RchiOBHm_Chr2g0114381

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000058
Physical Location & Seq
Reverse (-)
26362444 .. 26369090
6647 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48761

Sequence Viewer

Length: 3600 bp
ATGGAGAGAGTTACTTGGTTCTTCTTGGTATACTTGATCTGTTGTGAGGCGGCTAGCTGCTTGACTATGGCAGCACAAACCAACCTTAACATCACCACAGACCGGTCTGCTCTTCTTGCTCTAAAAGACCATATCACCAGTGACCCTCAAAATATGATCCTCACCAATTGGTCAACCACCACCCCTATTTGCAACTGGGTTGGCGTCATTTGTGGTGCACGCCATCATCGAGTCGCAGCGTTGAACCTCTCTTACTTTGGTCTCGCGGGCACCATTCCTCCTGAGCTAGGCAACTTATCATTGCTTGTTGATATGGACATCCAAAATAACAGTTTCCAGGGTACCTTGCCTGACGAGTTGACTCGCCTGCGCCGGTTGAAGTTCATTAACTTGGGATACAACAAGTTTATGGGAGTGATACCGTCATGGTTTGGGTCCTTGTCCAAACTTCAAGGATTCATGTTGTTTGGTAATCAATTTGCAAGTTCTATACCAACTGCCATCTTCAACTTATCTGCTCTTCAAATATTTAATCTGAGTCATAACCAACTCTCAGAGACCGGACGACGCCGCAGAGGTCGGACGACGCCTCAGAGGCCGCATATCGATCTACAGGCCGGACGACGCTGCATAGGCCGAAGACGACGCCTCAGAGGCCGCATATCGAGGACTCTGTTGAGATCGGACAGTGATGAGCACGGTCGATTTCGGCGACCGGGCTTCTGGTCGATTTCGGCGATCTGGGCTTTCCGATCTGCGGCGCCGATTGCAGAAGCAGCATCCACTCCTGAAGACGACAAAGCAGCTTTGGTCAGCCTACCTCGCCGGAGTCCAACACCTGGTAGGCAAGAAACACAAATTCCAAATGAGATCGGCACCTTGAATCAGCTTGAAGAGTTGTTTGTGCAAAATAATGCCTTAAAAGGGCATATTCCTATGGTCGTCTTCAACATGTCATCTTTGACTAGAGTAAATTTCTATGGAAACAAGTTGAATGGTAGAATTCCCGACAATATATGTCAACATCTTCCAAATATCCAAGAGTTGAATTTGGGTAGCAATCAGTTTGATGGTCCACTTCCATCCAAGTTATGGCAATGCAAACAACTTCTTAAATTACATTTGGGGGATAACAAGTTCAGTGGAAGAATACCCAAAGATATTGGGAACTTAACTCGGATAAAGAAGATTGGGTTTTACACCAACAGCTTAACAGGTACTATACCATATGAGTTTGGTAATCTTCCAAATTTAGAGTGGTTGGTGCTCCAGGAGAATAAACTAAATGGCCATATCCCATCGTCAATCTTCAATATGTCCACAATAACATTATTAAATCTCAACATCAATCAGCTTTCAGGAAGCCTCCCAACAAACATAGGCCTTGCGCTTCCAAACCTAGAATCGCTTGCAGTAGCAGCGAATCACCTCAGTGGAGTAATCCCCAAATTCATCTCCAATGCTTCTGAGCTTTGGCTTCTAGAACTGGGAGGTAACTCTTTTTCTGGATTTATTCCAAGCACTCTGTGCCTCTTGACAAACCTTGAATGGCTAGGATTGTCCGAGAATAATTTGACCATTGATACCTCTACTCCAGAAGTTAATATTTTGTCTTGTCTGCCAAATCTTAGAAATTTGAGGTGGTTAAACTTCGAAAACAATCCATTAAATGCGAAGCTTTTGATTCCCTTCAGTAATATCTCTGCATCACTTCAAAAACTTTATTTAGAAAATTGCAGCTTGAGAGGTAACATCCCCAGTGATATTGGGAATTTGAGTAGCTTGATACTTTTAGGCCTCCAAGACAATCAATTGAGCGGGCCAATTCCAAGCTCAATGGGAAGACTACATAATCTTCAAGCTTTGGGTATGTATGATAACACATTGCAAGGATACATTCCAGATGAGCTTTGTCAACTAGAAAACTTATTTGTTTTGTACTTGGGTGGGAATCAACTCTCTGGTTCTGTACCTTCCTGCTTGGGTAATCTAACAGCATCTCTAAGAGGACTATCCTTAGGGTCCAATTTGTTGAATTCCACAATCCCATCTTCCTTGTGGGGACTTACAGATATCTTGGGACTAGACTTGTCATCCAATTCTCTAATTGGACCTCTATCAGGTGCTGTTGGTAATTTGAAAGCTGCTATATACATAAATGTATCATACAACCAATTATCTGGGAGTGTACCAAGTAGCATTGGGAGTTTAGTGAGTTTGGTCAATCTCTCCTTAGCACATAACAATCTAGAAGGCCCTATTCCTAGTTCCTTTGCAACTTGTGTAAGCTTAGAAGGATTGGATTTATCTCGAAATAGTTTCACTGGAGTGATTCCAAAGTCTCTAGAAGCACTCTTATATCTCAAGCATCTGAATTTGTCTTTCAACAGACTCCAAGGAGAAATTCCAAGCGGCGGTCCTTTCAAAAACTTCTCTGCTGAATCATTTGTTTCAAACTATGGACTCTGTGGTGGATCCCAATTTCAAGTTCCACCATGCAAAAGAAAAACTAGTATATCTATCTTGAAATCTATTATTCCGGGGATCTTGTCAGCAACGCTTCTAGCGGCCTCCATAATTTGGATGTTGATGTTACGCAGAAAAAAGAATGCAACAGCTGCAGCACATATTATCTTGATGCCTCAAGTTTTATGCAGAAGGGTTTCATACCAAGAGCTCCTGTGTGCGACAGATGGATTCAATGAAAGCAACTTACTTGGTATTGGGGGTTTTGGCTCAGTATATAAAGGAACATTTCCAGATGAGACAGATGTTGCAATAAAGGTTTTCAATTTGGATTTAGAAGGGGCATTTGCAAGTTTTGAGGTTGAATGTGAAATGCTAAGTAATGTTCGTCACAGAAATCTTGTCAAAGTCATCAGTTGTTGTAGTCAAATTGATTTCAAAGGTCTTGTATTGAATTACATGCCTAATGGGAGCCTTGAGAAGTGGTTGTATTCTCAGAATTCTTCTTTGGACTTAGTGCAGAGGTTGGATATAATGATAGATGTTGCATTGGCATTGGAGTACCTTCACCATGATTATGAAGTACCTATTGTCCATTGCGATTTGAAGCCGAGCAACATCTTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGTATTGCAAAACTCTTGGGTGGAGGAGATTCTATGACTCAAACCATGACCCTGGCCACAATTGGGTATATTGCACCAGAGTATGGAACGGAAGGAATAGTGACCAGAAGAGGGGATGTGTATAGTTTCGGTATTGTGGTGATGGAAACATTTACAAGAAGGAAACCAACTGATGAGATCTTCACTGGGGAAATGAGAATAAGTCAATGGGTTGCAAATCACCTTGTTGCAAATGCGATAGTTGATGTTGTGGATGCTACTTTACTTGGAACCGAGGAGGATCATGAGTTCGTGAGCAAGAGGGAGTGCTTATCATCTATTATGAGATTAGCTGTTGCTTGTTCAACAGAATCACTGGAAGAGAGGATAAGCATGCAAGAGGCTGTAGCCATGCTTAAAACAATCAAGATCAAGTTTTCAAAGCAAAGTACTGCTGCAGGAGGTGTGGTGTTGAACTATCGTCCTGTTTGGTAA

Protein Analysis

1199

Amino Acids

131.74

Weight (kDa)

7.5

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 2.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 81 - 182 9.5e-06 Leucine-rich repeat region
LRR_8 PF13855 465 - 525 3.8e-07 Leucine rich repeat
LRR_14 PF23598 571 - 676 1e-08 Leucine-rich repeat region
LRR_8 PF13855 755 - 798 1e-05 Leucine rich repeat
Pkinase PF00069 901 - 1172 9.1e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 904 - 1172 1.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 4 cut(s) 269, 341, 760, 875
