Rw4G009540

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000058
Physical Location & Seq
Forward (+)
21520577 .. 21524620
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009540.1

Sequence Viewer

Length: 3375 bp
ATGCACATTGCTACTCTCACCATGGGAAACCCACAGATACTCGTACTCGTACCGTATGGACTAGTTGTGTTACTTCACTTCTCATGCTTCCTGCAGTTAACCTCATCTTCTTCCACTTCCAACTTCACAGATCAAACTGCTCTTCTTGCCATTCAATCCAAACTCACATTTGATCCTACTGAAACTGTCTTGGGTGGCAATTGGACCACCAACACAAGCTTCTGTGATTGGATTGGGGTCTCTTGCGGTAATCGAAGGCAAAGAGTCATCGCCTTGGACCTGTCATATATGGGCCTCCAAGGCACAATTGCACCACATGTTGGCAACCTCTCCTTTCTGGTTTCATTGGATCTCCGAAATAACAGCTTCTCTGGGTTCCTGCCGAATGAAATCAGCCGTTTACATTGCTTGAAGGTACTCATGCTGTATGCCAACCAGTTGGAGGGAAACATCCCTCCAACTTTGTATCGCTGTGAAAAGCTTGAAATTATTAGCCTTGCAATAAACAAGCTTAGCGGCCATATACCTGAAGAGTTGGCTTTCTTACCCAGACTTGAGCAACTATTTCTTGTTCACAATAATTTGTCTGGTGGAGTTCCACCAATTTTGGGTAACATTTCAACATTAGTAGTATTGGATATGTCCTATAACGGCCTTACTGGTTCACTTCCTTCTTCCCTGTTTAACCTGTCTTCTTTAGGTAGTCTTTCTGTGCACCAAAATTTCCTTTCTGGAAGTTTTCTTGATGTTGATCTCTGCCGTTCTTGGCCTAGACTTCAATTCCTTTCTCTTACTCTCAATAATTTCAGTGGCCGGCTTCCTAACCTCAGCAATTGTACAGAGCTTGTCATATTGTCTTTATCACATAATAAGTTTGTGGGAAAAATTCCAGCAGATATTGGCAACTTCAAAAACCTTGAAGAACTTTATCTTGATTTTAATAGCCTCACTGGTACTATACCTCCTACCATAGGTAACTTGTCCAGCTTGAAATCCTTGGGTTTAACGCAGAATAACGTTAAGGGAAGCATACCTCGTGATTTAGGACGTCTTTCTAGATTGGTCAAATTTTTGATTTGTAGGAATGGCCTAAGCGGACCAGTGCCTCCAGAAATATTCAATATCTCTTCTCTGCAAGTTATTGAAGCATGCGATAATGCCCTTTCAGGAAAGTTTCCTTCCTCTTCTGCAGTTCGGCTTCCTAGCATTGAATTCATCTCTTTTCCTCACAATAAAATTACTGGACATGTCCCATCCTATTTTGCAAATTTTACAAAGCTTTACTCACTTCAGGTTGGATACAACTTACTCTACGGGCCACTTCCCAAGAATTTTGGTAGCTTAAACCACCTTGAAACCCTTCATCTCGGGGGAAATCAATTAACAGGAGATCCAGGAGTTCCTACACTTGATTTTCTTTCTTCTTTGTTCAATTGCCCTTCACTGAAAGAATTAGTTCTGTCTGAGAATCCTTTAAATGGCACCATACCAAACTCCTTTGGACATTCTTCTTCTCCTCTCCAGTTGTTTTTAATAGACAAGTGCCAGATTAAGGGCCACATACCCAAGGGAATTGGTTCTTTGAGAAGCTTGACGTTCCTTTCATGGGATAACAACAATCTGAGTGGAAATATACCAGAGTCAATTGGAGGTTTGGAGAGGCTGCAAAGATTGTATCTTGGCAACAACAGTTTAGAAGGCTCTATTCCAGAAGAGCTTTGTCTTTTGAGGAACCTGGGTGAACTATCACTACAAAATAATAAAATCTCTGAATCAATCCCAACTTGCATAGGAAACCTCATCCACCTTCAGAGTGTTTTCCTAAGTTCCAATAGATTGACATCATCAGTACCGTTGGGTTTATGGGACCTTGAAAATCTCCTGTTCCTAGATCTGTCAATCAATTCTTTTTATGGGAACTTACCTCCAAGCTTGAGCAAGTTGAATGCTATTCAAGAGATAGATTTATCTCAAAACCAATTTACCGGAAACATTTCAAGCACCATAGGCAGTTTGCAAACTCTGAGCTCTCTTAATCTGTCACACAACTTCTTTACAGGTTCTGTTCCACAAACTTTTTGTGATAAGCTGGGGTTGGAATTGTTGGACTTATCATTCAATAATCTTTCTGGTGCAATACCAATGTGTCTTGAGAAACTCAAATTTCTCAATTACTTAAATCTCTCGTTCAACTACCTCTCCGGAGAGGTCCCTTCTGGAGGACGTTTCGTCAACTTCAGTGCCCAGTCATTTTTGGAGAATAAAGCACTATGTGGGAGACGAGAGTTTGGAGTCCCACCTTGCACGACTCATAGGACTCAAAAATCAAAAGTGGCGACTCGTTTGCTGGCCAAATATGTTCTTCCAGCCATTGCATCAACACTGATTCTCATAGCCCTCTGTTACATGTTGACAAGGTATGGAAAAAGAAAGAGAGGCAAGACTTCGAGTTCAGCTGAACAGTTAACAACAGTGGAGCATATTGTGATATCATATCAAGAACTCCGTCAGGCTACAAATGACTTCTGTGAAAGAAACATGCTTGGAGTGGGAAGTTTTGGCTCGGTGTACAAGGGGATACTGTCTAATGGGATAACCATTGCTGTCAAAGTTCTTAAGCTGGAAATGGAGGGTGCTGAAAAAAGTTTTGATGCAGAATGCAAGGTGTGGAGAACAAACCGGCATAGGAATCTTGTTAAGGTCATAACTACATGTTCTAGCCCTGAAATAAGAGCTTTGGTGCTGCAGTACATGTCTAATGGCAGTCTTGAGAAGTGGTTATACTCTCACAACTACTGTCTCAGTATCCTGCAAAGAGTGAGCATGTTAATCGATATTGCATCTGCTTTGGAATATCTCCATCATGGTCATGCAGAAACCGTGGTGCACTGTGATGTGAAGCCTGGCAATGTCCTTCTTGATGAAGACATGGTTGCACATCTTGCAGACTTCGGTCTTGCAAAAATTTTAGCAAAAAATAAAGACGCAACACAGACAAGAACCATCGGTACTGTGGGCTATGTAGCGCCAGAGTATGGTTCTGCAGGAAAAGTGTCCAGAAGTGGTGATGTTTATAGCTTCGGGATAATGTTGCTGGAAATAATGGCGAGAAAGAAACCTACTGATGAAATGTTTGCTGGAGAAGTGACTTTGAGGCAATGGGTAAATGTGTCTATCCCTGATAATGTTCTAGAAGTGGTGGATGCTGGCTTATTGAGCTTAGAAGAGGGAAGAGACATGTTTGCCACAGAATCTATCCTTATGTTCATCCTGGAAATAGGCTTGAGGTGTTCCGAAGAAGTAGCAGAGGATAGAATGGACATCAAGGATGTGGTGCCCAAGCTCAAGAAAATAAAAGTGGCACTACAAGAATGA

Protein Analysis

1124

Amino Acids

123.52

Weight (kDa)

6.8

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 92 - 286 2.2e-10 Leucine-rich repeat region
LRR_14 PF23598 268 - 384 4.5e-07 Leucine-rich repeat region
LRR_14 PF23598 512 - 738 3.4e-14 Leucine-rich repeat region
LRR_8 PF13855 674 - 733 8.3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 846 - 1048 1.4e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 846 - 1042 4e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1051
AccB1I GGYRCC 2 cut(s) 1481, 3334
AccB7I CCANNNNNTGG 2 cut(s) 320, 3035
AccIII TCCGGA 1 cut(s) 2201
AciI CCGC 3 cut(s) 246, 516, 1095
AclI AACGTT 1 cut(s) 1017
AclWI GGATC 3 cut(s) 167, 357, 1385
AcoI YGGCCR 3 cut(s) 517, 811, 2349
AcsI RAATTY 8 cut(s) 721, 885, 1067, 1211, 1267, 1330, 2162, 2964
AcuI CTGAAG 4 cut(s) 549, 1274, 1793, 2221
AcyI GRCGYC 1 cut(s) 1048
AdeI CACNNNGTG 1 cut(s) 2273
AfaI GTAC 9 cut(s) 45, 51, 417, 838, 955, 1851, 2570, 2750, 3010
AfiI CCNNNNNNNGG 9 cut(s) 320, 442, 608, 971, 1478, 1552, 1606, 2219, 3035
AflII CTTAAG 1 cut(s) 2615
AflIII ACRYGT 6 cut(s) 316, 1246, 2406, 2711, 2751, 3237
AhdI GACNNNNNGTC 1 cut(s) 2228
AhlI ACTAGT 1 cut(s) 61
AjnI CCWGG 4 cut(s) 1393, 1734, 2902, 3270
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
Alw21I GWGCWC 3 cut(s) 717, 2030, 2889
