Rroxscaffold_1G00060620

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000058
Physical Location & Seq
Forward (+)
82882131 .. 82885890
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060620.1

Sequence Viewer

Length: 3240 bp
ATGGCTGCAGCGCACACCAACTTCAGCACCGACCAGTCTGCTCTTCTTGCTCTCAAAGCACATATCACCGTCGACCCTCAGAACAAGATCTTGACCAACTGGTCATCCAACTCCGACGTTTGCAACTGGGTCGGGGTTACATGCGGTGAACGCCACCTTAGAGTCGTATTTTTGAACCTTTCTGTGTTTCATCTCACTGGCACCATTCCTCCAGAGCTAGGAAACCTGTCATTTCTTGCTGGGATGCGCCTAGAAAATAATAGCTTCCATGGAACTATCCCAGGGGAATTGGCTGCTTTGCGGCGACTCACGTTGTTTAGCTTCGGATTCAATAACTTTGTGGGAGAGATTCCATCATGGTTGGGGTCCTTGTCCAAACTACAAATCCTCAATTTGTATGGCAATGGGTTTTCAGGTTCAATACCCACCGTTATCTTCAACTTGTCTGCACTACAAGTTCTTGATCTCAGATATAATGAACTATCAGAACTTCCAAAGGAGATTGGTGCTTTAGATTTGGAGGAATTGTTTGTGCAAGACAACGGCCTAGAAGGGCTTGTTCCTGCCGGTGTCTTCAACATGTCTTCTTTGACTACCCTGAGTCTATCTCGAAACCGTTTGAACGGCAAGGTTCCAGACAATTTGTGTGGGAATCTCCCTAATCTTCAAGGATTGAATTTGGCTTACAACCAATTTGAGGGTCCACTTCCATCAAGCTTAGAGCAGTGCAAACAACTTCTGGTATTAAGATTGGGGTCTAACAATTTCAGTGGAAGTATACCTAGAAATATTGGGAACTTGACGCAGATCAAGCATCTTCATCTTGGTTCAAACAATTTGACAGGGACAATACCACACGAGATTGGTCACCTTGGAAACTTAGAGATTTTAGCACTTAGTGTGAATAATCTCAAAGGCCTCGTCCCATCAGAAATCTTCAATTTATCCTTGCTAACAGGAATCGATATTTCTTTGAATCAACTCTCAGGCAGTCTCCCTGCAAATATAGGCCTTGTCATTCCAAACCTACAAGAGATCCATGTAGGAGGAAATAATCTTAGCGGAGAGATCCCAAAATTCATCTCCAATGCTTCTAAGCTCACAAAACTAGACATGGGCCCAAACTTATTTTCTGGGTTTATTCCTGCTACCCTCTGTGCCTTGCCAAATCTTCAATGGCTTCTATTATCGTTGAATAACTTGATGATTGATATTTCTACCCCAGAAGTAAACGTTTTCTCCTGTCTACCTAATCTCAGAAATTTGAGGATGTTAAGCCTCCCAGGAAACCCTTTAAATACCACCCTCCCTAGTTCCCTTGGGAATCTCTCTACATCACTACAATATATGGATTTTAGAGGATGCAACTTAAGGGGTAACATTCCCAACGATATTGGCAACTTAAGCGGCTTGACAACCTTGTACCTGGCATTCAATGAACTGAGTGGATCAATTCCAACGACACTGGGAAAACTACGGAATCTCCAAGGTTTGTATTTGACTTTTAACAAATTGCAAGGAAACATACCAGATGAACTTTGTCAGCTAGACAGCCTAGCTGATTTAGAATTGTATATTAATTTACTCTCTGGTACTATACCTTCCTGCTTAGGTAACCTAACCAGATCACTAAGAAGGCTAAATTTAGGATACAATATGCTAACTTCCACAATACCTTCTTCCTTGTGGGAACTTAGATACATCTTGCTCTTAGGTCTGTCATCCAATAGTCTAAGTGGACCTATCTCAGAAGATGTTGGCAATTTGGAAGTTGTGGTAGATATAGATTTATCAAACAACCATTTATCAGGTTTGATACCAAGCAGCTTTGGGGGTCTTGAAAATTTGGTCTCACTCTCCTTGGCAAATAATAATTTTGAAGGCAACATTCCTAGTTCATTTGGGAACTCCCTAAGCCTAGAAATCCTTGATTTATCCAACAACAGTCTTTCTGGAGTCATTCCTAAGTCTCTAGAAGCACTCTCACATCTCCAATCTCTGAATTTGTCTTTCAACAAACTACAAGGAGAAATTCCAACTGGTGGACCTTTTGAAAACTTCTCTTCTGCATCTTTTGACTCAAATGGTGCACTCTGTGGAGCACCCCAATTGCTTGTTCCACCATGCAAGAAGAAAGCAGGTGGCAGTTCTTTCCCAAAATATATTATTATAGGTATCTTGTCAGCAATAGTGCTCATAGTAGGCCTTGCATCGATGATGATACTATGCAGAAAAAGGAATGTGGAAGCTGCAACAGAGATTGCCCTGTTACATCAGCCTCTTTGGAGAAATGTTTCACACTTGGAACTTTTAAGGGCAACAAATGGGTTCAATGAAAGCAACTTACTTGGCTCAGGGGGTTTTGGCACAGTATATAAAGGAACGTTGTCAGATGGGATAGATGTTGCGGTAAAGGTTTTCAATTTAGAAGTAGAAGGGGCTTTCAAGAGTTTTCATAATGAATGTGAAATGCTAGGCACTATTCGTCATCGAAATCTTCTCAAAATAATCAGTTGTTGCAGTCAAACAAACTTCAAAGCCGCAGTACTGAAGTACATGCCCAATGGGAGTCTTGATAAATGGTTGTATTCCGAAAACCTTTCCTTGAACCTCCTGCAGAGGTTGAATATAGTGATTGATGTTGCATCAGCGTTGGAGTACCTTCATCATGGTTATGAAACACCCATCACCCATTGTGATTTGAAGCCCAGAAACATACTACTAGATGATGATATGGTCGCACATGTTGCTGATTTTGGCATGGCAAAGCTTTTAGGTGGTGGAGATTCTATGACTCAGACAATGACCCTAGCCACCATTGGTTATATGGCTCCAGAGTATGGAACAGAAGGAATAGTCTCGAGAAGAGGGGATGTGTACAGCTTTGGTATTGTACTGATGGAAACATTCACAAAGAGGAAGCCAACAGATGAGATGTTTGGTGGGGATATGAGTATGAAGCAATGGGTTGCAAATTCACTCTTTCTAGATGGAATGGTTGAAGTTGCAGATGCCCATTTATTTGGGACTCAAGAAGGCCATGATGTTGTGAGCACGGCGGAGTGTTTATCATCCATTCTGGGATTAGCTCTAGCTTGCTGTGCAGAATCACCGGAAGAGAGAACAAATATGCAAGATGCTCTAGCCACGCTCAACAAAATTAAGACCAAGTTTTTGATGGCCACTGCAGGAGGTGTGGTGTTAAACAGTCCTCTTATAAGCAGTTCTGGATTAAATTGA

Protein Analysis

1079

Amino Acids

116.43

Weight (kDa)

5.45

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 10 - 48 9.2e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 121 - 225 1.1e-09 Leucine-rich repeat region
LRR_8 PF13855 173 - 232 3.2e-07 Leucine rich repeat
LRR_14 PF23598 217 - 416 3.1e-11 Leucine-rich repeat region
LRR_8 PF13855 341 - 401 5.3e-07 Leucine rich repeat
LRR_14 PF23598 414 - 547 3.1e-11 Leucine-rich repeat region
LRR_14 PF23598 561 - 674 7.1e-08 Leucine-rich repeat region
LRR_8 PF13855 615 - 674 5.9e-09 Leucine rich repeat
Pkinase PF00069 778 - 1049 9.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 781 - 1048 1.4e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3218
AarI CACCTGC 1 cut(s) 2129
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 2129
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 2 cut(s) 2042, 2840
AccI GTMKAC 3 cut(s) 72, 778, 1246
AciI CCGC 7 cut(s) 144, 301, 1062, 1407, 2408, 2541, 3059
AclI AACGTT 2 cut(s) 1233, 2384
AclWI GGATC 3 cut(s) 1030, 1063, 1456
AcoI YGGCCR 1 cut(s) 3180
AcsI RAATTY 8 cut(s) 676, 1076, 1261, 1642, 1843, 2002, 2031, 2974
AcuI CTGAAG 2 cut(s) 7, 2570
AdeI CACNNNGTG 2 cut(s) 899, 2096
AfaI GTAC 7 cut(s) 1424, 1594, 2547, 2555, 2660, 2879, 2895
AfiI CCNNNNNNNGG 4 cut(s) 218, 697, 2042, 2840
AflII CTTAAG 2 cut(s) 1369, 1402
AflIII ACRYGT 2 cut(s) 579, 2743
AjnI CCWGG 3 cut(s) 280, 1282, 1425
AjuI GAANNNNNNNTTGG 4 cut(s) 1114, 1146, 2100, 2132
Alw21I GWGCWC 4 cut(s) 2092, 2104, 2196, 3056
Alw26I GTCTC 4 cut(s) 998, 1855, 1974, 2863
Alw44I GTGCAC 1 cut(s) 2088
AlwI GGATC 3 cut(s) 1030, 1063, 1456
Ama87I CYCGRG 1 cut(s) 2860
AoxI GGCC 7 cut(s) 544, 916, 1009, 1117, 2203, 3037, 3180
ApaI GGGCCC 1 cut(s) 1121
ApaLI GTGCAC 1 cut(s) 2088
ApeKI GCWGC 5 cut(s) 5, 8, 293, 1824, 2249
ApoI RAATTY 8 cut(s) 676, 1076, 1261, 1642, 1843, 2002, 2031, 2974
ArsI GACNNNNNNTTYG 4 cut(s) 906, 938, 3157, 3189
AseI ATTAAT 1 cut(s) 1578
Asp700I GAANNNNTTC 1 cut(s) 2293
AspLEI GCGC 2 cut(s) 13, 249
AspS9I GGNCC 6 cut(s) 366, 701, 1117, 1118, 1739, 2045
AsuHPI GGTGA 5 cut(s) 58, 158, 860, 2680, 3102
AvaI CYCGRG 1 cut(s) 2860
AvaII GGWCC 4 cut(s) 366, 701, 1739, 2045
BaeGI GKGCMC 2 cut(s) 1121, 2092
BalI TGGCCA 1 cut(s) 3182
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 1121
BarI GAAGNNNNNNTAC 2 cut(s) 1585, 1617
BauI CACGAG 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 565, 576
Bbv12I GWGCWC 4 cut(s) 2092, 2104, 2196, 3056
BbvI GCAGC 4 cut(s) 20, 280, 1836, 2236
BccI CCATC 8 cut(s) 361, 718, 934, 2387, 2693, 2893, 2984, 3172
BceAI ACGGC 3 cut(s) 559, 640, 3072
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 112, 146
BciT130I CCWGG 3 cut(s) 282, 1284, 1427
BciVI GTATCC 1 cut(s) 1643
BcoDI GTCTC 4 cut(s) 998, 1855, 1974, 2863
BfmI CTRYAG 3 cut(s) 6, 2615, 3186
BfrI CTTAAG 2 cut(s) 1369, 1402
BfuAI ACCTGC 1 cut(s) 2129
BfuI GTATCC 1 cut(s) 1643
BglII AGATCT 1 cut(s) 87
BisI GCNGC 8 cut(s) 6, 9, 294, 302, 1408, 1825, 2250, 2541
BlsI GCNGC 8 cut(s) 7, 10, 295, 303, 1409, 1826, 2251, 2542
BmcAI AGTACT 1 cut(s) 2547
Bme1390I CCNGG 3 cut(s) 282, 1284, 1427
Bme18I GGWCC 4 cut(s) 366, 701, 1739, 2045
BmeT110I CYCGRG 1 cut(s) 2860
BmgT120I GGNCC 6 cut(s) 366, 701, 1117, 1118, 1739, 2045
BmiI GGNNCC 6 cut(s) 202, 367, 633, 702, 1119, 2832