AccB7I CCANNNNNTGG 4 cut(s) 839, 1092, 2580, 3209
AccBSI CCGCTC 1 cut(s) 1818
AccI GTMKAC 1 cut(s) 30
AccII CGCG 1 cut(s) 266
AclWI GGATC 5 cut(s) 151, 2469, 2482, 2552, 3414
AcoI YGGCCR 2 cut(s) 1288, 3180
AcuI CTGAAG 2 cut(s) 810, 1675
AcyI GRCGYC 5 cut(s) 204, 568, 587, 646, 761
AdeI CACNNNGTG 1 cut(s) 1527
AfaI GTAC 8 cut(s) 343, 1219, 1940, 1971, 2190, 3029, 3051, 3556
AflIII ACRYGT 2 cut(s) 951, 3112
AgeI ACCGGT 1 cut(s) 102
AhlI ACTAGT 1 cut(s) 2510
AjnI CCWGG 4 cut(s) 336, 838, 1269, 3177
AjuI GAANNNNNNNTTGG 6 cut(s) 2388, 2420, 2472, 2504, 2957, 2989
AleI CACNNNNGTG 2 cut(s) 1431, 2327
AloI GAACNNNNNNTCC 4 cut(s) 1121, 1153, 3398, 3430
Alw21I GWGCWC 4 cut(s) 220, 699, 1269, 2679
Alw26I GTCTC 4 cut(s) 266, 551, 2346, 2759
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 5 cut(s) 151, 2469, 2482, 2552, 3414
AlwNI CAGNNNCTG 1 cut(s) 2126
ApaLI GTGCAC 1 cut(s) 216
AsiGI ACCGGT 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 2662
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 3 cut(s) 372, 763, 1390
AspS9I GGNCC 7 cut(s) 435, 1073, 1820, 2022, 2111, 2255, 2417
AsuC2I CCSGG 2 cut(s) 717, 2541
AsuHPI GGTGA 7 cut(s) 85, 127, 154, 1418, 3026, 3277, 3338
AsuII TTCGAA 1 cut(s) 1653
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 5 cut(s) 435, 1073, 2022, 2111, 2417
AxyI CCTNAGG 1 cut(s) 2017
BaeGI GKGCMC 2 cut(s) 220, 272
BalI TGGCCA 2 cut(s) 1290, 3182
BamHI GGATCC 1 cut(s) 2474
BanI GGYRCC 4 cut(s) 269, 341, 760, 875
BanII GRGCYC 1 cut(s) 2679
BbsI GAAGAC 4 cut(s) 646, 798, 937, 1849
Bbv12I GWGCWC 4 cut(s) 220, 699, 1269, 2679
BccI CCATC 8 cut(s) 231, 509, 1064, 1090, 1306, 2056, 2687, 3262
BciT130I CCWGG 4 cut(s) 338, 840, 1271, 3179
BciVI GTATCC 2 cut(s) 389, 1886
BcnI CCSGG 2 cut(s) 717, 2541
BcoDI GTCTC 4 cut(s) 266, 551, 2346, 2759
BcuI ACTAGT 1 cut(s) 2510
BfmI CTRYAG 4 cut(s) 611, 2619, 3510, 3561
BfoI RGCGCY 1 cut(s) 764
BfuI GTATCC 2 cut(s) 389, 1886
BglI GCCNNNNNGGC 2 cut(s) 595, 654
BglII AGATCT 1 cut(s) 3303
BmcAI AGTACT 1 cut(s) 3556
Bme1390I CCNGG 6 cut(s) 338, 717, 840, 1271, 2541, 3179
Bme18I GGWCC 5 cut(s) 435, 1073, 2022, 2111, 2417
BmgT120I GGNCC 7 cut(s) 435, 1073, 1820, 2022, 2111, 2255, 2417
BmiI GGNNCC 9 cut(s) 271, 343, 436, 762, 877, 2023, 2476, 2939, 3397
