RchiOBHm_Chr4g0403311

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000058
Physical Location & Seq
Forward (+)
22406230 .. 22410306
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37507

Sequence Viewer

Length: 3372 bp
ATGCGCACCATGTTCATGGAAAAGCTACAAACTTTCCTTTTTGGAGTAGCGCTACTACACTACTCATGCTTCCTTAAGTTGGCCATATCTTCACCTTCCAACTTCACTGATCAGTCTGCTTTGCTTGCCATTCAATCTCAAATCACTTTTGACCCCACCAATACTGTCTTGGGTGGTGGTAGCTGGATCACCAAAACAAGCTTCTGTAATTGGTTTGGGGTCTCTTGCAGTAAACGCAGGCAAAGAGTCACAGCCTTGGACCTGTCCTATATGAATCTCCAAGGCACCATTTCCCCTCATGTTGGCAATCTCTCCTTCCTAATCTCACTTGATCTCCGCAATAACAGCTTCCTGGGGTTCATACCACATGAAATCAGCCGTTTGCATCGCTTGAGGATACTTGTACTTCAAGGTAACAAGTTGGAAGGAAACATCCCTCCAACTTTACAGCTTTGCCACAAGCTCGAGTCCATATCCCTTTCAATAAACATATTAAGTGGTCTCATACCTAAAGAGTTGGGTTTCCTTCCCAGGGTTCGTAAATTATATCTCGGTGCAAACAATCTCACAGGTGAAATTCCTTCGTCTCTGGGTAACATTTCAACCTTACAAGAATTAAGTCTGGTCGAAAATGGTCTCACAGGTTCATTCCCTTCTGCTCTACTCAACGTATCTACTCTAGCTCTTATTTCCCTATCTGGCAATTACATGTCAGGTAGTCTTCCTGTAGATCTTTGCCGTAATTGGCCTAATACTTATTACATTTCTCTATCTGACAACAAATTCGGTGGCCAACTGACTTTCGGAATAAGCCAATGTAGAGAGCTTGAGATCTTATCTCTGTCATACAATAATTTCGTCGGAAGTATTCCGGGAGAAATTGGGAGTTTACAACATCTTAAAACTCTATTTCTTGGTGTCAATAACCTAACTGGTGCTATACCTCCAACAATAGGTAACCTATCAAACTTGCAGTTCTTTACTGTGCCAGATAATAATTTAGAGGGAAGTATTCCAAGTGATTTGGGGCATCTTTCTAAACTAAATTATCTCTCTTTTGCTCGGAATAGCCTAACAGGTGAAATACCTGAGTCCATTTTCAATATCTCCTCCTTAAATACTTTACAAGTACAAACTAATGCTCTTTCTGGAGAATTTCCTTCTCGAGCTATGATTTCTAACCTCAATACCCTATACTGTTATACCAATCACATTACAGGAAATCTACCATCCTATTTTTCAAATTTAAGCCAGCTTGTTGAGCTTGATGTTTCCAACAACTCACTTTATGGACCAATACCCATGAACCTTGGTAGCTCAAAATACCTCGAATTCCTTAACTTGGGAGGAAATCAGTTGTCAGGGGAACCTGGAGTTCCTGTACTTAGGTTCCTTTCTTCTATATTTAATAGCAGTTCACTGAAACATTTATTTTTGAGTAGAAATCCTCTAGACGGGATCATGCCCGATTCATTTGGAAATTTCTCATATTCCCTCGAGATATTTTTTGCAACTGCCTGCCAGATTAAGGGCCCTATACCCAAGACGATTCAGTTGTTGAAAAATCTGACCTTCCTTGCCTTAAGTGACAACAATATTAGTGGGAAAATACCGTCATCAATTGGAGCATTGGAAATGTTGCAGAGATTGTATCTTGAAAACAACAAGATTGAAGGGCTCATTCCTAATGAGCTTTGTCTTTTGCGGAACCTGGGGGATCTATCACTAGGAAATAATCAAATCTCTGGATCAATCCCAAATTGCATTGCAAATCTCAGTCGCATTCAAAGACTTCTTCTAAGTTCCAATAGATTGACATCTGCAATACCAGTGAATTTATGGAATCTTAAAAATCTTCTGTTCTTAAATTTGTCATCCAATATTTTCAGTGGATACTTACCTCCAAGCATGAAAAGGTCGGTTGCTGTTCAGATGGTCGACTTATCTGACAATCAAGTTACTGGGAACATTTCAAGCATTGTCGGAGCCTTCCCAAGCATAAACTCTCTAAGCTTAGCAAACAACTTGTTTATAGGATCTCTTCCGCAAACTTTGGGTGATCTGAAAGGATTGGAGTTTTTGGACCTATCAAACAATAATCTGTCTGGGCCAATTCCAAAGTCTCTTGAAACACTTAAATATCTGAACTACTTAAATTTATCTTTTAATCAACTCTCTGGAGAGATTCCTTCTAGTGGAGCTTTTATCGACTTCACTGCCCTATCATTTTTGGAGAATAGAGGCCTTTGTGGGAGACCGGAGTTTGGAGTCCCACCTTGCATGGCTCTTGGAACTCAGAAGTCAAAGATGGCAAATCATTTACTCAAGTATGTTCTTCCAGGCATTGCATCAACAATGATCTTGATAGCTCTCATATACATGGTGACAAAGTGTCGAAAAAGAGATGAAGGCATTCCAAGTTCAGTACAAGCGTTAACAACAACAGAGCATAGAATGATGTCATATCATCAACTCCGTCGTGCTACAAATGGCTTCTGTGAAAGCAACTTGCTTGGAGTTGGAAGTTTTGGCTCGGTATACAAGGGGATTTTAACAGATGGGACAATTGTTGCTGTTAAAGTTTTAAATTTGCAAATGGAGGGAGCCTTTAAAAGTTTTGATGCAGAATGTAAAGTTTGGCGGAGAATTCGGCACAGAAATCTTGTCAAGATCATGACTACATGCTCTAACCATGAGGTAAGAGCTTTGGTGCTGCAATACATGTCTAATGGCAGCCTTGAGAAGTGGCTATACTCTCACAACTATTGTTTGACTCTTCTGCAAAGAGTGGCCATGTTGATCGACATTGCACTGGCTTTGGACTATCTCCATCATGGTCAATCAGAAGGTGTGGTGCACTGTGATTTGAAGCCTAGCAATATCCTTCTCGATGAAGACATGGTTGCACATGTTGGAGACTTCGGGCTTGCAAAAATTATAGCAATTAACAAAGATGCAACACAGACTAGAACTCTCGGCACACTCGGCTACGTTGCACCAGAGTACGGTTCTGCAGGAGTGGTGTCCGCAAGAGGTGATATTTATAGTTTTGGGATAATGTTGATGGAAATAATTGTGAGAAAGAAACCCACAGATGAAATGTTTGCTGGAGAACTGACTTTGAGACAATGGATAAATGCTTCGCTTCCTGACAGTGTTCTCGAAGTAGTGGATGCTGGCCTATTGAGCATAGAAGATGAAACTAGAGACATGAATGCCACAGTAAAAATCATTTTGTCAATACTGGAAATAGGTTTGGGATGTTCTGAAGAAGTGGCTCAGGAAAGAATCAACATTAAGGATGTAGTGCCCAAGCTCACGAAAATAAAATGTGCACTAGAAGATAGAAATGGCGGTGTTTATTATCCATGA

Protein Analysis

1123

Amino Acids

123.15

Weight (kDa)

7.5

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 86 - 216 2.4e-07 Leucine-rich repeat region
LRR_8 PF13855 274 - 334 8.7e-06 Leucine rich repeat
LRR_14 PF23598 340 - 427 4.7e-07 Leucine-rich repeat region
LRR_14 PF23598 515 - 625 3.7e-09 Leucine-rich repeat region
LRR_8 PF13855 665 - 725 7.4e-07 Leucine rich repeat
Pkinase PF00069 835 - 1032 2.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 836 - 1034 7.8e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 2 cut(s) 1938, 2538
AciI CCGC 6 cut(s) 337, 1705, 2045, 2641, 3027, 3354
AclWI GGATC 5 cut(s) 194, 1466, 1725, 1756, 2044
