Rmu_sc0003539.1_g000012

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003539.1
OG0000058
Physical Location & Seq
Forward (+)
46221 .. 50057
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003539.1_g000012.1.cds

Sequence Viewer

Length: 3288 bp
atggcagcacatgtacaaaccaacatcagcatagaccagtctgctcttcttgctttcaaagccagtatcaccgatgatcctcataaactgatcttcaccaactggtcaaccatcgccccagtttgtaactgggttggcgttacttgtgctgctcgccaccacagagttgcggtcttgaacttatcttattttggtctcactggcaccattcctccggagctaggaaacctttcatttcttgtggaacttcgcctcagaaataacagttttcatggtaccttgcctcaggaattggctagcctgcaccggttgaggttgattagcttgggatataacaagtttatgggaaccattcccacatggttcgggtccttatccaaacttcaaacatttaatttgtatggtaatcagttttcaggttccataccagccaccatcttgaacttatctgcacttcaagtaattgatctgacgtataaccaactctcaggtaacataccaagacagattggaaacttaacaatgttgaaggaaatatacctggacagtaataaattcacagaaattcctaatgagattggcggtttagatcaattggaaatattgtttgtgcaattgaatgctttaaaagggtctattcctatggttgttttcaacatgtcttctttgactatgttggctctatatggaaacaatttaaatggtagtcttccggacaatatatgtcaacatcttccgagtattcaagatttgagtctgggttacaaccaattcgacggtccacttccatcgaaattgtgggaatgcaaagaactccttcaattggaagtaccacgcaacagtttcagtggagaaataccaaatacttttgggaacttgactcagatacagacaattgaccttggcttgaactatttgacaggtcctataccacatcagattggtcatcttccaaatttagagatattgaacctcggtgggaataacttcaatgaggtcatcccatcctcaatcttcaatatgtccacaatagcagtgatatcgcttttcgagaatcagctctcaggcaaccttccaccaaacacaggccttggccttccaaacctacaacgcttctatctaggaggaaataaacttagcggagcaatccccaaatctttgtccaatgcttctaacctggagctcatagacctaggcagcaacctattatttggcgttattcctagcaccctatgtaccttgacaaaacttcagctgcttcaactatttgataataacctgaccgtcagtacttcgactccagaactggatgttctcttttgtttggcaaatcttcgcaacttgaggagactagacttctcagtgaatccattagatgccaaccttccgatttcctttacgaatacatctcttcaatatatttatttttacaactgcaacatgaggggtgaaattcccaatgacatcggcaacttgagcagcttgatagtattatctgtgctaaagaatcaattaagtggattggttccagcctcaataggaagaatccgaaacctccaaggtctatatttggaaagtaacacatttcaaggacgcatgccagatgaactctgccaactgaaaaacctaggttctttgtttttgggtggcaatcgactgtctggctctataccttcatgcatcggcaatctaactggagctctaaggaaactatcattggagtctaatttgttaacttctacagtaccatctaccttgtggggacttacatatatcctgtacttgaacttgtcatcgaattctctgatcgggtctatcggagaaggtgttggtaatttgaaagttgccacaggtatagatttttcaaacaaccgattatccggtagtatagcaagcagcgttggtggacttcaaaacttggtcaatctgtccttggcaaataataaactacaaggctccattcctacttcacttggcaattgcataagcctggaatctttggacttatccaaaaacaacttatcaggagtgattcccaactctctttcagcgctctcatatctgaagtatttgaatttttctttcaacagactccaaggagaaattccaacaggtggtcctttcagaaacttgtctactcaatcatttgtttccaaccatggactctgtggtgcagcccgatttcaagttccatcatgcaaaaggaagactgttaaaactattttcaagtatgttattccgggtatcttgtccgcaatgcttctagtgacctccatttggatatttattaaatcgagaaagaagaatgtgaaagctgcaacagagactacctttttgccacaacatcagtggagaagagtttcctatgaagagcttcttagtgcaacaagtggattcaatgtgagcaacttacttggcactgggagttttggctcagtatataaaggaacactgtcagatgggatagatgttgccattaaggtatataatttagacacagaaggggcattctcaagttttgatgtcgaatgcgaaatgctaagcaatattcgccatcgaaaccttatcaaagtcatcagttgctgcagtcaagttgatttcaaagccgttgtactaaactacatgcctaatgggagccttgaaaagtggttgtattctcataacttaaccttgaacatcttgcagaggttgaatatattgatagatgttgcaacggcattggagtatctccatcatggttatgatagacctattgtgcattgtgatctgaagccaagcaacatactactagatgatgatatggttgcacatgttgctgattttggtatcgcaaaactcttgggtggagaggattctatcactcgaaccttgacccttgccacaatcgggtacatggctccagagtatggaatggatggaatagtgaccagaagaggggatatgtatagttttggtgttgtagtgatggagacattcacaaaaaggaagccaacagatgcgatgtttgtgggggaaatgaatttaaagcactgggttgcaaattcacttcttgcagatgcaatagttgaagttgttgataccactttacttgggacagaggaatatcatgactttgtgagcaagagagattgtttatcatctattatgagattgggtattgcttgttgtggagaatcaccagaagacaggataaatatgcacgaggctctagccacgctgaacaaaatcaagatcaaatttttgaagaacgcttcagaaggtgtggtattgagccgtcatggcctctga

Protein Analysis

1095

Amino Acids

119.9

Weight (kDa)

6.87

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 203, 275
AccB7I CCANNNNNTGG 2 cut(s) 2114, 2906
AccI GTMKAC 1 cut(s) 2135
AccIII TCCGGA 2 cut(s) 214, 712
AciI CCGC 4 cut(s) 170, 582, 1140, 2253
AclWI GGATC 1 cut(s) 71
AcuI CTGAAG 4 cut(s) 1235, 2084, 2789, 3237
AfaI GTAC 9 cut(s) 15, 277, 831, 1237, 1291, 1746, 1781, 2613, 2891
AfeI AGCGCT 1 cut(s) 2052
AfiI CCNNNNNNNGG 8 cut(s) 221, 823, 1085, 2114, 2277, 2886, 2906, 2934
AflIII ACRYGT 3 cut(s) 10, 657, 2809
AgeI ACCGGT 1 cut(s) 306
AjnI CCWGG 3 cut(s) 540, 1176, 1989
AjuI GAANNNNNNNTTGG 4 cut(s) 1700, 1732, 2088, 2120
AluBI AGCT 9 cut(s) 220, 324, 1060, 1183, 1255, 1482, 1700, 2315, 2374
AluI AGCT 9 cut(s) 220, 324, 1060, 1183, 1255, 1482, 1700, 2315, 2374
Alw21I GWGCWC 2 cut(s) 1185, 1702
Alw26I GTCTC 4 cut(s) 200, 1342, 2318, 2963
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 2583
Aor13HI TCCGGA 2 cut(s) 214, 712
Aor51HI AGCGCT 1 cut(s) 2052
AoxI GGCC 3 cut(s) 1087, 1093, 3280
ApeKI GCWGC 9 cut(s) 5, 149, 1197, 1255, 1479, 1896, 2174, 2315, 2583
ArsI GACNNNNNNTTYG 2 cut(s) 2317, 2349
AsiGI ACCGGT 1 cut(s) 306
Asp700I GAANNNNTTC 5 cut(s) 229, 816, 986, 2359, 2373
Asp718I GGTACC 1 cut(s) 275
AspA2I CCTAGG 2 cut(s) 1192, 1627
AspLEI GCGC 1 cut(s) 2053
AspS9I GGNCC 4 cut(s) 369, 779, 923, 2117
AsuC2I CCSGG 1 cut(s) 2241
AsuHPI GGTGA 4 cut(s) 61, 88, 1460, 3168