BmrFI CCNGG 6 cut(s) 338, 717, 840, 1271, 2541, 3179
BmrI ACTGGG 4 cut(s) 205, 1496, 1752, 3321
BmsI GCATC 6 cut(s) 788, 1715, 2006, 2377, 2628, 3370
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 4 cut(s) 205, 1496, 1752, 3321
BpiI GAAGAC 4 cut(s) 646, 798, 937, 1849
BpmI CTGGAG 3 cut(s) 1253, 1578, 2346
Bpu10I CCTNAGC 2 cut(s) 282, 2233
Bpu14I TTCGAA 1 cut(s) 1653
BpuEI CTTGAG 4 cut(s) 1762, 2348, 2628, 2963
BpuMI CCSGG 2 cut(s) 717, 2541
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 5 cut(s) 204, 568, 587, 646, 761
BsaI GGTCTC 2 cut(s) 266, 551
BsaJI CCNNGG 5 cut(s) 337, 2395, 2540, 3177, 3399
BsaWI WCCGGW 2 cut(s) 102, 560
BsaXI ACNNNNNCTCC 6 cut(s) 262, 292, 1576, 1606, 3558, 3588
Bse118I RCCGGY 2 cut(s) 102, 372
Bse1I ACTGG 7 cut(s) 138, 200, 1491, 1758, 2329, 3316, 3486
Bse21I CCTNAGG 1 cut(s) 2017
Bse3DI GCAATG 4 cut(s) 299, 1103, 1883, 3061
BseBI CCWGG 4 cut(s) 338, 840, 1271, 3179
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 5 cut(s) 337, 2395, 2540, 3177, 3399
BseGI GGATG 8 cut(s) 318, 779, 1082, 1752, 2093, 2589, 3247, 3385
BseMI GCAATG 4 cut(s) 299, 1103, 1883, 3061
BseMII CTCAG 9 cut(s) 273, 527, 567, 605, 664, 1444, 1458, 2751, 2975
BseNI ACTGG 7 cut(s) 138, 200, 1491, 1758, 2329, 3316, 3486
BseRI GAGGAG 2 cut(s) 3165, 3416
BseSI GKGCMC 2 cut(s) 220, 272
BsgI GTGCAG 1 cut(s) 3005
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 2 cut(s) 703, 716
BshNI GGYRCC 4 cut(s) 269, 341, 760, 875
BshTI ACCGGT 1 cut(s) 102
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 2 cut(s) 703, 716
BsiHKAI GWGCWC 4 cut(s) 220, 699, 1269, 2679
BsiSI CCGG 7 cut(s) 103, 373, 561, 618, 716, 826, 2540
BslFI GGGAC 2 cut(s) 2076, 2094
BsmAI GTCTC 4 cut(s) 266, 551, 2346, 2759
BsmFI GGGAC 2 cut(s) 2076, 2094
BsmI GAATGC 1 cut(s) 2614
Bso31I GGTCTC 2 cut(s) 266, 551
Bsp119I TTCGAA 1 cut(s) 1653
Bsp1286I GDGCHC 5 cut(s) 220, 272, 699, 1269, 2679
BspCNI CTCAG 9 cut(s) 274, 528, 566, 604, 663, 1443, 1459, 2750, 2974
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 3409
BspLI GGNNCC 9 cut(s) 271, 343, 436, 762, 877, 2023, 2476, 2939, 3397
BspMAI CTGCAG 2 cut(s) 2623, 3565
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 5 cut(s) 151, 2469, 2482, 2552, 3414