AcoI YGGCCR 3 cut(s) 81, 790, 2790
AcuI CTGAAG 1 cut(s) 3288
AfaI GTAC 5 cut(s) 405, 1131, 1383, 2427, 3005
AfeI AGCGCT 1 cut(s) 51
AflII CTTAAG 2 cut(s) 74, 1582
AflIII ACRYGT 3 cut(s) 708, 2721, 2908
AhdI GACNNNNNGTC 1 cut(s) 2391
AjnI CCWGG 5 cut(s) 351, 530, 1369, 1710, 2338
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
Alw21I GWGCWC 2 cut(s) 2859, 3337
Alw26I GTCTC 9 cut(s) 226, 506, 591, 641, 2127, 2248, 2910, 3118, 3201
Alw44I GTGCAC 2 cut(s) 2855, 3333
AlwI GGATC 5 cut(s) 194, 1466, 1725, 1756, 2044
Ama87I CYCGRG 3 cut(s) 464, 1164, 1496
Aor51HI AGCGCT 1 cut(s) 51
AoxI GGCC 8 cut(s) 81, 746, 790, 1531, 2108, 2242, 2790, 3178
ApaI GGGCCC 1 cut(s) 1535
ApaLI GTGCAC 2 cut(s) 2855, 3333
ApeKI GCWGC 2 cut(s) 2713, 2733
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
Asp700I GAANNNNTTC 1 cut(s) 2412
AspLEI GCGC 2 cut(s) 6, 52
AspS9I GGNCC 6 cut(s) 259, 1292, 1531, 1532, 2083, 2108
AsuC2I CCSGG 1 cut(s) 873
AsuHPI GGTGA 7 cut(s) 84, 181, 584, 1091, 2069, 2395, 3047
AvaI CYCGRG 3 cut(s) 464, 1164, 1496
AvaII GGWCC 3 cut(s) 259, 1292, 2083
BaeGI GKGCMC 4 cut(s) 1535, 2859, 3312, 3337
BalI TGGCCA 3 cut(s) 83, 792, 2792
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 2 cut(s) 1535, 1680
BarI GAAGNNNNNNTAC 2 cut(s) 2735, 2767
BbsI GAAGAC 2 cut(s) 713, 2901
Bbv12I GWGCWC 2 cut(s) 2859, 3337
BbvI GCAGC 2 cut(s) 2700, 2745
BccI CCATC 6 cut(s) 1237, 1927, 2302, 2552, 2838, 3058
BceAI ACGGC 2 cut(s) 363, 723
BcgI CGANNNNNNTGC 2 cut(s) 2198, 2232
BciT130I CCWGG 5 cut(s) 353, 532, 1371, 1712, 2340
BciVI GTATCC 2 cut(s) 390, 1886
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 873
BcoDI GTCTC 9 cut(s) 226, 506, 591, 641, 2127, 2248, 2910, 3118, 3201
BfaI CTAG 8 cut(s) 680, 1451, 1727, 2193, 2874, 2967, 3204, 3338
BfmI CTRYAG 2 cut(s) 726, 3012
BfoI RGCGCY 1 cut(s) 53
BfrI CTTAAG 2 cut(s) 74, 1582
BfuI GTATCC 2 cut(s) 390, 1886
BglII AGATCT 2 cut(s) 730, 831
BisI GCNGC 2 cut(s) 2714, 2734
BlpI GCTNAGC 1 cut(s) 2014
BlsI GCNGC 2 cut(s) 2715, 2735
Bme1390I CCNGG 6 cut(s) 353, 532, 873, 1371, 1712, 2340
Bme18I GGWCC 3 cut(s) 259, 1292, 2083
BmeRI GACNNNNNGTC 1 cut(s) 2391
BmeT110I CYCGRG 3 cut(s) 464, 1164, 1496
