RchiOBHm_Chr4g0403281

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000058
Physical Location & Seq
Forward (+)
22359810 .. 22363629
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37504

Sequence Viewer

Length: 3333 bp
ATGGAAAACCCAAAAACTCTCTTTCTTTTTGGTGTTCTCTTACTGCAGTACTCATGCTTACTTGAGTTGGCCATATCTTCTTCCAACTTCACCGATCAATCTGCTCTTCTTGCAATCCAATCCAAGCTCACTTTCGACCCCTCCAATACTGTATTGGGCGGTAATTGGACCACCAACACAAGCTTCTGTAATTGGATTGGAGTCTCTTGTAGTATTCACAGGCAAAGAGTCACAGCCCTCAATCTGTCCTTCATGGGTCTCCAAGGCACCATTGCACCTCATGTTGGCAACCTCTCCTTCCTAGTCTTGCTCGATCTTCGAAACAACAGCTTCTCTGGGGCACTGCCACATGAGATCAGTTGCTTGCATCGCTTGAGGATACTTAAATCTAGTTACAATCAGTTGGAAGGTAACATCCCTCCAAGTTTATATTATTGTCAAAAGCTTGAAATCATATACCTCCAAAATAACAGGCTAAGCAGTTTTATACCTCTAGAGTTGGGATTCCTACCTAGCCTTCAAGAATTATATCTCCATGAAAATAAACTTACGGGTGGAATACCGTCATCTTTGGGCAATATTTCAACATTAAAAGTGTTGAGTTTGGGTGTAAATAAACTCACCGGTTCATTACCTTCTACTCTCCTCAACCTGTCTTCTCTAGTTAAAGTCTATATATATAGTAACAACATATCTGGAAGCCTACCTATGGATCTTTGCCACTATTGGCCTAGTATTCAGATCCTTTCTTTTGGGGGCCTTAACCTTGTTGGCCAAATTCCCTCAAGAATATATGAATGCACACAGCTTGTGGCCTTATCTGTGCCTTATAATATGCTTGTGGGAAATATTCCAGAAACAATTGGGCTTTTACAAAATCTTGAAGAGCTTTATCTGGGTGCTAATGGCCTAACTGGCACCATACCTCCTACCATTGGTAACATGTCGAGCTTGAAAGTCTTTGATTTGGGGCCTAATTACATTACAGGAAGTATTCCGAGGGAACTGGGGCGTCTTTCCAATCTAAACTTGTTTACTTTTTATGGAAATAGCCTAACAGATGCAATACCTCAAGAACTTTTCAACATGTCATCCTTGCAAACTTTCTCACTTCAACATAATTCCCTTTCTGGAACTTTTCCTCCATACAACAAGATTTTTCTTCCCAACCTTGAAAACCTCTATTATTCTAGTAATCAAATAACTGGAAACATCCCATCATATTTTACCAATTTTAGCAACCTTTATGATCTTGATGCTGCCAACAACTTACTCTATGGACCAATACCCACGAACCTTGGTAGCTTGAAACACCTGGAACTCCTTATATTGGGAGGAAACCATTTAACAGGAGAACCTGGAGTTAAAGAACTTAGATTCCTTTCCTCTTTATTTAATTCCACTTCTCTGACCCTTTTGGCTTTGGAAGACAATCCTCTAAATGGTGTCATACCCGATATCTTGGGAAATTACTCTTATTCCATCAGGCAAATATATGCACCAGGGTGCAAAATCCAGGGTCATATACCTAAGACAATTGGCTCTTTGAAAAATTTGACCACCATCGTCTTCATGAATAACAATATCAGTGGTAATTTACCATCCTCAATTGGGAAATTGGAGATGATGCAGAGATTGTATCTTGATAACAACAATATTGAAGAAGCCATTTCAGAGGAGCTTTGTCTTATGGTGAACCTGGGAGAACTGTCTTTATCAAATAACAAATTCTCAGGGTCTATCCCAAATTGTATAGGAAACCTCAGCCTCCTTCAAAGAGTTTACCTAGATTCCAATAGGCTGACATCGTCAGTCCCAGTGAATTTATGGAACCTGGAAGGCCTCTTGTTCTTGAATTTGTCATCCAATTCCATGGCCGGAGATTTACCTACAAGAATGGGAAAGCTTAATGTTCTTCAAATGGTTGATTTATCTTGGAACCAAATTAGGGGAAGTATTCCAAGCATCATTGGAACTTTTCAAAGCTTGAACTGGCTAGATTTGTCAAACAACATGTTTGTAGGACACATTCCACAAAGTTTCAGTGATCTGAAAGGGTTGGAATATTTGGATCTGTCATACAATAGATTGTCGGGCACAATACCAAAGTCTCTTGAAAAACTCAGGTATCTCAATTATCTGAATTTCTCATTTAATAAACTCTCTGGTGAGATTCCTTCTGGGGGACCGTTTGTCAACTTCACTGATCAATCATTTTTGGAGAATGGAGTACTTTGCGGCAGATCAAATTTTGGAGTCCCACCATGTAAGGCTCATAGCAAAAGGAAATCAAATATGGCTTTTCATTGGCTCAAATATATTCTTCCAGCAATTGCATCCACAACAATTCTCCTGGGTCTTATTCTACTGTGGAAAAAGTATCATAAACGGAAAGCATCATTGCCAAGTTCCGATGAAAAGTCACCATTAACAGAGCACAAAATGGTATCATATCAAGAACTCCGCCTTGCTACAAATGACTTCTGCGGAAGCAACTTAATTGGAGTGGGAAGTTTTGGCTCTGTGTACAAGGGATTGCTCTCTGATGGGACAATTGTTGCTATAAAGGTTCTAAATTTGCAAATGGAAGGGGCCTCCAAAAGTTTTGATGCAGAATGCAAGGTGTTGCGAACAATCCGGCATAGGAATCTTGTAAAGGTCATAACTACATGTTCTAGCCCTGAGGTCAGAGCTTTGGTGCTGCAGTACATGTCTAATGGCAGTCTTGAGAAGTGGTTATACTCTCACAACTACTGTCTCAGTATTCTGCAAAGAGTAAGCATGTTGATCGATATTGCATCTGCTTTGGAATATCTCCATCATGGTCAAGAAGAAACTGTGGTGCATTGTGATGTGAAGCCTGGCAATGTCCTTCTTGATGAAGACATGGTTGCACATGTTGCAGACTTCGGTCTTGCAAAAATTTTAGCAAAAAACAAAGACGCAACACAGACAAGAACCATCGGTACTGTGGGTTATGTAGCACCAGAGTATGGTTCTACAGGAAGAGTGTCCAGAAGTGGCGATGTTTATAGCTTCGGGATAATGTTGCTAGAAATAATGGCGAGAAAGAAACCAACTGATGAAATGTTTGCTGGAGAAGTGACTTTGAGGCAATGGGTAAATGTGTCTATCCCTGACAATGCTCTAGAAGTGGTGGATGCTGACTTATTGAGCATAGAAGAGGGAAGAGACATGTATGCCACAGAATCTATCCTTATGTCCATCCTGGAAATAGGCTTGAGGTGTTCCGAAGTAGCAGCAGAGGATAGAATGGACATTAAGGATGTGGTGCCCAAGCTCAAGAAAATAAAACTGGCACTACAAGAATGA

Protein Analysis

1110

Amino Acids

122.64

Weight (kDa)

6.42

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 71 6.7e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 85 - 274 2.8e-10 Leucine-rich repeat region
LRR_14 PF23598 257 - 448 1.7e-09 Leucine-rich repeat region
LRR_14 PF23598 508 - 593 2.3e-06 Leucine-rich repeat region
LRR_14 PF23598 608 - 721 1.8e-10 Leucine-rich repeat region
LRR_8 PF13855 662 - 721 1.3e-09 Leucine rich repeat
Pkinase PF00069 832 - 1028 8.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 833 - 1100 4.3e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 831
AccB1I GGYRCC 3 cut(s) 266, 917, 3292
AccB7I CCANNNNNTGG 1 cut(s) 2993
AciI CCGC 4 cut(s) 159, 2238, 2464, 2487
AclWI GGATC 3 cut(s) 720, 736, 2079
AcoI YGGCCR 3 cut(s) 69, 772, 1875
AcsI RAATTY 9 cut(s) 777, 1552, 1727, 1822, 1855, 2143, 2248, 2575, 2922
AcyI GRCGYC 1 cut(s) 1012
AfaI GTAC 5 cut(s) 50, 2232, 2528, 2708, 2968
AfiI CCNNNNNNNGG 9 cut(s) 284, 709, 935, 1330, 1442, 1611, 1948, 2183, 2993
AflIII ACRYGT 7 cut(s) 942, 1086, 2013, 2669, 2709, 2896, 3195
AgeI ACCGGT 1 cut(s) 623
AhdI GACNNNNNGTC 1 cut(s) 2192
AjnI CCWGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 167, 199
