Rroxscaffold_2G00129610

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000058
Physical Location & Seq
Forward (+)
65583177 .. 65587938
4762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129610.1

Sequence Viewer

Length: 3090 bp
ATGGCTGCACAAACCAACCTTAACATCACCACAGACCGGTCTGCTCTTCTTGCTCTCAAAACCCGTATCACCAATGACCCTCAAAACATAGTCTTCTCCAACTGGTCAACCACTACTCTTGTTTGCAACTGGGTTGGAGTCACTTGTGGTGCACGCCATCATCGAATCGCCAAGTTGAACCTCTCTTACTTTGGTCTCATAGGCACCATTCCTCCGGAGCTAGGCAACTTATCTTTTCTTGTTGATCTGGACTTCAGAAATAATAGTTTTCATGGTACCTTACCTCAAGAATTGGCTCGCCTGCGCCGTTTGAAGTTTATTAACTTGGAATACAACAAGTTTATGGGAGTGATTCCATCATGGTTTGGGTCCTTGTCCAAACTTCAAGAATTCAGTTTTTATGGTAATCAATTTTATGGTTCCATCCCAGCTACAATATTCAACTTATCTGCTCTGCAAATAATTGATCTGAGTTATAACCAACTCTCAGGTAGAATACCCAGAGAAATCGGGAACCTAACAATGTTGAAGGAGATCTACCTTGATAATAATAATTTTGAAGGTAGAATGTGTGTTGATTTTTCAGAAATTTCAAATGAGATCGGCACCTTGAATCAACTGGAAGACTTGTGGGTGCAGGAAAATGCCTTAAAAGGGCCAATCCCTAGGTCCGTCTTCAACATGTCATCTTTGACTACTGTAACTTTCTATAGAAACAACTTGAATGGTAGAATTCCCGACAATATATGTCAACATCTTCCAAATATTCAAGGATTGAATTTGGGTATCAATCAGTTTGATGGTCCGCTTCCATCCAAGTTATGGCAATGCAAACAACTTGTTTTATTAAACTTAGAGTTTAACAAGTTCAGTGGAAGAATACCCAAATATATTTGGAACTTAACCCAGATAGAGGAGATTTGGCTTGACACCAACAGCTTGACAGGTACCATACCATATGAGATTGGCTATCTTCCAAATTTAGAGAAGTTGTCGCTCAGTGATAATAATCTAAATGGCCATATCCCATCCTCAATCTTCAATATGTCCACAATAACAGCATTAGATCTCATCTTCAATAAGCTTTCAGGTAGCCTGCCAACAAACATAGGCCTTGGGCTTCCAAACCTAGAAGTGTTTAGAGCAGGAAGGAATCACCTTAGTGGAGTAATCCCCAAATCCATCTCCAATGCTTCTGAGCTTCAGGATCTAGATCTAGGAGATAACTATTTTTCTGGATTTATTCCAAGCACTCTGTGCCTCTTGACAAACCTTGAATGGCTCGCATTGTTCCACAATAATTTGACCATTGATACCTCAACTCCAGAAGTTAATATTTTGTCTTGTCTGCCAAATCTTAGAAATTTGAGGTTTTTAGCCTTTGAAAGCAATCCATTAAATACCAAGCTTTCGTTTTCCTTCAGTAATTTATCTACATCACTTCAACATCTTAGTTTTCTATCTTGCGGCTTGAGAGGTAACATCCCCAGTGATATAGGCAACTTGAGTAGCTTGACAGTTTTAGGCCTTGAAGTAAATCAATTGAGCGGGCCAATTCCAAGCTCAATGGGAAGACTACTGAATCTTCAAGGTTTGGATATGTATGATAACACATTGCAAGGATACATTCCAGATGAACTTTGTAAATTAGAAAACTTAGTTGAATTGGATTTGGGTAGGAATCAACTCTCTGGTTCTATACCTTCCTGCTTGGGTAATCTAACAACATCTCTAAGAACTCTATCCTTACGGTCTAATTTGTTGAATTCCACAATACCATCTACCTTGTGGCGATTTACAGATATCTTGAAACTAGACTTGTCATCCAATTCTCTAAATGGACCTCTATCAGGTGGTGTTGGTAATTTGAAAGTTGCTACATACATAAATATATCATACAACCAATTATCTGGTAGTATACCAAGCAACGTTGGGAATTTACCGAATTTGGTCGATCTCTCCTTAGCACATAATAGTCTAGAAGGTCCTATTCCTAGTTCCTTTGCTACCTGTTTAAGCTTAGTACAGTTGGATTTATCTCAGAACAATCTCTATGGAGTGATTCCGAATTCTCTAGTAGCACTCTCATATCTCAAGTACCTGAATTTGTCTTTCAACAAACTACAGGGAGAAATTCCAACTGGAGGTCCATTCAAAAACTTCTCTGCTAAATCGTTTGTTTCCAACCGTGGACTCTGTGGTCCATCACTATTTGAAGTTCCACCATGCAAAAGAAAAACTAGTATAAAAAAAAATGTGAAAGCTACAGCACATACTATCTTGACGCCTCAAGTTTTATGGAGAAGAGTTTCATACCAAGAGCTCCTACGTGCAACAGATGGATTCAATGAAAGCAACTTACTTGGTACTGGGGGTTTTGGTTCAGTATATAAAGGAACATTGTCAGATGGGATAGACGTTGCAATAAAGGTTTTCAATTTGGAGTTAGAAGGGGCATTTACAAGTTTTGAGGTTGAATGTGAAGTGCTAAGTAATGTTCGTCACCGAAATCTTGTCAAAGTCATCAGTTGCTGCAGTCAAATTGATTTTAAAGGTCTTGTATTGAATTACATGCCTAATGGGAGCCTTGAGAAGTGGTTGTATACTCATAATTCTTCTTTGAACATAATGCAGAGGTTGCATATAATGATAGATGTTGCATCGGCATTGGAGTATCTTCATCATGGTTATGAAGTACCTATTGTCCATTGTGATTTGAAGCCAAGCAACATCCTACTAGATGATGAAATGGTTGCACATGTTGCTGATTTTGGTATTGCGAGACTCTTGGGTGGAGGAGATTCTATGACTCAAACCATGACCCTAGCCACAATTGGGTATATTGCTCCAGATGCGATAGTTGAAGTTGTGGATGCTAGTTTACTTGGAAGTGAGGAGGCTCATGACTTCGTGAGCAAGAGGGAGTGCTTATCATCCATTATGAGATTAGCTGTTGCTTGTTCAGCAGAATCACCAGAAGAGAGGATAAACATGCAAGAGGCTGTAGCCATGCTTAAATCAATCAAGGTCAAGTTTTCAAAGGACACTGATGCAGGAGGTGTAGTGTTGAACCATCATCCTGTTTTGTAA

Protein Analysis

1029

Amino Acids

113.49

Weight (kDa)

5.9

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 11 - 49 2.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 60 - 133 4.8e-07 Leucine-rich repeat region
LRR_8 PF13855 141 - 186 1.9e-06 Leucine rich repeat
LRR_14 PF23598 322 - 515 3.5e-09 Leucine-rich repeat region
LRR_8 PF13855 375 - 435 8.3e-07 Leucine rich repeat
LRR_14 PF23598 440 - 585 5.6e-09 Leucine-rich repeat region
LRR_8 PF13855 504 - 563 3.5e-06 Leucine rich repeat
LRR_8 PF13855 648 - 708 4e-08 Leucine rich repeat
Pkinase PF00069 783 - 953 6.3e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 786 - 955 5.5e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AasI GACNNNNNNGTC 1 cut(s) 2199
Acc65I GGTACC 2 cut(s) 275, 947
AccB1I GGYRCC 4 cut(s) 203, 275, 605, 947
AccB7I CCANNNNNTGG 1 cut(s) 822
AccBSI CCGCTC 1 cut(s) 1548
AccI GTMKAC 2 cut(s) 1918, 2603
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 3 cut(s) 806, 1467, 1548
AclI AACGTT 1 cut(s) 1929
AclWI GGATC 1 cut(s) 1215
AcoI YGGCCR 1 cut(s) 1018
AcuI CTGAAG 3 cut(s) 238, 1187, 1405
AcyI GRCGYC 1 cut(s) 2285
AdeI CACNNNGTG 1 cut(s) 1257
AfaI GTAC 6 cut(s) 277, 949, 2025, 2099, 2368, 2697
AfiI CCNNNNNNNGG 8 cut(s) 36, 221, 654, 822, 913, 1850, 2144, 2835
AflIII ACRYGT 2 cut(s) 681, 2758
AgeI ACCGGT 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 2240
AleI CACNNNNGTG 1 cut(s) 1161
Alw21I GWGCWC 2 cut(s) 154, 2325
