI CCCAGC 1 cut(s) 1237
PspGI CCWGG 3 cut(s) 447, 1340, 2404
PspN4I GGNNCC 6 cut(s) 367, 439, 511, 532, 1260, 2249
PspPI GGNCC 7 cut(s) 199, 531, 941, 1967, 2111, 2273, 2547
PspPPI RGGWCCY 1 cut(s) 531
PstI CTGCAG 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 913
PsuI RGATCY 4 cut(s) 509, 916, 1708, 2071
RsaI GTAC 5 cut(s) 144, 152, 350, 660, 2454
RsaNI GTAC 5 cut(s) 143, 151, 349, 659, 2453
RseI CAYNNNNRTG 1 cut(s) 2444
SapI GCTCTTC 2 cut(s) 216, 419
SaqAI TTAA 8 cut(s) 627, 680, 1040, 1049, 1400, 1517, 2079, 2562
SatI GCNGC 2 cut(s) 893, 1636
Sau3AI GATC 7 cut(s) 509, 738, 916, 1108, 1676, 1708, 2071
Sau96I GGNCC 7 cut(s) 199, 531, 941, 1967, 2111, 2273, 2547
SchI GAGTC 1 cut(s) 1886
ScrFI CCNGG 3 cut(s) 449, 1342, 2406
SduI GDGCHC 4 cut(s) 99, 316, 1201, 2332
SfaNI GCATC 3 cut(s) 341, 1602, 2233
SfcI CTRYAG 2 cut(s) 146, 159
SinI GGWCC 5 cut(s) 199, 531, 941, 1967, 2273
SmiMI CAYNNNNRTG 1 cut(s) 2444
SmlI CTYRAG 4 cut(s) 689, 859, 1630, 2219
SmoI CTYRAG 4 cut(s) 689, 859, 1630, 2219
SpeI ACTAGT 1 cut(s) 236
SseBI AGGCCT 1 cut(s) 1251
SsiI CCGC 2 cut(s) 377, 1302
SspI AATATT 1 cut(s) 2713
SspMI CTAG 9 cut(s) 237, 900, 1496, 1775, 1784, 1847, 2006, 2429, 2663
StuI AGGCCT 1 cut(s) 1251
StyD4I CCNGG 3 cut(s) 447, 1340, 2404
StyI CCWWGG 5 cut(s) 433, 1252, 1714, 2088, 2251
TaaI ACNGT 3 cut(s) 155, 169, 428
TaiI ACGT 3 cut(s) 134, 1299, 2637
TaqI TCGA 3 cut(s) 469, 555, 1137
TatI WGTACW 2 cut(s) 150, 2452
TfiI GAWTC 4 cut(s) 340, 841, 1535, 2188
Tru1I TTAA 8 cut(s) 627, 680, 1040, 1049, 1400, 1517, 2079, 2562
Tru9I TTAA 8 cut(s) 627, 680, 1040, 1049, 1400, 1517, 2079, 2562
TscAI CASTG 4 cut(s) 1015, 1418, 1678, 1698
TseFI GTSAC 2 cut(s) 317, 2211
TseI GCWGC 2 cut(s) 892, 1635
Tsp45I GTSAC 2 cut(s) 317, 2211
TspDTI ATGAA 4 cut(s) 406, 1445, 1569, 2330
TspGWI ACGGA 4 cut(s) 82, 212, 271, 1503
TspRI CASTG 4 cut(s) 1015, 1418, 1678, 1698
Van91I CCANNNNNTGG 3 cut(s) 454, 960, 2270
VneI GTGCAC 1 cut(s) 2328
VpaK11BI GGWCC 5 cut(s) 199, 531, 941, 1967, 2273
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 9 cut(s) 67, 726, 1117, 1483, 1546, 1723, 2158, 2259, 2523
XceI RCATGY 3 cut(s) 800, 823, 1940
XcmI CCANNNNNNNNNTGG 2 cut(s) 957, 1911
XmnI GAANNNNTTC 1 cut(s) 2524
XspI CTAG 9 cut(s) 237, 900, 1496, 1775, 1784, 1847, 2006, 2429, 2663
ZraI GACGTC 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.