RchiOBHm_Chr4g0403091

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000058
Physical Location & Seq
Forward (+)
22121335 .. 22125133
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37486

Sequence Viewer

Length: 3261 bp
ATGGGAAACCCACAAATGTTCATGCCATTTGGACTAGTTGTGTTACTTCACTACGCATGCTTCCTTCACTCGGGCACCTCCTCTACTTCCAACTTCACTGATCTAACTGCTCTTCTTGCAATTCAATCCAAACTCACATTTGAACCTACCAAAACTGTCTTGGGTGGCAATTGGACCACCAACACAAGCTTCTGTGATTGGATTGGGGTCTCTTGCAGTAAACGAAGGCAAAGAGTCACTGCCTTGGACCTGTCATATATGGGTCTCCAAGGCACAATTGCACCACATGTTGGCAACCTCTCCTTTCTGGTCTCATTGGATCTCCGAAATAACAGCTTCTCTGGGTTCCTGCCAAATGAAATCAGCCATTTACATCGCTTGAAGGTACTCATGCTGCATGCCAACCAGTTGGAGGGAAACATTCCTCCAACTTTGTATCGCTGCGAAAAGCTTGAACTGATTAGCCTTGCAATAAACAAGCTTAGCGGCCATATACCTAAAGAGTTGGGTTTCTTACCCAGACTTGAGCAACTATTGATTGGTAACAATAATTTTTCTGGTGGAGTTCCACCAATTTTGGGTAACATTTCAACATTAGAAGTATTGAATATGTCCTATAACGGCCTTACTGGTTCACTTCCTTCTTCCCTGTTTAACCTGTCTTCTTTAGTTACTTTTTCTGTGGAACAAAATCACCTTTCTGGAAGTTTTCATGATGTTGATGTCTGCCATTCTTGGCCTAGACTTCAATTCCTTTCTCTTGTTCACAATAATTTTAGTGGCCAGCTTCCTTACTTCAGAAATTGTACAGAGCTTGTCATCTTGTATTTATCATTCAATAAGTTTGTGGGAAAAATTCCAGCAGATATTGGCAACTTACAAAACCTTGAGGAACTTTATCTTAGTTTTAATAGCCTAACTGGTACTATACCTCCTAGCATAGGTAACTTGTCCAGCTTGAGATCCTTGCTTTTAAGGGAGAATAACCTTAAGGGAAGCATTCCTCGTGATTCAGGACGACTTTCTAGATTGGTCTTTTTCGTGATTCGTACGAATGGCCTAAGCGGACCAGTGCCTCTGGAAATATTCAATATCTCCTCCCTGCAAGTTATTGAAGCAAAAGATAATGCCCTTTCAGGAAAGTTTCCTTCCTCCTCTGCAGTTCGGCTTCCTAGCATTGGATTCATCACTCTTTCTCACAATAAAATTACCGGACATGTCCCATCCTATTTTGCAAATTTTACAAAGCTCTACGTATTTCAGGTTACATCCAACTTTCTCTACGGACCAATTCCCAAGAATTTTGGTAGCTTAAATCACCTCGAAAACCTTCTTCTCGGGGGAAATCAATTAACAGGAGATCCCGGAGTTCCTACACTTGATTTTCTTTCTTCTTTGTTTAATGGCCCTTCCCTGAAAAGATTAGTTCTGTCTGAGAATCCTTTAAATGGCACCATACCAAACTCCTTTGGAAATTCTTCTTCTCCTCTCCAGTTCTTTTGGATAGACAAGTGCCAGATTAAGGGCCACATACCCAAGGGAATTGGTTCTTTGAAAAGCTTGACGTACCTTTTATGGGGTAACAACAATCTGAGTGGAAATATACCAGAGTCAATTGGAGGTTTGGAGAGGCTGCAAAGATTGTATCTTGGCAACAACAGTTTAGAAGGCTGTATTCCAGACGAGCTTTGTCTTTTGAGGAACCTGGGAGAACTATCACTACAAAACAATAAAATCTCTGAATCAATCCCAAATTGCATAGGAAACCTCAACCTCCTTCAGAAAGTTTTCCTAAGTTCCAATAGATTGACATCATCAGTACCGTTGGGTTTATGGGACCTTGAAAATCTCCTGTTCTTAGATCTGTCAATCAATTCTTTTTATGGGAACTTACCTCCAAGCTTGAGCAAGTTGAATGCCATTCAAGAGATAGATTTATCTCAAAACCAATTTACCGGAAACATTCCAAGCACCATAGGCAGTTTGCAAACTCTGAGCTCTCTTAATCTGTCACAAAACTTCTTTACAGGTTCTGTTCCACAAACTTTTGGTGATCAGCTGAAAGGGTTGGAATTGTTGGACTTATCATTCAATAATCTTTCTGGTGCAATACCAAACTCTCTTGAGAAACTCAAATTTCTCAACTACTTAAATCTCTCGTTCAACTACCTCTCCGGAGAGGTCCCTTCTGGGGACGTTTCGTCAACTTCAATGCCCAGTCATTTTTGGAGAATAAAGCACTATGTGGGAGACGAGAGTTTGGACTCCCACCTTGCAAGACTCATAGGACTCAAAAATCAAAAGTATGGAAAAAGAAAGAGAGGCAAGACTCCGAGTTCAGTTGAACAGTTAACAACAGTGGAGCATATTGTGATATCATATCAAGAACTCCGTCGGGCTACAAATGACTTTTGTGAAAGCAACTTACTTGGAGTGGGAAGTTTTGGCTCGGTGTACAAGGGGATACTGTCTAATGGGACAACCATTGCTGTCAAAGTTCTTAAGCTGGAAATGGAGGGTGCTGTAAAAAGTTTTGATGCAGAATGCAAGGTGTGGAGAACAATCCGGCATAGGAATCTTGTAAAGGTCATAACTACATGTTCTAGCCCTGAGGTCAGTGCTTTGGTGCTGCAGTACATGTCTAATGGCAGTCTTGAGAAATGGTTATACTCTCACAACTACTGTCTCAGTATTCTGCAAAGAGTGAGCATGTTGATCGATATTGCATCTGCTTTGGAATATCTCCATCATGGCCAAGCAGAAGCAGTGGTGCACTGTGATGTGAAGCCTGGGAATATCCTTCTTGATGAAGACATGGTAGCACATGTTGCAGACTTCGGTCTTGCAAAAAATTTAGCAAAAAACAAAGAGGAAACACAGACAAGAACCATCGGCACTCTGGGCTACGTAGCACCAGAGTATGGTTCTGCCGGAAAAGTGTCCAAAAAGGGTGATGTCTATAGCTTTGGGATAATGCTGCTGGAAATAATGGCGAGAAAGAAACCCACTGATGAAATGTTTGCCGGAGAAGTTACTTTGAGGCAATGGATAAATGTCTCTATCCCTGATAATGTTCTGGAAGTGGTGGATGCTGGCTTATTGAGCATAGAAGAGGGAAGAGACATGAATGCCACAGAAAGTATCCTAGTGTCCATCCTCGAAATAGGCTTGACGTGTTCGGAAGAAGTAGCAGAGGATAGAATGGACATTAAGGATGTGGTGCCCAAGCTCAAGAAAATAAAATTGGCACTACAACATTGA

Protein Analysis

1086

Amino Acids

119.54

Weight (kDa)

6.96

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 82 - 180 3.7e-07 Leucine-rich repeat region
LRR_14 PF23598 263 - 373 4.6e-06 Leucine-rich repeat region
LRR_8 PF13855 271 - 330 7.4e-07 Leucine rich repeat
LRR_14 PF23598 503 - 649 2.1e-10 Leucine-rich repeat region
LRR_14 PF23598 611 - 723 2.5e-06 Leucine-rich repeat region
LRR_8 PF13855 664 - 724 1.1e-07 Leucine rich repeat
Pkinase PF00069 807 - 1004 9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 808 - 1076 1.5e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 74, 1451, 3220
AccB7I CCANNNNNTGG 2 cut(s) 290, 2921
AccIII TCCGGA 1 cut(s) 2174
AciI CCGC 2 cut(s) 486, 1065
AclWI GGATC 3 cut(s) 327, 957, 1355
AcoI YGGCCR 3 cut(s) 487, 781, 2752
AcsI RAATTY 6 cut(s) 855, 1237, 1300, 1474, 2135, 2851
AcuI CTGAAG 2 cut(s) 781, 1763
AdeI CACNNNGTG 1 cut(s) 2245
AfaI GTAC 8 cut(s) 387, 808, 925, 1051, 1568, 1821, 2456, 2636
AflII CTTAAG 2 cut(s) 989, 2501
AflIII ACRYGT 6 cut(s) 286, 1216, 2597, 2637, 2824, 3173
AhdI GACNNNNNGTC 1 cut(s) 2200
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 3174
AjnI CCWGG 2 cut(s) 1704, 2788
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 3227, 3259
