MD07G1168700.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000058
Physical Location & Seq
Reverse (-)
24349897 .. 24352596
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1168700.v1.1.491

Sequence Viewer

Length: 2397 bp
ATGTCCAACTTGAAGTACTTCAGTATGGAATTGAATAACATCAAGGGAAGTATTCCTAGTGGCGTAGGGCGTCTTCCTAGTTTAGTCTCTGTTTTTTTCGCCGGTAATGGGCTGAGCGGAGTTTTACCCCCAGGAATTTTCAATATCTCCACCATAACAGATATCGCCGGAAGAGATAATGCCCTTACCGGAAGTTTCCCTTCATATACTGGAGGGATTTGGGGTCCTAACCTTGAAACCCTCGGGCTTTCAACCAATCAAATCACTGGAAATATCCCGTCCTACTTTTCAAATTTTAGTAAGCTTACCATGCTTGATTGTAGCTACAACTTACTCCATGGACCAGTTCCCATGGATCTTGGTAACTTAAAACACCTTAACTTTCTTGTTTTAGGAGCAAATAAGTTAACAGGAGAACCTGGAGTTCTTGAGCTTAGGTTCCTTTCTTCCTTATTCAGTAGCAGCTCGTTGAAAACATTAGTTGTGAGTGAAAATCCCCTCAACGGCATCATCCCGGATCACTCCGGAAACTTTTCTTCTTCCTCTTCCCTTCGAAAGTTGTATGCACAGCTGTGCCAAATCAAGGGTCATATACCCAAGACGTTGAGCTCCTTGTTGAAAAATTTGACCATTCTTGCCTTGACTGGCAACAGTATCAGTGGAGAAATACCTCCATCGATTGGAAGATTCGAGAATCTGCAAATCTTGTATCTCGATAACAACAGCATTGAAGGTTTCATTCCGGAGGAGTTATGTCGTATCGGGAACCTGGCTGAGCTATCACTGGCAAGCAATACCATTGGTGGATTGATCCCAAGTTGCATAGGAAACCTCAGATACCTTCAAAGAGTTTTCCTAAATTCCAACAAACTGAACTCATCAATACCAGTCAATTTATGGAACCTTGAAGACCTCCTGTTCCTAGATTTGTCATTCAATTCCTTAAGTGGATACTTACCTCCTGACCTGAGAATGTCAAATGTCCTTGAGATGATAGATTTGTCTTGGAACCGAATTTCTGGAGACATTCCGAGCATCATGGGATCCTTTCAAAGCATAAACTCTCTAAATTTGTCCAACAACTTGTTCATCGGATCTATTCCACATACTTTTGGGAAAGGGTTGGAAATTTTAGACCTGTCATGCAATAATCTGTCCGGGCCAATACCAAAGTCTCTAGAAATACTCAAATTTCTCAAGTATTTGAATCTCTCTTTTAATCAACTCTCTGGAGAGATTCCATCAGCTGGACCTTTTGTTAACTTCACTGCCCAATCATTTTTGGGGAATAAAGCACTCTGTGGGAGACCAGGTTTTGGAGTCCAACCTTGCAAGAATCTTAGCACTCGAAATTCAAAGACCGCGCATTATTTCCTCAGATATGCTATTCCGGCTGTGATATCAACTTTGATTCTGATAGCCGCCATTTTTATGTGGAGAAAGTCTAGAAAAACAAGTGCAAGTGTTCCAAGTTCAGAAGAATTGTCAATGGCTCCAGGGCATAGAATGATATCATATCATGAACTCCGTTGTGCTACAAATGATTTCTCTGAAAGCAACTTTCTGGGAGCCGGAAGTTTTGGCTCTGTGTACAAGGGAAGTCTTTCCGATGGGACAACCGTTGCTGTCAAAATTCTAAATATGCAAATGGAAGGTGCCTCGAAAAGTTTCATTGCAGAATGCAAAGTATGGCGAGCAATACGACATAGAAATCTTGTCAAGGTGATAACTACGTGCTCGAGTCCAGAGGTAAGAGCTTTGGTGCTGCAATACATGTCTAATGGTAGCCTCGAGGAGTGGTTGTACTCTCGTAATTATTGTCTAAATCTTTTTCAAAGAGTGAGCCTGTTGGTCGATGTTGCATCAGCTTTGGAATATCTCCATCAAGGCCAATTAGAGCCTGTAGTGCACTGTGATTTGAAGCCTAGCAACATCCTTCTTGACGAAGACATGGTTGCACATGTGGGGGACTTTGGTCTTGCTAAAATTTTGGCAGGAAACAAGGATGCAACACAAACCAGAACTCTTGGTACACTTGGTTATGTTGTACCAGAGTATGGTTCAGCAGGAAAAGTGTCAACAAGAGGTGATGTGTATAGCTATGGAATCATGTTGTTTGAAATAATTGCAAGAAAGAGGCCAACAGATGGAATGTTTACGGAAGAACTGACCCTGAGGAAATGGATAAATGCGTCGCTCCCTGACGGTATTCTAGGAGTAGTGGATATTGGTTTATTGAGCACAGAAGAGGGAAGAGAGATGAATGCCACAAAACGTATCATTTTATCAATCATGGACATCGGCTTGAGATGTTCCGAAGAAGTGCCAGAAGAAAGAATGGATATCAAAGATGTGTTGCGGAAGCTCATAAAAATAAAATCGGCACTTGAAATGTAG

