MD10G1277600.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000058
Physical Location & Seq
Forward (+)
36833050 .. 36836764
3715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1277600.v1.1.491

Sequence Viewer

Length: 2505 bp
ATGTCGTCTTTGAAAATTTTGGCTCTATCAGTTAACAAAATGACTGGTCGTATTCCAGACAATTTATGTCAACATCTTCCAAGTATTCAGATCTTGAGTTTGGCTGGCAACAATTTTGATGGTCCACTTCCATCCAAATTATGGCAATGCAAAGAGCTTCTTACGCTCTTATTAAGCAACAATCACTTGACAGGTACGATACCAGATGAGATTGGTGATCTTCAAAATTTACAAATTTTACAACTCGATTTTAATAATCTTAATGGTCCTATCCCACGCAAAATCTTCAATATGTCCATGTTGAGACGAATTTCACTTGTTGATAATCAACTCTCAGGAAGTCTTCCAACAGACATAGCCATTGGAGTTCCAAACCTAGAATTCATGTTTGTTGGAATGAATAATCTCAGTGGACGAATCCCCAACTTTATCTCCAATGGCTCTAAGCTCACGAGGCTAGACATTACCTTAGCTTACACAAACAATTTAACGATTGATACTTCAACCCTAGAAGTAAACATCCTTTCTTGTTTGGATAATCTTCGAAATCTTTGGGGACTCTACTTGTCTAACAATCCATTAAATGCCACTCTTCCTGCTTCCCTTGGAAATTTCTCTACGTCGCTTGACTATCTTCATTTAGGATTTTGCAATTTGAGGGGTAGCTTTCCCAATGATATTGGAAACTTCAGTGGCTTGATATCCTTAACGATGGGATCCAATCAATTGAGTGGGTCAATTCCAACTTCTGTGGGAAGACTAAGGAATCTCCAGGGTCTCAGTTTGCATGATAACAAGTTGCAAGGATACATCCCAGATGAACTTTGCCAACTGGACAACCTAGTTGAATTGAATATCAGTGGTAATCAACTCTCTGGTTCCGTACCTTCCTGCCTAGGTAATCTAACTGCAAAATTTAGAAAGATATCATTAGAGTCCAATTTGTTAACTTCCACAATACCATCTACCTTATGGAAGCTTACATATATATTGATCCTAGACTTGTCATCCAATTCACTCGTTGGTCCTCTCTCAGAAGATGTTGGAAAACTGAAAGTTGTGACAGAAGTGAATTTATCAAATAACCATTTATATGGTGCTATACCAAGCAGTATGAGTGGACTCCAGGATTTGGTTAGTCTCTCCTTGGAATATAATAATTTAGAAGGTCAAATCCCCAGTTCATTTGGAAACTTGCTAAGCCTGGAACTCTTGGATCTATCCAAAAACAATCTTTCCGGAGTAATTCCAAAGTCTCTTGAAGCACTCATTCATCTCAAGTATCTGAATTTGTCTTTCAACACACTCCAAGGTGAAATTCCTACAGGTGGACCTTTTCAAAACCTCTCAGCTCAATCATTTGTCCCGAATGGTCCACTCTGTGGTGAATCCCGACTCCGTGTTCCACCCTGCAAAAATAGTACAGCCTCTCAACCACATTCAAGGAAAGCTAGTATATCCACCCTGAAATATATAATTCCAGGGATCATATCAGCAATACTCCTATTGGCCTTTATATCGATGTTGATACTACGGAGGAAAAGGGGGAATGCTATAGTTGCAACAAATACTGCGTTGGTACCTCTACTTCTCTGCAGAAGAGTTTCATACCTAGAACTTCTAAGGGGGACAAATGGATTTAGTGCAAACAACTTACTTGGTACAGGGGGTTTTAGCACAGTATATAGAGGGACACTTTCAGATGGGATAGATGTTGCAGTAAAGGTCTTCAATTTACAAGTAGAAGGGGGTTTCAAGAGTTTTGACAAGGAATGTGAAATGCTAAGCAATACACGTCATCGAAATCTTCTCAAAATCATTAGTTGCTGCTCCCAAATTGATTTCAAAGCCGTGGTACTGGAATACATGCCTAACGGGAGCTTAGAGAAGTGGTTGTACTCTTCAACATGTTCTTTGAGTGTCCTTGAGAGGTTGAATATAATGATACATGTTGCATCGGCATTGGAATACCTTCACCATGGCTATCAATTACCTATTGTTCATTGTGATTTGAAGCCAAGCAACATACTCCTAAATGATGACATGGTTGCACGTGTAGCTGATTTTGGGATCGCAAAACTTTTGGGAGGAGGAGATTCTGTGACTCAGACGTTGACCCTAGCCACAATTGGGTATATGGCTCCAGAGTACGGAGTGGAAGGAATTGTTTCGACAAGAGGGGATGTGTACAGTTTTGGTATTGTAATGATGGAAATATTCACGAAAAGGAAGCCAACGGATGAAATGTTTGCTGGGGAAATTAATTTAAAGGAATGGATTGCAAATTCATTACCAGAAGATGGAATAACAGAAGTTCTGGATGTCACTTTACTTGGTATAGAGGATGATGGTGATATATTTGTGGTCAAGAGAGATTGTTTGTCATCCCTTATGAGATTGGCCTTGGCGTGTTCTGCGGAGTCACCGCAAGAGAGGATAAACATACAAGATGTTGTTGTCACCCTCAATAAAATCAAGATAAAGCTTTTGAAGGCTGTTGCATGA