AsuNHI GCTAGC 1 cut(s) 296
AvaII GGWCC 4 cut(s) 369, 779, 923, 2117
AvrII CCTAGG 2 cut(s) 1192, 1627
AxyI CCTNAGG 1 cut(s) 285
BanI GGYRCC 2 cut(s) 203, 275
BanII GRGCYC 2 cut(s) 1185, 1702
BarI GAAGNNNNNNTAC 2 cut(s) 521, 553
BauI CACGAG 1 cut(s) 3200
BbsI GAAGAC 4 cut(s) 654, 701, 2213, 3189
Bbv12I GWGCWC 2 cut(s) 1185, 1702
BbvI GCAGC 9 cut(s) 17, 136, 1209, 1242, 1491, 1908, 2186, 2302, 2570
BceAI ACGGC 3 cut(s) 2591, 2730, 3258
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BciT130I CCWGG 3 cut(s) 542, 1178, 1991
BcnI CCSGG 1 cut(s) 2241
BcoDI GTCTC 4 cut(s) 200, 1342, 2318, 2963
BfmI CTRYAG 2 cut(s) 1740, 2584
BfoI RGCGCY 1 cut(s) 2054
BglI GCCNNNNNGGC 1 cut(s) 3279
BisI GCNGC 9 cut(s) 6, 150, 1198, 1256, 1480, 1897, 2175, 2316, 2584
BlnI CCTAGG 2 cut(s) 1192, 1627
BlpI GCTNAGC 1 cut(s) 2540
BlsI GCNGC 9 cut(s) 7, 151, 1199, 1257, 1481, 1898, 2176, 2317, 2585
BmcAI AGTACT 1 cut(s) 1291
Bme1390I CCNGG 4 cut(s) 542, 1178, 1991, 2241
Bme18I GGWCC 4 cut(s) 369, 779, 923, 2117
BmgT120I GGNCC 4 cut(s) 369, 779, 923, 2117
BmiI GGNNCC 9 cut(s) 205, 277, 349, 370, 421, 1527, 1957, 2636, 2898
BmrFI CCNGG 4 cut(s) 542, 1178, 1991, 2241
BmrI ACTGGG 4 cut(s) 113, 139, 2430, 3040
BmsI GCATC 4 cut(s) 1366, 1689, 2986, 3046
BmtI GCTAGC 1 cut(s) 300
BmuI ACTGGG 4 cut(s) 113, 139, 2430, 3040
BpiI GAAGAC 4 cut(s) 654, 701, 2213, 3189
BpmI CTGGAG 4 cut(s) 1199, 1284, 1716, 2883
Bpu1102I GCTNAGC 1 cut(s) 2540
BpuEI CTTGAG 3 cut(s) 1363, 1495, 2497
BpuMI CCSGG 1 cut(s) 2241
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 9 cut(s) 901, 973, 1090, 1192, 1558, 1627, 1932, 2095, 2158
BsaWI WCCGGW 4 cut(s) 214, 306, 712, 1880
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 1282, 1312, 1812, 1842
Bsc4I CCNNNNNNNGG 8 cut(s) 221, 823, 1085, 2114, 2277, 2886, 2906, 2934
Bse118I RCCGGY 1 cut(s) 306
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 2262
BseAI TCCGGA 2 cut(s) 214, 712
BseBI CCWGG 3 cut(s) 542, 1178, 1991
BseDI CCNNGG 9 cut(s) 901, 973, 1090, 1192, 1558, 1627, 1932, 2095, 2158
BseGI GGATG 4 cut(s) 999, 1004, 1315, 2920
BseLI CCNNNNNNNGG 8 cut(s) 221, 823, 1085, 2114, 2277, 2886, 2906, 2934
BseMI GCAATG 1 cut(s) 2262
BseMII CTCAG 7 cut(s) 268, 299, 501, 896, 1077, 1374, 2448
BseRI GAGGAG 1 cut(s) 1360
BseXI GCAGC 9 cut(s) 17, 136, 1209, 1242, 1491, 1908, 2186, 2302, 2570
BsgI GTGCAG 3 cut(s) 287, 435, 2193
BshFI GGCC 3 cut(s) 1089, 1095, 3282
BshNI GGYRCC 2 cut(s) 203, 275