Alw26I GTCTC 2 cut(s) 200, 2776
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 1 cut(s) 1215
AlwNI CAGNNNCTG 1 cut(s) 2532
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 5 cut(s) 656, 1018, 1111, 1525, 1550
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 5, 2532
AsiGI ACCGGT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 2308
Asp718I GGTACC 2 cut(s) 275, 947
AspA2I CCTAGG 1 cut(s) 665
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 9 cut(s) 369, 656, 669, 803, 1550, 1841, 1985, 2147, 2201
AsuHPI GGTGA 5 cut(s) 19, 61, 1148, 2495, 2964
AvaII GGWCC 7 cut(s) 369, 669, 803, 1841, 1985, 2147, 2201
AvrII CCTAGG 1 cut(s) 665
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 1020
BanI GGYRCC 4 cut(s) 203, 275, 605, 947
BanII GRGCYC 1 cut(s) 2325
BarI GAAGNNNNNNTAC 2 cut(s) 521, 553
BbsI GAAGAC 4 cut(s) 85, 630, 667, 1579
Bbv12I GWGCWC 2 cut(s) 154, 2325
BbvI GCAGC 1 cut(s) 2519
BceAI ACGGC 1 cut(s) 291
BciVI GTATCC 1 cut(s) 1616
BcoDI GTCTC 2 cut(s) 200, 2776
BcuI ACTAGT 1 cut(s) 2240
BfmI CTRYAG 5 cut(s) 709, 2123, 2265, 2533, 3003
BfuI GTATCC 1 cut(s) 1616
BglII AGATCT 3 cut(s) 534, 1066, 1213
BisI GCNGC 3 cut(s) 6, 1468, 2533
BlnI CCTAGG 1 cut(s) 665
BlsI GCNGC 3 cut(s) 7, 1469, 2534
Bme18I GGWCC 7 cut(s) 369, 669, 803, 1841, 1985, 2147, 2201
BmgT120I GGNCC 9 cut(s) 369, 656, 669, 803, 1550, 1841, 1985, 2147, 2201
BmiI GGNNCC 8 cut(s) 205, 277, 370, 421, 515, 607, 949, 2585
BmrI ACTGGG 3 cut(s) 139, 1482, 2379
BmsI GCATC 4 cut(s) 2669, 2842, 2863, 3040
BmuI ACTGGG 3 cut(s) 139, 1482, 2379
BpiI GAAGAC 4 cut(s) 85, 630, 667, 1579
BpmI CTGGAG 3 cut(s) 1308, 2163, 2832
Bpu10I CCTNAGC 1 cut(s) 1963
BpuEI CTTGAG 6 cut(s) 270, 1492, 1525, 2078, 2274, 2609
BsaAI YACGTR 1 cut(s) 2330
BsaBI GATNNNNATC 2 cut(s) 1008, 1212
BsaHI GRCGYC 1 cut(s) 2285
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 3 cut(s) 665, 1114, 2188
BsaWI WCCGGW 2 cut(s) 36, 214
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 1306, 1336, 3048, 3078
Bsc4I CCNNNNNNNGG 8 cut(s) 36, 221, 654, 822, 913, 1850, 2144, 2835
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 6 cut(s) 107, 134, 624, 1488, 2146, 2374
Bse3DI GCAATG 2 cut(s) 833, 1613
Bse8I GATNNNNATC 2 cut(s) 1008, 1212
BseAI TCCGGA 1 cut(s) 214
BseDI CCNNGG 3 cut(s) 665, 1114, 2188
BseGI GGATG 9 cut(s) 423, 812, 1028, 1482, 1823, 2730, 2878, 2933, 3076
BseJI GATNNNNATC 2 cut(s) 1008, 1212
