Rroxscaffold_1G00041140

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000058
Physical Location & Seq
Forward (+)
58904884 .. 58908956
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041140.1

Sequence Viewer

Length: 3174 bp
ATGGAGAGAACTTGGTTCTTCCTGGTATGCTGCTTTCCTATAGCGGTAGCAGCACATACCAATAGTAATCTCACCACAGACCAGTCTGCTCTTCTTGCTCTCAAAGCTCGTGTCACCGGTGACCCTAACATGATCTTGACCAACTGGTCTACCAACACACCTACTTGCACCTGGGTTGGCGTTACTTGTGCCACAAACCACTTCAGAGTTGCAGTCTTGAACCTCTCTTACATGGGACTCACAGGCACCATTCCTCCAGATCTTGGCAACCTCTCATTTCTTGTTGAGCTGCGTTTCGCAAATAACAGTTTTCATGGTTCCTTGCCTGAAGAATTGGCTCGTTTGCACCGGTTGAAGTTGATTGGCTTAGCATACAACAGCTTTAGCGGAGTCATTCCATCATGGTTCGGGTCCTTGTCCAAACTTCAAACGTTCAATTTGACTGGTAATCAGTTTTCAGGTTCCATACCCTCTGCCATTTTCAACTTATCTGCACTGCAAGTCATTAATTTGAGCCATAACCAACTATCAGGTAGCATACCAAGAGAAATTGGAAACCTAACCATGCTGAAGAGAATATTTCTTGATGATAATAAGTTCACAGAAATTCCAAATGAGATTGGAACTTTAAATCAACTCGAGATGTTATATGTGCAGCTCAATTCCCTATTGGGGCATGTTCCCATAGGTATCTTCAACATATCTTCTTTGACTACTTTGACTTTTTATGGAAACAACTTGAGCGGTAGTCTTTCAGATAACATATGCCAACATCTTCCTAGCATTCAAGTGTTGGATTTTTCTTACAACCAGTTTCATGGTCCACTTCCATCCAAATGGTGGCAATGCAAACGACTTCTTCAGTTAGGATTGTCGAATAACAATTTCAGTGGAAGTATACCTAACAATATTGGGAACTTAACCCAAATAAAGGCAATATACCTGGACATAAATCATTTGACAGGTACTATTCCACATGAGATTGGTCAGCTTCCATATTTGGAGGAATTGTCCCTCGGTATTAATAATCTCAAAGGGCTCATTCCATCCTCAATCTTCAATATGTCCACAATAATGAAGATATCGCTTAGCAACAATCAGCTCTCAGGTAGCCTCCCAGCAAACATAGGTCATTGGATTCCAATCCTTCAAGAGCTATACCTAGGAATAAATAAACTTAGCGGAGTAATCCCCAAATCCATCTCCAATGCTTCTAAGCTCACGGATCTAGACCTAGACCGTAACTTTTTTTTTGGCTTTATTCCAAGCACACTGTGTGTTTTAAAGAACCTTGAACAACTTAAGTTATTCCATAATAACTTGACCATTGATAGTTCGACTCCAGAAGTAAATATTCTCTCTTGTTTGGCAAATCTTAAGAATTTACAGGAATTAGCCTTCTCAACCAACCCATTAAATGTCAGGCTTCCAGTTTCCTTTAAGAATCTCTCTACATCACTCCAATATATTTATTTAAGCAATTGCAACATGAGAGGTAACATCCCCAATGATATAGGCAACTTGAGCAGCTTGATAGTCTTGAACTTGGCAGAAAATCAATTGAGTGGATTGATTCCAACCTCAGTAAGAAGACTATATAATCTCCAAGGTTTGTATTTATATGACAACAGATTGCAAGGATATATCCCGAATGAAGTTTGTCAACTAAACTACCTAGCTGAGTTAATTTTGTATGGGAATCAACTCACTGGTTGCATACCTTCTTGCTTGGGTAATCTAACTGCAGCGCTAAGAATTCTATCAATAGGGTCCAATTTGCTGAATTCAACAATACCATCGACCTTGTGGGATCTTACTGATATCTTGCAAGTAAATTTGTCTTCAAATTCTCTGAGTGGATCTCTCTCACAAGGTGTTGGTAATTTGAAAGTTGCCACAGATATATTGTTATCGTACAACCAATTGTCAGGCACTATACCAAGCAACATTGGGGGTCTTCAAGATTTGGTGAATCTCGACTTGGGAAATAATAAACTAGAAGGCTCAATTCCTAGTTCACTTGGCAACTCTTTAAGCCTGAAGTTCTTGGATTTATCCAAAAACCAATTATTTGGAGTGATTCCGAAGTCTCTTGAAGCACTCTCATATCTCCAGTATCTGAATTTGTCTTTTAACAAACTCCAAGGTGAAATTCCAACGGATGGACCTTTCAGAAACTTCTCTGCTCAATCATTTGTTTCTAATCATGGACGTCTCTGTGGGCCATCTCGGTTTCATGTTCCACCATGCAAAAAAAGAAGTGGTAGATCTATCTTGAAATATGTCATTCCAGGGATCTTGTCAACAATGCTTATATTGACCTCCATTTGGATGTTGATGTTACACAGAAAAAAGAATGTGAAATCTGCAACTGGAACTACCTTGCCTCAACTTCTATGGAGAAGAGTTTCATACCAAGAGCTTCTAAGTACAACAAATGGATTCCATGAAGGTAACTTACTTGGCACTGGGGGTTTTGGCTCAGTATATGCAGGAACACTTTCAGATGGGATAGACGTTGCCATAAAAGTTTTCAATTTAGAGCTAGAAGGAGCATCCACGAGTTTTGATGTTGAATGTGAGATGTTAAGCAATATTCGTCACCGAAATCTTATCAAAGTCATAAGTTGCTGCAGTCAAATTGATTTCAAAGCGGTTGTACTGAACTATATGCCTAATGGGAGCCTTGACAAGTGGTTATATTCTCCGAACTATTCCTTGAATATCCTGCAGAGGTTGAATATATTGATAGATGTTGCATCGGCATTGGAGTATCTTCATCATGGTTATGAAACACCTATTGTGCATTGTGATTTGAAGCCCAGAAACATATTACTAGACAATGATATGGGTGCACATGTTACTGACTTTGGTATTGCAAAACTCTTGGGCGGAGGAGATTCTATGACTCGCACCATGACCCTAGCCACAATTGGTTATATGGCTCCAGTTGATGTCGTGGACACCGATTTACTTCAGACAGAGGAAGATGATGATATTGTGAGCAAGAGAGATTGTTTGTCATCCCTTATGCAATTGGCTCTTTCTTGTTCTGCAGAATCATCAGAAGAGAGAATAAACATGCAAGAGGCTTTAGCCACCCTTAACAAAATCAAGATCAAGTTTTTGAAAAACGCTGCAGCAGTGGTGTTGAACCCTAGAGCTGAGATTTGA

Protein Analysis

1057

Amino Acids

116.21

Weight (kDa)

6.72

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 63 3.1e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 117 - 176 3e-10 Leucine rich repeat
LRR_8 PF13855 158 - 200 4.2e-06 Leucine rich repeat
LRR_14 PF23598 160 - 245 6.7e-09 Leucine-rich repeat region
LRR_14 PF23598 284 - 467 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 383 - 441 1.2e-06 Leucine rich repeat
LRR_14 PF23598 486 - 592 8.7e-08 Leucine-rich repeat region
LRR_8 PF13855 510 - 569 3.2e-06 Leucine rich repeat
Pkinase PF00069 815 - 984 2.3e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 818 - 983 2e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 145
AatII GACGTC 1 cut(s) 2215
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 3 cut(s) 263, 840, 2162
AccBSI CCGCTC 1 cut(s) 744
AccI GTMKAC 2 cut(s) 149, 898
AciI CCGC 6 cut(s) 44, 387, 744, 1182, 2654, 2892
AclI AACGTT 1 cut(s) 431
AclWI GGATC 4 cut(s) 1233, 1818, 1867, 2303
AcsI RAATTY 8 cut(s) 606, 1381, 1755, 1783, 1834, 1846, 2122, 2151
AcuI CTGAAG 6 cut(s) 187, 348, 590, 845, 2060, 2960
AcyI GRCGYC 1 cut(s) 2212
AdeI CACNNNGTG 3 cut(s) 1275, 1277, 1874
AfaI GTAC 4 cut(s) 967, 1916, 2431, 2661
AfeI AGCGCT 1 cut(s) 1749
AfiI CCNNNNNNNGG 6 cut(s) 263, 672, 840, 931, 2162, 2328
AflII CTTAAG 2 cut(s) 1301, 1376
AflIII ACRYGT 1 cut(s) 2857
AgeI ACCGGT 2 cut(s) 116, 348
AjnI CCWGG 4 cut(s) 21, 170, 942, 2290
AjuI GAANNNNNNNTTGG 4 cut(s) 1964, 1996, 2136, 2168
Alw21I GWGCWC 1 cut(s) 2857
Alw26I GTCTC 2 cut(s) 2094, 2219
Alw44I GTGCAC 1 cut(s) 2853
