Rw6G018950

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000058
Physical Location & Seq
Forward (+)
37741968 .. 37745870
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018950.1

Sequence Viewer

Length: 3354 bp
ATGGAGAGAATTGCTTGGTTCTTCCTGGTGCAGTACTGTTGTTGTATGGCGGCTACCTGCGTAACCATGGCAGCACATGTACAAACCAACATCAGCATAGACCAGTCTGCTCTTCTTGCTTTCAAAGCCAGTATCACCGATGATCCTCATAAACTGATCTTCACCAACTGGTCAACCATCGCCCCAGTTTGTAACTGGGTTGGCGTTACTTGTGCTGCTCGCCACCACAGAGTTGCGGTCTTGAACTTATCTTATTTTGGTCTCACTGGCACCATTCCTCCGGAGCTAGGAAACCTTTCATTTCTTGTGGAACTTCGCCTCAGAAATAACAGTTTTCATGGTACCTTGCCTCAGGAATTGGCTAGCCTGCACCGGTTGAGGTTGATTAGCTTGGGATATAACAAGTTTATGGGAACCATTCCCACATGGTTCGGGTCCTTATCCAAACTTCAAACATTTAATTTGTATGGTAATCAGTTTTCAGGTTCCATACCAGCAACCATCTTGAACTTATCTGCACTTCAAGTAATTGATCTGACGTATAACCAACTCTCAGGTAACATACCAAGAGAGATTGGAAACTTAACAATGTTGAAGGAAATATACCTGGACAGTAATAAATTCACAGAAATTCCTAATGAGATTGGCGGTTTAGATCAATTGGAAATATTGTTTGTGCAATTGAATGCTTTAAAAGGGTCTATTCCTATGGTTGTTTTCAACATGTCTTCTTTGACTATGTTGGCTCTATATGGAAACAATTTAAATGGTAGTCTTCCGGACAATATATGTCAACATCTTCCGAGTATTCAAGATTTGAGTCTGGGTTACAACCAATTCGACGGTCCACTTCCATCGAAATTGTGGGAATGCAAAGAACTCCTTCAATTGGAAGTACCACGCAACAGTTTCAGTGGAGAAATACCAAATACTTTTGGGAACTTGACTCAGATACAGACAATTGACCTTGGCTTGAACTATTTGACAGGTCCTATACCACATCAGATTGGTCATCTTCCAAATTTAGAGATATTGAACCTCGGTGGGAATAACTTCAATGAGGTCATCCCATCCTCAATCTTCAATATGTCCACAATAGCAGTGATATCGCTTTTCGAGAATCAGCTCTCAGGCAACCTTCCACCAAACACAGGCCTTGGCCTTCCAAACCTACAACGCTTCTATCTAGGAGGAAATAAACTTAGCGGAGCAATCCCCAAATCTTTGTCCAATGCTTCTAACCTGGAGCTCATAGACCTAGGCAGCAACCTATTATTTGGCGTTATTCCTAGCACCCTATGTACCTTGACAAAACTTCAGCTGCTTCAACTATTTGATAATAACCTGACCGTCAGTACTTCGACTCCAGAACTGGATGTTCTCTTTTGTTTGGCAAATCTTCGCAACTTGAGGAGACTAGACTTCTCAGTGAATCCATTAGATGCCAACCTTCCGATTTCCTTTACGAATACATCTCTTCAATATATTTATTTTTACAACTGCAACATGAGGGGTGAAATTCCCAATGACATCGGCAACTTGAGCAGCTTGATAGTATTATCTGTGCTAAAGAATCAATTAAGTGGATTGGTTCCAGCCTCAATAGGAAGAATCCGAAACCTCCAAGGTCTATATTTGGAAAGTAACACATTTCAAGGACGCATGCCAGATGAACTCTGCCAACTGAAAAACCTAGGTTCTTTGTTTTTGGGTGGCAATCGACTGTCTGGCTCTATACCTTCATGCATCGGCAATCTAACTGGAGCTCTAAGGAAACTATCATTGGAGTCTAATTTGTTAACTTCTACAGTACCATCTACCTTGTGGGGACTTACATATATCCTGTACTTGAACTTGTCATCGAATTCTCTGATCGGGTCTATCGGAGAAGGTGTTGGTAATTTGAAAGTTGCCACAGATATAGATTTTTCAAACAACCGATTATCCGGTAGTATAGCAAGCAGCGTTGGTGGACTTCAAAACTTGGTCAATCTGTCCTTGGCAAATAATAAACTACAAGGCTCCATTCCTACTTCACTTGGCAATTGCATAAGCCTGGAATCTTTGGACTTATCCAAAAACAACTTATCAGGAGTGATTCCCAACTCTCTTTCAGCGCTCTCATATCTGAAGTATTTGAATTTGTCTTTCAACAGACTCCAAGGAGAAATTCCAACAGGTGGTCCTTTCAGAAACTTGTCTACTCAATCATTTGTTTCAAACCATGGACTCTGTGGTGCAGCCCGATTTCAAGTTCCATCATGCAAAAGGAAGACTGTTAAAACTATTTTCAAGTATGTTATTCCGGGTATCTTGTCCGCAATGCTTCTAGTGACCTCCATTTGGATATTTATTAAATCGAGAAAGAAGAATGTGAAAGCTGCAACAGAGACTACCTTTTTGCCACAACATCAGTGGAGAAGAGTTTCCTATGAAGAGCTTCTTAGTGCAACAAGTGGATTCAATGTGAGCAACTTACTTGGCACTGGGAGTTTTGGCTCAGTATATAAAGGAACACTGTCAGATGGGATAGATGTTGCCATTAAGGTGTATAATTTAGACACAGAAGGGGCATTCTCAAGTTTTGATGTCGAATGCGAAATGCTAAGCAATATTCGCCATCGAAACCTTATCAAAGTCATCAGTTGCTGCAGTCAAGTTGATTTCAAAGCCGTTGTACTAAACTACATGCCTAATGGGAGCCTTGAAAAGTGGTTGTATTCTCATAACTTAACCTTGAACATCTTGCAGAGGTTGAATATATTGATAGATGTTGCAACGGCATTGGAGTATCTCCATCATGGTTATGATAGACCTATTGTGCATTGTGATCTGAAGCCAAGCAACATACTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGTATCGCAAAACTCTTGGGTGGAGAGGATTCTATCACTCGAACCTTGACCCTTGCCACAATCGGGTACATGGCTCCAGAGTATGGAATGGATGGAATAGTGACCAGAAGAGGGGATATGTATAGTTTTGGTGTTGTAGTGATGGAGACATTCACAAAAAGGAAGCCAACAGATGCGATGTTTGTGGGGGAAATGAATTTAAAGCACTGGGTTGCAAATTCACTTCTTGCAGATGCAATAGTTGAAGTTGTTGATACCACTTTACTTGGGACAGAGGAATATCATGACTTTGTGAGCAAGAGAGATTGTTTATCATCTATTATGAGATTGGGTATTGCTTGTTGTGGAGAATCACCAGAAGACAGGATAAATATGCACGAGGCTCTAGCCACGCTGAACAAAATCAAGATCAAATTTTTGAAGAACGCTTCAGAAGGTGTGGTATTGAGCCGTCATGGCCTCTGA

Protein Analysis

1117

Amino Acids

122.5

Weight (kDa)

6.61

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 71 1.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 181 7.7e-07 Leucine-rich repeat region
LRR_8 PF13855 125 - 184 2.1e-07 Leucine rich repeat
LRR_14 PF23598 168 - 277 8.3e-10 Leucine-rich repeat region
LRR_8 PF13855 297 - 352 1.3e-06 Leucine rich repeat
LRR_8 PF13855 341 - 401 2.3e-06 Leucine rich repeat
LRR_8 PF13855 389 - 449 3.7e-06 Leucine rich repeat
LRR_14 PF23598 455 - 595 1.4e-07 Leucine-rich repeat region
LRR_14 PF23598 590 - 720 8.3e-08 Leucine-rich repeat region
LRR_8 PF13855 661 - 720 1.2e-08 Leucine rich repeat
Pkinase PF00069 822 - 1093 8.9e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 823 - 1090 5.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 269, 341
AccB7I CCANNNNNTGG 2 cut(s) 2180, 2972
AccI GTMKAC 1 cut(s) 2201
AccIII TCCGGA 2 cut(s) 280, 778
AciI CCGC 5 cut(s) 50, 236, 648, 1206, 2319
AclWI GGATC 1 cut(s) 137
AcuI CTGAAG 4 cut(s) 1301, 2150, 2855, 3303
AfeI AGCGCT 1 cut(s) 2118
AfiI CCNNNNNNNGG 8 cut(s) 287, 889, 1151, 2180, 2343, 2952, 2972, 3000
AflIII ACRYGT 3 cut(s) 76, 723, 2875
AgeI ACCGGT 1 cut(s) 372
AjnI CCWGG 4 cut(s) 24, 606, 1242, 2055
AjuI GAANNNNNNNTTGG 4 cut(s) 1766, 1798, 2154, 2186
