MD05G1297700.v1.1

Leucine rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000058
Physical Location & Seq
Forward (+)
43142138 .. 43146937
4800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1297700.v1.1.491

Sequence Viewer

Length: 3561 bp
ATGAAAAATATATACAAACTTCAAGTTGCAGACACGTATGTCTTCTTGGTTTTCAGAAAGTGTTTGCTGTATAGGACAATGGCGAGAACTCGTTCCCTCTTGTCGATAGCGTTGTTGCTCGTCCTGAGCTGTTGTATGTATCTGCGGAACTCATTAATAGGCACAGTAGCAGCACAAACCAACATCAGCACAGACCAAGCTGCTCTTCTTGCTTTCAAAGCCCATATAACTAGTGACCCCCGAAACATATTGACCACCAACTGGTCTACCACGAATTCCAATATCTGCAACTGGGTTGGCATTACTTGCGGTGTAAGCCACCTTAGAGTCACAACCGTGAATCTGTCTTACATGGGTCTCAGAGGCTCTATTCCTCCTCACCTAGGCAACCTCTCATTTCTTGTTCAGCTGGAATTCAGAAACAACAGTTTCCATGGTACCTTGCCACCAGAATTGTCTCGTCTACGGAGGTTGAAGTTGATTAGGTTCAGCTTTAACGACTTTATAGGAACAATTCCATCATGGTTCGGGCTCTTATCTGAACTTCAAACCTTCGATTTGTATGGTAATCAATTTTCAGGTTTCATACCGAATGCCATCTTCAACTTATCTGCACTGCAAGTACTTGATCTAAGAAACAACCAGCTATCTGGTAGCATACCAAGAGATGTCGGGAAGTTAAAAATGTTGAAGGAGATATCACTTGGAGACAACGATTTCACAGGTAGAATACCAAGAGAAATCGGGAACTTAACCATGCTGAAGAATATATACCTTGACTTTAACATGTTCGAAGAAATTCCAAACTTGATTGGTAGTAAAGATCAGGTGGAGAAGTTGTACGTGCAGGCTAATGCCCTAAAAGGACCTGTTCCTTTGGCTGTCTTCAACATGTCTTCTTTGAAAATTTTGGCTCTAACAGCAAACTACTTGACTGGTAGCATTCCGGACGATTTATGTCAGCATCTTCCAAGTATCCAGATCTTGAGTTTGGCTCGCAACAAACTTGATGGTCCACTTCCATCCAAATTATGGCAATGCAAAGAGCTTCTCCAGTTCTCTTTAGACTATAACAACTTCAGTGGAAGCATACCAAAAAGCATCGGGAACTCTACCCAGTTAGAAAGGATTGGCATTAGCTACAACAACTTGACAGGTACCATACCAGATGAGATTGGTCATCTTAAAAATTTACACAGATTGTCACTGCATGTTAATAATCTCCATGGCCCCATCCCATCCACAATATTCAATATGTCCAAGATCACAACTATTTCTCTTCCTGAAAATCAACTCTCAGGCAACCTTCCTGCATACATAGGTCTTGGGGTCCCAGACCTACAAATGTTTTATGTAGGTGGCAATCAACTCACTGGAGCATTTCCCAACTTTATGTCCAATGGTTCTAAGCTCACAAGTCTAGATATGTCCAAGAACTCATTTTATGGGTTTATTCCAACCAAGCTCTGTCTCTTGAAAACCCTTGAGTATCTTAGCTTAGGCACAAATAATTTGGCGATTGATACTTCAACTCCAGAAGTAAACATCCCTTCTTGTTTTGCTAATCTCACAAATCTTCGGGGATTCCATTTATCAAATAATCCATTAAACACCACTCTTCCGGTTTCCTTTGGAAGTATCTCTACATTGCTTGAACATCTTGATATAAGCAATTGCAACTTGAGGGGTAACATTCCAAGCGATATTGGAAACTTAAGCAACCTGAGAGTCTTGGACTTGGGAGACAATGAATTCGGTGGGTCAATTCCAACTTCTGTGGGAAGATTAGGGAAGCTTCAAGGTTTGTATTTGAATGATAACAAGTTGCAAGGATACATCCCATATGAACTTTGTCAACTCGACAATGTGGCTGAACTACTTTTGGGTGGTAATCAACTCTCTGGTTCTATACCTTCCTGCTTAGGTAATTTAGCCAAAAGATTTAGAGTTCTATCCTTGCAGTCCAATTTCTTATCTTCCACAATACCATCTACCTTGTGGGAACTGACAGATATCTTGTACCTAAACTTGTCATTTAATTCTCTATTTGGACCTCTCTCAGAAGATATTGGAAAATTGAAAGTTGTGACAATTGTTGATTTGTCAAACAACAATTTATCTGGTATTCTACCAAGTAGCATTGGCCAACTTCAGAATTTGCTTAAACTTTCACTGTCAACTAATGGTATAGAAGGCCCCATTCCCATTACACTTGGAAAGATGACAAGCCTAGAACAGTTGGATTTATCCAAAAACAATCTCTCCGGAGTGATTCCAAAGTCTCTAGAAGCACTCTCTCATCTCAAGAATCTAAATTTGTCTTCCAACAGACTGCAAGGTGAAATTCCAACAGGTGGACCTTTCCAAAACTTCTCTGCCCAGTCATTTGTTTCAAATGCTTTACTCTGTGGTGTCCCCCGCTTGCAAGTTCCACTCTGCAAAAGTCGTACAGACCGCAAACCACATTCAAAGAAAGCTGGTACATCTACCTTGAAATATATTATTCCAGGATTCATTTCAGCAATAATCCTAGTGGCAATTGCTTTAATGTTGATACGACGTAGGAAAAAGAATGTGCAAGTTGTAACAAGTACTACCTGGTCACCTGAATTTTTTTGGAGAAGCATTTCACGCTTAGAACTTGTAAGGGCGACAAATGGATTTCATGAAAGCAACTTACTTGGCGCTGGGGGTTTTGGTTCGGTATATAGAGGTACACTTTCAGATGGGATACATGTTGCAGTAAAGGTTTTCAATTTACAGGTGGAAGGGGCTTTCAAGAGTTATGAAAGGGAATGTGAAATGCTAAGCAATATTCGCCACCGAAATCTTATCAAAATCATTAGCTGTTGCAGTCAAATTGATTTCAAAGCCATGATACTAAACTACATGCCTAATGGGAGCCTCGAGAAGTGGTTGTATTCTCAAAACTCATCTTTGAATATCTTACAGAGGTTGAACATAATGATAGATGTTGCATTGGCATTGGAATACCTACACCATGGTTATTCAATACCTGTTGTCCATTGTGACGTGAAGCCAAGCAATATACTCCTAGATGAAGATATGGTTGCACATGTTGCTGATTTTGGCATTGCAAAGCTAGGTGGAGGAGATTATGTTACCCATACCATGACCCTAGCCACAATTGGATATATGGCACCAGAGTATGGAACAGAAGGAATTGTTTCGACAAGAGGGGACGTGTACAGTTTCGGTATTGTACTCATGGAGACATTTACAAAAAGAAAACCAACAGATGAGATGTTTGTTGAGGAAATGAGTTTAAAGCAATGGGTTTCAAATTCACTATTATCAGATGCATTATCTACAGTTGTGGATGCTAATTTGCTTGGGATGCATCAAGAAGATCATGACTTTGAGACCTGTAATGATTGTTTATCATCCATAATGAGATTATCCCTTGCTTGTGCGGCCGAATCACCGGAAGAGAGGATAAACATGCTAGAAGCTGTATCCACACTCAAGAAAATCAAGATGAAGATTCTGGAGAACGCTGCCAGAGGTGTGCGTTTAAATCTTCCAGTGCTTCCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1187

Amino Acids

130.8

Weight (kDa)

8.53

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 63 - 103 7.4e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 157 - 216 1.5e-06 Leucine rich repeat
LRR_14 PF23598 196 - 258 2.2e-06 Leucine-rich repeat region
LRR_8 PF13855 276 - 336 4.2e-06 Leucine rich repeat
LRR_14 PF23598 297 - 527 4.8e-10 Leucine-rich repeat region
LRR_14 PF23598 553 - 644 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 573 - 633 9.3e-07 Leucine rich repeat
LRR_14 PF23598 648 - 775 5.2e-14 Leucine-rich repeat region
LRR_8 PF13855 696 - 753 2.4e-06 Leucine rich repeat
LRR_8 PF13855 738 - 778 1.7e-06 Leucine rich repeat
Pkinase PF00069 888 - 1159 1e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 891 - 1161 1.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