BmrFI CCNGG 3 cut(s) 282, 1284, 1427
BmrI ACTGGG 2 cut(s) 136, 1475
BmsI GCATC 8 cut(s) 234, 823, 1352, 2078, 2219, 2654, 3001, 3127
BmuI ACTGGG 2 cut(s) 136, 1475
BpiI GAAGAC 2 cut(s) 565, 576
BplI GAGNNNNNCTC 2 cut(s) 592, 624
BpmI CTGGAG 3 cut(s) 195, 1974, 2817
Bpu10I CCTNAGC 3 cut(s) 1609, 1913, 2353
BpuEI CTTGAG 1 cut(s) 3015
Bsa29I ATCGAT 2 cut(s) 963, 2213
BsaI GGTCTC 1 cut(s) 1855
BsaJI CCNNGG 8 cut(s) 268, 280, 281, 871, 1282, 1318, 1486, 1860
BsaWI WCCGGW 1 cut(s) 3112
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 697, 2042, 2840
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 6 cut(s) 34, 104, 131, 202, 1470, 2044
Bse3DI GCAATG 2 cut(s) 409, 2969
BseBI CCWGG 3 cut(s) 282, 1284, 1427
BseCI ATCGAT 2 cut(s) 963, 2213
BseDI CCNNGG 8 cut(s) 268, 280, 281, 871, 1282, 1318, 1486, 1860
BseGI GGATG 7 cut(s) 104, 249, 1275, 1367, 1721, 2878, 3071
BseLI CCNNNNNNNGG 4 cut(s) 218, 697, 2042, 2840
BseMI GCAATG 2 cut(s) 409, 2969
BseMII CTCAG 9 cut(s) 92, 481, 590, 999, 1270, 1433, 1761, 2367, 2810
BseNI ACTGG 6 cut(s) 34, 104, 131, 202, 1470, 2044
BseSI GKGCMC 2 cut(s) 1121, 2092
BseXI GCAGC 4 cut(s) 20, 280, 1836, 2236
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 432, 3123
BshFI GGCC 7 cut(s) 546, 918, 1011, 1119, 2205, 3039, 3182
BshNI GGYRCC 1 cut(s) 200
BshVI ATCGAT 2 cut(s) 963, 2213
BsiHKAI GWGCWC 4 cut(s) 2092, 2104, 2196, 3056
BsiHKCI CYCGRG 1 cut(s) 2860
BsiSI CCGG 2 cut(s) 567, 3113
BslFI GGGAC 3 cut(s) 859, 908, 3040
BslI CCNNNNNNNGG 4 cut(s) 218, 697, 2042, 2840
BsmAI GTCTC 4 cut(s) 998, 1855, 1974, 2863
BsmFI GGGAC 3 cut(s) 859, 908, 3040
BsmI GAATGC 1 cut(s) 1430
BsnI GGCC 7 cut(s) 546, 918, 1011, 1119, 2205, 3039, 3182
Bso31I GGTCTC 1 cut(s) 1855
BsoBI CYCGRG 1 cut(s) 2860
Bsp120I GGGCCC 1 cut(s) 1117
Bsp1286I GDGCHC 5 cut(s) 1121, 2092, 2104, 2196, 3056
Bsp1407I TGTACA 1 cut(s) 2877
Bsp143I GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 7 cut(s) 144, 301, 1062, 1407, 2408, 2541, 3059
BspANI GGCC 7 cut(s) 546, 918, 1011, 1119, 2205, 3039, 3182
BspCNI CTCAG 9 cut(s) 91, 480, 591, 998, 1269, 1434, 1760, 2366, 2809
BspDI ATCGAT 2 cut(s) 963, 2213
BspLI GGNNCC 6 cut(s) 202, 367, 633, 702, 1119, 2832
BspMAI CTGCAG 3 cut(s) 10, 2619, 3190