BseLI CCNNNNNNNGG 8 cut(s) 36, 221, 654, 822, 913, 1850, 2144, 2835
BseMI GCAATG 2 cut(s) 833, 1613
BseMII CTCAG 5 cut(s) 461, 501, 1012, 1188, 2054
BseNI ACTGG 6 cut(s) 107, 134, 624, 1488, 2146, 2374
BseRI GAGGAG 3 cut(s) 929, 2811, 2909
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 1 cut(s) 2519
BseYI CCCAGC 1 cut(s) 427
BsgI GTGCAG 1 cut(s) 656
BshFI GGCC 5 cut(s) 658, 1020, 1113, 1527, 1552
BshNI GGYRCC 4 cut(s) 203, 275, 605, 947
BshTI ACCGGT 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 154, 2325
BsiSI CCGG 2 cut(s) 37, 215
BslI CCNNNNNNNGG 8 cut(s) 36, 221, 654, 822, 913, 1850, 2144, 2835
BsmAI GTCTC 2 cut(s) 200, 2776
BsnI GGCC 5 cut(s) 658, 1020, 1113, 1527, 1552
Bso31I GGTCTC 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 154, 2325
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
BspACI CCGC 3 cut(s) 806, 1467, 1548
BspANI GGCC 5 cut(s) 658, 1020, 1113, 1527, 1552
BspCNI CTCAG 5 cut(s) 462, 500, 1011, 1189, 2053
BspEI TCCGGA 1 cut(s) 214
BspHI TCATGA 1 cut(s) 2902
BspLI GGNNCC 8 cut(s) 205, 277, 370, 421, 515, 607, 949, 2585
BspMAI CTGCAG 1 cut(s) 2537
BspPI GGATC 1 cut(s) 1215
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 4 cut(s) 203, 275, 605, 947
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 1548
BsrDI GCAATG 2 cut(s) 833, 1613
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 6 cut(s) 107, 134, 624, 1488, 2146, 2374
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 3 cut(s) 665, 1114, 2188
BssMI GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
BssNAI GTATAC 2 cut(s) 1919, 2604
BssNI GRCGYC 1 cut(s) 2285
BssT1I CCWWGG 2 cut(s) 665, 1114
Bst1107I GTATAC 2 cut(s) 1919, 2604
Bst4CI ACNGT 5 cut(s) 700, 1519, 1752, 2028, 2189
Bst6I CTCTTC 3 cut(s) 51, 2299, 2973
BstACI GRCGYC 1 cut(s) 2285
BstAPI GCANNNNNTGC 3 cut(s) 1257, 2638, 2762
BstBAI YACGTR 1 cut(s) 2330
BstC8I GCNNGC 6 cut(s) 154, 298, 302, 1097, 1284, 1550
BstDSI CCRYGG 1 cut(s) 2188
BstENI CCTNNNNNAGG 1 cut(s) 1848
BstF5I GGATG 9 cut(s) 423, 812, 1028, 1482, 1823, 2730, 2878, 2933, 3076
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 8 cut(s) 247, 469, 537, 603, 1069, 1210, 1216, 1957
BstMAI GTCTC 2 cut(s) 200, 2776
BstMBI GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
BstMWI GCNNNNNNNGC 6 cut(s) 50, 1257, 2638, 2762, 2852, 2963
BstNSI RCATGY 4 cut(s) 685, 2575, 2762, 2995
BstSFI CTRYAG 5 cut(s) 709, 2123, 2265, 2533, 3003