Protein Analysis

799

Amino Acids

87.24

Weight (kDa)

7.86

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 76 - 136 1.4e-06 Leucine rich repeat
LRR_14 PF23598 178 - 314 3.7e-09 Leucine-rich repeat region
LRR_8 PF13855 208 - 267 1.5e-06 Leucine rich repeat
LRR_8 PF13855 256 - 315 4.7e-06 Leucine rich repeat
Pkinase PF00069 518 - 722 3.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 520 - 788 3.2e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1790
AccB1I GGYRCC 1 cut(s) 1655
AccB7I CCANNNNNTGG 5 cut(s) 680, 1247, 1316, 2057, 2147
AccBSI CCGCTC 1 cut(s) 117
AccII CGCG 1 cut(s) 1364
AccIII TCCGGA 2 cut(s) 524, 742
AciI CCGC 4 cut(s) 117, 1362, 1422, 2359
AclWI GGATC 6 cut(s) 363, 525, 805, 1038, 1051, 1102
AcuI CTGAAG 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 70
AdeI CACNNNGTG 1 cut(s) 1301
AfaI GTAC 5 cut(s) 17, 1592, 1805, 2032, 2049
AfiI CCNNNNNNNGG 8 cut(s) 108, 503, 583, 680, 1247, 1316, 2057, 2147
AflII CTTAAG 1 cut(s) 943
AflIII ACRYGT 2 cut(s) 1773, 1960
AjnI CCWGG 5 cut(s) 130, 418, 768, 1309, 1495
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AleI CACNNNNGTG 1 cut(s) 571
Alw21I GWGCWC 4 cut(s) 611, 1739, 1911, 2243
Alw26I GTCTC 4 cut(s) 91, 1017, 1179, 1300
Alw44I GTGCAC 1 cut(s) 1907
AlwI GGATC 6 cut(s) 363, 525, 805, 1038, 1051, 1102
Ama87I CYCGRG 3 cut(s) 242, 1738, 1790
Aor13HI TCCGGA 2 cut(s) 524, 742
AoxI GGCC 3 cut(s) 1160, 1888, 2138
ApaLI GTGCAC 1 cut(s) 1907
ApeKI GCWGC 2 cut(s) 462, 1765
ArsI GACNNNNNNTTYG 2 cut(s) 571, 603
Asp700I GAANNNNTTC 4 cut(s) 17, 532, 1603, 1667
AspLEI GCGC 1 cut(s) 1366
AspS9I GGNCC 4 cut(s) 224, 341, 1160, 1250
AsuC2I CCSGG 2 cut(s) 515, 1159
AsuHPI GGTGA 2 cut(s) 1735, 2099
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 3 cut(s) 242, 1738, 1790
AvaII GGWCC 3 cut(s) 224, 341, 1250
AxyI CCTNAGG 1 cut(s) 2174
BaeGI GKGCMC 1 cut(s) 1911
BamHI GGATCC 1 cut(s) 1043
BanI GGYRCC 1 cut(s) 1655
BanII GRGCYC 1 cut(s) 611
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BbsI GAAGAC 3 cut(s) 65, 915, 1953
Bbv12I GWGCWC 4 cut(s) 611, 1739, 1911, 2243
BbvI GCAGC 2 cut(s) 474, 1752
BccI CCATC 5 cut(s) 682, 1249, 1604, 1890, 2141
BceAI ACGGC 1 cut(s) 520
BciT130I CCWGG 5 cut(s) 132, 420, 770, 1311, 1497
BciVI GTATCC 1 cut(s) 944
BcnI CCSGG 2 cut(s) 515, 1159
