MD07G1035900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000058
Physical Location & Seq
Reverse (-)
2924642 .. 2930860
6219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1035900.v1.1.491

Sequence Viewer

Length: 3489 bp
ATGGCGAGAACTCGTTTCCTCTCGTCGACAACTTTGTCGTTGCTGCTATTCCAATACTGTTGTGTATACATACCTATCTTAATAACGGGTTCCGTAGCAGCACAAACCAACATCAGCACAGATCAGTCTGCTCTTCTTGCCCTCAAATCCCATATCACCATTGATCCTCAAAACATCTTGACCACCAACTGGTCTACCTCCATTTCCGACATTTGCAACTGGGTTGGCGTTACTTGCAGTGCACGCCACCTTAGAGTGACAACCTTGAATCTCTCCTACATGGGTCTCACTGGAACCATCCCTCCCCACCTAGGCAACCTCTCATTTCTTGTTCAATTGGAATTCAGAAACAACAGTTTTGGTGGTACCTTGCCCCCGGAATTGTCTAATCTACGCAGGCTGAAGTTGATTAGCTTTGAATTCAATGACTTTGAAGGAACCATTCCATCATGGTTCGGGTTGTTATCTAAACTTCAAACCTTCAATTTGTACGGTAATCAATTTTCAGGTCCCATACCAAACGCCATCTTCAACTTATCTGCACTTCAAGTACTTAATCTAAGAAGCAACCAGCTATCCGGTACCATACCAAGAGAAATCGAGAGGTTAACAGTGTTGCAAGAGATATTACTTGGAAATAACAATTTCAAAGGTAGCATAGCAAGAGAAATTGGGAACTTAACCATGCTCAGGAATATATACCTTGATTTTAACATGCTCGAAGAAATTCCAAACTTGATTGGCAGTAAAGATGAGGTGGAGAAGTTGTATGTGCAGGCTAATGCCCTAAAAGGCCCCATTCCTTTGGCTATCTTCAACATGTCCTCTTTGACAATTTTGGCTCTAAACGGAAACAACTTAAGTGGTAGTTTTCCGGACAATATATGCCAACATCTTCCAAGCATCCAGGTGTTAAATTCGGCTCACAACCAGTTTGATGGTCCACTTCCACACAAGTTATGGCAGTGCAAAGAGCTCCTTAGTTTGGTATTGGACTATAATAATTTCAGTGGAAGCATACCAAGAAGTATTGGAAACTTGACCCAGTTAACACAGCTTGGTTTTGGCAACAACACCTTAATAGGTATTATACCATATGAAGTTGGCAATCTTCAAAGTCTACAGTATTTGTCACTGGATTTTAACAATCTCAATGGCCCCTTTCCATCCACAATCTTCAATATGTCAAAGATGACAGTACTTTCACTTGTTGGTAATCAACTCTCAGGTAGCCTCCCAGCAAACATAGGCCTTGGAATTCCGAACCTACAAAGTATATATTTAGGAATGAATGAACTCAGCGGAGTCATTCCCAATTTTATCTCCAATGGCTCTAAGCTCAATCATGTAGACATTTCCGACAATTCATTTTCCGGGTTTATTCCTAACAAACTATGTGCCTTAGCAAACCTTGAGTATCTTAGCTTATACCAAAATAATTTGACGGTTGACACTTCAACTCCAGAAGTAAATTTCCTTTCTTGTTTAGGTAATCTTAGAAATCTTGGGGTACTCATCCTGGGACGTAATCCTTTAAACGCTCCTCTTCCTATTTCTTTTCAGAATTACTCTACATCGCTTCAAAAAATTTCTTTAAGCGAATGCAAATTGAAGGGTAGCATTCCCAATGATATTGGCAACTTGAGCAGCTTGATATCCTTAAACCTGGTATCCAATCAATTGAGCGGGTTAATTCCAACTTCGATAGGAAGACTAGGGAGTCTCCAAGGTTTGTATTTGGTTGATAACAAGTTGCAAGGATATATTCCCGATAAACTTTGTCAACTAAACAAACTTTCTGTATTATATTTGAGTCAGAATCAACTTTCTGGTTTTATACCTTCTTGCTTAGGCAATCTATCTGCAAATCTTGGAATTCTATCATTAGCCTCCAATTTGTTAACTTCTACACTGCCATCTACATTGTGGGAACTAGAACAAGTCTGGTTCTTCAACTTGTCATCAAATCTTCTAAATGGATCTCTGTCACAAGATATCGGCCAGTTGAAAGTTTTGTCATACATGGATCTATCAAGCAACAATTTATTTGGTGTTATACCAAGCAGCGTTGGGGATCTTCTACATTTAATTAGTCTCTCCTTGGCAGATAATAATTTTGAAGGCCCTATTCCCAGTTCATTCGGCAAATTGACAGGCCTGATACAGTTGGATTTATCCAAGAACAGTTTGTCTGAAGCAATTCCAAAGTCGCTAGAAGCATTCTTGAATCTGAAGTACCTGAATTTTTCTTTCAACCAACTCCAAGGGGAAATTCCAGAAGGCGGACCTTTCAAAAACTTCTCTGCTCCATCATTTGTCTCGAATAAGGCGTTGTGTGGTGCAGCTCGATTCCATGTCCCACCATGCAAAAACAATACACTTCAACCAAATTCAAGGAAAGCTGGTTCATCCAACTTGAAATATATTATTCCAGCAATAATCATAGCAACAATATTCCTAGTAGCCTTTGTGTCTATACTGCAAAGGAAAAGGAAAATTGAAGATGCAACAGAGGCTACCGTGTTGCCTCAATCTCTTTGGAGAAGAGTTTCGCACCTGGAACTTCTAAGGGCGACAAATGGATTTAACGATAGCAACTTACTTGGAAGTGGGGGTTTTGGTTCAGTATATAAAGGGACACTCTCAGATGGGATAGATGTTGCAGTAAAGATTTTCGATTTGCAGCTAGAAGGGGCTTTCAAGAGTTTTGAGAGGGAATGTGAAACGCTAAGTAATATTCGGCACCGAAATCTTATCAAAATCATTAGCTGTTGCAGTCAATTGGATTTCAAAGCCTTGGTACTGAACTATATGCCTAATGGTAGCCTTGAGAAGTGGTTGTATTCTCAAAACTTTTCTTTTAATATCCTAAAGAGGTTGAATATAATGGTAGATGTTGCATTGGCATTGGAATATCTACACCATGGTTATTCAGTGCCAATTGTCCATTGTGATTTGAAGCCAAGCAATATCCTACTAGATGATGATATGGTTGCACATGTTGCGGATTTTGGCATTGCAAAACTCTTGGGTTTCGGGGATTCCATGACCCGAACCATGACCCTAGCCACAGTTGGGTATATGGCTCCAGAGTACGGGATGGATGGAATTGTTTCTACGCAAGGGGATGTGTACAGTTTTGGTATTGTAGTCATGGAAACATTCACAAAAATGAAGCCTACGAATGAAATGTTTGTTGAGGAAATGAATCTAAGGCAATGGGTTGCAAATTCACTTTCATCAGATGCAATAGCTGAAGTTGTGGATGCCAATTTACTTGTAACACAAGAGGAAGATCCTGGTTTTGTGACTAAGATAGATTGCTTATCTTCCATTATGCGATTAGCTCTTGCTTGCTGTGCAGAGCTACCAGGCGAGAGAATAAACATGCAAGAAGCTGTAGCCACACTCAAAAAAATAAAAATCAAGTTTTTGCGGGATGCTGCAAGAAGTGTGCAGTTAAACCGTCCTCTTGTTCATGCACTTTGA

Protein Analysis

1163

Amino Acids

127.44

Weight (kDa)

6.28

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 39 - 80 2.5e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 133 - 192 5e-06 Leucine rich repeat
LRR_14 PF23598 148 - 240 2.7e-08 Leucine-rich repeat region
LRR_14 PF23598 343 - 505 1.3e-07 Leucine-rich repeat region
LRR_8 PF13855 421 - 481 2.8e-07 Leucine rich repeat
LRR_14 PF23598 531 - 630 3e-06 Leucine-rich repeat region
LRR_8 PF13855 550 - 609 5.5e-08 Leucine rich repeat
LRR_14 PF23598 641 - 754 1.1e-08 Leucine-rich repeat region
Pkinase PF00069 863 - 1135 2.3e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 866 - 1135 3.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 34, 1719
Acc65I GGTACC 2 cut(s) 365, 581
AccB1I GGYRCC 3 cut(s) 365, 581, 2742
AccB7I CCANNNNNTGG 1 cut(s) 189
AccBSI CCGCTC 1 cut(s) 1686
AccI GTMKAC 5 cut(s) 26, 66, 194, 1120, 1350
AccIII TCCGGA 1 cut(s) 874
AciI CCGC 5 cut(s) 1302, 1686, 2283, 3005, 3436
AclWI GGATC 5 cut(s) 158, 1987, 2034, 2082, 3290
AcoI YGGCCR 1 cut(s) 1999
AcuI CTGAAG 4 cut(s) 422, 2214, 2252, 3276
AfiI CCNNNNNNNGG 7 cut(s) 189, 311, 690, 985, 2282, 3075, 3095
AflII CTTAAG 1 cut(s) 859