Alw26I GTCTC 4 cut(s) 244, 2272, 2804, 3228
Alw44I GTGCAC 2 cut(s) 713, 2885
AlwI GGATC 3 cut(s) 167, 357, 1385
AlwNI CAGNNNCTG 1 cut(s) 2063
Ama87I CYCGRG 1 cut(s) 1367
Aor13HI TCCGGA 1 cut(s) 2201
AoxI GGCC 9 cut(s) 292, 517, 652, 767, 811, 1087, 1316, 1555, 2349
ApaLI GTGCAC 2 cut(s) 713, 2885
ApeKI GCWGC 2 cut(s) 1663, 2743
ApoI RAATTY 8 cut(s) 721, 885, 1067, 1211, 1267, 1330, 2162, 2964
Asp700I GAANNNNTTC 4 cut(s) 1359, 1455, 1576, 1993
AspLEI GCGC 1 cut(s) 3028
AspS9I GGNCC 8 cut(s) 204, 277, 292, 1097, 1316, 1555, 1867, 2209
AsuHPI GGTGA 3 cut(s) 10, 1751, 3077
AvaI CYCGRG 1 cut(s) 1367
AvaII GGWCC 5 cut(s) 204, 277, 1097, 1867, 2209
BaeGI GKGCMC 4 cut(s) 717, 2245, 2889, 3339
BalI TGGCCA 1 cut(s) 2351
BanI GGYRCC 2 cut(s) 1481, 3334
BanII GRGCYC 1 cut(s) 2030
BarI GAAGNNNNNNTAC 2 cut(s) 2765, 2797
BauI CACGAG 1 cut(s) 1035
BbsI GAAGAC 2 cut(s) 684, 2931
Bbv12I GWGCWC 3 cut(s) 717, 2030, 2889
BbvCI CCTCAGC 1 cut(s) 827
BbvI GCAGC 2 cut(s) 1650, 2730
BccI CCATC 3 cut(s) 1261, 2868, 3011
BceAI ACGGC 3 cut(s) 381, 667, 744
BcgI CGANNNNNNTGC 4 cut(s) 2326, 2360, 2812, 2846
BciT130I CCWGG 4 cut(s) 1395, 1736, 2904, 3272
BciVI GTATCC 3 cut(s) 1292, 2571, 2816
BcoDI GTCTC 4 cut(s) 244, 2272, 2804, 3228
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 7 cut(s) 62, 771, 1056, 1203, 1889, 2718, 3191
BfmI CTRYAG 4 cut(s) 92, 1188, 2744, 3042
BfoI RGCGCY 1 cut(s) 3029
BfrI CTTAAG 1 cut(s) 2615
BfuI GTATCC 3 cut(s) 1292, 2571, 2816
BglI GCCNNNNNGGC 1 cut(s) 300
BglII AGATCT 1 cut(s) 1891
BisI GCNGC 3 cut(s) 517, 1664, 2744
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 3 cut(s) 518, 1665, 2745
Bme1390I CCNGG 4 cut(s) 1395, 1736, 2904, 3272
Bme18I GGWCC 5 cut(s) 204, 277, 1097, 1867, 2209
BmeRI GACNNNNNGTC 1 cut(s) 2228
BmeT110I CYCGRG 1 cut(s) 1367
BmgT120I GGNCC 8 cut(s) 204, 277, 292, 1097, 1316, 1555, 1867, 2209
BmiI GGNNCC 6 cut(s) 377, 1483, 1733, 1868, 2211, 3336
BmrFI CCNGG 4 cut(s) 1395, 1736, 2904, 3272
BmrI ACTGGG 1 cut(s) 2239
BmsI GCATC 4 cut(s) 2384, 2641, 2849, 3193
BmuI ACTGGG 1 cut(s) 2239
BoxI GACNNNNGTC 1 cut(s) 2952
BpiI GAAGAC 2 cut(s) 684, 2931
BpmI CTGGAG 4 cut(s) 1092, 1505, 2238, 3159
Bpu10I CCTNAGC 2 cut(s) 827, 1091
Bpu1102I GCTNAGC 1 cut(s) 512
BpuEI CTTGAG 6 cut(s) 575, 1954, 2171, 2789, 3304, 3329
Bsa29I ATCGAT 1 cut(s) 2832
BsaBI GATNNNNATC 2 cut(s) 750, 1840
BsaHI GRCGYC 1 cut(s) 1048
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 7 cut(s) 21, 273, 298, 996, 1566, 1735, 2880
