RchiOBHm_Chr4g0402601

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000058
Physical Location & Seq
Reverse (-)
21241064 .. 21244643
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37438

Sequence Viewer

Length: 3036 bp
ATGTTAAGGCACATATTAACAATGGTAACAAGTCGATTTTTCCTCTTGCAAACAAGGTTGTTGCTGGTATACTACTGTTGTATTGCTAGCTTTGCTATAGTGGTAGCAGTACAAACCAGCGATGTCTCCACAGATGAGTCTGCTCTTCTTTCTCTCAAAGCCAGCATCACCATTGAAGGTCAAAACATGATCTTCACCGACTGGTCCACCAACAACATTTGCAACTGGGCTGGGGTTACTTGTGGTGCAAGCCACCGTAGAGTCGAGGTCTTGGACCTGTCTTACTTTGGTCTTTCAGGCACCATTCCTGCGGAGCTAGGTAACCTGTCATTTCTTGTTGATCTGGACTTCACAAATAACAGCTTCCAAGGTCCCTTGCCCCAAGAATTGTCTCGTCTACACAGGTTGAAGTTCCTTAGCTTTGGACTCAACAACTTTGGGGGAACAATTCCATCATGGTTTGGGTCCTTGTCCAAACTTCAAACCTTGGATTTGTACGGTAATCAATTTTCAGGTTCCATGCCCGCTGCCATCTTCAACTTATCTGCACTACAAGTAATAGATCTCAAAAATAATAATCTATCGGGTACTATACCACATGGAGTAGGTGATCTTCCAAATCTACAGATTTTGTCACTCATGGGTAATAATCTTAATGGCCTCGTCCCATCCACAATCTTCAACATGTCCACAATAAGAGTGATATCTATTAGTAGTAATAATCTCTCAGGTAGCTTCCCAACAAACATAGGCCTTGGATTTCCAAACATACAAGAGTTTTATGCATCGAGTAATAAACTCAGTGGAGTAATTCCCAAATCCATCTCGAATGCTTCTAAGCTCACTGGCCTAGACCTGGGAGGTAACTCATTTTATGGCTCTATACCTAGCTCGCTTTGTGCCTCACCAAACCTTGAGCGGCTGGTCTTGGAATCGAACAACTTGACGATTGACAAATTTACTCCAGAAGTAAATATACTCTCTTGCTTAGCCACTCTTACAAAGTTGAGAAAATTATGGCTGTCTGACAATCCACTAAATGCCATGCTTCCATTTTCTCTTGGAAATCTCTCTACATCACTTGAATATATGATATTAAGGAATTGCAACATGTCGGGTAAGATTCCGAATGATATTGGCAACTTGAGCAGCTTGATCTTCTTAGACTTCGGATTGAATGAATTGAGTGGATCAATTCCAACTACCATAGGAAGACTACAAAAACTTCAAGTTTTTAACTTGAGTAACAACATATTGCAAGGATATGTTCCAGATGAACTTTGTCAGCTGAAAAACCTAGGTGACTTGCTTCTGCAAGGCAATCAACTCTCTGGTTCTATACCTTCCTGCTTAGGTGATCTAAATGCATCTCTAAGAATTCTATCACTAGGGTCCAATTTGTTAACTTCAACAATACCATCTACCTTGTGGAGGCTTACTTATATCCTACACTTGGACTTGTCATCCAATTCTCTAAGTGGACTTCTCTCAGAAGAGCTTGGTAATTTGAAAGTTGTGACAGATATATATTTATCAAACAACCATTTTTCGGGTTCGATACCAATTAGCATTGGATTTCGCACAGATCTTGTCATTCTCAACCTAGCAAACAATAGTTTAGAAGGCTCTATTCCTGATACTTTTATTGGATGTCCAAGCTTAGAATTCTTGGATTTATCCAAAAACAATCTGTCAGGGGTGATTCCAAAGTCTCTACAATCACTTTTATATCTCAGATATTTGAATTTGTCCATCAACAGACTCCATGGAGAAATTCCAGAAGGTGGAGCTTTCGAACACTTGTCTTATGAATCAATCTTCTCGAATGGTGAACTCTGTGGTTCACCCCACCTGCAGGTTCCACTGTGTCCAAGTTCAACGAGAAGTACTCCACTGTGTCAAAATAGATGCATTACTATTTTGAAGTGTATTATTCCAGGGATCCCCTTAGCAATACTCATAGTGGCCTTCATATGGAGATTGATACTGCATAGAAAAAGGAATGCAAAAGCTGCAAAAGTGACTACATTGTTACCACCACAACCTCTTTGGAGAAGAGTTTCATACAAAGAACTCCGAAGGGCGACATATGGATTTGCTGAAACTAACTTACTTGGCACTGGGGGTCTTGGCTCGGTTTATAGAGGGACACTGTCAGATGGGATTTTAGTGGCCATTAAGGTATTCAATGTACAGTTGGAAGAGGCTTTGACAAGTTTTGACATGGAATGTAAAATGCTAAGTACTATACGTCATCGAAATCTTATCAAAATCATCAGTTGTTGCATTGATATTGATTTTAGAGCTTTGGTACTTGGCTACATGCCTAATGGGAGCCTTGAGAAGTGGTTGTACTCTGATAACTTTTCGCTGAGTATCCTGCAGAGGTTGAATATTTTGATAGATGTTGCATTGGCATTGGAATACCTCCATCATGGTTACGAAACAACGATTGTCCATTGTGATTTGAAGCCCAGCAACATACTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGCATTGCGAAGCTCTTAGGTGGAGGAGGTTCTATGTCTCAAACCACGACCCTAGCTACAATTGGGTATATGGCTCCAGAGTATGGAATGGGAGGAATAGTTTCCAGAAGAGGGGATGTGTATAGTTTTGGTATTATACTGATGGAAACATTCACAAAAAGGAAGCCAACAGATGACATGTTTGTTGGACAAATGAATCTAAAGCAATGGGTTGCAAATTCATTGCTTTCGGATGCAATAGTTGAAGTTGTGGATTCCAATTTAATTGGGACTCAAGAAGAAGATGAAGATTTTGTGAGCAAGAGGTATTGTTTGTCATGCATTATGAATTTAGCCATGGCTTGTTGTGAAGAATCACCAGAAAATAGGATGAATATACAAGATGCTCTCTCCACGCTCAATAAAATCAAAAAAAAGTTTTTGAAAGACACTGCAGGAGGTATGCTACCAAACGTCCTCTTGTTCAGCAGACGTTCCATTGAGGAATTCTATAGCATATTTCATGGCTTTTAA

Protein Analysis

1011

Amino Acids

110.92

Weight (kDa)

6.1

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 81 3.8e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 166 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 144 - 194 3.2e-07 Leucine rich repeat
LRR_14 PF23598 326 - 439 5e-07 Leucine-rich repeat region
LRR_8 PF13855 457 - 516 5e-06 Leucine rich repeat
LRR_8 PF13855 530 - 588 1.2e-09 Leucine rich repeat
Pkinase PF00069 701 - 972 7.7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 703 - 972 3.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1860
Acc36I ACCTGC 2 cut(s) 1846, 1860
AccB1I GGYRCC 1 cut(s) 299
AccB7I CCANNNNNTGG 2 cut(s) 1784, 2606
AccBSI CCGCTC 1 cut(s) 919
AccI GTMKAC 2 cut(s) 69, 397
AciI CCGC 3 cut(s) 311, 525, 919
AclWI GGATC 3 cut(s) 1198, 1936, 1949
AcoI YGGCCR 1 cut(s) 2172
AcsI RAATTY 8 cut(s) 956, 1377, 1664, 1744, 1773, 2740, 2851, 3008
AfaI GTAC 8 cut(s) 111, 497, 589, 1888, 2193, 2245, 2313, 2354
AfiI CCNNNNNNNGG 9 cut(s) 856, 1431, 1453, 1549, 1784, 1855, 1974, 2606, 2634
AflIII ACRYGT 4 cut(s) 684, 1110, 2509, 2700
AjnI CCWGG 2 cut(s) 855, 1936
Alw26I GTCTC 4 cut(s) 130, 396, 1716, 2565
AlwI GGATC 3 cut(s) 1198, 1936, 1949
AoxI GGCC 5 cut(s) 658, 751, 847, 1965, 2172
ApeKI GCWGC 3 cut(s) 527, 1149, 2012
ApoI RAATTY 8 cut(s) 956, 1377, 1664, 1744, 1773, 2740, 2851, 3008
Asp700I GAANNNNTTC 2 cut(s) 2059, 2623
AspA2I CCTAGG 1 cut(s) 1297
AspS9I GGNCC 5 cut(s) 204, 274, 371, 465, 1392
AsuII TTCGAA 1 cut(s) 1794