AflIII ACRYGT 2 cut(s) 819, 2998
AjnI CCWGG 6 cut(s) 906, 1518, 1665, 2556, 3298, 3370
AjuI GAANNNNNNNTTGG 2 cut(s) 2256, 2288
AloI GAACNNNNNNTCC 4 cut(s) 286, 318, 2389, 2421
Alw21I GWGCWC 2 cut(s) 244, 978
Alw26I GTCTC 4 cut(s) 290, 1727, 2099, 2323
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 5 cut(s) 158, 1987, 2034, 2082, 3290
Aor13HI TCCGGA 1 cut(s) 874
AoxI GGCC 6 cut(s) 793, 1156, 1249, 1999, 2122, 2155
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 7 cut(s) 43, 98, 1647, 2064, 2342, 2683, 3443
Asp700I GAANNNNTTC 4 cut(s) 726, 2199, 2548, 3112
Asp718I GGTACC 2 cut(s) 365, 581
AspA2I CCTAGG 1 cut(s) 310
AspS9I GGNCC 6 cut(s) 509, 794, 941, 1157, 2123, 2285
AsuC2I CCSGG 2 cut(s) 377, 1375
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 3 cut(s) 509, 941, 2285
AvrII CCTAGG 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 3 cut(s) 365, 581, 2742
BanII GRGCYC 1 cut(s) 978
BbsI GAAGAC 1 cut(s) 1717
Bbv12I GWGCWC 2 cut(s) 244, 978
BbvI GCAGC 7 cut(s) 30, 110, 1659, 2076, 2354, 2695, 3430
BciT130I CCWGG 6 cut(s) 908, 1520, 1667, 2558, 3300, 3372
BciVI GTATCC 1 cut(s) 1681
BcnI CCSGG 2 cut(s) 377, 1375
BcoDI GTCTC 4 cut(s) 290, 1727, 2099, 2323
BfaI CTAG 8 cut(s) 311, 1715, 1934, 2213, 2459, 2687, 2978, 3065
BfmI CTRYAG 2 cut(s) 1121, 3399
BfrI CTTAAG 1 cut(s) 859
BfuI GTATCC 1 cut(s) 1681
BisI GCNGC 7 cut(s) 44, 99, 1648, 2065, 2343, 2684, 3444
BlnI CCTAGG 1 cut(s) 310
BlsI GCNGC 7 cut(s) 45, 100, 1649, 2066, 2344, 2685, 3445
BmcAI AGTACT 2 cut(s) 552, 1200
Bme1390I CCNGG 8 cut(s) 377, 908, 1375, 1520, 1667, 2558, 3300, 3372
Bme18I GGWCC 3 cut(s) 509, 941, 2285
BmgT120I GGNCC 6 cut(s) 509, 794, 941, 1157, 2123, 2285
BmrFI CCNGG 8 cut(s) 377, 908, 1375, 1520, 1667, 2558, 3300, 3372
BmrI ACTGGG 3 cut(s) 229, 1039, 2127
BmsI GCATC 5 cut(s) 912, 2494, 3235, 3256, 3430
BmuI ACTGGG 3 cut(s) 229, 1039, 2127
BpiI GAAGAC 1 cut(s) 1717
BpmI CTGGAG 2 cut(s) 1446, 3072
Bpu10I CCTNAGC 3 cut(s) 689, 1402, 1849
BpuEI CTTGAG 3 cut(s) 1433, 1663, 2849
BpuMI CCSGG 2 cut(s) 377, 1375
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 9 cut(s) 310, 375, 1252, 1519, 1726, 2100, 2263, 2796, 2923
BsaWI WCCGGW 2 cut(s) 578, 874
BsaXI ACNNNNNCTCC 6 cut(s) 286, 316, 752, 782, 1444, 1474
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 311, 690, 985, 2282, 3075, 3095
Bse1I ACTGG 8 cut(s) 194, 224, 295, 931, 1045, 1140, 2002, 2133
Bse3DI GCAATG 2 cut(s) 3015, 3224
BseAI TCCGGA 1 cut(s) 874