Alw21I GWGCWC 2 cut(s) 2000, 2775
Alw26I GTCTC 7 cut(s) 214, 269, 316, 2244, 2690, 3061, 3114
Alw44I GTGCAC 1 cut(s) 2771
AlwI GGATC 3 cut(s) 327, 957, 1355
AlwNI CAGNNNCTG 1 cut(s) 2033
Ama87I CYCGRG 2 cut(s) 70, 1337
Aor13HI TCCGGA 1 cut(s) 2174
AoxI GGCC 8 cut(s) 487, 622, 737, 781, 1057, 1405, 1525, 2752
ApaLI GTGCAC 1 cut(s) 2771
ApeKI GCWGC 5 cut(s) 394, 441, 1633, 2629, 2977
ApoI RAATTY 6 cut(s) 855, 1237, 1300, 1474, 2135, 2851
Asp700I GAANNNNTTC 4 cut(s) 1329, 1477, 1546, 1787
AspS9I GGNCC 8 cut(s) 174, 247, 1067, 1286, 1406, 1525, 1837, 2182
AsuC2I CCSGG 1 cut(s) 1365
AsuHPI GGTGA 4 cut(s) 686, 1310, 2063, 2963
AvaI CYCGRG 2 cut(s) 70, 1337
AvaII GGWCC 6 cut(s) 174, 247, 1067, 1286, 1837, 2182
AxyI CCTNAGG 1 cut(s) 2610
BaeGI GKGCMC 3 cut(s) 77, 2775, 3225
BalI TGGCCA 2 cut(s) 783, 2754
BanI GGYRCC 3 cut(s) 74, 1451, 3220
BanII GRGCYC 1 cut(s) 2000
BauI CACGAG 1 cut(s) 1005
BbsI GAAGAC 2 cut(s) 654, 2817
Bbv12I GWGCWC 2 cut(s) 2000, 2775
BbvI GCAGC 5 cut(s) 381, 428, 1620, 2616, 2964
BccI CCATC 4 cut(s) 1231, 2754, 2897, 3161
BceAI ACGGC 1 cut(s) 637
BcgI CGANNNNNNTGC 2 cut(s) 2698, 2732
BciT130I CCWGG 2 cut(s) 1706, 2790
BciVI GTATCC 2 cut(s) 2457, 3152
BclI TGATCA 1 cut(s) 2053
BcnI CCSGG 1 cut(s) 1365
BcoDI GTCTC 7 cut(s) 214, 269, 316, 2244, 2690, 3061, 3114
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 7 cut(s) 35, 741, 936, 1026, 1173, 2604, 3146
BfmI CTRYAG 3 cut(s) 1158, 2630, 2959
BfrI CTTAAG 2 cut(s) 989, 2501
BfuI GTATCC 2 cut(s) 2457, 3152
BglII AGATCT 1 cut(s) 1861
BisI GCNGC 6 cut(s) 395, 442, 487, 1634, 2630, 2978
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 6 cut(s) 396, 443, 488, 1635, 2631, 2979
Bme1390I CCNGG 3 cut(s) 1365, 1706, 2790
Bme18I GGWCC 6 cut(s) 174, 247, 1067, 1286, 1837, 2182
BmeRI GACNNNNNGTC 1 cut(s) 2200
BmeT110I CYCGRG 2 cut(s) 70, 1337
BmgBI CACGTC 1 cut(s) 3174
BmgT120I GGNCC 8 cut(s) 174, 247, 1067, 1286, 1406, 1525, 1837, 2182
BmiI GGNNCC 7 cut(s) 76, 347, 1453, 1703, 1838, 2184, 3222
BmrFI CCNGG 3 cut(s) 1365, 1706, 2790
BmrI ACTGGG 1 cut(s) 2211
BmsI GCATC 3 cut(s) 2527, 2735, 3079
BmuI ACTGGG 1 cut(s) 2211
BoxI GACNNNNGTC 1 cut(s) 2838
BpiI GAAGAC 2 cut(s) 654, 2817
BpmI CTGGAG 1 cut(s) 1475
Bpu10I CCTNAGC 1 cut(s) 1061
Bpu1102I GCTNAGC 1 cut(s) 482
BpuEI CTTGAG 7 cut(s) 545, 908, 979, 1924, 2144, 2675, 3215