BsaWI WCCGGW 2 cut(s) 1985, 2201
BsaXI ACNNNNNCTCC 4 cut(s) 946, 976, 2183, 2213
Bsc4I CCNNNNNNNGG 9 cut(s) 320, 442, 608, 971, 1478, 1552, 1606, 2219, 3035
Bse118I RCCGGY 2 cut(s) 813, 2679
Bse1I ACTGG 7 cut(s) 436, 664, 955, 1100, 1246, 1522, 2245
Bse3DI GCAATG 6 cut(s) 6, 403, 2370, 2598, 2914, 3164
Bse8I GATNNNNATC 2 cut(s) 750, 1840
BseAI TCCGGA 1 cut(s) 2201
BseBI CCWGG 4 cut(s) 1395, 1736, 2904, 3272
BseCI ATCGAT 1 cut(s) 2832
BseDI CCNNGG 7 cut(s) 21, 273, 298, 996, 1566, 1735, 2880
BseGI GGATG 6 cut(s) 450, 1253, 1800, 3208, 3267, 3334
BseJI GATNNNNATC 2 cut(s) 750, 1840
BseLI CCNNNNNNNGG 9 cut(s) 320, 442, 608, 971, 1478, 1552, 1606, 2219, 3035
BseMI GCAATG 6 cut(s) 6, 403, 2370, 2598, 2914, 3164
BseMII CTCAG 5 cut(s) 841, 1455, 1613, 2015, 2815
BseNI ACTGG 7 cut(s) 436, 664, 955, 1100, 1246, 1522, 2245
BseRI GAGGAG 1 cut(s) 1506
BseSI GKGCMC 4 cut(s) 717, 2245, 2889, 3339
BseXI GCAGC 2 cut(s) 1650, 2730
BseYI CCCAGC 1 cut(s) 2089
BshFI GGCC 9 cut(s) 294, 519, 654, 769, 813, 1089, 1318, 1557, 2351
BshNI GGYRCC 2 cut(s) 1481, 3334
BshVI ATCGAT 1 cut(s) 2832
BsiHKAI GWGCWC 3 cut(s) 717, 2030, 2889
BsiHKCI CYCGRG 1 cut(s) 1367
BsiSI CCGG 4 cut(s) 814, 1986, 2202, 2680
BslFI GGGAC 4 cut(s) 1235, 1880, 2195, 2279
BslI CCNNNNNNNGG 9 cut(s) 320, 442, 608, 971, 1478, 1552, 1606, 2219, 3035
BsmAI GTCTC 4 cut(s) 244, 2272, 2804, 3228
BsmBI CGTCTC 1 cut(s) 2272
BsmFI GGGAC 4 cut(s) 1235, 1880, 2195, 2279
BsmI GAATGC 2 cut(s) 1951, 2663
BsnI GGCC 9 cut(s) 294, 519, 654, 769, 813, 1089, 1318, 1557, 2351
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 1367
Bsp1286I GDGCHC 5 cut(s) 717, 2030, 2245, 2889, 3339
Bsp13I TCCGGA 1 cut(s) 2201
Bsp1407I TGTACA 2 cut(s) 836, 2568
Bsp143I GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
Bsp1720I GCTNAGC 1 cut(s) 512
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 246, 516, 1095
BspANI GGCC 9 cut(s) 294, 519, 654, 769, 813, 1089, 1318, 1557, 2351
BspCNI CTCAG 5 cut(s) 840, 1456, 1614, 2016, 2814
BspDI ATCGAT 1 cut(s) 2832
BspEI TCCGGA 1 cut(s) 2201
BspLI GGNNCC 6 cut(s) 377, 1483, 1733, 1868, 2211, 3336
BspMAI CTGCAG 4 cut(s) 96, 1192, 2748, 3046
BspPI GGATC 3 cut(s) 167, 357, 1385
BspQI GCTCTTC 2 cut(s) 147, 1707
BspT107I GGYRCC 2 cut(s) 1481, 3334
BspTI CTTAAG 1 cut(s) 2615
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 6 cut(s) 6, 403, 2370, 2598, 2914, 3164
BsrFI RCCGGY 2 cut(s) 813, 2679
BsrGI TGTACA 2 cut(s) 836, 2568