AluBI AGCT 9 cut(s) 286, 390, 1126, 1249, 1321, 1548, 1766, 2381, 2440
AluI AGCT 9 cut(s) 286, 390, 1126, 1249, 1321, 1548, 1766, 2381, 2440
Alw21I GWGCWC 2 cut(s) 1251, 1768
Alw26I GTCTC 4 cut(s) 266, 1408, 2384, 3029
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 2649
Aor13HI TCCGGA 2 cut(s) 280, 778
Aor51HI AGCGCT 1 cut(s) 2118
AoxI GGCC 3 cut(s) 1153, 1159, 3346
ApeKI GCWGC 9 cut(s) 71, 215, 1263, 1321, 1545, 1962, 2240, 2381, 2649
ArsI GACNNNNNNTTYG 2 cut(s) 2383, 2415
AsiGI ACCGGT 1 cut(s) 372
Asp700I GAANNNNTTC 5 cut(s) 295, 882, 1052, 2425, 2439
Asp718I GGTACC 1 cut(s) 341
AspA2I CCTAGG 2 cut(s) 1258, 1693
AspLEI GCGC 1 cut(s) 2119
AspS9I GGNCC 4 cut(s) 435, 845, 989, 2183
AsuC2I CCSGG 1 cut(s) 2307
AsuHPI GGTGA 4 cut(s) 127, 154, 1526, 3234
AsuNHI GCTAGC 1 cut(s) 362
AvaII GGWCC 4 cut(s) 435, 845, 989, 2183
AvrII CCTAGG 2 cut(s) 1258, 1693
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 2 cut(s) 269, 341
BanII GRGCYC 2 cut(s) 1251, 1768
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BauI CACGAG 1 cut(s) 3266
BbsI GAAGAC 4 cut(s) 720, 767, 2279, 3255
Bbv12I GWGCWC 2 cut(s) 1251, 1768
BbvI GCAGC 9 cut(s) 83, 202, 1275, 1308, 1557, 1974, 2252, 2368, 2636
BceAI ACGGC 3 cut(s) 2657, 2796, 3324
BcgI CGANNNNNNTGC 2 cut(s) 1089, 1123
BciT130I CCWGG 4 cut(s) 26, 608, 1244, 2057
BcnI CCSGG 1 cut(s) 2307
BcoDI GTCTC 4 cut(s) 266, 1408, 2384, 3029
BfmI CTRYAG 2 cut(s) 1806, 2650
BfoI RGCGCY 1 cut(s) 2120
BfuAI ACCTGC 1 cut(s) 65
BglI GCCNNNNNGGC 1 cut(s) 3345
BlnI CCTAGG 2 cut(s) 1258, 1693
BlpI GCTNAGC 1 cut(s) 2606
BmcAI AGTACT 2 cut(s) 35, 1357
Bme1390I CCNGG 5 cut(s) 26, 608, 1244, 2057, 2307
Bme18I GGWCC 4 cut(s) 435, 845, 989, 2183
BmgT120I GGNCC 4 cut(s) 435, 845, 989, 2183
BmiI GGNNCC 9 cut(s) 271, 343, 415, 436, 487, 1593, 2023, 2702, 2964
BmrFI CCNGG 5 cut(s) 26, 608, 1244, 2057, 2307
BmrI ACTGGG 4 cut(s) 179, 205, 2496, 3106
BmsI GCATC 4 cut(s) 1432, 1755, 3052, 3112
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 4 cut(s) 179, 205, 2496, 3106
BpiI GAAGAC 4 cut(s) 720, 767, 2279, 3255
BpmI CTGGAG 4 cut(s) 1265, 1350, 1782, 2949
Bpu1102I GCTNAGC 1 cut(s) 2606
BpuEI CTTGAG 3 cut(s) 1429, 1561, 2563
BpuMI CCSGG 1 cut(s) 2307
BsaI GGTCTC 1 cut(s) 266
BsaWI WCCGGW 4 cut(s) 280, 372, 778, 1946
BsaXI ACNNNNNCTCC 6 cut(s) 262, 292, 1348, 1378, 1878, 1908
Bsc4I CCNNNNNNNGG 8 cut(s) 287, 889, 1151, 2180, 2343, 2952, 2972, 3000
Bse118I RCCGGY 1 cut(s) 372
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 2328