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 2519
BstV2I GAAGAC 4 cut(s) 85, 630, 667, 1579
BstX2I RGATCY 4 cut(s) 534, 1066, 1207, 1213
BstXI CCANNNNNNTGG 1 cut(s) 1910
BstYI RGATCY 4 cut(s) 534, 1066, 1207, 1213
BstZ17I GTATAC 2 cut(s) 1919, 2604
BsuI GTATCC 1 cut(s) 1616
BsuRI GGCC 5 cut(s) 658, 1020, 1113, 1527, 1552
BtgI CCRYGG 1 cut(s) 2188
BtsCI GGATG 9 cut(s) 423, 812, 1028, 1482, 1823, 2730, 2878, 2933, 3076
BtsIMutI CAGTG 4 cut(s) 877, 1006, 1495, 3045
Cac8I GCNNGC 6 cut(s) 154, 298, 302, 1097, 1284, 1550
CaiI CAGNNNCTG 1 cut(s) 2532
CciI TCATGA 1 cut(s) 2902
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 9 cut(s) 369, 656, 669, 803, 1550, 1841, 1985, 2147, 2201
CseI GACGC 1 cut(s) 2293
Csp6I GTAC 6 cut(s) 276, 948, 2024, 2098, 2367, 2696
CspAI ACCGGT 1 cut(s) 36
CspCI CAANNNNNGTGG 4 cut(s) 100, 135, 853, 888
CviQI GTAC 6 cut(s) 276, 948, 2024, 2098, 2367, 2696
DpnI GATC 8 cut(s) 246, 468, 536, 602, 1068, 1209, 1215, 1956
DpnII GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
DraI TTTAAA 1 cut(s) 2551
DraIII CACNNNGTG 1 cut(s) 1257
DrdI GACNNNNNNGTC 1 cut(s) 2199
DseDI GACNNNNNNGTC 1 cut(s) 2199
EaeI YGGCCR 1 cut(s) 1018
Eam1104I CTCTTC 3 cut(s) 51, 2299, 2973
EarI CTCTTC 3 cut(s) 51, 2299, 2973
Ecl136II GAGCTC 1 cut(s) 2323
Eco130I CCWWGG 2 cut(s) 665, 1114
Eco147I AGGCCT 2 cut(s) 1113, 1527
Eco24I GRGCYC 1 cut(s) 2325
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 1804
Eco47I GGWCC 7 cut(s) 369, 669, 803, 1841, 1985, 2147, 2201
Eco53kI GAGCTC 1 cut(s) 2323
Eco57I CTGAAG 3 cut(s) 238, 1187, 1405
EcoICRI GAGCTC 1 cut(s) 2323
EcoNI CCTNNNNNAGG 1 cut(s) 1848
EcoO109I RGGNCCY 2 cut(s) 369, 1985
EcoRI GAATTC 4 cut(s) 389, 732, 1765, 2068
EcoRV GATATC 1 cut(s) 1804
EcoT14I CCWWGG 2 cut(s) 665, 1114
EcoT38I GRGCYC 1 cut(s) 2325
ErhI CCWWGG 2 cut(s) 665, 1114
FauI CCCGC 1 cut(s) 1541
FauNDI CATATG 1 cut(s) 958
FblI GTMKAC 2 cut(s) 1918, 2603
Fnu4HI GCNGC 3 cut(s) 6, 1468, 2533
FokI GGATG 9 cut(s) 410, 799, 1015, 1469, 1810, 2717, 2885, 2920, 3063
FriOI GRGCYC 1 cut(s) 2325
Fsp4HI GCNGC 3 cut(s) 6, 1468, 2533
GlaI GCGC 1 cut(s) 305
GluI GCNGC 3 cut(s) 6, 1468, 2533
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 3 cut(s) 1308, 2163, 2832
HaeIII GGCC 5 cut(s) 658, 1020, 1113, 1527, 1552
HapII CCGG 2 cut(s) 37, 215
HgaI GACGC 1 cut(s) 2293
HhaI GCGC 1 cut(s) 306
Hin1I GRCGYC 1 cut(s) 2285