BsrI ACTGG 7 cut(s) 436, 664, 955, 1100, 1246, 1522, 2245
BssAI RCCGGY 2 cut(s) 813, 2679
BssECI CCNNGG 7 cut(s) 21, 273, 298, 996, 1566, 1735, 2880
BssMI GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
BssNI GRCGYC 1 cut(s) 1048
BssSI CACGAG 1 cut(s) 1035
BssT1I CCWWGG 5 cut(s) 21, 273, 298, 996, 1566
Bst2BI CACGAG 1 cut(s) 1035
Bst2UI CCWGG 4 cut(s) 1395, 1736, 2904, 3272
Bst6I CTCTTC 7 cut(s) 147, 525, 1132, 1189, 1707, 3219, 3226
BstACI GRCGYC 1 cut(s) 1048
BstAFI CTTAAG 1 cut(s) 2615
BstAPI GCANNNNNTGC 1 cut(s) 2942
BstAUI TGTACA 2 cut(s) 836, 2568
BstC8I GCNNGC 4 cut(s) 815, 1150, 2349, 3208
BstDEI CTNAG 9 cut(s) 512, 827, 1091, 1464, 1622, 1823, 2024, 2801, 3220
BstDSI CCRYGG 2 cut(s) 21, 2880
BstENI CCTNNNNNAGG 2 cut(s) 969, 2217
BstF5I GGATG 6 cut(s) 450, 1253, 1800, 3208, 3267, 3334
BstH2I RGCGCY 1 cut(s) 3029
BstHHI GCGC 1 cut(s) 3028
BstKTI GATC 6 cut(s) 133, 175, 352, 754, 1393, 1894
BstMAI GTCTC 4 cut(s) 244, 2272, 2804, 3228
BstMBI GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
BstMWI GCNNNNNNNGC 5 cut(s) 146, 300, 2007, 2942, 3216
BstNI CCWGG 4 cut(s) 1395, 1736, 2904, 3272
BstNSI RCATGY 9 cut(s) 320, 1152, 1250, 2410, 2542, 2715, 2755, 2827, 3241
BstPAI GACNNNNGTC 1 cut(s) 2952
BstSCI CCNGG 4 cut(s) 1393, 1734, 2902, 3270
BstSFI CTRYAG 4 cut(s) 92, 1188, 2744, 3042
BstSLI GKGCMC 4 cut(s) 717, 2245, 2889, 3339
BstV1I GCAGC 2 cut(s) 1650, 2730
BstV2I GAAGAC 2 cut(s) 684, 2931
BstX2I RGATCY 3 cut(s) 349, 1390, 1891
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 3 cut(s) 349, 1390, 1891
Bsu15I ATCGAT 1 cut(s) 2832
BsuI GTATCC 3 cut(s) 1292, 2571, 2816
BsuRI GGCC 9 cut(s) 294, 519, 654, 769, 813, 1089, 1318, 1557, 2351
BsuTUI ATCGAT 1 cut(s) 2832
BtgI CCRYGG 2 cut(s) 21, 2880
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 6 cut(s) 450, 1253, 1800, 3208, 3267, 3334
BtsIMutI CAGTG 8 cut(s) 814, 948, 1107, 1442, 2245, 2381, 2478, 2887
Cac8I GCNNGC 4 cut(s) 815, 1150, 2349, 3208
CaiI CAGNNNCTG 1 cut(s) 2063
CfoI GCGC 1 cut(s) 3028
Cfr10I RCCGGY 2 cut(s) 813, 2679
Cfr13I GGNCC 8 cut(s) 204, 277, 292, 1097, 1316, 1555, 1867, 2209
ClaI ATCGAT 1 cut(s) 2832
CseI GACGC 1 cut(s) 2993
Csp6I GTAC 9 cut(s) 44, 50, 416, 837, 954, 1850, 2569, 2749, 3009
CspCI CAANNNNNGTGG 4 cut(s) 588, 623, 1606, 1641
CviQI GTAC 9 cut(s) 44, 50, 416, 837, 954, 1850, 2569, 2749, 3009
DdeI CTNAG 9 cut(s) 512, 827, 1091, 1464, 1622, 1823, 2024, 2801, 3220
DpnI GATC 6 cut(s) 132, 174, 351, 753, 1392, 1893