BspMI ACCTGC 1 cut(s) 2129
BspPI GGATC 3 cut(s) 1030, 1063, 1456
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 200
BspTI CTTAAG 2 cut(s) 1369, 1402
BspTNI GGTCTC 1 cut(s) 1855
BsrDI GCAATG 2 cut(s) 409, 2969
BsrFI RCCGGY 1 cut(s) 566
BsrGI TGTACA 1 cut(s) 2877
BsrI ACTGG 6 cut(s) 34, 104, 131, 202, 1470, 2044
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 8 cut(s) 268, 280, 281, 871, 1282, 1318, 1486, 1860
BssMI GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
BssNAI GTATAC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 5 cut(s) 268, 871, 1318, 1486, 1860
Bst1107I GTATAC 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 857
Bst2UI CCWGG 3 cut(s) 282, 1284, 1427
Bst4CI ACNGT 6 cut(s) 70, 430, 617, 1946, 2371, 3209
Bst6I CTCTTC 4 cut(s) 48, 2068, 2860, 3111
BstAFI CTTAAG 2 cut(s) 1369, 1402
BstAPI GCANNNNNTGC 1 cut(s) 2747
BstAUI TGTACA 1 cut(s) 2877
BstC8I GCNNGC 1 cut(s) 3097
BstDSI CCRYGG 1 cut(s) 268
BstEII GGTNACC 2 cut(s) 866, 1613
BstF5I GGATG 7 cut(s) 104, 249, 1275, 1367, 1721, 2878, 3071
BstHHI GCGC 2 cut(s) 13, 249
BstKTI GATC 7 cut(s) 90, 466, 810, 1038, 1071, 1451, 1628
BstMAI GTCTC 4 cut(s) 998, 1855, 1974, 2863
BstMBI GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
BstMWI GCNNNNNNNGC 7 cut(s) 47, 56, 150, 811, 1404, 2747, 3101
BstNI CCWGG 3 cut(s) 282, 1284, 1427
BstNSI RCATGY 4 cut(s) 144, 583, 2560, 2747
BstPI GGTNACC 2 cut(s) 866, 1613
BstSCI CCNGG 3 cut(s) 280, 1282, 1425
BstSFI CTRYAG 3 cut(s) 6, 2615, 3186
BstSLI GKGCMC 2 cut(s) 1121, 2092
BstV1I GCAGC 4 cut(s) 20, 280, 1836, 2236
BstV2I GAAGAC 2 cut(s) 565, 576
BstX2I RGATCY 3 cut(s) 87, 1035, 1068
BstXI CCANNNNNNTGG 1 cut(s) 3023
BstYI RGATCY 3 cut(s) 87, 1035, 1068
BstZ17I GTATAC 1 cut(s) 779
Bsu15I ATCGAT 2 cut(s) 963, 2213
BsuI GTATCC 1 cut(s) 1643
BsuRI GGCC 7 cut(s) 546, 918, 1011, 1119, 2205, 3039, 3182
BsuTUI ATCGAT 2 cut(s) 963, 2213
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 7 cut(s) 104, 249, 1275, 1367, 1721, 2878, 3071
BtsI GCAGTG 2 cut(s) 731, 3183
BtsIMutI CAGTG 5 cut(s) 195, 731, 775, 1463, 3183
BveI ACCTGC 1 cut(s) 2129
Cac8I GCNNGC 1 cut(s) 3097
CfoI GCGC 2 cut(s) 13, 249
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 6 cut(s) 366, 701, 1117, 1118, 1739, 2045
ClaI ATCGAT 2 cut(s) 963, 2213