Acc65I GGTACC 2 cut(s) 437, 1157
AccB1I GGYRCC 3 cut(s) 437, 1157, 3160
AccB7I CCANNNNNTGG 4 cut(s) 261, 1032, 2354, 3170
AccI GTMKAC 2 cut(s) 266, 463
AccIII TCCGGA 2 cut(s) 946, 2264
AciI CCGC 5 cut(s) 145, 309, 2419, 2455, 3434
AcoI YGGCCR 2 cut(s) 2143, 3435
AcuI CTGAAG 3 cut(s) 782, 1063, 2135
AfiI CCNNNNNNNGG 5 cut(s) 261, 383, 1032, 2354, 3170
AflII CTTAAG 1 cut(s) 1714
AflIII ACRYGT 6 cut(s) 33, 786, 891, 2734, 3076, 3204
AhlI ACTAGT 1 cut(s) 230
AjiI CACGTC 2 cut(s) 3034, 3205
AjnI CCWGG 2 cut(s) 2506, 2597
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AleI CACNNNNGTG 1 cut(s) 335
Alw26I GTCTC 8 cut(s) 362, 462, 702, 1475, 1737, 2286, 3227, 3377
Ama87I CYCGRG 1 cut(s) 2906
Aor13HI TCCGGA 2 cut(s) 946, 2264
AoxI GGCC 4 cut(s) 1228, 2143, 2194, 3435
ApeKI GCWGC 3 cut(s) 170, 200, 3518
ArsI GACNNNNNNTTYG 2 cut(s) 1736, 1768
AseI ATTAAT 1 cut(s) 155
Asp700I GAANNNNTTC 4 cut(s) 91, 798, 2626, 3187
Asp718I GGTACC 2 cut(s) 437, 1157
AspA2I CCTAGG 1 cut(s) 382
AspLEI GCGC 1 cut(s) 2687
AspS9I GGNCC 7 cut(s) 866, 1013, 1229, 1330, 2051, 2195, 2357
AsuHPI GGTGA 4 cut(s) 371, 2351, 2595, 3435
AsuII TTCGAA 1 cut(s) 792
AvaI CYCGRG 1 cut(s) 2906
AvaII GGWCC 5 cut(s) 866, 1013, 1330, 2051, 2357
AvrII CCTAGG 1 cut(s) 382
BalI TGGCCA 1 cut(s) 2145
BanI GGYRCC 3 cut(s) 437, 1157, 3160
BanII GRGCYC 1 cut(s) 534
BarI GAAGNNNNNNTAC 4 cut(s) 755, 787, 1627, 1659
BbsI GAAGAC 4 cut(s) 34, 877, 888, 2313
BbvI GCAGC 3 cut(s) 182, 187, 3505
BccI CCATC 8 cut(s) 526, 605, 1004, 1030, 1241, 1246, 1996, 2720
BciT130I CCWGG 2 cut(s) 2508, 2599
BciVI GTATCC 4 cut(s) 986, 1826, 2724, 3487
BcoDI GTCTC 8 cut(s) 362, 462, 702, 1475, 1737, 2286, 3227, 3377
BcuI ACTAGT 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 3330
BfoI RGCGCY 1 cut(s) 2688
BfrI CTTAAG 1 cut(s) 1714
BfuI GTATCC 4 cut(s) 986, 1826, 2724, 3487
BglII AGATCT 1 cut(s) 981
BisI GCNGC 4 cut(s) 171, 201, 3435, 3519
BlnI CCTAGG 1 cut(s) 382
BlpI GCTNAGC 1 cut(s) 2807
BlsI GCNGC 4 cut(s) 172, 202, 3436, 3520
BmcAI AGTACT 2 cut(s) 624, 2593
Bme1390I CCNGG 2 cut(s) 2508, 2599
Bme18I GGWCC 5 cut(s) 866, 1013, 1330, 2051, 2357
BmeT110I CYCGRG 1 cut(s) 2906
BmgBI CACGTC 2 cut(s) 3034, 3205
BmgT120I GGNCC 7 cut(s) 866, 1013, 1229, 1330, 2051, 2195, 2357
BmiI GGNNCC 8 cut(s) 439, 1159, 1231, 1331, 1332, 2197, 2903, 3162