AsuNHI GCTAGC 1 cut(s) 86
AvaII GGWCC 5 cut(s) 204, 274, 371, 465, 1392
AvrII CCTAGG 1 cut(s) 1297
BalI TGGCCA 1 cut(s) 2174
BamHI GGATCC 1 cut(s) 1941
BanI GGYRCC 1 cut(s) 299
BarI GAAGNNNNNNTAC 6 cut(s) 597, 629, 960, 992, 2336, 2368
BbsI GAAGAC 1 cut(s) 1219
BbvI GCAGC 3 cut(s) 514, 1161, 1999
BccI CCATC 9 cut(s) 460, 539, 676, 830, 1426, 1760, 2153, 2439, 2659
BciT130I CCWGG 2 cut(s) 857, 1938
BciVI GTATCC 1 cut(s) 2387
BcoDI GTCTC 4 cut(s) 130, 396, 1716, 2565
BfaI CTAG 9 cut(s) 87, 317, 851, 888, 1298, 1388, 1604, 2489, 2576
BfmI CTRYAG 6 cut(s) 96, 623, 1853, 2381, 2955, 3013
BfuAI ACCTGC 2 cut(s) 1846, 1860
BfuI GTATCC 1 cut(s) 2387
BglII AGATCT 2 cut(s) 562, 1585
BisI GCNGC 4 cut(s) 528, 920, 1150, 2013
BlnI CCTAGG 1 cut(s) 1297
BlpI GCTNAGC 1 cut(s) 988
BlsI GCNGC 4 cut(s) 529, 921, 1151, 2014
BmcAI AGTACT 2 cut(s) 1888, 2245
Bme1390I CCNGG 2 cut(s) 857, 1938
Bme18I GGWCC 5 cut(s) 204, 274, 371, 465, 1392
BmgT120I GGNCC 5 cut(s) 204, 274, 371, 465, 1392
BmiI GGNNCC 9 cut(s) 301, 373, 466, 517, 1393, 1860, 1943, 2336, 2598
BmrFI CCNGG 2 cut(s) 857, 1938
BmrI ACTGGG 2 cut(s) 235, 2130
BmsI GCATC 6 cut(s) 174, 794, 1376, 1898, 2746, 2896
BmtI GCTAGC 1 cut(s) 90
BmuI ACTGGG 2 cut(s) 235, 2130
BpiI GAAGAC 1 cut(s) 1219
BplI GAGNNNNNCTC 2 cut(s) 1873, 1905
BpmI CTGGAG 2 cut(s) 948, 2583
Bpu10I CCTNAGC 3 cut(s) 416, 1351, 1948
Bpu1102I GCTNAGC 1 cut(s) 988
Bpu14I TTCGAA 1 cut(s) 1794
BpuEI CTTGAG 5 cut(s) 935, 1165, 1261, 2360, 2781
BsaJI CCNNGG 8 cut(s) 367, 486, 754, 856, 1297, 1765, 1937, 2859
BsaXI ACNNNNNCTCC 2 cut(s) 2607, 2637
Bsc4I CCNNNNNNNGG 9 cut(s) 856, 1431, 1453, 1549, 1784, 1855, 1974, 2606, 2634
Bse1I ACTGG 4 cut(s) 206, 230, 850, 2125
Bse3DI GCAATG 3 cut(s) 2526, 2735, 2744
BseBI CCWGG 2 cut(s) 857, 1938
BseDI CCNNGG 8 cut(s) 367, 486, 754, 856, 1297, 1765, 1937, 2859
BseGI GGATG 6 cut(s) 668, 1463, 1655, 2644, 2761, 2898
BseLI CCNNNNNNNGG 9 cut(s) 856, 1431, 1453, 1549, 1784, 1855, 1974, 2606, 2634
BseMI GCAATG 3 cut(s) 2526, 2735, 2744
BseMII CTCAG 5 cut(s) 741, 814, 1503, 1747, 2363
BseNI ACTGG 4 cut(s) 206, 230, 850, 2125
BseRI GAGGAG 1 cut(s) 2562
BseXI GCAGC 3 cut(s) 514, 1161, 1999
BseYI CCCAGC 2 cut(s) 230, 2474
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 5 cut(s) 660, 753, 849, 1967, 2174
BshNI GGYRCC 1 cut(s) 299
BslFI GGGAC 4 cut(s) 357, 650, 2161, 2806
BslI CCNNNNNNNGG 9 cut(s) 856, 1431, 1453, 1549, 1784, 1855, 1974, 2606, 2634