BseAI TCCGGA 2 cut(s) 280, 778
BseBI CCWGG 4 cut(s) 26, 608, 1244, 2057
BseGI GGATG 4 cut(s) 1065, 1070, 1381, 2986
BseLI CCNNNNNNNGG 8 cut(s) 287, 889, 1151, 2180, 2343, 2952, 2972, 3000
BseMI GCAATG 1 cut(s) 2328
BseMII CTCAG 7 cut(s) 334, 365, 567, 962, 1143, 1440, 2514
BseRI GAGGAG 1 cut(s) 1426
BseXI GCAGC 9 cut(s) 83, 202, 1275, 1308, 1557, 1974, 2252, 2368, 2636
BsgI GTGCAG 4 cut(s) 50, 353, 501, 2259
BshFI GGCC 3 cut(s) 1155, 1161, 3348
BshNI GGYRCC 2 cut(s) 269, 341
BshTI ACCGGT 1 cut(s) 372
BsiHKAI GWGCWC 2 cut(s) 1251, 1768
BsiSI CCGG 5 cut(s) 281, 373, 779, 1947, 2306
BslFI GGGAC 2 cut(s) 1842, 3172
BslI CCNNNNNNNGG 8 cut(s) 287, 889, 1151, 2180, 2343, 2952, 2972, 3000
BsmAI GTCTC 4 cut(s) 266, 1408, 2384, 3029
BsmFI GGGAC 2 cut(s) 1842, 3172
BsmI GAATGC 4 cut(s) 691, 875, 2573, 2600
BsnI GGCC 3 cut(s) 1155, 1161, 3348
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 1251, 1768
Bsp13I TCCGGA 2 cut(s) 280, 778
Bsp1407I TGTACA 1 cut(s) 79
Bsp143I GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
Bsp1720I GCTNAGC 1 cut(s) 2606
Bsp19I CCATGG 2 cut(s) 66, 2224
BspACI CCGC 5 cut(s) 50, 236, 648, 1206, 2319
BspANI GGCC 3 cut(s) 1155, 1161, 3348
BspCNI CTCAG 7 cut(s) 333, 364, 566, 961, 1142, 1439, 2513
BspEI TCCGGA 2 cut(s) 280, 778
BspHI TCATGA 1 cut(s) 3172
BspLI GGNNCC 9 cut(s) 271, 343, 415, 436, 487, 1593, 2023, 2702, 2964
BspMAI CTGCAG 1 cut(s) 2654
BspMI ACCTGC 1 cut(s) 65
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 137
BspQI GCTCTTC 2 cut(s) 117, 2430
BspT107I GGYRCC 2 cut(s) 269, 341
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 2328
BsrFI RCCGGY 1 cut(s) 372
BsrGI TGTACA 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 372
BssMI GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
BssSI CACGAG 1 cut(s) 3266
BssT1I CCWWGG 9 cut(s) 66, 967, 1156, 1258, 1624, 1693, 1998, 2161, 2224
Bst2BI CACGAG 1 cut(s) 3266
Bst2UI CCWGG 4 cut(s) 26, 608, 1244, 2057
Bst6I CTCTTC 5 cut(s) 117, 1482, 2416, 2430, 2992
BstAPI GCANNNNNTGC 1 cut(s) 2879
BstAUI TGTACA 1 cut(s) 79
BstC8I GCNNGC 5 cut(s) 220, 364, 368, 1664, 1960
BstDSI CCRYGG 2 cut(s) 66, 2224
BstF5I GGATG 4 cut(s) 1065, 1070, 1381, 2986
BstH2I RGCGCY 1 cut(s) 2120
BstHHI GCGC 1 cut(s) 2119
BstKTI GATC 7 cut(s) 145, 159, 535, 658, 1875, 2833, 3300
BstMAI GTCTC 4 cut(s) 266, 1408, 2384, 3029
BstMBI GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
BstMWI GCNNNNNNNGC 6 cut(s) 116, 125, 1542, 2616, 2879, 3345
