Rw4G009490

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000058
Physical Location & Seq
Forward (+)
21433460 .. 21437523
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009490.1

Sequence Viewer

Length: 3357 bp
ATGGAAAAGCTACAAACTTTCCTTTTTGGAGTAGCGCTACTACACTACTCATGCTTCCTTAAGTTGGCCATATCTTCACCTTCCAACTTCACTGATCAGTCTGCTTTGCTTGCCATTCAATCTCAAATCACTTTTGACCCCACCAATACTGTCTTGGGTGGTGGTAGCTGGATCACCAAAACAAGCTTCTGTAATTGGTTTGGGGTCTCTTGCAGTAAACGCAGGCAAAGAGTCACAGCCTTGGACCTTTCCTATATGAATCTCCAAGGCACCATTTCCCCTCATGTTGGCAATCTCTCCTTCCTAATCTCACTTGATCTCCGCAATAACAGCTTCCTGGGGTTCATACCACATGAAATCAGCCGTTTGCATCGCTTGAGGATACTTGTACTTCAAGCTAACAAGTTGGAAGGAAACATCCCTCCAACTTTACAGCTTTGCCACAAGCTCGAGTCCATATCCCTTTCAATAAACAGATTAAGTGGTCTCATACCTAAAGAGTTGGGTTTCCTTCCCAGGGTTCGTAAATTATATCTCGGTGCAAACAATCTCACAGGTGAAATTCCTTCGTCTCTGGGTAACATTTCAACCTTACAAGAATTAAGTCTGGCCAAAAATGGTCTCACAGGTTCATTCCCTTCTGCTCTACTCAACGTATCTAATCTAGCTCTTATTTCCCTATCTGGCAATTACATGTCAGGTAGTCTTCCTGTAGATCTTTGCCGTAATTGGCCTAATACTTATTTCATTTCTCTATCTGACAACAAATTCGGTGGCCAACTGACTTTCGGAATAAGCCAATGTAGAGAGCTTGAGATCTTATCTCTGTCATACAATAATTTTGTCGGAAGTATTCCGGGAGAAATTGGGAGTTTACAACATCTTAAAACTCTATTTCTTGGTGTCAATAACCTAACTGGTGCTATACCTCCAACAATAGGTAACCTGTCAAAGTTGCAGTTCTTTACTGTGCCAGATAATAATTTAGAGGGAAGTATTCCAAGTGATTTGGGGCATCTTTCTAAACTAAATTATCTCTTTTTTGCTCGGAATAGCCTAACAGGTGAAATACCTGAGTCCATTTTCAATATCTCCTCCTTAAGTACTTTACAAGTACAAACTAATGCTCTTTCTGGAGAATTTCCTTCTCGAGCTATGATTTCTAACCTCAATACCCTATACTGTTATACCAATCACATTACAGGAAATCTACCATCCTATTTTTCAAATTTAAGCCAGCTTGTTGAGCTTGATGTTTCCAACAACTCACTTTATGGACCAATACCCATGAACCTTGGTAGCTCAAAATACCTCGAATTCCTTAACTTGGGAGGAAATCAGTTGTCAGGGGAACCTGGAGTTACTGTACTTAGGTTCCTTTCTTCTATATTTAATAGCAGTTCACTGAAACATTTATTTTTGAGTAGAAATCCTCTAGACGGGATCATGCCCGATTCATTTGGAAATTTCTCATATTCCCTCGAGGTATTTTTTGCAACTGCCTGCCAGATTAAGGGCCCTATACCCAAGACGATTCAGTTGTTGAAAAATCTGACCTTCCTTGCCTTAAGTGACAACAATATTAGTGGGAAAATACCATCATCAATTGGAGCATTGGAAATGTTGCAGAGATTGTATCTTGAAAACAACAAGATTGAAGGGCTCATTCCTAATGAGCTTTGTCTTTTGCGGAACCTGGGAGATCTATCACTAGGAAATAATCAAATCTCTGGATCAATCCCAAATTGCATTGCAAATCTCAGTCGCATTCAAAGACTTCTTCTAAGTTCCAATAGATTGACATCTGCAGTACCAGTGAATTTATGGAATCTTGAAAATCTTCTGTTCTTAAATTTGTCATCCAATATTTTCAGTGGATACTTACCTCCAAGCATGAAAAGGTCGGTTGCTGTTCAGATGGTCGACTTATCTGACAATCAAGTTACTGGGAACATTTCAAGCATTGTCGGAGCCTTCCCAAGCATAAACTCTCTAAGCTTAGCAAACAACTTGTTTATAGGATCTCTTCCGCAAACTTTGGGTGATCTGAAAGGATTGGAGTTTTTGGACCTATCAAACAATAATCTGTCTGGGCCAATTCCAAAGTCTCTTGAAACACTTAAATATCTGAACTACTTAAATTTATCTTTTAATCAACTCTCTGGAGAGATTCCTTCTAGTGGAGCTTTTATCAACTTCACTGCCCTATCATTTTTGGAGAATAGAGGCCTTTGTGGGAGACCGGAGTTTGGAGTCCCACCTTGTATGGCTCTTGGAACTCAGAAGTCAAAGATGGCAAATCATTTACTCAAGTATGTTCTTCCAGGCATTGCATCAACAATGATCTTGATAGCTCTCATATACATGGTGACAAAGTGTCGAAAAAGAGATGAAGGCATTCCAAGTTCAGTACAACGGTTAACAACAACAGAGCATAGAATGATATCATATCATCAACTCCGTCGTGCTACAAATGGCTTCTGTGAAAGCAACTTGCTTGGAGTTGGAAGTTTTGGCTCTGTATACAAGGGGATTTTAACAGATGGGACAATTGTTGCTGTTAAAGTTCTAAATTTGCAAATGGAGGGAGCCTTTAAAAGTTTTGATGCAGAATGTAAAGTTTGGCGGAGAATTCGGCACAGAAATCTTGTCAAGATCATGACTACATGCTCTAACCATGAGGTAAGAGCTTTGGTGCTGCAATACATGTCTAATGGCAGCCTTGAGAAGTGGCTATACTCTCATAACTATTGTTTGACTCTTCTGCAAAGAGTGAGCATGTTGATCGACATTGCACTGGCTTTGGACTATCTCCATCATGGTCAATCAGAAGGTGTGGTGCACTGTGATTTGAAGCCTAGCAATATCCTTCTCGATGAAGACATGGTTGCACACGTTGGAGATTTCGGGCTTGCAAAAATTATAGCAATTAACAAAGATGCAACACAGACTAGAACTCTCGGCACACTCGGCTACGTTGCACCAGAGTACGGTTCTGCAGGAGTGGTGTCCGCAAGAGGTGATATTTATAGTTTTGGGATAATGTTGATGGAAATAATTGTGAGAAAGAAACCCACGGATGAAATGTTTGCTGGAGAACTGACTTTGAGACAATGGATAAATGCTTCGCTTCCTGACAGTGTTCTCGAAGTAGTGGATGCTGGCCTATTGAGCATAGAAGATGAAACTAGAGACATGAATGCCACAGTACAAACCATTTTGTCAATACTGGAAATAGGTTTGGGATGTTCTGAAGAAGTGGCTCAGGAAAGAATCAACATTAAGGATGTAGTGCCCAAGCTCACGAAAATAAAATGTGCACTAGAAGATAGAAATGGCGGTGTTTATTATCCATGA

Protein Analysis

1118

Amino Acids

122.61

Weight (kDa)

7.95

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 81 - 227 1e-07 Leucine-rich repeat region
LRR_8 PF13855 269 - 329 7.1e-06 Leucine rich repeat
LRR_14 PF23598 269 - 346 2.8e-06 Leucine-rich repeat region
LRR_14 PF23598 335 - 443 2.1e-06 Leucine-rich repeat region
LRR_14 PF23598 508 - 620 3.8e-09 Leucine-rich repeat region
LRR_8 PF13855 660 - 720 1.1e-06 Leucine rich repeat
Pkinase PF00069 830 - 1027 1.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 831 - 1029 4.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1481
AccB1I GGYRCC 1 cut(s) 269
AccI GTMKAC 2 cut(s) 1923, 2523
AciI CCGC 6 cut(s) 322, 1690, 2030, 2626, 3012, 3339
AclWI GGATC 4 cut(s) 179, 1451, 1741, 2029
AcoI YGGCCR 3 cut(s) 66, 609, 775