AlwI GGATC 4 cut(s) 1233, 1818, 1867, 2303
AlwNI CAGNNNCTG 1 cut(s) 2119
Ama87I CYCGRG 1 cut(s) 638
Aor51HI AGCGCT 1 cut(s) 1749
AoxI GGCC 1 cut(s) 2222
ApaLI GTGCAC 1 cut(s) 2853
ApeKI GCWGC 9 cut(s) 30, 50, 289, 655, 1527, 1745, 2631, 3137, 3140
ApoI RAATTY 8 cut(s) 606, 1381, 1755, 1783, 1834, 1846, 2122, 2151
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AseI ATTAAT 2 cut(s) 507, 1023
AsiGI ACCGGT 2 cut(s) 116, 348
Asp700I GAANNNNTTC 2 cut(s) 2407, 2500
AspA2I CCTAGG 1 cut(s) 1162
AspLEI GCGC 1 cut(s) 1750
AspS9I GGNCC 5 cut(s) 411, 821, 1770, 2165, 2222
AsuHPI GGTGA 6 cut(s) 64, 106, 131, 1981, 2159, 2594
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 4 cut(s) 411, 821, 1770, 2165
AvrII CCTAGG 1 cut(s) 1162
BaeGI GKGCMC 1 cut(s) 2857
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 1041
BarI GAAGNNNNNNTAC 2 cut(s) 2442, 2474
BauI CACGAG 2 cut(s) 108, 2560
BbsI GAAGAC 3 cut(s) 1597, 1833, 1949
Bbv12I GWGCWC 1 cut(s) 2857
BbvI GCAGC 9 cut(s) 17, 62, 276, 667, 1539, 1757, 2618, 3124, 3152
BccI CCATC 8 cut(s) 406, 838, 1054, 1208, 1804, 2156, 2233, 2501
BciT130I CCWGG 4 cut(s) 23, 172, 944, 2292
BcoDI GTCTC 2 cut(s) 2094, 2219
BfmI CTRYAG 6 cut(s) 39, 1743, 2632, 2729, 3054, 3138
BfoI RGCGCY 1 cut(s) 1751
BfrI CTTAAG 2 cut(s) 1301, 1376
BglII AGATCT 2 cut(s) 259, 2267
BisI GCNGC 9 cut(s) 31, 51, 290, 656, 1528, 1746, 2632, 3138, 3141
BlnI CCTAGG 1 cut(s) 1162
BlpI GCTNAGC 2 cut(s) 367, 1088
BlsI GCNGC 9 cut(s) 32, 52, 291, 657, 1529, 1747, 2633, 3139, 3142
Bme1390I CCNGG 4 cut(s) 23, 172, 944, 2292
Bme18I GGWCC 4 cut(s) 411, 821, 1770, 2165
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 5 cut(s) 411, 821, 1770, 2165, 2222
BmiI GGNNCC 7 cut(s) 247, 319, 412, 463, 1771, 2684, 2946
BmrFI CCNGG 4 cut(s) 23, 172, 944, 2292
BmrI ACTGGG 1 cut(s) 2478
BmsI GCATC 2 cut(s) 2564, 2768
BmuI ACTGGG 1 cut(s) 2478
BpiI GAAGAC 3 cut(s) 1597, 1833, 1949
BplI GAGNNNNNCTC 2 cut(s) 1846, 1878
BpmI CTGGAG 4 cut(s) 240, 1326, 2096, 2931
Bpu1102I GCTNAGC 2 cut(s) 367, 1088
BpuEI CTTGAG 2 cut(s) 760, 1543
BsaBI GATNNNNATC 1 cut(s) 1142
BsaHI GRCGYC 1 cut(s) 2212
BsaJI CCNNGG 6 cut(s) 171, 1015, 1162, 1606, 2143, 2291
BsaWI WCCGGW 2 cut(s) 116, 348
BsaXI ACNNNNNCTCC 4 cut(s) 238, 268, 995, 1025
Bsc4I CCNNNNNNNGG 6 cut(s) 263, 672, 840, 931, 2162, 2328
Bse118I RCCGGY 2 cut(s) 116, 348
Bse3DI GCAATG 1 cut(s) 851
Bse8I GATNNNNATC 1 cut(s) 1142
BseBI CCWGG 4 cut(s) 23, 172, 944, 2292
BseDI CCNNGG 6 cut(s) 171, 1015, 1162, 1606, 2143, 2291