BstNI CCWGG 4 cut(s) 26, 608, 1244, 2057
BstNSI RCATGY 5 cut(s) 80, 727, 1666, 2692, 2879
BstSCI CCNGG 5 cut(s) 24, 606, 1242, 2055, 2305
BstSFI CTRYAG 2 cut(s) 1806, 2650
BstV1I GCAGC 9 cut(s) 83, 202, 1275, 1308, 1557, 1974, 2252, 2368, 2636
BstV2I GAAGAC 4 cut(s) 720, 767, 2279, 3255
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 3 cut(s) 1155, 1161, 3348
BtgI CCRYGG 2 cut(s) 66, 2224
BtgZI GCGATG 2 cut(s) 163, 3080
BtsCI GGATG 4 cut(s) 1065, 1070, 1381, 2986
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 8 cut(s) 264, 919, 1107, 1434, 2420, 2484, 2516, 3094
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 5 cut(s) 220, 364, 368, 1664, 1960
CaiI CAGNNNCTG 1 cut(s) 2649
CciI TCATGA 1 cut(s) 3172
CfoI GCGC 1 cut(s) 2119
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 4 cut(s) 435, 845, 989, 2183
CseI GACGC 1 cut(s) 1668
CspAI ACCGGT 1 cut(s) 372
CspCI CAANNNNNGTGG 4 cut(s) 2396, 2431, 3136, 3171
DpnI GATC 7 cut(s) 144, 158, 534, 657, 1874, 2832, 3299
DpnII GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
DraI TTTAAA 3 cut(s) 693, 765, 3090
Eam1104I CTCTTC 5 cut(s) 117, 1482, 2416, 2430, 2992
EarI CTCTTC 5 cut(s) 117, 1482, 2416, 2430, 2992
Ecl136II GAGCTC 2 cut(s) 1249, 1766
Eco130I CCWWGG 9 cut(s) 66, 967, 1156, 1258, 1624, 1693, 1998, 2161, 2224
Eco147I AGGCCT 1 cut(s) 1155
Eco24I GRGCYC 2 cut(s) 1251, 1768
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 4 cut(s) 435, 845, 989, 2183
Eco47III AGCGCT 1 cut(s) 2118
Eco53kI GAGCTC 2 cut(s) 1249, 1766
Eco57I CTGAAG 4 cut(s) 1301, 2150, 2855, 3303
Eco81I CCTNAGG 1 cut(s) 351
EcoICRI GAGCTC 2 cut(s) 1249, 1766
EcoO109I RGGNCCY 2 cut(s) 435, 989
EcoRI GAATTC 1 cut(s) 1864
EcoRII CCWGG 4 cut(s) 24, 606, 1242, 2055
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 9 cut(s) 66, 967, 1156, 1258, 1624, 1693, 1998, 2161, 2224
EcoT22I ATGCAT 1 cut(s) 1748
EcoT38I GRGCYC 2 cut(s) 1251, 1768
ErhI CCWWGG 9 cut(s) 66, 967, 1156, 1258, 1624, 1693, 1998, 2161, 2224
FalI AAGNNNNNCTT 4 cut(s) 867, 899, 2427, 2459
FaqI GGGAC 2 cut(s) 1842, 3172
FblI GTMKAC 1 cut(s) 2201
FokI GGATG 4 cut(s) 1052, 1057, 1388, 2993
FriOI GRGCYC 2 cut(s) 1251, 1768
GlaI GCGC 1 cut(s) 2118
GsuI CTGGAG 4 cut(s) 1265, 1350, 1782, 2949
HaeII RGCGCY 1 cut(s) 2120
HaeIII GGCC 3 cut(s) 1155, 1161, 3348
HapII CCGG 5 cut(s) 281, 373, 779, 1947, 2306
HgaI GACGC 1 cut(s) 1668
HhaI GCGC 1 cut(s) 2119
Hin6I GCGC 1 cut(s) 2117
HinP1I GCGC 1 cut(s) 2117