BcoDI GTCTC 4 cut(s) 91, 1017, 1179, 1300
BfaI CTAG 7 cut(s) 57, 78, 923, 1178, 1446, 1926, 2213
BfmI CTRYAG 1 cut(s) 1902
BfrI CTTAAG 1 cut(s) 943
BfuI GTATCC 1 cut(s) 944
BisI GCNGC 3 cut(s) 463, 1422, 1766
BlpI GCTNAGC 2 cut(s) 113, 774
BlsI GCNGC 3 cut(s) 464, 1423, 1767
BmcAI AGTACT 1 cut(s) 17
Bme1390I CCNGG 7 cut(s) 132, 420, 515, 770, 1159, 1311, 1497
Bme18I GGWCC 3 cut(s) 224, 341, 1250
BmeT110I CYCGRG 3 cut(s) 242, 1738, 1790
BmgT120I GGNCC 4 cut(s) 224, 341, 1160, 1250
BmiI GGNNCC 9 cut(s) 225, 440, 767, 902, 1010, 1045, 1494, 1570, 1657
BmrFI CCNGG 7 cut(s) 132, 420, 515, 770, 1159, 1311, 1497
BmsI GCATC 4 cut(s) 516, 1044, 1871, 1996
BoxI GACNNNNGTC 1 cut(s) 1974
BpiI GAAGAC 3 cut(s) 65, 915, 1953
BpmI CTGGAG 5 cut(s) 231, 441, 1041, 1251, 1479
Bpu10I CCTNAGC 1 cut(s) 434
Bpu1102I GCTNAGC 2 cut(s) 113, 774
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 4 cut(s) 449, 1007, 1181, 2326
BpuMI CCSGG 2 cut(s) 515, 1159
Bsa29I ATCGAT 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 1734
BsaHI GRCGYC 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 1300
BsaJI CCNNGG 5 cut(s) 130, 241, 337, 351, 1496
BsaWI WCCGGW 3 cut(s) 188, 524, 742
BsaXI ACNNNNNCTCC 4 cut(s) 1297, 1327, 1787, 1817
Bsc4I CCNNNNNNNGG 8 cut(s) 108, 503, 583, 680, 1247, 1316, 2057, 2147
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 6 cut(s) 214, 271, 344, 649, 789, 887
Bse21I CCTNAGG 1 cut(s) 2174
Bse3DI GCAATG 1 cut(s) 1671
BseAI TCCGGA 2 cut(s) 524, 742
BseBI CCWGG 5 cut(s) 132, 420, 770, 1311, 1497
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 5 cut(s) 130, 241, 337, 351, 1496
BseGI GGATG 3 cut(s) 510, 1932, 2011
BseLI CCNNNNNNNGG 8 cut(s) 108, 503, 583, 680, 1247, 1316, 2057, 2147
BseMI GCAATG 1 cut(s) 1671
BseMII CTCAG 6 cut(s) 104, 765, 847, 959, 1390, 2165
BseNI ACTGG 6 cut(s) 214, 271, 344, 649, 789, 887
BseRI GAGGAG 2 cut(s) 761, 1808
BseSI GKGCMC 1 cut(s) 1911
BseXI GCAGC 2 cut(s) 474, 1752
Bsh1236I CGCG 1 cut(s) 1364
BshFI GGCC 3 cut(s) 1162, 1890, 2140
BshNI GGYRCC 1 cut(s) 1655
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 4 cut(s) 611, 1739, 1911, 2243
BsiHKCI CYCGRG 3 cut(s) 242, 1738, 1790
BsiSI CCGG 9 cut(s) 102, 168, 189, 515, 525, 743, 1158, 1391, 1572
BslFI GGGAC 2 cut(s) 1627, 1982
BslI CCNNNNNNNGG 8 cut(s) 108, 503, 583, 680, 1247, 1316, 2057, 2147
BsmAI GTCTC 4 cut(s) 91, 1017, 1179, 1300
BsmFI GGGAC 2 cut(s) 1627, 1982
BsmI GAATGC 2 cut(s) 1685, 2269
BsnI GGCC 3 cut(s) 1162, 1890, 2140
