Prupe.1G074000_v2.0.a1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000058
Physical Location & Seq
Reverse (-)
5296562 .. 5300393
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G074000.1

Sequence Viewer

Length: 3366 bp
ATGTTCATGATGAAATGCCCAAAAATTCTCTTTCTTTTTGGAGTTGTATTATTGCACTACTCATGCTTCCTTCAGTCTGCCATATCTTCATCAACTTCCAACTTCACAGATCAATCTGCCCTCCTTGCCATCCAATCTAAACTCAGTTTTGACCCCACCAATATTGTTTTGGGTGGTAACTGGACTACCAAAACAAGCTTCTGTAATTGGGTTGGGGTCTTTTGTAGCAAACGCAGGCAAAGAGTCACAGCCTTAAAGCTTTCCTATATGGCTCTCCAAGGCACCATTTCCCCTCATATTGGCAACCTCTCCTTCCTCGTCTCGCTCGATCTTCGAAACAACAGCTTCTTTGGGCCCTTGCCTAATGAAATCAGCCGTTTACATCGCCTGAGGTCACTTTTATTGCATAACAACCAGCTGCAAGGAAGCATCCCTCCAACTTTGTACCATTGCCAGAATCTCGAAGTCCTAGTCCTTGAAGTAAACAGGCTAACAGGTCCTATACCTAGGGAGTTGGGTTTCCTACCTAGACTCCGTACGTTATTCCTCGGCCTGAATAATCTCACGGGTACAATTCCTTCACCCTTGGGTAACATTTCAACATTGGAAAGATTGAGCTTGGACGAGAGCGGCCTCACAGGTTCATTCTCTTCTGCTCTCCTTAACCTAACTTCTCTAACAATGATTTCTCTACCTAAGAACAGCATCTCAGGAAGTCTTCCTGTGGACCTTTGCCATTATTGGCCAAATATCCAAAACATTTCATTCTCTTTCAACAAGTTCAGTGGCAAGATCCCTTCTAGAATTGGTCAATGCAGAGAGCTTAAGATCTTATCTCTGTCATACAATAGCTTTGTTGGAAGCATCCCAGAAGAAGTTTGGAGCTTGCAACATCTTCAAATCCTATACCTTGGTGGTAATAAGCTGACAGGTACTATATCTCCTTCAATTGGAAACATGTCCAACTTGAAGAGATTTGCTGTGGGACGCAATAACATAGAGGGAAATATTCCAGGCAATTTGGGACATCTTTCTAATCTAGCTTTTTTGGGCTTGGCTTTTAATAGCCTAACAGGTCCAATACCTCAAGTAATCTTCAACATCTCCTCCTTGGAAGGTTTAGAAATGCAATCCAATGCTCTTTCTGGAGAGTTTCCTTCCTCTGCAGTTTTTCTTCCTAACCTCAATTCCTTACTTTTTCATACCAATCAAATTACAGGACCTATCCCAACTTATTTTTCAAATTTTACTAAGCTCACTCAGCTTGATGCTGACAACAACTTGCTCTATGGACCAATACCCATGAATCTTGGTAGCTTGAAACATCTCCAATTCCTTAATTTGGCAAGAAATCAGCTAACGGGAGAACCCGGGTTCGATGAGCTTAGATTCCTTTCTTCTTTGTTGAATTCTAGTTCACTGGAATATATAATTTTGTATGAAAATCCTCTAAATGGCATCATACCCGATTCCATTGGAAACTTCTCTCAGTCCCTCCAGGTAATTTATGCACCTGCATGCCAAATTAGAGGCCATATACCCAAGAGCATTGGTTCCTTGAGGAACTTGACCTTCCTTTACTTAGCTCACAACAATCTGAGTGGAAAAATACCATCATCAATTAGAGGATTGGAAACTTTGCAAAGATTGCATCTTCAAAACAATAGCATTGAAGGGTTCGTTCCGAATGAGCTTTGTCACCTGAAGAACCTAGGGGAAATATTGCTATCAAACAACAAAATCTCTGGATTGATCCCAATTTGCATAGGAAACCTCAGGCGCCTTCAAATAGTTCTCCTAGACTCCAACAGCTTGACATCATCGATACCCATGAGCTTGTGGAACCTTGAAAGTCTGTTGGTCTTAAATTTGTCATTCAATTCTTTTGATGGCAACTTACCTCAAGGAATGACAAAGTTGAATGCTGTTCAAGTGATAGATTTATCTTGGAACCAAATTGCTGGAAACATTCCAAGCGTCATTGCAGCTTTTCAAAGCCTACGCTCTCTCAATTTGTCAAACAACCTGTTTGTAGGAAACGTTCCAAAAACTTTTGGTGATCTGAAAGGGTTGGAAGATTTGGACCTATCACACAACAGTTTATCTGGCACAATACCAAAGTCTCTGGAAACACTCAAATATCTCGAGCATTTGAATTTCTCTTTCAATCATCTGTCGGGACAGATTCCTTCTGGCGGACCTTTTGCCAACTTCACTGCCCAATCATTTTTGGAGAATGGAGAACTTTGTGGGAGACCAGATTTTGGTGTCCGAAATTGCACCCCTCAAAGCACTCAAAATTATAAGGCAGCACTTCATTTGTTCAAGTATATTCTTCCAGCCATTGCCTCCACGATGATCCTTGCAGCTCTCGTTTATAGGTTGATAAAGTACCATAAAAAAAGAAATGCAGGCCTTCCAAGCTCAGTTGAACCCACGTTATCGGCGATAGAGCATAGAATGATATCATATCAAGAACTTCGACGTGCTACTAATGATTTCTGCGAAAGCAACGCACTCGGAGTAGGAAGTTTTGGTTCTGTGTACAAGGGGATACTGTCTAATGGAACAACGGTTGCTGTGAAAGTTCTAAACTTGCAAATGGAGGGTGCCTTAAAAAGTTTCGATGCGGAATGTAAGGTGTGGAGAACAATCCGGCACAGAAATCTTGTCAAGGTCATAACTTCATGCTCAAGCCATGAGGTAAGAGCTTTGGTGCTGCAATACATGTGTAATGGCAGCCTTGAGAAGTGGTTATACTCTCACAACTATTGTCTGAATCTTGTACAAAGAGTGAGCATGATGGTTGATATTGCCTTAGCTCTGGAATATCTTCACCATGGCCAAGCAGAAGTTGTGGTGCACTGTGATTTGAAGCCTAGCAATATCCTTCTCGATGAAGACATGGTTGCACATGTGGGAGACTTTGGTCTTGCAAAAATCTTGGCAAAAAATAAAGACGAAACACAAACCAGAACAATTGGTACACTTGGCTACGTTGCACCAGAATATGGTTCTACAGGAAAAGTGTCTACAAAAGGTGATGTTTATAGCTATGGGATAATGATGTTGGAAATAATCACGAGAAAGAAACCCACAGATGAAATGTTTGCCGGAGAACAGACTTTGAGACAATGGATAAATGAGTCACTCCCCAACAATTGTTTAGAAGTAGTGGATGCTGGTTTATTGAGCATAGAAGAGGGCAGAAACGTAAATGCTACAAAAAATATCATTTTGTCCATCATGGAAATAAGCTTGAGGTGTTGTGAACAAGTGCCAGAGGAAAGAATGGACTTCAAGGATGTGGTGACCAAGCTCATGAAAATAAAATTGGCACTTGGAAATAGAAGAAATAGGGTTTAG

Protein Analysis

1122

Amino Acids

123.96

Weight (kDa)

8.78

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2304
AarI CACCTGC 1 cut(s) 1522
Acc36I ACCTGC 1 cut(s) 1522
AccB1I GGYRCC 3 cut(s) 281, 1779, 2609
AccB7I CCANNNNNTGG 2 cut(s) 2264, 3011
AccBSI CCGCTC 1 cut(s) 630
AccI GTMKAC 1 cut(s) 3032
AciI CCGC 3 cut(s) 630, 2196, 2630
AclI AACGTT 1 cut(s) 2040
AclWI GGATC 3 cut(s) 787, 1747, 2353
AcoI YGGCCR 2 cut(s) 743, 2842
AcsI RAATTY 5 cut(s) 24, 1243, 1408, 1867, 2155
AcuI CTGAAG 2 cut(s) 56, 1724
AcyI GRCGYC 1 cut(s) 1780
AfaI GTAC 8 cut(s) 446, 538, 571, 934, 2393, 2546, 2787, 2986
AfiI CCNNNNNNNGG 7 cut(s) 299, 950, 1342, 1454, 2195, 2264, 3011
AflII CTTAAG 1 cut(s) 824
AflIII ACRYGT 3 cut(s) 957, 2727, 2914
AjiI CACGTC 1 cut(s) 2486
AjnI CCWGG 2 cut(s) 1012, 1497
AjuI GAANNNNNNNTTGG 6 cut(s) 182, 214, 1222, 1254, 3317, 3349
Alw21I GWGCWC 1 cut(s) 2865
Alw26I GTCTC 5 cut(s) 325, 2127, 2248, 2916, 3124
Alw44I GTGCAC 1 cut(s) 2861
AlwI GGATC 3 cut(s) 787, 1747, 2353
Ama87I CYCGRG 2 cut(s) 1370, 2144
AoxI GGCC 7 cut(s) 353, 550, 631, 743, 1531, 2413, 2842
ApaI GGGCCC 1 cut(s) 357
ApaLI GTGCAC 1 cut(s) 2861
ApeKI GCWGC 6 cut(s) 418, 1985, 2309, 2366, 2719, 2739
ApoI RAATTY 5 cut(s) 24, 1243, 1408, 1867, 2155
ArsI GACNNNNNNTTYG 2 cut(s) 456, 488