BmrFI CCNGG 2 cut(s) 2508, 2599
BmrI ACTGGG 3 cut(s) 301, 1111, 2374
BmsI GCATC 6 cut(s) 973, 1110, 3310, 3331, 3348, 3370
BmuI ACTGGG 3 cut(s) 301, 1111, 2374
BpiI GAAGAC 4 cut(s) 34, 877, 888, 2313
BplI GAGNNNNNCTC 2 cut(s) 979, 1011
BpmI CTGGAG 4 cut(s) 1037, 1395, 1518, 3530
Bpu10I CCTNAGC 3 cut(s) 125, 1498, 1921
Bpu1102I GCTNAGC 1 cut(s) 2807
Bpu14I TTCGAA 1 cut(s) 792
BpuEI CTTGAG 5 cut(s) 1006, 1505, 1702, 2288, 3470
BsaAI YACGTR 2 cut(s) 36, 844
BsaI GGTCTC 2 cut(s) 362, 3377
BsaJI CCNNGG 4 cut(s) 382, 433, 1225, 3001
BsaWI WCCGGW 4 cut(s) 946, 1621, 2264, 3445
BsaXI ACNNNNNCTCC 8 cut(s) 824, 854, 1516, 1546, 2246, 2276, 3105, 3135
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 383, 1032, 2354, 3170
Bse1I ACTGG 8 cut(s) 266, 296, 940, 1054, 1117, 1378, 2380, 3545
Bse3DI GCAATG 4 cut(s) 1043, 1646, 3093, 3299
BseAI TCCGGA 2 cut(s) 946, 2264
BseBI CCWGG 2 cut(s) 2508, 2599
BseDI CCNNGG 4 cut(s) 382, 433, 1225, 3001
BseGI GGATG 8 cut(s) 1022, 1233, 1238, 1545, 1836, 3346, 3363, 3404
BseLI CCNNNNNNNGG 5 cut(s) 261, 383, 1032, 2354, 3170
BseMI GCAATG 4 cut(s) 1043, 1646, 3093, 3299
BseMII CTCAG 5 cut(s) 116, 373, 1311, 1715, 2073
BseNI ACTGG 8 cut(s) 266, 296, 940, 1054, 1117, 1378, 2380, 3545
BseRI GAGGAG 2 cut(s) 366, 3126
BseX3I CGGCCG 1 cut(s) 3435
BseXI GCAGC 3 cut(s) 182, 187, 3505
BseYI CCCAGC 1 cut(s) 2687
BsgI GTGCAG 2 cut(s) 597, 866
Bsh1285I CGRYCG 1 cut(s) 3438
BshFI GGCC 4 cut(s) 1230, 2145, 2196, 3437
BshNI GGYRCC 3 cut(s) 437, 1157, 3160
BsiEI CGRYCG 1 cut(s) 3438
BsiHKCI CYCGRG 1 cut(s) 2906
BsiSI CCGG 4 cut(s) 947, 1622, 2265, 3446
BslFI GGGAC 3 cut(s) 1316, 2399, 3215
BslI CCNNNNNNNGG 5 cut(s) 261, 383, 1032, 2354, 3170
BsmAI GTCTC 8 cut(s) 362, 462, 702, 1475, 1737, 2286, 3227, 3377
BsmFI GGGAC 3 cut(s) 1316, 2399, 3215
BsmI GAATGC 2 cut(s) 598, 942
BsnI GGCC 4 cut(s) 1230, 2145, 2196, 3437
Bso31I GGTCTC 2 cut(s) 362, 3377
BsoBI CYCGRG 1 cut(s) 2906
Bsp119I TTCGAA 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 534
Bsp13I TCCGGA 2 cut(s) 946, 2264
Bsp1407I TGTACA 1 cut(s) 3207
Bsp143I GATC 5 cut(s) 628, 823, 981, 1263, 3370
Bsp1720I GCTNAGC 1 cut(s) 2807
Bsp19I CCATGG 3 cut(s) 433, 1225, 3001
BspACI CCGC 5 cut(s) 145, 309, 2419, 2455, 3434
BspANI GGCC 4 cut(s) 1230, 2145, 2196, 3437
BspCNI CTCAG 5 cut(s) 117, 372, 1310, 1716, 2072