DpnII GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
DraI TTTAAA 1 cut(s) 1476
DraIII CACNNNGTG 1 cut(s) 2273
DriI GACNNNNNGTC 1 cut(s) 2228
EaeI YGGCCR 3 cut(s) 517, 811, 2349
Eam1104I CTCTTC 7 cut(s) 147, 525, 1132, 1189, 1707, 3219, 3226
Eam1105I GACNNNNNGTC 1 cut(s) 2228
EarI CTCTTC 7 cut(s) 147, 525, 1132, 1189, 1707, 3219, 3226
Ecl136II GAGCTC 1 cut(s) 2028
Eco130I CCWWGG 5 cut(s) 21, 273, 298, 996, 1566
Eco24I GRGCYC 1 cut(s) 2030
Eco31I GGTCTC 1 cut(s) 244
Eco32I GATATC 1 cut(s) 2490
Eco47I GGWCC 5 cut(s) 204, 277, 1097, 1867, 2209
Eco53kI GAGCTC 1 cut(s) 2028
Eco57I CTGAAG 4 cut(s) 549, 1274, 1793, 2221
Eco88I CYCGRG 1 cut(s) 1367
EcoICRI GAGCTC 1 cut(s) 2028
EcoNI CCTNNNNNAGG 2 cut(s) 969, 2217
EcoO109I RGGNCCY 2 cut(s) 1867, 2209
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 4 cut(s) 1393, 1734, 2902, 3270
EcoRV GATATC 1 cut(s) 2490
EcoT14I CCWWGG 5 cut(s) 21, 273, 298, 996, 1566
EcoT38I GRGCYC 1 cut(s) 2030
ErhI CCWWGG 5 cut(s) 21, 273, 298, 996, 1566
Esp3I CGTCTC 1 cut(s) 2272
FaqI GGGAC 4 cut(s) 1235, 1880, 2195, 2279
Fnu4HI GCNGC 3 cut(s) 517, 1664, 2744
FokI GGATG 6 cut(s) 437, 1240, 1787, 3215, 3254, 3341
FriOI GRGCYC 1 cut(s) 2030
Fsp4HI GCNGC 3 cut(s) 517, 1664, 2744
FspBI CTAG 7 cut(s) 62, 771, 1056, 1203, 1889, 2718, 3191
GlaI GCGC 1 cut(s) 3027
GluI GCNGC 3 cut(s) 517, 1664, 2744
GsaI CCCAGC 1 cut(s) 2093
GsuI CTGGAG 4 cut(s) 1092, 1505, 2238, 3159
HaeII RGCGCY 1 cut(s) 3029
HaeIII GGCC 9 cut(s) 294, 519, 654, 769, 813, 1089, 1318, 1557, 2351
HapII CCGG 4 cut(s) 814, 1986, 2202, 2680
HgaI GACGC 1 cut(s) 2993
HhaI GCGC 1 cut(s) 3028
Hin1I GRCGYC 1 cut(s) 1048
Hin6I GCGC 1 cut(s) 3026
HinP1I GCGC 1 cut(s) 3026
HincII GTYRAC 4 cut(s) 99, 2233, 2412, 2466
HindII GTYRAC 4 cut(s) 99, 2233, 2412, 2466
HindIII AAGCTT 6 cut(s) 217, 479, 509, 1277, 1588, 1930
HpaI GTTAAC 2 cut(s) 99, 2466
HpaII CCGG 4 cut(s) 814, 1986, 2202, 2680
HphI GGTGA 3 cut(s) 10, 1751, 3077
Hpy188I TCNGA 7 cut(s) 356, 1465, 1623, 1771, 1812, 2025, 3295
HpyCH4IV ACGT 4 cut(s) 1017, 1048, 1595, 2224
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 300, 2007, 2942, 3216
HpyF3I CTNAG 9 cut(s) 512, 827, 1091, 1464, 1622, 1823, 2024, 2801, 3220
HpySE526I ACGT 4 cut(s) 1017, 1048, 1595, 2224
Hsp92I GRCGYC 1 cut(s) 1048
HspAI GCGC 1 cut(s) 3026
Kpn2I TCCGGA 1 cut(s) 2201
KroI GCCGGC 1 cut(s) 813
KroNI GCCGGC 1 cut(s) 815
KspAI GTTAAC 2 cut(s) 99, 2466
Kzo9I GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