Protein Analysis

835

Amino Acids

90.93

Weight (kDa)

6.11

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 65 - 227 8.2e-06 Leucine-rich repeat region
LRR_8 PF13855 233 - 291 8.5e-07 Leucine rich repeat
LRR_14 PF23598 323 - 436 3e-07 Leucine-rich repeat region
LRR_8 PF13855 378 - 436 2.3e-08 Leucine rich repeat
Pkinase PF00069 549 - 818 3e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 550 - 821 3.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1579
AccB1I GGYRCC 1 cut(s) 1579
AccB7I CCANNNNNTGG 4 cut(s) 141, 1132, 1382, 2300
AccIII TCCGGA 1 cut(s) 1238
AciI CCGC 2 cut(s) 2417, 2426
AclWI GGATC 6 cut(s) 711, 724, 988, 1224, 1493, 2078
AcuI CTGAAG 1 cut(s) 673
AcvI CACGTG 1 cut(s) 2054
AdeI CACNNNGTG 1 cut(s) 1382
AfaI GTAC 9 cut(s) 196, 885, 1423, 1581, 1663, 1857, 1898, 2150, 2189
AfiI CCNNNNNNNGG 7 cut(s) 141, 1132, 1328, 1382, 2130, 2150, 2300
AflIII ACRYGT 4 cut(s) 1793, 1907, 1948, 2053
AjiI CACGTC 1 cut(s) 1796
AjnI CCWGG 4 cut(s) 771, 1125, 1203, 1480
AjuI GAANNNNNNNTTGG 2 cut(s) 1302, 1334
Alw26I GTCTC 4 cut(s) 298, 782, 1145, 1260
AlwI GGATC 6 cut(s) 711, 724, 988, 1224, 1493, 2078
Aor13HI TCCGGA 1 cut(s) 1238
AoxI GGCC 2 cut(s) 1509, 2400
ApeKI GCWGC 1 cut(s) 1827
AseI ATTAAT 1 cut(s) 2262
Asp700I GAANNNNTTC 3 cut(s) 1603, 1971, 2167
Asp718I GGTACC 1 cut(s) 1579
AspA2I CCTAGG 1 cut(s) 895
AspS9I GGNCC 5 cut(s) 122, 266, 1025, 1331, 1373
AsuHPI GGTGA 7 cut(s) 227, 1325, 1397, 1967, 2363, 2415, 2452
AsuII TTCGAA 1 cut(s) 544
AvaII GGWCC 5 cut(s) 122, 266, 1025, 1331, 1373
AvrII CCTAGG 1 cut(s) 895
BamHI GGATCC 1 cut(s) 716
BanI GGYRCC 1 cut(s) 1579
BarI GAAGNNNNNNTAC 4 cut(s) 1572, 1604, 1880, 1912
BauI CACGAG 1 cut(s) 451
BbrPI CACGTG 1 cut(s) 2054
BbsI GAAGAC 3 cut(s) 335, 763, 1720
BbvI GCAGC 1 cut(s) 1814
BccI CCATC 8 cut(s) 113, 139, 706, 970, 1697, 2203, 2294, 2342
BceAI ACGGC 1 cut(s) 1835
BciT130I CCWGG 4 cut(s) 773, 1127, 1205, 1482
BciVI GTATCC 1 cut(s) 800
BcoDI GTCTC 4 cut(s) 298, 782, 1145, 1260
BfaI CTAG 9 cut(s) 377, 458, 509, 842, 896, 998, 1452, 1613, 2120
BfmI CTRYAG 3 cut(s) 1323, 1554, 1594
BfuI GTATCC 1 cut(s) 800
BglII AGATCT 1 cut(s) 90
BisI GCNGC 1 cut(s) 1828
BlnI CCTAGG 1 cut(s) 895
BlpI GCTNAGC 2 cut(s) 1199, 1784
BlsI GCNGC 1 cut(s) 1829
Bme1390I CCNGG 4 cut(s) 773, 1127, 1205, 1482