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 2 cut(s) 108, 752
HindII GTYRAC 2 cut(s) 108, 752
HindIII AAGCTT 3 cut(s) 1082, 1406, 2017
HpaII CCGG 2 cut(s) 37, 215
HphI GGTGA 5 cut(s) 19, 61, 1148, 2495, 2964
Hpy166II GTNNAC 8 cut(s) 108, 152, 752, 1050, 1919, 2192, 2604, 2882
Hpy188I TCNGA 7 cut(s) 257, 471, 586, 1198, 2043, 2067, 2407
Hpy8I GTNNAC 8 cut(s) 108, 152, 752, 1050, 1919, 2192, 2604, 2882
HpyAV CCTTC 7 cut(s) 523, 554, 1143, 1429, 1713, 1976, 2444
HpyCH4III ACNGT 5 cut(s) 700, 1519, 1752, 2028, 2189
HpyCH4IV ACGT 3 cut(s) 1929, 2329, 2418
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 1257, 2638, 2762, 2852, 2963
HpySE526I ACGT 3 cut(s) 1929, 2329, 2418
Hsp92I GRCGYC 1 cut(s) 2285
HspAI GCGC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 214
KpnI GGTACC 2 cut(s) 279, 951
Kzo9I GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 4 cut(s) 217, 2328, 2583, 2851
Lsp1109I GCAGC 1 cut(s) 2519
LweI GCATC 4 cut(s) 2669, 2842, 2863, 3040
MaeII ACGT 3 cut(s) 1929, 2329, 2418
MaeIII GTNAC 4 cut(s) 139, 700, 1478, 2501
MalI GATC 8 cut(s) 246, 468, 536, 602, 1068, 1209, 1215, 1956
MbiI CCGCTC 1 cut(s) 1548
MboI GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
MfeI CAATTG 2 cut(s) 1541, 2832
MflI RGATCY 4 cut(s) 534, 1066, 1207, 1213
MhlI GDGCHC 2 cut(s) 154, 2325
MlsI TGGCCA 1 cut(s) 1020
MluNI TGGCCA 1 cut(s) 1020
MlyI GAGTC 4 cut(s) 147, 2187, 2778, 2803
MmeI TCCRAC 5 cut(s) 115, 123, 2010, 2162, 2208
Mox20I TGGCCA 1 cut(s) 1020
MroI TCCGGA 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 2308
MscI TGGCCA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 1161, 2688
Msp20I TGGCCA 1 cut(s) 1020
MspI CCGG 2 cut(s) 37, 215
MunI CAATTG 2 cut(s) 1541, 2832
MwoI GCNNNNNNNGC 6 cut(s) 50, 1257, 2638, 2762, 2852, 2963
NdeI CATATG 1 cut(s) 958
NdeII GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
NlaIV GGNNCC 8 cut(s) 205, 277, 370, 421, 515, 607, 949, 2585
NmuCI GTSAC 2 cut(s) 139, 2501
NspI RCATGY 4 cut(s) 685, 2575, 2762, 2995
OliI CACNNNNGTG 1 cut(s) 1161
PagI TCATGA 1 cut(s) 2902
PceI AGGCCT 2 cut(s) 1113, 1527
PciI ACATGT 2 cut(s) 681, 2758
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 2 cut(s) 160, 304
PdmI GAANNNNTTC 1 cut(s) 2308
PflMI CCANNNNNTGG 1 cut(s) 822
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 3 cut(s) 7, 1469, 2534
PleI GAGTC 4 cut(s) 146, 2187, 2778, 2803
PpsI GAGTC 4 cut(s) 146, 2187, 2778, 2803