Asp700I GAANNNNTTC 1 cut(s) 2832
AspA2I CCTAGG 2 cut(s) 506, 1711
AspLEI GCGC 1 cut(s) 1782
AspS9I GGNCC 9 cut(s) 353, 354, 497, 727, 1076, 1220, 1292, 2083, 2198
AsuC2I CCSGG 2 cut(s) 1371, 1372
AsuHPI GGTGA 6 cut(s) 573, 1691, 2069, 2828, 3053, 3322
AsuII TTCGAA 1 cut(s) 334
AvaI CYCGRG 2 cut(s) 1370, 2144
AvaII GGWCC 7 cut(s) 497, 727, 1076, 1220, 1292, 2083, 2198
AvrII CCTAGG 2 cut(s) 506, 1711
AxyI CCTNAGG 2 cut(s) 389, 1775
BaeGI GKGCMC 2 cut(s) 357, 2865
BalI TGGCCA 2 cut(s) 745, 2844
BanI GGYRCC 3 cut(s) 281, 1779, 2609
BanII GRGCYC 1 cut(s) 357
BarI GAAGNNNNNNTAC 4 cut(s) 562, 594, 2741, 2773
BauI CACGAG 1 cut(s) 3082
BbsI GAAGAC 2 cut(s) 710, 2907
Bbv12I GWGCWC 1 cut(s) 2865
BbvI GCAGC 6 cut(s) 405, 1997, 2321, 2378, 2706, 2751
BccI CCATC 5 cut(s) 137, 1621, 1883, 2797, 3251
BceAI ACGGC 1 cut(s) 360
BcgI CGANNNNNNTGC 2 cut(s) 2500, 2534
BciT130I CCWGG 2 cut(s) 1014, 1499
BciVI GTATCC 1 cut(s) 2547
BcnI CCSGG 2 cut(s) 1371, 1372
BcoDI GTCTC 5 cut(s) 325, 2127, 2248, 2916, 3124
BfaI CTAG 9 cut(s) 470, 507, 528, 801, 1040, 1413, 1712, 1799, 2880
BfmI CTRYAG 2 cut(s) 1164, 3018
BfoI RGCGCY 1 cut(s) 1783
BfrI CTTAAG 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 1522
BfuI GTATCC 1 cut(s) 2547
BglII AGATCT 1 cut(s) 828
BisI GCNGC 7 cut(s) 419, 631, 1986, 2310, 2367, 2720, 2740
BlnI CCTAGG 2 cut(s) 506, 1711
BlsI GCNGC 7 cut(s) 420, 632, 1987, 2311, 2368, 2721, 2741
Bme1390I CCNGG 4 cut(s) 1014, 1371, 1372, 1499
Bme18I GGWCC 7 cut(s) 497, 727, 1076, 1220, 1292, 2083, 2198
BmeT110I CYCGRG 2 cut(s) 1370, 2144
BmgBI CACGTC 1 cut(s) 2486
BmgT120I GGNCC 9 cut(s) 353, 354, 497, 727, 1076, 1220, 1292, 2083, 2198
BmiI GGNNCC 7 cut(s) 283, 355, 1555, 1781, 1844, 1952, 2611
BmrFI CCNGG 4 cut(s) 1014, 1371, 1372, 1499
BmsI GCATC 8 cut(s) 438, 714, 873, 1258, 1467, 1660, 2617, 3169
BoxI GACNNNNGTC 1 cut(s) 2928
BpiI GAAGAC 2 cut(s) 710, 2907
BpmI CTGGAG 2 cut(s) 1167, 1481
Bpu10I CCTNAGC 1 cut(s) 2818
Bpu14I TTCGAA 1 cut(s) 334
BpuEI CTTGAG 6 cut(s) 1071, 1579, 1887, 2677, 2765, 3280
BpuMI CCSGG 2 cut(s) 1371, 1372
Bsa29I ATCGAT 1 cut(s) 1823
BsaHI GRCGYC 1 cut(s) 1780
BsaI GGTCTC 1 cut(s) 2248
BsaJI CCNNGG 9 cut(s) 277, 506, 547, 585, 910, 1110, 1370, 1711, 2839
BsaXI ACNNNNNCTCC 6 cut(s) 516, 546, 925, 955, 1091, 1121
Bsc4I CCNNNNNNNGG 7 cut(s) 299, 950, 1342, 1454, 2195, 2264, 3011
Bse1I ACTGG 2 cut(s) 185, 1425
Bse21I CCTNAGG 2 cut(s) 389, 1775
Bse3DI GCAATG 3 cut(s) 448, 1980, 2343