BshTI ACCGGT 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 1185, 1702
BsiSI CCGG 5 cut(s) 215, 307, 713, 1881, 2240
BslFI GGGAC 2 cut(s) 1776, 3106
BslI CCNNNNNNNGG 8 cut(s) 221, 823, 1085, 2114, 2277, 2886, 2906, 2934
BsmAI GTCTC 4 cut(s) 200, 1342, 2318, 2963
BsmFI GGGAC 2 cut(s) 1776, 3106
BsmI GAATGC 4 cut(s) 625, 809, 2507, 2534
BsnI GGCC 3 cut(s) 1089, 1095, 3282
Bso31I GGTCTC 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 1185, 1702
Bsp13I TCCGGA 2 cut(s) 214, 712
Bsp1407I TGTACA 1 cut(s) 13
Bsp143I GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
Bsp1720I GCTNAGC 1 cut(s) 2540
Bsp19I CCATGG 1 cut(s) 2158
BspACI CCGC 4 cut(s) 170, 582, 1140, 2253
BspANI GGCC 3 cut(s) 1089, 1095, 3282
BspCNI CTCAG 7 cut(s) 267, 298, 500, 895, 1076, 1373, 2447
BspEI TCCGGA 2 cut(s) 214, 712
BspHI TCATGA 1 cut(s) 3106
BspLI GGNNCC 9 cut(s) 205, 277, 349, 370, 421, 1527, 1957, 2636, 2898
BspMAI CTGCAG 1 cut(s) 2588
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 2 cut(s) 51, 2364
BspT107I GGYRCC 2 cut(s) 203, 275
BspTNI GGTCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 2262
BsrFI RCCGGY 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 306
BssECI CCNNGG 9 cut(s) 901, 973, 1090, 1192, 1558, 1627, 1932, 2095, 2158
BssMI GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
BssSI CACGAG 1 cut(s) 3200
BssT1I CCWWGG 8 cut(s) 901, 1090, 1192, 1558, 1627, 1932, 2095, 2158
Bst2BI CACGAG 1 cut(s) 3200
Bst2UI CCWGG 3 cut(s) 542, 1178, 1991
Bst4CI ACNGT 9 cut(s) 266, 548, 779, 842, 1285, 1659, 1744, 2212, 2454
Bst6I CTCTTC 5 cut(s) 51, 1416, 2350, 2364, 2926
BstAPI GCANNNNNTGC 1 cut(s) 2813
BstAUI TGTACA 1 cut(s) 13
BstC8I GCNNGC 5 cut(s) 154, 298, 302, 1598, 1894
BstDSI CCRYGG 1 cut(s) 2158
BstF5I GGATG 4 cut(s) 999, 1004, 1315, 2920
BstH2I RGCGCY 1 cut(s) 2054
BstHHI GCGC 1 cut(s) 2053
BstKTI GATC 7 cut(s) 79, 93, 469, 592, 1809, 2767, 3234
BstMAI GTCTC 4 cut(s) 200, 1342, 2318, 2963
BstMBI GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
BstMWI GCNNNNNNNGC 6 cut(s) 50, 59, 1476, 2550, 2813, 3279
BstNI CCWGG 3 cut(s) 542, 1178, 1991
BstNSI RCATGY 5 cut(s) 14, 661, 1600, 2626, 2813
BstSCI CCNGG 4 cut(s) 540, 1176, 1989, 2239
BstSFI CTRYAG 2 cut(s) 1740, 2584
BstV1I GCAGC 9 cut(s) 17, 136, 1209, 1242, 1491, 1908, 2186, 2302, 2570
BstV2I GAAGAC 4 cut(s) 654, 701, 2213, 3189
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 1089, 1095, 3282
BtgI CCRYGG 1 cut(s) 2158
BtgZI GCGATG 2 cut(s) 97, 3014
BtsCI GGATG 4 cut(s) 999, 1004, 1315, 2920