Ppu21I YACGTR 1 cut(s) 2330
PpuMI RGGWCCY 2 cut(s) 369, 1985
PscI ACATGT 2 cut(s) 681, 2758
PsiI TTATAA 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 2325
Psp1406I AACGTT 1 cut(s) 1929
Psp5II RGGWCCY 2 cut(s) 369, 1985
PspFI CCCAGC 1 cut(s) 427
PspN4I GGNNCC 8 cut(s) 205, 277, 370, 421, 515, 607, 949, 2585
PspPI GGNCC 9 cut(s) 369, 656, 669, 803, 1550, 1841, 1985, 2147, 2201
PspPPI RGGWCCY 2 cut(s) 369, 1985
PsrI GAACNNNNNNTAC 2 cut(s) 170, 202
PstI CTGCAG 1 cut(s) 2537
PstNI CAGNNNCTG 1 cut(s) 2532
PsuI RGATCY 4 cut(s) 534, 1066, 1207, 1213
RsaI GTAC 6 cut(s) 277, 949, 2025, 2099, 2368, 2697
RsaNI GTAC 6 cut(s) 276, 948, 2024, 2098, 2367, 2696
RseI CAYNNNNRTG 2 cut(s) 1161, 2688
SacI GAGCTC 1 cut(s) 2325
SapI GCTCTTC 1 cut(s) 51
SatI GCNGC 3 cut(s) 6, 1468, 2533
Sau3AI GATC 8 cut(s) 244, 466, 534, 600, 1066, 1207, 1213, 1954
Sau96I GGNCC 9 cut(s) 369, 656, 669, 803, 1550, 1841, 1985, 2147, 2201
SchI GAGTC 4 cut(s) 147, 2187, 2778, 2803
SduI GDGCHC 2 cut(s) 154, 2325
SfaNI GCATC 4 cut(s) 2669, 2842, 2863, 3040
SfcI CTRYAG 5 cut(s) 709, 2123, 2265, 2533, 3003
SinI GGWCC 7 cut(s) 369, 669, 803, 1841, 1985, 2147, 2201
SmiMI CAYNNNNRTG 2 cut(s) 1161, 2688
SmlI CTYRAG 6 cut(s) 285, 1471, 1504, 2093, 2289, 2588
SmoI CTYRAG 6 cut(s) 285, 1471, 1504, 2093, 2289, 2588
SpeI ACTAGT 1 cut(s) 2240
SseBI AGGCCT 2 cut(s) 1113, 1527
SsiI CCGC 3 cut(s) 806, 1467, 1548
SspI AATATT 3 cut(s) 438, 766, 1336
SstI GAGCTC 1 cut(s) 2325
StuI AGGCCT 2 cut(s) 1113, 1527
StyI CCWWGG 2 cut(s) 665, 1114
TaaI ACNGT 5 cut(s) 700, 1519, 1752, 2028, 2189
TaiI ACGT 3 cut(s) 1932, 2332, 2421
TaqI TCGA 2 cut(s) 163, 1953
TatI WGTACW 1 cut(s) 2023
TauI GCSGC 1 cut(s) 1470
TscAI CASTG 4 cut(s) 877, 1006, 1495, 3052
TseFI GTSAC 2 cut(s) 139, 2501
TseI GCWGC 2 cut(s) 5, 2532
Tsp45I GTSAC 2 cut(s) 139, 2501
TspDTI ATGAA 7 cut(s) 260, 1650, 2301, 2364, 2669, 2706, 2760
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 4 cut(s) 877, 1006, 1495, 3052
Van91I CCANNNNNTGG 1 cut(s) 822
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 7 cut(s) 369, 669, 803, 1841, 1985, 2147, 2201
XagI CCTNNNNNAGG 1 cut(s) 1848
XbaI TCTAGA 2 cut(s) 1210, 1978
XceI RCATGY 4 cut(s) 685, 2575, 2762, 2995
XcmI CCANNNNNNNNNTGG 2 cut(s) 819, 1785
XmaJI CCTAGG 1 cut(s) 665
XmiI GTMKAC 2 cut(s) 1918, 2603
XmnI GAANNNNTTC 1 cut(s) 2308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.