BseBI CCWGG 6 cut(s) 908, 1520, 1667, 2558, 3300, 3372
BseDI CCNNGG 9 cut(s) 310, 375, 1252, 1519, 1726, 2100, 2263, 2796, 2923
BseLI CCNNNNNNNGG 7 cut(s) 189, 311, 690, 985, 2282, 3075, 3095
BseMI GCAATG 2 cut(s) 3015, 3224
BseMII CTCAG 4 cut(s) 703, 1239, 1312, 2658
BseNI ACTGG 8 cut(s) 194, 224, 295, 931, 1045, 1140, 2002, 2133
BseRI GAGGAG 1 cut(s) 1533
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 7 cut(s) 30, 110, 1659, 2076, 2354, 2695, 3430
BseYI CCCAGC 1 cut(s) 1237
BsgI GTGCAG 5 cut(s) 525, 794, 2361, 3381, 3476
BshFI GGCC 6 cut(s) 795, 1158, 1251, 2001, 2124, 2157
BshNI GGYRCC 3 cut(s) 365, 581, 2742
BsiHKAI GWGCWC 2 cut(s) 244, 978
BsiSI CCGG 4 cut(s) 377, 579, 875, 1374
BslFI GGGAC 4 cut(s) 495, 1536, 2342, 2650
BslI CCNNNNNNNGG 7 cut(s) 189, 311, 690, 985, 2282, 3075, 3095
BsmAI GTCTC 4 cut(s) 290, 1727, 2099, 2323
BsmFI GGGAC 4 cut(s) 495, 1536, 2342, 2650
BsmI GAATGC 3 cut(s) 1607, 1620, 2219
BsnI GGCC 6 cut(s) 795, 1158, 1251, 2001, 2124, 2157
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 244, 978
Bsp13I TCCGGA 1 cut(s) 874
Bsp1407I TGTACA 1 cut(s) 3132
Bsp143I GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
Bsp19I CCATGG 1 cut(s) 2923
BspACI CCGC 5 cut(s) 1302, 1686, 2283, 3005, 3436
BspANI GGCC 6 cut(s) 795, 1158, 1251, 2001, 2124, 2157
BspCNI CTCAG 4 cut(s) 702, 1238, 1311, 2657
BspEI TCCGGA 1 cut(s) 874
BspPI GGATC 5 cut(s) 158, 1987, 2034, 2082, 3290
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 3 cut(s) 365, 581, 2742
BspTI CTTAAG 1 cut(s) 859
BspTNI GGTCTC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 1686
BsrDI GCAATG 2 cut(s) 3015, 3224
BsrGI TGTACA 1 cut(s) 3132
BsrI ACTGG 8 cut(s) 194, 224, 295, 931, 1045, 1140, 2002, 2133
BssECI CCNNGG 9 cut(s) 310, 375, 1252, 1519, 1726, 2100, 2263, 2796, 2923
BssMI GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 7 cut(s) 310, 1252, 1726, 2100, 2263, 2796, 2923
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 6 cut(s) 908, 1520, 1667, 2558, 3300, 3372
Bst6I CTCTTC 3 cut(s) 138, 1551, 2539
BstAFI CTTAAG 1 cut(s) 859
BstAPI GCANNNNNTGC 1 cut(s) 3002
BstAUI TGTACA 1 cut(s) 3132
BstC8I GCNNGC 4 cut(s) 244, 398, 777, 3355
BstDSI CCRYGG 1 cut(s) 2923
BstKTI GATC 6 cut(s) 124, 166, 1982, 2029, 2077, 3298
BstMAI GTCTC 4 cut(s) 290, 1727, 2099, 2323
BstMBI GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
BstMWI GCNNNNNNNGC 7 cut(s) 137, 234, 243, 1644, 2513, 3002, 3359
BstNI CCWGG 6 cut(s) 908, 1520, 1667, 2558, 3300, 3372