BseGI GGATG 7 cut(s) 830, 1046, 1500, 2167, 2337, 2555, 3023
BseJI GATNNNNATC 1 cut(s) 1142
BseLI CCNNNNNNNGG 6 cut(s) 263, 672, 840, 931, 2162, 2328
BseMI GCAATG 1 cut(s) 851
BseMII CTCAG 6 cut(s) 1119, 1596, 1671, 1844, 2496, 3156
BseRI GAGGAG 1 cut(s) 2910
BseSI GKGCMC 1 cut(s) 2857
BseXI GCAGC 9 cut(s) 17, 62, 276, 667, 1539, 1757, 2618, 3124, 3152
BseYI CCCAGC 1 cut(s) 1117
BsgI GTGCAG 2 cut(s) 477, 674
BshFI GGCC 1 cut(s) 2224
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 2 cut(s) 116, 348
BsiHKAI GWGCWC 1 cut(s) 2857
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 2 cut(s) 117, 349
BslFI GGGAC 2 cut(s) 249, 997
BslI CCNNNNNNNGG 6 cut(s) 263, 672, 840, 931, 2162, 2328
BsmAI GTCTC 2 cut(s) 2094, 2219
BsmBI CGTCTC 1 cut(s) 2219
BsmFI GGGAC 2 cut(s) 249, 997
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 1 cut(s) 2224
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 2 cut(s) 1041, 2857
Bsp143I GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
Bsp1720I GCTNAGC 2 cut(s) 367, 1088
BspACI CCGC 6 cut(s) 44, 387, 744, 1182, 2654, 2892
BspANI GGCC 1 cut(s) 2224
BspCNI CTCAG 6 cut(s) 1118, 1595, 1672, 1845, 2495, 3157
BspLI GGNNCC 7 cut(s) 247, 319, 412, 463, 1771, 2684, 2946
BspMAI CTGCAG 5 cut(s) 1747, 2636, 2733, 3058, 3142
BspPI GGATC 4 cut(s) 1233, 1818, 1867, 2303
BspQI GCTCTTC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 2 cut(s) 1301, 1376
BsrBI CCGCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 851
BsrFI RCCGGY 2 cut(s) 116, 348
BssAI RCCGGY 2 cut(s) 116, 348
BssECI CCNNGG 6 cut(s) 171, 1015, 1162, 1606, 2143, 2291
BssMI GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
BssNAI GTATAC 1 cut(s) 899
BssNI GRCGYC 1 cut(s) 2212
BssSI CACGAG 2 cut(s) 108, 2560
BssT1I CCWWGG 3 cut(s) 1162, 1606, 2143
Bst1107I GTATAC 1 cut(s) 899
Bst2BI CACGAG 2 cut(s) 108, 2560
Bst2UI CCWGG 4 cut(s) 23, 172, 944, 2292
Bst4CI ACNGT 3 cut(s) 308, 1241, 1275
Bst6I CTCTTC 4 cut(s) 96, 566, 2398, 3063
BstACI GRCGYC 1 cut(s) 2212
BstAFI CTTAAG 2 cut(s) 1301, 1376
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 7 cut(s) 830, 1046, 1500, 2167, 2337, 2555, 3023
BstH2I RGCGCY 1 cut(s) 1751
BstHHI GCGC 1 cut(s) 1750
BstKTI GATC 8 cut(s) 135, 262, 1228, 1813, 1862, 2270, 2298, 3120
BstMAI GTCTC 2 cut(s) 2094, 2219
BstMBI GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
BstMWI GCNNNNNNNGC 4 cut(s) 50, 95, 104, 1524
BstNI CCWGG 4 cut(s) 23, 172, 944, 2292
BstNSI RCATGY 3 cut(s) 680, 2861, 3085