AleI CACNNNNGTG 1 cut(s) 1504
AloI GAACNNNNNNTCC 4 cut(s) 1126, 1158, 1362, 1394
Alw21I GWGCWC 1 cut(s) 2439
Alw26I GTCTC 5 cut(s) 208, 263, 2115, 2762, 3186
AlwI GGATC 3 cut(s) 720, 736, 2079
ApeKI GCWGC 3 cut(s) 1259, 2701, 3260
ApoI RAATTY 9 cut(s) 777, 1552, 1727, 1822, 1855, 2143, 2248, 2575, 2922
AsiGI ACCGGT 1 cut(s) 623
AspS9I GGNCC 6 cut(s) 168, 757, 971, 1280, 2186, 2592
AsuHPI GGTGA 5 cut(s) 82, 613, 1705, 2180, 2415
AsuII TTCGAA 1 cut(s) 319
AvaII GGWCC 3 cut(s) 168, 1280, 2186
AxyI CCTNAGG 1 cut(s) 2682
BaeGI GKGCMC 3 cut(s) 343, 2099, 3297
BaeI ACNNNNGTAYC 2 cut(s) 2111, 2144
BalI TGGCCA 2 cut(s) 71, 774
BanI GGYRCC 3 cut(s) 266, 917, 3292
BarI GAAGNNNNNNTAC 2 cut(s) 2723, 2755
BbsI GAAGAC 4 cut(s) 648, 1434, 1561, 2889
Bbv12I GWGCWC 1 cut(s) 2439
BbvCI CCTCAGC 1 cut(s) 1763
BbvI GCAGC 3 cut(s) 1246, 2688, 3272
BccI CCATC 8 cut(s) 1225, 1490, 1571, 1609, 2540, 2826, 2969, 3233
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 2770, 2804
BciT130I CCWGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
BciVI GTATCC 1 cut(s) 372
BclI TGATCA 1 cut(s) 2206
BcoDI GTCTC 5 cut(s) 208, 263, 2115, 2762, 3186
BfmI CTRYAG 3 cut(s) 44, 2702, 3000
BfuI GTATCC 1 cut(s) 372
BglI GCCNNNNNGGC 1 cut(s) 915
BisI GCNGC 4 cut(s) 1260, 2239, 2702, 3261
BlpI GCTNAGC 1 cut(s) 476
BlsI GCNGC 4 cut(s) 1261, 2240, 2703, 3262
BmcAI AGTACT 2 cut(s) 50, 2232
Bme1390I CCNGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
Bme18I GGWCC 3 cut(s) 168, 1280, 2186
BmeRI GACNNNNNGTC 1 cut(s) 2192
BmgT120I GGNCC 6 cut(s) 168, 757, 971, 1280, 2186, 2592
BmiI GGNNCC 9 cut(s) 268, 758, 919, 972, 1832, 1940, 2187, 2593, 3294
BmrFI CCNGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
BmrI ACTGGG 2 cut(s) 1016, 1811
BmuI ACTGGG 2 cut(s) 1016, 1811
BoxI GACNNNNGTC 1 cut(s) 2910
BpiI GAAGAC 4 cut(s) 648, 1434, 1561, 2889
BpmI CTGGAG 2 cut(s) 1380, 3117
Bpu10I CCTNAGC 1 cut(s) 1763
Bpu1102I GCTNAGC 1 cut(s) 476
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 7 cut(s) 83, 394, 769, 1056, 2747, 3262, 3287
Bsa29I ATCGAT 1 cut(s) 2790
BsaHI GRCGYC 1 cut(s) 1012
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 8 cut(s) 262, 998, 1297, 1502, 1516, 1699, 1872, 2353
BsaWI WCCGGW 1 cut(s) 623
BsaXI ACNNNNNCTCC 4 cut(s) 910, 940, 1126, 1156
Bsc4I CCNNNNNNNGG 9 cut(s) 284, 709, 935, 1330, 1442, 1611, 1948, 2183, 2993