BmgT120I GGNCC 6 cut(s) 259, 1292, 1531, 1532, 2083, 2108
BmiI GGNNCC 7 cut(s) 286, 1368, 1391, 1533, 1709, 1987, 2605
BmrFI CCNGG 6 cut(s) 353, 532, 873, 1371, 1712, 2340
BmrI ACTGGG 1 cut(s) 1971
BmsI GCATC 6 cut(s) 394, 1039, 2357, 2611, 2944, 3163
BmuI ACTGGG 1 cut(s) 1971
BpiI GAAGAC 2 cut(s) 713, 2901
BpmI CTGGAG 4 cut(s) 1170, 1392, 2199, 3129
Bpu10I CCTNAGC 1 cut(s) 3279
Bpu1102I GCTNAGC 1 cut(s) 2014
BpuEI CTTGAG 4 cut(s) 412, 848, 2309, 2759
BpuMI CCSGG 1 cut(s) 873
BsaBI GATNNNNATC 1 cut(s) 1816
BsaI GGTCTC 4 cut(s) 226, 506, 641, 2248
BsaJI CCNNGG 7 cut(s) 255, 280, 352, 530, 531, 1309, 1711
BsaWI WCCGGW 1 cut(s) 2257
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 1365, 1395
Bse1I ACTGG 5 cut(s) 937, 1829, 1966, 2817, 3249
Bse3DI GCAATG 3 cut(s) 1764, 2343, 2805
Bse8I GATNNNNATC 1 cut(s) 1816
BseBI CCWGG 5 cut(s) 353, 532, 1371, 1712, 2340
BseDI CCNNGG 7 cut(s) 255, 280, 352, 530, 531, 1309, 1711
BseGI GGATG 6 cut(s) 432, 1229, 1874, 3178, 3266, 3307
BseJI GATNNNNATC 1 cut(s) 1816
BseMI GCAATG 3 cut(s) 1764, 2343, 2805
BseMII CTCAG 4 cut(s) 1080, 1789, 2309, 3293
BseNI ACTGG 5 cut(s) 937, 1829, 1966, 2817, 3249
BseRI GAGGAG 1 cut(s) 1099
BseSI GKGCMC 4 cut(s) 1535, 2859, 3312, 3337
BseXI GCAGC 2 cut(s) 2700, 2745
BshFI GGCC 8 cut(s) 83, 748, 792, 1533, 2110, 2244, 2792, 3180
BshNI GGYRCC 1 cut(s) 284
BsiHKAI GWGCWC 2 cut(s) 2859, 3337
BsiHKCI CYCGRG 3 cut(s) 464, 1164, 1496
BsiSI CCGG 2 cut(s) 872, 2258
BslFI GGGAC 2 cut(s) 2255, 2575
BsmAI GTCTC 9 cut(s) 226, 506, 591, 641, 2127, 2248, 2910, 3118, 3201
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 2 cut(s) 2255, 2575
BsmI GAATGC 3 cut(s) 1782, 2412, 3220
BsnI GGCC 8 cut(s) 83, 748, 792, 1533, 2110, 2244, 2792, 3180
Bso31I GGTCTC 4 cut(s) 226, 506, 641, 2248
BsoBI CYCGRG 3 cut(s) 464, 1164, 1496
Bsp120I GGGCCC 1 cut(s) 1531
Bsp1286I GDGCHC 5 cut(s) 1535, 1680, 2859, 3312, 3337
Bsp1720I GCTNAGC 1 cut(s) 2014
BspACI CCGC 6 cut(s) 337, 1705, 2045, 2641, 3027, 3354
BspANI GGCC 8 cut(s) 83, 748, 792, 1533, 2110, 2244, 2792, 3180
BspCNI CTCAG 4 cut(s) 1081, 1788, 2308, 3292
BspHI TCATGA 1 cut(s) 2673