BspQI GCTCTTC 2 cut(s) 117, 525
BspT104I TTCGAA 1 cut(s) 1653
BspT107I GGYRCC 4 cut(s) 269, 341, 760, 875
BspTNI GGTCTC 2 cut(s) 266, 551
BsrBI CCGCTC 1 cut(s) 1818
BsrDI GCAATG 4 cut(s) 299, 1103, 1883, 3061
BsrFI RCCGGY 2 cut(s) 102, 372
BsrI ACTGG 7 cut(s) 138, 200, 1491, 1758, 2329, 3316, 3486
BssAI RCCGGY 2 cut(s) 102, 372
BssECI CCNNGG 5 cut(s) 337, 2395, 2540, 3177, 3399
BssNAI GTATAC 1 cut(s) 31
BssNI GRCGYC 5 cut(s) 204, 568, 587, 646, 761
BssT1I CCWWGG 1 cut(s) 2395
Bst1107I GTATAC 1 cut(s) 31
Bst2UI CCWGG 4 cut(s) 338, 840, 1271, 3179
Bst4CI ACNGT 4 cut(s) 332, 423, 689, 701
Bst6I CTCTTC 5 cut(s) 117, 525, 888, 3229, 3480
BstACI GRCGYC 5 cut(s) 204, 568, 587, 646, 761
BstAPI GCANNNNNTGC 3 cut(s) 1527, 2618, 3116
BstBI TTCGAA 1 cut(s) 1653
BstC8I GCNNGC 7 cut(s) 55, 220, 268, 368, 1410, 1820, 3500
BstENI CCTNNNNNAGG 2 cut(s) 285, 2118
BstF5I GGATG 8 cut(s) 318, 779, 1082, 1752, 2093, 2589, 3247, 3385
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 3 cut(s) 372, 763, 1390
BstMAI GTCTC 4 cut(s) 266, 551, 2346, 2759
BstMCI CGRYCG 2 cut(s) 703, 716
BstNI CCWGG 4 cut(s) 338, 840, 1271, 3179
BstNSI RCATGY 4 cut(s) 955, 2929, 3116, 3502
BstSCI CCNGG 6 cut(s) 336, 715, 838, 1269, 2539, 3177
BstSFI CTRYAG 4 cut(s) 611, 2619, 3510, 3561
BstSLI GKGCMC 2 cut(s) 220, 272
BstUI CGCG 1 cut(s) 266
BstV2I GAAGAC 4 cut(s) 646, 798, 937, 1849
BstX2I RGATCY 3 cut(s) 2474, 2544, 3303
BstXI CCANNNNNNTGG 2 cut(s) 2180, 3178
BstYI RGATCY 3 cut(s) 2474, 2544, 3303
BstZ17I GTATAC 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 606
Bsu36I CCTNAGG 1 cut(s) 2017
BsuI GTATCC 2 cut(s) 389, 1886
BsuTUI ATCGAT 1 cut(s) 606
BtsCI GGATG 8 cut(s) 318, 779, 1082, 1752, 2093, 2589, 3247, 3385
BtsIMutI CAGTG 8 cut(s) 145, 694, 1147, 1438, 1765, 2322, 3309, 3479
Cac8I GCNNGC 7 cut(s) 55, 220, 268, 368, 1410, 1820, 3500
CaiI CAGNNNCTG 1 cut(s) 2126
CciI TCATGA 1 cut(s) 3409
CfoI GCGC 3 cut(s) 372, 763, 1390
Cfr10I RCCGGY 2 cut(s) 102, 372
Cfr13I GGNCC 7 cut(s) 435, 1073, 1820, 2022, 2111, 2255, 2417
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 5 cut(s) 193, 576, 595, 633, 654
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 8 cut(s) 342, 1218, 1939, 1970, 2189, 3028, 3050, 3555