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 6 cut(s) 919, 1011, 1210, 1817, 1997, 3321
Bse21I CCTNAGG 1 cut(s) 2682
Bse3DI GCAATG 4 cut(s) 270, 2399, 2872, 3122
BseBI CCWGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
BseCI ATCGAT 1 cut(s) 2790
BseDI CCNNGG 8 cut(s) 262, 998, 1297, 1502, 1516, 1699, 1872, 2353
BseGI GGATG 9 cut(s) 414, 1091, 1212, 1601, 1862, 2336, 3166, 3225, 3292
BseLI CCNNNNNNNGG 9 cut(s) 284, 709, 935, 1330, 1442, 1611, 1948, 2183, 2993
BseMI GCAATG 4 cut(s) 270, 2399, 2872, 3122
BseMII CTCAG 5 cut(s) 1746, 1777, 2137, 2673, 2773
BseNI ACTGG 6 cut(s) 919, 1011, 1210, 1817, 1997, 3321
BseRI GAGGAG 2 cut(s) 635, 1691
BseSI GKGCMC 3 cut(s) 343, 2099, 3297
BseXI GCAGC 3 cut(s) 1246, 2688, 3272
BshNI GGYRCC 3 cut(s) 266, 917, 3292
BshTI ACCGGT 1 cut(s) 623
BshVI ATCGAT 1 cut(s) 2790
BsiHKAI GWGCWC 1 cut(s) 2439
BsiSI CCGG 3 cut(s) 624, 1878, 2638
BslFI GGGAC 4 cut(s) 1799, 2199, 2243, 2563
BslI CCNNNNNNNGG 9 cut(s) 284, 709, 935, 1330, 1442, 1611, 1948, 2183, 2993
BsmAI GTCTC 5 cut(s) 208, 263, 2115, 2762, 3186
BsmFI GGGAC 4 cut(s) 1799, 2199, 2243, 2563
BsmI GAATGC 2 cut(s) 803, 2621
Bso31I GGTCTC 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 319
Bsp1286I GDGCHC 4 cut(s) 343, 2099, 2439, 3297
Bsp1407I TGTACA 1 cut(s) 2526
Bsp1720I GCTNAGC 1 cut(s) 476
Bsp19I CCATGG 1 cut(s) 1872
BspACI CCGC 4 cut(s) 159, 2238, 2464, 2487
BspCNI CTCAG 5 cut(s) 1745, 1776, 2136, 2674, 2772
BspDI ATCGAT 1 cut(s) 2790
BspHI TCATGA 1 cut(s) 1572
BspLI GGNNCC 9 cut(s) 268, 758, 919, 972, 1832, 1940, 2187, 2593, 3294
BspMAI CTGCAG 2 cut(s) 48, 2706
BspPI GGATC 3 cut(s) 720, 736, 2079
BspQI GCTCTTC 2 cut(s) 111, 879
BspT104I TTCGAA 1 cut(s) 319
BspT107I GGYRCC 3 cut(s) 266, 917, 3292
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 4 cut(s) 270, 2399, 2872, 3122
BsrFI RCCGGY 1 cut(s) 623
BsrGI TGTACA 1 cut(s) 2526
BsrI ACTGG 6 cut(s) 919, 1011, 1210, 1817, 1997, 3321
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 8 cut(s) 262, 998, 1297, 1502, 1516, 1699, 1872, 2353
BssNI GRCGYC 1 cut(s) 1012
BssT1I CCWWGG 3 cut(s) 262, 1297, 1872
Bst2UI CCWGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
Bst4CI ACNGT 8 cut(s) 151, 564, 1710, 2190, 2370, 2756, 2839, 2971
Bst6I CTCTTC 5 cut(s) 111, 879, 3001, 3177, 3184
BstACI GRCGYC 1 cut(s) 1012
BstAPI GCANNNNNTGC 1 cut(s) 2900
BstAUI TGTACA 1 cut(s) 2526