BpuMI CCSGG 1 cut(s) 1365
Bsa29I ATCGAT 1 cut(s) 2718
BsaAI YACGTR 2 cut(s) 1255, 2908
BsaBI GATNNNNATC 1 cut(s) 1810
BsaI GGTCTC 3 cut(s) 214, 269, 316
BsaJI CCNNGG 5 cut(s) 243, 268, 1536, 1705, 2789
BsaWI WCCGGW 3 cut(s) 1211, 1955, 2174
BsaXI ACNNNNNCTCC 4 cut(s) 916, 946, 2156, 2186
Bse1I ACTGG 6 cut(s) 406, 634, 925, 1070, 1492, 2217
Bse21I CCTNAGG 1 cut(s) 2610
Bse3DI GCAATG 2 cut(s) 2484, 3050
Bse8I GATNNNNATC 1 cut(s) 1810
BseAI TCCGGA 1 cut(s) 2174
BseBI CCWGG 2 cut(s) 1706, 2790
BseCI ATCGAT 1 cut(s) 2718
BseDI CCNNGG 5 cut(s) 243, 268, 1536, 1705, 2789
BseGI GGATG 5 cut(s) 1223, 1268, 3094, 3153, 3220
BseJI GATNNNNATC 1 cut(s) 1810
BseMI GCAATG 2 cut(s) 2484, 3050
BseMII CTCAG 5 cut(s) 1425, 1583, 1985, 2601, 2701
BseNI ACTGG 6 cut(s) 406, 634, 925, 1070, 1492, 2217
BseRI GAGGAG 4 cut(s) 70, 1087, 1144, 1476
BseSI GKGCMC 3 cut(s) 77, 2775, 3225
BseXI GCAGC 5 cut(s) 381, 428, 1620, 2616, 2964
BshFI GGCC 8 cut(s) 489, 624, 739, 783, 1059, 1407, 1527, 2754
BshNI GGYRCC 3 cut(s) 74, 1451, 3220
BshVI ATCGAT 1 cut(s) 2718
BsiHKAI GWGCWC 2 cut(s) 2000, 2775
BsiHKCI CYCGRG 2 cut(s) 70, 1337
BsiSI CCGG 7 cut(s) 1212, 1365, 1956, 2175, 2566, 2931, 3024
BsiWI CGTACG 1 cut(s) 1049
BslFI GGGAC 5 cut(s) 1205, 1850, 2168, 2207, 2491
BsmAI GTCTC 7 cut(s) 214, 269, 316, 2244, 2690, 3061, 3114
BsmBI CGTCTC 1 cut(s) 2244
BsmFI GGGAC 5 cut(s) 1205, 1850, 2168, 2207, 2491
BsmI GAATGC 4 cut(s) 999, 1921, 2549, 3133
BsnI GGCC 8 cut(s) 489, 624, 739, 783, 1059, 1407, 1527, 2754
Bso31I GGTCTC 3 cut(s) 214, 269, 316
BsoBI CYCGRG 2 cut(s) 70, 1337
Bsp1286I GDGCHC 4 cut(s) 77, 2000, 2775, 3225
Bsp13I TCCGGA 1 cut(s) 2174
Bsp1407I TGTACA 2 cut(s) 806, 2454
Bsp143I GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
Bsp1720I GCTNAGC 1 cut(s) 482
BspACI CCGC 2 cut(s) 486, 1065
BspANI GGCC 8 cut(s) 489, 624, 739, 783, 1059, 1407, 1527, 2754
BspCNI CTCAG 5 cut(s) 1426, 1584, 1986, 2602, 2700
BspDI ATCGAT 1 cut(s) 2718
BspEI TCCGGA 1 cut(s) 2174
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 7 cut(s) 76, 347, 1453, 1703, 1838, 2184, 3222
BspMAI CTGCAG 2 cut(s) 1162, 2634
BspPI GGATC 3 cut(s) 327, 957, 1355
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 3 cut(s) 74, 1451, 3220
BspTI CTTAAG 2 cut(s) 989, 2501
BspTNI GGTCTC 3 cut(s) 214, 269, 316
BsrDI GCAATG 2 cut(s) 2484, 3050
BsrGI TGTACA 2 cut(s) 806, 2454