CspAI ACCGGT 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 1123, 1158
CviQI GTAC 8 cut(s) 342, 1218, 1939, 1970, 2189, 3028, 3050, 3555
DinI GGCGCC 1 cut(s) 762
DraIII CACNNNGTG 1 cut(s) 1527
EaeI YGGCCR 2 cut(s) 1288, 3180
Eam1104I CTCTTC 5 cut(s) 117, 525, 888, 3229, 3480
EarI CTCTTC 5 cut(s) 117, 525, 888, 3229, 3480
Ecl136II GAGCTC 1 cut(s) 2677
Eco130I CCWWGG 1 cut(s) 2395
Eco147I AGGCCT 2 cut(s) 1383, 1797
Eco24I GRGCYC 1 cut(s) 2679
Eco31I GGTCTC 2 cut(s) 266, 551
Eco32I GATATC 1 cut(s) 2074
Eco47I GGWCC 5 cut(s) 435, 1073, 2022, 2111, 2417
Eco53kI GAGCTC 1 cut(s) 2677
Eco57I CTGAAG 2 cut(s) 810, 1675
Eco81I CCTNAGG 1 cut(s) 2017
EcoICRI GAGCTC 1 cut(s) 2677
EcoNI CCTNNNNNAGG 2 cut(s) 285, 2118
EcoO109I RGGNCCY 2 cut(s) 435, 2255
EcoRI GAATTC 3 cut(s) 1002, 2035, 2965
EcoRII CCWGG 4 cut(s) 336, 838, 1269, 3177
EcoRV GATATC 1 cut(s) 2074
EcoT14I CCWWGG 1 cut(s) 2395
EcoT38I GRGCYC 1 cut(s) 2679
EgeI GGCGCC 1 cut(s) 762
EheI GGCGCC 1 cut(s) 762
ErhI CCWWGG 1 cut(s) 2395
FaqI GGGAC 2 cut(s) 2076, 2094
FauI CCCGC 2 cut(s) 259, 1811
FauNDI CATATG 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 30
FokI GGATG 8 cut(s) 305, 766, 1069, 1739, 2080, 2596, 3254, 3392
FriOI GRGCYC 1 cut(s) 2679
GlaI GCGC 3 cut(s) 371, 762, 1389
GsuI CTGGAG 3 cut(s) 1253, 1578, 2346
HaeII RGCGCY 1 cut(s) 764
HapII CCGG 7 cut(s) 103, 373, 561, 618, 716, 826, 2540
HgaI GACGC 5 cut(s) 193, 576, 595, 633, 654
HhaI GCGC 3 cut(s) 372, 763, 1390
Hin1I GRCGYC 5 cut(s) 204, 568, 587, 646, 761
Hin6I GCGC 3 cut(s) 370, 761, 1388
HinP1I GCGC 3 cut(s) 370, 761, 1388
HincII GTYRAC 4 cut(s) 174, 360, 1022, 1916
HindII GTYRAC 4 cut(s) 174, 360, 1022, 1916
HindIII AAGCTT 3 cut(s) 1676, 1860, 2287
HpaII CCGG 7 cut(s) 103, 373, 561, 618, 716, 826, 2540
HphI GGTGA 7 cut(s) 85, 127, 154, 1418, 3026, 3277, 3338
Hpy166II GTNNAC 9 cut(s) 31, 174, 218, 360, 1022, 1076, 1320, 1916, 2189
Hpy8I GTNNAC 9 cut(s) 31, 174, 218, 360, 1022, 1076, 1320, 1916, 2189
Hpy99I CGWCG 4 cut(s) 570, 589, 627, 648
HpyAV CCTTC 9 cut(s) 1699, 1983, 2246, 2288, 2652, 2798, 3041, 3212, 3279
HpyCH4III ACNGT 4 cut(s) 332, 423, 689, 701
Hsp92I GRCGYC 5 cut(s) 204, 568, 587, 646, 761
HspAI GCGC 3 cut(s) 370, 761, 1388
KasI GGCGCC 1 cut(s) 760