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 8 cut(s) 476, 1373, 1530, 1732, 1763, 2123, 2682, 2759
BstDSI CCRYGG 1 cut(s) 1872
BstF5I GGATG 9 cut(s) 414, 1091, 1212, 1601, 1862, 2336, 3166, 3225, 3292
BstMAI GTCTC 5 cut(s) 208, 263, 2115, 2762, 3186
BstMWI GCNNNNNNNGC 4 cut(s) 110, 369, 915, 2900
BstNI CCWGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
BstNSI RCATGY 8 cut(s) 946, 1090, 2017, 2673, 2713, 2785, 2900, 3199
BstPAI GACNNNNGTC 1 cut(s) 2910
BstSCI CCNGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
BstSFI CTRYAG 3 cut(s) 44, 2702, 3000
BstSLI GKGCMC 3 cut(s) 343, 2099, 3297
BstV1I GCAGC 3 cut(s) 1246, 2688, 3272
BstV2I GAAGAC 4 cut(s) 648, 1434, 1561, 2889
BstX2I RGATCY 3 cut(s) 712, 741, 2071
BstXI CCANNNNNNTGG 1 cut(s) 1873
BstYI RGATCY 3 cut(s) 712, 741, 2071
Bsu15I ATCGAT 1 cut(s) 2790
Bsu36I CCTNAGG 1 cut(s) 2682
BsuI GTATCC 1 cut(s) 372
BsuTUI ATCGAT 1 cut(s) 2790
BtgI CCRYGG 1 cut(s) 1872
BtgZI GCGATG 2 cut(s) 353, 3039
BtsCI GGATG 9 cut(s) 414, 1091, 1212, 1601, 1862, 2336, 3166, 3225, 3292
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 5 cut(s) 341, 1594, 1824, 2050, 2202
Cac8I GCNNGC 1 cut(s) 365
CciI TCATGA 1 cut(s) 1572
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 6 cut(s) 168, 757, 971, 1280, 2186, 2592
ClaI ATCGAT 1 cut(s) 2790
CseI GACGC 2 cut(s) 1001, 2951
Csp6I GTAC 5 cut(s) 49, 2231, 2527, 2707, 2967
CspAI ACCGGT 1 cut(s) 623
CspCI CAANNNNNGTGG 4 cut(s) 1279, 1314, 1570, 1605
CviQI GTAC 5 cut(s) 49, 2231, 2527, 2707, 2967
DdeI CTNAG 8 cut(s) 476, 1373, 1530, 1732, 1763, 2123, 2682, 2759
DriI GACNNNNNGTC 1 cut(s) 2192
EaeI YGGCCR 3 cut(s) 69, 772, 1875
Eam1104I CTCTTC 5 cut(s) 111, 879, 3001, 3177, 3184
Eam1105I GACNNNNNGTC 1 cut(s) 2192
EarI CTCTTC 5 cut(s) 111, 879, 3001, 3177, 3184
EciI GGCGGA 1 cut(s) 2453
Eco130I CCWWGG 3 cut(s) 262, 1297, 1872
Eco147I AGGCCT 1 cut(s) 1842
Eco31I GGTCTC 1 cut(s) 263
Eco32I GATATC 1 cut(s) 1459
Eco47I GGWCC 3 cut(s) 168, 1280, 2186
Eco81I CCTNAGG 1 cut(s) 2682
EcoO109I RGGNCCY 3 cut(s) 757, 971, 2592
EcoRII CCWGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
EcoRV GATATC 1 cut(s) 1459
EcoT14I CCWWGG 3 cut(s) 262, 1297, 1872
ErhI CCWWGG 3 cut(s) 262, 1297, 1872
FaqI GGGAC 4 cut(s) 1799, 2199, 2243, 2563
FbaI TGATCA 1 cut(s) 2206
Fnu4HI GCNGC 4 cut(s) 1260, 2239, 2702, 3261
FokI GGATG 9 cut(s) 401, 1078, 1199, 1588, 1849, 2323, 3173, 3212, 3299