BsrI ACTGG 6 cut(s) 406, 634, 925, 1070, 1492, 2217
BssECI CCNNGG 5 cut(s) 243, 268, 1536, 1705, 2789
BssMI GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
BssSI CACGAG 1 cut(s) 1005
BssT1I CCWWGG 3 cut(s) 243, 268, 1536
Bst2BI CACGAG 1 cut(s) 1005
Bst2UI CCWGG 2 cut(s) 1706, 2790
Bst4CI ACNGT 8 cut(s) 157, 1661, 1824, 2349, 2359, 2469, 2684, 2777
Bst6I CTCTTC 3 cut(s) 117, 3105, 3112
BstAFI CTTAAG 2 cut(s) 989, 2501
BstAPI GCANNNNNTGC 1 cut(s) 2828
BstAUI TGTACA 2 cut(s) 806, 2454
BstBAI YACGTR 2 cut(s) 1255, 2908
BstC8I GCNNGC 4 cut(s) 58, 399, 785, 3094
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 5 cut(s) 1223, 1268, 3094, 3153, 3220
BstKTI GATC 7 cut(s) 103, 322, 965, 1363, 1864, 2056, 2718
BstMAI GTCTC 7 cut(s) 214, 269, 316, 2244, 2690, 3061, 3114
BstMBI GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
BstMWI GCNNNNNNNGC 5 cut(s) 116, 1977, 2828, 2901, 3102
BstNI CCWGG 2 cut(s) 1706, 2790
BstNSI RCATGY 8 cut(s) 60, 290, 401, 1220, 2601, 2641, 2713, 2828
BstPAI GACNNNNGTC 1 cut(s) 2838
BstSCI CCNGG 3 cut(s) 1363, 1704, 2788
BstSFI CTRYAG 3 cut(s) 1158, 2630, 2959
BstSLI GKGCMC 3 cut(s) 77, 2775, 3225
BstSNI TACGTA 2 cut(s) 1255, 2908
BstV1I GCAGC 5 cut(s) 381, 428, 1620, 2616, 2964
BstV2I GAAGAC 2 cut(s) 654, 2817
BstX2I RGATCY 4 cut(s) 319, 962, 1360, 1861
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 4 cut(s) 319, 962, 1360, 1861
Bsu15I ATCGAT 1 cut(s) 2718
Bsu36I CCTNAGG 1 cut(s) 2610
BsuI GTATCC 2 cut(s) 2457, 3152
BsuRI GGCC 8 cut(s) 489, 624, 739, 783, 1059, 1407, 1527, 2754
BsuTUI ATCGAT 1 cut(s) 2718
BtgZI GCGATG 1 cut(s) 359
BtrI CACGTC 1 cut(s) 3174
BtsCI GGATG 5 cut(s) 1223, 1268, 3094, 3153, 3220
BtsI GCAGTG 2 cut(s) 237, 2772
BtsIMutI CAGTG 8 cut(s) 96, 237, 1077, 2364, 2623, 2772, 2773, 3006
Cac8I GCNNGC 4 cut(s) 58, 399, 785, 3094
CaiI CAGNNNCTG 1 cut(s) 2033
CciI TCATGA 1 cut(s) 712
Cfr13I GGNCC 8 cut(s) 174, 247, 1067, 1286, 1406, 1525, 1837, 2182
ClaI ATCGAT 1 cut(s) 2718
Csp6I GTAC 8 cut(s) 386, 807, 924, 1050, 1567, 1820, 2455, 2635
CspCI CAANNNNNGTGG 4 cut(s) 558, 593, 1576, 1611
CviQI GTAC 8 cut(s) 386, 807, 924, 1050, 1567, 1820, 2455, 2635
DpnI GATC 7 cut(s) 102, 321, 964, 1362, 1863, 2055, 2717
DpnII GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
DraI TTTAAA 1 cut(s) 1446
DraIII CACNNNGTG 1 cut(s) 2245
DriI GACNNNNNGTC 1 cut(s) 2200
EaeI YGGCCR 3 cut(s) 487, 781, 2752