Bso31I GGTCTC 1 cut(s) 1300
BsoBI CYCGRG 3 cut(s) 242, 1738, 1790
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 4 cut(s) 611, 1739, 1911, 2243
Bsp13I TCCGGA 2 cut(s) 524, 742
Bsp1407I TGTACA 1 cut(s) 1590
Bsp143I GATC 5 cut(s) 355, 517, 810, 1043, 1094
Bsp1720I GCTNAGC 2 cut(s) 113, 774
Bsp19I CCATGG 2 cut(s) 337, 351
BspACI CCGC 4 cut(s) 117, 1362, 1422, 2359
BspANI GGCC 3 cut(s) 1162, 1890, 2140
BspCNI CTCAG 6 cut(s) 105, 766, 846, 960, 1389, 2166
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 2 cut(s) 524, 742
BspFNI CGCG 1 cut(s) 1364
BspHI TCATGA 1 cut(s) 1519
BspLI GGNNCC 9 cut(s) 225, 440, 767, 902, 1010, 1045, 1494, 1570, 1657
BspPI GGATC 6 cut(s) 363, 525, 805, 1038, 1051, 1102
BspT104I TTCGAA 1 cut(s) 553
BspT107I GGYRCC 1 cut(s) 1655
BspTI CTTAAG 1 cut(s) 943
BspTNI GGTCTC 1 cut(s) 1300
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 1671
BsrFI RCCGGY 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 1590
BsrI ACTGG 6 cut(s) 214, 271, 344, 649, 789, 887
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 5 cut(s) 130, 241, 337, 351, 1496
BssMI GATC 5 cut(s) 355, 517, 810, 1043, 1094
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 2 cut(s) 337, 351
Bst2UI CCWGG 5 cut(s) 132, 420, 770, 1311, 1497
Bst4CI ACNGT 4 cut(s) 653, 1621, 1913, 2207
Bst6I CTCTTC 4 cut(s) 166, 550, 2241, 2248
BstACI GRCGYC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 943
BstAUI TGTACA 1 cut(s) 1590
BstBAI YACGTR 1 cut(s) 1734
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 790, 1695
BstDEI CTNAG 8 cut(s) 113, 434, 774, 833, 968, 1340, 1376, 2174
BstDSI CCRYGG 2 cut(s) 337, 351
BstF5I GGATG 3 cut(s) 510, 1932, 2011
BstFNI CGCG 1 cut(s) 1364
BstHHI GCGC 1 cut(s) 1366
BstKTI GATC 5 cut(s) 358, 520, 813, 1046, 1097
BstMAI GTCTC 4 cut(s) 91, 1017, 1179, 1300
BstMBI GATC 5 cut(s) 355, 517, 810, 1043, 1094
BstMWI GCNNNNNNNGC 3 cut(s) 310, 1391, 1906
BstNI CCWGG 5 cut(s) 132, 420, 770, 1311, 1497
BstNSI RCATGY 2 cut(s) 1777, 1964
BstPAI GACNNNNGTC 1 cut(s) 1974
BstSCI CCNGG 7 cut(s) 130, 418, 513, 768, 1157, 1309, 1495
BstSFI CTRYAG 1 cut(s) 1902
BstSLI GKGCMC 1 cut(s) 1911
BstUI CGCG 1 cut(s) 1364
BstV1I GCAGC 2 cut(s) 474, 1752
BstV2I GAAGAC 3 cut(s) 65, 915, 1953
BstX2I RGATCY 3 cut(s) 355, 1043, 1094
BstYI RGATCY 3 cut(s) 355, 1043, 1094
Bsu15I ATCGAT 1 cut(s) 677
Bsu36I CCTNAGG 1 cut(s) 2174
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 3 cut(s) 1162, 1890, 2140