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 8 cut(s) 198, 853, 1041, 1368, 2354, 2418, 2450, 3028
Cac8I GCNNGC 5 cut(s) 154, 298, 302, 1598, 1894
CaiI CAGNNNCTG 1 cut(s) 2583
CciI TCATGA 1 cut(s) 3106
CfoI GCGC 1 cut(s) 2053
Cfr10I RCCGGY 1 cut(s) 306
Cfr13I GGNCC 4 cut(s) 369, 779, 923, 2117
CseI GACGC 1 cut(s) 1602
Csp6I GTAC 9 cut(s) 14, 276, 830, 1236, 1290, 1745, 1780, 2612, 2890
CspAI ACCGGT 1 cut(s) 306
CspCI CAANNNNNGTGG 4 cut(s) 2330, 2365, 3070, 3105
CviQI GTAC 9 cut(s) 14, 276, 830, 1236, 1290, 1745, 1780, 2612, 2890
DpnI GATC 7 cut(s) 78, 92, 468, 591, 1808, 2766, 3233
DpnII GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
DraI TTTAAA 3 cut(s) 627, 699, 3024
Eam1104I CTCTTC 5 cut(s) 51, 1416, 2350, 2364, 2926
EarI CTCTTC 5 cut(s) 51, 1416, 2350, 2364, 2926
Ecl136II GAGCTC 2 cut(s) 1183, 1700
Eco130I CCWWGG 8 cut(s) 901, 1090, 1192, 1558, 1627, 1932, 2095, 2158
Eco147I AGGCCT 1 cut(s) 1089
Eco24I GRGCYC 2 cut(s) 1185, 1702
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 1041
Eco47I GGWCC 4 cut(s) 369, 779, 923, 2117
Eco47III AGCGCT 1 cut(s) 2052
Eco53kI GAGCTC 2 cut(s) 1183, 1700
Eco57I CTGAAG 4 cut(s) 1235, 2084, 2789, 3237
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 2 cut(s) 1183, 1700
EcoO109I RGGNCCY 2 cut(s) 369, 923
EcoRI GAATTC 1 cut(s) 1798
EcoRII CCWGG 3 cut(s) 540, 1176, 1989
EcoRV GATATC 1 cut(s) 1041
EcoT14I CCWWGG 8 cut(s) 901, 1090, 1192, 1558, 1627, 1932, 2095, 2158
EcoT22I ATGCAT 1 cut(s) 1682
EcoT38I GRGCYC 2 cut(s) 1185, 1702
ErhI CCWWGG 8 cut(s) 901, 1090, 1192, 1558, 1627, 1932, 2095, 2158
FalI AAGNNNNNCTT 4 cut(s) 801, 833, 2361, 2393
FaqI GGGAC 2 cut(s) 1776, 3106
FblI GTMKAC 1 cut(s) 2135
Fnu4HI GCNGC 9 cut(s) 6, 150, 1198, 1256, 1480, 1897, 2175, 2316, 2584
FokI GGATG 4 cut(s) 986, 991, 1322, 2927
FriOI GRGCYC 2 cut(s) 1185, 1702
Fsp4HI GCNGC 9 cut(s) 6, 150, 1198, 1256, 1480, 1897, 2175, 2316, 2584
GlaI GCGC 1 cut(s) 2052
GluI GCNGC 9 cut(s) 6, 150, 1198, 1256, 1480, 1897, 2175, 2316, 2584
GsuI CTGGAG 4 cut(s) 1199, 1284, 1716, 2883
HaeII RGCGCY 1 cut(s) 2054
HaeIII GGCC 3 cut(s) 1089, 1095, 3282
HapII CCGG 5 cut(s) 215, 307, 713, 1881, 2240
HgaI GACGC 1 cut(s) 1602
HhaI GCGC 1 cut(s) 2053
Hin6I GCGC 1 cut(s) 2051
HinP1I GCGC 1 cut(s) 2051
HincII GTYRAC 3 cut(s) 108, 728, 1734
HindII GTYRAC 3 cut(s) 108, 728, 1734
HpaI GTTAAC 1 cut(s) 1734
HpaII CCGG 5 cut(s) 215, 307, 713, 1881, 2240
HphI GGTGA 4 cut(s) 61, 88, 1460, 3168
Hpy166II GTNNAC 7 cut(s) 108, 728, 782, 1026, 1734, 1907, 2136