Fsp4HI GCNGC 4 cut(s) 1260, 2239, 2702, 3261
GluI GCNGC 4 cut(s) 1260, 2239, 2702, 3261
GsuI CTGGAG 2 cut(s) 1380, 3117
HapII CCGG 3 cut(s) 624, 1878, 2638
HgaI GACGC 2 cut(s) 1001, 2951
Hin1I GRCGYC 1 cut(s) 1012
HincII GTYRAC 1 cut(s) 2197
HindII GTYRAC 1 cut(s) 2197
HindIII AAGCTT 4 cut(s) 181, 443, 1904, 1984
HinfI GANTC 9 cut(s) 201, 228, 504, 1377, 1790, 2173, 2256, 2647, 3209
HpaII CCGG 3 cut(s) 624, 1878, 2638
HphI GGTGA 5 cut(s) 82, 613, 1705, 2180, 2415
Hpy166II GTNNAC 5 cut(s) 1035, 1696, 1783, 2197, 2527
Hpy8I GTNNAC 5 cut(s) 1035, 1696, 1783, 2197, 2527
HpyCH4III ACNGT 8 cut(s) 151, 564, 1710, 2190, 2370, 2756, 2839, 2971
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 369, 915, 2900
HpyF3I CTNAG 8 cut(s) 476, 1373, 1530, 1732, 1763, 2123, 2682, 2759
Hsp92I GRCGYC 1 cut(s) 1012
Ksp22I TGATCA 1 cut(s) 2206
LguI GCTCTTC 2 cut(s) 111, 879
LmnI GCTCC 1 cut(s) 1678
Lsp1109I GCAGC 3 cut(s) 1246, 2688, 3272
MaeIII GTNAC 7 cut(s) 229, 392, 410, 683, 938, 2421, 3103
MfeI CAATTG 5 cut(s) 861, 1536, 1608, 2331, 2553
MflI RGATCY 3 cut(s) 712, 741, 2071
MhlI GDGCHC 4 cut(s) 343, 2099, 2439, 3297
MlsI TGGCCA 2 cut(s) 71, 774
MluNI TGGCCA 2 cut(s) 71, 774
MlyI GAGTC 3 cut(s) 210, 237, 2265
MmeI TCCRAC 3 cut(s) 108, 384, 2040
Mox20I TGGCCA 2 cut(s) 71, 774
MscI TGGCCA 2 cut(s) 71, 774
MslI CAYNNNNRTG 2 cut(s) 1504, 2850
Msp20I TGGCCA 2 cut(s) 71, 774
MspI CCGG 3 cut(s) 624, 1878, 2638
MspR9I CCNGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
MunI CAATTG 5 cut(s) 861, 1536, 1608, 2331, 2553
Mva1269I GAATGC 2 cut(s) 803, 2621
MvaI CCWGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
MwoI GCNNNNNNNGC 4 cut(s) 110, 369, 915, 2900
NcoI CCATGG 1 cut(s) 1872
NlaIV GGNNCC 9 cut(s) 268, 758, 919, 972, 1832, 1940, 2187, 2593, 3294
NmuCI GTSAC 3 cut(s) 229, 2421, 3103
NspI RCATGY 8 cut(s) 946, 1090, 2017, 2673, 2713, 2785, 2900, 3199
NspV TTCGAA 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 1504
PagI TCATGA 1 cut(s) 1572
PceI AGGCCT 1 cut(s) 1842
PciI ACATGT 7 cut(s) 942, 1086, 2013, 2669, 2709, 2896, 3195
PciSI GCTCTTC 2 cut(s) 111, 879
PctI GAATGC 2 cut(s) 803, 2621
PfeI GAWTC 6 cut(s) 504, 1377, 1790, 2173, 2647, 3209
PflFI GACNNNGTC 1 cut(s) 1807
PflMI CCANNNNNTGG 1 cut(s) 2993
PfoI TCCNGGA 1 cut(s) 3228
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 4 cut(s) 1261, 2240, 2703, 3262
PleI GAGTC 3 cut(s) 209, 236, 2264