BsmAI GTCTC 4 cut(s) 130, 396, 1716, 2565
BsmFI GGGAC 4 cut(s) 357, 650, 2161, 2806
BsmI GAATGC 2 cut(s) 835, 2008
BsnI GGCC 5 cut(s) 660, 753, 849, 1967, 2174
Bsp119I TTCGAA 1 cut(s) 1794
Bsp1407I TGTACA 1 cut(s) 2191
Bsp143I GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
Bsp1720I GCTNAGC 1 cut(s) 988
Bsp19I CCATGG 2 cut(s) 1765, 2859
BspACI CCGC 3 cut(s) 311, 525, 919
BspANI GGCC 5 cut(s) 660, 753, 849, 1967, 2174
BspCNI CTCAG 5 cut(s) 740, 813, 1502, 1746, 2364
BspLI GGNNCC 9 cut(s) 301, 373, 466, 517, 1393, 1860, 1943, 2336, 2598
BspMAI CTGCAG 3 cut(s) 1857, 2385, 2959
BspMI ACCTGC 2 cut(s) 1846, 1860
BspOI GCTAGC 1 cut(s) 90
BspPI GGATC 3 cut(s) 1198, 1936, 1949
BspQI GCTCTTC 2 cut(s) 150, 1488
BspT104I TTCGAA 1 cut(s) 1794
BspT107I GGYRCC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 919
BsrDI GCAATG 3 cut(s) 2526, 2735, 2744
BsrGI TGTACA 1 cut(s) 2191
BsrI ACTGG 4 cut(s) 206, 230, 850, 2125
BssECI CCNNGG 8 cut(s) 367, 486, 754, 856, 1297, 1765, 1937, 2859
BssMI GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
BssNAI GTATAC 1 cut(s) 70
BssT1I CCWWGG 6 cut(s) 367, 486, 754, 1297, 1765, 2859
Bst1107I GTATAC 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 857, 1938
Bst4CI ACNGT 7 cut(s) 77, 257, 500, 1866, 1896, 2154, 2196
Bst6I CTCTTC 5 cut(s) 150, 1488, 2050, 2196, 2626
BstAPI GCANNNNNTGC 2 cut(s) 2012, 2513
BstAUI TGTACA 1 cut(s) 2191
BstBI TTCGAA 1 cut(s) 1794
BstC8I GCNNGC 5 cut(s) 88, 163, 250, 525, 893
BstDSI CCRYGG 2 cut(s) 1765, 2859
BstEII GGTNACC 1 cut(s) 320
BstENI CCTNNNNNAGG 1 cut(s) 1429
BstF5I GGATG 6 cut(s) 668, 1463, 1655, 2644, 2761, 2898
BstKTI GATC 9 cut(s) 192, 343, 565, 613, 1158, 1193, 1360, 1588, 1944
BstMAI GTCTC 4 cut(s) 130, 396, 1716, 2565
BstMBI GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
BstMWI GCNNNNNNNGC 4 cut(s) 92, 1146, 2012, 2513
BstNI CCWGG 2 cut(s) 857, 1938
BstNSI RCATGY 5 cut(s) 688, 1114, 2326, 2513, 2704
BstPI GGTNACC 1 cut(s) 320
BstSCI CCNGG 2 cut(s) 855, 1936
BstSFI CTRYAG 6 cut(s) 96, 623, 1853, 2381, 2955, 3013
BstV1I GCAGC 3 cut(s) 514, 1161, 1999
BstV2I GAAGAC 1 cut(s) 1219
BstX2I RGATCY 3 cut(s) 562, 1585, 1941
BstYI RGATCY 3 cut(s) 562, 1585, 1941
BstZ17I GTATAC 1 cut(s) 70
BsuI GTATCC 1 cut(s) 2387
BsuRI GGCC 5 cut(s) 660, 753, 849, 1967, 2174
BtgI CCRYGG 2 cut(s) 1765, 2859
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 6 cut(s) 668, 1463, 1655, 2644, 2761, 2898