BspEI TCCGGA 2 cut(s) 946, 2264
BspHI TCATGA 2 cut(s) 2665, 3373
BspLI GGNNCC 8 cut(s) 439, 1159, 1231, 1331, 1332, 2197, 2903, 3162
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 792
BspT107I GGYRCC 3 cut(s) 437, 1157, 3160
BspTI CTTAAG 1 cut(s) 1714
BspTNI GGTCTC 2 cut(s) 362, 3377
BsrDI GCAATG 4 cut(s) 1043, 1646, 3093, 3299
BsrGI TGTACA 1 cut(s) 3207
BsrI ACTGG 8 cut(s) 266, 296, 940, 1054, 1117, 1378, 2380, 3545
BssECI CCNNGG 4 cut(s) 382, 433, 1225, 3001
BssMI GATC 5 cut(s) 628, 823, 981, 1263, 3370
BssT1I CCWWGG 4 cut(s) 382, 433, 1225, 3001
Bst2UI CCWGG 2 cut(s) 2508, 2599
Bst4CI ACNGT 7 cut(s) 166, 337, 428, 2175, 2238, 3212, 3334
Bst6I CTCTTC 4 cut(s) 210, 1284, 1623, 3444
BstAFI CTTAAG 1 cut(s) 1714
BstAPI GCANNNNNTGC 2 cut(s) 306, 3080
BstAUI TGTACA 1 cut(s) 3207
BstBAI YACGTR 2 cut(s) 36, 844
BstBI TTCGAA 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 849, 997, 2423
BstDSI CCRYGG 3 cut(s) 433, 1225, 3001
BstEII GGTNACC 1 cut(s) 2601
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 8 cut(s) 1022, 1233, 1238, 1545, 1836, 3346, 3363, 3404
BstH2I RGCGCY 1 cut(s) 2688
BstHHI GCGC 1 cut(s) 2687
BstKTI GATC 5 cut(s) 631, 826, 984, 1266, 3373
BstMAI GTCTC 8 cut(s) 362, 462, 702, 1475, 1737, 2286, 3227, 3377
BstMBI GATC 5 cut(s) 628, 823, 981, 1263, 3370
BstMCI CGRYCG 1 cut(s) 3438
BstNI CCWGG 2 cut(s) 2508, 2599
BstNSI RCATGY 7 cut(s) 790, 895, 1214, 2738, 2893, 3080, 3466
BstPI GGTNACC 1 cut(s) 2601
BstSCI CCNGG 2 cut(s) 2506, 2597
BstSFI CTRYAG 1 cut(s) 3330
BstV1I GCAGC 3 cut(s) 182, 187, 3505
BstV2I GAAGAC 4 cut(s) 34, 877, 888, 2313
BstX2I RGATCY 1 cut(s) 981
BstXI CCANNNNNNTGG 1 cut(s) 650
BstYI RGATCY 1 cut(s) 981
BstZI CGGCCG 1 cut(s) 3435
BsuI GTATCC 4 cut(s) 986, 1826, 2724, 3487
BsuRI GGCC 4 cut(s) 1230, 2145, 2196, 3437
BtgI CCRYGG 3 cut(s) 433, 1225, 3001
BtrI CACGTC 2 cut(s) 3034, 3205
BtsCI GGATG 8 cut(s) 1022, 1233, 1238, 1545, 1836, 3346, 3363, 3404
BtsI GCAGTG 2 cut(s) 614, 1205
BtsIMutI CAGTG 6 cut(s) 614, 1087, 1205, 1371, 2171, 3552
Cac8I GCNNGC 3 cut(s) 849, 997, 2423
CciI TCATGA 2 cut(s) 2665, 3373
CfoI GCGC 1 cut(s) 2687
Cfr13I GGNCC 7 cut(s) 866, 1013, 1229, 1330, 2051, 2195, 2357
CsiI ACCWGGT 1 cut(s) 2597
CspCI CAANNNNNGTGG 6 cut(s) 435, 470, 1063, 1098, 1758, 1793
DpnI GATC 5 cut(s) 630, 825, 983, 1265, 3372
DpnII GATC 5 cut(s) 628, 823, 981, 1263, 3370