BstNSI RCATGY 4 cut(s) 718, 823, 3002, 3391
BstSCI CCNGG 8 cut(s) 375, 906, 1373, 1518, 1665, 2556, 3298, 3370
BstSFI CTRYAG 2 cut(s) 1121, 3399
BstSLI GKGCMC 1 cut(s) 244
BstV1I GCAGC 7 cut(s) 30, 110, 1659, 2076, 2354, 2695, 3430
BstV2I GAAGAC 1 cut(s) 1717
BstX2I RGATCY 4 cut(s) 1979, 2026, 2074, 3295
BstXI CCANNNNNNTGG 2 cut(s) 805, 938
BstYI RGATCY 4 cut(s) 1979, 2026, 2074, 3295
BstZ17I GTATAC 1 cut(s) 67
BsuI GTATCC 1 cut(s) 1681
BsuRI GGCC 6 cut(s) 795, 1158, 1251, 2001, 2124, 2157
BtgI CCRYGG 1 cut(s) 2923
BtgZI GCGATG 1 cut(s) 1560
BtsI GCAGTG 3 cut(s) 244, 971, 1910
BtsIMutI CAGTG 8 cut(s) 244, 288, 618, 971, 1015, 1133, 1910, 2940
Cac8I GCNNGC 4 cut(s) 244, 398, 777, 3355
Cfr13I GGNCC 6 cut(s) 509, 794, 941, 1157, 2123, 2285
CsiI ACCWGGT 1 cut(s) 1665
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
DpnI GATC 6 cut(s) 123, 165, 1981, 2028, 2076, 3297
DpnII GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
DraI TTTAAA 1 cut(s) 1536
DrdI GACNNNNNNGTC 2 cut(s) 34, 1719
DseDI GACNNNNNNGTC 2 cut(s) 34, 1719
EaeI YGGCCR 1 cut(s) 1999
Eam1104I CTCTTC 3 cut(s) 138, 1551, 2539
EarI CTCTTC 3 cut(s) 138, 1551, 2539
EciI GGCGGA 1 cut(s) 2298
Ecl136II GAGCTC 1 cut(s) 976
Eco130I CCWWGG 7 cut(s) 310, 1252, 1726, 2100, 2263, 2796, 2923
Eco147I AGGCCT 2 cut(s) 1251, 2157
Eco24I GRGCYC 1 cut(s) 978
Eco31I GGTCTC 1 cut(s) 290
Eco32I GATATC 2 cut(s) 1656, 1996
Eco47I GGWCC 3 cut(s) 509, 941, 2285
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 4 cut(s) 422, 2214, 2252, 3276
EcoICRI GAGCTC 1 cut(s) 976
EcoO109I RGGNCCY 3 cut(s) 509, 794, 2123
EcoRI GAATTC 4 cut(s) 341, 419, 1257, 1875
EcoRII CCWGG 6 cut(s) 906, 1518, 1665, 2556, 3298, 3370
EcoRV GATATC 2 cut(s) 1656, 1996
EcoT14I CCWWGG 7 cut(s) 310, 1252, 1726, 2100, 2263, 2796, 2923
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 7 cut(s) 310, 1252, 1726, 2100, 2263, 2796, 2923
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FaqI GGGAC 4 cut(s) 495, 1536, 2342, 2650
FauI CCCGC 2 cut(s) 1679, 3429
FauNDI CATATG 1 cut(s) 1096
FblI GTMKAC 5 cut(s) 26, 66, 194, 1120, 1350
Fnu4HI GCNGC 7 cut(s) 44, 99, 1648, 2065, 2343, 2684, 3444
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 7 cut(s) 44, 99, 1648, 2065, 2343, 2684, 3444
FspBI CTAG 8 cut(s) 311, 1715, 1934, 2213, 2459, 2687, 2978, 3065
GluI GCNGC 7 cut(s) 44, 99, 1648, 2065, 2343, 2684, 3444
GsaI CCCAGC 1 cut(s) 1241
GsuI CTGGAG 2 cut(s) 1446, 3072
HaeIII GGCC 6 cut(s) 795, 1158, 1251, 2001, 2124, 2157