AcuI CTGAAG 1 cut(s) 3273
AfaI GTAC 8 cut(s) 390, 1105, 1116, 1368, 1812, 2412, 2990, 3210
AfeI AGCGCT 1 cut(s) 36
AflII CTTAAG 3 cut(s) 59, 1099, 1567
AflIII ACRYGT 3 cut(s) 693, 2706, 2893
AhdI GACNNNNNGTC 1 cut(s) 2376
AjnI CCWGG 5 cut(s) 336, 515, 1354, 1695, 2323
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
Alw21I GWGCWC 2 cut(s) 2844, 3322
Alw26I GTCTC 8 cut(s) 211, 491, 576, 626, 2112, 2233, 3103, 3186
Alw44I GTGCAC 2 cut(s) 2840, 3318
AlwI GGATC 4 cut(s) 179, 1451, 1741, 2029
Ama87I CYCGRG 3 cut(s) 449, 1149, 1481
Aor51HI AGCGCT 1 cut(s) 36
AoxI GGCC 8 cut(s) 66, 609, 731, 775, 1516, 2093, 2227, 3163
ApaI GGGCCC 1 cut(s) 1520
ApaLI GTGCAC 2 cut(s) 2840, 3318
ApeKI GCWGC 2 cut(s) 2698, 2718
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
Asp700I GAANNNNTTC 2 cut(s) 1839, 2397
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 6 cut(s) 244, 1277, 1516, 1517, 2068, 2093
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 7 cut(s) 69, 166, 569, 1076, 2054, 2380, 3032
AvaI CYCGRG 3 cut(s) 449, 1149, 1481
AvaII GGWCC 3 cut(s) 244, 1277, 2068
BaeGI GKGCMC 4 cut(s) 1520, 2844, 3297, 3322
BalI TGGCCA 3 cut(s) 68, 611, 777
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 2 cut(s) 1520, 1665
BarI GAAGNNNNNNTAC 2 cut(s) 2720, 2752
BbsI GAAGAC 2 cut(s) 698, 2886
Bbv12I GWGCWC 2 cut(s) 2844, 3322
BbvI GCAGC 2 cut(s) 2685, 2730
BccI CCATC 7 cut(s) 1222, 1606, 1912, 2287, 2537, 2823, 3043
BceAI ACGGC 2 cut(s) 348, 708
BcgI CGANNNNNNTGC 2 cut(s) 2767, 2801
BciT130I CCWGG 5 cut(s) 338, 517, 1356, 1697, 2325
BciVI GTATCC 2 cut(s) 375, 1871
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 858
BcoDI GTCTC 8 cut(s) 211, 491, 576, 626, 2112, 2233, 3103, 3186
BfaI CTAG 8 cut(s) 665, 1436, 1712, 2178, 2859, 2952, 3189, 3323
BfmI CTRYAG 3 cut(s) 711, 1806, 2997
BfoI RGCGCY 1 cut(s) 38
BfrI CTTAAG 3 cut(s) 59, 1099, 1567
BfuI GTATCC 2 cut(s) 375, 1871
BglII AGATCT 3 cut(s) 715, 816, 1702
BisI GCNGC 2 cut(s) 2699, 2719
BlpI GCTNAGC 1 cut(s) 1999
BlsI GCNGC 2 cut(s) 2700, 2720
BmcAI AGTACT 1 cut(s) 1105
Bme1390I CCNGG 6 cut(s) 338, 517, 858, 1356, 1697, 2325
Bme18I GGWCC 3 cut(s) 244, 1277, 2068
BmeRI GACNNNNNGTC 1 cut(s) 2376