CseI GACGC 1 cut(s) 811
Csp6I GTAC 7 cut(s) 1423, 1593, 2546, 2554, 2659, 2878, 2894
CspCI CAANNNNNGTGG 4 cut(s) 628, 663, 751, 786
CviQI GTAC 7 cut(s) 1423, 1593, 2546, 2554, 2659, 2878, 2894
DpnI GATC 7 cut(s) 89, 465, 809, 1037, 1070, 1450, 1627
DpnII GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
DraI TTTAAA 1 cut(s) 1296
DraIII CACNNNGTG 2 cut(s) 899, 2096
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 3180
Eam1104I CTCTTC 4 cut(s) 48, 2068, 2860, 3111
EarI CTCTTC 4 cut(s) 48, 2068, 2860, 3111
EciI GGCGGA 1 cut(s) 3074
Eco130I CCWWGG 5 cut(s) 268, 871, 1318, 1486, 1860
Eco147I AGGCCT 3 cut(s) 918, 1011, 2205
Eco24I GRGCYC 1 cut(s) 1121
Eco31I GGTCTC 1 cut(s) 1855
Eco47I GGWCC 4 cut(s) 366, 701, 1739, 2045
Eco57I CTGAAG 2 cut(s) 7, 2570
Eco88I CYCGRG 1 cut(s) 2860
Eco91I GGTNACC 2 cut(s) 866, 1613
EcoO109I RGGNCCY 1 cut(s) 366
EcoO65I GGTNACC 2 cut(s) 866, 1613
EcoRII CCWGG 3 cut(s) 280, 1282, 1425
EcoT14I CCWWGG 5 cut(s) 268, 871, 1318, 1486, 1860
EcoT38I GRGCYC 1 cut(s) 1121
ErhI CCWWGG 5 cut(s) 268, 871, 1318, 1486, 1860
FaqI GGGAC 3 cut(s) 859, 908, 3040
FblI GTMKAC 3 cut(s) 72, 778, 1246
Fnu4HI GCNGC 8 cut(s) 6, 9, 294, 302, 1408, 1825, 2250, 2541
FokI GGATG 7 cut(s) 91, 256, 1282, 1374, 1708, 2885, 3058
FriOI GRGCYC 1 cut(s) 1121
Fsp4HI GCNGC 8 cut(s) 6, 9, 294, 302, 1408, 1825, 2250, 2541
GlaI GCGC 2 cut(s) 12, 248
GluI GCNGC 8 cut(s) 6, 9, 294, 302, 1408, 1825, 2250, 2541
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 3 cut(s) 195, 1974, 2817
HaeIII GGCC 7 cut(s) 546, 918, 1011, 1119, 2205, 3039, 3182
HapII CCGG 2 cut(s) 567, 3113
HgaI GACGC 1 cut(s) 811
HhaI GCGC 2 cut(s) 13, 249
Hin6I GCGC 2 cut(s) 11, 247
HinP1I GCGC 2 cut(s) 11, 247
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 2 cut(s) 715, 2768
HpaII CCGG 2 cut(s) 567, 3113
HphI GGTGA 5 cut(s) 58, 158, 860, 2680, 3102
Hpy99I CGWCG 2 cut(s) 74, 119
HpyAV CCTTC 9 cut(s) 545, 1611, 1629, 1686, 1874, 2429, 2672, 2843, 3029
HpyCH4III ACNGT 6 cut(s) 70, 430, 617, 1946, 2371, 3209
HpyCH4IV ACGT 4 cut(s) 117, 311, 1233, 2384
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 56, 150, 811, 1404, 2747, 3101
HpySE526I ACGT 4 cut(s) 117, 311, 1233, 2384
HspAI GCGC 2 cut(s) 11, 247