HapII CCGG 4 cut(s) 377, 579, 875, 1374
HincII GTYRAC 6 cut(s) 27, 609, 1050, 1450, 1784, 1902
HindII GTYRAC 6 cut(s) 27, 609, 1050, 1450, 1784, 1902
HinfI GANTC 9 cut(s) 268, 1305, 1720, 1813, 1819, 2227, 2349, 3041, 3208
HpaI GTTAAC 3 cut(s) 609, 1050, 1902
HpaII CCGG 4 cut(s) 377, 579, 875, 1374
HphI GGTGA 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 7 cut(s) 428, 490, 1606, 1851, 2114, 2273, 2684
HpyCH4IV ACGT 1 cut(s) 1525
HpyF10VI GCNNNNNNNGC 7 cut(s) 137, 234, 243, 1644, 2513, 3002, 3359
HpySE526I ACGT 1 cut(s) 1525
Kpn2I TCCGGA 1 cut(s) 874
KpnI GGTACC 2 cut(s) 369, 585
KspAI GTTAAC 3 cut(s) 609, 1050, 1902
Kzo9I GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 4 cut(s) 981, 1546, 2311, 3091
Lsp1109I GCAGC 7 cut(s) 30, 110, 1659, 2076, 2354, 2695, 3430
LweI GCATC 5 cut(s) 912, 2494, 3235, 3256, 3430
MabI ACCWGGT 1 cut(s) 1665
MaeI CTAG 8 cut(s) 311, 1715, 1934, 2213, 2459, 2687, 2978, 3065
MaeII ACGT 1 cut(s) 1525
MaeIII GTNAC 6 cut(s) 229, 256, 1131, 1986, 3280, 3307
MalI GATC 6 cut(s) 123, 165, 1981, 2028, 2076, 3297
MbiI CCGCTC 1 cut(s) 1686
MboI GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
MfeI CAATTG 4 cut(s) 335, 1679, 2780, 2940
MflI RGATCY 4 cut(s) 1979, 2026, 2074, 3295
MhlI GDGCHC 2 cut(s) 244, 978
MlyI GAGTC 3 cut(s) 1314, 1729, 1822
MmeI TCCRAC 5 cut(s) 231, 1383, 1721, 2148, 2436
MroI TCCGGA 1 cut(s) 874
MroXI GAANNNNTTC 4 cut(s) 726, 2199, 2548, 3112
MslI CAYNNNNRTG 2 cut(s) 908, 3170
MspA1I CMGCKG 1 cut(s) 1302
MspCI CTTAAG 1 cut(s) 859
MspI CCGG 4 cut(s) 377, 579, 875, 1374
MspR9I CCNGG 8 cut(s) 377, 908, 1375, 1520, 1667, 2558, 3300, 3372
MunI CAATTG 4 cut(s) 335, 1679, 2780, 2940
Mva1269I GAATGC 3 cut(s) 1607, 1620, 2219
MvaI CCWGG 6 cut(s) 908, 1520, 1667, 2558, 3300, 3372
MwoI GCNNNNNNNGC 7 cut(s) 137, 234, 243, 1644, 2513, 3002, 3359
NciI CCSGG 2 cut(s) 377, 1375
NcoI CCATGG 1 cut(s) 2923
NdeI CATATG 1 cut(s) 1096
NdeII GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
NmuCI GTSAC 4 cut(s) 256, 1131, 1986, 3307
NspI RCATGY 4 cut(s) 718, 823, 3002, 3391
PceI AGGCCT 2 cut(s) 1251, 2157
PciI ACATGT 2 cut(s) 819, 2998
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 2327
PctI GAATGC 3 cut(s) 1607, 1620, 2219
PdmI GAANNNNTTC 4 cut(s) 726, 2199, 2548, 3112
PfeI GAWTC 6 cut(s) 268, 1819, 2227, 2349, 3041, 3208
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 7 cut(s) 45, 100, 1649, 2066, 2344, 2685, 3445
PleI GAGTC 3 cut(s) 1313, 1728, 1821