BmeT110I CYCGRG 3 cut(s) 449, 1149, 1481
BmgT120I GGNCC 6 cut(s) 244, 1277, 1516, 1517, 2068, 2093
BmiI GGNNCC 7 cut(s) 271, 1353, 1376, 1518, 1694, 1972, 2590
BmrFI CCNGG 6 cut(s) 338, 517, 858, 1356, 1697, 2325
BmrI ACTGGG 1 cut(s) 1956
BmsI GCATC 6 cut(s) 379, 1024, 2342, 2596, 2929, 3148
BmuI ACTGGG 1 cut(s) 1956
BpiI GAAGAC 2 cut(s) 698, 2886
BpmI CTGGAG 4 cut(s) 1155, 1377, 2184, 3114
Bpu10I CCTNAGC 1 cut(s) 3264
Bpu1102I GCTNAGC 1 cut(s) 1999
BpuEI CTTGAG 4 cut(s) 397, 833, 2294, 2744
BpuMI CCSGG 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 1801
BsaI GGTCTC 4 cut(s) 211, 491, 626, 2233
BsaJI CCNNGG 8 cut(s) 240, 265, 337, 515, 516, 1294, 1696, 3075
BsaWI WCCGGW 1 cut(s) 2242
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 1350, 1380
Bse1I ACTGG 5 cut(s) 922, 1814, 1951, 2802, 3234
Bse3DI GCAATG 3 cut(s) 1749, 2328, 2790
Bse8I GATNNNNATC 1 cut(s) 1801
BseBI CCWGG 5 cut(s) 338, 517, 1356, 1697, 2325
BseDI CCNNGG 8 cut(s) 240, 265, 337, 515, 516, 1294, 1696, 3075
BseGI GGATG 7 cut(s) 417, 1214, 1859, 3085, 3163, 3251, 3292
BseJI GATNNNNATC 1 cut(s) 1801
BseMI GCAATG 3 cut(s) 1749, 2328, 2790
BseMII CTCAG 4 cut(s) 1065, 1774, 2294, 3278
BseNI ACTGG 5 cut(s) 922, 1814, 1951, 2802, 3234
BseRI GAGGAG 1 cut(s) 1084
BseSI GKGCMC 4 cut(s) 1520, 2844, 3297, 3322
BseXI GCAGC 2 cut(s) 2685, 2730
BshFI GGCC 8 cut(s) 68, 611, 733, 777, 1518, 2095, 2229, 3165
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 2 cut(s) 2844, 3322
BsiHKCI CYCGRG 3 cut(s) 449, 1149, 1481
BsiSI CCGG 2 cut(s) 857, 2243
BslFI GGGAC 2 cut(s) 2240, 2560
BsmAI GTCTC 8 cut(s) 211, 491, 576, 626, 2112, 2233, 3103, 3186
BsmBI CGTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 2240, 2560
BsmI GAATGC 3 cut(s) 1767, 2397, 3205
BsnI GGCC 8 cut(s) 68, 611, 733, 777, 1518, 2095, 2229, 3165
Bso31I GGTCTC 4 cut(s) 211, 491, 626, 2233
BsoBI CYCGRG 3 cut(s) 449, 1149, 1481
Bsp120I GGGCCC 1 cut(s) 1516
Bsp1286I GDGCHC 5 cut(s) 1520, 1665, 2844, 3297, 3322
Bsp1720I GCTNAGC 1 cut(s) 1999
BspACI CCGC 6 cut(s) 322, 1690, 2030, 2626, 3012, 3339
BspANI GGCC 8 cut(s) 68, 611, 733, 777, 1518, 2095, 2229, 3165
BspCNI CTCAG 4 cut(s) 1066, 1773, 2293, 3277
BspHI TCATGA 1 cut(s) 2658