Bme18I GGWCC 5 cut(s) 122, 266, 1025, 1331, 1373
BmgBI CACGTC 1 cut(s) 1796
BmgT120I GGNCC 5 cut(s) 122, 266, 1025, 1331, 1373
BmiI GGNNCC 4 cut(s) 718, 880, 1581, 2142
BmrFI CCNGG 4 cut(s) 773, 1127, 1205, 1482
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 1 cut(s) 1964
BmuI ACTGGG 1 cut(s) 1173
BpiI GAAGAC 3 cut(s) 335, 763, 1720
BpmI CTGGAG 3 cut(s) 755, 1109, 2127
Bpu10I CCTNAGC 1 cut(s) 469
Bpu1102I GCTNAGC 2 cut(s) 1199, 1784
Bpu14I TTCGAA 1 cut(s) 544
BpuEI CTTGAG 3 cut(s) 115, 1262, 1946
Bsa29I ATCGAT 1 cut(s) 1520
BsaAI YACGTR 1 cut(s) 2054
BsaI GGTCTC 1 cut(s) 782
BsaJI CCNNGG 9 cut(s) 604, 772, 895, 1146, 1309, 1481, 1851, 1978, 2403
BsaWI WCCGGW 1 cut(s) 1238
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 2085, 2115
Bsc4I CCNNNNNNNGG 7 cut(s) 141, 1132, 1328, 1382, 2130, 2150, 2300
Bse1I ACTGG 4 cut(s) 49, 837, 1179, 1863
Bse3DI GCAATG 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 1238
BseBI CCWGG 4 cut(s) 773, 1127, 1205, 1482
BseCI ATCGAT 1 cut(s) 1520
BseDI CCNNGG 9 cut(s) 604, 772, 895, 1146, 1309, 1481, 1851, 1978, 2403
BseGI GGATG 9 cut(s) 131, 519, 810, 1007, 2188, 2245, 2326, 2350, 2384
BseLI CCNNNNNNNGG 7 cut(s) 141, 1132, 1328, 1382, 2130, 2150, 2300
BseMI GCAATG 1 cut(s) 152
BseMII CTCAG 6 cut(s) 348, 421, 793, 1047, 1362, 2120
BseNI ACTGG 4 cut(s) 49, 837, 1179, 1863
BseRI GAGGAG 2 cut(s) 2103, 2106
BseXI GCAGC 1 cut(s) 1814
BseYI CCCAGC 1 cut(s) 2252
BshFI GGCC 2 cut(s) 1511, 2402
BshNI GGYRCC 1 cut(s) 1579
BshVI ATCGAT 1 cut(s) 1520
BsiSI CCGG 1 cut(s) 1239
BslFI GGGAC 4 cut(s) 570, 1349, 1642, 1705
BslI CCNNNNNNNGG 7 cut(s) 141, 1132, 1328, 1382, 2130, 2150, 2300
BsmAI GTCTC 4 cut(s) 298, 782, 1145, 1260
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 4 cut(s) 570, 1349, 1642, 1705
BsmI GAATGC 1 cut(s) 1555
BsnI GGCC 2 cut(s) 1511, 2402
Bso31I GGTCTC 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 544
Bsp13I TCCGGA 1 cut(s) 1238
Bsp1407I TGTACA 1 cut(s) 2187
Bsp143I GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
Bsp1720I GCTNAGC 2 cut(s) 1199, 1784
Bsp19I CCATGG 1 cut(s) 1978
BspACI CCGC 2 cut(s) 2417, 2426
BspANI GGCC 2 cut(s) 1511, 2402
BspCNI CTCAG 6 cut(s) 347, 420, 792, 1046, 1361, 2119
BspDI ATCGAT 1 cut(s) 1520
BspEI TCCGGA 1 cut(s) 1238
BspLI GGNNCC 4 cut(s) 718, 880, 1581, 2142
BspMAI CTGCAG 1 cut(s) 1598
BspPI GGATC 6 cut(s) 711, 724, 988, 1224, 1493, 2078
BspT104I TTCGAA 1 cut(s) 544
BspT107I GGYRCC 1 cut(s) 1579
BspTNI GGTCTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 2187
BsrI ACTGG 4 cut(s) 49, 837, 1179, 1863