KpnI GGTACC 1 cut(s) 345
LguI GCTCTTC 2 cut(s) 117, 525
LmnI GCTCC 3 cut(s) 1272, 2682, 2937
LweI GCATC 6 cut(s) 788, 1715, 2006, 2377, 2628, 3370
MabI ACCWGGT 1 cut(s) 838
MaeIII GTNAC 7 cut(s) 10, 140, 1493, 1748, 2592, 2855, 3226
MbiI CCGCTC 1 cut(s) 1818
MfeI CAATTG 3 cut(s) 166, 1811, 3186
MflI RGATCY 3 cut(s) 2474, 2544, 3303
MhlI GDGCHC 5 cut(s) 220, 272, 699, 1269, 2679
MlsI TGGCCA 2 cut(s) 1290, 3182
MluNI TGGCCA 2 cut(s) 1290, 3182
Mly113I GGCGCC 1 cut(s) 761
MlyI GAGTC 8 cut(s) 240, 355, 547, 664, 838, 2385, 2457, 3157
MmeI TCCRAC 3 cut(s) 560, 857, 2973
Mox20I TGGCCA 2 cut(s) 1290, 3182
MroXI GAANNNNTTC 1 cut(s) 2662
MscI TGGCCA 2 cut(s) 1290, 3182
MslI CAYNNNNRTG 3 cut(s) 1431, 2327, 3042
Msp20I TGGCCA 2 cut(s) 1290, 3182
MspA1I CMGCKG 1 cut(s) 2618
MspI CCGG 7 cut(s) 103, 373, 561, 618, 716, 826, 2540
MspR9I CCNGG 6 cut(s) 338, 717, 840, 1271, 2541, 3179
MunI CAATTG 3 cut(s) 166, 1811, 3186
Mva1269I GAATGC 1 cut(s) 2614
MvaI CCWGG 4 cut(s) 338, 840, 1271, 3179
MvnI CGCG 1 cut(s) 266
NarI GGCGCC 1 cut(s) 761
NciI CCSGG 2 cut(s) 717, 2541
NdeI CATATG 1 cut(s) 1228
NheI GCTAGC 1 cut(s) 53
NlaIV GGNNCC 9 cut(s) 271, 343, 436, 762, 877, 2023, 2476, 2939, 3397
NmeAIII GCCGAG 1 cut(s) 3102
NmuCI GTSAC 3 cut(s) 140, 2855, 3226
NspI RCATGY 4 cut(s) 955, 2929, 3116, 3502
NspV TTCGAA 1 cut(s) 1653
OliI CACNNNNGTG 2 cut(s) 1431, 2327
PaeI GCATGC 1 cut(s) 3502
PagI TCATGA 1 cut(s) 3409
PceI AGGCCT 2 cut(s) 1383, 1797
PciI ACATGT 2 cut(s) 951, 3112
PciSI GCTCTTC 2 cut(s) 117, 525
PcsI WCGNNNNNNNCGW 3 cut(s) 226, 709, 734
PctI GAATGC 1 cut(s) 2614
PdmI GAANNNNTTC 1 cut(s) 2662
PflMI CCANNNNNTGG 4 cut(s) 839, 1092, 2580, 3209
PfoI TCCNGGA 1 cut(s) 1269
PinAI ACCGGT 1 cut(s) 102
PleI GAGTC 8 cut(s) 239, 355, 546, 664, 837, 2385, 2457, 3157
PluTI GGCGCC 1 cut(s) 764
PpsI GAGTC 8 cut(s) 239, 355, 546, 664, 837, 2385, 2457, 3157
PpuMI RGGWCCY 1 cut(s) 435
PscI ACATGT 2 cut(s) 951, 3112
Psp124BI GAGCTC 1 cut(s) 2679
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 4 cut(s) 336, 838, 1269, 3177
PspGI CCWGG 4 cut(s) 336, 838, 1269, 3177
PspN4I GGNNCC 9 cut(s) 271, 343, 436, 762, 877, 2023, 2476, 2939, 3397
PspPI GGNCC 7 cut(s) 435, 1073, 1820, 2022, 2111, 2255, 2417
PspPPI RGGWCCY 1 cut(s) 435