BspLI GGNNCC 7 cut(s) 271, 1353, 1376, 1518, 1694, 1972, 2590
BspMAI CTGCAG 2 cut(s) 1810, 3001
BspPI GGATC 4 cut(s) 179, 1451, 1741, 2029
BspT107I GGYRCC 1 cut(s) 269
BspTI CTTAAG 3 cut(s) 59, 1099, 1567
BspTNI GGTCTC 4 cut(s) 211, 491, 626, 2233
BsrDI GCAATG 3 cut(s) 1749, 2328, 2790
BsrI ACTGG 5 cut(s) 922, 1814, 1951, 2802, 3234
BssECI CCNNGG 8 cut(s) 240, 265, 337, 515, 516, 1294, 1696, 3075
BssNAI GTATAC 1 cut(s) 2524
BssT1I CCWWGG 3 cut(s) 240, 265, 1294
Bst1107I GTATAC 1 cut(s) 2524
Bst2UI CCWGG 5 cut(s) 338, 517, 1356, 1697, 2325
Bst4CI ACNGT 9 cut(s) 151, 970, 1184, 1366, 2418, 2846, 2993, 3140, 3208
Bst6I CTCTTC 2 cut(s) 2031, 2766
BstAFI CTTAAG 3 cut(s) 59, 1099, 1567
BstC8I GCNNGC 6 cut(s) 111, 224, 1238, 1504, 2913, 3163
BstDEI CTNAG 8 cut(s) 1074, 1370, 1760, 1784, 1994, 1999, 2280, 3264
BstDSI CCRYGG 1 cut(s) 3075
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 7 cut(s) 417, 1214, 1859, 3085, 3163, 3251, 3292
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 1 cut(s) 37
BstMAI GTCTC 8 cut(s) 211, 491, 576, 626, 2112, 2233, 3103, 3186
BstMWI GCNNNNNNNGC 6 cut(s) 110, 219, 330, 1246, 2970, 3171
BstNI CCWGG 5 cut(s) 338, 517, 1356, 1697, 2325
BstNSI RCATGY 4 cut(s) 697, 2670, 2710, 2782
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 6 cut(s) 336, 515, 856, 1354, 1695, 2323
BstSFI CTRYAG 3 cut(s) 711, 1806, 2997
BstSLI GKGCMC 4 cut(s) 1520, 2844, 3297, 3322
BstV1I GCAGC 2 cut(s) 2685, 2730
BstV2I GAAGAC 2 cut(s) 698, 2886
BstX2I RGATCY 4 cut(s) 715, 816, 1702, 2021
BstYI RGATCY 4 cut(s) 715, 816, 1702, 2021
BstZ17I GTATAC 1 cut(s) 2524
BsuI GTATCC 2 cut(s) 375, 1871
BsuRI GGCC 8 cut(s) 68, 611, 733, 777, 1518, 2095, 2229, 3165
BtgI CCRYGG 1 cut(s) 3075
BtgZI GCGATG 1 cut(s) 356
BtsCI GGATG 7 cut(s) 417, 1214, 1859, 3085, 3163, 3251, 3292
BtsI GCAGTG 1 cut(s) 2199
BtsIMutI CAGTG 8 cut(s) 90, 1403, 1821, 1879, 2199, 2795, 2842, 3145
Cac8I GCNNGC 6 cut(s) 111, 224, 1238, 1504, 2913, 3163
CciI TCATGA 1 cut(s) 2658
CfoI GCGC 1 cut(s) 37
Cfr13I GGNCC 6 cut(s) 244, 1277, 1516, 1517, 2068, 2093
Csp6I GTAC 8 cut(s) 389, 1104, 1115, 1367, 1811, 2411, 2989, 3209
CspCI CAANNNNNGTGG 4 cut(s) 754, 789, 1567, 1602