BssECI CCNNGG 9 cut(s) 604, 772, 895, 1146, 1309, 1481, 1851, 1978, 2403
BssMI GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
BssSI CACGAG 1 cut(s) 451
BssT1I CCWWGG 6 cut(s) 604, 895, 1146, 1309, 1978, 2403
Bst2BI CACGAG 1 cut(s) 451
Bst2UI CCWGG 4 cut(s) 773, 1127, 1205, 1482
Bst4CI ACNGT 2 cut(s) 1681, 2192
Bst6I CTCTTC 3 cut(s) 597, 1594, 1906
BstAUI TGTACA 1 cut(s) 2187
BstBAI YACGTR 1 cut(s) 2054
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 106
BstDSI CCRYGG 2 cut(s) 1851, 1978
BstF5I GGATG 9 cut(s) 131, 519, 810, 1007, 2188, 2245, 2326, 2350, 2384
BstKTI GATC 7 cut(s) 93, 220, 719, 996, 1219, 1488, 2073
BstMAI GTCTC 4 cut(s) 298, 782, 1145, 1260
BstMBI GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
BstMWI GCNNNNNNNGC 5 cut(s) 163, 454, 1559, 2057, 2414
BstNI CCWGG 4 cut(s) 773, 1127, 1205, 1482
BstNSI RCATGY 3 cut(s) 1870, 1911, 1952
BstSCI CCNGG 4 cut(s) 771, 1125, 1203, 1480
BstSFI CTRYAG 3 cut(s) 1323, 1554, 1594
BstV1I GCAGC 1 cut(s) 1814
BstV2I GAAGAC 3 cut(s) 335, 763, 1720
BstX2I RGATCY 3 cut(s) 90, 716, 1216
BstXI CCANNNNNNTGG 1 cut(s) 1094
BstYI RGATCY 3 cut(s) 90, 716, 1216
Bsu15I ATCGAT 1 cut(s) 1520
BsuI GTATCC 1 cut(s) 800
BsuRI GGCC 2 cut(s) 1511, 2402
BsuTUI ATCGAT 1 cut(s) 1520
BtgI CCRYGG 2 cut(s) 1851, 1978
BtrI CACGTC 1 cut(s) 1796
BtsCI GGATG 9 cut(s) 131, 519, 810, 1007, 2188, 2245, 2326, 2350, 2384
BtsIMutI CAGTG 3 cut(s) 415, 697, 865
Cac8I GCNNGC 1 cut(s) 106
Cfr13I GGNCC 5 cut(s) 122, 266, 1025, 1331, 1373
ClaI ATCGAT 1 cut(s) 1520
Csp6I GTAC 9 cut(s) 195, 884, 1422, 1580, 1662, 1856, 1897, 2149, 2188
CspCI CAANNNNNGTGG 2 cut(s) 732, 767
CviAII CATG 9 cut(s) 298, 385, 788, 1867, 1908, 1949, 1979, 2044, 2502
CviQI GTAC 9 cut(s) 195, 884, 1422, 1580, 1662, 1856, 1897, 2149, 2188
DpnI GATC 7 cut(s) 92, 219, 718, 995, 1218, 1487, 2072
DpnII GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
DraI TTTAAA 1 cut(s) 2268
DraIII CACNNNGTG 1 cut(s) 1382
Eam1104I CTCTTC 3 cut(s) 597, 1594, 1906
EarI CTCTTC 3 cut(s) 597, 1594, 1906
Eco130I CCWWGG 6 cut(s) 604, 895, 1146, 1309, 1978, 2403
Eco31I GGTCTC 1 cut(s) 782
Eco32I GATATC 2 cut(s) 702, 927
Eco47I GGWCC 5 cut(s) 122, 266, 1025, 1331, 1373
Eco57I CTGAAG 1 cut(s) 673
Eco72I CACGTG 1 cut(s) 2054
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 4 cut(s) 771, 1125, 1203, 1480
EcoRV GATATC 2 cut(s) 702, 927
EcoT14I CCWWGG 6 cut(s) 604, 895, 1146, 1309, 1978, 2403
ErhI CCWWGG 6 cut(s) 604, 895, 1146, 1309, 1978, 2403
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 9 cut(s) 301, 388, 791, 1870, 1911, 1952, 1982, 2047, 2505