BspLI GGNNCC 7 cut(s) 286, 1368, 1391, 1533, 1709, 1987, 2605
BspMAI CTGCAG 1 cut(s) 3016
BspPI GGATC 5 cut(s) 194, 1466, 1725, 1756, 2044
BspT107I GGYRCC 1 cut(s) 284
BspTI CTTAAG 2 cut(s) 74, 1582
BspTNI GGTCTC 4 cut(s) 226, 506, 641, 2248
BsrDI GCAATG 3 cut(s) 1764, 2343, 2805
BsrI ACTGG 5 cut(s) 937, 1829, 1966, 2817, 3249
BssECI CCNNGG 7 cut(s) 255, 280, 352, 530, 531, 1309, 1711
BssNAI GTATAC 1 cut(s) 2539
BssT1I CCWWGG 3 cut(s) 255, 280, 1309
Bst1107I GTATAC 1 cut(s) 2539
Bst2UI CCWGG 5 cut(s) 353, 532, 1371, 1712, 2340
Bst4CI ACNGT 8 cut(s) 166, 985, 1199, 1614, 2861, 3008, 3155, 3223
Bst6I CTCTTC 2 cut(s) 2046, 2781
BstAFI CTTAAG 2 cut(s) 74, 1582
BstC8I GCNNGC 6 cut(s) 126, 239, 1253, 1519, 2928, 3178
BstDEI CTNAG 8 cut(s) 1089, 1385, 1775, 1799, 2009, 2014, 2295, 3279
BstEII GGTNACC 1 cut(s) 956
BstF5I GGATG 6 cut(s) 432, 1229, 1874, 3178, 3266, 3307
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 6, 52
BstMAI GTCTC 9 cut(s) 226, 506, 591, 641, 2127, 2248, 2910, 3118, 3201
BstMWI GCNNNNNNNGC 6 cut(s) 125, 234, 345, 1261, 2985, 3186
BstNI CCWGG 5 cut(s) 353, 532, 1371, 1712, 2340
BstNSI RCATGY 4 cut(s) 712, 2685, 2725, 2912
BstPI GGTNACC 1 cut(s) 956
BstSCI CCNGG 6 cut(s) 351, 530, 871, 1369, 1710, 2338
BstSFI CTRYAG 2 cut(s) 726, 3012
BstSLI GKGCMC 4 cut(s) 1535, 2859, 3312, 3337
BstV1I GCAGC 2 cut(s) 2700, 2745
BstV2I GAAGAC 2 cut(s) 713, 2901
BstX2I RGATCY 4 cut(s) 730, 831, 1717, 2036
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 4 cut(s) 730, 831, 1717, 2036
BstZ17I GTATAC 1 cut(s) 2539
BsuI GTATCC 2 cut(s) 390, 1886
BsuRI GGCC 8 cut(s) 83, 748, 792, 1533, 2110, 2244, 2792, 3180
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 6 cut(s) 432, 1229, 1874, 3178, 3266, 3307
BtsI GCAGTG 1 cut(s) 2214
BtsIMutI CAGTG 8 cut(s) 105, 1418, 1836, 1894, 2214, 2810, 2857, 3160
Cac8I GCNNGC 6 cut(s) 126, 239, 1253, 1519, 2928, 3178
CciI TCATGA 1 cut(s) 2673
CfoI GCGC 2 cut(s) 6, 52
Cfr13I GGNCC 6 cut(s) 259, 1292, 1531, 1532, 2083, 2108
Csp6I GTAC 5 cut(s) 404, 1130, 1382, 2426, 3004
CspCI CAANNNNNGTGG 4 cut(s) 769, 804, 1582, 1617
CviQI GTAC 5 cut(s) 404, 1130, 1382, 2426, 3004
DdeI CTNAG 8 cut(s) 1089, 1385, 1775, 1799, 2009, 2014, 2295, 3279