CviQI GTAC 8 cut(s) 389, 1104, 1115, 1367, 1811, 2411, 2989, 3209
DdeI CTNAG 8 cut(s) 1074, 1370, 1760, 1784, 1994, 1999, 2280, 3264
DraI TTTAAA 1 cut(s) 2596
DriI GACNNNNNGTC 1 cut(s) 2376
EaeI YGGCCR 3 cut(s) 66, 609, 775
Eam1104I CTCTTC 2 cut(s) 2031, 2766
Eam1105I GACNNNNNGTC 1 cut(s) 2376
EarI CTCTTC 2 cut(s) 2031, 2766
EciI GGCGGA 1 cut(s) 2641
Eco130I CCWWGG 3 cut(s) 240, 265, 1294
Eco147I AGGCCT 1 cut(s) 2229
Eco24I GRGCYC 2 cut(s) 1520, 1665
Eco31I GGTCTC 4 cut(s) 211, 491, 626, 2233
Eco32I GATATC 1 cut(s) 2445
Eco47I GGWCC 3 cut(s) 244, 1277, 2068
Eco47III AGCGCT 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 3273
Eco88I CYCGRG 3 cut(s) 449, 1149, 1481
Eco91I GGTNACC 1 cut(s) 941
EcoO109I RGGNCCY 2 cut(s) 1516, 1517
EcoO65I GGTNACC 1 cut(s) 941
EcoRI GAATTC 2 cut(s) 1316, 2631
EcoRII CCWGG 5 cut(s) 336, 515, 1354, 1695, 2323
EcoRV GATATC 1 cut(s) 2445
EcoT14I CCWWGG 3 cut(s) 240, 265, 1294
EcoT38I GRGCYC 2 cut(s) 1520, 1665
ErhI CCWWGG 3 cut(s) 240, 265, 1294
Esp3I CGTCTC 1 cut(s) 576
FaqI GGGAC 2 cut(s) 2240, 2560
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 1923, 2523
Fnu4HI GCNGC 2 cut(s) 2699, 2719
FokI GGATG 7 cut(s) 404, 1201, 1846, 3092, 3170, 3258, 3299
FriOI GRGCYC 2 cut(s) 1520, 1665
Fsp4HI GCNGC 2 cut(s) 2699, 2719
FspBI CTAG 8 cut(s) 665, 1436, 1712, 2178, 2859, 2952, 3189, 3323
GlaI GCGC 1 cut(s) 36
GluI GCNGC 2 cut(s) 2699, 2719
GsuI CTGGAG 4 cut(s) 1155, 1377, 2184, 3114
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 8 cut(s) 68, 611, 733, 777, 1518, 2095, 2229, 3165
HapII CCGG 2 cut(s) 857, 2243
HhaI GCGC 1 cut(s) 37
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 2 cut(s) 1924, 2421
HindII GTYRAC 2 cut(s) 1924, 2421
HindIII AAGCTT 2 cut(s) 184, 1996
HpaI GTTAAC 1 cut(s) 2421
HpaII CCGG 2 cut(s) 857, 2243
HphI GGTGA 7 cut(s) 69, 166, 569, 1076, 2054, 2380, 3032
Hpy166II GTNNAC 8 cut(s) 218, 875, 1403, 1924, 2421, 2524, 2842, 3320
Hpy8I GTNNAC 8 cut(s) 218, 875, 1403, 1924, 2421, 2524, 2842, 3320
Hpy99I CGWCG 1 cut(s) 2466
HpyCH4III ACNGT 9 cut(s) 151, 970, 1184, 1366, 2418, 2846, 2993, 3140, 3208
HpyCH4IV ACGT 3 cut(s) 654, 2895, 2976
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 219, 330, 1246, 2970, 3171