PpsI GAGTC 3 cut(s) 1313, 1728, 1821
PpuMI RGGWCCY 1 cut(s) 509
PscI ACATGT 2 cut(s) 819, 2998
Psp124BI GAGCTC 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 6 cut(s) 906, 1518, 1665, 2556, 3298, 3370
PspFI CCCAGC 1 cut(s) 1237
PspGI CCWGG 6 cut(s) 906, 1518, 1665, 2556, 3298, 3370
PspPI GGNCC 6 cut(s) 509, 794, 941, 1157, 2123, 2285
PspPPI RGGWCCY 1 cut(s) 509
PsuI RGATCY 4 cut(s) 1979, 2026, 2074, 3295
RseI CAYNNNNRTG 2 cut(s) 908, 3170
SacI GAGCTC 1 cut(s) 978
SalI GTCGAC 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 138
SatI GCNGC 7 cut(s) 44, 99, 1648, 2065, 2343, 2684, 3444
Sau3AI GATC 6 cut(s) 121, 163, 1979, 2026, 2074, 3295
Sau96I GGNCC 6 cut(s) 509, 794, 941, 1157, 2123, 2285
ScaI AGTACT 2 cut(s) 552, 1200
SchI GAGTC 3 cut(s) 1314, 1729, 1822
ScrFI CCNGG 8 cut(s) 377, 908, 1375, 1520, 1667, 2558, 3300, 3372
SduI GDGCHC 2 cut(s) 244, 978
SexAI ACCWGGT 1 cut(s) 1665
SfaNI GCATC 5 cut(s) 912, 2494, 3235, 3256, 3430
SfcI CTRYAG 2 cut(s) 1121, 3399
SinI GGWCC 3 cut(s) 509, 941, 2285
SmiMI CAYNNNNRTG 2 cut(s) 908, 3170
SmlI CTYRAG 4 cut(s) 859, 1412, 1642, 2828
SmoI CTYRAG 4 cut(s) 859, 1412, 1642, 2828
SseBI AGGCCT 2 cut(s) 1251, 2157
SsiI CCGC 5 cut(s) 1302, 1686, 2283, 3005, 3436
SspI AATATT 2 cut(s) 2454, 2737
SspMI CTAG 8 cut(s) 311, 1715, 1934, 2213, 2459, 2687, 2978, 3065
SstI GAGCTC 1 cut(s) 978
StuI AGGCCT 2 cut(s) 1251, 2157
StyD4I CCNGG 8 cut(s) 375, 906, 1373, 1518, 1665, 2556, 3298, 3370
StyI CCWWGG 7 cut(s) 310, 1252, 1726, 2100, 2263, 2796, 2923
TaiI ACGT 1 cut(s) 1528
TaqI TCGA 7 cut(s) 26, 600, 720, 1703, 2321, 2347, 2676
TatI WGTACW 3 cut(s) 550, 1198, 3132
TfiI GAWTC 6 cut(s) 268, 1819, 2227, 2349, 3041, 3208
TscAI CASTG 8 cut(s) 244, 295, 618, 971, 1015, 1140, 1917, 2940
TseFI GTSAC 4 cut(s) 256, 1131, 1986, 3307
TseI GCWGC 7 cut(s) 43, 98, 1647, 2064, 2342, 2683, 3443
Tsp45I GTSAC 4 cut(s) 256, 1131, 1986, 3307
TspGWI ACGGA 2 cut(s) 82, 864
TspRI CASTG 8 cut(s) 244, 295, 618, 971, 1015, 1140, 1917, 2940
Van91I CCANNNNNTGG 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 859
VneI GTGCAC 1 cut(s) 240
VpaK11BI GGWCC 3 cut(s) 509, 941, 2285
XceI RCATGY 4 cut(s) 718, 823, 3002, 3391
XcmI CCANNNNNNNNNTGG 2 cut(s) 957, 1923
XmaJI CCTAGG 1 cut(s) 310
XmiI GTMKAC 5 cut(s) 26, 66, 194, 1120, 1350
XmnI GAANNNNTTC 4 cut(s) 726, 2199, 2548, 3112
XspI CTAG 8 cut(s) 311, 1715, 1934, 2213, 2459, 2687, 2978, 3065
ZrmI AGTACT 2 cut(s) 552, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.