Hpy8I GTNNAC 7 cut(s) 108, 728, 782, 1026, 1734, 1907, 2136
Hpy99I CGWCG 1 cut(s) 779
HpyAV CCTTC 9 cut(s) 523, 827, 1082, 1106, 1394, 1683, 1817, 2495, 3251
HpyCH4III ACNGT 9 cut(s) 266, 548, 779, 842, 1285, 1659, 1744, 2212, 2454
HpyCH4IV ACGT 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 59, 1476, 2550, 2813, 3279
HpySE526I ACGT 1 cut(s) 473
HspAI GCGC 1 cut(s) 2051
Kpn2I TCCGGA 2 cut(s) 214, 712
KpnI GGTACC 1 cut(s) 279
KspAI GTTAAC 1 cut(s) 1734
Kzo9I GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
LguI GCTCTTC 2 cut(s) 51, 2364
LmnI GCTCC 7 cut(s) 217, 1142, 1180, 1697, 1961, 2634, 2902
Lsp1109I GCAGC 9 cut(s) 17, 136, 1209, 1242, 1491, 1908, 2186, 2302, 2570
LweI GCATC 4 cut(s) 1366, 1689, 2986, 3046
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 7 cut(s) 125, 139, 491, 761, 1577, 2266, 2923
MalI GATC 7 cut(s) 78, 92, 468, 591, 1808, 2766, 3233
MboI GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
MfeI CAATTG 5 cut(s) 593, 614, 821, 894, 1978
MhlI GDGCHC 2 cut(s) 1185, 1702
MlyI GAGTC 6 cut(s) 763, 874, 1291, 1730, 2085, 2157
MmeI TCCRAC 2 cut(s) 2132, 2178
Mph1103I ATGCAT 1 cut(s) 1682
MroI TCCGGA 2 cut(s) 214, 712
MroXI GAANNNNTTC 5 cut(s) 229, 816, 986, 2359, 2373
MslI CAYNNNNRTG 1 cut(s) 2739
MspA1I CMGCKG 1 cut(s) 1255
MspI CCGG 5 cut(s) 215, 307, 713, 1881, 2240
MspR9I CCNGG 4 cut(s) 542, 1178, 1991, 2241
MunI CAATTG 5 cut(s) 593, 614, 821, 894, 1978
Mva1269I GAATGC 4 cut(s) 625, 809, 2507, 2534
MvaI CCWGG 3 cut(s) 542, 1178, 1991
MwoI GCNNNNNNNGC 6 cut(s) 50, 59, 1476, 2550, 2813, 3279
NciI CCSGG 1 cut(s) 2241
NcoI CCATGG 1 cut(s) 2158
NdeII GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
NheI GCTAGC 1 cut(s) 296
NlaIV GGNNCC 9 cut(s) 205, 277, 349, 370, 421, 1527, 1957, 2636, 2898
NmuCI GTSAC 2 cut(s) 2266, 2923
NsiI ATGCAT 1 cut(s) 1682
NspI RCATGY 5 cut(s) 14, 661, 1600, 2626, 2813
PaeI GCATGC 1 cut(s) 1600
PagI TCATGA 1 cut(s) 3106
PceI AGGCCT 1 cut(s) 1089
PciI ACATGT 3 cut(s) 10, 657, 2809
PciSI GCTCTTC 2 cut(s) 51, 2364
PctI GAATGC 4 cut(s) 625, 809, 2507, 2534
PdmI GAANNNNTTC 5 cut(s) 229, 816, 986, 2359, 2373
PfeI GAWTC 9 cut(s) 1054, 1366, 1507, 1545, 1994, 2032, 2394, 2852, 3173
PflMI CCANNNNNTGG 2 cut(s) 2114, 2906
PinAI ACCGGT 1 cut(s) 306
PkrI GCNGC 9 cut(s) 7, 151, 1199, 1257, 1481, 1898, 2176, 2317, 2585
PleI GAGTC 6 cut(s) 762, 874, 1291, 1729, 2085, 2157
PpsI GAGTC 6 cut(s) 762, 874, 1291, 1729, 2085, 2157
PpuMI RGGWCCY 2 cut(s) 369, 923