DraI TTTAAA 2 cut(s) 3288, 3537
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 2143, 3435
EagI CGGCCG 1 cut(s) 3435
Eam1104I CTCTTC 4 cut(s) 210, 1284, 1623, 3444
EarI CTCTTC 4 cut(s) 210, 1284, 1623, 3444
EclXI CGGCCG 1 cut(s) 3435
Eco130I CCWWGG 4 cut(s) 382, 433, 1225, 3001
Eco24I GRGCYC 1 cut(s) 534
Eco31I GGTCTC 2 cut(s) 362, 3377
Eco32I GATATC 2 cut(s) 699, 2014
Eco47I GGWCC 5 cut(s) 866, 1013, 1330, 2051, 2357
Eco52I CGGCCG 1 cut(s) 3435
Eco57I CTGAAG 3 cut(s) 782, 1063, 2135
Eco88I CYCGRG 1 cut(s) 2906
Eco91I GGTNACC 1 cut(s) 2601
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoO109I RGGNCCY 3 cut(s) 866, 1330, 2195
EcoO65I GGTNACC 1 cut(s) 2601
EcoRI GAATTC 3 cut(s) 274, 413, 1751
EcoRII CCWGG 2 cut(s) 2506, 2597
EcoRV GATATC 2 cut(s) 699, 2014
EcoT14I CCWWGG 4 cut(s) 382, 433, 1225, 3001
EcoT22I ATGCAT 2 cut(s) 3325, 3363
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 4 cut(s) 382, 433, 1225, 3001
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 3 cut(s) 1316, 2399, 3215
FauI CCCGC 1 cut(s) 2426
FauNDI CATATG 1 cut(s) 1843
FblI GTMKAC 2 cut(s) 266, 463
Fnu4HI GCNGC 4 cut(s) 171, 201, 3435, 3519
FokI GGATG 8 cut(s) 1009, 1220, 1225, 1532, 1823, 3353, 3370, 3391
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 4 cut(s) 171, 201, 3435, 3519
GlaI GCGC 1 cut(s) 2686
GluI GCNGC 4 cut(s) 171, 201, 3435, 3519
GsaI CCCAGC 1 cut(s) 2691
GsuI CTGGAG 4 cut(s) 1037, 1395, 1518, 3530
HaeII RGCGCY 1 cut(s) 2688
HaeIII GGCC 4 cut(s) 1230, 2145, 2196, 3437
HapII CCGG 4 cut(s) 947, 1622, 2265, 3446
HhaI GCGC 1 cut(s) 2687
Hin6I GCGC 1 cut(s) 2685
HinP1I GCGC 1 cut(s) 2685
HincII GTYRAC 2 cut(s) 1856, 2178
HindII GTYRAC 2 cut(s) 1856, 2178
HindIII AAGCTT 1 cut(s) 1793
HinfI GANTC 9 cut(s) 327, 340, 1584, 1728, 2272, 2308, 2511, 3440, 3505
HpaII CCGG 4 cut(s) 947, 1622, 2265, 3446
HphI GGTGA 4 cut(s) 371, 2351, 2595, 3435
Hpy166II GTNNAC 9 cut(s) 267, 464, 1016, 1543, 1856, 2178, 2357, 2717, 3208
Hpy188I TCNGA 8 cut(s) 56, 362, 419, 541, 2062, 2154, 2725, 3319
Hpy8I GTNNAC 9 cut(s) 267, 464, 1016, 1543, 1856, 2178, 2357, 2717, 3208
Hpy99I CGWCG 1 cut(s) 2562
HpyAV CCTTC 8 cut(s) 562, 685, 1316, 1560, 1923, 2186, 2762, 3173
HpyCH4III ACNGT 7 cut(s) 166, 337, 428, 2175, 2238, 3212, 3334
HpyCH4IV ACGT 5 cut(s) 35, 843, 2560, 3033, 3204