DraI TTTAAA 2 cut(s) 2586, 2611
DriI GACNNNNNGTC 1 cut(s) 2391
EaeI YGGCCR 3 cut(s) 81, 790, 2790
Eam1104I CTCTTC 2 cut(s) 2046, 2781
Eam1105I GACNNNNNGTC 1 cut(s) 2391
EarI CTCTTC 2 cut(s) 2046, 2781
EciI GGCGGA 1 cut(s) 2656
Eco130I CCWWGG 3 cut(s) 255, 280, 1309
Eco147I AGGCCT 1 cut(s) 2244
Eco24I GRGCYC 2 cut(s) 1535, 1680
Eco31I GGTCTC 4 cut(s) 226, 506, 641, 2248
Eco47I GGWCC 3 cut(s) 259, 1292, 2083
Eco47III AGCGCT 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 3288
Eco88I CYCGRG 3 cut(s) 464, 1164, 1496
Eco91I GGTNACC 1 cut(s) 956
EcoO109I RGGNCCY 2 cut(s) 1531, 1532
EcoO65I GGTNACC 1 cut(s) 956
EcoRI GAATTC 2 cut(s) 1331, 2646
EcoRII CCWGG 5 cut(s) 351, 530, 1369, 1710, 2338
EcoT14I CCWWGG 3 cut(s) 255, 280, 1309
EcoT38I GRGCYC 2 cut(s) 1535, 1680
ErhI CCWWGG 3 cut(s) 255, 280, 1309
Esp3I CGTCTC 1 cut(s) 591
FaqI GGGAC 2 cut(s) 2255, 2575
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 1938, 2538
Fnu4HI GCNGC 2 cut(s) 2714, 2734
FokI GGATG 6 cut(s) 419, 1216, 1861, 3185, 3273, 3314
FriOI GRGCYC 2 cut(s) 1535, 1680
Fsp4HI GCNGC 2 cut(s) 2714, 2734
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 8 cut(s) 680, 1451, 1727, 2193, 2874, 2967, 3204, 3338
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 51
GluI GCNGC 2 cut(s) 2714, 2734
GsuI CTGGAG 4 cut(s) 1170, 1392, 2199, 3129
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 8 cut(s) 83, 748, 792, 1533, 2110, 2244, 2792, 3180
HapII CCGG 2 cut(s) 872, 2258
HhaI GCGC 2 cut(s) 6, 52
Hin6I GCGC 2 cut(s) 4, 50
HinP1I GCGC 2 cut(s) 4, 50
HincII GTYRAC 2 cut(s) 1939, 2436
HindII GTYRAC 2 cut(s) 1939, 2436
HindIII AAGCTT 2 cut(s) 199, 2011
HpaI GTTAAC 1 cut(s) 2436
HpaII CCGG 2 cut(s) 872, 2258
HphI GGTGA 7 cut(s) 84, 181, 584, 1091, 2069, 2395, 3047
Hpy166II GTNNAC 8 cut(s) 233, 890, 1418, 1939, 2436, 2539, 2857, 3335
Hpy8I GTNNAC 8 cut(s) 233, 890, 1418, 1939, 2436, 2539, 2857, 3335
Hpy99I CGWCG 2 cut(s) 863, 2481
HpyCH4III ACNGT 8 cut(s) 166, 985, 1199, 1614, 2861, 3008, 3155, 3223
HpyCH4IV ACGT 2 cut(s) 669, 2991
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 234, 345, 1261, 2985, 3186