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 4 cut(s) 21, 170, 942, 2290
BstSFI CTRYAG 6 cut(s) 39, 1743, 2632, 2729, 3054, 3138
BstSLI GKGCMC 1 cut(s) 2857
BstV1I GCAGC 9 cut(s) 17, 62, 276, 667, 1539, 1757, 2618, 3124, 3152
BstV2I GAAGAC 3 cut(s) 1597, 1833, 1949
BstX2I RGATCY 6 cut(s) 259, 1225, 1810, 1859, 2267, 2295
BstXI CCANNNNNNTGG 3 cut(s) 818, 837, 2072
BstYI RGATCY 6 cut(s) 259, 1225, 1810, 1859, 2267, 2295
BstZ17I GTATAC 1 cut(s) 899
BsuRI GGCC 1 cut(s) 2224
BtsCI GGATG 7 cut(s) 830, 1046, 1500, 2167, 2337, 2555, 3023
BtsI GCAGTG 2 cut(s) 494, 3150
BtsIMutI CAGTG 6 cut(s) 494, 895, 1271, 1707, 2466, 3150
CaiI CAGNNNCTG 1 cut(s) 2119
CfoI GCGC 1 cut(s) 1750
Cfr10I RCCGGY 2 cut(s) 116, 348
Cfr13I GGNCC 5 cut(s) 411, 821, 1770, 2165, 2222
Csp6I GTAC 4 cut(s) 966, 1915, 2430, 2660
CspAI ACCGGT 2 cut(s) 116, 348
CspCI CAANNNNNGTGG 4 cut(s) 871, 906, 2548, 2583
CviQI GTAC 4 cut(s) 966, 1915, 2430, 2660
DpnI GATC 8 cut(s) 134, 261, 1227, 1812, 1861, 2269, 2297, 3119
DpnII GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
DraI TTTAAA 2 cut(s) 630, 1284
DraIII CACNNNGTG 3 cut(s) 1275, 1277, 1874
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
Eam1104I CTCTTC 4 cut(s) 96, 566, 2398, 3063
EarI CTCTTC 4 cut(s) 96, 566, 2398, 3063
EciI GGCGGA 1 cut(s) 2907
Eco130I CCWWGG 3 cut(s) 1162, 1606, 2143
Eco24I GRGCYC 1 cut(s) 1041
Eco32I GATATC 2 cut(s) 1083, 1822
Eco47I GGWCC 4 cut(s) 411, 821, 1770, 2165
Eco47III AGCGCT 1 cut(s) 1749
Eco57I CTGAAG 6 cut(s) 187, 348, 590, 845, 2060, 2960
Eco88I CYCGRG 1 cut(s) 638
Eco91I GGTNACC 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 411
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 2 cut(s) 1755, 1783
EcoRII CCWGG 4 cut(s) 21, 170, 942, 2290
EcoRV GATATC 2 cut(s) 1083, 1822
EcoT14I CCWWGG 3 cut(s) 1162, 1606, 2143
EcoT38I GRGCYC 1 cut(s) 1041
ErhI CCWWGG 3 cut(s) 1162, 1606, 2143
Esp3I CGTCTC 1 cut(s) 2219
FaqI GGGAC 2 cut(s) 249, 997
FauNDI CATATG 1 cut(s) 764
FblI GTMKAC 2 cut(s) 149, 898
Fnu4HI GCNGC 9 cut(s) 31, 51, 290, 656, 1528, 1746, 2632, 3138, 3141
FokI GGATG 7 cut(s) 817, 1033, 1487, 2174, 2344, 2542, 3010
FriOI GRGCYC 1 cut(s) 1041
Fsp4HI GCNGC 9 cut(s) 31, 51, 290, 656, 1528, 1746, 2632, 3138, 3141
GlaI GCGC 1 cut(s) 1749
GluI GCNGC 9 cut(s) 31, 51, 290, 656, 1528, 1746, 2632, 3138, 3141
GsaI CCCAGC 1 cut(s) 1121
GsuI CTGGAG 4 cut(s) 240, 1326, 2096, 2931
HaeII RGCGCY 1 cut(s) 1751
HaeIII GGCC 1 cut(s) 2224
HapII CCGG 2 cut(s) 117, 349
HhaI GCGC 1 cut(s) 1750
Hin1I GRCGYC 1 cut(s) 2212
Hin6I GCGC 1 cut(s) 1748