HpySE526I ACGT 5 cut(s) 35, 843, 2560, 3033, 3204
HspAI GCGC 1 cut(s) 2685
KflI GGGWCCC 1 cut(s) 1330
Kpn2I TCCGGA 2 cut(s) 946, 2264
KpnI GGTACC 2 cut(s) 441, 1161
Kzo9I GATC 5 cut(s) 628, 823, 981, 1263, 3370
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 1376, 2901
Lsp1109I GCAGC 3 cut(s) 182, 187, 3505
LweI GCATC 6 cut(s) 973, 1110, 3310, 3331, 3348, 3370
MabI ACCWGGT 1 cut(s) 2597
MaeII ACGT 5 cut(s) 35, 843, 2560, 3033, 3204
MaeIII GTNAC 9 cut(s) 233, 328, 1203, 1688, 2086, 2584, 2601, 3029, 3121
MalI GATC 5 cut(s) 630, 825, 983, 1265, 3372
MboI GATC 5 cut(s) 628, 823, 981, 1263, 3370
MfeI CAATTG 4 cut(s) 1672, 2091, 2538, 3147
MflI RGATCY 1 cut(s) 981
MhlI GDGCHC 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 2145
MluNI TGGCCA 1 cut(s) 2145
MlyI GAGTC 2 cut(s) 336, 1737
MmeI TCCRAC 5 cut(s) 1481, 1793, 2220, 2349, 2372
Mox20I TGGCCA 1 cut(s) 2145
Mph1103I ATGCAT 2 cut(s) 3325, 3363
MroI TCCGGA 2 cut(s) 946, 2264
MroXI GAANNNNTTC 4 cut(s) 91, 798, 2626, 3187
MscI TGGCCA 1 cut(s) 2145
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 2145
MspA1I CMGCKG 1 cut(s) 409
MspCI CTTAAG 1 cut(s) 1714
MspI CCGG 4 cut(s) 947, 1622, 2265, 3446
MspR9I CCNGG 2 cut(s) 2508, 2599
MunI CAATTG 4 cut(s) 1672, 2091, 2538, 3147
Mva1269I GAATGC 2 cut(s) 598, 942
MvaI CCWGG 2 cut(s) 2508, 2599
NcoI CCATGG 3 cut(s) 433, 1225, 3001
NdeI CATATG 1 cut(s) 1843
NdeII GATC 5 cut(s) 628, 823, 981, 1263, 3370
NlaIV GGNNCC 8 cut(s) 439, 1159, 1231, 1331, 1332, 2197, 2903, 3162
NmuCI GTSAC 6 cut(s) 233, 328, 1203, 2086, 2601, 3029
NsiI ATGCAT 2 cut(s) 3325, 3363
NspI RCATGY 7 cut(s) 790, 895, 1214, 2738, 2893, 3080, 3466
NspV TTCGAA 1 cut(s) 792
OliI CACNNNNGTG 1 cut(s) 335
PaeR7I CTCGAG 1 cut(s) 2906
PagI TCATGA 2 cut(s) 2665, 3373
PciI ACATGT 4 cut(s) 786, 891, 2734, 3076
PciSI GCTCTTC 1 cut(s) 210
PctI GAATGC 2 cut(s) 598, 942
PdmI GAANNNNTTC 4 cut(s) 91, 798, 2626, 3187
PfeI GAWTC 7 cut(s) 340, 1584, 2272, 2308, 2511, 3440, 3505
PflMI CCANNNNNTGG 4 cut(s) 261, 1032, 2354, 3170
PfoI TCCNGGA 1 cut(s) 2506
PkrI GCNGC 4 cut(s) 172, 202, 3436, 3520
PleI GAGTC 2 cut(s) 335, 1736
PpsI GAGTC 2 cut(s) 335, 1736
Ppu21I YACGTR 2 cut(s) 36, 844
PpuMI RGGWCCY 2 cut(s) 866, 1330
PscI ACATGT 4 cut(s) 786, 891, 2734, 3076
PshBI ATTAAT 1 cut(s) 155
Psp5II RGGWCCY 2 cut(s) 866, 1330
Psp6I CCWGG 2 cut(s) 2506, 2597