HpyF3I CTNAG 8 cut(s) 1089, 1385, 1775, 1799, 2009, 2014, 2295, 3279
HpySE526I ACGT 2 cut(s) 669, 2991
HspAI GCGC 2 cut(s) 4, 50
Ksp22I TGATCA 1 cut(s) 109
KspAI GTTAAC 1 cut(s) 2436
LmnI GCTCC 4 cut(s) 1625, 1985, 2198, 2603
Lsp1109I GCAGC 2 cut(s) 2700, 2745
LweI GCATC 6 cut(s) 394, 1039, 2357, 2611, 2944, 3163
MaeI CTAG 8 cut(s) 680, 1451, 1727, 2193, 2874, 2967, 3204, 3338
MaeII ACGT 2 cut(s) 669, 2991
MaeIII GTNAC 7 cut(s) 247, 413, 593, 956, 1586, 1957, 2383
MfeI CAATTG 2 cut(s) 1620, 2565
MflI RGATCY 4 cut(s) 730, 831, 1717, 2036
MhlI GDGCHC 5 cut(s) 1535, 1680, 2859, 3312, 3337
MlsI TGGCCA 3 cut(s) 83, 792, 2792
MluNI TGGCCA 3 cut(s) 83, 792, 2792
MlyI GAGTC 5 cut(s) 255, 476, 1100, 2277, 2767
MmeI TCCRAC 9 cut(s) 123, 402, 464, 841, 971, 1299, 1963, 2500, 2893
Mox20I TGGCCA 3 cut(s) 83, 792, 2792
MroXI GAANNNNTTC 1 cut(s) 2412
MscI TGGCCA 3 cut(s) 83, 792, 2792
MslI CAYNNNNRTG 2 cut(s) 14, 2378
Msp20I TGGCCA 3 cut(s) 83, 792, 2792
MspCI CTTAAG 2 cut(s) 74, 1582
MspI CCGG 2 cut(s) 872, 2258
MspR9I CCNGG 6 cut(s) 353, 532, 873, 1371, 1712, 2340
MunI CAATTG 2 cut(s) 1620, 2565
Mva1269I GAATGC 3 cut(s) 1782, 2412, 3220
MvaI CCWGG 5 cut(s) 353, 532, 1371, 1712, 2340
MwoI GCNNNNNNNGC 6 cut(s) 125, 234, 345, 1261, 2985, 3186
NciI CCSGG 1 cut(s) 873
NlaIV GGNNCC 7 cut(s) 286, 1368, 1391, 1533, 1709, 1987, 2605
NmeAIII GCCGAG 2 cut(s) 2955, 2964
NmuCI GTSAC 3 cut(s) 247, 1586, 2383
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 4 cut(s) 712, 2685, 2725, 2912
PaeR7I CTCGAG 3 cut(s) 464, 1164, 1496
PagI TCATGA 1 cut(s) 2673
PasI CCCWGGG 1 cut(s) 531
PceI AGGCCT 1 cut(s) 2244
PciI ACATGT 3 cut(s) 708, 2721, 2908
PctI GAATGC 3 cut(s) 1782, 2412, 3220
PdmI GAANNNNTTC 1 cut(s) 2412
PfeI GAWTC 6 cut(s) 274, 1469, 1549, 1843, 2185, 3288
PfoI TCCNGGA 1 cut(s) 871
PkrI GCNGC 2 cut(s) 2715, 2735
PleI GAGTC 5 cut(s) 254, 475, 1099, 2276, 2767
PpsI GAGTC 5 cut(s) 254, 475, 1099, 2276, 2767
PscI ACATGT 3 cut(s) 708, 2721, 2908
Psp6I CCWGG 5 cut(s) 351, 530, 1369, 1710, 2338
PspEI GGTNACC 1 cut(s) 956
PspGI CCWGG 5 cut(s) 351, 530, 1369, 1710, 2338
PspN4I GGNNCC 7 cut(s) 286, 1368, 1391, 1533, 1709, 1987, 2605