BtsI GCAGTG 1 cut(s) 2952
BtsIMutI CAGTG 7 cut(s) 808, 843, 1862, 1892, 2118, 2150, 2952
BveI ACCTGC 2 cut(s) 1846, 1860
Cac8I GCNNGC 5 cut(s) 88, 163, 250, 525, 893
Cfr13I GGNCC 5 cut(s) 204, 274, 371, 465, 1392
Csp6I GTAC 8 cut(s) 110, 496, 588, 1887, 2192, 2244, 2312, 2353
CviQI GTAC 8 cut(s) 110, 496, 588, 1887, 2192, 2244, 2312, 2353
DpnI GATC 9 cut(s) 191, 342, 564, 612, 1157, 1192, 1359, 1587, 1943
DpnII GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
EaeI YGGCCR 1 cut(s) 2172
Eam1104I CTCTTC 5 cut(s) 150, 1488, 2050, 2196, 2626
EarI CTCTTC 5 cut(s) 150, 1488, 2050, 2196, 2626
Eco130I CCWWGG 6 cut(s) 367, 486, 754, 1297, 1765, 2859
Eco147I AGGCCT 1 cut(s) 753
Eco32I GATATC 1 cut(s) 705
Eco47I GGWCC 5 cut(s) 204, 274, 371, 465, 1392
Eco91I GGTNACC 1 cut(s) 320
EcoNI CCTNNNNNAGG 1 cut(s) 1429
EcoO109I RGGNCCY 2 cut(s) 371, 465
EcoO65I GGTNACC 1 cut(s) 320
EcoRI GAATTC 3 cut(s) 1377, 1664, 3008
EcoRII CCWGG 2 cut(s) 855, 1936
EcoRV GATATC 1 cut(s) 705
EcoT14I CCWWGG 6 cut(s) 367, 486, 754, 1297, 1765, 2859
EcoT22I ATGCAT 4 cut(s) 787, 1369, 1913, 2846
ErhI CCWWGG 6 cut(s) 367, 486, 754, 1297, 1765, 2859
FaqI GGGAC 4 cut(s) 357, 650, 2161, 2806
FauI CCCGC 1 cut(s) 532
FauNDI CATATG 2 cut(s) 1973, 2089
FblI GTMKAC 2 cut(s) 69, 397
Fnu4HI GCNGC 4 cut(s) 528, 920, 1150, 2013
FokI GGATG 6 cut(s) 655, 1450, 1662, 2651, 2768, 2905
Fsp4HI GCNGC 4 cut(s) 528, 920, 1150, 2013
FspBI CTAG 9 cut(s) 87, 317, 851, 888, 1298, 1388, 1604, 2489, 2576
GluI GCNGC 4 cut(s) 528, 920, 1150, 2013
GsaI CCCAGC 2 cut(s) 234, 2478
GsuI CTGGAG 2 cut(s) 948, 2583
HaeIII GGCC 5 cut(s) 660, 753, 849, 1967, 2174
HincII GTYRAC 1 cut(s) 1404
HindII GTYRAC 1 cut(s) 1404
HindIII AAGCTT 1 cut(s) 1657
HpaI GTTAAC 1 cut(s) 1404
Hpy166II GTNNAC 8 cut(s) 70, 207, 398, 690, 1404, 1481, 1832, 1844
Hpy188I TCNGA 9 cut(s) 1027, 1128, 1172, 1492, 1736, 2078, 2158, 2359, 2755
Hpy8I GTNNAC 8 cut(s) 70, 207, 398, 690, 1404, 1481, 1832, 1844
HpyAV CCTTC 6 cut(s) 170, 1353, 1616, 1775, 1978, 2073
HpyCH4III ACNGT 7 cut(s) 77, 257, 500, 1866, 1896, 2154, 2196
HpyCH4IV ACGT 3 cut(s) 2251, 2976, 2995
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 1146, 2012, 2513
HpySE526I ACGT 3 cut(s) 2251, 2976, 2995
KspAI GTTAAC 1 cut(s) 1404
Kzo9I GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
LguI GCTCTTC 2 cut(s) 150, 1488