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 2 cut(s) 337, 351
BtsCI GGATG 3 cut(s) 510, 1932, 2011
BtsI GCAGTG 1 cut(s) 1266
BtsIMutI CAGTG 5 cut(s) 264, 664, 782, 1266, 1909
Cac8I GCNNGC 2 cut(s) 790, 1695
CciI TCATGA 1 cut(s) 1519
CfoI GCGC 1 cut(s) 1366
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 4 cut(s) 224, 341, 1160, 1250
ClaI ATCGAT 1 cut(s) 677
CseI GACGC 2 cut(s) 59, 2181
CsiI ACCWGGT 1 cut(s) 1309
Csp6I GTAC 5 cut(s) 16, 1591, 1804, 2031, 2048
CviQI GTAC 5 cut(s) 16, 1591, 1804, 2031, 2048
DdeI CTNAG 8 cut(s) 113, 434, 774, 833, 968, 1340, 1376, 2174
DpnI GATC 5 cut(s) 357, 519, 812, 1045, 1096
DpnII GATC 5 cut(s) 355, 517, 810, 1043, 1094
DraIII CACNNNGTG 1 cut(s) 1301
Eam1104I CTCTTC 4 cut(s) 166, 550, 2241, 2248
EarI CTCTTC 4 cut(s) 166, 550, 2241, 2248
Ecl136II GAGCTC 1 cut(s) 609
Eco130I CCWWGG 2 cut(s) 337, 351
Eco24I GRGCYC 1 cut(s) 611
Eco31I GGTCTC 1 cut(s) 1300
Eco32I GATATC 4 cut(s) 163, 1401, 1512, 2344
Eco47I GGWCC 3 cut(s) 224, 341, 1250
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 4
Eco81I CCTNAGG 1 cut(s) 2174
Eco88I CYCGRG 3 cut(s) 242, 1738, 1790
EcoICRI GAGCTC 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 5 cut(s) 130, 418, 768, 1309, 1495
EcoRV GATATC 4 cut(s) 163, 1401, 1512, 2344
EcoT14I CCWWGG 2 cut(s) 337, 351
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 2 cut(s) 337, 351
FalI AAGNNNNNCTT 2 cut(s) 184, 216
FaqI GGGAC 2 cut(s) 1627, 1982
Fnu4HI GCNGC 3 cut(s) 463, 1422, 1766
FokI GGATG 3 cut(s) 497, 1919, 2018
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 463, 1422, 1766
FspBI CTAG 7 cut(s) 57, 78, 923, 1178, 1446, 1926, 2213
GlaI GCGC 1 cut(s) 1365
GluI GCNGC 3 cut(s) 463, 1422, 1766
GsuI CTGGAG 5 cut(s) 231, 441, 1041, 1251, 1479
HaeIII GGCC 3 cut(s) 1162, 1890, 2140
HapII CCGG 9 cut(s) 102, 168, 189, 515, 525, 743, 1158, 1391, 1572
HgaI GACGC 2 cut(s) 59, 2181
HhaI GCGC 1 cut(s) 1366
Hin1I GRCGYC 1 cut(s) 70
Hin6I GCGC 1 cut(s) 1364
HinP1I GCGC 1 cut(s) 1364
HincII GTYRAC 3 cut(s) 408, 1261, 2079
HindII GTYRAC 3 cut(s) 408, 1261, 2079
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 9 cut(s) 687, 694, 1207, 1237, 1320, 1336, 1411, 1741, 2106
HpaI GTTAAC 2 cut(s) 408, 1261
HpaII CCGG 9 cut(s) 102, 168, 189, 515, 525, 743, 1158, 1391, 1572
HphI GGTGA 2 cut(s) 1735, 2099
Hpy166II GTNNAC 7 cut(s) 408, 1261, 1591, 1909, 2033, 2079, 2157
Hpy188I TCNGA 9 cut(s) 836, 1032, 1094, 1379, 1416, 1477, 1552, 1609, 2317