PpsI GAGTC 3 cut(s) 209, 236, 2264
PscI ACATGT 7 cut(s) 942, 1086, 2013, 2669, 2709, 2896, 3195
PshAI GACNNNNGTC 1 cut(s) 2910
PsiI TTATAA 1 cut(s) 831
Psp6I CCWGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
PspGI CCWGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
PspN4I GGNNCC 9 cut(s) 268, 758, 919, 972, 1832, 1940, 2187, 2593, 3294
PspPI GGNCC 6 cut(s) 168, 757, 971, 1280, 2186, 2592
PsrI GAACNNNNNNTAC 2 cut(s) 2950, 2982
PstI CTGCAG 2 cut(s) 48, 2706
PsuI RGATCY 3 cut(s) 712, 741, 2071
PsyI GACNNNGTC 1 cut(s) 1807
RsaI GTAC 5 cut(s) 50, 2232, 2528, 2708, 2968
RsaNI GTAC 5 cut(s) 49, 2231, 2527, 2707, 2967
RseI CAYNNNNRTG 2 cut(s) 1504, 2850
SapI GCTCTTC 2 cut(s) 111, 879
SatI GCNGC 4 cut(s) 1260, 2239, 2702, 3261
Sau96I GGNCC 6 cut(s) 168, 757, 971, 1280, 2186, 2592
ScaI AGTACT 2 cut(s) 50, 2232
SchI GAGTC 3 cut(s) 210, 237, 2265
ScrFI CCNGG 9 cut(s) 1316, 1359, 1503, 1517, 1700, 1835, 2354, 2862, 3230
SduI GDGCHC 4 cut(s) 343, 2099, 2439, 3297
SfcI CTRYAG 3 cut(s) 44, 2702, 3000
SfuI TTCGAA 1 cut(s) 319
SinI GGWCC 3 cut(s) 168, 1280, 2186
SmiMI CAYNNNNRTG 2 cut(s) 1504, 2850
SmlI CTYRAG 7 cut(s) 62, 373, 784, 1071, 2726, 3241, 3302
SmoI CTYRAG 7 cut(s) 62, 373, 784, 1071, 2726, 3241, 3302
SseBI AGGCCT 1 cut(s) 1842
SsiI CCGC 4 cut(s) 159, 2238, 2464, 2487
SspI AATATT 4 cut(s) 580, 850, 1657, 2066
StuI AGGCCT 1 cut(s) 1842
StyD4I CCNGG 9 cut(s) 1314, 1357, 1501, 1515, 1698, 1833, 2352, 2860, 3228
StyI CCWWGG 3 cut(s) 262, 1297, 1872
TaaI ACNGT 8 cut(s) 151, 564, 1710, 2190, 2370, 2756, 2839, 2971
TaqI TCGA 5 cut(s) 135, 312, 319, 947, 2790
TaqII GACCGA 1 cut(s) 2900
TatI WGTACW 4 cut(s) 48, 2230, 2526, 2706
TauI GCSGC 1 cut(s) 2241
TfiI GAWTC 6 cut(s) 504, 1377, 1790, 2173, 2647, 3209
TscAI CASTG 5 cut(s) 348, 1594, 1824, 2050, 2209
TseFI GTSAC 3 cut(s) 229, 2421, 3103
TseI GCWGC 3 cut(s) 1259, 2701, 3260
Tsp45I GTSAC 3 cut(s) 229, 2421, 3103
TspGWI ACGGA 1 cut(s) 2404
TspRI CASTG 5 cut(s) 348, 1594, 1824, 2050, 2209
Tth111I GACNNNGTC 1 cut(s) 1807
Van91I CCANNNNNTGG 1 cut(s) 2993
VpaK11BI GGWCC 3 cut(s) 168, 1280, 2186
XapI RAATTY 9 cut(s) 777, 1552, 1727, 1822, 1855, 2143, 2248, 2575, 2922
XbaI TCTAGA 2 cut(s) 493, 3148
XceI RCATGY 8 cut(s) 946, 1090, 2017, 2673, 2713, 2785, 2900, 3199
XcmI CCANNNNNNNNNTGG 3 cut(s) 151, 1824, 2968
ZrmI AGTACT 2 cut(s) 50, 2232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.