LmnI GCTCC 4 cut(s) 313, 1787, 2334, 2602
Lsp1109I GCAGC 3 cut(s) 514, 1161, 1999
LweI GCATC 6 cut(s) 174, 794, 1376, 1898, 2746, 2896
MaeI CTAG 9 cut(s) 87, 317, 851, 888, 1298, 1388, 1604, 2489, 2576
MaeII ACGT 3 cut(s) 2251, 2976, 2995
MalI GATC 9 cut(s) 191, 342, 564, 612, 1157, 1192, 1359, 1587, 1943
MbiI CCGCTC 1 cut(s) 919
MboI GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
MfeI CAATTG 1 cut(s) 2583
MflI RGATCY 3 cut(s) 562, 1585, 1941
MlsI TGGCCA 1 cut(s) 2174
MluNI TGGCCA 1 cut(s) 2174
MlyI GAGTC 5 cut(s) 146, 270, 420, 1755, 2788
MmeI TCCRAC 3 cut(s) 1223, 2178, 2689
Mox20I TGGCCA 1 cut(s) 2174
Mph1103I ATGCAT 4 cut(s) 787, 1369, 1913, 2846
MroXI GAANNNNTTC 2 cut(s) 2059, 2623
MscI TGGCCA 1 cut(s) 2174
MseI TTAA 9 cut(s) 5, 17, 654, 1097, 1236, 1403, 2178, 2786, 3034
Msp20I TGGCCA 1 cut(s) 2174
MspA1I CMGCKG 2 cut(s) 527, 1288
MspR9I CCNGG 2 cut(s) 857, 1938
MunI CAATTG 1 cut(s) 2583
Mva1269I GAATGC 2 cut(s) 835, 2008
MvaI CCWGG 2 cut(s) 857, 1938
MwoI GCNNNNNNNGC 4 cut(s) 92, 1146, 2012, 2513
NcoI CCATGG 2 cut(s) 1765, 2859
NdeI CATATG 2 cut(s) 1973, 2089
NdeII GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
NheI GCTAGC 1 cut(s) 86
NlaIV GGNNCC 9 cut(s) 301, 373, 466, 517, 1393, 1860, 1943, 2336, 2598
NmuCI GTSAC 4 cut(s) 633, 1301, 1516, 2020
NsiI ATGCAT 4 cut(s) 787, 1369, 1913, 2846
NspI RCATGY 5 cut(s) 688, 1114, 2326, 2513, 2704
NspV TTCGAA 1 cut(s) 1794
PaqCI CACCTGC 1 cut(s) 1860
PceI AGGCCT 1 cut(s) 753
PciI ACATGT 4 cut(s) 684, 1110, 2509, 2700
PciSI GCTCTTC 2 cut(s) 150, 1488
PctI GAATGC 2 cut(s) 835, 2008
PdmI GAANNNNTTC 2 cut(s) 2059, 2623
PfeI GAWTC 7 cut(s) 932, 1123, 1702, 1811, 2719, 2777, 2876
PflFI GACNNNGTC 1 cut(s) 2152
PflMI CCANNNNNTGG 2 cut(s) 1784, 2606
PkrI GCNGC 4 cut(s) 529, 921, 1151, 2014
PleI GAGTC 5 cut(s) 145, 269, 420, 1755, 2788
PpsI GAGTC 5 cut(s) 145, 269, 420, 1755, 2788
PpuMI RGGWCCY 2 cut(s) 371, 465
PscI ACATGT 4 cut(s) 684, 1110, 2509, 2700
Psp5II RGGWCCY 2 cut(s) 371, 465
Psp6I CCWGG 2 cut(s) 855, 1936
PspEI GGTNACC 1 cut(s) 320
PspFI CCCAGC 2 cut(s) 230, 2474
PspGI CCWGG 2 cut(s) 855, 1936
PspN4I GGNNCC 9 cut(s) 301, 373, 466, 517, 1393, 1860, 1943, 2336, 2598
PspPI GGNCC 5 cut(s) 204, 274, 371, 465, 1392
PspPPI RGGWCCY 2 cut(s) 371, 465
PstI CTGCAG 3 cut(s) 1857, 2385, 2959
PsuI RGATCY 3 cut(s) 562, 1585, 1941
PsyI GACNNNGTC 1 cut(s) 2152
PvuII CAGCTG 1 cut(s) 1288