Eam1104I CTCTTC 3 cut(s) 117, 3105, 3112
Eam1105I GACNNNNNGTC 1 cut(s) 2200
EarI CTCTTC 3 cut(s) 117, 3105, 3112
Ecl136II GAGCTC 1 cut(s) 1998
Eco105I TACGTA 2 cut(s) 1255, 2908
Eco130I CCWWGG 3 cut(s) 243, 268, 1536
Eco24I GRGCYC 1 cut(s) 2000
Eco31I GGTCTC 3 cut(s) 214, 269, 316
Eco32I GATATC 1 cut(s) 2376
Eco47I GGWCC 6 cut(s) 174, 247, 1067, 1286, 1837, 2182
Eco53kI GAGCTC 1 cut(s) 1998
Eco57I CTGAAG 2 cut(s) 781, 1763
Eco81I CCTNAGG 1 cut(s) 2610
Eco88I CYCGRG 2 cut(s) 70, 1337
EcoICRI GAGCTC 1 cut(s) 1998
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoO109I RGGNCCY 2 cut(s) 1837, 2182
EcoRII CCWGG 2 cut(s) 1704, 2788
EcoRV GATATC 1 cut(s) 2376
EcoT14I CCWWGG 3 cut(s) 243, 268, 1536
EcoT38I GRGCYC 1 cut(s) 2000
ErhI CCWWGG 3 cut(s) 243, 268, 1536
Esp3I CGTCTC 1 cut(s) 2244
FaqI GGGAC 5 cut(s) 1205, 1850, 2168, 2207, 2491
FbaI TGATCA 1 cut(s) 2053
Fnu4HI GCNGC 6 cut(s) 395, 442, 487, 1634, 2630, 2978
FokI GGATG 5 cut(s) 1210, 1255, 3101, 3140, 3227
FriOI GRGCYC 1 cut(s) 2000
Fsp4HI GCNGC 6 cut(s) 395, 442, 487, 1634, 2630, 2978
FspBI CTAG 7 cut(s) 35, 741, 936, 1026, 1173, 2604, 3146
GluI GCNGC 6 cut(s) 395, 442, 487, 1634, 2630, 2978
GsuI CTGGAG 1 cut(s) 1475
HaeIII GGCC 8 cut(s) 489, 624, 739, 783, 1059, 1407, 1527, 2754
HapII CCGG 7 cut(s) 1212, 1365, 1956, 2175, 2566, 2931, 3024
HincII GTYRAC 2 cut(s) 2205, 2352
HindII GTYRAC 2 cut(s) 2205, 2352
HindIII AAGCTT 5 cut(s) 187, 449, 479, 1558, 1900
HpaI GTTAAC 1 cut(s) 2352
HpaII CCGG 7 cut(s) 1212, 1365, 1956, 2175, 2566, 2931, 3024
HphI GGTGA 4 cut(s) 686, 1310, 2063, 2963
Hpy166II GTNNAC 7 cut(s) 221, 635, 766, 2205, 2352, 2455, 2773
Hpy188I TCNGA 9 cut(s) 326, 800, 1435, 1593, 1741, 1782, 1995, 2334, 3181
Hpy8I GTNNAC 7 cut(s) 221, 635, 766, 2205, 2352, 2455, 2773
Hpy99I CGWCG 1 cut(s) 2397
HpyCH4III ACNGT 8 cut(s) 157, 1661, 1824, 2349, 2359, 2469, 2684, 2777
HpyCH4IV ACGT 5 cut(s) 1254, 1565, 2196, 2907, 3173
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 1977, 2828, 2901, 3102
HpySE526I ACGT 5 cut(s) 1254, 1565, 2196, 2907, 3173
Kpn2I TCCGGA 1 cut(s) 2174
Ksp22I TGATCA 1 cut(s) 2053
KspAI GTTAAC 1 cut(s) 2352
Kzo9I GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 2362
Lsp1109I GCAGC 5 cut(s) 381, 428, 1620, 2616, 2964
LweI GCATC 3 cut(s) 2527, 2735, 3079
MaeI CTAG 7 cut(s) 35, 741, 936, 1026, 1173, 2604, 3146