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 4 cut(s) 570, 1349, 1642, 1705
FatI CATG 9 cut(s) 297, 384, 787, 1866, 1907, 1948, 1978, 2043, 2501
Fnu4HI GCNGC 1 cut(s) 1828
FokI GGATG 9 cut(s) 118, 506, 797, 994, 2195, 2252, 2333, 2357, 2371
Fsp4HI GCNGC 1 cut(s) 1828
FspBI CTAG 9 cut(s) 377, 458, 509, 842, 896, 998, 1452, 1613, 2120
GluI GCNGC 1 cut(s) 1828
GsaI CCCAGC 1 cut(s) 2256
GsuI CTGGAG 3 cut(s) 755, 1109, 2127
HaeIII GGCC 2 cut(s) 1511, 2402
HapII CCGG 1 cut(s) 1239
Hin1II CATG 9 cut(s) 301, 388, 791, 1870, 1911, 1952, 1982, 2047, 2505
HincII GTYRAC 4 cut(s) 34, 71, 948, 2115
HindII GTYRAC 4 cut(s) 34, 71, 948, 2115
HindIII AAGCTT 2 cut(s) 977, 2483
HpaI GTTAAC 2 cut(s) 34, 948
HpaII CCGG 1 cut(s) 1239
HphI GGTGA 7 cut(s) 227, 1325, 1397, 1967, 2363, 2415, 2452
Hpy188I TCNGA 5 cut(s) 90, 1036, 1287, 1702, 2109
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 6 cut(s) 897, 1160, 1739, 1982, 2153, 2485
HpyCH4III ACNGT 2 cut(s) 1681, 2192
HpyCH4IV ACGT 4 cut(s) 620, 1795, 2053, 2111
HpyF10VI GCNNNNNNNGC 5 cut(s) 163, 454, 1559, 2057, 2414
HpySE526I ACGT 4 cut(s) 620, 1795, 2053, 2111
Hsp92II CATG 9 cut(s) 301, 388, 791, 1870, 1911, 1952, 1982, 2047, 2505
Kpn2I TCCGGA 1 cut(s) 1238
KpnI GGTACC 1 cut(s) 1583
KspAI GTTAAC 2 cut(s) 34, 948
Kzo9I GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
LmnI GCTCC 3 cut(s) 1835, 1878, 2146
Lsp1109I GCAGC 1 cut(s) 1814
LweI GCATC 1 cut(s) 1964
MaeI CTAG 9 cut(s) 377, 458, 509, 842, 896, 998, 1452, 1613, 2120
MaeII ACGT 4 cut(s) 620, 1795, 2053, 2111
MaeIII GTNAC 5 cut(s) 1060, 2101, 2323, 2421, 2458
MalI GATC 7 cut(s) 92, 219, 718, 995, 1218, 1487, 2072
MboI GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
MfeI CAATTG 2 cut(s) 725, 2127
MflI RGATCY 3 cut(s) 90, 716, 1216
MlyI GAGTC 6 cut(s) 552, 944, 1116, 1389, 2098, 2429
MmeI TCCRAC 4 cut(s) 371, 373, 767, 1024
MroI TCCGGA 1 cut(s) 1238
MroXI GAANNNNTTC 3 cut(s) 1603, 1971, 2167
MslI CAYNNNNRTG 1 cut(s) 1092
MspI CCGG 1 cut(s) 1239
MspR9I CCNGG 4 cut(s) 773, 1127, 1205, 1482
MunI CAATTG 2 cut(s) 725, 2127
Mva1269I GAATGC 1 cut(s) 1555
MvaI CCWGG 4 cut(s) 773, 1127, 1205, 1482
MwoI GCNNNNNNNGC 5 cut(s) 163, 454, 1559, 2057, 2414
NcoI CCATGG 1 cut(s) 1978
NdeII GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
NlaIII CATG 9 cut(s) 301, 388, 791, 1870, 1911, 1952, 1982, 2047, 2505
NlaIV GGNNCC 4 cut(s) 718, 880, 1581, 2142
NmuCI GTSAC 5 cut(s) 1060, 2101, 2323, 2421, 2458
NspI RCATGY 3 cut(s) 1870, 1911, 1952