PscI ACATGT 3 cut(s) 10, 657, 2809
Psp124BI GAGCTC 2 cut(s) 1185, 1702
Psp5II RGGWCCY 2 cut(s) 369, 923
Psp6I CCWGG 3 cut(s) 540, 1176, 1989
PspGI CCWGG 3 cut(s) 540, 1176, 1989
PspN4I GGNNCC 9 cut(s) 205, 277, 349, 370, 421, 1527, 1957, 2636, 2898
PspPI GGNCC 4 cut(s) 369, 779, 923, 2117
PspPPI RGGWCCY 2 cut(s) 369, 923
PstI CTGCAG 1 cut(s) 2588
PstNI CAGNNNCTG 1 cut(s) 2583
PvuII CAGCTG 1 cut(s) 1255
RsaI GTAC 9 cut(s) 15, 277, 831, 1237, 1291, 1746, 1781, 2613, 2891
RsaNI GTAC 9 cut(s) 14, 276, 830, 1236, 1290, 1745, 1780, 2612, 2890
RseI CAYNNNNRTG 1 cut(s) 2739
SacI GAGCTC 2 cut(s) 1185, 1702
SapI GCTCTTC 2 cut(s) 51, 2364
SatI GCNGC 9 cut(s) 6, 150, 1198, 1256, 1480, 1897, 2175, 2316, 2584
Sau3AI GATC 7 cut(s) 76, 90, 466, 589, 1806, 2764, 3231
Sau96I GGNCC 4 cut(s) 369, 779, 923, 2117
ScaI AGTACT 1 cut(s) 1291
SchI GAGTC 6 cut(s) 763, 874, 1291, 1730, 2085, 2157
ScrFI CCNGG 4 cut(s) 542, 1178, 1991, 2241
SduI GDGCHC 2 cut(s) 1185, 1702
SfaNI GCATC 4 cut(s) 1366, 1689, 2986, 3046
SfcI CTRYAG 2 cut(s) 1740, 2584
SinI GGWCC 4 cut(s) 369, 779, 923, 2117
SmiI ATTTAAAT 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 2739
SmlI CTYRAG 3 cut(s) 1342, 1474, 2512
SmoI CTYRAG 3 cut(s) 1342, 1474, 2512
SphI GCATGC 1 cut(s) 1600
SseBI AGGCCT 1 cut(s) 1089
SsiI CCGC 4 cut(s) 170, 582, 1140, 2253
SspI AATATT 2 cut(s) 603, 2548
SstI GAGCTC 2 cut(s) 1185, 1702
StuI AGGCCT 1 cut(s) 1089
StyD4I CCNGG 4 cut(s) 540, 1176, 1989, 2239
StyI CCWWGG 8 cut(s) 901, 1090, 1192, 1558, 1627, 1932, 2095, 2158
SwaI ATTTAAAT 1 cut(s) 699
TaaI ACNGT 9 cut(s) 266, 548, 779, 842, 1285, 1659, 1744, 2212, 2454
TaiI ACGT 1 cut(s) 476
TatI WGTACW 4 cut(s) 13, 1289, 1779, 2611
TfiI GAWTC 9 cut(s) 1054, 1366, 1507, 1545, 1994, 2032, 2394, 2852, 3173
TscAI CASTG 8 cut(s) 205, 853, 1041, 1368, 2354, 2425, 2457, 3035
TseFI GTSAC 2 cut(s) 2266, 2923
TseI GCWGC 9 cut(s) 5, 149, 1197, 1255, 1479, 1896, 2174, 2315, 2583
Tsp45I GTSAC 2 cut(s) 2266, 2923
TspDTI ATGAA 6 cut(s) 222, 260, 1620, 1665, 2382, 3032
TspRI CASTG 8 cut(s) 205, 853, 1041, 1368, 2354, 2425, 2457, 3035
Van91I CCANNNNNTGG 2 cut(s) 2114, 2906
VpaK11BI GGWCC 4 cut(s) 369, 779, 923, 2117
XceI RCATGY 5 cut(s) 14, 661, 1600, 2626, 2813
XcmI CCANNNNNNNNNTGG 5 cut(s) 126, 795, 1755, 2165, 2346
XmaJI CCTAGG 2 cut(s) 1192, 1627
XmiI GTMKAC 1 cut(s) 2135
XmnI GAANNNNTTC 5 cut(s) 229, 816, 986, 2359, 2373
ZrmI AGTACT 1 cut(s) 1291
Zsp2I ATGCAT 1 cut(s) 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.