MaeII ACGT 5 cut(s) 1254, 1565, 2196, 2907, 3173
MalI GATC 7 cut(s) 102, 321, 964, 1362, 1863, 2055, 2717
MboI GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
MfeI CAATTG 3 cut(s) 169, 276, 1614
MflI RGATCY 4 cut(s) 319, 962, 1360, 1861
MhlI GDGCHC 4 cut(s) 77, 2000, 2775, 3225
MlsI TGGCCA 2 cut(s) 783, 2754
MluNI TGGCCA 2 cut(s) 783, 2754
MlyI GAGTC 6 cut(s) 243, 1619, 2258, 2274, 2283, 2323
MmeI TCCRAC 6 cut(s) 114, 390, 452, 1296, 2049, 2058
Mox20I TGGCCA 2 cut(s) 783, 2754
MroI TCCGGA 1 cut(s) 2174
MroXI GAANNNNTTC 4 cut(s) 1329, 1477, 1546, 1787
MscI TGGCCA 2 cut(s) 783, 2754
MslI CAYNNNNRTG 1 cut(s) 2778
Msp20I TGGCCA 2 cut(s) 783, 2754
MspA1I CMGCKG 1 cut(s) 2059
MspCI CTTAAG 2 cut(s) 989, 2501
MspI CCGG 7 cut(s) 1212, 1365, 1956, 2175, 2566, 2931, 3024
MspR9I CCNGG 3 cut(s) 1365, 1706, 2790
MunI CAATTG 3 cut(s) 169, 276, 1614
Mva1269I GAATGC 4 cut(s) 999, 1921, 2549, 3133
MvaI CCWGG 2 cut(s) 1706, 2790
MwoI GCNNNNNNNGC 5 cut(s) 116, 1977, 2828, 2901, 3102
NciI CCSGG 1 cut(s) 1365
NdeII GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
NlaIV GGNNCC 7 cut(s) 76, 347, 1453, 1703, 1838, 2184, 3222
NmuCI GTSAC 2 cut(s) 235, 2010
NspI RCATGY 8 cut(s) 60, 290, 401, 1220, 2601, 2641, 2713, 2828
PaeI GCATGC 2 cut(s) 60, 401
PagI TCATGA 1 cut(s) 712
PciI ACATGT 5 cut(s) 286, 1216, 2597, 2637, 2824
PciSI GCTCTTC 1 cut(s) 117
PctI GAATGC 4 cut(s) 999, 1921, 2549, 3133
PdmI GAANNNNTTC 4 cut(s) 1329, 1477, 1546, 1787
PfeI GAWTC 6 cut(s) 1010, 1045, 1182, 1438, 1742, 2575
Pfl23II CGTACG 1 cut(s) 1049
PflMI CCANNNNNTGG 2 cut(s) 290, 2921
PfoI TCCNGGA 1 cut(s) 1363
PkrI GCNGC 6 cut(s) 396, 443, 488, 1635, 2631, 2979
PleI GAGTC 6 cut(s) 242, 1618, 2258, 2274, 2283, 2323
PpsI GAGTC 6 cut(s) 242, 1618, 2258, 2274, 2283, 2323
Ppu21I YACGTR 2 cut(s) 1255, 2908
PpuMI RGGWCCY 2 cut(s) 1837, 2182
PscI ACATGT 5 cut(s) 286, 1216, 2597, 2637, 2824
PshAI GACNNNNGTC 1 cut(s) 2838
Psp124BI GAGCTC 1 cut(s) 2000
Psp5II RGGWCCY 2 cut(s) 1837, 2182
Psp6I CCWGG 2 cut(s) 1704, 2788
PspGI CCWGG 2 cut(s) 1704, 2788
PspLI CGTACG 1 cut(s) 1049
PspN4I GGNNCC 7 cut(s) 76, 347, 1453, 1703, 1838, 2184, 3222
PspPI GGNCC 8 cut(s) 174, 247, 1067, 1286, 1406, 1525, 1837, 2182
PspPPI RGGWCCY 2 cut(s) 1837, 2182
PsrI GAACNNNNNNTAC 2 cut(s) 1704, 1736
PstI CTGCAG 2 cut(s) 1162, 2634
PstNI CAGNNNCTG 1 cut(s) 2033
PsuI RGATCY 4 cut(s) 319, 962, 1360, 1861