HincII GTYRAC 3 cut(s) 174, 794, 1800
HindII GTYRAC 3 cut(s) 174, 794, 1800
HpaI GTTAAC 1 cut(s) 1800
HpaII CCGG 5 cut(s) 281, 373, 779, 1947, 2306
HphI GGTGA 4 cut(s) 127, 154, 1526, 3234
Hpy166II GTNNAC 7 cut(s) 174, 794, 848, 1092, 1800, 1973, 2202
Hpy8I GTNNAC 7 cut(s) 174, 794, 848, 1092, 1800, 1973, 2202
Hpy99I CGWCG 1 cut(s) 845
HpyAV CCTTC 9 cut(s) 589, 893, 1148, 1172, 1460, 1749, 1883, 2561, 3317
HpyCH4IV ACGT 1 cut(s) 539
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 125, 1542, 2616, 2879, 3345
HpySE526I ACGT 1 cut(s) 539
HspAI GCGC 1 cut(s) 2117
Kpn2I TCCGGA 2 cut(s) 280, 778
KpnI GGTACC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 1800
Kzo9I GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
LguI GCTCTTC 2 cut(s) 117, 2430
LmnI GCTCC 7 cut(s) 283, 1208, 1246, 1763, 2027, 2700, 2968
Lsp1109I GCAGC 9 cut(s) 83, 202, 1275, 1308, 1557, 1974, 2252, 2368, 2636
LweI GCATC 4 cut(s) 1432, 1755, 3052, 3112
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 8 cut(s) 61, 191, 205, 557, 827, 1643, 2332, 2989
MalI GATC 7 cut(s) 144, 158, 534, 657, 1874, 2832, 3299
MboI GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
MfeI CAATTG 5 cut(s) 659, 680, 887, 960, 2044
MhlI GDGCHC 2 cut(s) 1251, 1768
MlyI GAGTC 6 cut(s) 829, 940, 1357, 1796, 2151, 2223
MmeI TCCRAC 1 cut(s) 2198
Mph1103I ATGCAT 1 cut(s) 1748
MroI TCCGGA 2 cut(s) 280, 778
MroXI GAANNNNTTC 5 cut(s) 295, 882, 1052, 2425, 2439
MslI CAYNNNNRTG 2 cut(s) 2546, 2805
MspA1I CMGCKG 1 cut(s) 1321
MspI CCGG 5 cut(s) 281, 373, 779, 1947, 2306
MspR9I CCNGG 5 cut(s) 26, 608, 1244, 2057, 2307
MunI CAATTG 5 cut(s) 659, 680, 887, 960, 2044
Mva1269I GAATGC 4 cut(s) 691, 875, 2573, 2600
MvaI CCWGG 4 cut(s) 26, 608, 1244, 2057
MwoI GCNNNNNNNGC 6 cut(s) 116, 125, 1542, 2616, 2879, 3345
NciI CCSGG 1 cut(s) 2307
NcoI CCATGG 2 cut(s) 66, 2224
NdeII GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
NheI GCTAGC 1 cut(s) 362
NlaIV GGNNCC 9 cut(s) 271, 343, 415, 436, 487, 1593, 2023, 2702, 2964
NmuCI GTSAC 2 cut(s) 2332, 2989
NsiI ATGCAT 1 cut(s) 1748
NspI RCATGY 5 cut(s) 80, 727, 1666, 2692, 2879
PaeI GCATGC 1 cut(s) 1666
PagI TCATGA 1 cut(s) 3172
PceI AGGCCT 1 cut(s) 1155
PciI ACATGT 3 cut(s) 76, 723, 2875
PciSI GCTCTTC 2 cut(s) 117, 2430
PctI GAATGC 4 cut(s) 691, 875, 2573, 2600
PdmI GAANNNNTTC 5 cut(s) 295, 882, 1052, 2425, 2439
PfeI GAWTC 9 cut(s) 1120, 1432, 1573, 1611, 2060, 2098, 2460, 2918, 3239
PflMI CCANNNNNTGG 2 cut(s) 2180, 2972