PspOMI GGGCCC 1 cut(s) 1531
PspPI GGNCC 6 cut(s) 259, 1292, 1531, 1532, 2083, 2108
PspXI VCTCGAGB 1 cut(s) 464
PstI CTGCAG 1 cut(s) 3016
PsuI RGATCY 4 cut(s) 730, 831, 1717, 2036
RsaI GTAC 5 cut(s) 405, 1131, 1383, 2427, 3005
RsaNI GTAC 5 cut(s) 404, 1130, 1382, 2426, 3004
RseI CAYNNNNRTG 2 cut(s) 14, 2378
SalI GTCGAC 1 cut(s) 1937
SatI GCNGC 2 cut(s) 2714, 2734
Sau96I GGNCC 6 cut(s) 259, 1292, 1531, 1532, 2083, 2108
SchI GAGTC 5 cut(s) 255, 476, 1100, 2277, 2767
ScrFI CCNGG 6 cut(s) 353, 532, 873, 1371, 1712, 2340
SduI GDGCHC 5 cut(s) 1535, 1680, 2859, 3312, 3337
SfaNI GCATC 6 cut(s) 394, 1039, 2357, 2611, 2944, 3163
SfcI CTRYAG 2 cut(s) 726, 3012
Sfr274I CTCGAG 3 cut(s) 464, 1164, 1496
SinI GGWCC 3 cut(s) 259, 1292, 2083
SlaI CTCGAG 3 cut(s) 464, 1164, 1496
SmiMI CAYNNNNRTG 2 cut(s) 14, 2378
SmlI CTYRAG 9 cut(s) 74, 391, 464, 827, 1164, 1496, 1582, 2324, 2738
SmoI CTYRAG 9 cut(s) 74, 391, 464, 827, 1164, 1496, 1582, 2324, 2738
SseBI AGGCCT 1 cut(s) 2244
SsiI CCGC 6 cut(s) 337, 1705, 2045, 2641, 3027, 3354
SspI AATATT 2 cut(s) 1597, 1882
SspMI CTAG 8 cut(s) 680, 1451, 1727, 2193, 2874, 2967, 3204, 3338
StuI AGGCCT 1 cut(s) 2244
StyD4I CCNGG 6 cut(s) 351, 530, 871, 1369, 1710, 2338
StyI CCWWGG 3 cut(s) 255, 280, 1309
TaaI ACNGT 8 cut(s) 166, 985, 1199, 1614, 2861, 3008, 3155, 3223
TaiI ACGT 2 cut(s) 672, 2994
TatI WGTACW 4 cut(s) 403, 1129, 1381, 2425
TfiI GAWTC 6 cut(s) 274, 1469, 1549, 1843, 2185, 3288
TscAI CASTG 8 cut(s) 112, 1425, 1836, 1894, 2221, 2817, 2864, 3160
TseFI GTSAC 3 cut(s) 247, 1586, 2383
TseI GCWGC 2 cut(s) 2713, 2733
Tsp45I GTSAC 3 cut(s) 247, 1586, 2383
TspGWI ACGGA 1 cut(s) 2465
TspRI CASTG 8 cut(s) 112, 1425, 1836, 1894, 2221, 2817, 2864, 3160
Vha464I CTTAAG 2 cut(s) 74, 1582
VneI GTGCAC 2 cut(s) 2855, 3333
VpaK11BI GGWCC 3 cut(s) 259, 1292, 2083
XbaI TCTAGA 1 cut(s) 1450
XceI RCATGY 4 cut(s) 712, 2685, 2725, 2912
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 1836
XhoI CTCGAG 3 cut(s) 464, 1164, 1496
XmiI GTMKAC 2 cut(s) 1938, 2538
XmnI GAANNNNTTC 1 cut(s) 2412
XspI CTAG 8 cut(s) 680, 1451, 1727, 2193, 2874, 2967, 3204, 3338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.