PspEI GGTNACC 1 cut(s) 2601
PspFI CCCAGC 1 cut(s) 2687
PspGI CCWGG 2 cut(s) 2506, 2597
PspN4I GGNNCC 8 cut(s) 439, 1159, 1231, 1331, 1332, 2197, 2903, 3162
PspPI GGNCC 7 cut(s) 866, 1013, 1229, 1330, 2051, 2195, 2357
PspPPI RGGWCCY 2 cut(s) 866, 1330
PsuI RGATCY 1 cut(s) 981
PvuII CAGCTG 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 210
SatI GCNGC 4 cut(s) 171, 201, 3435, 3519
Sau3AI GATC 5 cut(s) 628, 823, 981, 1263, 3370
Sau96I GGNCC 7 cut(s) 866, 1013, 1229, 1330, 2051, 2195, 2357
ScaI AGTACT 2 cut(s) 624, 2593
SchI GAGTC 2 cut(s) 336, 1737
ScrFI CCNGG 2 cut(s) 2508, 2599
SduI GDGCHC 1 cut(s) 534
SexAI ACCWGGT 1 cut(s) 2597
SfaNI GCATC 6 cut(s) 973, 1110, 3310, 3331, 3348, 3370
SfcI CTRYAG 1 cut(s) 3330
Sfr274I CTCGAG 1 cut(s) 2906
SfuI TTCGAA 1 cut(s) 792
SinI GGWCC 5 cut(s) 866, 1013, 1330, 2051, 2357
SlaI CTCGAG 1 cut(s) 2906
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 7 cut(s) 985, 1484, 1681, 1714, 2303, 2906, 3485
SmoI CTYRAG 7 cut(s) 985, 1484, 1681, 1714, 2303, 2906, 3485
SpeI ACTAGT 1 cut(s) 230
SsiI CCGC 5 cut(s) 145, 309, 2419, 2455, 3434
SspI AATATT 2 cut(s) 1248, 2815
StyD4I CCNGG 2 cut(s) 2506, 2597
StyI CCWWGG 4 cut(s) 382, 433, 1225, 3001
TaaI ACNGT 7 cut(s) 166, 337, 428, 2175, 2238, 3212, 3334
TaiI ACGT 5 cut(s) 38, 846, 2563, 3036, 3207
TaqI TCGA 6 cut(s) 104, 555, 792, 1860, 2907, 3191
TatI WGTACW 4 cut(s) 622, 2591, 3207, 3223
TauI GCSGC 1 cut(s) 3437
TfiI GAWTC 7 cut(s) 340, 1584, 2272, 2308, 2511, 3440, 3505
TscAI CASTG 6 cut(s) 621, 1087, 1212, 1378, 2178, 3552
TseFI GTSAC 6 cut(s) 233, 328, 1203, 2086, 2601, 3029
TseI GCWGC 3 cut(s) 170, 200, 3518
Tsp45I GTSAC 6 cut(s) 233, 328, 1203, 2086, 2601, 3029
TspGWI ACGGA 1 cut(s) 481
TspRI CASTG 6 cut(s) 621, 1087, 1212, 1378, 2178, 3552
Van91I CCANNNNNTGG 4 cut(s) 261, 1032, 2354, 3170
Vha464I CTTAAG 1 cut(s) 1714
VpaK11BI GGWCC 5 cut(s) 866, 1013, 1330, 2051, 2357
VspI ATTAAT 1 cut(s) 155
XagI CCTNNNNNAGG 1 cut(s) 381
XbaI TCTAGA 2 cut(s) 1420, 2284
XceI RCATGY 7 cut(s) 790, 895, 1214, 2738, 2893, 3080, 3466
XcmI CCANNNNNNNNNTGG 2 cut(s) 1029, 1995
XhoI CTCGAG 1 cut(s) 2906
XmaJI CCTAGG 1 cut(s) 382
XmiI GTMKAC 2 cut(s) 266, 463
XmnI GAANNNNTTC 4 cut(s) 91, 798, 2626, 3187
ZrmI AGTACT 2 cut(s) 624, 2593
Zsp2I ATGCAT 2 cut(s) 3325, 3363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.