PinAI ACCGGT 1 cut(s) 372
PleI GAGTC 6 cut(s) 828, 940, 1357, 1795, 2151, 2223
PpsI GAGTC 6 cut(s) 828, 940, 1357, 1795, 2151, 2223
PpuMI RGGWCCY 2 cut(s) 435, 989
PscI ACATGT 3 cut(s) 76, 723, 2875
Psp124BI GAGCTC 2 cut(s) 1251, 1768
Psp5II RGGWCCY 2 cut(s) 435, 989
Psp6I CCWGG 4 cut(s) 24, 606, 1242, 2055
PspGI CCWGG 4 cut(s) 24, 606, 1242, 2055
PspN4I GGNNCC 9 cut(s) 271, 343, 415, 436, 487, 1593, 2023, 2702, 2964
PspPI GGNCC 4 cut(s) 435, 845, 989, 2183
PspPPI RGGWCCY 2 cut(s) 435, 989
PstI CTGCAG 1 cut(s) 2654
PstNI CAGNNNCTG 1 cut(s) 2649
PvuII CAGCTG 1 cut(s) 1321
RseI CAYNNNNRTG 2 cut(s) 2546, 2805
SacI GAGCTC 2 cut(s) 1251, 1768
SapI GCTCTTC 2 cut(s) 117, 2430
Sau3AI GATC 7 cut(s) 142, 156, 532, 655, 1872, 2830, 3297
Sau96I GGNCC 4 cut(s) 435, 845, 989, 2183
ScaI AGTACT 2 cut(s) 35, 1357
SchI GAGTC 6 cut(s) 829, 940, 1357, 1796, 2151, 2223
ScrFI CCNGG 5 cut(s) 26, 608, 1244, 2057, 2307
SduI GDGCHC 2 cut(s) 1251, 1768
SfaNI GCATC 4 cut(s) 1432, 1755, 3052, 3112
SfcI CTRYAG 2 cut(s) 1806, 2650
SinI GGWCC 4 cut(s) 435, 845, 989, 2183
SmiI ATTTAAAT 1 cut(s) 765
SmiMI CAYNNNNRTG 2 cut(s) 2546, 2805
SmlI CTYRAG 3 cut(s) 1408, 1540, 2578
SmoI CTYRAG 3 cut(s) 1408, 1540, 2578
SphI GCATGC 1 cut(s) 1666
SseBI AGGCCT 1 cut(s) 1155
SsiI CCGC 5 cut(s) 50, 236, 648, 1206, 2319
SspI AATATT 2 cut(s) 669, 2614
SstI GAGCTC 2 cut(s) 1251, 1768
StuI AGGCCT 1 cut(s) 1155
StyD4I CCNGG 5 cut(s) 24, 606, 1242, 2055, 2305
StyI CCWWGG 9 cut(s) 66, 967, 1156, 1258, 1624, 1693, 1998, 2161, 2224
SwaI ATTTAAAT 1 cut(s) 765
TaiI ACGT 1 cut(s) 542
TatI WGTACW 5 cut(s) 33, 79, 1355, 1845, 2677
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 9 cut(s) 1120, 1432, 1573, 1611, 2060, 2098, 2460, 2918, 3239
TscAI CASTG 8 cut(s) 271, 919, 1107, 1434, 2420, 2491, 2523, 3101
TseFI GTSAC 2 cut(s) 2332, 2989
TseI GCWGC 9 cut(s) 71, 215, 1263, 1321, 1545, 1962, 2240, 2381, 2649
Tsp45I GTSAC 2 cut(s) 2332, 2989
TspDTI ATGAA 6 cut(s) 288, 326, 1686, 1731, 2448, 3098
TspRI CASTG 8 cut(s) 271, 919, 1107, 1434, 2420, 2491, 2523, 3101
Van91I CCANNNNNTGG 2 cut(s) 2180, 2972
VpaK11BI GGWCC 4 cut(s) 435, 845, 989, 2183
XceI RCATGY 5 cut(s) 80, 727, 1666, 2692, 2879
XcmI CCANNNNNNNNNTGG 5 cut(s) 192, 861, 1821, 2231, 2412
XmaJI CCTAGG 2 cut(s) 1258, 1693
XmiI GTMKAC 1 cut(s) 2201
XmnI GAANNNNTTC 5 cut(s) 295, 882, 1052, 2425, 2439
ZrmI AGTACT 2 cut(s) 35, 1357
Zsp2I ATGCAT 1 cut(s) 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.