Hpy8I GTNNAC 7 cut(s) 408, 1261, 1591, 1909, 2033, 2079, 2157
Hpy99I CGWCG 1 cut(s) 2197
HpyAV CCTTC 6 cut(s) 210, 560, 725, 851, 1646, 1946
HpyCH4III ACNGT 4 cut(s) 653, 1621, 1913, 2207
HpyCH4IV ACGT 3 cut(s) 602, 1733, 2275
HpyF10VI GCNNNNNNNGC 3 cut(s) 310, 1391, 1906
HpyF3I CTNAG 8 cut(s) 113, 434, 774, 833, 968, 1340, 1376, 2174
HpySE526I ACGT 3 cut(s) 602, 1733, 2275
Hsp92I GRCGYC 1 cut(s) 70
HspAI GCGC 1 cut(s) 1364
Kpn2I TCCGGA 2 cut(s) 524, 742
KspAI GTTAAC 2 cut(s) 408, 1261
Kzo9I GATC 5 cut(s) 355, 517, 810, 1043, 1094
LmnI GCTCC 5 cut(s) 395, 614, 1498, 1568, 2202
Lsp1109I GCAGC 2 cut(s) 474, 1752
LweI GCATC 4 cut(s) 516, 1044, 1871, 1996
MabI ACCWGGT 1 cut(s) 1309
MaeI CTAG 7 cut(s) 57, 78, 923, 1178, 1446, 1926, 2213
MaeII ACGT 3 cut(s) 602, 1733, 2275
MaeIII GTNAC 1 cut(s) 362
MalI GATC 5 cut(s) 357, 519, 812, 1045, 1096
MbiI CCGCTC 1 cut(s) 117
MboI GATC 5 cut(s) 355, 517, 810, 1043, 1094
MflI RGATCY 3 cut(s) 355, 1043, 1094
MhlI GDGCHC 4 cut(s) 611, 1739, 1911, 2243
MlyI GAGTC 2 cut(s) 1329, 1750
MmeI TCCRAC 5 cut(s) 30, 888, 1101, 1104, 1348
MroI TCCGGA 2 cut(s) 524, 742
MroXI GAANNNNTTC 4 cut(s) 17, 532, 1603, 1667
MseI TTAA 6 cut(s) 368, 378, 407, 944, 1218, 1260
MslI CAYNNNNRTG 2 cut(s) 571, 1430
MspA1I CMGCKG 2 cut(s) 571, 1247
MspCI CTTAAG 1 cut(s) 943
MspI CCGG 9 cut(s) 102, 168, 189, 515, 525, 743, 1158, 1391, 1572
MspR9I CCNGG 7 cut(s) 132, 420, 515, 770, 1159, 1311, 1497
Mva1269I GAATGC 2 cut(s) 1685, 2269
MvaI CCWGG 5 cut(s) 132, 420, 770, 1311, 1497
MvnI CGCG 1 cut(s) 1364
MwoI GCNNNNNNNGC 3 cut(s) 310, 1391, 1906
NciI CCSGG 2 cut(s) 515, 1159
NcoI CCATGG 2 cut(s) 337, 351
NdeII GATC 5 cut(s) 355, 517, 810, 1043, 1094
NlaIV GGNNCC 9 cut(s) 225, 440, 767, 902, 1010, 1045, 1494, 1570, 1657
NspI RCATGY 2 cut(s) 1777, 1964
NspV TTCGAA 1 cut(s) 553
OliI CACNNNNGTG 1 cut(s) 571
PaeR7I CTCGAG 2 cut(s) 1738, 1790
PagI TCATGA 1 cut(s) 1519
PciI ACATGT 2 cut(s) 1773, 1960
PctI GAATGC 2 cut(s) 1685, 2269
PdmI GAANNNNTTC 4 cut(s) 17, 532, 1603, 1667
PfeI GAWTC 7 cut(s) 687, 694, 1207, 1237, 1336, 1411, 2106
PflMI CCANNNNNTGG 5 cut(s) 680, 1247, 1316, 2057, 2147
PfoI TCCNGGA 1 cut(s) 513
PkrI GCNGC 3 cut(s) 464, 1423, 1767
PleI GAGTC 2 cut(s) 1328, 1749
PpsI GAGTC 2 cut(s) 1328, 1749
Ppu21I YACGTR 1 cut(s) 1734
PpuMI RGGWCCY 1 cut(s) 224
PscI ACATGT 2 cut(s) 1773, 1960
PshAI GACNNNNGTC 1 cut(s) 1974
Psp124BI GAGCTC 1 cut(s) 611
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 5 cut(s) 130, 418, 768, 1309, 1495