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstI CTGCAG 2 cut(s) 2623, 3565
PstNI CAGNNNCTG 1 cut(s) 2126
PsuI RGATCY 3 cut(s) 2474, 2544, 3303
PvuII CAGCTG 1 cut(s) 2618
RsaI GTAC 8 cut(s) 343, 1219, 1940, 1971, 2190, 3029, 3051, 3556
RsaNI GTAC 8 cut(s) 342, 1218, 1939, 1970, 2189, 3028, 3050, 3555
RseI CAYNNNNRTG 3 cut(s) 1431, 2327, 3042
SacI GAGCTC 1 cut(s) 2679
SapI GCTCTTC 2 cut(s) 117, 525
Sau96I GGNCC 7 cut(s) 435, 1073, 1820, 2022, 2111, 2255, 2417
ScaI AGTACT 1 cut(s) 3556
SchI GAGTC 8 cut(s) 240, 355, 547, 664, 838, 2385, 2457, 3157
ScrFI CCNGG 6 cut(s) 338, 717, 840, 1271, 2541, 3179
SduI GDGCHC 5 cut(s) 220, 272, 699, 1269, 2679
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 6 cut(s) 788, 1715, 2006, 2377, 2628, 3370
SfcI CTRYAG 4 cut(s) 611, 2619, 3510, 3561
SfoI GGCGCC 1 cut(s) 762
SfuI TTCGAA 1 cut(s) 1653
SinI GGWCC 5 cut(s) 435, 1073, 2022, 2111, 2417
SmiMI CAYNNNNRTG 3 cut(s) 1431, 2327, 3042
SmlI CTYRAG 4 cut(s) 1741, 2363, 2643, 2942
SmoI CTYRAG 4 cut(s) 1741, 2363, 2643, 2942
SpeI ACTAGT 1 cut(s) 2510
SphI GCATGC 1 cut(s) 3502
SseBI AGGCCT 2 cut(s) 1383, 1797
SspDI GGCGCC 1 cut(s) 760
SspI AATATT 2 cut(s) 528, 1606
SstI GAGCTC 1 cut(s) 2679
StuI AGGCCT 2 cut(s) 1383, 1797
StyD4I CCNGG 6 cut(s) 336, 715, 838, 1269, 2539, 3177
StyI CCWWGG 1 cut(s) 2395
TaaI ACNGT 4 cut(s) 332, 423, 689, 701
TaqI TCGA 7 cut(s) 229, 606, 665, 703, 728, 1653, 2311
TatI WGTACW 2 cut(s) 1938, 3554
TauI GCSGC 7 cut(s) 53, 573, 601, 660, 761, 2415, 2570
TscAI CASTG 8 cut(s) 145, 694, 1147, 1438, 1765, 2329, 3316, 3486
TseFI GTSAC 3 cut(s) 140, 2855, 3226
Tsp45I GTSAC 3 cut(s) 140, 2855, 3226
TspDTI ATGAA 6 cut(s) 373, 448, 1441, 2655, 2718, 3060
TspGWI ACGGA 1 cut(s) 3230
TspRI CASTG 8 cut(s) 145, 694, 1147, 1438, 1765, 2329, 3316, 3486
Van91I CCANNNNNTGG 4 cut(s) 839, 1092, 2580, 3209
VneI GTGCAC 1 cut(s) 216
VpaK11BI GGWCC 5 cut(s) 435, 1073, 2022, 2111, 2417
XagI CCTNNNNNAGG 2 cut(s) 285, 2118
XbaI TCTAGA 3 cut(s) 1480, 2248, 2344
XceI RCATGY 4 cut(s) 955, 2929, 3116, 3502
XcmI CCANNNNNNNNNTGG 3 cut(s) 1089, 1254, 2055
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 2662
ZrmI AGTACT 1 cut(s) 3556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.