HinP1I GCGC 1 cut(s) 1748
HincII GTYRAC 2 cut(s) 1664, 2304
HindII GTYRAC 2 cut(s) 1664, 2304
HpaII CCGG 2 cut(s) 117, 349
HphI GGTGA 6 cut(s) 64, 106, 131, 1981, 2159, 2594
HpyAV CCTTC 6 cut(s) 1157, 1408, 1731, 1994, 2444, 2543
HpyCH4III ACNGT 3 cut(s) 308, 1241, 1275
HpyCH4IV ACGT 3 cut(s) 431, 2212, 2517
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 95, 104, 1524
HpySE526I ACGT 3 cut(s) 431, 2212, 2517
Hsp92I GRCGYC 1 cut(s) 2212
HspAI GCGC 1 cut(s) 1748
Kzo9I GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 3 cut(s) 2552, 2682, 2950
Lsp1109I GCAGC 9 cut(s) 17, 62, 276, 667, 1539, 1757, 2618, 3124, 3152
LweI GCATC 2 cut(s) 2564, 2768
MaeII ACGT 3 cut(s) 431, 2212, 2517
MaeIII GTNAC 9 cut(s) 112, 119, 181, 1241, 1496, 2340, 2453, 2600, 2860
MalI GATC 8 cut(s) 134, 261, 1227, 1812, 1861, 2269, 2297, 3119
MbiI CCGCTC 1 cut(s) 744
MboI GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
MfeI CAATTG 5 cut(s) 1480, 1559, 1922, 2931, 3035
MflI RGATCY 6 cut(s) 259, 1225, 1810, 1859, 2267, 2295
MhlI GDGCHC 2 cut(s) 1041, 2857
MlyI GAGTC 4 cut(s) 231, 399, 1333, 2902
MmeI TCCRAC 3 cut(s) 774, 1601, 2180
MroXI GAANNNNTTC 2 cut(s) 2407, 2500
MslI CAYNNNNRTG 5 cut(s) 788, 835, 1073, 2330, 2787
MspCI CTTAAG 2 cut(s) 1301, 1376
MspI CCGG 2 cut(s) 117, 349
MspR9I CCNGG 4 cut(s) 23, 172, 944, 2292
MunI CAATTG 5 cut(s) 1480, 1559, 1922, 2931, 3035
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 4 cut(s) 23, 172, 944, 2292
MwoI GCNNNNNNNGC 4 cut(s) 50, 95, 104, 1524
NdeI CATATG 1 cut(s) 764
NdeII GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
NlaIV GGNNCC 7 cut(s) 247, 319, 412, 463, 1771, 2684, 2946
NmuCI GTSAC 3 cut(s) 112, 119, 2600
NspI RCATGY 3 cut(s) 680, 2861, 3085
PaeR7I CTCGAG 1 cut(s) 638
PciI ACATGT 1 cut(s) 2857
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 2964
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 2 cut(s) 2407, 2500
PfeI GAWTC 9 cut(s) 1138, 1444, 1573, 1699, 1972, 2080, 2442, 2900, 3059
PflMI CCANNNNNTGG 3 cut(s) 263, 840, 2162
PinAI ACCGGT 2 cut(s) 116, 348
PkrI GCNGC 9 cut(s) 32, 52, 291, 657, 1529, 1747, 2633, 3139, 3142
PleI GAGTC 4 cut(s) 231, 398, 1333, 2902
PpsI GAGTC 4 cut(s) 231, 398, 1333, 2902
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 1 cut(s) 2857
PshBI ATTAAT 2 cut(s) 507, 1023
Psp1406I AACGTT 1 cut(s) 431
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 4 cut(s) 21, 170, 942, 2290
PspEI GGTNACC 1 cut(s) 119
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 4 cut(s) 21, 170, 942, 2290