HpyF3I CTNAG 8 cut(s) 1074, 1370, 1760, 1784, 1994, 1999, 2280, 3264
HpySE526I ACGT 3 cut(s) 654, 2895, 2976
HspAI GCGC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 94
KspAI GTTAAC 1 cut(s) 2421
LmnI GCTCC 4 cut(s) 1610, 1970, 2183, 2588
Lsp1109I GCAGC 2 cut(s) 2685, 2730
LweI GCATC 6 cut(s) 379, 1024, 2342, 2596, 2929, 3148
MaeI CTAG 8 cut(s) 665, 1436, 1712, 2178, 2859, 2952, 3189, 3323
MaeII ACGT 3 cut(s) 654, 2895, 2976
MaeIII GTNAC 7 cut(s) 232, 578, 941, 1360, 1571, 1942, 2368
MfeI CAATTG 2 cut(s) 1605, 2550
MflI RGATCY 4 cut(s) 715, 816, 1702, 2021
MhlI GDGCHC 5 cut(s) 1520, 1665, 2844, 3297, 3322
MlsI TGGCCA 3 cut(s) 68, 611, 777
MluNI TGGCCA 3 cut(s) 68, 611, 777
MlyI GAGTC 5 cut(s) 240, 461, 1085, 2262, 2752
MmeI TCCRAC 9 cut(s) 108, 387, 449, 826, 956, 1284, 1948, 2485, 2878
Mox20I TGGCCA 3 cut(s) 68, 611, 777
MroXI GAANNNNTTC 2 cut(s) 1839, 2397
MscI TGGCCA 3 cut(s) 68, 611, 777
MslI CAYNNNNRTG 1 cut(s) 2363
Msp20I TGGCCA 3 cut(s) 68, 611, 777
MspCI CTTAAG 3 cut(s) 59, 1099, 1567
MspI CCGG 2 cut(s) 857, 2243
MspR9I CCNGG 6 cut(s) 338, 517, 858, 1356, 1697, 2325
MunI CAATTG 2 cut(s) 1605, 2550
Mva1269I GAATGC 3 cut(s) 1767, 2397, 3205
MvaI CCWGG 5 cut(s) 338, 517, 1356, 1697, 2325
MwoI GCNNNNNNNGC 6 cut(s) 110, 219, 330, 1246, 2970, 3171
NciI CCSGG 1 cut(s) 858
NlaIV GGNNCC 7 cut(s) 271, 1353, 1376, 1518, 1694, 1972, 2590
NmeAIII GCCGAG 2 cut(s) 2940, 2949
NmuCI GTSAC 3 cut(s) 232, 1571, 2368
NspI RCATGY 4 cut(s) 697, 2670, 2710, 2782
PaeR7I CTCGAG 3 cut(s) 449, 1149, 1481
PagI TCATGA 1 cut(s) 2658
PasI CCCWGGG 1 cut(s) 516
PceI AGGCCT 1 cut(s) 2229
PciI ACATGT 2 cut(s) 693, 2706
PctI GAATGC 3 cut(s) 1767, 2397, 3205
PdmI GAANNNNTTC 2 cut(s) 1839, 2397
PfeI GAWTC 6 cut(s) 259, 1454, 1534, 1828, 2170, 3273
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 2 cut(s) 2700, 2720
PleI GAGTC 5 cut(s) 239, 460, 1084, 2261, 2752
PpsI GAGTC 5 cut(s) 239, 460, 1084, 2261, 2752
PscI ACATGT 2 cut(s) 693, 2706
Psp6I CCWGG 5 cut(s) 336, 515, 1354, 1695, 2323
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 5 cut(s) 336, 515, 1354, 1695, 2323
PspN4I GGNNCC 7 cut(s) 271, 1353, 1376, 1518, 1694, 1972, 2590
PspOMI GGGCCC 1 cut(s) 1516