PvuII CAGCTG 1 cut(s) 2059
RsaI GTAC 8 cut(s) 387, 808, 925, 1051, 1568, 1821, 2456, 2636
RsaNI GTAC 8 cut(s) 386, 807, 924, 1050, 1567, 1820, 2455, 2635
RseI CAYNNNNRTG 1 cut(s) 2778
SacI GAGCTC 1 cut(s) 2000
SapI GCTCTTC 1 cut(s) 117
SatI GCNGC 6 cut(s) 395, 442, 487, 1634, 2630, 2978
Sau3AI GATC 7 cut(s) 100, 319, 962, 1360, 1861, 2053, 2715
Sau96I GGNCC 8 cut(s) 174, 247, 1067, 1286, 1406, 1525, 1837, 2182
SchI GAGTC 6 cut(s) 243, 1619, 2258, 2274, 2283, 2323
ScrFI CCNGG 3 cut(s) 1365, 1706, 2790
SduI GDGCHC 4 cut(s) 77, 2000, 2775, 3225
SfaNI GCATC 3 cut(s) 2527, 2735, 3079
SfcI CTRYAG 3 cut(s) 1158, 2630, 2959
SinI GGWCC 6 cut(s) 174, 247, 1067, 1286, 1837, 2182
SmiMI CAYNNNNRTG 1 cut(s) 2778
SmlI CTYRAG 9 cut(s) 524, 887, 958, 989, 1903, 2123, 2501, 2654, 3230
SmoI CTYRAG 9 cut(s) 524, 887, 958, 989, 1903, 2123, 2501, 2654, 3230
SnaBI TACGTA 2 cut(s) 1255, 2908
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 2 cut(s) 60, 401
SsiI CCGC 2 cut(s) 486, 1065
SspI AATATT 1 cut(s) 1086
SspMI CTAG 7 cut(s) 35, 741, 936, 1026, 1173, 2604, 3146
SstI GAGCTC 1 cut(s) 2000
StyD4I CCNGG 3 cut(s) 1363, 1704, 2788
StyI CCWWGG 3 cut(s) 243, 268, 1536
TaaI ACNGT 8 cut(s) 157, 1661, 1824, 2349, 2359, 2469, 2684, 2777
TaiI ACGT 5 cut(s) 1257, 1568, 2199, 2910, 3176
TaqI TCGA 3 cut(s) 1323, 2718, 3159
TaqII GACCGA 1 cut(s) 2828
TatI WGTACW 3 cut(s) 806, 2454, 2634
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 6 cut(s) 1010, 1045, 1182, 1438, 1742, 2575
TscAI CASTG 8 cut(s) 103, 244, 1077, 2364, 2623, 2772, 2780, 3013
TseFI GTSAC 2 cut(s) 235, 2010
TseI GCWGC 5 cut(s) 394, 441, 1633, 2629, 2977
Tsp45I GTSAC 2 cut(s) 235, 2010
TspDTI ATGAA 7 cut(s) 10, 372, 701, 1174, 2823, 3027, 3140
TspGWI ACGGA 2 cut(s) 1299, 2381
TspRI CASTG 8 cut(s) 103, 244, 1077, 2364, 2623, 2772, 2780, 3013
Van91I CCANNNNNTGG 2 cut(s) 290, 2921
Vha464I CTTAAG 2 cut(s) 989, 2501
VneI GTGCAC 1 cut(s) 2771
VpaK11BI GGWCC 6 cut(s) 174, 247, 1067, 1286, 1837, 2182
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 6 cut(s) 855, 1237, 1300, 1474, 2135, 2851
XbaI TCTAGA 1 cut(s) 1025
XceI RCATGY 8 cut(s) 60, 290, 401, 1220, 2601, 2641, 2713, 2828
XcmI CCANNNNNNNNNTGG 2 cut(s) 157, 2896
XmnI GAANNNNTTC 4 cut(s) 1329, 1477, 1546, 1787
XspI CTAG 7 cut(s) 35, 741, 936, 1026, 1173, 2604, 3146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.