LguI GCTCTTC 2 cut(s) 147, 1707
LmnI GCTCC 1 cut(s) 2476
Lsp1109I GCAGC 2 cut(s) 1650, 2730
LweI GCATC 4 cut(s) 2384, 2641, 2849, 3193
MaeI CTAG 7 cut(s) 62, 771, 1056, 1203, 1889, 2718, 3191
MaeII ACGT 4 cut(s) 1017, 1048, 1595, 2224
MaeIII GTNAC 6 cut(s) 69, 611, 974, 2040, 2402, 3145
MalI GATC 6 cut(s) 132, 174, 351, 753, 1392, 1893
MboI GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
MfeI CAATTG 5 cut(s) 199, 306, 832, 1432, 1644
MflI RGATCY 3 cut(s) 349, 1390, 1891
MhlI GDGCHC 5 cut(s) 717, 2030, 2245, 2889, 3339
MlsI TGGCCA 1 cut(s) 2351
MluNI TGGCCA 1 cut(s) 2351
MlyI GAGTC 6 cut(s) 273, 1649, 2301, 2302, 2311, 2332
MmeI TCCRAC 6 cut(s) 144, 420, 482, 1276, 2076, 2085
Mox20I TGGCCA 1 cut(s) 2351
MroI TCCGGA 1 cut(s) 2201
MroNI GCCGGC 1 cut(s) 813
MroXI GAANNNNTTC 4 cut(s) 1359, 1455, 1576, 1993
MscI TGGCCA 1 cut(s) 2351
MslI CAYNNNNRTG 2 cut(s) 2868, 2892
Msp20I TGGCCA 1 cut(s) 2351
MspA1I CMGCKG 1 cut(s) 2456
MspCI CTTAAG 1 cut(s) 2615
MspI CCGG 4 cut(s) 814, 1986, 2202, 2680
MspR9I CCNGG 4 cut(s) 1395, 1736, 2904, 3272
MunI CAATTG 5 cut(s) 199, 306, 832, 1432, 1644
Mva1269I GAATGC 2 cut(s) 1951, 2663
MvaI CCWGG 4 cut(s) 1395, 1736, 2904, 3272
MwoI GCNNNNNNNGC 5 cut(s) 146, 300, 2007, 2942, 3216
NaeI GCCGGC 1 cut(s) 815
NcoI CCATGG 1 cut(s) 21
NdeII GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
NgoMIV GCCGGC 1 cut(s) 813
NlaIV GGNNCC 6 cut(s) 377, 1483, 1733, 1868, 2211, 3336
NmuCI GTSAC 2 cut(s) 2040, 3145
NspI RCATGY 9 cut(s) 320, 1152, 1250, 2410, 2542, 2715, 2755, 2827, 3241
PaeI GCATGC 1 cut(s) 1152
PciI ACATGT 6 cut(s) 316, 1246, 2406, 2711, 2751, 3237
PciSI GCTCTTC 2 cut(s) 147, 1707
PctI GAATGC 2 cut(s) 1951, 2663
PdiI GCCGGC 1 cut(s) 815
PdmI GAANNNNTTC 4 cut(s) 1359, 1455, 1576, 1993
PfeI GAWTC 5 cut(s) 1468, 1772, 2386, 2689, 3251
PflMI CCANNNNNTGG 2 cut(s) 320, 3035
PfoI TCCNGGA 2 cut(s) 1393, 3270
PkrI GCNGC 3 cut(s) 518, 1665, 2745
PleI GAGTC 6 cut(s) 272, 1648, 2300, 2302, 2311, 2332
PpsI GAGTC 6 cut(s) 272, 1648, 2300, 2302, 2311, 2332
PpuMI RGGWCCY 2 cut(s) 1867, 2209
PscI ACATGT 6 cut(s) 316, 1246, 2406, 2711, 2751, 3237
PshAI GACNNNNGTC 1 cut(s) 2952
Psp124BI GAGCTC 1 cut(s) 2030
Psp1406I AACGTT 1 cut(s) 1017
Psp5II RGGWCCY 2 cut(s) 1867, 2209
Psp6I CCWGG 4 cut(s) 1393, 1734, 2902, 3270
PspFI CCCAGC 1 cut(s) 2089
PspGI CCWGG 4 cut(s) 1393, 1734, 2902, 3270
PspN4I GGNNCC 6 cut(s) 377, 1483, 1733, 1868, 2211, 3336
PspPI GGNCC 8 cut(s) 204, 277, 292, 1097, 1316, 1555, 1867, 2209