PspPI GGNCC 6 cut(s) 244, 1277, 1516, 1517, 2068, 2093
PspXI VCTCGAGB 2 cut(s) 449, 1481
PstI CTGCAG 2 cut(s) 1810, 3001
PsuI RGATCY 4 cut(s) 715, 816, 1702, 2021
RsaI GTAC 8 cut(s) 390, 1105, 1116, 1368, 1812, 2412, 2990, 3210
RsaNI GTAC 8 cut(s) 389, 1104, 1115, 1367, 1811, 2411, 2989, 3209
RseI CAYNNNNRTG 1 cut(s) 2363
SalI GTCGAC 1 cut(s) 1922
SatI GCNGC 2 cut(s) 2699, 2719
Sau96I GGNCC 6 cut(s) 244, 1277, 1516, 1517, 2068, 2093
ScaI AGTACT 1 cut(s) 1105
SchI GAGTC 5 cut(s) 240, 461, 1085, 2262, 2752
ScrFI CCNGG 6 cut(s) 338, 517, 858, 1356, 1697, 2325
SduI GDGCHC 5 cut(s) 1520, 1665, 2844, 3297, 3322
SfaNI GCATC 6 cut(s) 379, 1024, 2342, 2596, 2929, 3148
SfcI CTRYAG 3 cut(s) 711, 1806, 2997
Sfr274I CTCGAG 3 cut(s) 449, 1149, 1481
SinI GGWCC 3 cut(s) 244, 1277, 2068
SlaI CTCGAG 3 cut(s) 449, 1149, 1481
SmiMI CAYNNNNRTG 1 cut(s) 2363
SseBI AGGCCT 1 cut(s) 2229
SsiI CCGC 6 cut(s) 322, 1690, 2030, 2626, 3012, 3339
SspI AATATT 2 cut(s) 1582, 1867
SspMI CTAG 8 cut(s) 665, 1436, 1712, 2178, 2859, 2952, 3189, 3323
StuI AGGCCT 1 cut(s) 2229
StyD4I CCNGG 6 cut(s) 336, 515, 856, 1354, 1695, 2323
StyI CCWWGG 3 cut(s) 240, 265, 1294
TaaI ACNGT 9 cut(s) 151, 970, 1184, 1366, 2418, 2846, 2993, 3140, 3208
TaiI ACGT 3 cut(s) 657, 2898, 2979
TaqI TCGA 9 cut(s) 450, 1150, 1314, 1482, 1923, 2380, 2787, 2874, 3147
TatI WGTACW 6 cut(s) 388, 1103, 1114, 1366, 2410, 3208
TfiI GAWTC 6 cut(s) 259, 1454, 1534, 1828, 2170, 3273
TscAI CASTG 8 cut(s) 97, 1410, 1821, 1879, 2206, 2802, 2849, 3145
TseFI GTSAC 3 cut(s) 232, 1571, 2368
TseI GCWGC 2 cut(s) 2698, 2718
Tsp45I GTSAC 3 cut(s) 232, 1571, 2368
TspGWI ACGGA 2 cut(s) 2450, 3092
TspRI CASTG 8 cut(s) 97, 1410, 1821, 1879, 2206, 2802, 2849, 3145
Vha464I CTTAAG 3 cut(s) 59, 1099, 1567
VneI GTGCAC 2 cut(s) 2840, 3318
VpaK11BI GGWCC 3 cut(s) 244, 1277, 2068
XbaI TCTAGA 1 cut(s) 1435
XceI RCATGY 4 cut(s) 697, 2670, 2710, 2782
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 1821
XhoI CTCGAG 3 cut(s) 449, 1149, 1481
XmiI GTMKAC 2 cut(s) 1923, 2523
XmnI GAANNNNTTC 2 cut(s) 1839, 2397
XspI CTAG 8 cut(s) 665, 1436, 1712, 2178, 2859, 2952, 3189, 3323
ZrmI AGTACT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.