Kzo9I GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 2 cut(s) 2099, 2836
Lsp1109I GCAGC 4 cut(s) 20, 280, 1836, 2236
LweI GCATC 8 cut(s) 234, 823, 1352, 2078, 2219, 2654, 3001, 3127
MaeII ACGT 4 cut(s) 117, 311, 1233, 2384
MaeIII GTNAC 5 cut(s) 136, 866, 1376, 1613, 2268
MalI GATC 7 cut(s) 89, 465, 809, 1037, 1070, 1450, 1627
MboI GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
MfeI CAATTG 1 cut(s) 2108
MflI RGATCY 3 cut(s) 87, 1035, 1068
MhlI GDGCHC 5 cut(s) 1121, 2092, 2104, 2196, 3056
MlsI TGGCCA 1 cut(s) 3182
MluNI TGGCCA 1 cut(s) 3182
MlyI GAGTC 8 cut(s) 171, 300, 610, 1965, 2072, 2578, 2788, 3022
MmeI TCCRAC 6 cut(s) 132, 138, 1481, 1962, 2060, 2634
Mox20I TGGCCA 1 cut(s) 3182
MroXI GAANNNNTTC 1 cut(s) 2293
MscI TGGCCA 1 cut(s) 3182
MslI CAYNNNNRTG 1 cut(s) 2673
Msp20I TGGCCA 1 cut(s) 3182
MspCI CTTAAG 2 cut(s) 1369, 1402
MspI CCGG 2 cut(s) 567, 3113
MspR9I CCNGG 3 cut(s) 282, 1284, 1427
MunI CAATTG 1 cut(s) 2108
Mva1269I GAATGC 1 cut(s) 1430
MvaI CCWGG 3 cut(s) 282, 1284, 1427
MwoI GCNNNNNNNGC 7 cut(s) 47, 56, 150, 811, 1404, 2747, 3101
NcoI CCATGG 1 cut(s) 268
NdeII GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
NlaIV GGNNCC 6 cut(s) 202, 367, 633, 702, 1119, 2832
NmuCI GTSAC 1 cut(s) 866
NspI RCATGY 4 cut(s) 144, 583, 2560, 2747
PaeR7I CTCGAG 1 cut(s) 2860
PaqCI CACCTGC 1 cut(s) 2129
PasI CCCWGGG 1 cut(s) 281
PceI AGGCCT 3 cut(s) 918, 1011, 2205
PciI ACATGT 2 cut(s) 579, 2743
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 1430
PdmI GAANNNNTTC 1 cut(s) 2293
PfeI GAWTC 9 cut(s) 327, 349, 652, 960, 976, 1324, 1480, 2786, 3107
PflMI CCANNNNNTGG 2 cut(s) 2042, 2840
PkrI GCNGC 8 cut(s) 7, 10, 295, 303, 1409, 1826, 2251, 2542
PleI GAGTC 8 cut(s) 170, 300, 609, 1964, 2072, 2577, 2788, 3022
PpsI GAGTC 8 cut(s) 170, 300, 609, 1964, 2072, 2577, 2788, 3022
PpuMI RGGWCCY 1 cut(s) 366
PscI ACATGT 2 cut(s) 579, 2743
PshBI ATTAAT 1 cut(s) 1578
PsiI TTATAA 1 cut(s) 3218
Psp1406I AACGTT 2 cut(s) 1233, 2384
Psp5II RGGWCCY 1 cut(s) 366
Psp6I CCWGG 3 cut(s) 280, 1282, 1425
PspEI GGTNACC 2 cut(s) 866, 1613
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 280, 1282, 1425
PspN4I GGNNCC 6 cut(s) 202, 367, 633, 702, 1119, 2832
PspOMI GGGCCC 1 cut(s) 1117
PspPI GGNCC 6 cut(s) 366, 701, 1117, 1118, 1739, 2045