BseBI CCWGG 2 cut(s) 1014, 1499
BseCI ATCGAT 1 cut(s) 1823
BseDI CCNNGG 9 cut(s) 277, 506, 547, 585, 910, 1110, 1370, 1711, 2839
BseGI GGATG 5 cut(s) 129, 429, 864, 3184, 3310
BseLI CCNNNNNNNGG 7 cut(s) 299, 950, 1342, 1454, 2195, 2264, 3011
BseMI GCAATG 3 cut(s) 448, 1980, 2343
BseMII CTCAG 8 cut(s) 157, 380, 723, 1274, 1502, 1589, 1789, 2439
BseNI ACTGG 2 cut(s) 185, 1425
BseRI GAGGAG 1 cut(s) 1096
BseSI GKGCMC 2 cut(s) 357, 2865
BseXI GCAGC 6 cut(s) 405, 1997, 2321, 2378, 2706, 2751
BshFI GGCC 7 cut(s) 355, 552, 633, 745, 1533, 2415, 2844
BshNI GGYRCC 3 cut(s) 281, 1779, 2609
BshVI ATCGAT 1 cut(s) 1823
BsiHKAI GWGCWC 1 cut(s) 2865
BsiHKCI CYCGRG 2 cut(s) 1370, 2144
BsiSI CCGG 3 cut(s) 1371, 2656, 3114
BsiWI CGTACG 1 cut(s) 536
BslFI GGGAC 4 cut(s) 999, 1038, 1477, 2193
BslI CCNNNNNNNGG 7 cut(s) 299, 950, 1342, 1454, 2195, 2264, 3011
BsmAI GTCTC 5 cut(s) 325, 2127, 2248, 2916, 3124
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 4 cut(s) 999, 1038, 1477, 2193
BsmI GAATGC 1 cut(s) 1927
BsnI GGCC 7 cut(s) 355, 552, 633, 745, 1533, 2415, 2844
Bso31I GGTCTC 1 cut(s) 2248
BsoBI CYCGRG 2 cut(s) 1370, 2144
Bsp119I TTCGAA 1 cut(s) 334
Bsp120I GGGCCC 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 357, 2865
Bsp1407I TGTACA 2 cut(s) 2544, 2785
Bsp143I GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
Bsp19I CCATGG 1 cut(s) 2839
BspACI CCGC 3 cut(s) 630, 2196, 2630
BspANI GGCC 7 cut(s) 355, 552, 633, 745, 1533, 2415, 2844
BspCNI CTCAG 8 cut(s) 156, 381, 722, 1273, 1501, 1590, 1788, 2438
BspDI ATCGAT 1 cut(s) 1823
BspHI TCATGA 2 cut(s) 6, 3321
BspLI GGNNCC 7 cut(s) 283, 355, 1555, 1781, 1844, 1952, 2611
BspMAI CTGCAG 1 cut(s) 1168
BspMI ACCTGC 1 cut(s) 1522
BspPI GGATC 3 cut(s) 787, 1747, 2353
BspT104I TTCGAA 1 cut(s) 334
BspT107I GGYRCC 3 cut(s) 281, 1779, 2609
BspTI CTTAAG 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 2248
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 3 cut(s) 448, 1980, 2343
BsrGI TGTACA 2 cut(s) 2544, 2785
BsrI ACTGG 2 cut(s) 185, 1425
BssECI CCNNGG 9 cut(s) 277, 506, 547, 585, 910, 1110, 1370, 1711, 2839
BssMI GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
BssNI GRCGYC 1 cut(s) 1780
BssSI CACGAG 1 cut(s) 3082
BssT1I CCWWGG 7 cut(s) 277, 506, 585, 910, 1110, 1711, 2839
Bst2BI CACGAG 1 cut(s) 3082
Bst2UI CCWGG 2 cut(s) 1014, 1499
Bst4CI ACNGT 4 cut(s) 2099, 2559, 2575, 2867
Bst6I CTCTTC 3 cut(s) 655, 965, 3195
BstACI GRCGYC 1 cut(s) 1780