PspN4I GGNNCC 7 cut(s) 247, 319, 412, 463, 1771, 2684, 2946
PspPI GGNCC 5 cut(s) 411, 821, 1770, 2165, 2222
PspPPI RGGWCCY 1 cut(s) 411
PsrI GAACNNNNNNTAC 2 cut(s) 212, 244
PstI CTGCAG 5 cut(s) 1747, 2636, 2733, 3058, 3142
PstNI CAGNNNCTG 1 cut(s) 2119
PsuI RGATCY 6 cut(s) 259, 1225, 1810, 1859, 2267, 2295
RsaI GTAC 4 cut(s) 967, 1916, 2431, 2661
RsaNI GTAC 4 cut(s) 966, 1915, 2430, 2660
RseI CAYNNNNRTG 5 cut(s) 788, 835, 1073, 2330, 2787
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 9 cut(s) 31, 51, 290, 656, 1528, 1746, 2632, 3138, 3141
Sau3AI GATC 8 cut(s) 132, 259, 1225, 1810, 1859, 2267, 2295, 3117
Sau96I GGNCC 5 cut(s) 411, 821, 1770, 2165, 2222
SchI GAGTC 4 cut(s) 231, 399, 1333, 2902
ScrFI CCNGG 4 cut(s) 23, 172, 944, 2292
SduI GDGCHC 2 cut(s) 1041, 2857
SfaNI GCATC 2 cut(s) 2564, 2768
SfcI CTRYAG 6 cut(s) 39, 1743, 2632, 2729, 3054, 3138
Sfr274I CTCGAG 1 cut(s) 638
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 4 cut(s) 411, 821, 1770, 2165
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 5 cut(s) 788, 835, 1073, 2330, 2787
SmlI CTYRAG 5 cut(s) 638, 739, 1301, 1376, 1522
SmoI CTYRAG 5 cut(s) 638, 739, 1301, 1376, 1522
SsiI CCGC 6 cut(s) 44, 387, 744, 1182, 2654, 2892
SspI AATATT 4 cut(s) 579, 910, 1354, 2596
StyD4I CCNGG 4 cut(s) 21, 170, 942, 2290
StyI CCWWGG 3 cut(s) 1162, 1606, 2143
TaaI ACNGT 3 cut(s) 308, 1241, 1275
TaiI ACGT 3 cut(s) 434, 2215, 2520
TaqI TCGA 5 cut(s) 639, 875, 1337, 1799, 1977
TatI WGTACW 2 cut(s) 2429, 2659
TfiI GAWTC 9 cut(s) 1138, 1444, 1573, 1699, 1972, 2080, 2442, 2900, 3059
TscAI CASTG 6 cut(s) 501, 895, 1278, 1714, 2473, 3150
TseFI GTSAC 3 cut(s) 112, 119, 2600
TseI GCWGC 9 cut(s) 30, 50, 289, 655, 1527, 1745, 2631, 3137, 3140
Tsp45I GTSAC 3 cut(s) 112, 119, 2600
TspDTI ATGAA 9 cut(s) 302, 806, 1091, 1668, 2225, 2400, 2463, 2768, 2805
TspGWI ACGGA 2 cut(s) 1238, 2174
TspRI CASTG 6 cut(s) 501, 895, 1278, 1714, 2473, 3150
Van91I CCANNNNNTGG 3 cut(s) 263, 840, 2162
Vha464I CTTAAG 2 cut(s) 1301, 1376
VneI GTGCAC 1 cut(s) 2853
VpaK11BI GGWCC 4 cut(s) 411, 821, 1770, 2165
VspI ATTAAT 2 cut(s) 507, 1023
XapI RAATTY 8 cut(s) 606, 1381, 1755, 1783, 1834, 1846, 2122, 2151
XbaI TCTAGA 1 cut(s) 1228
XceI RCATGY 3 cut(s) 680, 2861, 3085
XcmI CCANNNNNNNNNTGG 2 cut(s) 837, 1803
XhoI CTCGAG 1 cut(s) 638
XmaJI CCTAGG 1 cut(s) 1162
XmiI GTMKAC 2 cut(s) 149, 898
XmnI GAANNNNTTC 2 cut(s) 2407, 2500
ZraI GACGTC 1 cut(s) 2213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.