PspGI CCWGG 5 cut(s) 130, 418, 768, 1309, 1495
PspN4I GGNNCC 9 cut(s) 225, 440, 767, 902, 1010, 1045, 1494, 1570, 1657
PspPI GGNCC 4 cut(s) 224, 341, 1160, 1250
PspPPI RGGWCCY 1 cut(s) 224
PspXI VCTCGAGB 2 cut(s) 1738, 1790
PsrI GAACNNNNNNTAC 2 cut(s) 2014, 2046
PsuI RGATCY 3 cut(s) 355, 1043, 1094
PvuII CAGCTG 2 cut(s) 571, 1247
RsaI GTAC 5 cut(s) 17, 1592, 1805, 2032, 2049
RsaNI GTAC 5 cut(s) 16, 1591, 1804, 2031, 2048
RseI CAYNNNNRTG 2 cut(s) 571, 1430
SacI GAGCTC 1 cut(s) 611
SaqAI TTAA 6 cut(s) 368, 378, 407, 944, 1218, 1260
SatI GCNGC 3 cut(s) 463, 1422, 1766
Sau3AI GATC 5 cut(s) 355, 517, 810, 1043, 1094
Sau96I GGNCC 4 cut(s) 224, 341, 1160, 1250
ScaI AGTACT 1 cut(s) 17
SchI GAGTC 2 cut(s) 1329, 1750
ScrFI CCNGG 7 cut(s) 132, 420, 515, 770, 1159, 1311, 1497
SduI GDGCHC 4 cut(s) 611, 1739, 1911, 2243
SexAI ACCWGGT 1 cut(s) 1309
SfaNI GCATC 4 cut(s) 516, 1044, 1871, 1996
SfcI CTRYAG 1 cut(s) 1902
Sfr274I CTCGAG 2 cut(s) 1738, 1790
SfuI TTCGAA 1 cut(s) 553
SinI GGWCC 3 cut(s) 224, 341, 1250
SlaI CTCGAG 2 cut(s) 1738, 1790
SmiMI CAYNNNNRTG 2 cut(s) 571, 1430
SmlI CTYRAG 7 cut(s) 428, 943, 986, 1196, 1738, 1790, 2305
SmoI CTYRAG 7 cut(s) 428, 943, 986, 1196, 1738, 1790, 2305
SsiI CCGC 4 cut(s) 117, 1362, 1422, 2359
SspMI CTAG 7 cut(s) 57, 78, 923, 1178, 1446, 1926, 2213
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 7 cut(s) 130, 418, 513, 768, 1157, 1309, 1495
StyI CCWWGG 2 cut(s) 337, 351
TaaI ACNGT 4 cut(s) 653, 1621, 1913, 2207
TaiI ACGT 3 cut(s) 605, 1736, 2278
TaqI TCGA 9 cut(s) 553, 677, 690, 714, 1348, 1661, 1739, 1791, 1854
TatI WGTACW 3 cut(s) 15, 1590, 1803
TauI GCSGC 1 cut(s) 1424
TfiI GAWTC 7 cut(s) 687, 694, 1207, 1237, 1336, 1411, 2106
Tru1I TTAA 6 cut(s) 368, 378, 407, 944, 1218, 1260
Tru9I TTAA 6 cut(s) 368, 378, 407, 944, 1218, 1260
TscAI CASTG 5 cut(s) 271, 664, 789, 1273, 1916
TseI GCWGC 2 cut(s) 462, 1765
TspDTI ATGAA 6 cut(s) 192, 727, 1078, 1536, 1660, 2276
TspGWI ACGGA 2 cut(s) 1517, 2174
TspRI CASTG 5 cut(s) 271, 664, 789, 1273, 1916
Van91I CCANNNNNTGG 5 cut(s) 680, 1247, 1316, 2057, 2147
Vha464I CTTAAG 1 cut(s) 943
VneI GTGCAC 1 cut(s) 1907
VpaK11BI GGWCC 3 cut(s) 224, 341, 1250
XbaI TCTAGA 2 cut(s) 1177, 1445
XceI RCATGY 2 cut(s) 1777, 1964
XcmI CCANNNNNNNNNTGG 1 cut(s) 894
XhoI CTCGAG 2 cut(s) 1738, 1790
XmnI GAANNNNTTC 4 cut(s) 17, 532, 1603, 1667
XspI CTAG 7 cut(s) 57, 78, 923, 1178, 1446, 1926, 2213
ZrmI AGTACT 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.