BstAFI CTTAAG 1 cut(s) 824
BstAPI GCANNNNNTGC 1 cut(s) 1648
BstAUI TGTACA 2 cut(s) 2544, 2785
BstBI TTCGAA 1 cut(s) 334
BstC8I GCNNGC 4 cut(s) 236, 887, 1519, 2413
BstDSI CCRYGG 1 cut(s) 2839
BstEII GGTNACC 1 cut(s) 3310
BstF5I GGATG 5 cut(s) 129, 429, 864, 3184, 3310
BstH2I RGCGCY 1 cut(s) 1783
BstHHI GCGC 1 cut(s) 1782
BstKTI GATC 7 cut(s) 112, 331, 795, 831, 1755, 2062, 2361
BstMAI GTCTC 5 cut(s) 325, 2127, 2248, 2916, 3124
BstMBI GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
BstMWI GCNNNNNNNGC 4 cut(s) 125, 1261, 1648, 2421
BstNI CCWGG 2 cut(s) 1014, 1499
BstNSI RCATGY 4 cut(s) 961, 1521, 2731, 2918
BstPAI GACNNNNGTC 1 cut(s) 2928
BstPI GGTNACC 1 cut(s) 3310
BstSCI CCNGG 4 cut(s) 1012, 1369, 1370, 1497
BstSFI CTRYAG 2 cut(s) 1164, 3018
BstSLI GKGCMC 2 cut(s) 357, 2865
BstV1I GCAGC 6 cut(s) 405, 1997, 2321, 2378, 2706, 2751
BstV2I GAAGAC 2 cut(s) 710, 2907
BstX2I RGATCY 2 cut(s) 792, 828
BstXI CCANNNNNNTGG 1 cut(s) 1961
BstYI RGATCY 2 cut(s) 792, 828
Bsu15I ATCGAT 1 cut(s) 1823
Bsu36I CCTNAGG 2 cut(s) 389, 1775
BsuI GTATCC 1 cut(s) 2547
BsuRI GGCC 7 cut(s) 355, 552, 633, 745, 1533, 2415, 2844
BsuTUI ATCGAT 1 cut(s) 1823
BtgI CCRYGG 1 cut(s) 2839
BtgZI GCGATG 1 cut(s) 368
BtrI CACGTC 1 cut(s) 2486
BtsCI GGATG 5 cut(s) 129, 429, 864, 3184, 3310
BtsI GCAGTG 1 cut(s) 2214
BtsIMutI CAGTG 4 cut(s) 790, 1418, 2214, 2863
BveI ACCTGC 1 cut(s) 1522
Cac8I GCNNGC 4 cut(s) 236, 887, 1519, 2413
CciI TCATGA 2 cut(s) 6, 3321
CfoI GCGC 1 cut(s) 1782
Cfr13I GGNCC 9 cut(s) 353, 354, 497, 727, 1076, 1220, 1292, 2083, 2198
Cfr9I CCCGGG 1 cut(s) 1370
ClaI ATCGAT 1 cut(s) 1823
CseI GACGC 2 cut(s) 996, 1966
Csp6I GTAC 8 cut(s) 445, 537, 570, 933, 2392, 2545, 2786, 2985
CspCI CAANNNNNGTGG 6 cut(s) 145, 180, 766, 801, 1582, 1617
CviQI GTAC 8 cut(s) 445, 537, 570, 933, 2392, 2545, 2786, 2985
DinI GGCGCC 1 cut(s) 1781
DpnI GATC 7 cut(s) 111, 330, 794, 830, 1754, 2061, 2360
DpnII GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
EaeI YGGCCR 2 cut(s) 743, 2842
Eam1104I CTCTTC 3 cut(s) 655, 965, 3195
EarI CTCTTC 3 cut(s) 655, 965, 3195
EciI GGCGGA 1 cut(s) 2211
Eco130I CCWWGG 7 cut(s) 277, 506, 585, 910, 1110, 1711, 2839
Eco147I AGGCCT 1 cut(s) 2415
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 2248
Eco32I GATATC 1 cut(s) 2466
Eco47I GGWCC 7 cut(s) 497, 727, 1076, 1220, 1292, 2083, 2198
Eco57I CTGAAG 2 cut(s) 56, 1724
Eco81I CCTNAGG 2 cut(s) 389, 1775
Eco88I CYCGRG 2 cut(s) 1370, 2144
Eco91I GGTNACC 1 cut(s) 3310