NspV TTCGAA 1 cut(s) 544
PciI ACATGT 2 cut(s) 1907, 1948
PctI GAATGC 1 cut(s) 1555
PdmI GAANNNNTTC 3 cut(s) 1603, 1971, 2167
PfeI GAWTC 4 cut(s) 417, 766, 1388, 2096
PflMI CCANNNNNTGG 4 cut(s) 141, 1132, 1382, 2300
PfoI TCCNGGA 1 cut(s) 1125
PkrI GCNGC 1 cut(s) 1829
PleI GAGTC 6 cut(s) 552, 943, 1116, 1389, 2098, 2428
PmaCI CACGTG 1 cut(s) 2054
PmlI CACGTG 1 cut(s) 2054
PpsI GAGTC 6 cut(s) 552, 943, 1116, 1389, 2098, 2428
Ppu21I YACGTR 1 cut(s) 2054
PscI ACATGT 2 cut(s) 1907, 1948
PshBI ATTAAT 1 cut(s) 2262
Psp6I CCWGG 4 cut(s) 771, 1125, 1203, 1480
PspCI CACGTG 1 cut(s) 2054
PspFI CCCAGC 1 cut(s) 2252
PspGI CCWGG 4 cut(s) 771, 1125, 1203, 1480
PspN4I GGNNCC 4 cut(s) 718, 880, 1581, 2142
PspPI GGNCC 5 cut(s) 122, 266, 1025, 1331, 1373
PstI CTGCAG 1 cut(s) 1598
PsuI RGATCY 3 cut(s) 90, 716, 1216
RsaI GTAC 9 cut(s) 196, 885, 1423, 1581, 1663, 1857, 1898, 2150, 2189
RsaNI GTAC 9 cut(s) 195, 884, 1422, 1580, 1662, 1856, 1897, 2149, 2188
RseI CAYNNNNRTG 1 cut(s) 1092
SatI GCNGC 1 cut(s) 1828
Sau3AI GATC 7 cut(s) 90, 217, 716, 993, 1216, 1485, 2070
Sau96I GGNCC 5 cut(s) 122, 266, 1025, 1331, 1373
SchI GAGTC 6 cut(s) 552, 944, 1116, 1389, 2098, 2429
ScrFI CCNGG 4 cut(s) 773, 1127, 1205, 1482
SfaNI GCATC 1 cut(s) 1964
SfcI CTRYAG 3 cut(s) 1323, 1554, 1594
SfuI TTCGAA 1 cut(s) 544
SinI GGWCC 5 cut(s) 122, 266, 1025, 1331, 1373
SmiMI CAYNNNNRTG 1 cut(s) 1092
SmlI CTYRAG 3 cut(s) 94, 1277, 1925
SmoI CTYRAG 3 cut(s) 94, 1277, 1925
SsiI CCGC 2 cut(s) 2417, 2426
SspI AATATT 1 cut(s) 2217
SspMI CTAG 9 cut(s) 377, 458, 509, 842, 896, 998, 1452, 1613, 2120
StyD4I CCNGG 4 cut(s) 771, 1125, 1203, 1480
StyI CCWWGG 6 cut(s) 604, 895, 1146, 1309, 1978, 2403
TaaI ACNGT 2 cut(s) 1681, 2192
TaiI ACGT 4 cut(s) 623, 1798, 2056, 2114
TaqI TCGA 5 cut(s) 246, 544, 1520, 1801, 2171
TatI WGTACW 3 cut(s) 1421, 1896, 2187
TfiI GAWTC 4 cut(s) 417, 766, 1388, 2096
TscAI CASTG 3 cut(s) 415, 697, 865
TseFI GTSAC 5 cut(s) 1060, 2101, 2323, 2421, 2458
TseI GCWGC 1 cut(s) 1827
Tsp45I GTSAC 5 cut(s) 1060, 2101, 2323, 2421, 2458
TspGWI ACGGA 5 cut(s) 871, 1388, 1549, 2166, 2252
TspRI CASTG 3 cut(s) 415, 697, 865
Van91I CCANNNNNTGG 4 cut(s) 141, 1132, 1382, 2300
VpaK11BI GGWCC 5 cut(s) 122, 266, 1025, 1331, 1373
VspI ATTAAT 1 cut(s) 2262
XceI RCATGY 3 cut(s) 1870, 1911, 1952
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 969
XmaJI CCTAGG 1 cut(s) 895
XmnI GAANNNNTTC 3 cut(s) 1603, 1971, 2167
XspI CTAG 9 cut(s) 377, 458, 509, 842, 896, 998, 1452, 1613, 2120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.