PspPPI RGGWCCY 1 cut(s) 366
PstI CTGCAG 3 cut(s) 10, 2619, 3190
PsuI RGATCY 3 cut(s) 87, 1035, 1068
RsaI GTAC 7 cut(s) 1424, 1594, 2547, 2555, 2660, 2879, 2895
RsaNI GTAC 7 cut(s) 1423, 1593, 2546, 2554, 2659, 2878, 2894
RseI CAYNNNNRTG 1 cut(s) 2673
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 8 cut(s) 6, 9, 294, 302, 1408, 1825, 2250, 2541
Sau3AI GATC 7 cut(s) 87, 463, 807, 1035, 1068, 1448, 1625
Sau96I GGNCC 6 cut(s) 366, 701, 1117, 1118, 1739, 2045
ScaI AGTACT 1 cut(s) 2547
SchI GAGTC 8 cut(s) 171, 300, 610, 1965, 2072, 2578, 2788, 3022
ScrFI CCNGG 3 cut(s) 282, 1284, 1427
SduI GDGCHC 5 cut(s) 1121, 2092, 2104, 2196, 3056
SfaNI GCATC 8 cut(s) 234, 823, 1352, 2078, 2219, 2654, 3001, 3127
SfcI CTRYAG 3 cut(s) 6, 2615, 3186
Sfr274I CTCGAG 1 cut(s) 2860
SinI GGWCC 4 cut(s) 366, 701, 1739, 2045
SlaI CTCGAG 1 cut(s) 2860
SmiMI CAYNNNNRTG 1 cut(s) 2673
SmlI CTYRAG 4 cut(s) 1369, 1402, 2860, 3030
SmoI CTYRAG 4 cut(s) 1369, 1402, 2860, 3030
SseBI AGGCCT 3 cut(s) 918, 1011, 2205
SsiI CCGC 7 cut(s) 144, 301, 1062, 1407, 2408, 2541, 3059
SspI AATATT 1 cut(s) 790
StuI AGGCCT 3 cut(s) 918, 1011, 2205
StyD4I CCNGG 3 cut(s) 280, 1282, 1425
StyI CCWWGG 5 cut(s) 268, 871, 1318, 1486, 1860
TaaI ACNGT 6 cut(s) 70, 430, 617, 1946, 2371, 3209
TaiI ACGT 4 cut(s) 120, 314, 1236, 2387
TaqI TCGA 6 cut(s) 72, 610, 963, 2213, 2491, 2861
TatI WGTACW 4 cut(s) 2545, 2553, 2877, 2893
TauI GCSGC 3 cut(s) 304, 1410, 2543
TfiI GAWTC 9 cut(s) 327, 349, 652, 960, 976, 1324, 1480, 2786, 3107
TscAI CASTG 5 cut(s) 202, 731, 775, 1470, 3190
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 5 cut(s) 5, 8, 293, 1824, 2249
Tsp45I GTSAC 1 cut(s) 866
TspGWI ACGGA 1 cut(s) 1492
TspRI CASTG 5 cut(s) 202, 731, 775, 1470, 3190
Van91I CCANNNNNTGG 2 cut(s) 2042, 2840
Vha464I CTTAAG 2 cut(s) 1369, 1402
VneI GTGCAC 1 cut(s) 2088
VpaK11BI GGWCC 4 cut(s) 366, 701, 1739, 2045
VspI ATTAAT 1 cut(s) 1578
XapI RAATTY 8 cut(s) 676, 1076, 1261, 1642, 1843, 2002, 2031, 2974
XbaI TCTAGA 2 cut(s) 1972, 2986
XceI RCATGY 4 cut(s) 144, 583, 2560, 2747
XcmI CCANNNNNNNNNTGG 3 cut(s) 1173, 2824, 3175
XhoI CTCGAG 1 cut(s) 2860
XmiI GTMKAC 3 cut(s) 72, 778, 1246
XmnI GAANNNNTTC 1 cut(s) 2293
ZrmI AGTACT 1 cut(s) 2547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.