EcoO109I RGGNCCY 3 cut(s) 354, 497, 1220
EcoO65I GGTNACC 1 cut(s) 3310
EcoRI GAATTC 1 cut(s) 1408
EcoRII CCWGG 2 cut(s) 1012, 1497
EcoRV GATATC 1 cut(s) 2466
EcoT14I CCWWGG 7 cut(s) 277, 506, 585, 910, 1110, 1711, 2839
EcoT38I GRGCYC 1 cut(s) 357
EgeI GGCGCC 1 cut(s) 1781
EheI GGCGCC 1 cut(s) 1781
ErhI CCWWGG 7 cut(s) 277, 506, 585, 910, 1110, 1711, 2839
Esp3I CGTCTC 1 cut(s) 325
FaqI GGGAC 4 cut(s) 999, 1038, 1477, 2193
FblI GTMKAC 1 cut(s) 3032
Fnu4HI GCNGC 7 cut(s) 419, 631, 1986, 2310, 2367, 2720, 2740
FokI GGATG 5 cut(s) 116, 416, 851, 3191, 3317
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 7 cut(s) 419, 631, 1986, 2310, 2367, 2720, 2740
FspBI CTAG 9 cut(s) 470, 507, 528, 801, 1040, 1413, 1712, 1799, 2880
GlaI GCGC 1 cut(s) 1781
GluI GCNGC 7 cut(s) 419, 631, 1986, 2310, 2367, 2720, 2740
GsuI CTGGAG 2 cut(s) 1167, 1481
HaeII RGCGCY 1 cut(s) 1783
HaeIII GGCC 7 cut(s) 355, 552, 633, 745, 1533, 2415, 2844
HapII CCGG 3 cut(s) 1371, 2656, 3114
HgaI GACGC 2 cut(s) 996, 1966
HhaI GCGC 1 cut(s) 1782
Hin1I GRCGYC 1 cut(s) 1780
Hin6I GCGC 1 cut(s) 1780
HinP1I GCGC 1 cut(s) 1780
HindIII AAGCTT 3 cut(s) 196, 257, 3256
HpaII CCGG 3 cut(s) 1371, 2656, 3114
HphI GGTGA 6 cut(s) 573, 1691, 2069, 2828, 3053, 3322
Hpy166II GTNNAC 9 cut(s) 380, 484, 727, 1418, 2545, 2863, 2987, 3033, 3272
Hpy188I TCNGA 6 cut(s) 1599, 1686, 2064, 2273, 2522, 2778
Hpy8I GTNNAC 9 cut(s) 380, 484, 727, 1418, 2545, 2863, 2987, 3033, 3272
Hpy99I CGWCG 1 cut(s) 2487
HpyCH4III ACNGT 4 cut(s) 2099, 2559, 2575, 2867
HpyCH4IV ACGT 6 cut(s) 539, 2040, 2438, 2485, 2997, 3213
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 1261, 1648, 2421
HpySE526I ACGT 6 cut(s) 539, 2040, 2438, 2485, 2997, 3213
Hsp92I GRCGYC 1 cut(s) 1780
HspAI GCGC 1 cut(s) 1780
KasI GGCGCC 1 cut(s) 1779
Kzo9I GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
LmnI GCTCC 1 cut(s) 882
Lsp1109I GCAGC 6 cut(s) 405, 1997, 2321, 2378, 2706, 2751
LweI GCATC 8 cut(s) 438, 714, 873, 1258, 1467, 1660, 2617, 3169
MaeI CTAG 9 cut(s) 470, 507, 528, 801, 1040, 1413, 1712, 1799, 2880
MaeII ACGT 6 cut(s) 539, 2040, 2438, 2485, 2997, 3213
MaeIII GTNAC 7 cut(s) 176, 244, 393, 590, 1697, 3147, 3310
MalI GATC 7 cut(s) 111, 330, 794, 830, 1754, 2061, 2360
MbiI CCGCTC 1 cut(s) 630
MboI GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
MfeI CAATTG 3 cut(s) 948, 2979, 3160
MflI RGATCY 2 cut(s) 792, 828
MhlI GDGCHC 2 cut(s) 357, 2865
MlsI TGGCCA 2 cut(s) 745, 2844