RsaI GTAC 8 cut(s) 111, 497, 589, 1888, 2193, 2245, 2313, 2354
RsaNI GTAC 8 cut(s) 110, 496, 588, 1887, 2192, 2244, 2312, 2353
SapI GCTCTTC 2 cut(s) 150, 1488
SaqAI TTAA 9 cut(s) 5, 17, 654, 1097, 1236, 1403, 2178, 2786, 3034
SatI GCNGC 4 cut(s) 528, 920, 1150, 2013
Sau3AI GATC 9 cut(s) 189, 340, 562, 610, 1155, 1190, 1357, 1585, 1941
Sau96I GGNCC 5 cut(s) 204, 274, 371, 465, 1392
SbfI CCTGCAGG 1 cut(s) 1857
ScaI AGTACT 2 cut(s) 1888, 2245
SchI GAGTC 5 cut(s) 146, 270, 420, 1755, 2788
ScrFI CCNGG 2 cut(s) 857, 1938
SdaI CCTGCAGG 1 cut(s) 1857
SfaNI GCATC 6 cut(s) 174, 794, 1376, 1898, 2746, 2896
SfcI CTRYAG 6 cut(s) 96, 623, 1853, 2381, 2955, 3013
SfuI TTCGAA 1 cut(s) 1794
SinI GGWCC 5 cut(s) 204, 274, 371, 465, 1392
SmlI CTYRAG 5 cut(s) 914, 1144, 1240, 2339, 2796
SmoI CTYRAG 5 cut(s) 914, 1144, 1240, 2339, 2796
Sse8387I CCTGCAGG 1 cut(s) 1857
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 3 cut(s) 311, 525, 919
SspI AATATT 1 cut(s) 2395
SspMI CTAG 9 cut(s) 87, 317, 851, 888, 1298, 1388, 1604, 2489, 2576
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 2 cut(s) 855, 1936
StyI CCWWGG 6 cut(s) 367, 486, 754, 1297, 1765, 2859
TaaI ACNGT 7 cut(s) 77, 257, 500, 1866, 1896, 2154, 2196
TaiI ACGT 3 cut(s) 2254, 2979, 2998
TaqI TCGA 9 cut(s) 34, 264, 788, 827, 935, 1556, 1794, 1823, 2257
TatI WGTACW 5 cut(s) 109, 1886, 2191, 2243, 2352
TauI GCSGC 1 cut(s) 922
TfiI GAWTC 7 cut(s) 932, 1123, 1702, 1811, 2719, 2777, 2876
Tru1I TTAA 9 cut(s) 5, 17, 654, 1097, 1236, 1403, 2178, 2786, 3034
Tru9I TTAA 9 cut(s) 5, 17, 654, 1097, 1236, 1403, 2178, 2786, 3034
TscAI CASTG 7 cut(s) 808, 850, 1869, 1899, 2125, 2157, 2959
TseFI GTSAC 4 cut(s) 633, 1301, 1516, 2020
TseI GCWGC 3 cut(s) 527, 1149, 2012
Tsp45I GTSAC 4 cut(s) 633, 1301, 1516, 2020
TspRI CASTG 7 cut(s) 808, 850, 1869, 1899, 2125, 2157, 2959
Tth111I GACNNNGTC 1 cut(s) 2152
Van91I CCANNNNNTGG 2 cut(s) 1784, 2606
VpaK11BI GGWCC 5 cut(s) 204, 274, 371, 465, 1392
XagI CCTNNNNNAGG 1 cut(s) 1429
XapI RAATTY 8 cut(s) 956, 1377, 1664, 1744, 1773, 2740, 2851, 3008
XceI RCATGY 5 cut(s) 688, 1114, 2326, 2513, 2704
XcmI CCANNNNNNNNNTGG 2 cut(s) 1425, 2046
XmaJI CCTAGG 1 cut(s) 1297
XmiI GTMKAC 2 cut(s) 69, 397
XmnI GAANNNNTTC 2 cut(s) 2059, 2623
XspI CTAG 9 cut(s) 87, 317, 851, 888, 1298, 1388, 1604, 2489, 2576
ZrmI AGTACT 2 cut(s) 1888, 2245
Zsp2I ATGCAT 4 cut(s) 787, 1369, 1913, 2846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.