PspPPI RGGWCCY 2 cut(s) 1867, 2209
PsrI GAACNNNNNNTAC 4 cut(s) 1734, 1766, 2992, 3024
PstI CTGCAG 4 cut(s) 96, 1192, 2748, 3046
PstNI CAGNNNCTG 1 cut(s) 2063
PsuI RGATCY 3 cut(s) 349, 1390, 1891
PvuII CAGCTG 1 cut(s) 2456
RsaI GTAC 9 cut(s) 45, 51, 417, 838, 955, 1851, 2570, 2750, 3010
RsaNI GTAC 9 cut(s) 44, 50, 416, 837, 954, 1850, 2569, 2749, 3009
RseI CAYNNNNRTG 2 cut(s) 2868, 2892
SacI GAGCTC 1 cut(s) 2030
SapI GCTCTTC 2 cut(s) 147, 1707
SatI GCNGC 3 cut(s) 517, 1664, 2744
Sau3AI GATC 6 cut(s) 130, 172, 349, 751, 1390, 1891
Sau96I GGNCC 8 cut(s) 204, 277, 292, 1097, 1316, 1555, 1867, 2209
SchI GAGTC 6 cut(s) 273, 1649, 2301, 2302, 2311, 2332
ScrFI CCNGG 4 cut(s) 1395, 1736, 2904, 3272
SduI GDGCHC 5 cut(s) 717, 2030, 2245, 2889, 3339
SfaNI GCATC 4 cut(s) 2384, 2641, 2849, 3193
SfcI CTRYAG 4 cut(s) 92, 1188, 2744, 3042
SinI GGWCC 5 cut(s) 204, 277, 1097, 1867, 2209
SmiMI CAYNNNNRTG 2 cut(s) 2868, 2892
SmlI CTYRAG 7 cut(s) 554, 1933, 2150, 2615, 2768, 3283, 3344
SmoI CTYRAG 7 cut(s) 554, 1933, 2150, 2615, 2768, 3283, 3344
SpeI ACTAGT 1 cut(s) 61
SphI GCATGC 1 cut(s) 1152
SsiI CCGC 3 cut(s) 246, 516, 1095
SspI AATATT 1 cut(s) 1116
SspMI CTAG 7 cut(s) 62, 771, 1056, 1203, 1889, 2718, 3191
SstI GAGCTC 1 cut(s) 2030
StyD4I CCNGG 4 cut(s) 1393, 1734, 2902, 3270
StyI CCWWGG 5 cut(s) 21, 273, 298, 996, 1566
TaiI ACGT 4 cut(s) 1020, 1051, 1598, 2227
TaqI TCGA 3 cut(s) 253, 2447, 2832
TaqII GACCGA 1 cut(s) 2942
TatI WGTACW 3 cut(s) 836, 2568, 2748
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 5 cut(s) 1468, 1772, 2386, 2689, 3251
TscAI CASTG 8 cut(s) 814, 955, 1107, 1449, 2245, 2388, 2478, 2894
TseFI GTSAC 2 cut(s) 2040, 3145
TseI GCWGC 2 cut(s) 1663, 2743
Tsp45I GTSAC 2 cut(s) 2040, 3145
TspDTI ATGAA 8 cut(s) 333, 402, 1204, 1352, 1593, 2937, 3141, 3256
TspGWI ACGGA 1 cut(s) 2495
TspRI CASTG 8 cut(s) 814, 955, 1107, 1449, 2245, 2388, 2478, 2894
Van91I CCANNNNNTGG 2 cut(s) 320, 3035
Vha464I CTTAAG 1 cut(s) 2615
VneI GTGCAC 2 cut(s) 713, 2885
VpaK11BI GGWCC 5 cut(s) 204, 277, 1097, 1867, 2209
XagI CCTNNNNNAGG 2 cut(s) 969, 2217
XapI RAATTY 8 cut(s) 721, 885, 1067, 1211, 1267, 1330, 2162, 2964
XbaI TCTAGA 2 cut(s) 1055, 3190
XceI RCATGY 9 cut(s) 320, 1152, 1250, 2410, 2542, 2715, 2755, 2827, 3241
XcmI CCANNNNNNNNNTGG 1 cut(s) 3010
XmnI GAANNNNTTC 4 cut(s) 1359, 1455, 1576, 1993
XspI CTAG 7 cut(s) 62, 771, 1056, 1203, 1889, 2718, 3191
ZraI GACGTC 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.