MluNI TGGCCA 2 cut(s) 745, 2844
Mly113I GGCGCC 1 cut(s) 1780
MlyI GAGTC 4 cut(s) 252, 525, 1796, 3155
MmeI TCCRAC 7 cut(s) 123, 461, 838, 987, 1830, 2052, 3051
Mox20I TGGCCA 2 cut(s) 745, 2844
MroXI GAANNNNTTC 1 cut(s) 2832
MscI TGGCCA 2 cut(s) 745, 2844
MseI TTAA 7 cut(s) 254, 663, 825, 1062, 1338, 1865, 2615
MslI CAYNNNNRTG 1 cut(s) 1516
Msp20I TGGCCA 2 cut(s) 745, 2844
MspA1I CMGCKG 1 cut(s) 418
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 3 cut(s) 1371, 2656, 3114
MspR9I CCNGG 4 cut(s) 1014, 1371, 1372, 1499
MunI CAATTG 3 cut(s) 948, 2979, 3160
Mva1269I GAATGC 1 cut(s) 1927
MvaI CCWGG 2 cut(s) 1014, 1499
MwoI GCNNNNNNNGC 4 cut(s) 125, 1261, 1648, 2421
NarI GGCGCC 1 cut(s) 1780
NciI CCSGG 2 cut(s) 1371, 1372
NcoI CCATGG 1 cut(s) 2839
NdeII GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
NlaIV GGNNCC 7 cut(s) 283, 355, 1555, 1781, 1844, 1952, 2611
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 5 cut(s) 244, 393, 1697, 3147, 3310
NspI RCATGY 4 cut(s) 961, 1521, 2731, 2918
NspV TTCGAA 1 cut(s) 334
PaeI GCATGC 1 cut(s) 1521
PaeR7I CTCGAG 1 cut(s) 2144
PagI TCATGA 2 cut(s) 6, 3321
PaqCI CACCTGC 1 cut(s) 1522
PceI AGGCCT 1 cut(s) 2415
PciI ACATGT 3 cut(s) 957, 2727, 2914
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 2444
PctI GAATGC 1 cut(s) 1927
PdmI GAANNNNTTC 1 cut(s) 2832
PfeI GAWTC 6 cut(s) 457, 1306, 1389, 1469, 2185, 2779
Pfl23II CGTACG 1 cut(s) 536
PflMI CCANNNNNTGG 2 cut(s) 2264, 3011
PkrI GCNGC 7 cut(s) 420, 632, 1987, 2311, 2368, 2721, 2741
PleI GAGTC 4 cut(s) 251, 525, 1796, 3154
PluTI GGCGCC 1 cut(s) 1783
PpsI GAGTC 4 cut(s) 251, 525, 1796, 3154
PpuMI RGGWCCY 2 cut(s) 497, 1220
PscI ACATGT 3 cut(s) 957, 2727, 2914
PshAI GACNNNNGTC 1 cut(s) 2928
PsiI TTATAA 1 cut(s) 2304
Psp1406I AACGTT 1 cut(s) 2040
Psp5II RGGWCCY 2 cut(s) 497, 1220
Psp6I CCWGG 2 cut(s) 1012, 1497
PspEI GGTNACC 1 cut(s) 3310
PspGI CCWGG 2 cut(s) 1012, 1497
PspLI CGTACG 1 cut(s) 536
PspN4I GGNNCC 7 cut(s) 283, 355, 1555, 1781, 1844, 1952, 2611
PspOMI GGGCCC 1 cut(s) 353
PspPI GGNCC 9 cut(s) 353, 354, 497, 727, 1076, 1220, 1292, 2083, 2198
PspPPI RGGWCCY 2 cut(s) 497, 1220
PsrI GAACNNNNNNTAC 4 cut(s) 2025, 2057, 2968, 3000
PstI CTGCAG 1 cut(s) 1168
PsuI RGATCY 2 cut(s) 792, 828
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 8 cut(s) 446, 538, 571, 934, 2393, 2546, 2787, 2986
RsaNI GTAC 8 cut(s) 445, 537, 570, 933, 2392, 2545, 2786, 2985
RseI CAYNNNNRTG 1 cut(s) 1516
SaqAI TTAA 7 cut(s) 254, 663, 825, 1062, 1338, 1865, 2615
SatI GCNGC 7 cut(s) 419, 631, 1986, 2310, 2367, 2720, 2740
Sau3AI GATC 7 cut(s) 109, 328, 792, 828, 1752, 2059, 2358
Sau96I GGNCC 9 cut(s) 353, 354, 497, 727, 1076, 1220, 1292, 2083, 2198
SchI GAGTC 4 cut(s) 252, 525, 1796, 3155
ScrFI CCNGG 4 cut(s) 1014, 1371, 1372, 1499
SduI GDGCHC 2 cut(s) 357, 2865
SfaNI GCATC 8 cut(s) 438, 714, 873, 1258, 1467, 1660, 2617, 3169
SfcI CTRYAG 2 cut(s) 1164, 3018
SfoI GGCGCC 1 cut(s) 1781
Sfr274I CTCGAG 1 cut(s) 2144
SfuI TTCGAA 1 cut(s) 334
SinI GGWCC 7 cut(s) 497, 727, 1076, 1220, 1292, 2083, 2198
SlaI CTCGAG 1 cut(s) 2144
SmaI CCCGGG 1 cut(s) 1372
SmiMI CAYNNNNRTG 1 cut(s) 1516
SmlI CTYRAG 8 cut(s) 824, 1086, 1558, 1902, 2144, 2692, 2744, 3259
SmoI CTYRAG 8 cut(s) 824, 1086, 1558, 1902, 2144, 2692, 2744, 3259
SphI GCATGC 1 cut(s) 1521
SseBI AGGCCT 1 cut(s) 2415
SsiI CCGC 3 cut(s) 630, 2196, 2630
SspDI GGCGCC 1 cut(s) 1779
SspI AATATT 3 cut(s) 163, 1009, 1722
SspMI CTAG 9 cut(s) 470, 507, 528, 801, 1040, 1413, 1712, 1799, 2880
StuI AGGCCT 1 cut(s) 2415
StyD4I CCNGG 4 cut(s) 1012, 1369, 1370, 1497
StyI CCWWGG 7 cut(s) 277, 506, 585, 910, 1110, 1711, 2839
TaaI ACNGT 4 cut(s) 2099, 2559, 2575, 2867
TaiI ACGT 6 cut(s) 542, 2043, 2441, 2488, 3000, 3216
TaqI TCGA 9 cut(s) 327, 334, 462, 1377, 1823, 2145, 2482, 2625, 2895
TatI WGTACW 2 cut(s) 2544, 2785
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 6 cut(s) 457, 1306, 1389, 1469, 2185, 2779
Tru1I TTAA 7 cut(s) 254, 663, 825, 1062, 1338, 1865, 2615
Tru9I TTAA 7 cut(s) 254, 663, 825, 1062, 1338, 1865, 2615
TscAI CASTG 4 cut(s) 790, 1425, 2221, 2870
TseFI GTSAC 5 cut(s) 244, 393, 1697, 3147, 3310
TseI GCWGC 6 cut(s) 418, 1985, 2309, 2366, 2719, 2739
Tsp45I GTSAC 5 cut(s) 244, 393, 1697, 3147, 3310
TspGWI ACGGA 1 cut(s) 524
TspMI CCCGGG 1 cut(s) 1370
TspRI CASTG 4 cut(s) 790, 1425, 2221, 2870
Van91I CCANNNNNTGG 2 cut(s) 2264, 3011
Vha464I CTTAAG 1 cut(s) 824
VneI GTGCAC 1 cut(s) 2861
VpaK11BI GGWCC 7 cut(s) 497, 727, 1076, 1220, 1292, 2083, 2198
XapI RAATTY 5 cut(s) 24, 1243, 1408, 1867, 2155
XbaI TCTAGA 1 cut(s) 800
XceI RCATGY 4 cut(s) 961, 1521, 2731, 2918
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 876
XhoI CTCGAG 1 cut(s) 2144
XmaI CCCGGG 1 cut(s) 1370
XmaJI CCTAGG 2 cut(s) 506, 1711
XmiI GTMKAC 1 cut(s) 3032
XmnI GAANNNNTTC 1 cut(s) 2832
XspI CTAG 9 cut(s) 470, 507, 528, 801, 1040, 1413, 1712, 1799, 2880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.